CN102988603A

CN102988603A - Wolfberry polysaccharide granules

Info

Publication number: CN102988603A
Application number: CN 201210412339
Authority: CN
Inventors: 罗瑞雪
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-10-25
Filing date: 2012-10-25
Publication date: 2013-03-27

Abstract

The invention provides wolfberry polysaccharide granules. The polysaccharide granules are characterized by being prepared from the following active pharmaceutical ingredients: 20-30g of wolfberry, 10-20g of mushroom and 5-10g of lucid ganoderma. A preparation method of the polysaccharide granules comprises the steps of weighing the ingredients according to the weights of the active pharmaceutical ingredients, grinding the ingredients into fine powder respectively, decocting wolfberry in a water bath at 90-95 DEG C, filtering the decoction with a 100-mesh sieve, concentrating the filtrate into extract, adding mushroom and lucid ganoderma fine powder, mixing the ingredients uniformly to prepare granules and drying the granules, thus obtaining the polysaccharide granules. The polysaccharide t granules has the advantages that the polysaccharide granules have the function of improving human immunity, are homology of medicine and food and do not have toxic or side effect.

Description

The lycium barbarum polysaccharide granule

Technical background:

Hepatic fibrosis (hepatic fibrosis) be in the various chronic hepatopathys progress since in the liver fiber generate with degraded unbalancely, cause too much collagen due to intrahepatic deposition, often with inflammation and can develop into hepatitis interstitialis chronica.The genesis of hepatic fibrosis is relevant with immune status.Research in recent years shows: immunoregulation plays an important role in hepatic fibrosis, this mainly is because the liver blood supply is abundant, it is the center that body carries out mass exchange and metabolism, easily contact with external foreign body antigen, thereby active cell immune system and immunoreation occurs, and the further release cells factor, activate fat-storing cell and cause collagenic supersession disorderly, cause the generation of hepatic fibrosis.The raising body immunity can stop the generation of hepatic fibrosis.

Lycium barbarum polysaccharide be from Fructus Lycii, extract and a kind of water soluble polysaccharide.Clearly this polysaccharide is proteoglycan, is become to be grouped into by arabinose, glucose, galactose, mannose, xylose, 6 kinds of monosaccharide of rhamnose.Show that after deliberation lycium barbarum polysaccharide is the main active that Fructus Lycii is regulated immunity, slow down aging, can improve the symptoms such as old people's fatiguability, inappetence and blurred vision, and have blood fat reducing, anti-fatty liver, the anti-ageing effect of waiting for a long time.Chinese patent (200310109427.7) discloses the application of a kind of lycium barbarum polysaccharide in preparation treatment Gastric Ulcer Treatment.This is invented described lycium barbarum polysaccharide and refer to separate LBP-2 and the LBP-4 that obtains from lycium barbarum polysaccharide.Zoopery shows, LBP-2 all has significant inhibitory action to the mouse gastric ulcer that the mouse gastric ulcer that caused by dehydrated alcohol and indometacin cause, the mouse gastric ulcer that LBP-4 causes dehydrated alcohol has significant inhibitory action, can be used as the medicine for the treatment of gastric ulcer.Other has patent (200810181505.7) to disclose the application of lycium barbarum polysaccharide in the prevention urinary stone.This invention shows that by experiment an amount of lycium barbarum polysaccharide can reduce drinks the kidney calcium oxalate stone formation that lures stone agent rat, and its mechanism of action may be by the antioxidation reaction, reduces the damage of radical pair nephridial tissue, thereby reduces the apoptosis of renal cells.Therefore the effect that has the prevention urinary stone, and it also has important application in medicine, food and field of health care products.Lycium barbarum polysaccharide has the characteristic of multiple obvious health care and integration of edible and medicinal herbs concurrently, and is with low cost, takes conveniently, and the patient compliance degree is high.Lycium barbarum polysaccharide belongs to pure natural substance simultaneously, and is without any side effects.

Summary of the invention:

The purpose of this invention is to provide a kind of lycium barbarum polysaccharide granule, have the effect that improves immunity.

The present invention adopts the crude drug such as Fructus Lycii 20-30g, Lentinus Edodes 10-20g, Ganoderma 5-10g to make, preparation method is, takes by weighing each component by the weight proportion of crude drug, respectively pulverization, Fructus Lycii is decocted 90-95 ℃ of water-bath, 100 orders filter, and filtrate concentrates the clear paste shape, adds Lentinus Edodes, Ganoderma fine powder, mixing, granulation, drying, and get final product.

Among the present invention lycium barbarum polysaccharide be from Fructus Lycii, extract and a kind of water soluble polysaccharide.Clearly this polysaccharide is proteoglycan, is become to be grouped into by arabinose, glucose, galactose, mannose, xylose, 6 kinds of monosaccharide of rhamnose.Show that after deliberation lycium barbarum polysaccharide is the main active that Fructus Lycii is regulated immunity, slow down aging, can improve the symptoms such as old people's fatiguability, inappetence and blurred vision, and have blood fat reducing, anti-fatty liver, the anti-ageing effect of waiting for a long time; Lentinus Edodes is second-biggest-in-the-world edible fungi, is rich in vitamin B group, ferrum, potassium, provitamin D (changing into vitamin D after Exposure to Sunlight), sweet in the mouth, and property is flat.Cure mainly loss of appetite, weak breath is weak; Ganoderma is Chinese traditional valuable ingredient of Chinese medicine, possesses very high medical value, and the modern pharmacology of process scientific research institution many decades studies confirm that, Ganoderma is regulated blood glucose for strengthening body immunity, the control blood pressure, auxiliary tumor chemoradiotherapy, hepatoprotective, the aspects such as hypnotic all have significant curative effect.

Advantage of the present invention is:

Have the effect that improves immunity, and integration of edible and medicinal herbs, have no side effect.

Specific embodiments:

Embodiment 1: take by weighing respectively the crude drug such as 20g, Lentinus Edodes 10g, Ganoderma 5g, with various crude drug difference pulverizations, Fructus Lycii is decocted 90-95 ℃ of water-bath, 100 orders filter, filtrate concentrates the clear paste shape, adds Lentinus Edodes, Ganoderma fine powder, mixing, granulation, drying, and get final product.

Embodiment 2: take by weighing respectively the crude drug such as 30g, Lentinus Edodes 20g, Ganoderma 10g, with various crude drug difference pulverizations, Fructus Lycii is decocted 90-95 ℃ of water-bath, 100 orders filter, filtrate concentrates the clear paste shape, adds Lentinus Edodes, Ganoderma fine powder, mixing, granulation, drying, and get final product.

Embodiment 3: take by weighing respectively the crude drug such as 25g, Lentinus Edodes 15g, Ganoderma 8g, with various crude drug difference pulverizations, Fructus Lycii is decocted 90-95 ℃ of water-bath, 100 orders filter, filtrate concentrates the clear paste shape, adds Lentinus Edodes, Ganoderma fine powder, mixing, granulation, drying, and get final product.

Embodiment 4: adopt mice reticuloendothelial system phagocytic functional experiment, research lycium barbarum polysaccharide granule strengthens the effect of cellular immune function.

Adopt mice as animal subject, get 50 mices, male and female half and half, body weight are 18-22 g, are divided at random 5 groups: high dose group, middle dosage group, low dose group, and positive controls, model group, every group of l0 is only.Model group is according to the dosage gavage normal saline of 0.2ml/10g; Positive controls gavage 2.0g/kg lycium barbarum polysaccharide oral liquid; High, normal, basic dosage group gives respectively lycium barbarum polysaccharide granule 2.0g/kg, 1.0 g/kg, 0.5g/kg gavage, successive administration 20 days.30min gets and respectively organizes mice after the last administration, 25% india ink of tail vein injection 1% gelatin solution dilution, administration volume 0. 1ml/10g.30s and 6min mice vena orbitalis posterior clump are got blood 0. 025ml behind the injection prepared Chinese ink, be blown at once among the 0. 1%Na2CO3 liquid 2ml, suction pipe sucks in this liquid, blows out for several times, the blood that adheres to fully to wash out the suction pipe wall, it is complete to get blood, be dissolved in 2ml0. 1%Na2CO3 liquid school zero, 675nm place colorimetric on spectrophotometer with 0.025ml normal mouse blood.And after for the second time blood sampling, with taking off the cervical vertebra method mice is put to death, weigh, get liver, spleen is weighed, be calculated as follows phagocytic index k and engulf coefficient (correction phagocytic index) A:K=(lgOD1-lgOD2)/(T2-T1), A=W (K) ^1/3OD1, OD2 are 30s and 6min optical density in/the WLS formula, and T is the time, and W is body weight, and WLS is that the liver spleen closes heavily.

The result: such as table 1, the basic, normal, high dosage of lycium barbarum polysaccharide granule all can strengthen the mice reticuloendothelial system to the ability of cleaning up of engulfing of inertia carbon granule in the blood flow, and K value and A value are all seen obviously and increased (P＜0.05).

The impact of table 1 lycium barbarum polysaccharide Granules on Mouse reticuloendothelial system phagocytic function (means ± s)

?	Medicine	Dosage	The animal number of elements	Phagocytic index k	Proofread and correct phagocytic index A
						Model group	Normal saline	0.2ml/10g	10	0. 0102±0.0011	3. 42 ± 0. 62
Positive group	The lycium barbarum polysaccharide oral liquid	2.0g/kg	10	0. 0311±0.0082*	6. 02 ± 1.23*
						High dose group	The lycium barbarum polysaccharide granule	2.0g/kg	10	0. 0325±0.0074*	6. 24 ± 1.52*
Middle dosage group	The lycium barbarum polysaccharide granule	1.0g/kg	10	0. 0295±0.0083*	5. 86 ± 0. 74*
						Low dose group	The lycium barbarum polysaccharide granule	0.5g/kg	10	0. 0242±0.0086*	4.91 ± 0. 80*

* p＜0.05, * * p＜0.01 is compared with model group

Conclusion: the lycium barbarum polysaccharide granule can strengthen mice reticuloendothelial system phagocytic function, promotes the effect of cellular immune function.

Embodiment 5: adopt the determination experiment of half hemolysis value (HC50), research lycium barbarum polysaccharide granule strengthens the effect of mouse humoral immune function.

Adopt mice as animal subject, get 50 mices, male and female half and half, body weight are 18-22 g, are divided at random 5 groups: high dose group, middle dosage group, low dose group, and positive controls, model group, every group of l0 is only.Model group is according to the dosage gavage normal saline of 0.2ml/10g; Positive controls gavage 2.0g/kg lycium barbarum polysaccharide oral liquid; High, normal, basic dosage group gives respectively lycium barbarum polysaccharide granule 2.0g/kg, 1.0 g/kg, 0.5g/kg gavage, successive administration 20 days.The 8th day initial immunity of administration, sheep red blood cell (SRBC) (SRBC) 0.2mL of 5 groups of equal lumbar injections 5% of mice, administration again immunity in the 15th day, dosage is the same.The 20th day, after the last administration behind the 30min, mice vena orbitalis posterior clump is got blood 1ml, centrifugal, get serum, with the SA buffer serum is diluted 200 times, getting 1mL puts in vitro, add successively 10% (v/v is with the preparation of SA buffer) hematocrit SRBC 0.5 mL, complement 1mL (pressing the 1B10 dilution with the SA buffer).Other establishes the not control tube of increase serum (replacing with the SA buffer).After putting in 37 ℃ of waters bath with thermostatic control insulation 30min, the ice bath cessation reaction.The centrifugal 10min of 2000 rpm gets supernatant 1 mL, adds Dou Shi reagent 3 mL.(v/ v is with the preparation of SA buffer) hematocrit SRBC 0.25 mL that gets simultaneously 10%, add Dou Shi reagent to 4mL in another test tube, abundant mixing, place 10 min after, sentence control tube in 540nm and do blank, measure respectively and respectively manage light absorption value, calculate half hemolysis value HC50.The amount of hemolysin is calculated as follows with half hemolysis value (HC50) expression: the light absorption value * extension rate during half hemolysis value (HC50)=sample light absorption value/SRBC HD50.

Result: such as table 2.Compare with the model group mice, the basic, normal, high dosage of lycium barbarum polysaccharide granule makes antibody forming cell OD value increase (P＜0.05), serum hemolysin HC50 raise (P＜0.05).

Table 2 lycium barbarum polysaccharide granule is on the impact of HC50 (means ± s)

?	Medicine	Dosage	The animal number of elements	OD	HC50（g/Kg）
						Model group	Normal saline	0.2ml/10g	10	0. 425± 0. 032	109.52± 22.26
Positive group	The lycium barbarum polysaccharide oral liquid	2.0g/kg	10	0.570± 0. 121*	148.50± 35.01*
						High dose group	The lycium barbarum polysaccharide granule	2.0g/kg	10	0. 624± 0. 132*	154.14± 41.13*
Middle dosage group	The lycium barbarum polysaccharide granule	1.0g/kg	10	0. 583± 0. 119*	142.23± 40.23*
						Low dose group	The lycium barbarum polysaccharide granule	0.5g/kg	10	0. 544± 0. 094*	134.35± 31.42*

* p＜0.05, * * p＜0.01 is compared with model group

Conclusion: lycium barbarum polysaccharide granule energy enhancing antibody forms cell OD value and serum hemolysin HC50 increases, and strengthens the effect of humoral immune function.

Claims

1. a lycium barbarum polysaccharide granule is characterized in that: be comprised of following crude drug: Fructus Lycii 20-30g, Lentinus Edodes 10-20g, Ganoderma 5-10g.

2. according to claims 1 described lycium barbarum polysaccharide preparation method of granules, it is characterized in that: the weight proportion by crude drug takes by weighing each component, pulverization decocts Fructus Lycii 90-95 ℃ of water-bath respectively, and 100 orders filter, filtrate concentrates the clear paste shape, add Lentinus Edodes, Ganoderma fine powder, mixing, granulation, drying, and get final product.