CN102406172B - Health-care food for enhancing immunity and preparation method thereof - Google Patents
Health-care food for enhancing immunity and preparation method thereof Download PDFInfo
- Publication number
- CN102406172B CN102406172B CN2011103848305A CN201110384830A CN102406172B CN 102406172 B CN102406172 B CN 102406172B CN 2011103848305 A CN2011103848305 A CN 2011103848305A CN 201110384830 A CN201110384830 A CN 201110384830A CN 102406172 B CN102406172 B CN 102406172B
- Authority
- CN
- China
- Prior art keywords
- hawthorn
- date
- ant
- health
- mouse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000036039 immunity Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 230000002708 enhancing effect Effects 0.000 title abstract description 6
- 235000013305 food Nutrition 0.000 title abstract description 6
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims abstract description 23
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims abstract description 23
- 235000009685 Crataegus X maligna Nutrition 0.000 claims abstract description 23
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims abstract description 23
- 235000009486 Crataegus bullatus Nutrition 0.000 claims abstract description 23
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims abstract description 23
- 235000009682 Crataegus limnophila Nutrition 0.000 claims abstract description 23
- 235000004423 Crataegus monogyna Nutrition 0.000 claims abstract description 23
- 235000002313 Crataegus paludosa Nutrition 0.000 claims abstract description 23
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 235000006545 Ziziphus mauritiana Nutrition 0.000 claims abstract description 8
- 235000008529 Ziziphus vulgaris Nutrition 0.000 claims abstract description 8
- 241001092040 Crataegus Species 0.000 claims description 29
- 239000009636 Huang Qi Substances 0.000 claims description 28
- 235000013402 health food Nutrition 0.000 claims description 25
- 244000241872 Lycium chinense Species 0.000 claims description 21
- 235000015468 Lycium chinense Nutrition 0.000 claims description 21
- 235000013399 edible fruits Nutrition 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 19
- 239000000284 extract Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 9
- 239000008187 granular material Substances 0.000 claims description 8
- 235000008440 Crataegus cuneata Nutrition 0.000 claims description 7
- 235000014283 Crataegus pinnatifida var major Nutrition 0.000 claims description 7
- 240000000038 Ziziphus mauritiana Species 0.000 claims description 7
- 239000002775 capsule Substances 0.000 claims description 7
- 239000003826 tablet Substances 0.000 claims description 4
- 239000002552 dosage form Substances 0.000 claims description 2
- 241000545442 Radix Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 16
- 210000000952 spleen Anatomy 0.000 abstract description 13
- 238000002474 experimental method Methods 0.000 abstract description 11
- 238000005728 strengthening Methods 0.000 abstract description 10
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 206010020751 Hypersensitivity Diseases 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 230000001717 pathogenic effect Effects 0.000 abstract description 4
- 210000003734 kidney Anatomy 0.000 abstract description 3
- 206010028980 Neoplasm Diseases 0.000 abstract description 2
- 240000000171 Crataegus monogyna Species 0.000 abstract 1
- 235000017784 Mespilus germanica Nutrition 0.000 abstract 1
- 244000182216 Mimusops elengi Species 0.000 abstract 1
- 235000000560 Mimusops elengi Nutrition 0.000 abstract 1
- 235000007837 Vangueria infausta Nutrition 0.000 abstract 1
- 244000126002 Ziziphus vulgaris Species 0.000 abstract 1
- 208000030961 allergic reaction Diseases 0.000 abstract 1
- 229940107666 astragalus root Drugs 0.000 abstract 1
- 239000002158 endotoxin Substances 0.000 abstract 1
- 230000007774 longterm Effects 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 42
- 210000004027 cell Anatomy 0.000 description 23
- 239000013642 negative control Substances 0.000 description 15
- 239000000706 filtrate Substances 0.000 description 13
- 239000000243 solution Substances 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 210000003743 erythrocyte Anatomy 0.000 description 9
- 239000002504 physiological saline solution Substances 0.000 description 9
- 241000287828 Gallus gallus Species 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 230000000242 pagocytic effect Effects 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 210000002784 stomach Anatomy 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 239000012980 RPMI-1640 medium Substances 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 5
- 239000000976 ink Substances 0.000 description 5
- 210000002540 macrophage Anatomy 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 108010006464 Hemolysin Proteins Proteins 0.000 description 4
- 208000026935 allergic disease Diseases 0.000 description 4
- 230000007815 allergy Effects 0.000 description 4
- 230000003111 delayed effect Effects 0.000 description 4
- 239000003228 hemolysin Substances 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 4
- 210000003024 peritoneal macrophage Anatomy 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- LOTKRQAVGJMPNV-UHFFFAOYSA-N 1-fluoro-2,4-dinitrobenzene Chemical compound [O-][N+](=O)C1=CC=C(F)C([N+]([O-])=O)=C1 LOTKRQAVGJMPNV-UHFFFAOYSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000005534 hematocrit Methods 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000004988 splenocyte Anatomy 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 206010013183 Dislocation of vertebra Diseases 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 241001620634 Roger Species 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003266 anti-allergic effect Effects 0.000 description 2
- 230000001986 anti-endotoxic effect Effects 0.000 description 2
- 230000002180 anti-stress Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 210000001508 eye Anatomy 0.000 description 2
- 206010016256 fatigue Diseases 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000006025 fining agent Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 235000012907 honey Nutrition 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 108010011485 Aspartame Proteins 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000026010 Dendrobium candidum Species 0.000 description 1
- 206010014025 Ear swelling Diseases 0.000 description 1
- 241001420794 Formica rufa Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 241000051764 Lasius umbratus Species 0.000 description 1
- 101000981253 Mus musculus GPI-linked NAD(P)(+)-arginine ADP-ribosyltransferase 1 Proteins 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 244000197580 Poria cocos Species 0.000 description 1
- 235000008599 Poria cocos Nutrition 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 1
- 229960003438 aspartame Drugs 0.000 description 1
- 235000010357 aspartame Nutrition 0.000 description 1
- 239000000605 aspartame Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- CJDPJFRMHVXWPT-UHFFFAOYSA-N barium sulfide Chemical compound [S-2].[Ba+2] CJDPJFRMHVXWPT-UHFFFAOYSA-N 0.000 description 1
- -1 be 25ml) Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 210000003298 dental enamel Anatomy 0.000 description 1
- 230000035617 depilation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000007160 gastrointestinal dysfunction Effects 0.000 description 1
- 235000020710 ginseng extract Nutrition 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000036299 sexual function Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000004206 stomach function Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000009495 sugar coating Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to health-care food for enhancing immunity and a preparation method thereof. The food contains the following raw materials in part by weight: 10 to 30 parts of astragalus root, 30 to 40 parts of Chinese date, 3 to 40 parts of hawthorn, 0.4 to 0.6 part of medlar, and 0.4 to 0.6 part of ant. The health-care food can be in various conventional oral preparation forms containing or not containing medicinal auxiliary materials. The health-care food is obtained according to the principles of strengthening body resistance and eliminating pathogenic factors in the Chinese medicine, animal experiments and multiple formula screening and has the effects of strengthening spleen and kidneys, strengthening body resistance and consolidating the constitution, resisting oxidation and stress damage, resisting endotoxin reaction and allergic reaction and resisting mutation and cancers, and can play roles in enhancing the immunity of the body and delaying senility after long-term administration.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof, particularly relate to a kind of for health food that strengthens immunity and preparation method thereof.
Background technology
Immune response a kind of defensive reaction that to be body take place the foreign matter material is the distinctive a kind of identification of body and the process of eliminating allosome, to keep the stable state of environment in the health.These foreign matters comprise: the cell of the aging death that the mutant cell that various pathogenic microorganisms, environmental factor are brought out and body metabolism produce.Social now, the sharp increase of work and life stress is accompanied by the pollution of environment and diet, make immunity of organisms sharply descend and be in inferior clinical symptom, as failure of memory, lassitude, fatigue, easy catching a cold, gastrointestinal dysfunction even the generation of disease of cardiovascular system, diabetes and tumour occurs.Developmental research can strengthen the health food of body's immunity to prophylactic generation, delays the life-span, and the life quality that improves population of China has great social significance.
Circulate on the domestic market at present through state food drug surveilance office (SFDA) approval be that the health food of major function is many to strengthen immunity, for example: the strong board dendrobium candidum tea of boat, the sun are given birth to board genseng health unit of section powder, day source hall board bee glue capsule etc.Although these health foods, are safeguarded healthy aspect at the enhancing body immunity and have been brought into play positive effect, mostly with single raw material components, the compatibility principle that does not possess the traditional Chinese medical science, can't reach the effect that strengthens immunity from the theory of traditional Chinese medical science aspect, and price is higher, can not meet the need of market.
Number of patent application is 200910091300.4 Chinese patent application, open day is 2010.2.3 day, disclose a kind of for the health food that strengthens immunity, it contains ginseng extract, Fructus lycii P.E, Radix Polygoni Multiflori Preparata extract, black-bone chicken extract, have that raw material is natural, the characteristics of edible safety, comprehensive nutrition, but cost is higher, and price is more expensive.
Number of patent application is 201110104696.9 Chinese patent application, open day is 2011.9.14 day, a kind of health food that has the strengthening the spleen and stomach function and strengthen immunity is disclosed, it contains Poria cocos, hawthorn, Asparagus, date, white fungus, mushroom, has the effect that replenishes qi to invigorate the spleen with stomach, gentle coordinating intestines and stomach, be particularly suitable for children taking, but its effect is gentle, the effect that strengthens immunity is still waiting to improve.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of the have body's immunity of enhancing and anti-aging health food and preparation method thereof, and this health food cost is lower and preparation technology is simple.
For reaching above-mentioned purpose, a kind of health food that strengthens immunity of the present invention, it contains the raw material components of following weight portion: Radix Astragali 10-30 part, date 30-40 part, hawthorn 3-40 part, fruit of Chinese wolfberry 0.4-0.6 part, ant 0.4-0.6 part; Preferred Radix Astragali 15-25 part, date 35-40 part, hawthorn 20-30 part, fruit of Chinese wolfberry 0.45-0.55 part, ant 0.45-0.55 part; More preferably the Radix Astragali is 20 parts, 35 parts in date, 30 parts of hawthorn, 0.5 part of the fruit of Chinese wolfberry, 0.5 part of ant.
The used bulk drug of the present invention all can be bought from common Chinese material shop and obtain, and its specification meets national Chinese medicine standard and gets final product.
A kind of health food that strengthens immunity of the present invention, it can be the various conventional oral formulations form that contains or do not contain medicinal auxiliary material, wherein preferred described preparation is tablet, granule, capsule or oral liquid, wherein used pharmaceutic adjuvant is the various pharmaceutic adjuvants that usefulness is produced in this area, as flavouring, stabilizing agent, thickener etc., it meets, and " standard of Chinese pharmacopoeia version pharmaceutic adjuvant in 2010 gets final product.
A kind of method for preparing above-mentioned health food, may further comprise the steps: the Radix Astragali is cleaned, and date, hawthorn are cleaned stoning, and the Radix Astragali decocts 2-5 time, add the water that 10-30 doubly measures at every turn, decocted 1-2 hour, and merged extract, in extract, add the fruit of Chinese wolfberry, date, hawthorn and ant, decocted 30-120 minute, filter, add pharmaceutic adjuvant, make following different dosage form respectively: tablet, granule, capsule or oral liquid.Health food of the present invention is prepared into various conventional formulation forms, is this area conventional method.
The way to keep in good health of motherland's medical science is paid attention to internal cause, has both relied on the outer evil intrusion of immunocompetence antagonism that improves body self, and namely what is called is set upright and got rid of evils.What is called is set upright, and refers to that benefit is defended gas, tonifying primordial Qi, the gas that nourishes blood, and namely meaning is transferred the resistance against diseases of body, improves body's immunological function.What is called is got rid of evils, refer to disperse ailment said due to cold or exposure, clearing heat and detoxicating, activate blood circulation and disperse blood clots, resist the principles of reatment such as invasion and attack of external adverse factor, by suppressing immune response and regulating the resistance against diseases that immunologic balance improves body.Be the square medicine that the diagnosis and treatment based on an overall analysis of the illness and the patient's condition rule of principle is carried out compatibility with the strengthening vital QI to eliminate pathogenic factors, can act on the immune decorum, performance is to the preventive and therapeutic effect of immunity disease.
The present invention starts with from the reason that causes body's immunity to descend, and adopts the Radix Astragali, hawthorn, the fruit of Chinese wolfberry and ant to strengthen the immunocompetence of body; The effect of the anti-oxidant and anti-stress damage of the Radix Astragali, date, hawthorn and the fruit of Chinese wolfberry; Hawthorn and the fruit of Chinese wolfberry reduce blood fat; The Radix Astragali and the reaction of date antiendotoxin and antiallergic action; The anti-sudden change of date and antitumaous effect; The Radix Astragali and ant strengthening spleen, tonifying kidney, clearing and activating the channels and collaterals, improve endurance antifatigue enhancing sexual function and delay senility.The present invention is that the diagnosis and treatment based on an overall analysis of the illness and the patient's condition rule of principle is carried out compatibility with the strengthening vital QI to eliminate pathogenic factors, obtains health food of the present invention, and it has important health care's effect to strengthening immunity of organisms and delaying senility, and has no side effect, and cost is lower, and the preparation method is simple.
The specific embodiment
Below in conjunction with embodiment and test data, be described in more detail with other technical characterictic and advantage the present invention is above-mentioned.
Embodiment 1
The accurate following raw material of weighing:
Radix Astragali 100g, date 300g, hawthorn 30g, fruit of Chinese wolfberry 4g, ant 4g (Polyhachis vicina Roger is available from pharmacy of Tongrentang).
The Radix Astragali is cleaned, and date, hawthorn are cleaned stoning, and the Radix Astragali decocts 2 times, adds the water of 1000g at every turn, decocts 1 hour, merges extract, adds the fruit of Chinese wolfberry, date, hawthorn and ant in extract, decocts 30 minutes, filters, and obtains filtrate.In filtrate, add excipient 30g (wherein containing starch 15g, talcum powder 13g, 95% ethanol 2g), granulate with comminutor, the tablet press machine compressing tablet, sugarcoating machine wrap film clothing namely gets tablet.
Embodiment 2
The accurate following raw material of weighing:
Radix Astragali 300g, date 400g, hawthorn 400g, fruit of Chinese wolfberry 6g, ant (ant is deceived on the Changbai Mountain, available from pharmacy of Tongrentang) 6g.
The Radix Astragali is cleaned, and date, hawthorn are cleaned stoning, and the Radix Astragali decocts 3 times, adds the water of 4500g at every turn, decocts 1.5 hours, merges extract, adds the fruit of Chinese wolfberry, date, hawthorn and ant in extract, decocts 60 minutes, filters, and obtains filtrate.Add excipient 105g (wherein containing 50g starch, 50g talcum powder, 95% ethanol 5g) in filtrate, lactose or Aspartame 50g stir, granulate with comminutor, and drying, whole grain, pack namely gets granule.
Embodiment 3
The accurate following raw material of weighing:
Radix Astragali 150g, date 350g, hawthorn 200g, fruit of Chinese wolfberry 4.5g, ant (red ant is available from pharmacy of Tongrentang) 4.5g.
The Radix Astragali is cleaned, and date, hawthorn are cleaned stoning, and the Radix Astragali decocts 4 times, adds the water of 3000g at every turn, decocts 1.5 hours, merges extract, adds the fruit of Chinese wolfberry, date, hawthorn and ant in extract, decocts 80 minutes, filters, and obtains filtrate.In filtrate, add excipient 30g (wherein containing starch 15g, talcum powder 13g, 95% ethanol 2g), stir, granulate with comminutor, make capsule with the capsule filler.
Embodiment 4
The accurate following raw material of weighing:
Radix Astragali 250g, date 400g, hawthorn 300g, fruit of Chinese wolfberry 5.5g, ant (yellow ant is available from pharmacy of Tongrentang) 5.5g.
The Radix Astragali is cleaned, and date, hawthorn are cleaned stoning, and the Radix Astragali decocts 5 times, adds the water of 7500g at every turn, decocts 2 hours, merges extract, adds the fruit of Chinese wolfberry, date, hawthorn and ant in extract, decocts 120 minutes, filters, and obtains filtrate.Filtrate is concentrated into 2500mL with Rotary Evaporators, add fining agent (shitosan or ethanol, the volume that adds is 1% of the volume that concentrates rear filtrate, be 25ml), lactose or refined honey 150g, citric acid (add the volume number for concentrating 0.3% of rear filtrate weight, be 0.45ml) after, be filled into 5mL, 10mL, the brown oral liquid bottle of 50mL, namely get oral liquid.
Embodiment 5
The accurate following raw material of weighing:
Radix Astragali 200g, date 350g, hawthorn 300g, fruit of Chinese wolfberry 5g, ant (Polyhachis vicina Roger is available from pharmacy of Tongrentang) 5g.
The Radix Astragali is cleaned, and date, hawthorn are cleaned stoning, and the Radix Astragali decocts 3 times, adds the water of 4000g at every turn, decocts 1.5 hours, merges extract, adds the fruit of Chinese wolfberry, date, hawthorn and ant in extract, decocts 80 minutes, filters, and obtains filtrate.Filtrate is concentrated into 2500mL with Rotary Evaporators, behind adding fining agent (shitosan or ethanol 25ml), lactose or refined honey 150g, the citric acid 0.45ml, is filled into 5mL, 10mL, the brown oral liquid bottle of 50mL, namely get oral liquid.
Health food of the present invention calculates with adult 60kg, and dosage is every day: 2.4g/60kg, amounts to 40mg/kg.
The test example
Test method:
1. animal used as test and grouping: healthy 200 of the Kunming mouses (male and female dual-purpose) of SPF level, body weight is 18～22 grams, is provided by Harbin Medical University's Experimental Animal Center.Per 40 mouse are 1 group, totally 5 groups.The I group is carried out mouse lymphocyte transformation experiment, NK cytoactive mensuration that ConA induces; The II group is carried out the delayed allergy experiment; The III group is carried out serum hemolysin mensuration and antibody-producting cell number and is measured; The IV group is carried out carbon and is cleaned up experiment; The V group is carried out Turnover of Mouse Peritoneal Macrophages and is engulfed the chicken red blood cell experiment.
2. experimental situation condition: temperature: 22～25 ℃, relative humidity: 55～70%.
3. dosage is selected and is tried thing to give mode: recommend consumption according to human oral, if the basic, normal, high dosage group of sample is respectively 200,400,800mg/kg BW (be equivalent to human body respectively and recommend 5,10,20 times of consumption), negative control group, every group of 10 animals.Take by weighing the filtrate that obtains in above-described embodiment respectively, be made into 10,20,40mg/mL concentration solution, give the corresponding dosage treated animal respectively and irritate stomach, irritate the long-pending 0.2mL/10g BW of being of body of stomach, negative control group gives isopyknic pure water, irritates stomach every day once, continuous irrigation stomach 30 days.
Experimental example 1: health food of the present invention is to the influence of the cellular immunity of mouse
1.ConA the mouse spleen lymphocyte transformation experiment (mtt assay) of inducing
The aseptic spleen of getting places the plate that fills an amount of aseptic Hank ' s liquid, makes cell suspension, filters through 200 eye mesh screens.Use Hank ' s liquid to wash 2 times, each centrifugal 10min (1000r/min).Then cell is suspended in the 1mL complete culture solution, the living cell counting number, adjusting cell concentration with the RPMI1640 nutrient solution is 3 * 10
6Individual/mL.Again cell suspension is divided two holes to add in 24 well culture plates, every hole 1mL, a hole adds 75 μ LConA liquid (being equivalent to 7.5 μ g/mL) therein, and 5%CO is put in contrast in another hole
2, cultivate 72h in 37 ℃ of carbon dioxide incubators.Cultivate and finish preceding 4h, supernatant 0.7mL is inhaled in every hole gently, adds the RPMI1640 nutrient solution that 0.7mL does not contain calf serum, adds MTT (5mg/mL) 50 μ L/ holes simultaneously, continues to cultivate 4h.After cultivating end, every hole adds 1mL acid isopropyl alcohol, and the piping and druming mixing dissolves purple crystal fully.Divide then to install in 96 well culture plates, 3 parallel holes are made in each hole, use ELIASA, measure OD value with the 570nm wavelength.Lymphocytic competence for added value deducts the OD value that does not add the ConA hole with the OD value that adds the ConA hole and represents.
The results are shown in Table 1: the lymphocyte transformation ability of each dosage group mouse of sample all is higher than negative control group, and the difference of each dosage group and negative control group all has highly significant (P＜0.01), shows that this sample has the lymphopoiesis that promotes mouse, the effect of conversion capability.
The influence of the mouse spleen lymphocyte conversion capability that table 1 health food of the present invention is induced ConA
2. dinitrofluorobenzene mouse delayed allergy (DTH) experiment (ear swelling method) of inducing
Experiment finish (whole experiment needs 7-8 days) preceding 5 days with barium sulphide with mouse part skin depilation about 3cm * 3cm scope, evenly smear sensitization with 50 μ L dinitrofluorobenzene (DNFB) solution, after 5 days 10 μ LDNFB are evenly smeared in the mouse right ear two sides and attack, mouse is put to death in the cervical vertebra dislocation after 24 hours, cut left and right sides auricular concha, with card punch take off the 8mm diameter auricle, weigh, represent the degree of DTH with the difference of left and right sides ear weight.
The results are shown in Table 2, the left and right sides auricle weight difference of sample height, middle dosage group mouse is higher than negative control group, high dose group wherein and the difference of negative control group have highly significant (P＜0.01), show that this sample has the effect of the delayed allergy that promotes mouse.
Mouse delayed allergy (DTH) experimental result of table 2 the present invention prescription
Experimental example 2: health food of the present invention is to the influence of the humoral immunity of mouse
1. antibody-producting cell detects (Jerne improves slide method)
Get the sheep blood of defiber, with physiological saline washing 3 times, each centrifugal 10min (2000r/min) is made into the cell suspension of 2% (v/v), every mouse lumbar injection 0.2mL with physiological saline with hematocrit SRBC.The mouse that immunity is back 5 days is put to death, and gets spleen and puts in the plate that fills Hank ' s liquid, grinds spleen gently, make cell suspension, filter centrifugal 10min (2000r/min) through 200 eye mesh screens, use Hank ' s liquid washing 2 times, at last cell is suspended in 8mL Hank ' the s liquid.After top layer culture medium (the 1g agarose adds distilled water to 100mL) heating for dissolving, put 45～50 ℃ of water bath heat preservations, mix with Hank ' the s liquid of equivalent pH7.2～7.42 times concentration, the packing small test tube, every pipe 0.5mL, in pipe, add 50 μ L 10%SRBC (v/v again, with the preparation of SA buffer solution), 25 μ L splenocyte suspensions are poured on the slide of brushing the agarose thin layer behind the mixing rapidly, do parallel plate, after treating that agar solidifies, the flat button of slide is placed on the slide frame, puts into CO2gas incubator and hatch 1.5h, in complement (1: 8) the adding slide frame groove with the dilution of SA buffer solution, continue to hatch 1.5h, counting hemolysis plaque number.Represent the antibody-producting cell number with plaque number/full splenocyte.
The results are shown in Table 3, the antibody-producting cell number average of each dosage group mouse of sample is higher than negative control group, the difference of height wherein, middle dosage group and negative control group has highly significant (P＜0.01), shows that this sample has the effect of the antibody-producting cell propagation that promotes mouse.
The antibody-producting cell test experience result of table 3 the present invention prescription
2. the mensuration of serum hemolysin (blood clotting method)
Get the sheep blood of defiber, with physiological saline washing 3 times, each centrifugal 10min (2000r/min) is made into the cell suspension of 2% (v/v), every mouse lumbar injection 0.2mL with physiological saline with hematocrit SRBC.After the immunity 5 days, extract the eyeball of mouse, get blood in centrifuge tube, place about 1h, the centrifugal 10min of 2000r/min separates, collects serum.With the serum doubling dilution, the dilution serum of difference is placed in the Microhemagglutination plate every hole 100 μ L respectively with physiological saline, the SRBC suspension that adds 100 μ L 0.5% (v/v) again, mixing is added a cover in the moistening square position of packing into, in 37 ℃ of incubation 3h, observe the hemagglutination degree.Be calculated as follows the antibody product:
The antibody product=(S1+2S2+3S3......nSn)
In the formula 1,2,3......n is two-fold dilution's index, S is the rank of aggegation degree.
The results are shown in Table 4, the antibody product of each dosage mouse of sample all is higher than negative control group, and the difference of height wherein, middle dosage group and negative control group has highly significant (P＜0.01), shows that this sample has the effect of the serum hemolysin level that improves mouse.
The mouse hemolysin measurement result of table 4 the present invention prescription
3. the influence that the monokaryon of mouse-macrophage carbon is cleaned up (mouse carbon is cleaned up experiment)
Through the tail vein to the india ink of injected in mice with 4 times of physiological saline dilutions, every 10g body weight injection 0.1mL, timing immediately after prepared Chinese ink injects after injecting prepared Chinese ink the 2nd, 10min, is got blood 20 μ L from the angular vein clump respectively, joins 2mL0.1%Na
2CO
3In the solution, shake up.Na with 0.1%
2CO
3Solution is made blank, uses ultraviolet-uisible spectrophotometer with 600nm wavelength photometry density value (OD).Mouse is put to death, get liver, spleen, weigh.Be calculated as follows phagocytic index a.
A=K
1/3* body weight/(liver weight+spleen is heavy)
Clean up index K=(lgOD in the formula
1-lgOD
2)/(t
2-t
1)
OD
1Be t
1The time absorbance, OD
2Be t
2The time absorbance; t
1For giving the China ink time that blood is got for the first time in the back, t
2For giving the China ink time that blood is got for the second time in the back.
The results are shown in Table 5, the phagocytic index of each dosage mouse of sample and negative control group relatively, there are no significant for difference (P＞0.05), show this sample to mouse monokaryon-macrophage carbon is cleaned up function does not have obvious facilitation.
The carbon of the mouse of table 5 health food of the present invention is cleaned up experimental result
4. Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method)
Hematocrit chicken erythrocyte suspension to mouse peritoneal injection 20% (v/v: prepare with physiological saline), every mouse 1mL, interval 30min, mouse is put to death in the cervical vertebra dislocation, and abdominal skin is cut off in the center, the abdominal cavity injects 2mL physiological saline, rotate mouse plate 1min, sucking-off abdominal cavity washing lotion 1mL, mean droplet is on 2 slides, put into the enamel box that is lined with wet gauze, put 37 ℃ of incubator incubation 30min.Incubate completely, take out slide and dry after the rinsing in physiological saline, use methyl alcohol: acetone (1: 1) solution is fixed, and 4% (v/v) Giemsa-phosphate buffer dyes, again with the pure water rinsing, dry.100 macrophages of every counting are calculated as follows phagocytic rate and phagocytic index under the oil mirror.
Phagocytic rate (%)=engulf macrophage * 100% of the macrophage number/counting of chicken red blood cell
The macrophage number of chicken red blood cell sum/counting that phagocytic index=quilt is engulfed
The results are shown in Table 6, the peritoneal macrophage of each dosage group mouse of sample all is higher than negative control group to phagocytic rate and the phagocytic index of chicken red blood cell, wherein high, middle dosage group and negative control group difference have highly significant (P＜0.01), show that this sample has the effect of the phagocytosis of macrophages that promotes mouse.
The Turnover of Mouse Peritoneal Macrophages of table 6 the present invention prescription is engulfed the chicken red blood cell experimental result
5. sample is to the influence (LDH determination method) of the NK cytoactive of mouse
The dislocation of mouse cervical vertebra is put to death, and the aseptic spleen of getting is made splenocyte suspension, uses Hank ' s liquid to wash 2 times, each centrifugal 10min (1000r/min).Abandon supernatant, cytoplasm is upspring, added the 0.5mL aqua sterilisa 20 seconds, add 0.5mL 2 times of Hank ' s liquid and 8mL Hank ' s liquid after the splitting erythrocyte again, the centrifugal 10min of 1000r/min, the RPMI1640 complete culture solution that contains 10% calf serum with 1mL is resuspended, with 1% glacial acetic acid dilution back counting (red blood cell number should more than 95%), with the blue dyeing counting viable count of platform phenol, adjusting cell concentration with the RPMI1640 complete culture solution at last is 2 * 10
7Individual/mL, this is the effector cell.Get the well-grown YAC-1 cell of back 24h that goes down to posterity, adjusting cell concentration with the RPMI1640 complete culture solution is 4 * 10
5Individual/mL, this is target cell.Get each 100 μ L of target cell and effector cell (imitating target than 50: 1), add in U-shaped 96 well culture plates; Target cell nature release aperture adds target cell and nutrient solution 100 a μ L, and the maximum release aperture of target cell adds target cell and each 100 μ L of 1%NP40.Above-mentioned every 3 parallel holes of respectively establishing are put 5%CO
2, cultivate 4h in 37 ℃ of carbon dioxide incubators, then with 96 well culture plates with the centrifugal 5min of 1500r/min, draw at the bottom of the supernatant 100 μ L horizontalizations in 96 well culture plates in every hole, add LDH matrix liquid 100 μ L simultaneously, reacted 8 minutes, every hole adds the HCL30 μ L of 1mol/L, measures optical density (OD) value at ELIASA 490nm place.Be calculated as follows the NK cytoactive.
NK cytoactive (%)=(reacting hole OD-nature release aperture OD)/(maximum release aperture OD-nature release aperture OD) * 100%.
The results are shown in Table 7 as seen, the NK cytoactive of each dosage group mouse of sample all is higher than negative control group, but the difference of each dosage group and negative control group there are no significant (P＞0.05), show that this sample does not have obvious influence to the NK cytoactive of mouse.
The NK cells in mice determination of activity result of table 7 the present invention prescription
Health food of the present invention is to obtain according to the principle of traditional Chinese medical science strengthening vital QI to eliminate pathogenic factors and animal experiment and the examination of repeatedly writing out a prescription, have strengthening spleen, tonifying kidney, strengthen the body resistance to consolidate the constitution, the effect of anti-oxidant and anti-stress damage, antiendotoxin reaction and antiallergic action and anti-sudden change and antitumaous effect, can play and strengthen immunity of organisms and function in delaying senility.
Above-described embodiment is described preferred embodiment of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (3)
1. a health food that strengthens immunity is characterized in that, in weight portion, its raw material consists of: Radix Astragali 10-30 part, date 30-40 part, hawthorn 3-40 part, fruit of Chinese wolfberry 0.4-0.6 part, ant 0.4-0.6 part; Described health food is tablet, granule, capsule or the oral liquid that contains pharmaceutic adjuvant;
Preparation process is as follows:
The Radix Astragali is cleaned, date, hawthorn are cleaned stoning, the Radix Astragali decocts 2-5 time, adds the water that 10-30 doubly measures at every turn, decocts 1-2 hour, merge extract, in extract, add the fruit of Chinese wolfberry, date, hawthorn and ant, decocted 30-120 minute, filter, add pharmaceutic adjuvant, make following different dosage form respectively: tablet, granule, capsule or oral liquid.
2. health food according to claim 1 is characterized in that, in weight portion, its raw material consists of: Radix Astragali 15-25 part, date 35-40 part, hawthorn 20-30 part, fruit of Chinese wolfberry 0.45-0.55 part, ant 0.45-0.55 part.
3. health food according to claim 1 is characterized in that, in weight portion, its raw material consists of: 20 parts of the Radixs Astragali, 35 parts in date, 30 parts of hawthorn, 0.5 part of the fruit of Chinese wolfberry, 0.5 part of ant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103848305A CN102406172B (en) | 2011-11-28 | 2011-11-28 | Health-care food for enhancing immunity and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103848305A CN102406172B (en) | 2011-11-28 | 2011-11-28 | Health-care food for enhancing immunity and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102406172A CN102406172A (en) | 2012-04-11 |
| CN102406172B true CN102406172B (en) | 2013-09-11 |
Family
ID=45908739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103848305A Active CN102406172B (en) | 2011-11-28 | 2011-11-28 | Health-care food for enhancing immunity and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102406172B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940287A (en) * | 2012-11-07 | 2013-02-27 | 哈尔滨医科大学 | Beverage capable of enhancing immunity and preparation method thereof |
| CN107568740A (en) * | 2017-09-07 | 2018-01-12 | 哈尔滨医科大学 | A kind of health-care food composition, its preparation method and the purposes in terms of strengthen immunity |
-
2011
- 2011-11-28 CN CN2011103848305A patent/CN102406172B/en active Active
Non-Patent Citations (6)
| Title |
|---|
| 卓斌等.山楂的药理作用研究.《亚太传统医药》.2009,(第03期), |
| 姜枣、芪杞枣的开发与研制;高平;《吕梁高等专科学校学报》;20060610(第02期);9-10 * |
| 山楂的药理作用研究;卓斌等;《亚太传统医药》;20090315(第03期);18-19 * |
| 蚂蚁对断乳仔兔免疫系统影响的试验研究;赵权等;《经济动物学报》;19990830(第03期);5-8 * |
| 赵权等.蚂蚁对断乳仔兔免疫系统影响的试验研究.《经济动物学报》.1999,(第03期), |
| 高平.姜枣、芪杞枣的开发与研制.《吕梁高等专科学校学报》.2006,(第02期), |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102406172A (en) | 2012-04-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102613555B (en) | Maca powder and ginseng composition and preparation method thereof | |
| CN102204678B (en) | Instant cubilose powder with efficacy of immunity enhancement and preparation method thereof | |
| CN102526477B (en) | Medicine composition for enhancing immunities as well as preparation method and application thereof | |
| CN101856112A (en) | Health food for enhancing immune function of human body and preparation method thereof | |
| CN103585400A (en) | Composition having immunity enhancing and fatigue alleviating effects, and preparation method thereof | |
| CN104127577A (en) | Chinese medicine capsule for relieving physical fatigue and preparation method of Chinese medicine capsule | |
| CN106214845A (en) | A kind of pharmaceutical composition with health care and preparation method thereof | |
| CN105055855B (en) | With Chinese medicine composition for improving sleep, strengthen immunity effect and its preparation method and application | |
| CN102429150A (en) | Vitamin C chewable tablets for enhancing human immunity | |
| CN101032599B (en) | Healthy solution for conditioning skin and losing weight | |
| CN102754833B (en) | Healthcare product with function of enhancing immunity and preparation method thereof | |
| CN108813610A (en) | A kind of saussurea involucrata composition and its application for improving immunity | |
| CN102406172B (en) | Health-care food for enhancing immunity and preparation method thereof | |
| CN105535425B (en) | A kind of Chinese medicine composition with strengthen immunity effect and preparation method thereof and purposes | |
| CN107156830A (en) | A kind of composition of strengthen immunity, its preparation method and application | |
| CN103690573B (en) | A kind of natural drug composition for strengthen immunity and preparation method thereof | |
| CN106728387B (en) | Compound medicine with function of promoting immunity and preparation method thereof | |
| CN105727253A (en) | Composition for protecting liver, clearing away toxic materials, preventing and treating liver and related metabolic diseases and preparation method thereof | |
| CN107951992A (en) | A kind of Chinese medicine composition for strengthen immunity and preparation method thereof | |
| CN104189038B (en) | Chinese medicine preparation for nursing one's health female pathology health and immunologic function | |
| CN106214844A (en) | A kind of compositions with fat-reducing and immunoregulation effect and preparation method thereof | |
| CN102973725A (en) | Lentinan capsules | |
| CN102920847A (en) | Composition for strengthening immunity and preparation and application thereof | |
| CN105815786A (en) | Tablets prepared from honeysuckle flowers, wild chrysanthemum flowers and rhizoma polygonati and preparation method of tablets | |
| CN102258715A (en) | Honeysuckle wine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HEILONGJIANG TIANYI PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: HARBIN MEDICAL UNIVERSITY Effective date: 20150430 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 150081 HARBIN, HEILONGJIANG PROVINCE TO: 163316 DAQING, HEILONGJIANG PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20150430 Address after: 163316 Jianshe Road high tech Zone, Heilongjiang, No. 245, Patentee after: Heilongjiang Tianyi Pharmaceutical Co., Ltd. Address before: 150081 Nangang City, Heilongjiang Province Health Road, No. 157, Patentee before: Harbin Medical University |