CN102940287A - Beverage capable of enhancing immunity and preparation method thereof - Google Patents
Beverage capable of enhancing immunity and preparation method thereof Download PDFInfo
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Abstract
本发明涉及一种增强免疫力的饮料及其制备方法，其含有黄芪10-30份，大枣20-60份，山楂20-60份，枸杞5-15份，液体木糖醇5-10份，安赛蜜1-10份，果胶1-5份，三氯蔗糖0.1-1份，纯净水10000-50000份。取10-30重量份黄芪、20-60重量份大枣、20-60重量份山楂，清洗去杂后煎煮，每次煎煮加10-30倍重量的水，煎煮0.5-3小时，共煎煮2-5次，每次煎煮后取滤液，第二次煎煮最后30分钟加入5-15重量份枸杞子，合并滤液；向前述滤液中加入5-10重量份木糖醇，1-10重量份安赛蜜，1-5份重量份果胶和0.1-1份三氯蔗糖，加入适量纯净水后，杀菌，灌装即得。本发明饮料具有增强免疫提高机体抵抗力的功效。The invention relates to a drink for enhancing immunity and a preparation method thereof, which contains 10-30 parts of astragalus, 20-60 parts of jujube, 20-60 parts of hawthorn, 5-15 parts of wolfberry and 5-10 parts of liquid xylitol , 1-10 parts of acesulfame potassium, 1-5 parts of pectin, 0.1-1 part of sucralose, 10000-50000 parts of purified water. Take 10-30 parts by weight of astragalus, 20-60 parts by weight of jujube, and 20-60 parts by weight of hawthorn, wash and remove impurities, and then decoct. Add 10-30 times the weight of water for each decoction, and decoct for 0.5-3 hours. Decoct 2-5 times in total, take the filtrate after decocting each time, add 5-15 parts by weight of medlar in the last 30 minutes of the second decoction, and combine the filtrate; add 5-10 parts by weight of xylitol to the aforementioned filtrate, 1-10 parts by weight of acesulfame-K, 1-5 parts by weight of pectin and 0.1-1 part by weight of sucralose are added to an appropriate amount of purified water, sterilized and filled to obtain the product. The beverage of the invention has the effect of enhancing immunity and improving body resistance.
Description
Technical field
The present invention relates to a kind of health drink, particularly relate to a kind of beverage for strengthening immunity of organisms and preparation method thereof.
Background technology
The development of modern science and technology makes people's work rhythm more and more faster, and pressure is also increasing, and the thing followed causes that increasing people's immunity reduces, even more people are in sub-health state.Exploitation more can improve body immunity, and the medicine, health products even the food that improve people's sub-health state seem more and more important and urgent.Publication number be CN101263909A Patent Application Publication a kind of health drink that strengthens immunity function, contain the components such as glossy ganoderma, the fruit of Chinese wolfberry, Poria cocos, the fruit of Chinese magnoliavine, Radix Angelicae Sinensis, has enhancing immunity, the plurality of health care functions such as anti-ageing, anti-oxidant, antitumor, long-term drinking can reach good health-care effect.But this method for preparing health drink is complicated, the extraction step that needs water extraction and alcohol extracting to combine.Wear Jingjing etc. and (wear Jingjing, Zhang Yuetian etc. strengthen the development .[J of immunity, physical-fatigue-relieving health beverage]. food development and machinery, 2008,3:98-100.) take American Ginseng as the monarch drug in a prescription Radix Astragali as ministerial drug, be equipped with syrup, honey, citric acid, vitamin etc. and prepare a kind of health drink that strengthens immunity, alleviating physical fatigue.Have the effect that improves deficiency of vital energy patient immune function, improves deficiency of vital energy symptom, the low and fatiguability crowd of suitable immunity is not suitable for pregnant woman and drinking of children.
Summary of the invention
The technical problem to be solved in the present invention provides and a kind ofly can strengthen beverage of immunity and preparation method thereof.
For reaching above-mentioned purpose, a kind of beverage that strengthens immunity of the present invention, it contains the raw material components of following weight portion: Radix Astragali 10-30 part, date 20-60 part, hawthorn 20-60 part, matrimony vine 5-15 part, liquid xylitol 5-10 part, acesulfame potassium 1-10 part, pectin 1-5 part, Sucralose 0.1-1 part, 10000-50000 part pure water.
Preferably, contain the raw material components of following weight portion in the described beverage: 20 parts of the Radixs Astragali, 40 parts in date, 30 parts of hawthorn, 10 parts of matrimony vines, 8 parts of liquid xylitols, 6 parts of acesulfame potassiums, 3 parts of pectin, 0.5 part of Sucralose, 30000 parts of pure water.
The present invention prepares the method for the beverage of above-mentioned enhancing immunity, may further comprise the steps:
Get the Radix Astragali, date, hawthorn, decoct after the cleaning impurity elimination, each water that adds 10-30 times of weight that decocts decocted 0.5-3 hour, decocted 2-5 time altogether, got filtrate after each the decoction, decocted the second time to add the fruit of Chinese wolfberry, merging filtrate in last 30 minutes;
In aforementioned filtrate, add xylitol, acesulfame potassium, pectin and Sucralose, add pure water after, sterilization, can and get final product.
Raw material reasonable recipe of the present invention: the void of the person, universal uneven, nothing more than qi and blood two ends.The sweet temperature of the Radix Astragali, all void of energy tonifying five zang organs, the energy warming with drugs of thick nature is to mend the deficiency of shape; Date is sweet flat, and beneficial the five internal organs are mended vital essence, born fluid, and regulating spleen and stomach, as a means of the change source, the energy tonifying with drugs of thick flavor, with the deficiency of benefit essence, the two is monarch drug in a prescription altogether.Minister is with hawthorn, and its flavor is little sweet to acid, and the red flesh-coloured Huang of skin so to be apt to into blood system be the key medicine of Removing Blood Stasis, and doublely enter gas and divides out obstruction of the circulation of vital energy phlegm knot, the qi and blood punching and, ten thousand diseases are not given birth to; Its flavor is sour and little sweet, can subsidize sour juice in the stomach, gathers so can digest diet, with tonic phase 5, can make its tonify without causing stagnation.The sweet cold profit of matrimony vine is the medicine of flat benefit, energy kidney tonifying moistening lung, and production of sperm benefit gas helps date to mend the merit of flavor benefit essence.All medicine compatibilities, the five internal organs must be mended, qi and blood punching and, all diseases are from removing.
The beverage of enhancing immunity of the present invention has the enhancing immunologic function, improves the effect of Abwehrkraft des Koepers.
The specific embodiment
Below in conjunction with embodiment and test data, be described in more detail with other technical characterictic and advantage the present invention is above-mentioned.
Embodiment 1
Get the 10g Radix Astragali, 30g date, 30g hawthorn, add the water (being 1400g) of 20 times of amounts at every turn, decocted 1 hour, decoct altogether twice.Decoct for the second time the last 30 minutes adding 5g fruits of Chinese wolfberry, merge filtrate twice.
In aforementioned filtrate, add xylitol 5g, acesulfame potassium 5g, pectin 2g and Sucralose 0.1g, behind the adding 10000g pure water, sterilization is filled into and namely gets this product in the 500mL bottle.
Embodiment 2
Get the 20g Radix Astragali, 20g date, 40g hawthorn, add the water (being 800g) of 10 times of amounts at every turn, decocted 1 hour, decoct altogether three times.Decoct for the second time the last 30 minutes adding 5g fruits of Chinese wolfberry, merge three times filtrate.
In aforementioned filtrate, add xylitol 6g, acesulfame potassium 8g, pectin 4g and Sucralose 0.5g, behind the adding 10000g pure water, sterilization is filled into and namely gets this product in the 500mL bottle.
Embodiment 3
Get the 30g Radix Astragali, 60g date, 60g hawthorn, add the water (being 4500g) of 30 times of amounts at every turn, decocted 3 hours, decoct altogether five times.Decoct for the second time the last 30 minutes adding 15g fruits of Chinese wolfberry, merge five times filtrate.
In aforementioned filtrate, add xylitol 10g, acesulfame potassium 10g, pectin 5g and Sucralose 1.0g, behind the adding 50000g pure water, sterilization is filled into and namely gets this product in the 500mL bottle.
Embodiment 4
Get the 20g Radix Astragali, 40g date, 30g hawthorn, add the water (being 1800g) of 20 times of amounts at every turn, decocted 2 hours, decoct altogether three times.Decoct for the second time the last 30 minutes adding 10g fruits of Chinese wolfberry, merge three times filtrate.
In aforementioned filtrate, add liquid xylitol 8g, acesulfame potassium 6g, pectin 3g and Sucralose 0.5g, behind the adding 30000g pure water, sterilization is filled into and namely gets this product in the 500mL bottle.
Test example strengthens the immunity requirement of experiment according to health food and estimates effect of the present invention
1. animal used as test and grouping
100 of healthy SPF level ICR mouse are female.Select 24 of Healthy female mouse, body weight 18～22g is divided into 2 groups at random, 12 every group, carries out that carbon is cleaned up experiment and animal is dirty/the body ratio measurement.24 of Healthy female mouse, body weight 18～22g is divided into 2 groups at random, 12 every group, carries out HD50 value (HC
50) measure and the antibody-producting cell detection.24 of Healthy female mouse, body weight 18～22g is divided into 2 groups at random, 12 every group, carries out delayed allergy experiment and Turnover of Mouse Peritoneal Macrophages and engulfs the chicken red blood cell test.24 of Healthy female mouse, body weight 18～22g is divided into 2 groups at random, 12 every group, carries out mouse spleen lymphocyte transformation experiment and NK cytoactive detection that ConA induces.
2. given the test agent and dosage
Product (sample 1) with embodiment 1-4 obtains gave mouse 60 days by the mode of independently drinking every day.
3. test method and result
3.1 the mouse spleen lymphocyte conversion test that ConA induces and NK cytoactive detection.
3.1.1 splenocyte suspension preparation
The aseptic spleen of getting of animal places the plate that fills an amount of aseptic Hanks liquid, gently spleen is torn up with tweezers, makes the individual cells suspension.Filter through 200 eye mesh screens, wash 2 times with Hanks liquid, each centrifugal 10min (1000r/min) abandons supernatant, cytoplasm is upspring, added the 0.5ml aqua sterilisa 20 seconds, splitting erythrocyte, and then add 0.5ml2 Hanks liquid and 8mlHanks liquid doubly, and 1000r/min, 10min is centrifugal.With the 1ml1640 complete culture solution cell is suspended at last, with the blue dyeing counting viable count (more than 95%) of platform phenol.
3.1.2 batch lymphocyte transformation test (mtt assay)
The splenocyte stoste of getting above-mentioned preparation is an amount of, and making cell concentration with the dilution of 1640 complete culture solutions is 2 * 10
6Individual/ml.Cell suspension divides two holes to add in 24 well culture plates after will diluting, every hole 1ml, and a hole adds 75 μ l ConA liquid (being equivalent to 5 μ g/ml), and 5%CO is put in contrast in another hole
2, cultivate 72h for 37 ℃.Cultivate and finish front 4h, every hole sucks supernatant 0.7m1 gently, adds the RPMI RPMI-1640 that 0.7m1 does not contain calf serum, add simultaneously MTT (5mg/ml) and dissolve fully, then divide to install in 96 well culture plates, 3 parallel holes are done in each hole, use ELIASA, measure the OD value with 570nm.The result is as follows:
The impact that table 1 sample 1 transforms mouse spleen lymphocyte
By as seen from Table 1, mouse was independently drunk sample after 160 days, and learn by statistics and process, experimental mice SPL transformation function and negative control group comparison, there was no significant difference (P〉0.05), namely sample 1 does not affect the mouse spleen lymphocyte transformation function.
3.1.3 NK cytoactive detection (LDH determination method)
It is an amount of that other gets above-mentioned splenocyte stoste, and with the dilution of 1640 complete culture solutions, making cell concentration is 2 * 10
7Individual/ml action effect cell concentration, adjust accordingly again target cell (YAC-1) concentration, making effect target ratio is 50: 1.Get each 100 μ l of target cell and effector cell, the maximum release aperture of target cell adds target cell and each 100 μ l of 1%NP40, above-mentioned every three parallel holes of all establishing, 37 ℃, 5%CO
2Cultivate 4h in the incubator, with 96 orifice plates with 1500rpm centrifugal 5 minutes, every hole was drawn supernatant 100 μ l and is put in the ELISA Plate, add LDH matrix liquid 100 μ l, reacted 10 minutes, then every hole adds the HCl solution 30 μ l cessation reactions of 1mol, surveys the OD value at ELIASA 490nm place and calculates the NK cytoactive.
NK%=(reacting hole OD-Spontaneous release hole OD)/(maximum release aperture OD-Spontaneous release hole OD) * 100%
The result is as follows:
Table 2 sample 1 is on the impact of NK cells in mice activity
By as seen from Table 1, mouse was independently drunk sample after 160 days, and learn by statistics and process, the NK cytoactive of experimental mice and negative control group comparison there was no significant difference (P〉0.05).Be that sample 1 is active in impact on NK cells in mice.
3.2 delayed allergy and Turnover of Mouse Peritoneal Macrophages are engulfed chicken red blood cell experiment (half intracorporal method)
3.2.1 delayed allergy (the sufficient sole of the foot thickens method)
Every mouse of immune three treated animals through lumbar injection 2%(V/V, is prepared with physiological saline) hematocrit SRBC(2000rpm, 10 minutes) 0.2ml, after the sensitization 4 days, measure left back sufficient sole of the foot section thickness, same position is measured three times, averages.Then at measuring point hypodermic injection 20%(V/V, prepare with physiological saline) hematocrit SRBC 20 μ l, measured left back sufficient sole of the foot section thickness in rear 24 hours in injection, represent the degree of DTH with the difference (swelling degree of the paw) of sufficient sole of the foot thickness before and after attacking.The result is as follows:
Table 3 sample 1 is on the impact of mouse delayed allergy (DTH)
Annotate: * and negative control group relatively have significant difference P＜0.05
By as seen from Table 3, mouse was independently drunk sample after 160 days, learned by statistics and processed, and its swelling degree of the paw (the sufficient sole of the foot thickens) is behind injection SRBC 24h, significant difference (P＜0.05) is relatively arranged between experimental group and negative control group, and namely sample 1 can strengthen the delayed allergy of mouse.
3.2.2 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (half intracorporal method)
Finish rear 2 days in above-mentioned delayed allergy, its left back sufficient sole of the foot no longer after the swelling, carries out Turnover of Mouse Peritoneal Macrophages with this treated animal and engulfs the chicken red blood cell experiment.
3.2.2.1 chicken erythrocyte suspension preparation
Get the conical flask that chicken blood places bead, fully shake towards a direction, with defiber.With physiological saline washing 2～3 times, centrifugal (2000r/min, 10min) removes supernatant, is made into the chicken erythrocyte suspension of 20% (v/v) with physiological saline.
3.2.2.2 detection of phagocytic function
Every mouse lumbar injection 20% chicken erythrocyte suspension 1ml, interval 30min, animal is put to death in the cervical vertebra dislocation, it is faced upward the position be fixed on the mouse plate, abdominal skin is cut off in the center, through Intraperitoneal injection physiological saline 2ml, rotate mouse plate 1min, then sucking-off abdominal cavity washing lotion 1m, average mark drip in 2 and carry on the glass, put into the enamel box that is lined with wet husky cloth, 37 ℃ of incubator temperature of dislocation 30min, incubate completely, rinsing in physiological saline is to remove not paster cell, dry, fix with 1:1 acetone methanol solution, 4% (v/v) Giemsa-phosphate buffer dyeing 3min dries with the distilled water rinsing again.
The result represents the phagocytic function of mouse macrophage with phagocytic percentage or phagocytic index, and 100 macrophages of counting are calculated as follows phagocytic percentage and phagocytic index under the oily mirror.
Phagocytic rate %=(engulfing the macrophage number of chicken red blood cell/100 macrophages) * 100%
Chicken red blood cell sum/100 macrophages that phagocytic index=quilt is engulfed
The result is as follows:
Table 4 sample 1 is engulfed erythrocytic impact to mouse macrophage
Annotate: * and negative control group relatively have significant difference P＜0.05
By as seen from Table 4, mouse was independently drunk sample after 160 days, learned by statistics and processed, and phagocytic rate is carried out homogeneity test of variance, and heterogeneity of variance carries out change of variable
P is phagocytic rate in the formula, decimally expression.For initial data carry out after the change of variable and variance neat, the comparative result that carries out.The phagocytic index of experimental group and negative control group relatively have significant difference, P＜0.05.It is the phagocytic function that sample 1 can strengthen Turnover of Mouse Peritoneal Macrophages.
3.3 the mensuration of HD50 value and antibody-producting cell detect
3.3.1 animal immune carried out animal immune in front 4～5 days in the experiment end.Get the sheep blood of defiber, with physiological saline washing 3 times, each centrifugal 2000r/min, 10min.Hematocrit SRBC is made into the cell suspension of 2% (v/v), every mouse lumbar injection 0.2mL with physiological saline.
3.3.2 the mensuration of HD50 value
Behind the animal immune 4～5 days, extract eyeball and get blood in centrifuge tube, placed about 1 hour, solidification blood and tube wall are peeled off, serum is fully separated out, centrifugal 10 minutes of 2000rpm collects serum., get 1ml and put in vitro 250 times of serum dilutions with the SA buffer solution, add successively 10%(V/V, with the preparation of SA buffer solution) hematocrit SRBC 0.5ml, complement 1ml(presses the 1:10 dilution with the SA buffer solution).Other establishes the not control tube of increase serum (replacing with the SA buffer solution).Put in 37 ℃ of waters bath with thermostatic control insulation after 30 minutes, the ice bath cessation reaction.Centrifugal 10 minutes of 2000rpm, get supernatant 1ml, add Dou Shi reagent 3ml, get simultaneously 10%(V/V, with SA buffer solution preparation) hematocrit SRBC 0.25ml, add Dou Shi reagent to 4ml in another test tube, abundant mixing, place after 10 minutes, sentence control tube in 540nm and make blank, measure respectively and respectively manage OD value.The amount of hemolysin is with HD50 value (HC
50) expression, be calculated as follows.
Sample HC
50OD value * extension rate during=sample OD value/SRBC HD50
The result is as follows:
1 pair of mouse HD50 of table 5 sample value (HC
50) impact
Annotate: * and negative control group relatively have significant difference P＜0.05
By as seen from Table 5, mouse was independently drunk sample after 160 days, and the HD50 value of experimental group and negative control group relatively have significant difference (P＜0.05), show that sample 1 can improve mouse HD50 value.
3.3.3 antibody-producting cell detects (Jerne improves slide method)
3.3.3.1 splenocyte suspension preparation (the same 3.1.1)
3.3.3.2 the mensuration of plaque
After top layer culture medium (the 1g agarose adds distilled water to 100ml) heating for dissolving, put 45～50 ℃ of water bath heat preservations, with equivalent pH 7.2～7.4, the Hanks liquid of 2 times of concentration mixes, the packing small test tube, every pipe 0.5ml adds 50 μ l 10%SRBC (v/v again in pipe, with the preparation of SA liquid), 20 μ l splenocyte suspensions (5 * 10
6Individual/ml), rapid mixing, be poured on the slide of brushing the agarose thin layer, after agar solidifies, the slide level buckled be placed on the horse, put into CO2gas incubator incubation 1～1.5h, then the complement (1:8) with the dilution of SA buffer solution joins in the slide frame groove, after continuing incubation 1～1.5h, counting hemolysis plaque number.The result is as follows:
Table 6 sample 1 is on the impact of mouse antibodies cellulation
By as seen from Table 9, mouse was independently drunk sample after 160 days, and experimental group hemolysis plaque number and negative control group be there was no significant difference (P〉0.05) relatively, and interpret sample 1 can not strengthen mouse antibodies cellulation function.
3.4 mouse carbon is cleaned up experiment
Mouse treats that by the india ink that 0.1ml/10g body weight tail vein injection 1:3.5 doubly dilutes prepared Chinese ink injects immediately timing.Injected behind the prepared Chinese ink 2,10 minutes, and got blood 20 μ l from the angular vein clump respectively, and it is added to 2mLNa
2CO
3In the solution.The mouse rear cervical vertebra dislocation of weighing is put to death, get liver, spleen and thymus gland and remove most manadesma, blot the organ surface blood stains with filter paper, weigh.
With 722 spectrophotometers at 600nm wavelength place's photometry density value (OD), with Na
2CO
3Solution is made blank.Be calculated as follows phagocytic index α:
K＝(lgOD
1?lgOD
2)/(t
2－t
1)
α=body weight ÷ (liver weight+spleen is heavy) * √ K)
The result is as follows:
Table 7 sample 1 is cleaned up the impact of function on mouse carbon
Annotate: * and negative control group relatively have significant difference P＜0.05.
By as seen from Table 7, mouse was independently drunk sample after 160 days, learned by statistics and processed, and experimental group phagocytic index and negative control group relatively have significant difference (P＜0.05), and namely sample 1 can increase mouse carbon and cleans up function.
4 brief summaries
Mouse was independently drunk sample after 160 days, carried out the check of four classes and amounted to 7 tests, namely (antibody-producting cell detects (improvement slide method) and HD50 value (HC for cellular immune function (the mouse spleen lymphocyte conversion test (mtt assay) that ConA induces and delayed allergy (the sufficient sole of the foot thickens method)), humoral immune function
50) mensuration), the detection of the detection of monocytes/macrophages phagocytic function (mouse carbon clean up experiment and Turnover of Mouse Peritoneal Macrophages engulf chicken red blood cell test) and NK cytoactive.The result shows that present embodiment product (being sample 1) can obviously improve the HD50 value of mouse, and the carbon that can strengthen the delayed allergy of mouse and can strengthen mouse is cleaned up the phagocytic function of function and peritoneal macrophage.According to the criterion in " function of health food is learned assessment process and the method for inspection ", think that present embodiment product (being sample 1) has immunoregulation effect, namely beverage of the present invention has the effect that strengthens immunity, improves Abwehrkraft des Koepers.
Above-described embodiment is described preferred embodiment of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (3)
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103932176A (en) * | 2014-04-03 | 2014-07-23 | 哈尔滨医科大学 | Health-care food composition for assisting to lower blood flat, as well as preparation method and application of composition |
| CN104126690A (en) * | 2014-08-14 | 2014-11-05 | 张国勇 | Tea for nourishing heart and protecting liver |
| CN106666288A (en) * | 2017-01-12 | 2017-05-17 | 山东奇华顿食品科技有限公司 | Kiwi fruit drink and preparation process thereof |
| CN107568740A (en) * | 2017-09-07 | 2018-01-12 | 哈尔滨医科大学 | A kind of health-care food composition, its preparation method and the purposes in terms of strengthen immunity |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608646A (en) * | 2003-10-17 | 2005-04-27 | 天津药物研究院 | Wolfberry fruit-astragalus root oral liquid and its prepn process |
| CN1951228A (en) * | 2005-11-27 | 2007-04-25 | 王可剑 | A replenishing drink |
| CN101099571A (en) * | 2007-08-03 | 2008-01-09 | 司家勇 | Health-care food for strengthening immunity and its preparing method |
| KR100856330B1 (en) * | 2007-05-07 | 2008-09-03 | 허만복 | Fruit fruit drink prepared by adding antler extract and its manufacturing method |
| CN102406172A (en) * | 2011-11-28 | 2012-04-11 | 哈尔滨医科大学 | Health food for enhancing immunity and preparation method thereof |
-
2012
- 2012-11-07 CN CN2012104409051A patent/CN102940287A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608646A (en) * | 2003-10-17 | 2005-04-27 | 天津药物研究院 | Wolfberry fruit-astragalus root oral liquid and its prepn process |
| CN1951228A (en) * | 2005-11-27 | 2007-04-25 | 王可剑 | A replenishing drink |
| KR100856330B1 (en) * | 2007-05-07 | 2008-09-03 | 허만복 | Fruit fruit drink prepared by adding antler extract and its manufacturing method |
| CN101099571A (en) * | 2007-08-03 | 2008-01-09 | 司家勇 | Health-care food for strengthening immunity and its preparing method |
| CN102406172A (en) * | 2011-11-28 | 2012-04-11 | 哈尔滨医科大学 | Health food for enhancing immunity and preparation method thereof |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103932176A (en) * | 2014-04-03 | 2014-07-23 | 哈尔滨医科大学 | Health-care food composition for assisting to lower blood flat, as well as preparation method and application of composition |
| CN103932176B (en) * | 2014-04-03 | 2015-06-24 | 哈尔滨医科大学 | A health food composition with the function of assisting in lowering blood fat, its preparation method and application |
| CN104126690A (en) * | 2014-08-14 | 2014-11-05 | 张国勇 | Tea for nourishing heart and protecting liver |
| CN106666288A (en) * | 2017-01-12 | 2017-05-17 | 山东奇华顿食品科技有限公司 | Kiwi fruit drink and preparation process thereof |
| CN107568740A (en) * | 2017-09-07 | 2018-01-12 | 哈尔滨医科大学 | A kind of health-care food composition, its preparation method and the purposes in terms of strengthen immunity |
| CN108720025A (en) * | 2018-06-08 | 2018-11-02 | 唐飞 | 25-hydroxy-vitamin D3Application in food |
| CN109090602A (en) * | 2018-08-07 | 2018-12-28 | 北京筑元吉康医药科技有限责任公司 | A kind of health food of strengthen immunity and its application |
| CN110540920A (en) * | 2019-08-21 | 2019-12-06 | 贵州仙草生物科技有限公司 | A kind of dendrobium nobile wine and preparation method thereof |
| CN113575806A (en) * | 2021-08-05 | 2021-11-02 | 横琴绿环科技有限公司 | Turmeric compound beverage and preparation method thereof |
| CN115895815A (en) * | 2022-11-15 | 2023-04-04 | 云南滇药医药健康产业有限公司 | Herbal fermented cordial and preparation method thereof |
| CN115895815B (en) * | 2022-11-15 | 2024-02-02 | 云南滇药医药健康产业有限公司 | Herbal fermented wine and preparation method thereof |
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Application publication date: 20130227 |