CN115088849A - Compound oligomeric donkey-hide gelatin peptide product for qi benefiting and blood nourishing comprehensive conditioning and preparation method thereof - Google Patents
Compound oligomeric donkey-hide gelatin peptide product for qi benefiting and blood nourishing comprehensive conditioning and preparation method thereof Download PDFInfo
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- CN115088849A CN115088849A CN202210834550.8A CN202210834550A CN115088849A CN 115088849 A CN115088849 A CN 115088849A CN 202210834550 A CN202210834550 A CN 202210834550A CN 115088849 A CN115088849 A CN 115088849A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
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Abstract
The invention relates to the field of food processing, and discloses a composite oligosaccharide donkey-hide gelatin peptide product for tonifying qi and nourishing blood for comprehensive conditioning and a preparation method thereof, wherein the composite oligosaccharide donkey-hide gelatin peptide product comprises the following raw and auxiliary materials in parts by weight: 50-70 parts of donkey-hide gelatin peptide, 25-50 parts of ginseng oligopeptide, 25-50 parts of walnut oligopeptide, 7-10 parts of red date, 3-5 parts of astragalus membranaceus, 3-5 parts of medlar, 1-2 parts of bighead atractylodes rhizome, 8-18 parts of resistant dextrin and 1-10 parts of L-glutamine. Through a large number of experiments and researches, the traditional Chinese medicine composition has the effects of enriching blood and nourishing blood, is suitable for deficiency of both qi and blood, palpitation, amnesia, insomnia, dizziness and the like, improves the self hematopoietic function by improving the internal secretion and metabolism of a human body, has small side effect and definite curative effect, and avoids the problem that the existing western medicine treats symptoms and does not treat the root causes. And has good taste, no toxic or side effect, safety and easy popularization.
Description
Technical Field
The invention belongs to the field of food and food processing, and particularly relates to a composite oligosaccharide donkey-hide gelatin peptide product for comprehensively conditioning qi and blood and a preparation method thereof.
Background
In the process of human growth, blood is the important material basis of human life activities, contains various nutrient substances required by human body, plays a role in nutrition for all viscera tissues of the whole body, continuously produces blood in the life of a human, continuously supplies nutrition for all viscera tissues of the human body, and is easy to cause blood deficiency if the physical strength is greatly consumed and the nutrition is insufficient. The blood deficiency is characterized by pale or sallow complexion, pale lip and nail, dizziness, insomnia, amnesia, palpitation, hypomenorrhea or amenorrhea, pale tongue and thin pulse, the blood deficiency causes symptoms such as weak body, insufficient blood and qi, frequent mental fatigue, soreness and weakness of waist and feet, and the deficiency anemia can cause symptoms such as dizziness, tinnitus, headache, insomnia, dreaminess, hypomnesis, inattention, paleness, dyspnea, dyspepsia, oliguria, anuresis, acute renal failure and the like. In addition, qi deficiency, essence deficiency, blood stasis, etc. can also lead to blood deficiency.
The colla Corii Asini is solid glue prepared by decocting dried or fresh skin of Equus asinus L of Equidae, and concentrating. Sweet and neutral in nature and flavor, enters lung, liver and kidney meridians, has the functions of enriching blood, stopping bleeding, nourishing yin and moistening dryness, is mainly used for treating blood deficiency, and can be used for auxiliary treatment of tumors. Donkey-hide gelatin mainly contains collagen, but digestion and absorption of the collagen require protease to participate in degradation, and patients with indications are often weak in spleen and stomach or have gastrointestinal reactions, and taking donkey-hide gelatin not only causes burden on the digestive system of patients, but also cannot give full play to the drug effect.
The micromolecule donkey-hide gelatin peptide obtained by processing donkey-hide gelatin by adopting the modern biological enzyme digestion technology can effectively overcome the problems. The donkey-hide gelatin peptide has the following characteristics: 1. the donkey-hide gelatin peptide has obvious blood enriching effect on iron-deficiency anemia. It has effects in increasing erythrocyte and hemoglobin, and promoting hematopoiesis; can increase leukocyte and platelet and blood oxygen content. 2. The colla Corii Asini peptide can increase serum calcium content, regulate calcium balance in human body, and improve anoxia and fatigue resistance. 3. The colla Corii Asini peptide has effects of invigorating qi, nourishing blood, relieving fatigue, resisting shock, resisting radiation, resisting muscle atrophy, resisting cold, improving immunity, nourishing yin, invigorating kidney, strengthening tendons and bones, inducing diuresis, and relieving swelling. Qi and blood are the basic substances that make up the human body, and are related to the operation of the zang-fu organs and the health of the body, and they have the function of communicating with each zang-fu organ, so that the qi and blood disorder of one zang-fu organ can not only cause the pathological changes of the zang-fu organ, but also cause the qi and blood disorder of other zang-fu organs, and even the qi and blood disorder of the whole body. For people with blood deficiency, food nourishing is the best method, but the simple food nourishing has slow effect and insignificant effect, and the compound oligomeric donkey-hide gelatin peptide product which is prepared by selecting certain food to be matched with traditional Chinese medicines to enrich the blood and scientifically processing is easy to absorb and can play the roles of quickly enriching the blood and nourishing the liver, nourishing the heart and benefiting the spleen.
Disclosure of Invention
Based on the above, one of the purposes of the invention is to provide a composite donkey-hide gelatin peptide product for qi-tonifying and blood-nourishing comprehensive conditioning, which can improve endocrine and metabolism of a human body and improve the hematopoietic function of the human body, is suitable for diseases of qi deficiency and blood deficiency, particularly qi-blood deficiency, and has small side effect and definite curative effect.
The invention realizes the purpose through the following technical scheme: the composite donkey-hide gelatin peptide product for benefiting qi and nourishing blood for comprehensive conditioning comprises the following raw and auxiliary materials in parts by weight: 50-70 parts of donkey-hide gelatin peptide, 25-50 parts of ginseng oligopeptide, 25-50 parts of walnut oligopeptide, 7-10 parts of red date, 3-5 parts of astragalus membranaceus, 3-5 parts of medlar, 1-2 parts of bighead atractylodes rhizome, 8-18 parts of resistant dextrin and 1-10 parts of L-glutamine.
Further, the preparation method of the donkey-hide gelatin peptide comprises the following steps:
(1) dissolving: taking donkey-hide gelatin, carrying out superfine grinding, sieving with a 100-mesh and 500-mesh sieve, placing the donkey-hide gelatin in an enzymolysis reaction tank, adding 8-12 times of water, starting a stirring paddle, stirring for 0.5-1.0 h to enable the donkey-hide gelatin to be uniformly suspended, introducing steam into an interlayer of the tank body, heating and boiling for 30min, closing the steam, and introducing cooling water to cool the temperature in the tank to 50-55 ℃;
(2) enzymolysis: adding a high-efficiency compound enzyme preparation with the dosage of 0.05-0.15% of the weight of the raw materials into the solution obtained in the step (1), uniformly mixing, and carrying out enzymolysis for 0.5-2 hours under natural conditions of no pH value adjustment and no temperature control to obtain a donkey-hide gelatin peptide solution; the high-efficiency compound enzyme preparation consists of 30-40 wt% of compound protease, 25-30 wt% of flavourzyme, 25-30 wt% of alkaline protease, 7-8 wt% of trypsin and 2-3 wt% of enzyme activator, wherein the enzyme activator contains a reducing agent and divalent metal ions; the reducing agent is dithiothreitol and/or beta-mercaptoethanol; the molar ratio of the reducing agent to the divalent metal ions in the enzyme activator is 1 (0.5-2); the divalent metal ions are a mixture of Mg2+, Zn2+ and Ca2 +;
(4) filtering with an inorganic nanofiltration ceramic membrane: filtering the donkey-hide gelatin peptide solution by adopting an inorganic nanofiltration ceramic membrane with the molecular weight cutoff of 800Da, wherein the obtained permeate is a high-purity donkey-hide gelatin peptide solution;
(5) high-temperature high-pressure treatment: carrying out high-temperature high-pressure treatment on the high-purity donkey-hide gelatin peptide solution to obtain a treatment solution; the conditions of the high-temperature high-pressure treatment comprise that the temperature is 110-130 ℃, the pressure is 0.1-0.3 MPa, and the time is 5-20 min;
(6) and (3) concentrating: carrying out reduced pressure heating concentration on the treatment liquid to obtain a concentrated liquid;
(7) drying to prepare powder: and carrying out spray drying on the concentrated solution to obtain the donkey-hide gelatin peptide powder.
Further, the preparation method of the ginseng oligopeptide comprises the following steps:
(1) pulverizing Ginseng radix, adding ethanol until the ethanol content is 60-70%, extracting, centrifuging, and filtering to obtain residue A and filtrate A;
(2) adding complex enzyme into the filter residue A in water for enzymolysis, and filtering to obtain filtrate B; the compound enzyme is flavourzyme, bromelain and consists of neutral protease, papain and trypsin;
(3) mixing the filtrate A and the filtrate B, passing through HPD-400 macroporous resin column, eluting with water, 10% ethanol, 20% ethanol and 30% ethanol respectively, collecting 10% ethanol, 20% ethanol and 30% ethanol eluate, and drying.
Further, the addition amount of the complex enzyme is 0.1-0.3% by mass of the ginseng, the pH is 5.5-8, the addition amount of the flavourzyme is 0.1-0.3% by mass of the ginseng, and the pH is 6-8; the addition amount of the bromelain is 0.05-0.2% by mass of the ginseng, and the pH value is 6-8; the mass ratio of the neutral protease to the papain to the trypsin is 1:2-4: 1-2.
Further, the preparation method of the walnut oligopeptide comprises the following steps:
(1) soaking in 1% NaOH solution at 65 deg.C for 10min, repeatedly washing with water, and removing walnut kernel peel;
(2) soaking peeled semen Juglandis at room temperature for 12 hr with a material-liquid ratio of 1: 12, grinding water at 60 deg.C, grinding with colloid mill, separating pulp from residue, filtering, and boiling for 5 min;
(3) adopting alkaline protease and flavourzyme with the ratio of 2: 1, dripping lactic acid or 1 mol/LNaHCO 3 solution in the reaction process to ensure that the pH value is constant at 7.5, heating to 95 ℃ after reacting for preset time, and preserving heat for 5min to stop enzymolysis;
(4) stirring the enzymolysis solution at 45 deg.C for 30min, debitterizing, filtering, collecting filtrate, concentrating the filtrate, and spray drying to obtain Juglandis oligopeptide powder.
The invention also aims to provide a preparation method of the composite donkey-hide gelatin peptide product for qi-tonifying and blood-nourishing comprehensive conditioning, which comprises the following steps:
(1) crushing and soaking raw materials: weighing red date, astragalus, medlar and bighead atractylodes rhizome according to the components, respectively crushing to 10-20 meshes, adding into 6-10 times of water, and soaking for 5-10 h;
(2) extraction: putting the red dates, the astragalus and the bighead atractylodes rhizome soaked in the step (1) into an extraction tank, heating to 80-100 ℃, extracting for 1-1.5h, and filtering to obtain a first extracting solution; adding 5-8 times of water into the residue, heating to 80-100 deg.C, extracting for 1-1.5 hr, and filtering to obtain second extractive solution;
(3) concentrating and fixing volume: mixing the two extracting solutions obtained in the step 2), and concentrating in a concentration tank until the mass fraction of the initial medicinal material is 7-23% to obtain a constant volume solution for later use;
(4) adding ingredients: sequentially adding resistant dextrin and L-glutamine according to the components, continuously stirring for 15-20min after completely adding, and keeping the temperature at 80-99 ℃ in the process to obtain concentrated solution;
(5) drying to prepare powder: and (4) carrying out spray drying on the concentrated solution to obtain composite component powder.
(6) Uniformly and slowly adding colla Corii Asini peptide powder, Ginseng radix oligopeptide and semen Juglandis oligopeptide into the composite powder according to the components, and stirring for 10-15min to obtain composite oligomeric colla Corii Asini peptide product.
Compared with the prior art, the invention has the following advantages and beneficial effects:
through a large number of experiments and researches, the traditional Chinese medicine composition has the effects of enriching blood and nourishing blood, is suitable for deficiency of both qi and blood, palpitation, amnesia, insomnia, dizziness and the like, improves the self hematopoietic function by improving the internal secretion and metabolism of a human body, has small side effect and definite curative effect, and avoids the problem that the existing western medicine treats symptoms and does not treat the root causes. And has good taste, no toxic or side effect, safety and easy popularization.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1:
the composite donkey-hide gelatin peptide product for benefiting qi and nourishing blood for comprehensive conditioning comprises the following raw and auxiliary materials in parts by weight: 50 parts of donkey-hide gelatin peptide, 25 parts of ginseng oligopeptide, 50 parts of walnut oligopeptide, 10 parts of red date, 5 parts of astragalus, 5 parts of medlar, 2 parts of bighead atractylodes rhizome, 18 parts of resistant dextrin and 10 parts of L-glutamine.
Further, the preparation method of the donkey-hide gelatin peptide comprises the following steps:
(1) dissolving: taking donkey-hide gelatin, carrying out superfine grinding, sieving with a 100-plus-500-mesh sieve, placing the donkey-hide gelatin in an enzymolysis reaction tank, adding 8-12 times of water, starting a stirring paddle, stirring for 0.5-1.0 h to enable the donkey-hide gelatin to be uniformly suspended, introducing steam into an interlayer of the tank, heating and boiling for 30min, closing the steam, and introducing cooling water to cool the temperature in the tank to 50-55 ℃;
(2) enzymolysis: adding a high-efficiency compound enzyme preparation with the dosage of 0.05-0.15% of the weight of the raw materials into the solution obtained in the step (1), uniformly mixing, and carrying out enzymolysis for 0.5-2 hours under natural conditions of no pH value adjustment and no temperature control to obtain a donkey-hide gelatin peptide solution; the high-efficiency compound enzyme preparation consists of 30-40 wt% of compound protease, 25-30 wt% of flavourzyme, 25-30 wt% of alkaline protease, 7-8 wt% of trypsin and 2-3 wt% of enzyme activator, wherein the enzyme activator contains a reducing agent and divalent metal ions; the reducing agent is dithiothreitol and/or beta-mercaptoethanol; the molar ratio of the reducing agent to the divalent metal ions in the enzyme activator is 1 (0.5-2); the divalent metal ions are a mixture of Mg2+, Zn2+ and Ca2 +;
(4) filtering with an inorganic nanofiltration ceramic membrane: filtering the donkey-hide gelatin peptide solution by adopting an inorganic nanofiltration ceramic membrane with the molecular weight cutoff of 800Da, wherein the obtained permeate is a high-purity donkey-hide gelatin peptide solution;
(5) high-temperature high-pressure treatment: carrying out high-temperature high-pressure treatment on the high-purity donkey-hide gelatin peptide solution to obtain a treatment solution; the conditions of the high-temperature high-pressure treatment comprise that the temperature is 110-130 ℃, the pressure is 0.1-0.3 MPa, and the time is 5-20 min;
(6) concentration: carrying out reduced pressure heating concentration on the treatment liquid to obtain a concentrated liquid;
(7) drying to prepare powder: and carrying out spray drying on the concentrated solution to obtain the donkey-hide gelatin peptide powder.
Further, the preparation method of the ginseng oligopeptide comprises the following steps:
(1) pulverizing Ginseng radix, adding ethanol until the ethanol content is 60-70%, extracting, centrifuging, and filtering to obtain residue A and filtrate A;
(2) adding complex enzyme into the filter residue A in water for enzymolysis, and filtering to obtain filtrate B; the compound enzyme is flavourzyme, bromelain and consists of neutral protease, papain and trypsin;
(3) mixing the filtrate A and the filtrate B, passing through HPD-400 macroporous resin column, eluting with water, 10% ethanol, 20% ethanol and 30% ethanol, respectively, collecting 10% ethanol, 20% ethanol and 30% ethanol eluate, and drying.
Further, the addition amount of the complex enzyme is 0.1-0.3% by mass of the ginseng, the pH is 5.5-8, the addition amount of the flavourzyme is 0.1-0.3% by mass of the ginseng, and the pH is 6-8; the addition amount of the bromelain is 0.05-0.2% by mass of the ginseng, and the pH value is 6-8; the mass ratio of the neutral protease to the papain to the trypsin is 1:2-4: 1-2.
Further, the preparation method of the walnut oligopeptide comprises the following steps:
(1) soaking in 1% NaOH solution at 65 deg.C for 10min, repeatedly washing with water, and removing walnut kernel peel;
(2) soaking peeled semen Juglandis at room temperature for 12 hr with a material-liquid ratio of 1: 12, grinding water at 60 deg.C, grinding with colloid mill, separating pulp from residue, filtering, and boiling for 5 min;
(3) adopting alkaline protease and flavourzyme with the ratio of 2: 1, dripping lactic acid or 1 mol/LNaHCO 3 solution in the reaction process to ensure that the pH value is constant at 7.5, heating to 95 ℃ after reacting for preset time, and preserving heat for 5min to stop enzymolysis;
(4) stirring the enzymolysis solution at 45 deg.C for 30min, debitterizing, filtering, collecting filtrate, concentrating the filtrate, and spray drying to obtain Juglandis oligopeptide powder.
A preparation method of a composite oligomeric donkey-hide gelatin peptide product for benefiting qi and nourishing blood for comprehensive conditioning comprises the following steps:
(1) crushing and soaking raw materials: weighing red date, astragalus, medlar and bighead atractylodes rhizome according to the components, respectively crushing to 10-20 meshes, adding into 6-10 times of water, and soaking for 5-10 h;
(2) extraction: putting the red dates, the astragalus and the bighead atractylodes rhizome soaked in the step (1) into an extraction tank, heating to 80-100 ℃, extracting for 1-1.5h, and filtering to obtain a first extracting solution; adding 5-8 times of water into the residue, heating to 80-100 deg.C, extracting for 1-1.5 hr, and filtering to obtain second extractive solution;
(3) concentrating and fixing volume: mixing the two extracting solutions in the step 2), and concentrating in a concentration tank until the mass fraction of the initial medicinal material is 7-23% to obtain a constant volume solution for later use;
(4) adding ingredients: sequentially adding resistant dextrin and L-glutamine according to the components, continuously stirring for 15-20min after completely adding, and keeping the temperature at 80-99 ℃ in the process to obtain concentrated solution;
(5) drying to prepare powder: and (4) carrying out spray drying on the concentrated solution to obtain composite component powder.
(6) Uniformly and slowly adding colla Corii Asini peptide powder, Ginseng radix oligopeptide and semen Juglandis oligopeptide into the composite powder according to the components, and stirring for 10-15min to obtain composite oligomeric colla Corii Asini peptide product.
Example 2:
the difference from the embodiment 1 is that the composite donkey-hide gelatin peptide product for qi-tonifying and blood-nourishing comprehensive conditioning comprises the following raw materials in parts by weight: 60 parts of donkey-hide gelatin peptide, 30 parts of ginseng oligopeptide, 30 parts of walnut oligopeptide, 8 parts of red date, 4 parts of astragalus, 4 parts of medlar, 1 part of bighead atractylodes rhizome, 8 parts of resistant dextrin and 3 parts of L-glutamine.
Example 3:
the difference from the embodiment 1 is that the composite donkey-hide gelatin peptide product for qi-tonifying and blood-nourishing comprehensive conditioning comprises the following raw materials in parts by weight: 70 parts of donkey-hide gelatin peptide, 25 parts of ginseng oligopeptide, 25 parts of walnut oligopeptide, 7 parts of red date, 3 parts of astragalus membranaceus, 3 parts of medlar, 1 part of bighead atractylodes rhizome, 8 parts of resistant dextrin and 1 part of L-glutamine.
Example 4:
the difference from the embodiment 1 is that the composite donkey-hide gelatin peptide product for qi-tonifying and blood-nourishing comprehensive conditioning comprises the following raw materials in parts by weight: 50 parts of donkey-hide gelatin peptide, 35 parts of ginseng oligopeptide, 35 parts of walnut oligopeptide, 9 parts of red date, 4 parts of astragalus, 4 parts of medlar, 1 part of bighead atractylodes rhizome, 14 parts of resistant dextrin and 5 parts of L-glutamine.
Test example 1:
in order to show the treatment effect of the invention, the invention carries out systematic clinical observation on 100 patients with deficiency of both qi and blood, and the specific results are as follows:
1. general data:
100 patients were outpatient cases with 500 males and 500 females, with the largest patient being 80 years of age and the smallest patient being 8 years of age. 100 patients were randomized into two groups of 50 patients, one group was the treatment group and the other group was the control group.
2. The treatment method comprises the following steps:
the patients in the treatment group all took the complex oligo-colla corii asini peptide preparation of example 13 times per day,
15g each time, and 30 days is a treatment course. The control group takes compound blood-tonifying granule 3 times a day, 20g each time, and 30 days as a treatment course.
3. Diagnostic criteria (traditional Chinese medicine):
deficiency of both qi and blood: laziness in speaking, weakness, spontaneous perspiration, pale complexion, palpitation, insomnia, dizziness, pale tongue with white coating, and thready and weak pulse.
4. The evaluation standard of curative effect is as follows:
(1) well: the disease is cured and the main symptoms disappear.
(2) And (3) effect is shown: grade 2 improvement of the chief complaints.
(3) And the effectiveness is as follows: grade 1 improvement of the chief complaints.
(4) And (3) invalidation: the main symptoms are not improved.
5. The treatment results are as follows:
table 1 shows the comparison of the treatment results of patients with deficiency of both qi and blood
Note: good effective rate, obvious effect and effective rate.
Test example 2:
first, experiment purpose
Experiments prove that the compound donkey-hide gelatin peptide product for tonifying qi and nourishing blood comprehensively regulates in example 2 has curative effects.
II, experimental samples: control group: white Phoenix Bolus of Black-bone chicken
Test groups: example 2 composite oligo-colla corii asini peptide granules for tonifying qi and nourishing blood and comprehensively conditioning
Third, Experimental methods
60 female Balb/c mice are taken, the weight is 20-22g, and the period is 6-8 weeks. The test group is divided into 6 groups, namely a normal group, a model group, a black-bone chicken white phoenix bolus control group and a compound low-polymer donkey-hide gelatin peptide particle high, medium and low dose test group. Except 10 normal control groups, the food is restricted by 75g/kg per day. Meanwhile, 0.3ml (about 10 drops) of blood is discharged every day for 7 consecutive days, the administration is started after the success of the model building is determined by taking blood for detection and hemogram on the seventh day, and the treatment administration and the continuous administration for 12 days are adopted in the study. The medicines are all prepared by distilled water, and the administration volume is 0.2ml/10 g; normal animals were gavaged with distilled water at a volume of 0.2ml/10g body weight. Blood was collected from the fundus venous plexus 40min after the last dose, and hemoglobin (Hb) and red blood cell content (RBC) and white blood cell count (WBC) in the blood were measured using a hematology analyzer.
Fourth, experimental results
TABLE 2 blood picture test of composite oligo-donkey-hide gelatin peptide particles
| Group of | WBC（*10 9 /L） | RBC(*10 12 /L) | HB(g/L) |
| Normal group | 7.37±0.15 ** | 8.78±0.24 ** | 126.12±2.33** |
| Model set | 4.11±0.15 | 5.84±0.26 | 109.94±2.61 |
| Wuji Baifeng Wan group | 5.64±0.18 | 7.85±0.33 * | 120.21±1.48 * |
| High dose test group | 6.37±0.29* | 7.72±0.45 * | 125.08±1.28 * |
| Middle dose test group | 5.91±0.24* | 7.38±0.34 * | 123.21±2.19 * |
| Low dose test group | 5.84±0.16 * | 7.21±0.27 * | 121.23±2.33 * |
Note: compared to the model group, p < 0.05, p < 0.01.
Fifth, experimental results
As can be seen from Table 2, after the test, compared with the normal group, the peripheral blood cells, the erythrocytes and the hemoglobin of the model group are all significantly reduced (p is less than 0.01); compared with the model group and the black-bone chicken white phoenix bolus, the high, medium and low dose test groups of the blood-enriching and nourishing health food can obviously improve the content of peripheral white blood cells, hemoglobin and red blood cells ((p is less than 0.5), which indicates that the blood-enriching and nourishing Chinese medicinal composition and the health food provided by the invention have ideal blood-enriching and nourishing functions.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. The composite donkey-hide gelatin peptide product for benefiting qi and nourishing blood comprehensive conditioning is characterized in that the raw and auxiliary materials are in the following weight ratio: 50-70 parts of donkey-hide gelatin peptide, 25-50 parts of ginseng oligopeptide, 25-50 parts of walnut oligopeptide, 7-10 parts of red date, 3-5 parts of astragalus membranaceus, 3-5 parts of medlar, 1-2 parts of bighead atractylodes rhizome, 8-18 parts of resistant dextrin and 1-10 parts of L-glutamine.
2. The composite donkey-hide gelatin peptide product for benefiting qi and nourishing blood comprehensive conditioning as claimed in claim 1, wherein the preparation method of the donkey-hide gelatin peptide comprises the following steps:
(1) dissolving: taking donkey-hide gelatin, carrying out superfine grinding, sieving with a 100-mesh and 500-mesh sieve, placing the donkey-hide gelatin in an enzymolysis reaction tank, adding 8-12 times of water, starting a stirring paddle, stirring for 0.5-1.0 h to enable the donkey-hide gelatin to be uniformly suspended, introducing steam into an interlayer of the tank body, heating and boiling for 30min, closing the steam, and introducing cooling water to cool the temperature in the tank to 50-55 ℃;
(2) enzymolysis: adding a high-efficiency compound enzyme preparation with the dosage of 0.05-0.15% of the weight of the raw materials into the solution in the step (1), uniformly mixing, and performing enzymolysis for 0.5-2 h under natural conditions without adjusting the pH value and controlling the temperature to obtain a donkey-hide gelatin peptide solution; the high-efficiency compound enzyme preparation consists of 30-40 wt% of compound protease, 25-30 wt% of flavourzyme, 25-30 wt% of alkaline protease, 7-8 wt% of trypsin and 2-3 wt% of enzyme activator, wherein the enzyme activator contains a reducing agent and divalent metal ions; the reducing agent is dithiothreitol and/or beta-mercaptoethanol; the molar ratio of the reducing agent to the divalent metal ions in the enzyme activator is 1 (0.5-2); the divalent metal ions are a mixture of Mg2+, Zn2+ and Ca2 +;
(4) filtering with an inorganic nanofiltration ceramic membrane: filtering the donkey-hide gelatin peptide solution by adopting an inorganic nanofiltration ceramic membrane with the molecular weight cutoff of 800Da to obtain a permeate which is a high-purity donkey-hide gelatin peptide solution;
(5) high-temperature high-pressure treatment: carrying out high-temperature high-pressure treatment on the high-purity donkey-hide gelatin peptide solution to obtain a treatment solution; the conditions of the high-temperature high-pressure treatment comprise that the temperature is 110-130 ℃, the pressure is 0.1-0.3 MPa, and the time is 5-20 min;
(6) concentration: carrying out reduced pressure heating concentration on the treatment liquid to obtain a concentrated liquid;
(7) drying to prepare powder: and carrying out spray drying on the concentrated solution to obtain the donkey-hide gelatin peptide powder.
3. The composite donkey-hide gelatin peptide product for benefiting qi and nourishing blood comprehensive conditioning as claimed in claim 1, wherein the preparation method of the ginseng oligopeptide comprises the following steps:
(1) pulverizing Ginseng radix, adding ethanol until the ethanol content is 60-70%, extracting, centrifuging, and filtering to obtain residue A and filtrate A;
(2) adding complex enzyme into the filter residue A in water for enzymolysis, and filtering to obtain filtrate B; the compound enzyme is flavourzyme, bromelain and consists of neutral protease, papain and trypsin;
(3) mixing the filtrate A and the filtrate B, passing through HPD-400 macroporous resin column, eluting with water, 10% ethanol, 20% ethanol and 30% ethanol respectively, collecting 10% ethanol, 20% ethanol and 30% ethanol eluate, and drying.
4. The composite donkey-hide gelatin peptide product for qi invigorating and blood nourishing comprehensive conditioning according to claim 3, wherein the additive amount of the composite enzyme is 0.1-0.3% by mass of the ginseng, the pH is 5.5-8, the additive amount of the flavourzyme is 0.1-0.3% by mass of the ginseng, and the pH is 6-8; the addition amount of the bromelain is 0.05-0.2% by mass of the ginseng, and the pH value is 6-8; the mass ratio of the neutral protease to the papain to the trypsin is 1:2-4: 1-2.
5. The composite donkey-hide gelatin peptide product for benefiting qi and nourishing blood comprehensive conditioning as claimed in claim 1, wherein the preparation method of the walnut oligopeptide comprises the following steps:
(1) soaking in 1% NaOH solution at 65 deg.C for 10min, repeatedly washing with water, and removing walnut kernel peel;
(2) soaking peeled semen Juglandis at room temperature for 12 hr with a material-liquid ratio of 1: 12, grinding water at 60 deg.C, grinding with colloid mill, separating pulp from residue, filtering, and boiling for 5 min;
(3) adopting alkaline protease and flavourzyme with the ratio of 2: 1, dripping lactic acid or 1 mol/LNaHCO 3 solution in the reaction process to ensure that the pH value is constant at 7.5, heating to 95 ℃ after reacting for preset time, and preserving heat for 5min to stop enzymolysis;
(4) stirring the enzymolysis solution at 45 deg.C for 30min, debitterizing, filtering, collecting filtrate, concentrating the filtrate, and spray drying to obtain Juglandis oligopeptide powder.
6. A preparation method of a composite oligomeric donkey-hide gelatin peptide product for benefiting qi and nourishing blood for comprehensive conditioning is characterized by comprising the following steps:
(1) crushing and soaking raw materials: weighing red date, astragalus, medlar and bighead atractylodes rhizome according to the components, respectively crushing to 10-20 meshes, adding into 6-10 times of water, and soaking for 5-10 h;
(2) extraction: putting the red dates, the astragalus and the bighead atractylodes rhizome soaked in the step (1) into an extraction tank, heating to 80-100 ℃, extracting for 1-1.5h, and filtering to obtain a first extracting solution; adding 5-8 times of water into the residue, heating to 80-100 deg.C, extracting for 1-1.5 hr, and filtering to obtain second extractive solution;
(3) concentrating and fixing volume: mixing the two extracting solutions in the step 2), and concentrating in a concentration tank until the mass fraction of the initial medicinal material is 7-23% to obtain a constant volume solution for later use;
(4) adding ingredients: sequentially adding resistant dextrin and L-glutamine according to the components, continuously stirring for 15-20min after completely adding, and keeping the temperature at 80-99 ℃ in the process to obtain concentrated solution;
(5) drying to prepare powder: and (4) carrying out spray drying on the concentrated solution to obtain composite component powder.
(6) Uniformly and slowly adding colla Corii Asini peptide powder, Ginseng radix oligopeptide and semen Juglandis oligopeptide into the composite powder according to the components, and stirring for 10-15min to obtain composite oligomeric colla Corii Asini peptide product.
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2022
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024074159A1 (en) * | 2023-04-11 | 2024-04-11 | 济宁学院 | Method for preparing small-molecule ass-hide glue polypeptide product |
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Application publication date: 20220923 |