CN111134091A - Artificial feeding method for diaphorina citri - Google Patents
Artificial feeding method for diaphorina citri Download PDFInfo
- Publication number
- CN111134091A CN111134091A CN202010096852.0A CN202010096852A CN111134091A CN 111134091 A CN111134091 A CN 111134091A CN 202010096852 A CN202010096852 A CN 202010096852A CN 111134091 A CN111134091 A CN 111134091A
- Authority
- CN
- China
- Prior art keywords
- diaphorina citri
- plastic cup
- host plant
- diaphorina
- citri
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000526125 Diaphorina citri Species 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 21
- 229920003023 plastic Polymers 0.000 claims abstract description 37
- 235000015097 nutrients Nutrition 0.000 claims abstract description 16
- 239000002689 soil Substances 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims description 20
- 241000158764 Murraya Species 0.000 claims description 17
- 230000004083 survival effect Effects 0.000 claims description 6
- 240000001899 Murraya exotica Species 0.000 claims description 5
- 235000008766 Murraya exotica Nutrition 0.000 claims description 5
- 235000009696 Murraya paniculata Nutrition 0.000 claims description 5
- 238000013138 pruning Methods 0.000 claims description 4
- 241000819999 Nymphes Species 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 claims description 2
- 238000012136 culture method Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 abstract description 6
- 239000000877 Sex Attractant Substances 0.000 abstract description 5
- 241000238631 Hexapoda Species 0.000 abstract description 4
- 238000012502 risk assessment Methods 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 3
- 238000012827 research and development Methods 0.000 abstract description 2
- 241000607479 Yersinia pestis Species 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 241000207199 Citrus Species 0.000 description 5
- 235000020971 citrus fruits Nutrition 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241001478315 Candidatus Liberibacter asiaticus Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241001672694 Citrus reticulata Species 0.000 description 1
- 240000000560 Citrus x paradisi Species 0.000 description 1
- 244000089795 Clausena lansium Species 0.000 description 1
- 235000008738 Clausena lansium Nutrition 0.000 description 1
- 241001468872 Fumagospora capnodioides Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- 241000725028 Kuwayama Species 0.000 description 1
- 240000002393 Murraya koenigii Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the field of agricultural science, and particularly relates to an artificial feeding method for diaphorina citri. Placing nutrient soil in the small transparent plastic cup, culturing diaphorina citri host plants, and arranging a drain hole at the bottom of the small plastic cup; and then, inoculating the diaphorina citri on the host plant, nesting and covering the host plant with transparent big plastic cups, wherein the tops of the covered big plastic cups are provided with air holes, and feeding the diaphorina citri under the conditions that the temperature is 28 +/-0.5 ℃, the relative humidity is 60 +/-5%, and the photoperiod is 14L:10D to obtain the diaphorina citri imagoes. The artificial feeding method of the diaphorina citri is simple and feasible, and is particularly suitable for indoor feeding and observation of the life history of the diaphorina citri. The method can provide insect source materials for the research and development of diaphorina citri sex pheromone, medicament screening, resistance monitoring and resistance risk assessment. Meanwhile, the feeding device is simple, the feeding cost is low, the fed host plants and the like can be repeatedly utilized, and the cost is saved.
Description
Technical Field
The invention belongs to the field of agricultural science, and particularly relates to an artificial feeding method for diaphorina citri.
Background
The Diaphorina citri Kuwayama belongs to the family hemiptera oblastomas, is a major pest of citrus, such as citrus, orange, pomelo, mandarin orange, lemon, wampee, murraya paniculata and the like, is also a main transmission medium for transmitting the most devastating diseases in citrus production, namely, the Asian species Candidatus Liberibacter asiaticus and the American species CaL. The diaphorina citri adults are scattered on leaves and tender shoots to suck, nymphs are clustered on new shoots, tender shoots and young leaves to be harmful, the young shoots and the young shoots are sucked with bud shoot juice, the damaged tender shoots and tender shoots are withered and withered, the new leaves are easy to fall off due to malformation, the growth of plants is seriously influenced, white secretion secreted by the nymphs is spread on branches and leaves to influence photosynthesis, and sooty mould can be caused. However, compared with the method of directly taking food as a pest, the biggest hazard of the diaphorina citri is the transmission of the yellow dragon disease germs and is an important way for transmitting the yellow dragon disease in the field. The adult diaphorina citri can fly and jump well, and the transfer of the adult diaphorina citri between diseased trees and healthy trees is a main cause for the spread of citrus greening disease in the field.
The effective control of the diaphorina citri is an important way for cutting off the propagation and spread of the citrus greening disease. At present, chemical control measures are mainly applied in China to control the harm of the diaphorina citri, the chemical control is usually the most effective measure in the peak period of the diaphorina citri, particularly in the young sprout period, but the safe control technology of the diaphorina citri is lacked in the mature period of fruits, particularly in the early stage of fruit picking. In the production, chemical prevention and control means are not suitable for the mature period of fruits, and biological prevention and control, physical prevention and control, pheromone attractant and other means can be selected for prevention and monitoring. However, there are few examples of successful biological control of diaphorina citri, and the rate of trapping diaphorina citri is low, in which case the development of diaphorina citri sex pheromone is particularly urgent. In agricultural production, pests are usually controlled by pesticides, the pesticide consumption is continuously increased, and the problem of pesticide resistance of agricultural pests is increasingly serious. With the continuous and deep research on drug resistance, resistance monitoring is very important in the early stage of pest resistance development, and indoor pest resistance risk assessment is a basic work for resistance control.
In the development process of diaphorina citri sex pheromone scientific research, a large number of mated and unmatched adults are needed, the unmatched adults are obtained by single-head feeding, and how to conveniently and quickly obtain the diaphorina citri with consistent insect age, high survival rate and relatively stable quantity plays a vital role in medicament screening, resistance monitoring and resistance risk assessment.
Disclosure of Invention
The invention aims to provide an artificial feeding method for diaphorina citri. The method can provide sufficient insect source materials for researching and developing diaphorina citri sex pheromone and monitoring the development level of the drug resistance of the diaphorina citri to common chemical agents.
The purpose of the invention is realized by the following technical scheme:
an artificial feeding method of diaphorina citri comprises the following steps:
placing nutrient soil in the small transparent plastic cup, culturing diaphorina citri host plants, and arranging a drain hole at the bottom of the small plastic cup; and then, inoculating the diaphorina citri on the host plant, nesting and covering the host plant with transparent big plastic cups, wherein the tops of the covered big plastic cups are provided with air holes, and feeding the diaphorina citri under the conditions that the temperature is 28 +/-0.5 ℃, the relative humidity is 60 +/-5%, and the photoperiod is 14L:10D to obtain the diaphorina citri imagoes.
Furthermore, the diameter of the bottom of the small plastic cup is 4.5cm, the diameter of the opening is 6.5cm, and the height is 8.5 cm; the diameter of the bottom of the big plastic cup is 5.5cm, the diameter of the opening is 8.5cm, and the height is 13.5 cm.
Furthermore, the nutrient soil is subjected to high-temperature deinsectization and sterilization treatment before use, the relative thickness of the nutrient soil is 2/5-4/5 of the height of a plastic cup, and the specific thickness is 4.5-6.5 cm.
Further, the host plant is murraya paniculata, and the culture method of the host plant comprises the following steps: transplanting the single seedling into a plastic cup, culturing by using nutrient soil, wherein the distance between the nutrient soil and the mouth of the plastic cup is 2-3 cm, pruning after successful survival and field planting of the host plant to ensure that the height of the survival and field planting of the host plant is 8-10 cm, maintaining the conditions of 28 +/-0.5 ℃ of temperature, 60 +/-5% of relative humidity and 14L:10D of photoperiod, culturing for 5-7 days, and germinating to grow young shoots for later use.
Further, the step of inoculating the diaphorina citri on the host plant comprises the following steps: when young shoots grow out of the murraya jasminorage, inoculating 4-5-year old nymphs of diaphorina citri to the young shoots of the murraya jasminorage, wherein the inoculation amount is 1 diaphorina citri nymph of each murraya jasminorage seedling, and feeding for 4-7 days to obtain the unmatched adult diaphorina citri. Can be used for scientific research experiments.
Further, the step of inoculating the diaphorina citri on the host plant comprises the following steps: and when young shoots grow out of the murraya jasminorage, 2 pairs of diaphorina citri are inoculated in a male-female 1:1 pairing mode, breeding for 15-20 days to obtain diaphorina citri adults, and after collecting the diaphorina citri, pruning and fertilizing the murraya jasminorage for repeated use.
The method of the invention has the following advantages and beneficial effects:
the artificial feeding method of the diaphorina citri is simple and feasible, and is particularly suitable for indoor feeding and observation of the life history of the diaphorina citri. The method can provide insect source materials for the research and development of diaphorina citri sex pheromone, medicament screening, resistance monitoring and resistance risk assessment. Meanwhile, the feeding device is simple, the feeding cost is low, the fed host plants and the like can be repeatedly utilized, and the cost is saved.
Drawings
FIG. 1 is a schematic view showing the structure of a small plastic cup of the rearing device in the embodiment of the present invention.
FIG. 2 is a schematic structural view of a large plastic cup of the rearing device in the embodiment of the present invention.
FIG. 3 is a schematic diagram of the structure of the host plant Murraya koenigii culture in the embodiment of the present invention.
Fig. 4 is a schematic structural diagram of single citrus psyllid feeding in the embodiment of the invention.
Fig. 5 is a schematic structural diagram of diaphorina citri population propagation in the embodiment of the invention.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
Examples
1) Manufacturing a feeding device:
taking a small transparent plastic cup, wherein the diameter of the bottom of the small plastic cup is 4.5cm, the diameter of an opening is 6.5cm, the height of the small plastic cup is 8.5cm, a drain hole is formed in the bottom of the small plastic cup, and the structural schematic diagram is shown in figure 1. The small plastic cup is filled with nutrient soil, the nutrient soil is subjected to high-temperature deinsectization and sterilization treatment before use, and the thickness of the nutrient soil in the small plastic cup is 6 cm. Murraya paniculata seedlings are purchased in the flower market, the seedlings are transplanted into a plastic cup, the plastic cup is cultured by using nutrient soil, after the survival and the planting are successful, the plastic cup is trimmed, the height of the seedlings exposed out of the nutrient soil is 8-10 cm, and the structural schematic diagram of the murraya paniculata culture is shown in fig. 3. Keeping the temperature at 28 +/-0.5 ℃, the relative humidity at 60 +/-5% and the photoperiod at 14L:10D, and germinating the murraya jasminorage seedlings to grow tender tips for 5-7 days for later use. Then, the diaphorina citri is inoculated, the transparent big plastic cup and the small plastic cup are nested and covered, the diameter of the bottom of the big plastic cup is 5.5cm, the diameter of the opening is 8.5cm, the height of the opening is 13.5cm, the top of the covered big plastic cup is provided with the air holes, and the structural schematic diagram is shown in figure 2.
2) Feeding single citrus psyllids:
when young shoots grow out of the murraya jasminorage seedlings, inoculating aged nymphs of 4-5 years old to the murraya jasminorage young shoots, keeping the temperature at 28 +/-0.5 ℃, the relative humidity at 60 +/-5% and the light period at 14L:10D under the condition that 1 citrus psylla nymph is inoculated to each murraya jasminorage seedling, and obtaining unmatched adult citrus psylla after 4-7 days for scientific research tests. The schematic structure of single-head diaphorina citri feeding is shown in figure 4.
3) And (3) carrying out diaphorina citri population propagation:
when young shoots grow out of the murraya jasminorage seedlings, 2 pairs of adult diaphorina citri are inoculated in a male-female 1:1 pairing mode, the temperature is kept at 28 +/-0.5 ℃, the relative humidity is kept at 60 +/-5%, the seedlings are propagated under the condition of a light cycle of 14L:10D, after 15-20 days, 50 heads of adult diaphorina citri are averagely propagated by each plant of murraya jasminorage, and after the diaphorina citri is collected, the murraya jasminorage seedlings are trimmed and fertilized and can be repeatedly used for multiple times. A schematic of the structure of the diaphorina citri population propagation is shown in figure 5.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (6)
1. An artificial feeding method of diaphorina citri is characterized by comprising the following steps:
placing nutrient soil in the small transparent plastic cup, culturing diaphorina citri host plants, and arranging a drain hole at the bottom of the small plastic cup; and then, inoculating the diaphorina citri on the host plant, nesting and covering the host plant with transparent big plastic cups, wherein the tops of the covered big plastic cups are provided with air holes, and feeding the diaphorina citri under the conditions that the temperature is 28 +/-0.5 ℃, the relative humidity is 60 +/-5%, and the photoperiod is 14L:10D to obtain the diaphorina citri imagoes.
2. The artificial feeding method of diaphorina citri according to claim 1, wherein: the diameter of the bottom of the small plastic cup is 4.5cm, the diameter of the opening is 6.5cm, and the height is 8.5 cm; the diameter of the bottom of the big plastic cup is 5.5cm, the diameter of the opening is 8.5cm, and the height is 13.5 cm.
3. The artificial feeding method of diaphorina citri according to claim 1, wherein: the nutrient soil is subjected to high-temperature deinsectization and sterilization treatment before use, the relative thickness of the nutrient soil is 2/5-4/5 of the height of a plastic cup, and the specific thickness is 4.5-6.5 cm.
4. The artificial feeding method of diaphorina citri according to claim 1, wherein: the host plant is murraya paniculata, and the culture method of the host plant comprises the following steps: transplanting the single seedling into a plastic cup, culturing by using nutrient soil, wherein the distance between the nutrient soil and the mouth of the plastic cup is 2-3 cm, pruning after successful survival and field planting of the host plant to ensure that the height of the survival and field planting of the host plant is 8-10 cm, maintaining the conditions of 28 +/-0.5 ℃ of temperature, 60 +/-5% of relative humidity and 14L:10D of photoperiod, culturing for 5-7 days, and germinating to grow young shoots for later use.
5. The artificial feeding method of diaphorina citri according to claim 4, wherein the step of inoculating diaphorina citri on the host plant comprises the following steps: when young shoots grow out of the murraya jasminorage, inoculating 4-5-year old nymphs of diaphorina citri to the young shoots of the murraya jasminorage, wherein the inoculation amount is 1 diaphorina citri nymph of each murraya jasminorage seedling, and feeding for 4-7 days to obtain the unmatched adult diaphorina citri.
6. The artificial feeding method of diaphorina citri according to claim 4, wherein: the method for inoculating the diaphorina citri on the host plant comprises the following steps: and when young shoots grow out of the murraya jasminorage, 2 pairs of diaphorina citri are inoculated in a male-female 1:1 pairing mode, breeding for 15-20 days to obtain diaphorina citri adults, and after collecting the diaphorina citri, pruning and fertilizing the murraya jasminorage for repeated use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010096852.0A CN111134091A (en) | 2020-02-17 | 2020-02-17 | Artificial feeding method for diaphorina citri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010096852.0A CN111134091A (en) | 2020-02-17 | 2020-02-17 | Artificial feeding method for diaphorina citri |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111134091A true CN111134091A (en) | 2020-05-12 |
Family
ID=70527537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010096852.0A Withdrawn CN111134091A (en) | 2020-02-17 | 2020-02-17 | Artificial feeding method for diaphorina citri |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111134091A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112430639A (en) * | 2020-11-20 | 2021-03-02 | 广西大学 | Method for determining pathogenicity of diaphorina citri endophytic fungi |
| CN113519466A (en) * | 2021-08-05 | 2021-10-22 | 广州威佰昆生物科技有限公司 | Method for feeding diaphorina citri by tissue culture murraya paniculata |
| CN113693028A (en) * | 2021-08-05 | 2021-11-26 | 广州威佰昆生物科技有限公司 | Method for treating diaphorina citri by microinjection |
| CN114600840A (en) * | 2022-02-25 | 2022-06-10 | 广西特色作物研究院 | Method for determining biological activity of diaphorina citri nymphs |
| CN114717335A (en) * | 2022-05-10 | 2022-07-08 | 赣南师范大学 | Method for obtaining CLas infected diaphorina citri polypide body |
| CN114794030A (en) * | 2022-06-16 | 2022-07-29 | 广东省农业科学院植物保护研究所 | Diaphorina citri drug resistance monitoring device and drug resistance determination method |
-
2020
- 2020-02-17 CN CN202010096852.0A patent/CN111134091A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112430639A (en) * | 2020-11-20 | 2021-03-02 | 广西大学 | Method for determining pathogenicity of diaphorina citri endophytic fungi |
| CN113519466A (en) * | 2021-08-05 | 2021-10-22 | 广州威佰昆生物科技有限公司 | Method for feeding diaphorina citri by tissue culture murraya paniculata |
| CN113693028A (en) * | 2021-08-05 | 2021-11-26 | 广州威佰昆生物科技有限公司 | Method for treating diaphorina citri by microinjection |
| CN114600840A (en) * | 2022-02-25 | 2022-06-10 | 广西特色作物研究院 | Method for determining biological activity of diaphorina citri nymphs |
| CN114717335A (en) * | 2022-05-10 | 2022-07-08 | 赣南师范大学 | Method for obtaining CLas infected diaphorina citri polypide body |
| CN114794030A (en) * | 2022-06-16 | 2022-07-29 | 广东省农业科学院植物保护研究所 | Diaphorina citri drug resistance monitoring device and drug resistance determination method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111134091A (en) | Artificial feeding method for diaphorina citri | |
| CN103385220B (en) | Scale breeding method for encarsia formosa | |
| CN106954592B (en) | Large-scale breeding method for Alliid lice spikemoss | |
| WO2015131751A1 (en) | Method of establishing cutting nursery for large-scale propagation of american sequoia seedling | |
| CN112586346B (en) | Tree eggplant, and cultivation method, rapid propagation method and application thereof | |
| CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
| CN106561575B (en) | Artificial feeding method for diaphorina citri | |
| CN107646684A (en) | A kind of breeding method of purpleback murdannia herb and its application | |
| CN105325296A (en) | Jackfruit tissue culture rapid propagation method | |
| CN102405751A (en) | Normal planting and yield increasing method for evodia rutaecarpa | |
| CN110521674A (en) | A kind of indoor feeding method of Ban Shi encyrtid | |
| CN112616780A (en) | Large-scale production method of belly-brightening glazed wasps | |
| CN104904531B (en) | A kind of take semen viciae fabae seedling and aphis craccivora as the Biological control of aphid method of savings carrier | |
| CN110301337A (en) | A kind of rubber tree cuttage and seedling culture method | |
| CN111492974A (en) | Tissue culture rapid propagation method of Eucalyptus robusta 1204 variety | |
| CN204579614U (en) | The device that moth egg lures collection knitted by a kind of coconut | |
| CN211832462U (en) | Artificial feeding device for diaphorina citri | |
| CN108112479B (en) | A kind of stem section of papaya sprout Bud Differentiation vacantly plants leaf promoting root growth method | |
| CN113558014B (en) | Large-scale production method of dormitory lice flea bees | |
| CN103430822B (en) | Aquaculture seed reproduction method for micro seed tubers of konjac | |
| CN105815214B (en) | A kind of blade seedling rapid propagation method of Ornithogalum caudatum | |
| CN101248758B (en) | Tissue culture method for fine stalk double butterflies | |
| CN110637665B (en) | Rapid cutting propagation method for citrus limonum | |
| CN110122332B (en) | Method for in-vitro culture and plant regeneration of broom corn millet young leaves | |
| CN113068614A (en) | Method for rapidly propagating high-quality seedlings of golden strelitzia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200512 |
|
| WW01 | Invention patent application withdrawn after publication |