CN111109005A - Cultivation method of wild ganoderma lucidum - Google Patents
Cultivation method of wild ganoderma lucidum Download PDFInfo
- Publication number
- CN111109005A CN111109005A CN202010067097.3A CN202010067097A CN111109005A CN 111109005 A CN111109005 A CN 111109005A CN 202010067097 A CN202010067097 A CN 202010067097A CN 111109005 A CN111109005 A CN 111109005A
- Authority
- CN
- China
- Prior art keywords
- ganoderma
- water
- culture medium
- ganoderma lucidum
- cultivating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to the technical field of vegetative propagation of wild lucid ganoderma, and particularly relates to a cultivation method of wild lucid ganoderma. A method for cultivating wild Ganoderma comprises obtaining Ganoderma entity, preparing mother seed, preparing stock seed, cultivating production seed, and cultivating Ganoderma fruiting body, wherein the raw materials of mother seed culture medium comprise rhizoma Solani Tuber osi, rice milk, sugar, agar, and water; the raw material of the stock culture medium comprises wood dust, platycodon grandiflorum powder, wheat bran, wheat, corn, gypsum, sugar, calcium carbonate and the balance of water; the raw materials of the production seed culture medium comprise sawdust, sweet potato powder, wheat, corn, soybean meal, sugar and the balance of water; the invention uses wild ganoderma as material to breed strains indoors, is not limited by seasons, can meet the quantity demand on production, can maintain the female parent character, effectively breeds high-quality ganoderma strains suitable for local development, realizes the large-scale production of ganoderma industry, and has better economic, social and ecological benefits.
Description
Technical Field
The invention belongs to the technical field of vegetative propagation of wild lucid ganoderma, and particularly relates to a cultivation method of wild lucid ganoderma.
Background
Ganoderma Lucidum is also known as Linzhongling and Qiongzhen (scientific name: Ganoderma Lucidum Karst) is a fruiting body of Ganoderma Lucidum of Polyporaceae, and is distributed in Longquan, Heilongjiang, Jilin, Hebei, Shandong, Anhuoshan, Jiangsu, Jiangxi, Hunan, Guizhou, Fujian, Guangdong, Guangxi and other provinces of China. Wherein the Ganoderma lucidum is planted in the areas of Longquan, Anhui and Shandong Taian in Zhejiang. Meanwhile, the ganoderma lucidum has a very wide application range. For the whole function of human body, the ganoderma lucidum has the function of bidirectionally regulating the function of human body, so that the balance of the function of human body is bidirectionally regulated on the whole, the immune system of human body is comprehensively and rapidly promoted, and the viscera and cells of human body are recovered to reach the standardization. In recent years, people are increasingly conscious of life quality and health care, and the development of food towards functions is promoted. The ganoderma lucidum has high natural medicinal value and edible value, has good effect on food, and continuously provides health care requirements for the life of people.
With the rapid development of social economy and the continuous improvement of the living standard of people, the demand of pure natural, safe and health-care food and medical supplies produced by taking ganoderma as a raw material is extremely large, and the great collection of ganoderma will cause the gradual depletion of wild ganoderma resources. Therefore, the domestication of the wild ganoderma lucidum strains has positive significance for developing the ganoderma lucidum industry, promoting artificial wild-like planting, providing powerful technical guarantee and realizing sustainable development of resources. The existing ganoderma lucidum wild strain domestication technology has the defects that the strain propagation survival rate is not high, the strain quality for production is poor, the existing ganoderma lucidum cultivation technology cannot meet the requirement of large-scale production, and the like.
Disclosure of Invention
The invention provides a cultivation method of wild ganoderma lucidum in order to overcome the defects of the prior art, provides a sterile material with sufficient quantity and high quality for the next step of propagation culture, and promotes the establishment of a rapid propagation system of the wild ganoderma lucidum in the production process.
The purpose of the invention is realized by the following technical scheme:
a method for cultivating wild Ganoderma comprises obtaining Ganoderma entity, preparing mother seed, preparing stock seed, cultivating production seed, and cultivating Ganoderma fruiting body,
the mother culture medium comprises the following raw materials in parts by weight: 100-300 parts of potatoes, 10-30 parts of rice milk, 10-30 parts of sugar, 10-30 parts of agar and 50-200 parts of water;
the stock culture medium comprises the following raw materials in percentage by weight: 10-70% of sawdust, 5-30% of platycodon grandiflorum powder, 5-30% of wheat bran, 10-50% of wheat, 5-20% of corn, 0.5-2% of gypsum, 0.5-2% of sugar, 1-3% of calcium carbonate and the balance of water;
the production seed culture medium comprises the following raw materials in percentage by weight: 30-60% of sawdust, 10-30% of sweet potato powder, 10-15% of wheat, 10-15% of corn, 1-5% of soybean meal, 1-3% of sugar and the balance of water;
the culture medium for cultivating the lucid ganoderma sporocarp comprises the following raw materials in parts by weight: 50-100 parts of sawdust, 20-30 parts of potato, 10-25 parts of soybean meal, 5-7 parts of avocado paste, 3-5 parts of calcium phosphate and 100-150 parts of water.
Preferably, the rice milk is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 2 to 5.
Preferably, the sweet potato powder is prepared by mixing and crushing stems, leaves and fruits of sweet potatoes.
Preferably, the avocado paste is prepared by pulping pitted avocados and water, wherein the weight ratio of the pitted avocados to the water is 1: 0.4 to 0.8.
Preferably, the mother seed culture medium, the stock seed culture medium, the production seed culture medium and the fruiting body culture medium are all sterilized by high pressure steam, and the four culture media are respectively maintained for 15-20 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃.
Preferably, the culture temperature of the mother seeds is 25-28 ℃, and the culture time is 13-18 days.
Preferably, the stock seeds are cultured for 38-42 days in an environment with the temperature of 25-28 ℃ and the illumination intensity of 50-600 LX.
Preferably, the production seeds are cultivated for 18-22 days in an environment with the daytime temperature of 18-22 ℃, the nighttime temperature of 12-16 ℃ and the illumination intensity of 100-800 LX.
Preferably, the lucid ganoderma sporocarp is cultured for 20-35 days in an environment with the temperature of 20-30 ℃ and the illumination intensity of 1000-2000 LX.
Compared with the prior art, the invention has the following advantages and positive effects:
1. the invention uses wild ganoderma lucidum as material to breed strains indoors, is not limited by seasons, can meet the quantity demand on production, and can maintain the female parent character; after the explant is subjected to stock seed preparation, high-quality production seeds can be continuously provided, the requirement of next step of enrichment culture can be met, and the large-scale production of the ganoderma lucidum is realized.
2. The time for collecting the wild lucid ganoderma is between 7 months and 9 months per year, the lucid ganoderma is in the mature period and grows vigorously, and the wild lucid ganoderma is used for domestication cultivation, so that the survival rate is high, and the proliferation rate is high.
3. The invention prepares culture medium according to the growth characteristics and different growth requirements of wild ganoderma, selects rice milk and sugar to prepare mother culture medium, because the rice milk is easy to degrade, the nutrient substances can be fully absorbed by the strain, the growth of ganoderma is promoted, and the rice milk and sugar are easy to obtain, the cost is low, the invention adds a certain amount of balloonflower powder in the original culture medium, because balloonflower not only can promote the growth of ganoderma strain, especially can promote the formation of ganoderma polysaccharide which is the effective component of ganoderma, the product quality is improved, the sweet potato powder which is prepared by cutting the stems, leaves and fruits of sweet potatoes is added in the production culture medium, because the sweet potato powder is rich in nutrient substances such as cellulose, potassium element and the like, the proliferation activity of the strain separated from tissue can be enhanced, the survival rate of the production seed is ensured, avocado contains β -phenylpropenoic acid, β -phenylpropenoic acid is a precursor compound for synthesizing anthocyanin, the content of ganoderma is an important factor for influencing the color and luster of ganoderma, the invention can effectively regulate the accumulation of ganoderma color and luster by controlling the culture medium of ganoderma sporophore and illumination conditions, and enable the cultured ganoderma to be good.
4. The method has the advantages of easily obtained materials, low cost, simple method, convenient operation and quality guarantee, can realize the establishment of a rapid propagation system of the ganoderma lucidum, and has better economic benefit, social benefit and ecological benefit.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the embodiments of the present invention are not limited to the scope of the examples. These examples are intended to illustrate the invention only and are not intended to limit the scope of the invention. In addition, various modifications may occur to those skilled in the art upon reading the present disclosure, and such equivalent variations are within the scope of the present invention as defined in the appended claims.
Example 1
(1) Obtaining a ganoderma lucidum entity: collecting wild Ganoderma with good growth, no disease and insect pest, and good color as explant at early 8 months of 2016, placing the collected wild Ganoderma in a sealed bag, immediately taking back to room, washing with tap water for 2-3 times, and wiping Ganoderma with absorbent cotton dipped with 1.5% detergent water solution;
(2) preparing a mother seed: 100g of potatoes, 10g of rice milk, 10g of sugar, 10g of agar and 50g of water are used as mother culture mediums, the rice milk is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 2, placing the mixture into a test tube, sterilizing the mixture for 15 to 20 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃ under high pressure to prepare a potato agar test tube for later use, inoculating the collected wild ganoderma lucidum fruiting bodies to the inclined plane of the potato agar test tube by a tissue separation method, culturing the wild ganoderma lucidum fruiting bodies at the constant temperature of 25 ℃ for 13 days to grow snow white mycelium, and selecting high-quality strains as mother seeds;
(3) preparing stock seeds: taking 10% of wood dust, 5% of platycodon grandiflorum powder, 5% of wheat bran, 10% of wheat, 5% of corn, 0.5% of gypsum, 0.5% of sugar, 1% of calcium carbonate and the balance of water as a stock culture medium, bagging, carrying out autoclaving for 15 minutes under the conditions of the pressure of 103.4kPa and the temperature of 121.3 ℃, inoculating a mother strain, and culturing for 38 days in an environment with the illumination intensity of 50LX at 25 ℃;
(4) cultivating production seeds: taking 30% of wood chips, 10% of sweet potato powder, 10% of wheat, 10% of corn, 1% of soybean powder, 1% of sugar and the balance of water as a production seed culture medium by weight percentage, carrying out autoclaving for 15 minutes at the pressure of 103.4kPa and the temperature of 121.3 ℃ for later use, mixing and crushing stems, leaves and fruits of sweet potatoes to obtain a ganoderma lucidum stock seed with strong growth vigor, inoculating the ganoderma lucidum stock seed to a test tube inclined plane filled with the production seed culture medium by using a tissue separation method, culturing for 18 days at the daytime temperature of 18 ℃, the nighttime temperature of 12 ℃ and the illumination intensity of 100LX to grow hypha, and selecting the required ganoderma lucidum as a production seed. And (3) completing the steps (1) to (4) on a super clean workbench, and investigating after 18d, wherein the hypha growth rate of the culture medium is 90.6%, and the hypha growth is normal.
(5) And (3) cultivating lucid ganoderma sporocarp: using 500 g of wood chips, 200g of potatoes, 100g of soybean meal, 50g of mashed avocado, 30g of calcium phosphate and 1000 g of water as a culture medium for cultivating lucid ganoderma sporocarp, wherein the mashed avocado is prepared by pulping pitted avocado and water, and the weight ratio of the pitted avocado to the water is 1: 0.4. the culture medium is prepared by autoclaving at 121.3 deg.C under 103.4kPa for 15min, inoculating production seeds, and culturing at 20 deg.C under 1000LX illumination intensity for 20 days.
Example 2
(1) Obtaining a ganoderma lucidum entity: collecting wild Ganoderma with good growth, no plant diseases and insect pests, and good color as explant in middle ten days of 8 months in 2016, placing the collected wild Ganoderma in a sealed bag, immediately taking back to room, washing with tap water for 2-3 times, and wiping Ganoderma with absorbent cotton dipped with 1.5% liquid detergent;
(2) preparing a mother seed: the method comprises the following steps of taking 200g of potatoes, 20g of rice milk, 20g of sugar, 20g of agar and 100g of water as mother culture media, wherein the rice milk is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 3, placing the mixture into a test tube, carrying out autoclaving for 18 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃ to prepare a potato agar test tube for later use, inoculating the collected wild ganoderma lucidum fruiting bodies to the inclined plane of the potato agar test tube by a tissue separation method, culturing at the constant temperature of 26 ℃ for 15 days to grow snow white mycelium, and selecting high-quality strains as mother strains;
(3) preparing stock seeds: taking 50% of wood dust, 10% of platycodon grandiflorum powder, 10% of wheat bran, 15% of wheat, 10% of corn, 1% of gypsum, 1% of sugar, 1% of calcium carbonate and the balance of water as a stock culture medium, bagging, carrying out autoclaving for 18 minutes under the conditions of the pressure of 103.4kPa and the temperature of 121.3 ℃, inoculating a mother strain, and culturing for 40 days in an environment with the temperature of 26 ℃ and the illumination intensity of 200 LX;
(4) cultivating production seeds: 55% of wood chips, 15% of sweet potato powder, 12% of wheat, 12% of corn, 3% of soybean powder, 1.5% of sugar and the balance of water are taken as a production seed culture medium by weight percentage, and are sterilized under high pressure for 18 minutes for later use under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃, the sweet potato powder is prepared by mixing and crushing stems, leaves and fruits of sweet potatoes, a ganoderma lucidum stock with strong growth vigor is selected, the ganoderma lucidum stock is inoculated to a test tube inclined plane filled with the production seed culture medium by a tissue separation method, hyphae can grow after the cultivation for 20 days in the environment with the daytime temperature of 20 ℃, the nighttime temperature of 14 ℃ and the illumination intensity of 300LX, and the required ganoderma lucidum is selected as a production seed. After 20 days, the rate of hyphae growth in the medium was 91.6%, and the hyphae growth was normal.
(5) And (3) cultivating lucid ganoderma sporocarp: taking 700 g of wood chips, 250 g of potatoes, 200g of soybean meal, 60 g of mashed avocado, 40 g of calcium phosphate and 1200 g of water as a culture medium for cultivating lucid ganoderma sporocarp, wherein the mashed avocado is prepared by pulping pitted avocado and water, and the weight ratio of the pitted avocado to the water is 1: 0.5. the culture medium is prepared by autoclaving at 121.3 deg.C under 103.4kPa for 18 min, inoculating production seed, and culturing at 25 deg.C under 1500LX illumination for 26 days.
Example 3
(1) Obtaining a ganoderma lucidum entity: collecting wild Ganoderma with good growth, no plant diseases and insect pests, and good color as explant in the 8 th late ten years of 2016, placing the collected wild Ganoderma in a sealed bag, immediately taking back to the room, washing with tap water for 2-3 times, and wiping the Ganoderma with absorbent cotton dipped with 1.5% liquid detergent;
(2) preparing a mother seed: 300g of potatoes, 30g of rice pulp, 30g of sugar, 30g of agar and 200g of water are used as mother culture mediums, the rice pulp is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 5, placing the mixture into a test tube, carrying out autoclaving for 20 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃ to prepare a potato agar test tube for later use, inoculating the collected wild ganoderma lucidum fruiting bodies to the inclined plane of the potato agar test tube by a tissue separation method, carrying out constant-temperature culture at 28 ℃ for 18 days to grow snow white mycelium, and selecting high-quality strains as mother strains;
(3) preparing stock seeds: taking 70% of sawdust, 30% of platycodon grandiflorum powder, 30% of wheat bran, 50% of wheat, 20% of corn, 2% of gypsum, 2% of sugar, 3% of calcium carbonate and the balance of water as a stock culture medium, bagging, carrying out autoclaving for 20 minutes under the conditions of the pressure of 103.4kPa and the temperature of 121.3 ℃, inoculating a mother strain, and culturing for 42 days in an environment with the light intensity of 600LX at 28 ℃;
(4) cultivating production seeds: 60% of wood chips, 30% of sweet potato powder, 15% of wheat, 15% of corn, 5% of soybean powder, 3% of sugar and the balance of water are taken as a production seed culture medium by weight percentage, the production seed culture medium is sterilized under high pressure for 20 minutes at the temperature of 121.3 ℃ under the pressure of 103.4kPa for later use, the sweet potato powder is prepared by mixing and crushing stems, leaves and fruits of sweet potatoes, a ganoderma stock seed with strong growth vigor is selected, the ganoderma stock seed is inoculated to a test tube inclined plane filled with the production seed culture medium by a tissue separation method, hyphae can grow after the test tube is cultured for 22 days in the environment with the daytime temperature of 22 ℃, the nighttime temperature of 16 ℃ and the illumination intensity of 800LX, and the required ganoderma is selected as a. After 22 days, the rate of hyphae growth in the medium was 89.6%, and the hyphae growth was normal.
(5) And (3) cultivating lucid ganoderma sporocarp: 1000 g of wood chips, 300g of potatoes, 250 g of soybean meal, 70g of mashed avocado, 50g of calcium phosphate and 1500 g of water are used as a culture medium for cultivating lucid ganoderma sporocarp, the mashed avocado is prepared by pulping pitted avocado and water, and the weight ratio of the pitted avocado to the water is 1: 0.6. the culture medium is prepared by autoclaving at 121.3 deg.C under 103.4kPa for 20 min, inoculating production seeds, and culturing at 30 deg.C under 2000LX illumination intensity for 35 days.
Example 4
(1) Obtaining a ganoderma lucidum entity: collecting wild Ganoderma with good growth, no disease and insect pest, and good color as explant in 2016 at the beginning of 9 months, placing the collected wild Ganoderma in a sealed bag, immediately taking back to room, washing with tap water for 2-3 times, and wiping Ganoderma with absorbent cotton dipped with 1.5% detergent water solution;
(2) preparing a mother seed: taking 150g of potatoes, 15g of rice milk, 12g of sugar, 18g of agar and 130g of water as mother culture media, wherein the rice milk is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 4, placing the mixture into a test tube, carrying out autoclaving for 16 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃ to prepare a potato agar test tube for later use, inoculating the collected wild ganoderma lucidum fruiting bodies to the inclined plane of the potato agar test tube by a tissue separation method, carrying out constant-temperature culture at 27 ℃ for 14 days to grow snow white mycelium, and selecting high-quality strains as mother strains;
(3) preparing stock seeds: taking 18% of sawdust, 15% of platycodon grandiflorum powder, 20% of wheat bran, 35% of wheat, 13% of corn, 1.2% of gypsum, 1.2% of sugar, 1.2% of calcium carbonate and the balance of water as a stock culture medium, bagging, carrying out autoclaving for 16 minutes under the conditions of the pressure of 103.4kPa and the temperature of 121.3 ℃, inoculating a mother strain, and culturing for 39 days in an environment with the light intensity of 400LX at the temperature of 27 ℃;
(4) cultivating production seeds: the method comprises the following steps of taking 35% of wood chips, 13% of sweet potato powder, 11% of wheat, 11% of corn, 2% of soybean powder, 1.5% of sugar and the balance of water as a production seed culture medium by weight, carrying out autoclaving for 16 minutes at the pressure of 103.4kPa and the temperature of 121.3 ℃ for later use, mixing and crushing stems, leaves and fruits of sweet potatoes to obtain the sweet potato powder, selecting a ganoderma lucidum stock with strong growth vigor, inoculating the sweet potato powder to a test tube inclined plane filled with the production seed culture medium by using a tissue separation method, culturing for 19 days at the daytime temperature of 19 ℃, the nighttime temperature of 13 ℃ and the illumination intensity of 200LX to grow hypha, and selecting the required ganoderma lucidum as a production seed. After 19 days, the rate of hyphae growth in the medium was 92.1%, and the hyphae growth was normal.
(5) And (3) cultivating lucid ganoderma sporocarp: taking 550 g of wood chips, 210 g of potatoes, 110 g of soybean meal, 55 g of mashed avocado, 32 g of calcium phosphate and 1100 g of water as a culture medium for cultivating lucid ganoderma sporocarp, wherein the mashed avocado is prepared by pulping pitted avocado and water, and the weight ratio of the pitted avocado to the water is 1: 0.7. the culture medium is prepared by autoclaving at 121.3 deg.C under 103.4kPa for 17 min, inoculating production seeds, and culturing at 22 deg.C under 1200LX illumination for 27 days.
Example 5
(1) Obtaining a ganoderma lucidum entity: collecting wild Ganoderma with good growth, no disease and insect pest, and good color as explant at early 8 months of 2016, placing the collected wild Ganoderma in a sealed bag, immediately taking back to room, washing with tap water for 2-3 times, and wiping Ganoderma with absorbent cotton dipped with 1.5% detergent water solution;
(2) preparing a mother seed: 230g of potatoes, 22g of rice milk, 22g of sugar, 22g of agar and 190g of water are used as mother culture mediums, the rice milk is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 2.5, placing the mixture into a test tube, carrying out autoclaving for 17 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃ to prepare a potato agar test tube for later use, inoculating the collected wild ganoderma lucidum fruiting bodies to the inclined plane of the potato agar test tube by using a tissue separation method, culturing at the constant temperature of 26 ℃ for 14 days to grow snow white mycelium, and selecting high-quality strains as mother seeds;
(3) preparing stock seeds: taking 65% of sawdust, 23% of platycodon grandiflorum powder, 23% of wheat bran, 40% of wheat, 16% of corn, 1.6% of gypsum, 1.8% of sugar, 2.5% of calcium carbonate and the balance of water as a stock culture medium, bagging, carrying out autoclaving for 17 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃, inoculating a mother strain, and culturing for 39 days in an environment with the light intensity of 450LX at 26 ℃;
(4) cultivating production seeds: 55% of wood chips, 24% of sweet potato powder, 12% of wheat, 12% of corn, 3.5% of soybean powder, 2.5% of sugar and the balance of water are taken as a production seed culture medium by weight percentage, and are sterilized under high pressure for 17 minutes for later use under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃, the sweet potato powder is prepared by mixing and crushing stems, leaves and fruits of sweet potatoes, a ganoderma lucidum stock seed with strong growth vigor is selected, the ganoderma lucidum stock seed is inoculated to a test tube inclined plane filled with the production seed culture medium by a tissue separation method, hyphae can be grown in 19 days by cultivating at 21 ℃ in the daytime, 13 ℃ at night and 700LX illumination intensity, and the required ganoderma lucidum is selected as a production seed. After 20 days, the rate of hyphae growth in the medium was 90.4%, and the hyphae growth was normal.
(5) And (3) cultivating lucid ganoderma sporocarp: 850 g of wood chips, 220 g of potatoes, 180 g of soybean meal, 56 g of mashed avocado, 45 g of calcium phosphate and 1300 g of water are used as a culture medium for cultivating lucid ganoderma sporocarp, the mashed avocado is prepared by pulping pitted avocado and water, and the weight ratio of the pitted avocado to the water is 1: 0.8. the culture medium is prepared by autoclaving at 121.3 deg.C under 103.4kPa for 17 min, inoculating production seeds, and culturing at 27 deg.C under 1300LX illumination intensity for 28 days.
Example 6
(1) Obtaining a ganoderma lucidum entity: collecting wild Ganoderma with good growth, no plant diseases and insect pests, and good color as explant in 7-month middle ten year of 2016, placing the collected wild Ganoderma in a sealed bag, immediately taking back to room, washing with tap water for 2-3 times, and wiping Ganoderma with absorbent cotton dipped with 1.5% liquid detergent;
(2) preparing a mother seed: taking 280g of potatoes, 28g of rice milk, 27g of sugar, 26g of agar and 170g of water as mother culture media, wherein the rice milk is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 3.5, placing the mixture into a test tube, carrying out autoclaving for 16 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃ to prepare a potato agar test tube for later use, inoculating the collected wild ganoderma lucidum fruiting bodies to the inclined plane of the potato agar test tube by a tissue separation method, culturing at the constant temperature of 27 ℃ for 16 days to grow snow white mycelium, and selecting high-quality strains as mother seeds;
(3) preparing stock seeds: taking 45% of sawdust, 25% of platycodon grandiflorum powder, 15% of wheat bran, 45% of wheat, 8% of corn, 1.5% of gypsum, 1.5% of sugar, 2.5% of calcium carbonate and the balance of water as a stock culture medium, bagging, carrying out autoclaving for 19 minutes under the conditions that the pressure is 103.4kPa and the temperature is 121.3 ℃, inoculating a mother strain, and culturing for 41 days in an environment with the light intensity of 350LX at 27 ℃;
(4) cultivating production seeds: 55% of wood chips, 25% of sweet potato powder, 14% of wheat, 14% of corn, 4% of soybean powder, 2% of sugar and the balance of water are taken as a production seed culture medium by weight percentage, the production seed culture medium is sterilized under high pressure for 18 minutes at the temperature of 121.3 ℃ under the pressure of 103.4kPa for later use, the sweet potato powder is prepared by mixing and crushing stems, leaves and fruits of sweet potatoes, a ganoderma stock seed with strong growth vigor is selected, the ganoderma stock seed is inoculated to a test tube inclined plane filled with the production seed culture medium by a tissue separation method, hyphae can grow after the test tube is cultured for 21 days in the environment with the daytime temperature of 21 ℃, the nighttime temperature of 15 ℃ and the illumination intensity of 750LX, and the required ganoderma is selected as a. After 21 days, the rate of hyphae growth in the medium was 89.9%, and the hyphae growth was normal.
(5) And (3) cultivating lucid ganoderma sporocarp: using 900 g of wood chips, 290 g of potatoes, 240 g of soybean meal, 65 g of mashed avocado, 45 g of calcium phosphate and 1400 g of water as a culture medium for cultivating lucid ganoderma sporocarp, wherein the mashed avocado is prepared by pulping pitted avocado and water, and the weight ratio of the pitted avocado to the water is 1: 0.6. the culture medium is prepared by autoclaving at 121.3 deg.C under 103.4kPa for 19 min, inoculating production seeds, and culturing at 28 deg.C under 1800LX illumination intensity for 30 days.
Comparative example 1
In the ganoderma lucidum cultivation method, the avocado component in the ganoderma lucidum fruiting body cultivation medium is removed on the basis of the cultivation method in the embodiment 1, so that the influence of the avocado component on the cultivated ganoderma lucidum is inspected, and other steps and parameters are kept unchanged.
Comparative example 2
In the ganoderma lucidum cultivation method, the avocado component in the ganoderma lucidum fruiting body cultivation medium is removed on the basis of the cultivation method in the embodiment 2, so that the influence of the avocado component on the cultivated ganoderma lucidum is inspected, and other steps and parameters are kept unchanged.
Comparative example 3
In the ganoderma lucidum cultivation method, the illumination condition for cultivating the ganoderma lucidum fruiting body is controlled to be 2100LX on the basis of the cultivation method in the embodiment 1, so that the influence of illumination on the cultivated ganoderma lucidum is inspected, and other steps and parameters are kept unchanged.
Comparative example 4
In the ganoderma lucidum cultivation method, the illumination condition of ganoderma lucidum fruiting body cultivation is controlled to 2200LX on the basis of the cultivation method in the embodiment 1, so that the influence of illumination on the cultivated ganoderma lucidum is inspected, and other steps and parameters are kept unchanged.
Comparative example 5
In the ganoderma lucidum cultivation method, the illumination condition for cultivating the ganoderma lucidum fruiting body is controlled to be 800LX on the basis of the cultivation method in the embodiment 1, so that the influence of illumination on the cultivated ganoderma lucidum is inspected, and other steps and parameters are kept unchanged.
Comparative example 6
In the ganoderma lucidum cultivation method, the illumination condition of ganoderma lucidum fruiting body cultivation is controlled to be 900LX on the basis of the cultivation method in the embodiment 1, so that the influence of illumination on the cultivated ganoderma lucidum is inspected, and other steps and parameters are kept unchanged.
Respectively taking 100g of ganoderma lucidum obtained by culturing in examples 1-6 and comparative examples 1-6, respectively adding 1L of ethanol with the volume concentration of 70% for anthocyanin extraction, extracting at 50 ℃ for 45min, centrifuging at 6000r/min for 15min, collecting supernatant, repeatedly extracting filter residue for 1 time, combining supernatants, and concentrating under reduced pressure to obtain crude anthocyanin extract; performing D101 macroporous resin adsorption on the crude anthocyanin extract at the flow rate of 2BV/h, then eluting by using 60 percent ethanol solution at the flow rate of 0.5BV/h, and collecting eluent; concentrating the eluate under reduced pressure, drying to obtain Ganoderma anthocyanin powder, weighing to obtain anthocyanin content, wherein the anthocyanin content in examples and comparative examples is shown in Table 1.
TABLE 1 examples and comparative anthocyanin content
| Anthocyanin content (mg) | |
| Example 1 | 156.4 |
| Example 2 | 162.2 |
| Example 3 | 164.8 |
| Example 4 | 153.7 |
| Example 5 | 158.9 |
| Example 6 | 156.7 |
| Comparative example 1 | 115.2 |
| Comparative example 2 | 114.6 |
| Comparative example 3 | 102.4 |
| Comparative example 4 | 107.1 |
| Comparative example 5 | 97.2 |
| Comparative example 6 | 108.5 |
Table 1 shows that the anthocyanin content obtained by the preparation method is higher, and the color and luster of the cultivated ganoderma lucidum are plump and the taste is good.
Claims (9)
1. A method for cultivating wild Ganoderma comprises obtaining Ganoderma fruiting body, preparing mother seed, preparing stock seed, cultivating production seed, and cultivating Ganoderma fruiting body,
the mother culture medium comprises the following raw materials in parts by weight: 100-300 parts of potatoes, 10-30 parts of rice milk, 10-30 parts of sugar, 10-30 parts of agar and 50-200 parts of water;
the stock culture medium comprises the following raw materials in percentage by weight: 10-70% of sawdust, 5-30% of platycodon grandiflorum powder, 5-30% of wheat bran, 10-50% of wheat, 5-20% of corn, 0.5-2% of gypsum, 0.5-2% of sugar, 1-3% of calcium carbonate and the balance of water;
the production seed culture medium comprises the following raw materials in percentage by weight: 30-60% of sawdust, 10-30% of sweet potato powder, 10-15% of wheat, 10-15% of corn, 1-5% of soybean meal, 1-3% of sugar and the balance of water;
the culture medium for cultivating the lucid ganoderma sporocarp comprises the following raw materials in parts by weight: 50-100 parts of sawdust, 20-30 parts of potato, 10-25 parts of soybean meal, 5-7 parts of avocado paste, 3-5 parts of calcium phosphate and 100-150 parts of water.
2. The cultivation method of wild ganoderma lucidum as claimed in claim 1, wherein the rice milk is a product obtained by pulping rice and water, and the weight ratio of the rice to the water is 1: 2 to 5.
3. The method for cultivating wild ganoderma lucidum as claimed in claim 1, wherein the sweet potato powder is prepared by mixing and crushing stems, leaves and fruits of sweet potatoes.
4. The cultivation method of wild ganoderma lucidum as claimed in claim 1, wherein the mashed avocado is prepared by pulping pitted avocado and water, wherein the weight ratio of the pitted avocado to the water is 1: 0.4 to 0.8.
5. The method for cultivating wild ganoderma lucidum as claimed in claim 1, wherein the mother culture medium, the stock culture medium, the production seed culture medium and the fruiting body culture medium are all sterilized by high pressure steam, and the four culture media are maintained at a pressure of 103.4kPa and a temperature of 121.3 ℃ for 15-20 minutes.
6. The method for cultivating wild ganoderma lucidum according to claim 1 or 2, wherein the mother seeds are cultivated at 25-28 ℃ for 13-18 days.
7. The method for cultivating wild Ganoderma lucidum according to any one of claims 1 to 5, wherein the stock seed is cultivated at 25 to 28 ℃ for 38 to 42 days in an environment with an illumination intensity of 50 to 600 LX.
8. The method for cultivating wild ganoderma lucidum according to claim 1 or 3, wherein the production seeds are cultivated for 18-22 days in an environment with a daytime temperature of 18-22 ℃, a nighttime temperature of 12-16 ℃ and an illumination intensity of 100-800 LX.
9. The method for cultivating wild Ganoderma lucidum according to any one of claims 1 to 5, wherein the fruiting body of Ganoderma lucidum is cultivated at 20-30 ℃ under illumination intensity of 1000-2000 LX for 20-35 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010067097.3A CN111109005B (en) | 2020-01-20 | 2020-01-20 | Cultivation method of wild ganoderma lucidum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010067097.3A CN111109005B (en) | 2020-01-20 | 2020-01-20 | Cultivation method of wild ganoderma lucidum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111109005A true CN111109005A (en) | 2020-05-08 |
| CN111109005B CN111109005B (en) | 2021-12-17 |
Family
ID=70491529
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010067097.3A Active CN111109005B (en) | 2020-01-20 | 2020-01-20 | Cultivation method of wild ganoderma lucidum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111109005B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186199A (en) * | 2014-07-31 | 2014-12-10 | 贵州科学院 | Method for artificial cultivation of ganoderma mastoporum pat fruit body |
| CN107226735A (en) * | 2017-05-12 | 2017-10-03 | 江苏绿洲园艺绿化有限公司 | A kind of oyster mushroom culture medium matter and preparation method thereof |
| CN107821008A (en) * | 2017-12-17 | 2018-03-23 | 安吉东来药用辅料有限责任公司 | A kind of special planting material of substituting stuff cultivation Se-rich lucid ganoderma and preparation method thereof |
| CN109429893A (en) * | 2018-10-18 | 2019-03-08 | 瀹垮饯 | A kind of breeding method of blood ganoderma lucidum |
-
2020
- 2020-01-20 CN CN202010067097.3A patent/CN111109005B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186199A (en) * | 2014-07-31 | 2014-12-10 | 贵州科学院 | Method for artificial cultivation of ganoderma mastoporum pat fruit body |
| CN107226735A (en) * | 2017-05-12 | 2017-10-03 | 江苏绿洲园艺绿化有限公司 | A kind of oyster mushroom culture medium matter and preparation method thereof |
| CN107821008A (en) * | 2017-12-17 | 2018-03-23 | 安吉东来药用辅料有限责任公司 | A kind of special planting material of substituting stuff cultivation Se-rich lucid ganoderma and preparation method thereof |
| CN109429893A (en) * | 2018-10-18 | 2019-03-08 | 瀹垮饯 | A kind of breeding method of blood ganoderma lucidum |
Non-Patent Citations (3)
| Title |
|---|
| 张甫涛等: "《种菇新技术荟萃》", 31 March 1991, 中国医药科技出版社 * |
| 燕红等: "《微生物资源及利用》", 30 April 2012, 哈尔滨工程大学出版社 * |
| 陈士林等: "《中药材无公害栽培生产技术规范》", 31 December 2018, 中国医药科技出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111109005B (en) | 2021-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102687640A (en) | Antrodia camphorata fungi liquid submerged culture method and antrodia camphorata fungi polysaccharide extraction method | |
| CN105993600A (en) | Method for ratification cultivation of pine bacteria | |
| CN106673851A (en) | Radix astragali seu hedysari edible mushroom culture medium and method for cultivating radix astragali seu hedysari edible mushrooms by utilizing culture medium | |
| CN107673853A (en) | Agrocybe cylindracea culture medium prepared by a kind of bagasse | |
| CN111534441A (en) | Rapid cultivation method of ganoderma lucidum, ganoderma lucidum oral liquid and preparation method thereof | |
| CN102726210B (en) | Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus | |
| CN105559068B (en) | It is a kind of to utilize composition for eating the acquisition of medicine fungi fermentation radix tetrastigme and preparation method thereof | |
| CN103030452B (en) | Method for preparing liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris | |
| CN111149618B (en) | Novel Wuzhi strain and artificial cultivation method | |
| CN103299822A (en) | Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method | |
| CN112703963A (en) | Method for cultivating oyster mushroom by using stevia rebaudiana leaf residues | |
| CN100397976C (en) | Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide | |
| CN110115196B (en) | Lucid ganoderma cultivation method | |
| CN111109005B (en) | Cultivation method of wild ganoderma lucidum | |
| CN106083407A (en) | A kind of agaric culture medium and fungus cultivation method thereof | |
| CN112931058B (en) | Separation and purification method of Moganshan wild Dictyophora rubrovalvata strain | |
| JPS60149321A (en) | Culture of mushrooms | |
| CN108440153A (en) | A kind of nutrient solution prescription and preparation method thereof of mist training romaine lettuce | |
| KR102147880B1 (en) | Manufacturing method of functional ginseng toenjang fused with zinc and selenium | |
| CN112514736A (en) | Method for cultivating lentinus edodes by using stevia rebaudiana leaf residues | |
| CN107048350B (en) | Nutritional whole powder prepared by implanting hericium erinaceus sporocarp into potato and production method thereof | |
| CN106718020B (en) | Auricularia polytricha cultivation method taking cassava stems as auxiliary materials | |
| CN113652295B (en) | Preparation method of flaxseed-morchella bi-directional fermentation mycoplasm and vegetable oil | |
| CN114711087B (en) | Method for cultivating edible fungi by using crop straw and application | |
| KR101582972B1 (en) | Raw Material Medium Composition for Culturing Mushroom and Method for Culturing Mushroom Using the Same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20200508 Assignee: Guangxi Zhiyao Technology Service Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION FORESTRY Research Institute Contract record no.: X2022450000460 Denomination of invention: A cultivation method of wild Ganoderma lucidum Granted publication date: 20211217 License type: Common License Record date: 20221228 |