CN110999752A - Treatment process of premenstrual ease tablet dregs - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention belongs to the technical field of plant cultivation, and discloses a treatment process of propranolol dregs, which comprises the following steps: step 1) blasting, step 2) preparing culture solution, step 3) performing biological fermentation, and step 4) preparing cutting material. The treatment process can fully utilize the medicine dregs, and is favorable for plant cultivation through a fermentation process.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a treatment process of propranolol decoction dregs.
Background
Chinese herbal medicines are the most important components in traditional medicine, and with the increase of market demand and the development of the traditional Chinese medicine industry, more and more traditional Chinese medicine residues are generated in the processing of traditional Chinese medicines. According to the report, Chinese medicine dregs produced by Chinese medicine enterprises in China are millions of tons every year, and how to reasonably treat the medicine dregs becomes a problem to be solved urgently. Only part of the dregs are used for cultivating feed, fertilizer, garden cultivation medium and edible fungi, most of the traditional Chinese medicine dregs are intensively stacked, buried or burned, the treatment cost is high, the discharged waste gas after treatment pollutes the air, the land is occupied, the social resource is seriously wasted, and the pollution problem of the traditional Chinese medicine dregs becomes a serious social problem. At present, a plurality of existing documents research on traditional Chinese medicine dregs, for example, a Chinese patent 'CN 103739325A' discloses a bio-organic fertilizer prepared from traditional Chinese medicine dregs, which can be applied to vegetable bases; chinese patent 'CN 108658687A' discloses a method for preparing an organic fertilizer by using traditional Chinese medicine dregs, which comprises spraying a compound bacterial liquid into the dregs, and then adding a certain amount of urea and monoammonium phosphate to prepare the organic fertilizer. The above method has the following disadvantages: 1) because different herb residues have different sources, substances which have antagonism to the bacterial strains can be remained, and the traditional Chinese medicine herb residues cannot be popularized to use; 2) a large amount of cellulose and vegetable protein exist in the medicine dregs, and the bacterial strains are difficult to completely degrade.
Jingqian' an tablet is the main variety of Xiangyu group. The existing product technology refers to the standard of the national food and drug administration to prepare premenstrual tension, and the formula is as follows:
radix bupleuri 400g fructus Aurantii 400g cortex Albizziae 320g radix Curcumae 320g
320g of nutgrass galingale rhizome, 240g of green tangerine peel, 240g of beautiful sweetgum fruit, 280g of tangerine seed and 240g of sweetgum fruit
320g of angelica, 320g of white peony root, 320g of szechuan lovage rhizome, 240g of poria cocos, 240g of rhizoma ligustici wallichii
Areca peel 240g and licorice root 120 g.
The preparation process comprises the steps of extracting volatile oil from the fourteen raw materials, namely seven raw materials of radix bupleuri, fructus aurantii, radix curcumae, rhizoma cyperi, pericarpium citri reticulatae viride, angelica sinensis, ligusticum wallichii and the like by a distillation method, preparing the volatile oil into an inclusion compound by β -cyclodextrin for standby, collecting the aqueous solution after distillation in another container, adding 8 times of water into medicine residues for decocting for 2 times, 1 hour for the first time and 0.5 hour for the second time, merging decoction, filtering, adding the rest seven raw materials of cortex albiziae and the like into water for decocting for 2 times, 10 times of water for decocting for 2 hours for the first time, 8 times of water for decocting for 1 hour for the second time, merging decoction, filtering, merging the filtrate with the aqueous solution after distillation, concentrating under reduced pressure to thick paste with the relative density of 1.20-1.25 (measured at 75 ℃), cooling, adding ethanol to enable the ethanol content to be 55%, standing for 24 hours, filtering, washing precipitates with 55% ethanol, filtering, merging the filtrate, recovering ethanol, concentrating under reduced pressure to the relative density to be 1.20-1.25 (measured) and obtaining clear paste, spray drying, adding β -cyclodextrin, preparing granules, drying.
The dregs obtained in the preparation process are premenstrual tension dregs. How to effectively treat the premenstrual ease tablet dregs is a technical problem faced by enterprises.
In order to inhibit the occurrence of plant diseases and insect pests, various special products for preventing and treating plant diseases and insect pests appear on the market, most of the products contain chemical components, and after the products are applied for many years, the physical structure of soil is changed, so that the nutrient loss of soil hardened by beds is serious, the fertility is reduced, the drug resistance of the plant diseases and insect pests in the soil is serious, the applied fertilizer cannot be effectively utilized, the production-input ratio is increased, the economic benefit of flower farmers is poor, and the enthusiasm of the flower farmers is greatly reduced. The chrysanthemum is a medicinal ornamental plant, cuttage is an important propagation mode, and the technical problem to be solved is how to apply the Qiandanpian traditional Chinese medicine dregs to chrysanthemum cultivation.
Disclosure of Invention
Aiming at the technical problems in the prior art, the invention provides a treatment process of premenstrual ease tablet medicine dregs.
The invention is realized by the following technical scheme:
the treatment process of the propranolol decoction dregs comprises the following steps: step 1) blasting, step 2) preparing culture solution, step 3) performing biological fermentation, and step 4) preparing cutting material.
Further, the treatment process comprises the following steps:
step 1) blasting treatment: drying the residues subjected to the first tabletting, then carrying out steam explosion pretreatment, and then carrying out explosion;
step 2) preparation of a culture solution: collecting the residues after blasting, mixing with peanut cake powder at a mass ratio of 2-3:1, stirring, adding 5-10 times of water, heating to 100 deg.C, boiling for 15-20min under heat preservation, and naturally cooling to room temperature to obtain culture solution;
step 3) biological fermentation: inoculating Trichoderma viride seed solution into the culture solution at an inoculation density of 1-5 × 108cfu/ml, culturing for 6-12h, inoculating Bacillus subtilis seed solution with inoculation density of 1-5 × 108continuously culturing cfu/ml for 60-72h to obtain composite fermentation liquor; in the whole culture process, the stirring speed is controlled to be 100rpm, the culture temperature is controlled to be 28-32 ℃, and the pH value in the culture process is controlled to be 7.0-7.2 by feeding ammonia water;
step 4), preparing a cutting material: mixing peat soil, river sand and vermiculite to obtain a mixture, then spraying composite fermentation liquor on the mixture, uniformly mixing, and standing for 1-2 days at 20-35 ℃ to obtain the cuttage material.
Preferably, the conditions of the steam explosion pretreatment are as follows: the pressure is 1.2-1.5MPa, and the retention time is 10-15 min.
Preferably, in the step 4), the mass ratio of the peat soil to the river sand to the vermiculite is 3-5:2-3: 3-3.
Preferably, in the step 4), the spraying amount is that the ratio of the composite fermentation broth: mixture =1L: spraying at a ratio of 10 kg.
Preferably, the preparation method of the trichoderma viride seed solution comprises the following steps: inoculating trichoderma viride to a PDA solid culture medium for culture to obtain a single colony; and selecting a single colony, inoculating the single colony into a PDA liquid culture medium, and performing seed culture to obtain a trichoderma viride seed solution.
Preferably, the preparation method of the bacillus subtilis seed solution comprises the following steps: inoculating bacillus subtilis to an LB solid culture medium for culture to obtain a single colony; and selecting a single colony, inoculating the single colony on an LB liquid culture medium, and performing seed culture to obtain a bacillus subtilis seed solution.
The invention comprises the following steps: the strain specifically used in the embodiment of the invention is Trichoderma viride ATCC 9275, and the Bacillus subtilis is ATCC 2233; other strains of the same species that function similarly can also be used, and the invention is not limited.
Compared with the prior art, the technical scheme of the invention brings a series of beneficial effects, and mainly comprises the following aspects:
the medicine residues of the first tablet contain a large amount of fibrin polymers, the bacterial strains are difficult to utilize, and the degradation is slow, the application firstly adopts steam explosion pretreatment to destroy the wrapping effect of cellulose substances on protein, so that the crystallization area of cellulose is destroyed, the porosity and the internal surface area of raw materials are increased, and the subsequent enzymatic hydrolysis of cellulose is facilitated; the residues and peanut cake powder after blasting can provide normal growth conditions for the strains, the raw materials are relatively cheap, and the fermentation cost can be reduced;
according to multiple tests, the phenomena of slow growth and partial death of the dregs of a decoction caused by the fact that aspergillus niger, yeast and the like cannot be effectively utilized through the dregs of a decoction before the inoculation are found, probably because partial substances in the dregs of a decoction generate antagonism to strains, the first inoculation of trichoderma viride can be normally propagated, enzymes such as cellulase and the like can be generated, the dregs of a decoction are used as a substrate to generate reducing sugar, the dregs of a decoction can be utilized by bacillus subtilis which is inoculated subsequently, the trichoderma viride and the bacillus subtilis have symbiotic relationship, the mutual promotion can be realized in the culture process, insecticidal toxins such as antibiotics and the like can be generated, pathogens in soil can be killed, multiple protection is performed on plants, and the survival;
the main determinant factor of the cuttage survival rate of the chamomile is cuttage materials, the peat soil is added to effectively fix the root system and promote the growth of the root system, but the conductivity is high, the rooting is not facilitated, and phytopathogens can be bred; according to the invention, the compound bacterial liquid generated by fermenting dregs is sprayed, so that the breeding of phytopathogen is inhibited, nutrients are provided, and part of undegraded fiber substances are contained, so that the water and moisture can be preserved, the air permeability is improved, and the conductivity of peat soil can be reduced; the compound fermentation liquor also contains organic acid, micromolecular protein and amino acid substances, can promote root absorption, is beneficial to rapid rooting of cuttings, and has thick roots, long roots and more roots.
Detailed Description
Those skilled in the art can modify the process parameters appropriately to achieve the desired results with reference to the disclosure herein. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the products and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations and modifications, or appropriate alterations and combinations, of the products and methods described herein may be made and utilized without departing from the spirit, scope, and spirit of the invention. For a further understanding of the present invention, reference will now be made in detail to the following examples.
Example 1
The treatment process of the propranolol decoction dregs comprises the following steps:
drying the residues subjected to the previous tabletting, then placing under the conditions of 1.2MPa of pressure and 15min of retention time for steam explosion pretreatment, and then carrying out explosion; collecting residues after blasting, mixing with peanut cake powder at a mass ratio of 3:1, stirring, adding 10 times of water, heating to 100 deg.C, boiling for 15min under heat preservation, naturally cooling to room temperature, inoculating Trichoderma viride seed solution with an inoculation density of 1 × 108cfu/ml, culturing for 12h, inoculating Bacillus subtilis seed solution with inoculation density of 1 × 108continuously culturing cfu/ml for 72 hours to obtain composite fermentation liquor; in the whole culture process, the stirring speed is controlled to be 100rpm, the culture temperature is 28 ℃, and the pH value in the culture process is controlled to be 7.0 by feeding ammonia water;
mixing peat soil, river sand and vermiculite according to a mass ratio of 3:2:3 to obtain a mixture, and then spraying composite fermentation liquor on the mixture, wherein the spraying amount is as follows: mixture =1L: spraying 10kg of the chrysanthemum cutting material, uniformly mixing, and standing for 2 days at 25 ℃ to obtain the chrysanthemum cutting material.
The preparation method of the trichoderma viride seed solution comprises the following steps: inoculating trichoderma viride to a PDA solid culture medium for culture to obtain a single colony; and selecting a single colony, inoculating the single colony into a PDA liquid culture medium, and performing seed culture to obtain a trichoderma viride seed solution.
The preparation method of the bacillus subtilis seed liquid comprises the following steps: inoculating bacillus subtilis to an LB solid culture medium for culture to obtain a single colony; and selecting a single colony, inoculating the single colony on an LB liquid culture medium, and performing seed culture to obtain a bacillus subtilis seed solution.
Example 2
The treatment process of the propranolol decoction dregs comprises the following steps:
drying the residues subjected to the previous tabletting, then placing under the conditions of 1.5MPa of pressure and 10min of retention time for steam explosion pretreatment, and then carrying out explosion; collecting residues after blasting, mixing with peanut cake powder at a mass ratio of 2:1, stirring, adding 8 times of water, heating to 100 deg.C, boiling for 20min under heat preservation, naturally cooling to room temperature, inoculating Trichoderma viride seed solution with an inoculation density of 2 × 108cfu/ml, culturing for 6h, inoculating Bacillus subtilis seed solution with inoculation density of 1 × 108continuously culturing cfu/ml for 60h to obtain a composite fermentation liquid; in the whole culture process, the stirring speed is controlled to be 100rpm, the culture temperature is controlled to be 30 ℃, and the pH value in the culture process is controlled to be 7.2 by feeding ammonia water;
mixing peat soil, river sand and vermiculite according to a mass ratio of 5:3:3 to obtain a mixture, and then spraying composite fermentation liquor on the mixture, wherein the spraying amount is as follows: mixture =1L: spraying 10kg of the above components, mixing uniformly, and standing for 2 days at 28 ℃ to obtain a cutting material.
Comparative example 1
The treatment process of the propranolol decoction dregs comprises the following steps:
drying the residues of the first-layer sliced medicinal herbs in the sun, mixing with peanut cake powder according to the mass ratio of 3:1, stirring uniformly, adding 10 times of water by weight, heating to 100 ℃, boiling for 15min under the condition of heat preservation, naturally cooling to room temperature, inoculating Trichoderma viride seed solution with the inoculation density of 1 × 108cfu/ml, culturing for 12h, then inoculating the seed solution of the bacillus subtilis, wherein the inoculation density is 1 multiplied by 108continuously culturing cfu/ml for 72 hours to obtain composite fermentation liquor; in the whole culture process, the stirring speed is controlled to be 100rpm, the culture temperature is 28 ℃, and the pH value in the culture process is controlled to be 7.0 by feeding ammonia water;
mixing peat soil, river sand and vermiculite according to a mass ratio of 3:2:3 to obtain a mixture, and then spraying composite fermentation liquor on the mixture, wherein the spraying amount is as follows: mixture =1L: spraying 10kg of the chrysanthemum cutting material, uniformly mixing, and standing for 2 days at 25 ℃ to obtain the chrysanthemum cutting material.
Comparative example 2
The treatment process of the propranolol decoction dregs comprises the following steps:
drying the residues subjected to the previous tabletting, then placing under the conditions of 1.2MPa of pressure and 15min of retention time for steam explosion pretreatment, and then carrying out explosion; collecting residues after blasting, mixing with peanut cake powder at a mass ratio of 3:1, stirring, adding 10 times of water, heating to 100 deg.C, boiling for 15min under heat preservation, naturally cooling to room temperature, inoculating Aspergillus niger seed solution with an inoculation density of 5 × 108cfu/ml, and culturing for 72h to obtain fermentation liquor; in the whole culture process, the stirring speed is controlled to be 100rpm, the culture temperature is 28 ℃, and the pH value in the culture process is controlled to be 7.0 by feeding ammonia water;
mixing peat soil, river sand and vermiculite according to a mass ratio of 3:2:3 to obtain a mixture, and then spraying fermentation liquor on the mixture, wherein the spraying amount is as follows: mixture =1L: spraying 10kg of the chrysanthemum cutting material, uniformly mixing, and standing for 2 days at 25 ℃ to obtain the chrysanthemum cutting material.
Comparative example 3
The treatment process of the propranolol decoction dregs comprises the following steps:
drying the residues subjected to the previous tabletting, then placing under the conditions of 1.2MPa of pressure and 15min of retention time for steam explosion pretreatment, and then carrying out explosion; collecting residues after blasting, mixing with peanut cake powder at a mass ratio of 3:1, stirring, adding 10 times of water, heating to 100 deg.CBoiling for 15min under the condition of heat preservation, naturally cooling to room temperature, inoculating the seed solution of trichoderma viride and the seed solution of bacillus subtilis, wherein the inoculation densities are 1 multiplied by 108cfu/ml, and culturing for 72h to obtain a composite fermentation broth; in the whole culture process, the stirring speed is controlled to be 100rpm, the culture temperature is 28 ℃, and the pH value in the culture process is controlled to be 7.0 by feeding ammonia water;
mixing peat soil, river sand and vermiculite according to a mass ratio of 3:2:3 to obtain a mixture, and then spraying composite fermentation liquor on the mixture, wherein the spraying amount is as follows: mixture =1L: spraying 10kg of the chrysanthemum cutting material, uniformly mixing, and standing for 2 days at 25 ℃ to obtain the chrysanthemum cutting material.
Comparative example 4
Mixing peat soil, river sand and vermiculite according to the mass ratio of 3:2:3 to obtain a mixture, namely the chrysanthemum cutting material.
Example 3
Cuttage test of September
And (3) experimental design: setting 6 treatment groups in total, 100 branches in each group, sterilizing, then soaking in ABT rooting powder solution (with the concentration of 2 g/L) for 30-60s, cutting, culturing for 20d, carefully digging out cutting seedlings in each group, and measuring the survival number, the length of roots and the number of roots. The 6 treatment groups were examples 1-2 and comparative examples 1-4, respectively. Except different cutting materials, the other operation modes are completely the same and have comparability. Specific results table 1:
TABLE 1
| Group of | Number of survivals | Average root length mm | Root number (strip) |
| Example 1 | 98 | 18.7 | 12.1 |
| Example 2 | 97 | 18.9 | 11.8 |
| Comparative example 1 | 92 | 18.1 | 11.4 |
| Comparative example 2 | 83 | 15.8 | 9.2 |
| Comparative example 3 | 89 | 17.3 | 10.6 |
| Comparative example 4 | 70 | 14.8 | 8.5 |
The number of rotted roots of the plants and the average fresh weight of the plants were also measured, as shown in table 2;
TABLE 2
| Group of | Number of rotten strips | Average fresh weight g of plant |
| Example 1 | 0 | 4.6 |
| Example 2 | 1 | 4.5 |
| Comparative example 1 | 4 | 4.3 |
| Comparative example 2 | 11 | 3.9 |
| Comparative example 3 | 6 | 4.0 |
| Comparative example 4 | 23 | 3.6 |
And (4) conclusion: as shown in Table 1-2, the indexes such as the number of survivors, the average root length, the number of roots, the number of rotters and the fresh weight of the plants are integrated, and the examples 1-2 are obviously superior to the comparative examples 1-4; compared with the conventional cutting material of the comparative example 4, the average root length is increased by 3.9mm, the number of roots is increased by 3.7, the absorption of roots to substances is improved, the average fresh weight is correspondingly increased by 1g, the survival number is increased by 28, and the root rot phenomenon does not occur. According to the invention, the trichoderma viride and the bacillus subtilis are inoculated in sequence, so that dregs of a decoction can be effectively utilized as a substrate to generate reducing sugar, the reducing sugar generated by early fermentation of the trichoderma viride can be utilized by the bacillus subtilis inoculated subsequently, the trichoderma viride and the bacillus subtilis have a symbiotic relationship, and can be mutually promoted in the culture process to generate insecticidal toxins such as antibiotics and the like, pathogens in soil can be killed, multiple protection is performed on plants, organic acid, small molecular protein and amino acid substances can be generated, an obvious supplementary and auxiliary effect is performed on conventional cuttage materials, root absorption can be promoted, quick rooting of cuttings is facilitated, and the cuttings are long in root, numerous in root and high in survival rate.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. The treatment process of the propranolol decoction dregs comprises the following steps: step 1) blasting, step 2) preparing culture solution, step 3) performing biological fermentation, and step 4) preparing cutting material.
2. The process according to claim 1, characterized in that it comprises the following steps:
step 1) blasting treatment: drying the residues subjected to the first tabletting, then carrying out steam explosion pretreatment, and then carrying out explosion;
step 2) preparation of a culture solution: collecting the residues after blasting, mixing with peanut cake powder at a mass ratio of 2-3:1, stirring, adding 5-10 times of water, heating to 100 deg.C, boiling for 15-20min under heat preservation, and naturally cooling to room temperature to obtain culture solution;
step 3) biological fermentation: inoculating a trichoderma viride seed solution into the culture solution, culturing for 6-12h, then inoculating a bacillus subtilis seed solution, and continuously culturing for 60-72h to obtain a composite fermentation broth; in the whole culture process, the stirring speed is controlled to be 100rpm, the culture temperature is controlled to be 28-32 ℃, and the pH value in the culture process is controlled to be 7.0-7.2 by feeding ammonia water;
step 4), preparing a cutting material: mixing peat soil, river sand and vermiculite to obtain a mixture, then spraying composite fermentation liquor on the mixture, uniformly mixing, and standing for 1-2 days at 20-35 ℃ to obtain the cuttage material.
3. The treatment process according to claim 2, wherein the conditions of the steam explosion pretreatment are as follows: the pressure is 1.2-1.5MPa, and the retention time is 10-15 min.
4. The treatment process according to claim 2, wherein in the step 4), the mass ratio of the peat soil to the river sand to the vermiculite is 3-5:2-3: 3-3.
5. The treatment process according to claim 2, wherein in the step 4), the spraying amount is that according to the ratio of the composite fermentation liquor: mixture =1L:10 kg.
6. The treatment process according to claim 2, wherein the preparation method of the trichoderma viride seed solution comprises the following steps: inoculating trichoderma viride to a PDA solid culture medium for culture to obtain a single colony; and selecting a single colony, inoculating the single colony into a PDA liquid culture medium, and performing seed culture to obtain a trichoderma viride seed solution.
7. The treatment process according to claim 2, wherein the preparation method of the bacillus subtilis seed solution comprises the following steps: inoculating bacillus subtilis to an LB solid culture medium for culture to obtain a single colony; and selecting a single colony, inoculating the single colony on an LB liquid culture medium, and performing seed culture to obtain a bacillus subtilis seed solution.
8. The process according to claim 2, wherein the inoculation densities of the Trichoderma viride seed solution and the Bacillus subtilis seed solution are both (1-5). times.108cfu/ml。
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| Publication number | Priority date | Publication date | Assignee | Title |
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