CN110946217A - Preparation method of microbial feed for ruminants - Google Patents
Preparation method of microbial feed for ruminants Download PDFInfo
- Publication number
- CN110946217A CN110946217A CN201911356797.8A CN201911356797A CN110946217A CN 110946217 A CN110946217 A CN 110946217A CN 201911356797 A CN201911356797 A CN 201911356797A CN 110946217 A CN110946217 A CN 110946217A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- fermentation liquor
- mixing
- preparing
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
- A23K50/15—Feeding-stuffs specially adapted for particular animals for ruminants containing substances which are metabolically converted to proteins, e.g. ammonium salts or urea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention provides a preparation method of a microbial feed for ruminants, which relates to the technical field of microbial feeds and comprises the following steps: (1) preparing a fermentation material I: uniformly spraying fermentation liquor I into the alfalfa/straw mixture; sealing, fermenting and drying to obtain a fermented material I; (2) preparing a fermentation material II: uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture, sealing, fermenting and drying to obtain a fermentation material II; (3) preparing a traditional Chinese medicine zymolyte: mixing the pulverized materials of radix astragali, herba Portulacae and folium Helianthi, adding cellulase and appropriate amount of water, and performing enzymolysis to obtain Chinese medicinal enzymatic hydrolysate; (4) uniformly mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to a proportion; the feed additive is scientific and reasonable in raw material matching, can provide comprehensive nutrition for ruminants such as cattle and sheep, is good in digestibility and high in utilization rate, can realize the effect of rapid fattening, is strong in ruminant immunity and low in diarrhea rate, and effectively improves the breeding benefit.
Description
Technical Field
The invention relates to the technical field of microbial feed, in particular to a preparation method of microbial feed for ruminants.
Background
In recent years, the restriction of feed resources gradually becomes the bottleneck of the development of the world feed industry and even livestock production. The concentrated feed resources (such as corn, bean pulp, fish meal and the like) are in short supply and have higher price, while the cheap coarse feed is abandoned or burnt in large quantities due to insufficient utilization by animals, thereby causing resource waste and environmental pollution. At present, the gap of the feed is inevitable, and the resources of high-quality protein feed are more tense. Therefore, attempting to replace the increasingly scarce conventional feed materials with new feed materials will be an inevitable trend in future feed development.
The microbial feed takes microbes and complex enzyme as biological feed starter strains, and raw materials of the microbial feed are converted into microbial mycoprotein, bioactive small peptide amino acid, microbial active probiotics and a complex enzyme preparation which are integrated into a biological fermentation feed. The product not only can make up amino acid which is easy to be lacked in conventional feed, but also can quickly convert the nutrient components of other raw feed materials, thereby achieving the effect of enhancing digestion, absorption and utilization.
The ruminant Chinese cattle and sheep breeding industry is the traditional livestock industry in China, in recent years, with the implementation of the plan of 'returning forest and returning field' in China, the breeding scale of cattle and sheep is mainly a captive breeding mode, the feed source is the guarantee of successful captive breeding of the ruminants, and the problem that the utilization rate and the conversion rate of coarse feed in the ruminant breeding industry of cattle, sheep and the like become to be solved urgently is solved. Therefore, the feed has important development significance for the development of animal husbandry in order to improve the utilization rate and the conversion rate of the coarse feed and improve the fattening effect of the feed on ruminants.
The Chinese patent with the application number of 201710205463.5 discloses a method for producing a composite microecological active biological feed for ruminants by using waste mushroom residues, which comprises the steps of mixing the waste mushroom residues generated after edible mushrooms are harvested with crushed crop straw powder, wetting and stirring with water, finally enabling the water content of the material to be 60-70%, inoculating 3-10% of a composite enzyme-producing microbial agent into the mixed material, fully stirring, inoculating and fermenting. The method takes the waste mushroom residues as the main raw material, is matched with crop straws, inoculates a plurality of enzyme-producing microorganisms, and produces the composite microbial ecological active biological feed for the ruminant through fermentation, has the advantages of simple and convenient preparation, environmental protection, no antibiotic in the product, wide raw material source and low cost, can improve the intestinal digestion environment of the ruminant, promote digestion, improve the growth of the ruminant, not only solves the secondary utilization problem of the mushroom residues, but also has important significance for the development of animal husbandry.
As a big agricultural country, China contains a large amount of waste straws to be treated, and the production of microbial feed by using the straws as main raw materials is practiced for many times. However, if the nutrition level of the straw microbial feed is to be improved, raw material compounding of the microbial feed is required, and a fermentation process is further optimized, so that the microbial feed with higher nutrition level is obtained.
Disclosure of Invention
The invention aims to provide a preparation method of a microbial feed for ruminants, wherein the raw materials are scientifically and reasonably matched, comprehensive nutrition can be provided for the ruminants such as cattle and sheep, the digestibility is good, the utilization rate is high, the effect of rapid fattening can be realized, the immunity of the ruminants is strong, the diarrhea rate is low, and the breeding benefit is effectively improved.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a preparation method of a microbial feed for ruminants comprises the following steps:
(1) preparing a fermentation material I: sequentially crushing the alfalfa hay and the dry straws, and then mixing the crushed materials of the alfalfa hay and the dry straws according to a certain proportion to obtain an alfalfa/straw mixture; uniformly spraying a fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 60-65%; uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 15-32 ℃, and drying after fermentation is finished to obtain a fermented material I;
the fermentation liquor I is prepared by mixing the following raw materials: salt, composite trace elements, a Brucella fermentation broth, a Bacillus licheniformis fermentation broth, glucose, urea, sodium dihydrogen phosphate, potassium chloride and water;
(2) preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a certain proportion to obtain a bran/rapeseed dregs mixture; uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 65-70%; uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 15-32 ℃, and drying after fermentation is finished to obtain a fermentation material II;
the fermentation liquor II is prepared by mixing the following raw materials: salt, composite trace elements, candida utilis fermentation liquor, saccharomyces cerevisiae fermentation liquor, bacillus subtilis fermentation liquor, glucose, urea and water.
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving astragalus membranaceus, purslane and sunflower leaves, mixing the crushed materials of the astragalus membranaceus, the purslane and the sunflower leaves to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, carrying out enzymolysis, collecting an enzymolysis liquid after the enzymolysis is finished, and carrying out reduced pressure concentration on the enzymolysis liquid to obtain a traditional Chinese medicine enzymolysis product;
(4) and uniformly mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to a certain proportion to prepare the microbial feed.
Preferably, in the step (1), the straw is rice straw or wheat straw.
Preferably, in the step (1), the viable bacteria concentration of the fermentation liquor of the saccharomyces boulardii is 1-4 × 109CFU/ml; the viable bacteria concentration of the bacillus licheniformis fermentation liquor is 2-5 multiplied by 108CFU/ml。
Preferably, in step (1), the fermentation broth I is prepared by mixing the following raw materials: 0.8-1.5% of salt, 0-0.3% of composite trace elements, 0.1-0.3% of a Bradycoccus fermentation broth, 0.1-0.3% of a Bacillus licheniformis fermentation broth, 1-1.5% of glucose, 0.5-1.1% of urea, 0.05-0.1% of sodium dihydrogen phosphate, 0.05-0.1% of potassium chloride and the balance of water.
Preferably, in the step (1), the mass ratio of the alfalfa hay to the dry straw crushed material is 1: 2 to 2.5.
Preferably, the step of(2) In the method, the viable bacteria concentration of the candida utilis fermentation liquor is 3-8 multiplied by 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 3-10 multiplied by 108CFU/ml; the viable bacteria concentration of the bacillus subtilis fermentation liquor is 3-6 multiplied by 109CFU/ml。
Preferably, in step (2), the fermentation broth I is prepared by mixing the following raw materials: 0.5-1.3% of salt, 0-0.3% of composite trace elements, 0.2-0.4% of candida utilis fermentation liquor, 0.1-0.2% of saccharomyces cerevisiae fermentation liquor, 0.1-0.2% of bacillus subtilis fermentation liquor, 0.5-1.3% of glucose, 1.2-1.8% of urea and the balance of water.
Preferably, in the step (2), the mass ratio of the bran to the rapeseed meal crushed material is 1: 0.3 to 0.7.
Preferably, in the step (3), the mass ratio of the astragalus membranaceus, the purslane and the sunflower leaf crushed material is 1: 0.5-2: 0.2 to 0.5; the water accounts for 7-9 times of the traditional Chinese medicine mixture, the cellulase accounts for 5-8% of the traditional Chinese medicine mixture, and enzymolysis is carried out at room temperature for 2.5-3 h.
Preferably, in the step (4), the mass ratio of the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte is 10: 2.5-4: 0.05 to 0.4.
The invention has the beneficial effects that:
1. after the straws and the alfalfa are fermented and fermented by using the Brucella yeast and the Bacillus licheniformis, the digestibility of the straws is remarkably improved, the cellulose content is effectively reduced, the palatability is enhanced, and the nutritional value is greatly improved. After the alfalfa is fermented, the crude protein growth rate is high, the nutritional ingredients are more comprehensive, and more comprehensive nutritional substances are provided for ruminants. The Bradymyces can adsorb pathogenic bacteria, degrade pathogenic bacteria toxin, balance flora, stimulate intestinal mucosa to synthesize powder immunoglobulin, improve local immunity, reduce diarrhea, and improve absorption and application of nutrients.
2. According to the invention, the rapeseed dregs and the bran are fermented jointly by adopting candida utilis, saccharomyces cerevisiae and bacillus subtilis, so that crude protein, water-soluble protein and the like have good increasing rates, and meanwhile, the content of small peptide is greatly improved, and the small peptide is easier to digest and absorb by ruminants. And the combination of candida utilis, saccharomyces cerevisiae and bacillus subtilis can effectively improve the digestibility of microbial feed and improve the metabolism.
3. The astragalus, the purslane and the sunflower leaves are subjected to enzymolysis, and the prepared traditional Chinese medicine zymolyte is easy to absorb and utilize by ruminants, can enhance the immunity of the ruminants, enhances the disease resistance and effectively reduces the diarrhea rate of the ruminants.
4. The feed additive is scientific and reasonable in raw material matching, can provide comprehensive nutrition for ruminants such as cattle and sheep, is good in digestibility and high in utilization rate, can realize the effect of rapid fattening, is strong in ruminant immunity and low in diarrhea rate, and effectively improves the breeding benefit.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a preparation method of a microbial feed for ruminants comprises the following steps:
(1) preparing a fermentation material I: the method comprises the following steps of sequentially crushing the alfalfa hay and the dry rice straws, and then crushing the alfalfa hay and the dry rice straws according to a proportion of 1: 2.5, mixing to obtain an alfalfa/straw mixture; uniformly spraying fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 62%; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 16-22 ℃, and drying after fermentation is finished to obtain a fermented material I.
The fermentation liquor I is prepared by mixing the following raw materials: 1.2% of salt and composite trace elements0.2% of element, 0.2% of saccharomyces boulardii fermentation liquor, 0.2% of bacillus licheniformis fermentation liquor, 1.2% of glucose, 0.8% of urea, 0.05% of sodium dihydrogen phosphate, 0.08% of potassium chloride and the balance of water; the viable bacteria concentration of the fermentation liquor of the saccharomyces boulardii is 2 multiplied by 109CFU/ml; the viable bacteria concentration of the Bacillus licheniformis fermentation liquor is 3 multiplied by 108CFU/ml。
(2) Preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a ratio of 1: mixing at a mass ratio of 0.5 to obtain a mixture of bran/rapeseed dregs; uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 70 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 16-22 ℃, and drying after fermentation is finished to obtain a fermentation material II.
The fermentation liquor II is prepared by mixing the following raw materials: 0.9% of salt, 0.2% of composite trace elements, 0.3% of candida utilis fermentation liquor, 0.15% of saccharomyces cerevisiae fermentation liquor, 0.15% of bacillus subtilis fermentation liquor, 1.2% of glucose, 1.5% of urea and the balance of water.
The viable bacteria concentration of the Candida utilis fermentation liquor is 5 multiplied by 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 8 multiplied by 108CFU/ml; the viable bacteria concentration of the bacillus subtilis fermentation liquor is 4 multiplied by 109CFU/ml。
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving the astragalus, the purslane and the sunflower leaves, and mixing the crushed materials of the astragalus, the purslane and the sunflower leaves according to a mass ratio of 1: 1: 0.3, mixing to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, wherein the mass of the water is 8 times that of the traditional Chinese medicine mixture, the mass of the cellulase is 7% of that of the traditional Chinese medicine mixture, and carrying out enzymolysis at room temperature for 2.5 hours; collecting enzymolysis liquid after enzymolysis, and concentrating the enzymolysis liquid under reduced pressure to obtain the Chinese medicinal zymolyte.
(4) Mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to the ratio of 10: 3.5: 0.2, and preparing the microbial feed.
Example 2:
a preparation method of a microbial feed for ruminants comprises the following steps:
(1) preparing a fermentation material I: the method comprises the following steps of sequentially crushing the alfalfa hay and the dry rice straws, and then crushing the alfalfa hay and the dry rice straws according to a proportion of 1: 2, mixing the components according to the mass ratio to obtain an alfalfa/straw mixture; uniformly spraying the fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 65 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at the temperature of 23-32 ℃, and drying after fermentation is finished to obtain a fermented material I.
The fermentation liquor I is prepared by mixing the following raw materials: 1% of salt, 0.2% of composite trace elements, 0.2% of Bradymyces fermentation liquor, 0.3% of Bacillus licheniformis fermentation, 1% of glucose, 0.8% of urea, 0.08% of sodium dihydrogen phosphate, 0.08% of potassium chloride and the balance of water; the viable bacteria concentration of the fermentation liquor of the saccharomyces boulardii is 3 multiplied by 109CFU/ml; the viable bacteria concentration of the Bacillus licheniformis fermentation liquor is 4 multiplied by 108CFU/ml。
(2) Preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a ratio of 1: mixing at a mass ratio of 0.5 to obtain a mixture of bran/rapeseed dregs; uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 70 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 23-32 ℃, and drying after fermentation is finished to obtain a fermentation material II.
The fermentation liquor II is prepared by mixing the following raw materials: 0.8% of salt, 0.2% of composite trace elements, 0.3% of candida utilis fermentation liquor, 0.1% of saccharomyces cerevisiae fermentation liquor, 0.1% of bacillus subtilis fermentation liquor, 1% of glucose, 1.5% of urea and the balance of water.
The viable bacteria concentration of the Candida utilis fermentation liquor is 5 multiplied by 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 8 multiplied by 108CFU/ml; the viable bacteria concentration of the bacillus subtilis fermentation liquor is 4 multiplied by 109CFU/ml。
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving the astragalus, the purslane and the sunflower leaves, and mixing the crushed materials of the astragalus, the purslane and the sunflower leaves according to a mass ratio of 1: 2: 0.5, mixing to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, wherein the mass of the water is 9 times that of the traditional Chinese medicine mixture, the mass of the cellulase is 8% of that of the traditional Chinese medicine mixture, and carrying out enzymolysis at room temperature for 3 hours; collecting enzymolysis liquid after enzymolysis, and concentrating the enzymolysis liquid under reduced pressure to obtain the Chinese medicinal zymolyte.
(4) Mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to the ratio of 10: 3.5: 0.2, and preparing the microbial feed.
Example 3:
a preparation method of a microbial feed for ruminants comprises the following steps:
(1) preparing a fermentation material I: sequentially crushing the alfalfa hay and the dry wheat straw, and then crushing the alfalfa hay and the dry wheat straw according to the proportion of 1: 2.5, mixing to obtain an alfalfa/straw mixture; uniformly spraying the fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 65 percent; uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at the temperature of 23-32 ℃, and drying after fermentation is finished to obtain a fermented material I;
the fermentation liquor I is prepared by mixing the following raw materials: 1.5% of salt, 0.1% of saccharomyces boulardii fermentation liquor, 0.2% of bacillus licheniformis fermentation liquor, 1.2% of glucose, 1% of urea, 0.08% of sodium dihydrogen phosphate, 0.08% of potassium chloride and the balance of water; the viable bacteria concentration of the fermentation liquor of the saccharomyces boulardii is 3 multiplied by 109CFU/ml; the viable bacteria concentration of the Bacillus licheniformis fermentation liquor is 5 multiplied by 108CFU/ml。
(2) Preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a ratio of 1: mixing at a mass ratio of 0.7 to obtain a mixture of bran/rapeseed dregs; uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 70 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 23-32 ℃, and drying after fermentation is finished to obtain a fermentation material II.
The fermentation liquor II is prepared by mixing the following raw materials: 1.3% of salt, 0.2% of composite trace elements, 0.3% of candida utilis fermentation liquor, 0.1% of saccharomyces cerevisiae fermentation liquor, 0.1% of bacillus subtilis fermentation liquor, 1.1% of glucose, 1.2% of urea and the balance of water.
The viable bacteria concentration of the Candida utilis fermentation liquor is 8 multiplied by 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 10 multiplied by 108CFU/ml; the viable bacteria concentration of the bacillus subtilis fermentation liquor is 3 multiplied by 109CFU/ml。
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving the astragalus, the purslane and the sunflower leaves, and mixing the crushed materials of the astragalus, the purslane and the sunflower leaves according to a mass ratio of 1: 2: 0.5, mixing to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, wherein the mass of the water is 7 times that of the traditional Chinese medicine mixture, the mass of the cellulase is 8% of that of the traditional Chinese medicine mixture, and carrying out enzymolysis at room temperature for 2.5 hours; collecting enzymolysis liquid after enzymolysis, and concentrating the enzymolysis liquid under reduced pressure to obtain the Chinese medicinal zymolyte.
(4) Mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to the ratio of 10: 2.5: 0.2, and preparing the microbial feed.
Example 4:
a preparation method of a microbial feed for ruminants comprises the following steps:
(1) preparing a fermentation material I: sequentially crushing the alfalfa hay and the dry wheat straw, and then crushing the alfalfa hay and the dry wheat straw according to the proportion of 1: 2, mixing the components according to the mass ratio to obtain an alfalfa/straw mixture; uniformly spraying fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 60 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 15-25 ℃, and drying after fermentation is finished to obtain a fermented material I.
The fermentation liquor I is prepared by mixing the following raw materials: 0.8% of salt, 0.3% of composite trace elements, 0.1% of saccharomyces boulardii fermentation broth, 0.1% of bacillus licheniformis fermentation broth, 1.5% of glucose, 1.1% of urea, 0.1% of sodium dihydrogen phosphate, 0.05% of potassium chloride and the balance of water; bradThe viable bacteria concentration of Detorula yeast fermentation liquor is 1 x 109CFU/ml; the viable bacteria concentration of the Bacillus licheniformis fermentation liquor is 3 multiplied by 108CFU/ml。
(2) Preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a ratio of 1: 0.3 to obtain a mixture of bran/rapeseed dregs; uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 65 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 15-25 ℃, and drying after fermentation is finished to obtain a fermentation material II.
The fermentation liquor II is prepared by mixing the following raw materials: 0.5% of salt, 0.4% of candida utilis fermentation liquor, 0.2% of saccharomyces cerevisiae fermentation liquor, 0.15% of bacillus subtilis fermentation liquor, 1.3% of glucose, 1.8% of urea and the balance of water.
The viable bacteria concentration of the Candida utilis fermentation liquor is 3 multiplied by 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 10 multiplied by 108CFU/ml; viable bacteria concentration of bacillus subtilis fermentation liquor is 6 multiplied by 109CFU/ml。
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving the astragalus, the purslane and the sunflower leaves, and mixing the crushed materials of the astragalus, the purslane and the sunflower leaves according to a mass ratio of 1: 0.5: 0.3, mixing to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, wherein the mass of the water is 9 times that of the traditional Chinese medicine mixture, the mass of the cellulase is 5% of that of the traditional Chinese medicine mixture, and carrying out enzymolysis at room temperature for 3 hours; collecting enzymolysis liquid after enzymolysis, and concentrating the enzymolysis liquid under reduced pressure to obtain the Chinese medicinal zymolyte.
(4) Mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to the ratio of 10: 3: 0.05, and preparing the microbial feed.
Example 5:
a preparation method of a microbial feed for ruminants comprises the following steps:
(1) preparing a fermentation material I: the method comprises the following steps of sequentially crushing the alfalfa hay and the dry rice straws, and then crushing the alfalfa hay and the dry rice straws according to a proportion of 1: 2.2, mixing to obtain an alfalfa/straw mixture; uniformly spraying fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 62%; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at the temperature of 20-28 ℃, and drying after fermentation is finished to obtain a fermented material I.
The fermentation liquor I is prepared by mixing the following raw materials: 1.2% of salt, 0.2% of composite trace elements, 0.3% of saccharomyces boulardii fermentation liquor, 0.3% of bacillus licheniformis fermentation liquor, 1% of glucose, 0.5% of urea, 0.05% of sodium dihydrogen phosphate, 0.1% of potassium chloride and the balance of water; the viable bacteria concentration of the fermentation liquor of the saccharomyces boulardii is 4 multiplied by 109CFU/ml; the viable bacteria concentration of the Bacillus licheniformis fermentation liquor is 2 multiplied by 108CFU/ml。
(2) Preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a ratio of 1: mixing at a mass ratio of 0.5 to obtain a mixture of bran/rapeseed dregs; uniformly spraying fermentation liquor II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 68 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 20-28 ℃, and drying after fermentation is finished to obtain a fermentation material II.
The fermentation liquor II is prepared by mixing the following raw materials: 0.8% of salt, 0.3% of composite trace elements, 0.4% of candida utilis fermentation liquor, 0.2% of saccharomyces cerevisiae fermentation liquor, 0.2% of bacillus subtilis fermentation liquor, 0.5% of glucose, 1.5% of urea and the balance of water.
The viable bacteria concentration of the Candida utilis fermentation liquor is 5 multiplied by 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 3 multiplied by 108CFU/ml; the viable bacteria concentration of the bacillus subtilis fermentation liquor is 5 multiplied by 109CFU/ml。
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving the astragalus, the purslane and the sunflower leaves, and mixing the crushed materials of the astragalus, the purslane and the sunflower leaves according to a mass ratio of 1: 1.5: 0.2, mixing to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, wherein the mass of the water is 8 times that of the traditional Chinese medicine mixture, the mass of the cellulase is 7% of that of the traditional Chinese medicine mixture, and carrying out enzymolysis at room temperature for 3 hours; collecting enzymolysis liquid after enzymolysis, and concentrating the enzymolysis liquid under reduced pressure to obtain the Chinese medicinal zymolyte.
(4) Mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to the ratio of 10: 4: 0.4, and preparing the microbial feed.
Example 6:
a preparation method of a microbial feed for ruminants comprises the following steps:
(1) preparing a fermentation material I: the method comprises the following steps of sequentially crushing the alfalfa hay and the dry rice straws, and then crushing the alfalfa hay and the dry rice straws according to a proportion of 1: 2.5, mixing to obtain an alfalfa/straw mixture; uniformly spraying fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 62%; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at the temperature of 20-28 ℃, and drying after fermentation is finished to obtain a fermented material I.
The fermentation liquor I is prepared by mixing the following raw materials: 1% of salt, 0.2% of composite trace elements, 0.2% of a Bradychis yeast fermentation broth, 0.3% of a bacillus licheniformis fermentation broth, 1.2% of glucose, 1% of urea, 0.08% of sodium dihydrogen phosphate, 0.08% of potassium chloride and the balance of water; the viable bacteria concentration of the fermentation liquor of the saccharomyces boulardii is 3 multiplied by 109CFU/ml; the viable bacteria concentration of the Bacillus licheniformis fermentation liquor is 4 multiplied by 108CFU/ml。
(2) Preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a ratio of 1: mixing at a mass ratio of 0.5 to obtain a mixture of bran/rapeseed dregs; uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 70 percent; and (3) uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 20-28 ℃, and drying after fermentation is finished to obtain a fermentation material II.
The fermentation liquor II is prepared by mixing the following raw materials: 0.8% of salt, 0.2% of composite trace elements, 0.3% of candida utilis fermentation liquor, 0.15% of saccharomyces cerevisiae fermentation liquor, 0.15% of bacillus subtilis fermentation liquor, 1% of glucose, 1.5% of urea and the balance of water.
The viable bacteria concentration of the Candida utilis fermentation liquor is 5 multiplied by 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 5 multiplied by 108CFU/ml; the viable bacteria concentration of the bacillus subtilis fermentation liquor is 5 multiplied by 109CFU/ml。
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving the astragalus, the purslane and the sunflower leaves, and mixing the crushed materials of the astragalus, the purslane and the sunflower leaves according to a mass ratio of 1: 1: 0.3, mixing to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, wherein the mass of the water is 8 times that of the traditional Chinese medicine mixture, the mass of the cellulase is 7% of that of the traditional Chinese medicine mixture, and carrying out enzymolysis at room temperature for 3 hours; collecting enzymolysis liquid after enzymolysis, and concentrating the enzymolysis liquid under reduced pressure to obtain the Chinese medicinal zymolyte.
(4) Mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to the ratio of 10: 3.5: 0.3, and preparing the microbial feed.
Cultivation test:
1. lamb breeding test
The method comprises the steps of selecting 600 healthy lambs with good growth vigor and uniform varieties, wherein the weight difference between individuals is not more than 1.2kg in the same sheep farm, randomly dividing the lambs into 5 groups, and dividing each group into 120 lambs, namely a test group 1-4 and a control group.
Preparation before the test: and (3) injecting the vaccine to the lambs to be tested, expelling the parasites, and entering the formal period after pre-testing for 10 days.
In the 60-day pilot period, the control group is fed with 0.2kg/10kg of traditional antibiotic additive feed, and the test groups 1-4 are fed with 0.2kg/10kg of microbial feed in the invention examples 1-4 and the same amount of forage. Feeding for 2 times at 9:00 and 17:00 times daily, feeding for 2 times at 10:00 and 18:00 times daily, and feeding traditional antibiotic additive feed/microorganism feed, water and fodder in sequence. After the end of the pilot period, the growth status of the lambs was calculated as shown in table 1.
Table 1:
as can be seen from Table 1, the microbial feed provided by the embodiment of the invention has a good breeding effect on lambs, is high in fattening speed, and enables the lambs to have strong resistance, low morbidity and low mortality.
2. Beef cattle breeding
100 healthy and well-growing beef cattle of which the weight difference of unified varieties and individual bodies is not more than 5kg are selected in the same mutton sheep farm and randomly divided into 5 groups, wherein each group comprises 20 beef cattle, and the groups are respectively a test group 1-4 and a control group.
Preparation before the test: injecting vaccine into beef cattle to be tested, expelling parasite, and entering formal period after pre-testing for 15 days.
The normal test period is 90 days, the control group is fed with 3kg/100kg of commercially available common concentrated feed every day, the test is 1-4 days, the control group is fed with 3kg/100kg of microbial feed every day, and the forage is fed freely. Feeding for 2 times at a ratio of 9:30 and 17:30 and feeding for 2 times at a ratio of 10:30 and 18:30 every day, and feeding commercial common concentrated feed/microbial feed, then feeding water and finally feeding forage. Cleaning the colony house every day, and regularly disinfecting.
After the end of the pilot period, the growth of the beef cattle was calculated as shown in table 2.
Table 2:
as can be seen from Table 2, the microbial feed provided by the embodiment of the invention has a good fattening effect on beef cattle, the highest fattening effect can be up to 126.3/kg per day, and compared with a control group, the fattening effect is improved by 16.6%, so that the economic benefit of beef cattle farmers can be obviously improved. Moreover, the incidence of diseases is low, even no incidence of diseases exists, and the microbial feed disclosed by the invention has a certain improvement on the immunity of beef cattle.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A preparation method of a microbial feed for ruminants is characterized by comprising the following steps:
(1) preparing a fermentation material I: sequentially crushing the alfalfa hay and the dry straws, and then mixing the crushed materials of the alfalfa hay and the dry straws according to a certain proportion to obtain an alfalfa/straw mixture; uniformly spraying a fermentation liquor I into the alfalfa/straw mixture to ensure that the water content in the alfalfa/straw mixture is 60-65%; uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 15-32 ℃, and drying after fermentation is finished to obtain a fermented material I;
the fermentation liquor I is prepared by mixing the following raw materials: salt, composite trace elements, a Brucella fermentation broth, a Bacillus licheniformis fermentation broth, glucose, urea, sodium dihydrogen phosphate, potassium chloride and water;
(2) preparing a fermentation material II: sequentially crushing bran and rapeseed dregs, and then mixing the crushed materials of the bran and the rapeseed dregs according to a certain proportion to obtain a bran/rapeseed dregs mixture; uniformly spraying a fermentation liquid II into the bran/rapeseed meal mixture to ensure that the water content in the bran/rapeseed meal mixture is 65-70%; uniformly mixing, placing in an ensiling bag for sealing, carrying out sealed fermentation at 15-32 ℃, and drying after fermentation is finished to obtain a fermentation material II;
the fermentation liquor II is prepared by mixing the following raw materials: salt, composite trace elements, candida utilis fermentation liquor, saccharomyces cerevisiae fermentation liquor, bacillus subtilis fermentation liquor, glucose, urea and water.
(3) Preparing a traditional Chinese medicine zymolyte: respectively crushing and sieving astragalus membranaceus, purslane and sunflower leaves, mixing the crushed materials of the astragalus membranaceus, the purslane and the sunflower leaves to obtain a traditional Chinese medicine mixture, adding cellulase and a proper amount of water into the traditional Chinese medicine mixture, carrying out enzymolysis, collecting an enzymolysis liquid after the enzymolysis is finished, and carrying out reduced pressure concentration on the enzymolysis liquid to obtain a traditional Chinese medicine enzymolysis product;
(4) and uniformly mixing the fermentation material I, the fermentation material II and the traditional Chinese medicine zymolyte according to a certain proportion to prepare the microbial feed.
2. The method of preparing a microbial feed for ruminants according to claim 1, wherein in the step (1), the straw is rice straw or wheat straw.
3. The method for preparing a microbial feed for ruminants according to claim 1, wherein in the step (1), the viable bacteria concentration of the fermentation broth of the saccharomyces boulardii is 1-4 x 109CFU/ml; the viable bacteria concentration of the bacillus licheniformis fermentation liquor is 2-5 multiplied by 108CFU/ml。
4. The method for preparing a microbial feed for ruminants according to claim 1, wherein in the step (1), the fermentation liquor I is prepared by mixing the following raw materials: 0.8-1.5% of salt, 0-0.3% of composite trace elements, 0.1-0.3% of a Bradycoccus fermentation broth, 0.1-0.3% of a Bacillus licheniformis fermentation broth, 1-1.5% of glucose, 0.5-1.1% of urea, 0.05-0.1% of sodium dihydrogen phosphate, 0.05-0.1% of potassium chloride and the balance of water.
5. The preparation method of the microbial feed for ruminants according to claim 1, wherein in the step (1), the mass ratio of the alfalfa hay to the dry straw crushed materials is 1: 2 to 2.5.
6. The method for preparing the microbial feed according to claim 1, wherein in the step (2), the viable bacteria concentration of the Candida utilis fermentation liquor is 3-8 x 108CFU/ml; the viable bacteria concentration of the saccharomyces cerevisiae fermentation liquor is 3 to10×108CFU/ml; the viable bacteria concentration of the bacillus subtilis fermentation liquor is 3-6 multiplied by 109CFU/m。
7. The method for preparing a microbial feed for ruminants according to claim 1, wherein in the step (2), the fermentation liquor I is prepared by mixing the following raw materials: 0.5-1.3% of salt, 0-0.3% of composite trace elements, 0.2-0.4% of candida utilis fermentation liquor, 0.1-0.2% of saccharomyces cerevisiae fermentation liquor, 0.1-0.2% of bacillus subtilis fermentation liquor, 0.5-1.3% of glucose, 1.2-1.8% of urea and the balance of water.
8. The method for preparing a microbial feed for ruminants according to claim 1, wherein in the step (2), the mass ratio of the bran to the rapeseed meal crushed material is 1: 0.3 to 0.7.
9. The method for preparing a microbial feed for ruminants according to claim 1, wherein in the step (3), the mass ratio of the astragalus membranaceus, the purslane and the crushed sunflower leaves is 1: 0.5-2: 0.2 to 0.5; the water accounts for 7-9 times of the traditional Chinese medicine mixture, the cellulase accounts for 5-8% of the traditional Chinese medicine mixture, and enzymolysis is carried out at room temperature for 2.5-3 h.
10. The method for preparing the microbial feed for the ruminants according to claim 1, wherein in the step (4), the mass ratio of the fermentation material I to the fermentation material II to the traditional Chinese medicine zymolyte is 10: 2.5-4: 0.05 to 0.4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911356797.8A CN110946217A (en) | 2019-12-25 | 2019-12-25 | Preparation method of microbial feed for ruminants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911356797.8A CN110946217A (en) | 2019-12-25 | 2019-12-25 | Preparation method of microbial feed for ruminants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110946217A true CN110946217A (en) | 2020-04-03 |
Family
ID=69984088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911356797.8A Withdrawn CN110946217A (en) | 2019-12-25 | 2019-12-25 | Preparation method of microbial feed for ruminants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110946217A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111903837A (en) * | 2020-07-15 | 2020-11-10 | 西安乐民反刍动物研究所 | Fermented nutritive salt for traditional Chinese medicine or traditional Chinese medicine residues and using method thereof |
-
2019
- 2019-12-25 CN CN201911356797.8A patent/CN110946217A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111903837A (en) * | 2020-07-15 | 2020-11-10 | 西安乐民反刍动物研究所 | Fermented nutritive salt for traditional Chinese medicine or traditional Chinese medicine residues and using method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106173363B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using edible fungus bran | |
| CN101390566B (en) | Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff | |
| CN110122658A (en) | A kind of fermentative feedstuff of microbe and its preparation method and application | |
| CN106173362A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Broussonetia papyrifera branch and leaf | |
| CN105076753A (en) | Method for preparing cattle and sheep feed by fermenting bagasse | |
| CN104041717A (en) | Special feed, prepared from distiller's grains, for laying hen during egg producing period and preparation method of feed | |
| CN101912040B (en) | Tomato residue biological fermentation feed and preparation method thereof | |
| CN101401615A (en) | Preparation method for preparing stalk protein feedstuff with microorganism stalk leaven | |
| CN111328923A (en) | Biological composite straw pellet feed and preparation method thereof | |
| CN105053535A (en) | Fermented crop straw composition and application thereof | |
| CN106173365A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Flos Jasmini Sambac slag | |
| CN114158648A (en) | Tomato peel residue biological fermentation feed, preparation method and application thereof | |
| CN118000288A (en) | Non-grain ecological feed and preparation method and feeding method thereof | |
| CN103156066A (en) | Feed taking desert cistanche drag as raw materials and preparation method thereof | |
| CN106173361A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with herbage | |
| CN106173364A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with corn straw | |
| CN107041457A (en) | A kind of biological feedstuff and preparation method thereof | |
| CN106212879A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Maninot esculenta crantz. stem and leaf | |
| CN106212916A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with sugarcane tail | |
| CN115067419A (en) | Application of feed additive and preparation method of earthworm fermentation liquor | |
| CN106212915A (en) | Ferment in second time produces method and the product of cattle and sheep complete feed | |
| CN106234755B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using bagasse | |
| CN108077616A (en) | A kind of biological straw fermented feed preparation method for fattening period live pig | |
| CN103229888B (en) | Method for preparing protein feedstuff through straw solid-state fermentation by using neurospora crassa | |
| CN104855725A (en) | Millet straw leavening technology for mixed granulated feed for ruminants, and mixed granulated feed for ruminants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200403 |
|
| WW01 | Invention patent application withdrawn after publication |