CN110915651A - Culture medium for curcuma zedoary tissue culture seedlings and preparation method thereof - Google Patents
Culture medium for curcuma zedoary tissue culture seedlings and preparation method thereof Download PDFInfo
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- CN110915651A CN110915651A CN201911114139.8A CN201911114139A CN110915651A CN 110915651 A CN110915651 A CN 110915651A CN 201911114139 A CN201911114139 A CN 201911114139A CN 110915651 A CN110915651 A CN 110915651A
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- China
- Prior art keywords
- tissue culture
- benzylaminopurine
- culture medium
- indolebutyric acid
- agar
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- 240000009138 Curcuma zedoaria Species 0.000 title claims abstract description 26
- 239000001963 growth medium Substances 0.000 title claims abstract description 16
- 235000003405 Curcuma zedoaria Nutrition 0.000 title claims description 24
- 239000001812 curcuma zedoaria berg. rosc. Substances 0.000 title claims description 15
- 235000019509 white turmeric Nutrition 0.000 title claims description 15
- 235000014375 Curcuma Nutrition 0.000 title claims description 11
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 title claims description 11
- 238000002360 preparation method Methods 0.000 title claims description 7
- 244000164480 Curcuma aromatica Species 0.000 title claims 2
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 14
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229920001817 Agar Polymers 0.000 claims abstract description 12
- 239000008272 agar Substances 0.000 claims abstract description 12
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 8
- 229930006000 Sucrose Natural products 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- 239000008101 lactose Substances 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 8
- 239000005720 sucrose Substances 0.000 claims abstract description 8
- 239000003833 bile salt Substances 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000004806 packaging method and process Methods 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- 150000003839 salts Chemical class 0.000 claims abstract description 4
- 239000011780 sodium chloride Substances 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 5
- 239000002689 soil Substances 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 12
- 244000163122 Curcuma domestica Species 0.000 description 11
- 238000002791 soaking Methods 0.000 description 4
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 235000003392 Curcuma domestica Nutrition 0.000 description 2
- 241000963390 Curcuma wenyujin Species 0.000 description 2
- 235000003394 Curcuma wenyujin Nutrition 0.000 description 2
- 241000234299 Zingiberaceae Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 235000003373 curcuma longa Nutrition 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 235000013976 turmeric Nutrition 0.000 description 2
- 241000213006 Angelica dahurica Species 0.000 description 1
- 235000004359 Artemisia pontica Nutrition 0.000 description 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
- 240000006891 Artemisia vulgaris Species 0.000 description 1
- 244000205754 Colocasia esculenta Species 0.000 description 1
- 235000006481 Colocasia esculenta Nutrition 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000028446 budding cell bud growth Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium for a curcuma zedoary tissue culture seedling, which comprises the following components in parts by mass: 8g of lactose; 5g of ox gall salt; 0.2mg of 6-benzylaminopurine; 0.4mg indolebutyric acid; 30g of sucrose; 6g of agar powder; 5g of sodium chloride; 1000ml of water; the manufacturing method comprises the following steps: mixing the above components except lactose, ox bile salt, 6-benzylaminopurine, indolebutyric acid, sucrose and agar, heating to dissolve, adjusting pH to 5.8 + -0.2 after sterilization, adding agar, 6-benzylaminopurine and indolebutyric acid, heating to melt, adding the rest components, shaking, packaging, sterilizing with high pressure steam at 121 deg.C for 15 min, cooling to 60 deg.C, and pouring into flat dish. The curcuma zedoary tissue culture seedling is subjected to tissue culture by adopting the culture medium, the survival rate is over 90 percent, the period is short, the pollution is little, the production cost is low, and the large-scale planting is facilitated.
Description
Technical Field
The invention relates to the technical field of planting, in particular to a culture medium for a curcuma zedoary tissue culture seedling and a preparation method thereof.
Background
Zedoary, its name \33921, medicine, dicyclopentadieny, green ginger, black heart ginger, turmeric, latin literature name: curcuma zedoaria (Christm.) Rosc, Curcuma zedoaria is a perennial root herb of Curcuma of Zingiberaceae, dried rhizome of Curcuma zedoaria Curcumaeocaeruulis Val, Curcuma zedoaria Kwangsiensis S.G.Lee et C.F. Liang or Curcuma wenyuma wenyujin Y.H. Chen et C.Ling of Zingiberaceae. The latter is commonly called "Wen zedoary". Collecting withered stems and leaves in winter, cleaning, steaming or decocting until they are thoroughly soaked, sun drying or low temperature drying, and removing fibrous root and impurities. The plant height is about 1 m; the rhizome is cylindrical and fleshy, has camphor-like fragrance and is light yellow or white; the root is slender or the end is expanded into a tuberous root. Cultivated or wild in the shade of forest, distributed in India, Malaysia and around China. Can be used for treating qi circulation promoting, analgesic, stagnation removing, blood stasis dispelling, and blood stasis dispelling effects.
The curcuma zedoary has strict requirements on the ecological environment and is suitable for narrow planting areas. The soil is preferably soil with deep soil layer, good drainage, loose and fertile upper layer and compact lower layer, and is not suitable for planting in waterlogged depression. The Curcuma wenyujin (Curcuma wenyujin) should be selected from the soil with sufficient sunlight, moist soil, fertile and loose alluvial soil and sandy soil, and the clay should not be planted. The soil requires loose upper layer and compact lower layer, and avoids the root tuber from entering the soil too deeply and inconvenient excavation. The vegetable garden is fertile and loose, the land is suitable for planting, and the wheat field can be planted.
After the land is selected, 3000-4000 kg of decomposed manure or compost and 40-50 kg of cake fertilizer are applied to each mu of land. Ploughing by 25-30 cm, ploughing finely and leveling to form a high furrow with the width of 1.3-1.5 m and the height of 20-30 cm, and making the furrow width of 40 cm; or making into small high ridges with the height of 15 cm and the width of 60 cm, and the width of the furrow is about 30 cm. Interplanting zedoary and corn field without additional soil preparation, cultivating 27-33 cm in the last ten days of 4 months or after harvesting, raking and leveling, making 1.3-1.65 m wide furrows, 40-47 cm in furrow grooves and 30-33 cm in furrow height when planting in fallow land and wheat stubble land.
Wen Yu jin (Wen Yu jin) is not suitable for deep ploughing and is contraindicated for continuous cropping, and the former crops are taro, carrot, rape, potato, dahurian angelica root, barley, wheat, etc. Generally, the soil is turned over for 20-27 cm, and then the soil is turned over once again to loosen the surface soil and make the lower layer compact, so that the turmeric cannot be drilled down for harvesting. The border making can be divided into narrow border and wide border: the width of the narrow ridge surface is 67 cm, and 1 row is planted; the width of the wide ridge is 1 meter, and 2 rows are planted. The width of each ditch is about 33 cm, the depth of each ditch is about 20 cm, the ridge surface is flat, and the ditch bottom is straight, so that the drainage is facilitated.
The traditional zedoary planting process is troublesome, the growth speed is slow, and the large-area planting of the zedoary is not facilitated. The curcuma zedoary which is rapidly propagated in the form of tissue culture seedlings is simple to plant, high in survival rate and high in later-stage growth speed. However, the existing curcuma zedoaria tissue culture technology is improved to a certain extent, but certain defects still exist in the aspect of preparation of a curcuma zedoaria tissue culture seedling culture medium, and the development of the curcuma zedoaria tissue culture seedling culture medium has important significance, and the scheme is generated.
Disclosure of Invention
The culture medium for the curcuma zedoary tissue culture seedlings and the preparation method thereof provided by the invention have the advantages that the survival rate of the curcuma zedoary tissue culture seedlings cultured by the culture medium is more than 90%, the period is short, the pollution is small, the production cost is low, and the large-scale planting is facilitated.
The specific technical scheme of the invention is as follows:
a culture medium for a curcuma zedoaria tissue culture seedling comprises the following components in parts by mass:
8g of lactose;
5g of ox gall salt;
0.2mg of 6-benzylaminopurine;
0.4mg indolebutyric acid;
30g of sucrose;
6g of agar powder;
5g of sodium chloride;
1000ml of water;
the manufacturing method comprises the following steps: mixing the above components except lactose, ox bile salt, 6-benzylaminopurine, indolebutyric acid, sucrose and agar, heating to dissolve, adjusting pH to 5.8 + -0.2 after sterilization, adding agar, 6-benzylaminopurine and indolebutyric acid, heating to melt, adding the rest components, shaking, packaging, sterilizing with deg.C high pressure steam for 15 min, cooling to 60 deg.C, and pouring into a flat dish.
The main function of 6-benzylaminopurine is to promote the formation of buds and also to induce the generation of callus. Belongs to a broad-spectrum plant growth regulator, can promote the growth of plant cells, inhibit the degradation of plant chlorophyll, improve the content of amino acid, delay the aging of leaves and the like, can be used for growing mung bean sprouts and soybean sprouts, and has the maximum use amount of 0.01g/kg and the residual amount of less than 0.2 mg/kg. Inducing bud differentiation, promoting lateral bud growth, promoting cell division, reducing decomposition of chlorophyll in plant, and inhibiting aging and keeping green.
The pure product of the indolebutyric acid is a white crystalline solid, and the original drug is white to light yellow crystals. Is a plant main root growth promoter, is commonly used for root soaking transplantation and hard branch pole insertion of woody and herbaceous plants, can accelerate the growth of roots and improve the rooting percentage of the plants, and can also be used for seed soaking and seed dressing of plant seeds and improve the germination rate and the survival rate. When the root soaking transplantation is carried out, the herbaceous plants are soaked in the solution with the concentration of 10-20 mg/L and the woody plants with the concentration of 50 mg/L; the dipping concentration of the inserted rod is 50-100 mg/L; the seed soaking and dressing concentration is 100mg/L (woody plant) and 10-20 mg/L (herbaceous plant). Indolebutyric acid also promotes the proliferation of tissue culture seedlings.
The outstanding substantive features and remarkable progress of the invention are as follows:
1. the operation is simple: the culture medium is optimized, so that the cluster buds can propagate in a large amount in a short time;
2. the curcuma zedoary tissue culture seedling is subjected to tissue culture by adopting the culture medium, the survival rate is over 90 percent, the period is short, the pollution is little, the production cost is low, and the large-scale planting is facilitated.
Detailed Description
The following detailed description of the preferred embodiments of the invention, while indicating the advantages and features of the invention, will become apparent to those skilled in the art from the following detailed description, which is by way of illustration, specific embodiments of the invention are described.
A culture medium for a curcuma zedoaria tissue culture seedling comprises the following components in parts by mass:
8g of lactose;
5g of ox gall salt;
0.2mg of 6-benzylaminopurine;
0.4mg indolebutyric acid;
30g of sucrose;
6g of agar powder;
5g of sodium chloride;
1000ml of water;
the manufacturing method comprises the following steps: mixing the above components except lactose, ox bile salt, 6-benzylaminopurine, indolebutyric acid, sucrose and agar, heating to dissolve, adjusting pH to 5.8 + -0.2 after sterilization, adding agar, 6-benzylaminopurine and indolebutyric acid, heating to melt, adding the rest components, shaking, packaging, sterilizing with deg.C high pressure steam for 15 min, cooling to 60 deg.C, and pouring into a flat dish.
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention is described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or some technical features of the present invention may be substituted. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (2)
1. A culture medium for a curcuma zedoary tissue culture seedling is characterized in that the components and the mass of the culture medium are respectively as follows:
8g of lactose;
5g of ox gall salt;
0.2mg of 6-benzylaminopurine;
0.4mg indolebutyric acid;
30g of sucrose;
6g of agar powder;
5g of sodium chloride;
1000ml of water.
2. The culture medium for the zedoary tissue culture seedling according to claim 1, wherein the preparation method comprises the following steps: mixing the above components except lactose, ox bile salt, 6-benzylaminopurine, indolebutyric acid, sucrose and agar, heating to dissolve, adjusting pH to 5.8 + -0.2 after sterilization, adding agar, 6-benzylaminopurine and indolebutyric acid, heating to melt, adding the rest components, shaking, packaging, sterilizing with deg.C high pressure steam for 15 min, cooling to 60 deg.C, and pouring into a flat dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911114139.8A CN110915651A (en) | 2019-11-14 | 2019-11-14 | Culture medium for curcuma zedoary tissue culture seedlings and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911114139.8A CN110915651A (en) | 2019-11-14 | 2019-11-14 | Culture medium for curcuma zedoary tissue culture seedlings and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110915651A true CN110915651A (en) | 2020-03-27 |
Family
ID=69853837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911114139.8A Pending CN110915651A (en) | 2019-11-14 | 2019-11-14 | Culture medium for curcuma zedoary tissue culture seedlings and preparation method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN110915651A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013084209A2 (en) * | 2011-12-09 | 2013-06-13 | Manuel Gidekel | Production of deschampsia antarctica in bioreactors |
| CN103820373A (en) * | 2014-03-19 | 2014-05-28 | 中国检验检疫科学研究院 | Chromogenic medium for separating and detecting enterobacter cloacae |
| CN104206277A (en) * | 2014-09-12 | 2014-12-17 | 南京通泽农业科技有限公司 | Rapid propagation method for curcuma zedoaria |
| CN108464243A (en) * | 2018-06-29 | 2018-08-31 | 钦州市农业科学研究所 | Curcuma zedoary tissue-culturing rapid propagation culture medium |
-
2019
- 2019-11-14 CN CN201911114139.8A patent/CN110915651A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013084209A2 (en) * | 2011-12-09 | 2013-06-13 | Manuel Gidekel | Production of deschampsia antarctica in bioreactors |
| CN103820373A (en) * | 2014-03-19 | 2014-05-28 | 中国检验检疫科学研究院 | Chromogenic medium for separating and detecting enterobacter cloacae |
| CN104206277A (en) * | 2014-09-12 | 2014-12-17 | 南京通泽农业科技有限公司 | Rapid propagation method for curcuma zedoaria |
| CN108464243A (en) * | 2018-06-29 | 2018-08-31 | 钦州市农业科学研究所 | Curcuma zedoary tissue-culturing rapid propagation culture medium |
Non-Patent Citations (1)
| Title |
|---|
| 杨世萍等: ""中药莪术组织培养研究进展"", 《亚太传统医药》 * |
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Application publication date: 20200327 |