CN104206277A - Rapid propagation method for curcuma zedoaria - Google Patents
Rapid propagation method for curcuma zedoaria Download PDFInfo
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- CN104206277A CN104206277A CN201410463184.5A CN201410463184A CN104206277A CN 104206277 A CN104206277 A CN 104206277A CN 201410463184 A CN201410463184 A CN 201410463184A CN 104206277 A CN104206277 A CN 104206277A
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Abstract
The invention provides a rapid propagation method for curcuma zedoaria. The rapid propagation method comprises the following steps: disinfecting leaves, inducing cluster buds, propagating the cluster buds, carrying out rooting culture, domesticating seedlings, transplanting and the like. The curcuma zedoaria prepared by the method has high survival rate, low production cost, short growth period and good hereditary stability and large-scale popularization and planting are facilitated.
Description
Technical field
The present invention relates to the quick-breeding method of curcuma zedoary tissue cultures, belong to plant technology field.
Background technology
Curcuma zedoary, Zingiber,
curcuma zedoaria (Christm.) Rosc, also known as kaempferia pundurata, radix zedoariae, smelly dung ginger, curcuma zedoary is Zingiber, turmeric perennial root draft, plant height about 1 meter; Rhizome is cylindrical, meat, fragrance as tool camphor, faint yellow or white; Elongated or the end of root enlarges into block root.Cultivation or wild under Lin Yin, there are distribution in the many ground of China, India to Malaysia.Produce the provinces and regions such as TaiWan, China, Fujian, Jiangxi, Guangdong, Guangxi, Sichuan, Yunnan, Anhui; Also there is distribution in India to Malaysia.Curcuma zedoary rhizome is containing volatile oil, and in oil, principal component is that curcuma zedoary furans ketenes accounts for 44.93%, and borneol accounts for 4.28%, Germacrone accounts for 6.16%, also containing α-and nopinene, and lauro lene, citrene, l, 8-press foline, terpinene, isoborneol, cloves alkene, curcumene, cloves alkene epoxides, turmerone, ar-turmerone, curdione and Curcumenol, isocurcumenol etc.Another containing two furans curcuma zedoary ketenes, curcumadiol.Again containing the curcumin chemical compounds of antioxidant activity.Broken blood gas, disappear long-pending pain relieving.For abdominal pain due to blood stasis, hepatosplenomegaly, trusted subordinate's distending pain, gather, women's blood stasis is through closing, and traumatic injury is had a pain eating accumulation.Cultivation or wild under Lin Yin, curcuma zedoary requires relatively stricter to ecotope, suitable planting area stenosis, and the main rhizome that adopts is planted, and survival rate is low, and tissue culture method survival rate is high, and production cost is low, and growth cycle is short, is beneficial to large-scale promotion plantation.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of curcuma zedoary, and high by the method preparation-obtained curcuma zedoary survival rate, production cost is low, and growth cycle is short, and genetic stability is good, is beneficial to large-scale promotion plantation.
Technical problem to be solved by this invention is realized by following scheme:
Get the blade of curcuma zedoary band petiole, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 35min, on superclean bench, 0.1% mercuric chloride disinfects 7min, drip two Tween-80s, aseptic water washing 5-7 time, the blade access culture medium prescription of the curcuma zedoary band petiole that sterilization treatment is crossed is for carrying out bud inducement cultivation in improvement WPM+NAA0.1mg/L+TDZ0.05mg/L+ zeatin 2mg/L inducing culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 1000-2000lx, temperature 25 ± 5 DEG C, the bud derived is put into medium improvement WPM+IBA0.01mg/L+6-BA1mg/L+ zeatin 1mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 2500lx, temperature 25 ± 5 DEG C, Multiplying culture Multiple Buds out puts into root media improvement WPM+NAA1.0mg/L+200mg/L active carbon+sucrose 15g/L+ agar 4.0mg/L, pH5.85, illumination 3500lx, temperature 25 ± 5 DEG C, test-tube plantlet is about 5cm, take out from blake bottle, plant in the nutritive cube of 10 × 10cm, nutritive cube matrix is through high-temperature sterilization and is added with the muck of organic manure, illumination 10000lx, temperature 25 ± 5 DEG C, humidity 80%, regularly water, fertilising, survival rate is added up after 30 days.
The curcuma zedoary survival rate adopting the present invention to prepare is 89%, and the cycle is short, and pollute little, production cost is low, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Get the blade of curcuma zedoary band petiole, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 35min, on superclean bench, 0.1% mercuric chloride disinfects 7min, drip two Tween-80s, aseptic water washing 5-7 time, the blade access culture medium prescription of the curcuma zedoary band petiole that sterilization treatment is crossed is for carrying out bud inducement cultivation in improvement WPM+NAA0.1mg/L+TDZ0.05mg/L+ zeatin 0.5mg/L inducing culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 1000-2000lx, temperature 25 ± 5 DEG C, the bud derived is put into medium improvement WPM+IBA0.01mg/L+6-BA0.8mg/L+ zeatin 1mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 2500lx, temperature 25 ± 5 DEG C, Multiplying culture Multiple Buds out puts into root media improvement WPM+NAA1.0mg/L+200mg/L active carbon+sucrose 15g/L+ agar 4.0mg/L, pH5.85, illumination 3500lx, temperature 25 ± 5 DEG C, test-tube plantlet is about 5cm, take out from blake bottle, plant in the nutritive cube of 10 × 10cm, nutritive cube matrix is through high-temperature sterilization and is added with the muck of organic manure, illumination 10000lx, temperature 25 ± 5 DEG C, humidity 80%, regularly water, fertilising, within 30 days, add up survival rate 84% afterwards.
Embodiment 2
Get the blade of curcuma zedoary band petiole, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 35min, on superclean bench, 0.1% mercuric chloride disinfects 7min, drip two Tween-80s, aseptic water washing 5-7 time, the blade access culture medium prescription of the curcuma zedoary band petiole that sterilization treatment is crossed is for carrying out bud inducement cultivation in improvement WPM+NAA0.1mg/L+TDZ0.05mg/L+ zeatin 2.5mg/L inducing culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 1000-2000lx, temperature 25 ± 5 DEG C, the bud derived is put into medium improvement WPM+IBA0.01mg/L+6-BA1.2mg/L+ zeatin 1mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 2500lx, temperature 25 ± 5 DEG C, Multiplying culture Multiple Buds out puts into root media improvement WPM+NAA1.0mg/L+200mg/L active carbon+sucrose 15g/L+ agar 4.0mg/L, pH5.85, illumination 3500lx, temperature 25 ± 5 DEG C, test-tube plantlet is about 5cm, take out from blake bottle, plant in the nutritive cube of 10 × 10cm, nutritive cube matrix is through high-temperature sterilization and is added with the muck of organic manure, illumination 10000lx, temperature 25 ± 5 DEG C, humidity 80%, regularly water, fertilising, within 30 days, add up survival rate 85% afterwards.
Embodiment 3
Get the blade of curcuma zedoary band petiole, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 35min, on superclean bench, 0.1% mercuric chloride disinfects 7min, drip two Tween-80s, aseptic water washing 5-7 time, the blade access culture medium prescription of the curcuma zedoary band petiole that sterilization treatment is crossed is for carrying out bud inducement cultivation in improvement WPM+NAA0.1mg/L+TDZ0.05mg/L+ zeatin 2.5mg/L inducing culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 1000-2000lx, temperature 25 ± 5 DEG C, the bud derived is put into medium improvement WPM+IBA0.01mg/L+6-BA1.0mg/L+ zeatin 1mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 2500lx, temperature 25 ± 5 DEG C, Multiplying culture Multiple Buds out puts into root media improvement WPM+NAA1.0mg/L+200mg/L active carbon+sucrose 15g/L+ agar 4.0mg/L, pH5.85, illumination 3500lx, temperature 25 ± 5 DEG C, test-tube plantlet is about 5cm, take out from blake bottle, plant in the nutritive cube of 10 × 10cm, nutritive cube matrix is through high-temperature sterilization and is added with the muck of organic manure, illumination 10000lx, temperature 25 ± 5 DEG C, humidity 80%, regularly water, fertilising, within 30 days, add up survival rate 85% afterwards.
Claims (5)
1. a method for quickly breeding for curcuma zedoary, comprise the sterilization of blade, the induction of Multiple Buds, the propagation of Multiple Buds, culture of rootage, acclimatization and transplants, its key step is as follows:
(1) blade of curcuma zedoary band petiole is got, to its disinfection;
(2) the blade access culture medium prescription of the curcuma zedoary band petiole that step (1) sterilization treatment is crossed is got for carrying out bud inducement cultivation in improvement WPM+NAA0.1mg/L+TDZ0.05mg/L+ zeatin 0.5-2.5mg/L inducing culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 1000-2000lx, temperature 25 ± 5 DEG C;
(3) get bud that step (2) derives to put into medium improvement WPM+IBA0.01mg/L+6-BA0.8-1.2mg/L+ zeatin 1mg/L and carry out Multiplying culture, additional saccharose 30g/L, agar 7g/L, pH5.85, illumination 2500lx, temperature 25 ± 5 DEG C;
(4) get step (3) Multiplying culture Multiple Buds out and put into root media improvement WPM+NAA1.0mg/L+200mg/L active carbon+sucrose 15g/L+ agar 4.0mg/L, pH5.85, illumination 3500lx, temperature 25 ± 5 DEG C;
(5) the curcuma zedoary test-tube plantlet after taking root carries out field acclimatization and transplants.
2. according to the method for quickly breeding of a kind of curcuma zedoary according to claim 1, it is characterized in that: the acquisition of the aseptic blade of curcuma zedoary described in step (1) is, get the blade of curcuma zedoary band petiole, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 35min, and on superclean bench, 0.1% mercuric chloride disinfects 7min, drip two Tween-80s, aseptic water washing 5-7 time.
3., according to the method for quickly breeding of a kind of curcuma zedoary according to claim 1, it is characterized in that: step (2) (3) (4) improvement WPM be nitric acid ammonia reduce to original 3/4, calcareous material increases original 1/4.
4. according to the method for quickly breeding of a kind of curcuma zedoary according to claim 1, it is characterized in that: the zeatin adding certain content in step (2) and (3) all has facilitation to the induction of curcuma zedoary Multiple Buds and propagation.
5. according to the method for quickly breeding of a kind of curcuma zedoary according to claim 1, it is characterized in that: the acclimatization and transplants method in step (5) is that test-tube plantlet is about 5cm, take out from blake bottle, plant in the nutritive cube of 10 × 10cm, nutritive cube matrix is through high-temperature sterilization and is added with the muck of organic manure, illumination 10000lx, temperature 25 ± 5 DEG C, humidity 80%, regularly waters, fertilising, added up survival rate after 30 days.
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| CN201410463184.5A CN104206277A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for curcuma zedoaria |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557513A (en) * | 2015-12-11 | 2016-05-11 | 广西壮族自治区药用植物园 | Tissue culture and rapid propagation method of curcuma longa |
| CN106718877A (en) * | 2016-11-23 | 2017-05-31 | 中国科学院华南植物园 | A kind of flourishing torch root tuber of aromatic turmeric high quality seedling rapid propagation method |
| CN110915651A (en) * | 2019-11-14 | 2020-03-27 | 黄志君 | Culture medium for curcuma zedoary tissue culture seedlings and preparation method thereof |
| CN112470936A (en) * | 2020-12-25 | 2021-03-12 | 广西壮族自治区农业科学院 | Method for forming curcuma aromatica in Guangxi curcuma zedoary tissue culture seedling bottle |
| CN113966717A (en) * | 2021-11-10 | 2022-01-25 | 广西壮族自治区药用植物园 | Method for transplanting large curcuma zedoary tissue culture seedlings in greenhouse |
-
2014
- 2014-09-12 CN CN201410463184.5A patent/CN104206277A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557513A (en) * | 2015-12-11 | 2016-05-11 | 广西壮族自治区药用植物园 | Tissue culture and rapid propagation method of curcuma longa |
| CN106718877A (en) * | 2016-11-23 | 2017-05-31 | 中国科学院华南植物园 | A kind of flourishing torch root tuber of aromatic turmeric high quality seedling rapid propagation method |
| CN106718877B (en) * | 2016-11-23 | 2019-06-21 | 中国科学院华南植物园 | A kind of flourishing torch Radix Curcumae high quality seedling rapid propagation method |
| CN110915651A (en) * | 2019-11-14 | 2020-03-27 | 黄志君 | Culture medium for curcuma zedoary tissue culture seedlings and preparation method thereof |
| CN112470936A (en) * | 2020-12-25 | 2021-03-12 | 广西壮族自治区农业科学院 | Method for forming curcuma aromatica in Guangxi curcuma zedoary tissue culture seedling bottle |
| CN112470936B (en) * | 2020-12-25 | 2021-06-22 | 广西壮族自治区农业科学院 | Method for forming curcuma aromatica in Guangxi curcuma zedoary tissue culture seedling bottle |
| CN113966717A (en) * | 2021-11-10 | 2022-01-25 | 广西壮族自治区药用植物园 | Method for transplanting large curcuma zedoary tissue culture seedlings in greenhouse |
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Application publication date: 20141217 |