CN106069789A - One is carried disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture - Google Patents
One is carried disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture Download PDFInfo
- Publication number
- CN106069789A CN106069789A CN201610693912.0A CN201610693912A CN106069789A CN 106069789 A CN106069789 A CN 106069789A CN 201610693912 A CN201610693912 A CN 201610693912A CN 106069789 A CN106069789 A CN 106069789A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- disease germs
- vitro
- tissue culture
- vegetable material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses one to carry disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture.The in vitro vegetable material tissue culture medium (TCM) that carries disease germs of the present invention, including vegetable solid culture medium and the cephalosporin solution that concentration is 2000 2500mg/L that is positioned at its upper strata.During training at actual group, the in vitro vegetable material that will carry disease germs is inoculated in vegetable solid culture medium, then cephalosporin solution is added in vegetable solid media surface, carry out tissue culture again, this training method avoids the toxic and side effects to isolated culture material reaching Efficient antibacterial simultaneously, bottleneck problem in the tissue cultured seedling production process of the vegetable material successfully solving band antibacterial, efficiently accomplishes propagation, rooting process, produces required plant tissue culture Seedling；The method can apply to the isolated culture vegetable material of multiple band antibacterial, and antibacterial efficiency is high, and production safety is reliable, has strong operability, production efficiency advantages of higher, has a extensive future.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to one and carry disease germs in vitro vegetable material tissue culture medium (TCM)
And method for tissue culture.
Background technology
Multiple fields that plant tissue culture is widely used in agricultural production, at seedling detoxification production, seedling
The aspects such as Fast-propagation, the scale application of plant new resources, new variety of plant selection-breeding create far-reaching shadow to modern agriculture
Ring, be agricultural biotechnologies models in modern agriculture application, be the modern important component part planting industry.At present, China plants
Thing tissue cultured seedling achieves large-area popularization and application in flowers, fruit tree, shade plant, medicinal plants etc., and the whole world is planted
Thing tissue cultured seedling year the volume of trade more than 15,000,000,000 dollars, and, every year with the speed more than 10% be incremented by.Utilize based on from
The plant seedling tissue culture and rapid propagation method of body culture technique produces seedling, than Sterile culture method fast ten thousand times or even 100,000 times with
On, a plant can breed several ten thousand for 1 year to millions of plant.Utilize group culturation rapid propagating technology non-to the cost breeding seedling
The cheapest, lower more than 10 times than with the seeling industry method production cost of routine.Further, low to some coefficients of nourishing and generating and not
Can be by the agricultural plant (such as Fructus Musae etc.) of seminal propagation or seed is difficult to sprout under field conditions (factors) plant (such as iron sheet stone
Dry measure used in former times etc.) or rare numbers excellent material (as in production process find some excellent variation individual plant etc.) for, without
Group culturation rapid propagating technology, it is extremely difficult for wanting realize its scale industrialization to produce, agriculture when being bred some by seed
For skill character there will be the crop (such as Fructus Caricae etc.) of separation, without seedling group train detoxifying fast breeding technique, want into
It is also extremely difficult that one step improves its productivity effect.It is based on seedling group culturation rapid propagating technology in reproduction speed height and low cost
The advantage of the aspect such as honest and clean, plant seedling tissue-culturing rapid propagation has been developed into a huge industry at agriculture field at present, and obtains
Huge economic results in society.The seedling tissue-culturing rapid propagation industry annual value of production of our province and technology maintain the leading position in the whole nation, mesh
Front our province relates to utilize tissue-culturing rapid propagation to have kind more than 300 to the agricultural plant producing seedling, and estimation group training industry annual value of production is 10
More than hundred million yuan.
At present in tissue cultured seedling production process, pollution rate is higher, some plant brownization serious, seedling vitrification and aberration rate
The problems such as height the most seriously exist in many seeling industry enterprises, and the existence of these problems seriously hinders tissue cultured seedling production cost
Further reduction.Particularly a lot of tissue cultured seedling manufacturing enterprises are after production for many years, owing to production environment is deteriorated and raw
Product instrument is aging waits cause influence, and the probability of happening of germ contamination is greatly increased.
At present, domestic existing the antibacterial report adding antibiotic in culture medium, this side are gone in culture medium high temperature sterilize
Method one is, through high-temperature sterilization, its antibacterial power is had certain destruction, and the antibiotic being simultaneously dissolved in culture medium is to isolated culture material
The toxic and side effects that material produces is bigger.Controlling to pollute in plant tissue culture Seedling production process is one of its key technology, carefully
The probability of happening that bacterium pollutes is higher, causes manufacturing enterprise's high cost or even failure.Therefore, exploitation is a kind of in vitro plant material
Can be the most antibacterial and culture materials is not produced the tissue of toxic and side effects (or minimum toxic and side effects) in material tissue cultured seedling production process
Culture medium and method for tissue culture are particularly important.
Yet there are no first by the aseptic cephalosporin solution of medical cephalosporin compounding high concentration, under aseptic condition
Add the formation biphase training method of solid-liquid in media surface to avoid isolated culture material to reach Efficient antibacterial simultaneously
The report of toxic and side effects and patent application.
Summary of the invention
It is an object of the invention to solve because occurring the vegetable material after band antibacterial not only to expand germ contamination scope but also
Cause the unavailable problem of germ contamination material, develop during one effectively produces for tissue cultured seedling and have occurred and that band antibacterial
In vitro plant culturing material utilize with remaining valid its propagation, tissue cultured seedling of having taken root produce tissue culture medium (TCM) and tissue training
Breeding method.
First purpose of the present invention is to provide one and carries disease germs in vitro vegetable material tissue culture medium (TCM), including, vegetable solid
Culture medium and be positioned at the cephalosporin solution on its upper strata.
Preferably, the concentration of described cephalosporin solution is 2000-2500mg/L.
Preferably, the thickness that described cephalosporin solution is formed on vegetable solid culture medium upper strata is 1-2mm.
Preferably, medical cephalosporin is dissolved in sterilized water and prepares by described cephalosporin solution.
Preferably, described vegetable solid culture medium is for plant tissue propagation or the solid medium of root culture.
Preferably, described plant is Herba Dendrobii, Herba Anoectochili roxburghii, Fructus Musae, Fructus Caricae, the white palm or climing green floss.
Second object of the present invention is to provide one and carries disease germs in vitro vegetable material method for tissue culture, will carry disease germs and plant in vitro
Thing material is inoculated in vegetable solid culture medium, then adds cephalosporin solution on vegetable solid culture medium upper strata, then carries out
Tissue culture.
Preferably, described cephalosporin solution concentration is 2000-2500mg/L, and described cephalosporin solution is solid plant
The thickness that body culture medium upper strata is formed is 1-2mm.
Third object of the present invention is to provide the described in vitro vegetable material tissue culture medium (TCM) that carries disease germs and plants in vitro carrying disease germs
Application in the cultivation of thing material.
Preferably, the described in vitro vegetable material tissue culture medium (TCM) that carries disease germs is in vitro vegetable material propagation, the root culture of carrying disease germs
In application.
The present invention selects medical cephalosporin injection to be the antibiotic used, and is configured to it highly concentrated under aseptic condition
The aseptic cephalosporin solution of degree (2000-2500mg/L), is added to planting of sterilizing cooled and solidified under aseptic condition
On thing solid culture primary surface, define the biphase training method of solid-liquid and avoid isolated culture material to reach Efficient antibacterial simultaneously
The toxic and side effects of material, bottleneck problem in the tissue cultured seedling production process of the vegetable material successfully solving band antibacterial, efficiently accomplish
Propagation, rooting process, produce required plant tissue culture Seedling.The present invention utilizes the aseptic cephalosporin solution of high concentration, permissible
It is applied to the isolated culture vegetable material of multiple band antibacterial, can be extremely low to isolated culture material propagation and growth effect of taking root
Under conditions of complete its tissue cultured seedling factorial praluction process, antibacterial efficiency is high, and production safety is reliable, particularly to some economic valencys
The tissue cultured seedling of the material that carries disease germs of the orchid that value is high produces largely effective, and has strong operability, and it is excellent that production efficiency is high etc.
Point, has a extensive future.
Detailed description of the invention
Following example are to further illustrate the present invention rather than limitation of the present invention.
Embodiment 1
1. material: the experiment material of this embodiment is Herba Dendrobii (the Dendrobium officinale carried disease germs
Kimura et Migo) isolated culture material.
Herba Dendrobii is that China commonly uses rare Chinese medicine, excavates due to the most immoderate, and breeding potential the most own is low, natural
Resource exhaustion, now for the medical material kind of special-protection-by-the-State.There is nourishing YIN and clearing away heat.Promote the production of body fluid the effects such as stomach reinforcing, nourishing the lung to arrest cough, be used for
Consumption of body fluid caused by febrile disease, xerostomia is fidgety, after being ill the various disease conditions such as deficiency-heat.Modern pharmacological research shows, Herba Dendrobii also has antitumor, anti-ageing
Always, body immunity and the effect of expansion blood vessel are strengthened.Herba Dendrobii originates in district and is mostly in temperate zone and subtropical zone, annual weather
Warm, moistening, winter temperature is more than 0 DEG C.When carrying out the implantation in large scale of Herba Dendrobii, be according to the growth of Herba Dendrobii
Habit, it is considered to the multiple natural causes such as the illumination in place, temperature, humidity, ventilation carry out precision management.The rule of Herba Dendrobii at present
The seedling that modelling produces is coming substantially from aseptic seeding.
The preparation of the most aseptic cephalosporin solution
Buy the some bottles of cephalosporin injection (every bottle of 1g dress) of medical injection.Select band suction pipe 250mL drop bottle and
The graduated cylinder of 1000mL cleans up, in 1.06kg/cm after being packaged with newspaper after drying2Sterilizing under the conditions of (121 DEG C)
The Cymbidium ensifolium (L.) Sw. bottle of 20min, another 500mL injects the deionized water of 300mL, in 1.06kg/cm2Sterilizing under the conditions of (121 DEG C)
30min, it is thus achieved that sterilized water.Above medicine, utensil, sterilized water are positioned on superclean bench, are configured under aseptic condition
The aseptic cephalosporin solution of 2500mg/L, and this aseptic cephalosporin solution is sub-packed in aseptic drop bottle, use aseptic cattle
Mulberry paper is stand-by by preserving under 4 DEG C of refrigerated conditions of placement after the neck part sealing of drop bottle.
3. culture medium preparation
The requirement produced according to culture materials candidum tissue culturing seedling, prepares its proliferated culture medium PB3 and training of taking root respectively
Support base
PR2.Concrete formula is: proliferated culture medium PB3: spend precious No. 1 1.5g/L+ to spend precious No. 2 1.5g/L+ coconut milk 200mL/
L+ activated carbon 2g/L+6-BA 2.0mg/L+NAA 0.2mg/L+ sucrose 15g/L+ agar 6g/L；Root media PR2: spend precious 1
Number 1g/L+ spends precious No. 2 1g/L+ peptone 2g/L+NAA1.0mg/L+ activated carbon 2g/L+ volume fraction 10% (final concentration) Fructus Musaes
Juice+sucrose 15g/L+ agar 6g/L.It is 5.4 that both the above culture medium all adjusts pH, then in 1.06kg/cm2(121 DEG C) condition
Lower sterilizing 20min, cools down stand-by.
Spend precious No. 1 (HYPONeX 1) that used is TaiWan, China platform produced in USA and gardening enterprise stock action
Company limited's subpackage product.Spending precious No. 2 (HYPONeX 2) is U.S.'s Haponex Products, its N:P:K=20:20:20.
4. the material that carries disease germs inoculation operation and cultivation
Being taken out from refrigerator by aseptic cephalosporin solution places superclean bench, the ferrum carried disease germs by normal operation cutting
Skin Herba Dendrobii isolated culture material, transplanting, on proliferated culture medium PB3, then injects 5mL toward proliferated culture medium PB3 surface (upper strata)
Aseptic cephalosporin solution, this solution layer thickness is about 1mm, defines the state that solid-liquid is biphase, finally covers bottle cap, complete
Inoculation operation.Enrichment culture condition be cultivation temperature be (25 ± 2) DEG C, intensity of illumination 1 500-2 000Lx, illumination 12h/d.One
As within 45 days, be 1 subculture multiplication cycle, every 1 subculture multiplication week after date proliferation times be 2～4 times.When cultivating a subculture
All after dates, the antibacterial of Herba Dendrobii isolated culture material is significantly suppressed under the effect of cephalosporin solution, trains simultaneously
Propagation and the growth of supporting material are the most significantly affected.Again repeat above inoculation operation as differentiation material and add
Add cephalosporin solution in the surface of culture medium, cover bottle cap, cultivate；So circulation, can reach the group of usual sterilizable material
Seedlings cultivating production effect.
Cut the Herba Dendrobii isolated culture material carried disease germs of pending root culture by normal operation, transplanting is in taking root
In culture medium PR2, then inject the aseptic cephalosporin solution of 5mL, this solution thickness toward root media PR2 surface (upper strata)
Degree is about 1mm, defines the state that solid-liquid is biphase, finally covers bottle cap, completes inoculation operation.Rooting and hardening-off culture condition is training
Supporting temperature is (26 ± 2) DEG C, illuminance 2 000-3 000Lx, illumination 12h/d.The Rooting and hardening-off culture cycle is 60-90 days.Raw
Bud in root culture medium can normal long root and growth, until bottle outlet transplant.
5. tissue cultured seedling was transplanted: when tissue cultured seedling length of taking root is to 4～8 centimetres high, natural lighting lower refining seedling 10 days.Then beat
Corkage plug, takes out tissue cultured seedling from culture bottle with tweezers, washes root culture medium off, plant and become with sawdust mixed in equal amounts into by bark
Substrate in.Noting watering, shade, be incubated and moisturizing, survival rate is up to more than 95%.
Embodiment 2
1. material: the experiment material of this embodiment is Herba Anoectochili roxburghii (the Anoectochilus roxburghii carried disease germs
(wall) lindl.) isolated culture material.
Herba Anoectochili roxburghii another name Anoectochilus nefiliforme (Nakai) hara, Herba Melicae Scabrosae, be that the orchid family (Orchidaceae) opens lip plant flowers aspidistra genus
(Anoectochilus Bl.) perennial rare herbaceous plant, China has about 23 kinds, and wherein Some Species herb is among the people makees medicine
With, for traditional rare rare Chinese medicine, it is relatively wide in range among the people, is used for treating diabetes, hyperlipidemia, hepatitis B
Etc. disease, have " king of medicine ", " gold grass ", " god medicine ", the laudatory title such as " crow Radix Ginseng ", be referred to as " king in medicine ".
The preparation of the most aseptic cephalosporin solution
Buy the some bottles of cephalosporin injection (every bottle of 1g dress) of medical injection.Select band suction pipe 250mL drop bottle and
The graduated cylinder of 1000mL cleans up, in 1.06kg/cm after being packaged with newspaper after drying2Sterilizing under the conditions of (121 DEG C)
The Cymbidium ensifolium (L.) Sw. bottle of 20min, another 500mL injects the deionized water of 300mL, in 1.06kg/cm2Sterilizing under the conditions of (121 DEG C)
30min, it is thus achieved that sterilized water.Above medicine, utensil, sterilized water are positioned on superclean bench, are configured under aseptic condition
The aseptic cephalosporin solution of 2000mg/L, and this aseptic cephalosporin solution is sub-packed in aseptic drop bottle, use aseptic cattle
Mulberry paper is stand-by by preserving under 4 DEG C of refrigerated conditions of placement after the neck part sealing of drop bottle.
3. culture medium preparation
The requirement produced according to culture materials Herba Anoectochili roxburghii tissue cultured seedling, prepares its proliferated culture medium and root media respectively.
Concrete formula is: proliferated culture medium: MS+6-BA 5.0mg/L+NAA 0.5mg/L+ peptone 2g/L+ sucrose 30g/L+ agar
6g/L；Root media: MS+NAA1.0mg/L+ activated carbon 2g/L+ volume fraction 10% (final concentration) bananas juice+sucrose 30g/L
+ agar 6g/L.It is 5.4 that both the above culture medium all adjusts pH, then in 1.06kg/cm2Sterilizing 20min under the conditions of (121 DEG C),
Cool down stand-by.
4. the material that carries disease germs inoculation operation and cultivation
Being taken out from refrigerator by aseptic cephalosporin solution places superclean bench, the gold carried disease germs by normal operation cutting
Anoectochilus roxburghii isolated culture material, transplanting, on proliferated culture medium, then injects the pioneer of 10mL toward enrichment culture primary surface (upper strata)
Mould cellulose solution, this solution layer thickness is about 2mm, defines the state that solid-liquid is biphase, finally covers bottle cap, completes inoculation operation.
Enrichment culture condition be cultivation temperature be (23 ± 2) DEG C, intensity of illumination 1 500-2 000Lx, illumination 10h/d.General 45 days is 1
The individual subculture multiplication cycle, every 1 subculture multiplication week after date proliferation times be 3～5 times.After cultivating a subculture cycle, gold
The antibacterial of Anoectochilus roxburghii isolated culture material is significantly suppressed under the effect of cephalosporin solution, simultaneously the propagation of culture materials
The most significantly affected with growth.Again repeat above inoculation operation as differentiation material and interpolation cephalosporin is molten
Liquid, in the surface of culture medium, covers bottle cap, cultivates；So circulation, can reach the tissue cultured seedling production effect of usual sterilizable material.
Cut the Herba Anoectochili roxburghii isolated culture material carried disease germs of pending root culture by normal operation, transplanting is in training of taking root
Supporting on base, then inject the cephalosporin solution of 10mL toward root culture primary surface (upper strata), this solution layer thickness is about 2mm,
Define the state that solid-liquid is biphase, finally cover bottle cap, complete inoculation operation.Rooting and hardening-off culture condition is that cultivation temperature is
(25 ± 2) DEG C, illuminance 2 000-3 000Lx, illumination 10h/d.The Rooting and hardening-off culture cycle is 60 days.On root media
Bud can normal long root and growth, until bottle outlet transplant.
5. tissue cultured seedling was transplanted: when tissue cultured seedling length of taking root is to 5～8 centimetres high, natural lighting lower refining seedling 10 days.Then beat
Corkage plug, takes out tissue cultured seedling from culture bottle with tweezers, washes root culture medium off, plant into by peat soil and Vermiculitum mixed in equal amounts
In the substrate become, drench with 800 times of carbendazim after transplanting.Note watering, shade, be incubated and moisturizing, survival rate up to 95% with
On.
Embodiment 3
Use the method being similar in embodiment 1 and embodiment 2, by the aseptic cephalosporin solution of 2000-2500mg/L
For Fructus Musae, Fructus Caricae, the white palm or the isolated culture material carried disease germs of climing green floss, also it is attained by normal aseptic isolated culture
The propagation of material, root culture effect, the survival rate after tissue cultured seedling is transplanted all can reach more than 95%.
Claims (10)
1. the in vitro vegetable material tissue culture medium (TCM) that carries disease germs, it is characterised in that include vegetable solid culture medium and position thereon
The cephalosporin solution of layer.
The in vitro vegetable material tissue culture medium (TCM) that carries disease germs the most according to claim 1, it is characterised in that described cephalosporin
The concentration of solution is 2000-2500mg/L.
The in vitro vegetable material tissue culture medium (TCM) that carries disease germs the most according to claim 1, it is characterised in that described cephalosporin
The thickness that solution is formed on vegetable solid culture medium upper strata is 1-2mm.
The in vitro vegetable material tissue culture medium (TCM) that carries disease germs the most according to claim 1, it is characterised in that described cephalosporin
Medical cephalosporin is dissolved in sterilized water and prepares by solution.
The in vitro vegetable material tissue culture medium (TCM) that carries disease germs the most according to claim 1, it is characterised in that described vegetable solid
Culture medium is for plant tissue propagation or the solid medium of root culture.
The in vitro vegetable material tissue culture medium (TCM) that carries disease germs the most according to claim 1, it is characterised in that described plant is ferrum
Skin Herba Dendrobii, Herba Anoectochili roxburghii, Fructus Musae, Fructus Caricae, the white palm or climing green floss.
7. the in vitro vegetable material method for tissue culture that carries disease germs, it is characterised in that comprise the following steps: will carry disease germs in vitro plant
Material is inoculated in vegetable solid culture medium, then adds cephalosporin solution on vegetable solid culture medium upper strata, then carries out group
Knit cultivation.
The in vitro vegetable material method for tissue culture that carries disease germs the most according to claim 7, it is characterised in that described pioneer is mould
Cellulose solution concentration is 2000-2500mg/L, and the thickness that described cephalosporin solution is formed on vegetable solid culture medium upper strata is
1-2mm。
9. in vitro vegetable material tissue culture medium (TCM) the answering in the in vitro vegetable material that carries disease germs is cultivated of carrying disease germs described in claim 1
With.
Application the most according to claim 9, it is characterised in that the in vitro vegetable material tissue that carries disease germs described in claim 1
Culture medium application in carry disease germs in vitro vegetable material propagation, root culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610693912.0A CN106069789B (en) | 2016-08-19 | 2016-08-19 | One kind is carried disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610693912.0A CN106069789B (en) | 2016-08-19 | 2016-08-19 | One kind is carried disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106069789A true CN106069789A (en) | 2016-11-09 |
| CN106069789B CN106069789B (en) | 2018-09-28 |
Family
ID=58070663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610693912.0A Active CN106069789B (en) | 2016-08-19 | 2016-08-19 | One kind is carried disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106069789B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112335547A (en) * | 2020-11-11 | 2021-02-09 | 泉州市泉美生物科技有限公司 | Cultivation method of monarch tissue culture seedling micro-vase landscape |
| CN116349604A (en) * | 2023-05-12 | 2023-06-30 | 西昌海越生物科技有限公司 | Orange stem cranberry green velvet tissue culture method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100177A (en) * | 2010-11-19 | 2011-06-22 | 成都大学 | Efficient degerming method used during rapid production of bulbus fritillariae cirrhosae compost |
| CN102257966A (en) * | 2011-07-01 | 2011-11-30 | 山东省农业科学院蔬菜研究所 | Method for preventing pollution of garlic ovary culture |
| CN102986526A (en) * | 2011-11-02 | 2013-03-27 | 福建省亚热带植物研究所 | Open-type plant tissue culture method |
| CN104396735A (en) * | 2014-09-22 | 2015-03-11 | 广西壮族自治区药用植物园 | Method for eliminating bacterial contamination in Momordica grosvenori tissue culture |
| CN105815217A (en) * | 2016-03-23 | 2016-08-03 | 中国林业科学研究院热带林业研究所 | Treatment method for bacterial contamination in tissue-culture industrialized seedling culture of tropical woody plants |
-
2016
- 2016-08-19 CN CN201610693912.0A patent/CN106069789B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100177A (en) * | 2010-11-19 | 2011-06-22 | 成都大学 | Efficient degerming method used during rapid production of bulbus fritillariae cirrhosae compost |
| CN102257966A (en) * | 2011-07-01 | 2011-11-30 | 山东省农业科学院蔬菜研究所 | Method for preventing pollution of garlic ovary culture |
| CN102986526A (en) * | 2011-11-02 | 2013-03-27 | 福建省亚热带植物研究所 | Open-type plant tissue culture method |
| CN104396735A (en) * | 2014-09-22 | 2015-03-11 | 广西壮族自治区药用植物园 | Method for eliminating bacterial contamination in Momordica grosvenori tissue culture |
| CN105815217A (en) * | 2016-03-23 | 2016-08-03 | 中国林业科学研究院热带林业研究所 | Treatment method for bacterial contamination in tissue-culture industrialized seedling culture of tropical woody plants |
Non-Patent Citations (2)
| Title |
|---|
| 吴洪生: "植物组织培养中的染菌及预防", 《药物生物技术》 * |
| 邢东方等: "枣组织培养中抗生素对污染的抑制作用", 《经济林研究》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112335547A (en) * | 2020-11-11 | 2021-02-09 | 泉州市泉美生物科技有限公司 | Cultivation method of monarch tissue culture seedling micro-vase landscape |
| CN116349604A (en) * | 2023-05-12 | 2023-06-30 | 西昌海越生物科技有限公司 | Orange stem cranberry green velvet tissue culture method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106069789B (en) | 2018-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102835318B (en) | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition | |
| CN105340747A (en) | Asexual rapid propagation method for radix glycyrrhizae | |
| CN104106468B (en) | The quick breeding method for tissue culture of a kind of radix fici simplicissimae | |
| CN103348920B (en) | Rapid propagation method for high quality seedlings of Kyara | |
| CN105165617B (en) | The tissue cultures and fast seedling-cultivating method of blood aspidistra seed | |
| CN104429865A (en) | Sexual propagation method of bletilla striata | |
| CN103125396B (en) | Yam seedling in-vitro propagation method | |
| CN104082138A (en) | Tissue-culture rapid propagation method of Aristolochia fordiana Hemsl | |
| CN104663460A (en) | Tissue culture and rapid propagation method for epimedium wushanense | |
| CN105230489A (en) | Tissue culture fast propagation method for dendrobium candidum | |
| CN105191792B (en) | The rapid propagation method of almond ringdove chrysanthemum | |
| CN103270946B (en) | A kind of seedlings of Dendrobium officinale is produced and hardening synchronous method | |
| CN105265320A (en) | Tissue culture propagation method for herba aristolochia mollissima | |
| CN104488723A (en) | Tissue-culture and rapid-propagation method of epimedium koreanum nakai | |
| CN106069789B (en) | One kind is carried disease germs in vitro vegetable material tissue culture medium (TCM) and method for tissue culture | |
| CN104813931A (en) | Tissue culture and rapid propagation method for Dendrobium officinale | |
| CN105191803A (en) | Dendrobium officinale tissue culture bag seedling production method | |
| CN102613089B (en) | High-efficient in-vitro propagation method of 20-year-old schima superba big tree | |
| CN105210884A (en) | Bletilla seed asepsis sprouting equilibrated Medium | |
| CN106718942A (en) | The tissue-culturing rapid propagation and domestication culture techniques of North China's dendrobium candidum | |
| CN106416972A (en) | Method for quickly cultivating bletilla striata seedlings | |
| CN110663552A (en) | Tissue culture and rapid propagation method of Yunnan tung tree | |
| CN105432470A (en) | Tissue culture method for dendrobium moniliforme | |
| CN105284625A (en) | Novel seedling cultivating method of dendrobium candidum | |
| CN105123512A (en) | Breeding method of anoectochilus roxburghii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |