CN110915548A - Auricularia auricula bagged culture material additive and using method thereof - Google Patents
Auricularia auricula bagged culture material additive and using method thereof Download PDFInfo
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- CN110915548A CN110915548A CN201911277348.4A CN201911277348A CN110915548A CN 110915548 A CN110915548 A CN 110915548A CN 201911277348 A CN201911277348 A CN 201911277348A CN 110915548 A CN110915548 A CN 110915548A
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- China
- Prior art keywords
- agaric
- bagged
- culture material
- pyrroloquinoline quinone
- water
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an agaric bagged culture material additive and a preparation method of a using method thereof, wherein the preparation method of the culture material additive comprises the following steps: dissolving a certain amount of pyrroloquinoline quinone in water according to the weight ratio of 0.8-1.2: 1000, fully and uniformly mixing with bagged agaric culture material dry materials prepared by a traditional method according to the weight ratio of 1: 1, continuously replenishing water to meet the humidity requirement of the culture materials, and then continuously operating according to the traditional mode of culturing agaric by using the bagged culture materials.
Description
Technical Field
The invention relates to an agaric bagged culture material additive and a using method thereof, which are mainly applied to the technical field of microbial culture and edible fungus culture.
Background
The black fungus belongs to the medium-temperature edible fungi and is mainly distributed in the high mountain areas of temperate zone and subtropical zone. Black fungus is naturally distributed in China widely, is a precious edible and medicinal gummy fungus in China, and is a well-known health food in the world.
The basidiospores of the black fungus germinate into hyphae under the condition of proper conditions, tubular villus hyphae with only one nucleus in each transverse septum, namely mononuclear hyphae, are developed firstly, and then are fused mutually through the contact of top cells of the mononuclear hyphae with two different sexes to form a binuclear cell. At this time, hyphae continuously grow and develop to absorb a large amount of nutrition and moisture, colloid fruit body primordium is formed on the surface of the substrate when the conditions are mature, and then the fruit body is grown. The formation of primordia of fruiting bodies determines the quality and yield of Auricularia, and thus this process is very important. The formation of the fruiting body primordium needs a plurality of active substances such as protease and the like to participate, so that nitrogen in the culture material is fully utilized.
Pyrroloquinoline quinone is a coenzyme of oxidoreductase participating in respiratory chain, has high biological activity, and can influence hypha to produce high-activity protease.
A certain amount of pyrroloquinoline quinone is added into bagged culture materials of the agaric, so that a large number of fruiting body primordiums can be formed by agaric hyphae in advance, the production efficiency is improved, the agaric yield is increased, and the agaric quality is improved. The protein content in the agaric is one of important quality indexes of the agaric, the protein content is high, the nutrition is high, and the agaric has good taste.
Disclosure of Invention
The invention aims to promote the growth of agaric hyphae under a certain concentration by adopting a substance capable of influencing the growth of the agaric hyphae. The invention is realized by the following technical scheme:
the invention provides an agaric bagged culture material additive and a using method thereof, wherein the method comprises the following steps:
(1) adding a certain amount of pyrroloquinoline quinone into water, and fully dissolving to prepare pyrroloquinoline quinone solution.
(2) And fully and uniformly mixing the prepared bagged agaric culture material dry material and the pyrroloquinoline quinone solution according to a certain proportion, and continuously adding water to meet the humidity requirement of the culture material.
Preferably, in the step (1), a certain amount of pyrroloquinoline quinone is added into water in a weight ratio of 0.8-1.2 (pyrroloquinoline quinone) to 1000 (water).
Preferably, in the step (2), the prepared bagged agaric compost dry material and the pyrroloquinoline quinone solution are fully and uniformly mixed according to the weight ratio of 1: 1.
Compared with the prior art, the invention has the following advantages: a certain amount of pyrroloquinoline quinone is added into bagged culture materials of the agaric, so that a large number of fruiting body primordiums can be formed by agaric hyphae in advance, the production efficiency is improved, the agaric yield is increased, and the agaric quality is improved. The protein content in the agaric is one of important quality indexes of the agaric, the protein content is high, the nutrition is high, and the agaric has good taste
Drawings
FIG. 1 shows the effect of pyrroloquinoline quinone at different concentrations on the yield and protein content of Auricularia.
Detailed Description
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
Example 1
The bagged agaric cultivation material is prepared with sawdust 77.6 wt%, bran 20 wt%, cane sugar 1 wt%, gypsum 1 wt% and urea 0.4 wt%, and through mixing with pyrroloquinoline quinone solution in the weight ratio of 1 to 1, adding pyrroloquinoline quinone solution in the amount of 0 g, 0.6 g, 0.8 g, 1 g, 1.2 g and 1.4 g, mixing and adding water until there is no water mark in finger seam. Packaging the above materials in 200 bags according to the same specification, sterilizing and culturing according to the same method, collecting Auricularia, and calculating Auricularia dry weight and protein content.
Note: the sample is the content of pyrroloquinoline quinone (g/kg)
Compared with the control, when the content of pyrroloquinoline quinone is 0.8-1.2 g/kg, the growth speed of hyphae is accelerated, and the kilogram number of dry agaric produced by 100 kg of dry materials and the protein content are obviously increased. When the content of the pyrroloquinoline quinone is 1.0 g/kg, the yield is increased by (10.1-8.8)/8.8 by 14.8 percent compared with the control; the protein content increase is (11.9-10.8)/10.8 is 10.2%
Example 2
The agaric cultivation material is prepared by using 93% of cottonseed hulls, 1% of cane sugar, 5% of bran and 1% of gypsum powder, fully and uniformly stirring, adding a pyrroloquinoline quinone solution (each kilogram of pyrroloquinoline quinone-containing solution contains 0 g, 0.6 g, 0.8 g, 1 g, 1.2 g and 1.4 g) according to a weight ratio of 1: 1, fully and uniformly stirring, and adding water until the finger seams of the hand holding material have water marks without dripping. Packaging the above materials in 200 bags according to the same specification, sterilizing and culturing according to the same method, collecting Auricularia, and calculating Auricularia dry weight and protein content.
Note: the sample is the content of pyrroloquinoline quinone (g/kg)
Compared with the control, when the content of pyrroloquinoline quinone is 0.8-1.2 g/kg, the growth speed of hyphae is accelerated, and the kilogram number of dry agaric produced by 100 kg of dry materials and the protein content are obviously increased. When the content of the pyrroloquinoline quinone is 1.0 g/kg, the yield is increased by (9.5-8.3)/8.3 by 14.5 percent compared with the control; the protein content increased%.
Claims (3)
1. An agaric bagged culture material additive and a using method thereof are characterized by comprising the following steps:
(1) adding a certain amount of pyrroloquinoline quinone into water, and fully dissolving to prepare pyrroloquinoline quinone solution.
(2) And fully and uniformly mixing the prepared bagged agaric culture material dry material and the pyrroloquinoline quinone solution according to a certain proportion, and continuously adding water to meet the humidity requirement of the culture material.
2. The agaric bagged culture material additive and the using method thereof as claimed in claim 1, wherein in the step (1), a certain amount of pyrroloquinoline quinone is added into water in a weight ratio of 0.8-1.2 (pyrroloquinoline quinone) to 1000 (water).
3. The agaric bagged culture material additive and the use method thereof as claimed in claim 1, wherein in the step (2), the prepared agaric bagged culture material dry material and the pyrroloquinoline quinone solution are fully and uniformly mixed according to the weight ratio of 1: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911277348.4A CN110915548A (en) | 2019-12-06 | 2019-12-06 | Auricularia auricula bagged culture material additive and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911277348.4A CN110915548A (en) | 2019-12-06 | 2019-12-06 | Auricularia auricula bagged culture material additive and using method thereof |
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|---|---|
| CN110915548A true CN110915548A (en) | 2020-03-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201911277348.4A Pending CN110915548A (en) | 2019-12-06 | 2019-12-06 | Auricularia auricula bagged culture material additive and using method thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047007A (en) * | 1990-04-23 | 1990-11-21 | 山东省青州市食用菌研究所 | The production method of Qingzhou Jew's ear |
| JPH06105677A (en) * | 1992-03-25 | 1994-04-19 | Nippon Paper Ind Co Ltd | Method for culturing mushroom hypha |
| JP2009201465A (en) * | 2008-02-29 | 2009-09-10 | Forestry & Forest Products Research Institute | Grifola froundosa yield-increasing agent, medium for grifola froundosa fruit-body cultivation using the same, medium or culture medium for proliferating grifola froundosa mycelium, and grifola froundosa cultivating method |
| CN101548623A (en) * | 2009-05-08 | 2009-10-07 | 伍宗文 | Cultivation method of black fungus |
| JP2012082165A (en) * | 2010-10-12 | 2012-04-26 | Chemo-Sero-Therapeutic Research Inst | New adjuvant |
| CN108575553A (en) * | 2018-04-11 | 2018-09-28 | 南安市创培电子科技有限公司 | A kind of black fungus implantation methods |
-
2019
- 2019-12-06 CN CN201911277348.4A patent/CN110915548A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047007A (en) * | 1990-04-23 | 1990-11-21 | 山东省青州市食用菌研究所 | The production method of Qingzhou Jew's ear |
| JPH06105677A (en) * | 1992-03-25 | 1994-04-19 | Nippon Paper Ind Co Ltd | Method for culturing mushroom hypha |
| JP2009201465A (en) * | 2008-02-29 | 2009-09-10 | Forestry & Forest Products Research Institute | Grifola froundosa yield-increasing agent, medium for grifola froundosa fruit-body cultivation using the same, medium or culture medium for proliferating grifola froundosa mycelium, and grifola froundosa cultivating method |
| CN101548623A (en) * | 2009-05-08 | 2009-10-07 | 伍宗文 | Cultivation method of black fungus |
| JP2012082165A (en) * | 2010-10-12 | 2012-04-26 | Chemo-Sero-Therapeutic Research Inst | New adjuvant |
| CN108575553A (en) * | 2018-04-11 | 2018-09-28 | 南安市创培电子科技有限公司 | A kind of black fungus implantation methods |
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Application publication date: 20200327 |