CN1047007A - The production method of Qingzhou Jew's ear - Google Patents

The production method of Qingzhou Jew's ear Download PDF

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Publication number
CN1047007A
CN1047007A CN 90105272 CN90105272A CN1047007A CN 1047007 A CN1047007 A CN 1047007A CN 90105272 CN90105272 CN 90105272 CN 90105272 A CN90105272 A CN 90105272A CN 1047007 A CN1047007 A CN 1047007A
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ear
qingzhou
jew
sack
auricularia auriculajudae
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CN1023679C (en
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张文阁
张冰华
张钧华
张颂华
徐洪昌
李国华
张冀华
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QINGZHOU CITY EDIBLE FUNGI INST SHANDONG PROV
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QINGZHOU CITY EDIBLE FUNGI INST SHANDONG PROV
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Abstract

The production method of Qingzhou Jew's ear is that the Qingzhou Jew's ear bacterial classification is introduced than cultivating in the strong basicity medium; In order to strengthen the medical functions of auricularia auriculajudae, in cultivating process,, make the Qingzhou Jew's ear of producing not only have strong stress resistance and the difficult advantage of polluting in process of production again through measures such as sprinkling herb liquids; And auricularia auriculajudae itself also has protein content height (more than 13%), the quality exquisiteness, and it is many and hyperlipidemia is had characteristics of outstanding significant curative effect to contain colloid.

Description

The production method of Qingzhou Jew's ear
The present invention is the production method of a kind of edible mushroom, the production method of the more definite Qingzhou Jew's ear of saying so.
Auricularia auriculajudae is a kind of of edible mushroom, and it is a traditional food, the use of can being used as medicine again.The tradition of auricularia auriculajudae is introduced edible fungus in slant acidity or the neutral medium for the material production method and is cultivated.The shortcoming that said method exists is can not grow in alkaline medium procreation, the native ear resistance difference of production and easy pollution of edible fungus.
Task of the present invention be to study successfully a kind of utilize the Qingzhou Jew's ear bacterial classification in than the strong basicity medium, cultivate and in cultivating process again by the method for spraying herb liquid produce rich in proteins (more than 13%), quality exquisiteness, strong stress resistance, to be difficult for polluting, contain colloid many and hyperlipidemia is had a Qingzhou Jew's ear of significant curative effect.
Particular content of the present invention:
One. the bacterial classification that the present invention uses: the Qingzhou Jew's ear bacterial strain of this institute acclimatization culture belongs to the Uricularia polytricha subspecies.
Two. the medium that the present invention uses:
1. medium is with material name and quality requirement thereof:
(1). wood chip: generally adopting hardwood sawdust, is optimum with the wood chip with poplar, birch, mulberry tree wherein: the wood chip quality requirement: do not have and go mouldy free from admixture.
(2). cotton seed hull: require nontoxic change, free from admixture.
(3). maize cob meal, the requirement nothing is gone mouldy, free from admixture.
(4). the wheat husband: the requirement nothing is gone mouldy, free from admixture.
(5). composite fertilizer: the composite fertilizer that requires nitrogenous, phosphorus, potassium.
(6). white lime: require free from admixture, water content is below 40%.
(7). corn flour: require free from admixture.
2. medium is with the formula rate of raw material:
(1). general formula rate:
Wood chip: 30-40%; Cotton seed hull: 51-33%; Maize cob meal: 8-10%; Corn flour: 5-8%;
Wheat husband: 5-8%; Composite fertilizer: 1% white lime: an amount of.
(2) optimum formula ratio:
Wood chip: 35%, cotton seed hull: 39%, maize cob meal: 10%; Corn flour: 8%, the wheat husband: 7%, composite fertilizer: 1%, white lime: an amount of.
Three, concrete production process of the present invention:
After bacterial classification and raw material are got all the ready by above-mentioned requirements, produce by following step again:
1. allotment medium:
Press earlier the culture medium prescription proportion requirement, weigh up medium is raw materials used, and then in material: the ratio of water=1: 13 adds entry (running water) and stirs, when stirring, add an amount of water content at the white lime 40% below, stir and add in the process of white lime and require to expect that temperature preferably is controlled at 15 ° of-30 ℃ of scopes adding water.The white lime addition will be decided on its material temperature height: 10 ℃ of following additions are 10%; Addition was 18% when addition was 15%, 25 °-30 ℃ when addition was 12%, 18 °-25 ℃ in the time of 11 °-15 ℃.The material that adds white lime must stir evenly and change down the step again over to.
2. pack, sterilization: medium is after adding white lime and stirring evenly, earlier (for example: polyethylene) or in the wide-mouth bottle with the plastic sack of its 14X33 centimetre of both ends open of packing into, the loading amount of each plastic sack is the 400-500 gram, then with plastic sack openings at two ends good seal, carry out normal pressure (100 ℃, 10-12 hour) or high pressure (vapour pressure is 1.5 gauge pressures, 3-3.5 hour) sterilization, material after the sterilization will drop to below 30 ℃, and storage is good standby.
3. inoculation, cultivation: also reducing to the two ends that room temperature (below 30 ℃) is equipped with the medium plastic sack after the sterilization, the mouth of good seal is opened access Qingzhou Jew's ear bacterial strain, bacterial strain access amount: it is 0.5-1.5 centimetre bacterium piece 2-3 that every end of plastic sack will be put into diameter, connect and planted again with the sack good seal, then plastic sack is crouched and put, and single one by one yard, carry out the pattern discharging, under 15-30 ℃, carry out lucifuge and cultivate.
4. carry out the equation of light, temperature difference management: when mycelia is covered with, when ripe, one deck shade be covered on the bacterium wall that the plastic sack sign indicating number rises, only allow the plastic sack two ends be subjected to light, budding from the plastic sack two ends in order to it like this.When original hase appears in the sack two ends, open the two ends sack, and add intense light irradiation (illuminance is the 300-2000 lux) and ventilate (will open the window every day and ventilate more than 3 times), ventilation also increases with the increase of auricle.(relative moisture is: 90-95%) also will to increase air humidity when adding intense light irradiation and ventilation.
5. spray liquid: " carrying out the equation of light, temperature difference management " in the stage, when auricle is grown up when diameter reaches 5 centimetres, along with the increase of ventilation also will be carried out spray concentration respectively and is equal to or less than the gibberellin aqueous solution that 0.5% the Ligusticum wallichii Chinese medicine aqueous solution and concentration are 10-20ppm.The concrete grammar of spray liquid is: spray above-mentioned soup with sprayer on auricle, every day is more than 3 times.Will note during spray liquid: what young ear spilt will lack, and what become that ear spills wants many, when temperature is lower than 15 ℃ or air humidity more than 95%, or does not want spray liquid the ventilation condition difference time.
6. the harvesting of auricularia auriculajudae: grow up when the edge undulation being arranged and have spore to launch when the auricle of auricularia auriculajudae, can pluck auricularia auriculajudae.After drying, auricularia auriculajudae under plucking is the finished product that the present invention produces.
7. recuperation of mycelia and recovery:
After auricularia auriculajudae is gathered, cut off the water 5-8 days, during cutting off the water, strengthen ventilation and illumination, and in canopy, spray one time, grow and spread to prevent insect pest and assorted bacterium with 5% limewash to the ear canopy.Pass through 5-8 days when the mycelia recuperation recovers, spray concentration is twice of the composite fertilizer of 0.5% nitrogenous, phosphorus, potassium on the sack of plastic sack two ends, for keeping the pH value of medium, after the water on the sack is done, the limewash supernatant of spray 5% on the sack of two ends is twice again, with the sack good seal, carry out the mycelia lucifuge and cultivate then, condition of culture is the same.After buddingging, as previously mentioned, carry out harvesting of the equation of light, temperature difference management, spray liquid, auricularia auriculajudae and recuperation and the recovery several steps of mycelia and produce Qingzhou Jew's ear.Medium in the plastic sack can only use 3-4 time when the duplication of production auricularia auriculajudae.
Advantage of the present invention and characteristics:
One. medium of the present invention is more alkaline medium.
Traditional auricularia auriculajudae cultural method is to use slant acidity or neutral medium, and the medium that the present invention uses is owing to added the white lime 5-18% that contains 40% following moisture content, so medium presents than strong basicity, the Qingzhou Jew's ear bacterial classification is introduced in this medium, made it grow the measured auricularia auriculajudae of matter by a series of control measures.
Two. the present invention sprays herb liquid in producing the Qingzhou Jew's ear process, strengthened the medical functions of Qingzhou Jew's ear:
Elaboration according to " each wood of auricularia auriculajudae is all living; its good poison also must be with wood ", the present invention except that in medium with the poplar bits, also in process of production, when the auricle diameter grows to 5 centimetres, spray the herb liquid number time that contains the Ligusticum wallichii composition every day, so just strengthened the function of the auricularia auriculajudae treatment hyperlipidemia that the present invention produces.
Three. Qingzhou Jew's ear strong stress resistance that the present invention produces and difficult the pollution:
Even stream ear phenomenon also often appears in common woodear in the good fluffy cellar for storing things of condition, go through evil sometimes even very, and just easily go mouldy when temperature rises to 28 ℃; But the Qingzhou Jew's ear that the present invention produces is all rare stream ear phenomenon in fluffy cellar for storing things or in the woods no matter, again because of Qingzhou Jew's ear is difficult for pollution, so also do not go mouldy even temperature rises to 36-37 ℃.
Four. Qingzhou Jew's ear protein content height and the more common auricularia auriculajudae exquisiteness of auricularia auriculajudae quality that the present invention produces, colloid is many especially:
National Specification auricularia auriculajudae protein content is more than 7.7%.Common auricularia auriculajudae protein content is after testing all in the 7-9% scope, and the Qingzhou Jew's ear protein content that the present invention produces is through Shandong University, its protein content detects all at (during auricularia auriculajudae water content 12%) more than 13% in the many families in Qingzhou City food quality inspection station and food quality inspection station, Shandong Province, surpasses more than 68% of national regulation minimum standard; Exceed 50% than other auricularia auriculajudae protein contents.The Qingzhou Jew's ear that the present invention produces is also fine and smooth and to contain colloid many because of the general auricularia auriculajudae of its quality, so stick-slip is good to eat in cooking.
Five. the Qingzhou Jew's ear that the present invention produces has significant curative effect to hyperlipidemia:
The high-grade nutrient food of still not a kind of rich in proteins of Qingzhou Jew's ear (more than 13%) that the present invention produces, and be a kind of herbal cuisine that hyperlipidemia is had significant curative effect, Qingzhou Jew's ear suffers from various degree by 140, and the hyperlipidemia patient takes, the effect that degree varies is all arranged only exemplifies the situation that seven bit string persons take Qingzhou Jew's ear below its curative effect is described: (one). test site: 402 hospitals of the Chinese People's Liberation Army
(2). test method:
1. Qingzhou Jew's ear consumption: every day 3 times, cooking is eaten, consumption per day 10-15 gram.
2.40 it is one-period, has a blood test the contrast of having a blood test again that is in 40 days before the test.
3. take during the Qingzhou Jew's ear all blood lipid-lowering medicines of stopping using.
(3). result of the test:
Figure 901052728_IMG1
Shown by the tabular result of the test: the effect for reducing blood fat of Qingzhou Jew's ear is apparent in view, and is especially more remarkable to high cholesterol patient effect.
Embodiment:
One. bacterial classification: Qingzhou Jew's ear bacterial strain
Two. medium: allocate medium: willow wood chip 35% by following formula rate, chicken girt core powder 10%, corn flour 5%, wheat husband 8%, composite fertilizer's (containing N.P.K) 1%, cotton seed hull 41%, totally 1200 kilograms of inventorys, in material: the ratio of water=1: 1.3 adds water and is mixed, and then to add water content in per hundred kilograms of siccatives be that the ratio of 15 kilograms of 35% white limes adds white lime and stirs evenly, behind the mixing material packed in the high pressure polyethylene plastic bag of 14X33 centimetre of both ends open, under 100 ± 1 ℃, the sterilization through 10 hours.When temperature is reduced to 30 ℃, the plastic sack two-port is opened after the sterilization, the Qingzhou Jew's ear bacterial strain is inserted in the medium.The inoculation method of bacterial strain and access amount: insert bacterial strain at the two ends of plastic sack, it is 0.5-1.5 centimetre bacterium piece 2-3 piece that each termination of bag is gone into diameter.After bacterial strain connects, the two ends sack is sealed again, cultivated under the lucifuge condition, cultivation temperature will be controlled at 15 ° of-30 ℃ of scopes, after auricle stretches air humidity is transferred to 90-95%, and illumination is controlled at the 300-2000 lux and windows every day ventilates more than 3 times; When the auricle diameter extended to 5 centimetres, temperature was more than 20 ± 1 ℃ the time, and spray 2-4 time respectively with the Ligusticum wallichii herb liquid of 0.5% concentration and the gibberellin soup of 20ppm every day, plucked to auricularia auriculajudae and adopted the eve and stop to spray liquid medicine again; Can pluck when having spore to distribute, the auricularia auriculajudae under plucking is through drying the finished product that is present embodiment.
The auricularia auriculajudae of present embodiment production detects through relevant department, and its protein content is 13.3%, and prove by 7 routine hyperlipidemia patients' the clinical result of taking through 402 hospitals of the Chinese People's Liberation Army: Qingzhou Jew's ear has obvious curative effects to hyperlipidemia; Especially the high-cholesterol disease patient more there is significant curative effect (test data is seen before, and face is routine to be shown).

Claims (10)

1, the production method of Qingzhou Jew's ear is characterized in that this method is that the Qingzhou Jew's ear bacterial classification is placed on than cultivating in the strong basicity medium, in cultivating process, allocate medium through [1], [2] pack, sterilization, [3] inoculation, cultivate, [4] carry out the equation of light, temperature difference management, [5] spray liquid, [6] harvesting of auricularia auriculajudae, [7] recuperation of mycelia is produced Qingzhou Jew's ear with the recovery several steps, with the Qingzhou Jew's ear of said method production except that the advantage that has strong stress resistance in process of production and be difficult for polluting, auricularia auriculajudae itself also has protein content up to more than 13%, the quality exquisiteness, and it is many and hyperlipidemia is had characteristics of significant curative effect to contain glue fat.
2, the production method according to the described Qingzhou Jew's ear of claim 1 is characterized in that the formula rate of the used medium of this method is:
Wood chip: 30-40%; Cotton seed hull: 51-33%; Maize cob meal: 8-10%;
Corn flour: 5-8%; Wheat husband: 5-8% contains N.P.K composite fertilizer: 1% white lime: an amount of
3,, it is characterized in that the used best prescription ratio of this method is according to the production method of the described Qingzhou Jew's ear of claim 1:
Wood chip: 35%; Cotton seed hull: 39%; Maize cob meal: 10%; Corn flour: 8%;
Wheat husband: 7%; Contain N.P.K composite fertilizer: 1% white lime: an amount of
4, it is characterized in that according to the described Qingzhou Jew's ear production method of claim 1 (1) allotment medium in this method is earlier by the culture medium prescription proportion requirement, weigh up medium is raw materials used, and then in material: the ratio of water=1: 13 adds running water and adds an amount of water content at the white lime below 40% when stirring.White lime addition: add 10%, 11 °-15 ℃ below 10 ℃ and add 15%, 25 °-30 ℃ addings 18% of 12%, 16 °-25 ℃ addings.When adding and expect, white lime changes down the step over to after stirring is spared.
5, it is characterized in that according to the described Qingzhou Jew's ear production method of claim 1 (2) in this method pack, sterilization, be meant that medium is after adding white lime and stirring evenly, earlier pack it into 14X33 centimetre, in the plastic sack or wide-mouth bottle of both ends open, each plastic sack is by the 400-500 gram device medium of packing into, then with plastic sack openings at two ends good seal, carry out under 100 ℃, 10-12 hour normal pressure pressed bacterium or carry out 3-3.5 hour autoclaving under the steam pressure of 1.5 gauge pressures, to expect after the sterilization below the temperature drop to 30 ℃
6, according to the described Qingzhou Jew's ear production method of claim 1 it is characterized in that in this method (3) inoculation, cultivate, be meant that after sterilization the two ends sack that the plastic sack of medium is housed is opened, insert the Qingzhou Jew's ear bacterial strain.The access amount of bacterial strain: will insert diameter in the end sack and be 0.5-1.5 centimetre bacterium piece 2-3 piece.Connect and planted, plastic sack has been crouched put and single one by one yard then, carried out the discharging of wall formula, under 15 °-30 ℃, carried out the lucifuge cultivation again with the sack good seal.
7, according to the production method of the described Qingzhou Jew's ear of claim 1, it is characterized in that (4) in this method carry out the equation of light, temperature difference management, be meant when mycelia and cover with, when ripe, on the bacterium wall that the plastic sack sign indicating number rises, cover one deck shade, only allow the plastic sack two ends be subjected to light.When original hase appears in the sack two ends, to open the two ends sack and add intense light irradiation and add forced ventilation, illuminance requires: the 300-2000 lux; Will open the window every day and ventilate more than 3 times and ventilation will increase with the increase of auricularia auriculajudae, also will increase air humidity when adding intense light irradiation and ventilation, relative moisture is controlled at 90-95%.
8, according to described its feature of Qingzhou Jew's ear production method of claim 1 (5) spray liquid in the method, be meant " carrying out the equation of light; temperature difference management " in the stage, when auricle is grown up when diameter reaches 5 centimetres along with the increase of ventilation, also to carry out the Ligusticum wallichii Chinese medicine aqueous solution of spray concentration≤0.5% and the gibberellin aqueous solution that concentration is 10-20ppm respectively, the concrete grammar of spray liquid is: with sprayer every day to spraying above-mentioned liquid medicine on the auricle respectively more than 3 times, will note during spray liquid: what young ear spilt will lack, and wanting that the one-tenth ear spills is many; When temperature is lower than 15 ℃ or relative air humidity more than 95% or when ventilating difference, spray liquid not.
9, it is characterized in that the harvesting of (6) auricularia auriculajudae in this method according to the production method of the described Qingzhou Jew's ear of claim 1, be meant when the auricle of auricularia auriculajudae and grow up when the edge undulation being arranged and have spore to launch, can pluck auricularia auriculajudae, be the finished product that the present invention produces after the auricularia auriculajudae under plucking is dried.
10, according to the production method of the described Qingzhou Jew's ear of claim 1 it is characterized in that in this method the recuperation of (7) mycelia with recover, be to cut off the water after auricularia auriculajudae is gathered 5-8 days, during cutting off the water, strengthen ventilation and illumination to the ear canopy, in extremely, spray one time with 5% limewash simultaneously, mycelia process 5-8 days rest and recovery, spray concentration 0.5%NPK composite fertilizer is twice on the plastic sack two-port, after the water on the sack is done, on the sack of two ends, spray 5% limewash twice again, then with sack good seal again, carry out the mycelia lucifuge and cultivate, condition of culture is the same.After buddingging, carry out the equation of light, temperature difference management as previously mentioned again, spray liquid, harvesting of auricularia auriculajudae and the recuperation of mycelia and recovery several steps are produced Qingzhou Jew's ear.Medium in the plastic sack can only be reused 3-4 time when producing auricularia auriculajudae.
CN 90105272 1990-04-23 1990-04-23 Production method for Qingzhou Jew's ear Expired - Fee Related CN1023679C (en)

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1047200C (en) * 1994-03-09 1999-12-08 刘永昶 Earth-cultivated black fungus and its cultivating method by use of substitute material for plastic bag
CN103214279A (en) * 2013-04-22 2013-07-24 邬金飞 Compatibility of black fungus cultivation material and manufacturing method of cultivation material
CN103460992A (en) * 2013-08-23 2013-12-25 福建省农业科学院农业工程技术研究所 Method for cultivating agaric by using pig feces bag
CN104311284A (en) * 2014-09-26 2015-01-28 侯中仁 Preparation method of black fungus culture medium
CN104909894A (en) * 2015-06-03 2015-09-16 广西大学 Black fungus culture medium and preparation method thereof
CN105165402A (en) * 2015-09-24 2015-12-23 龙里卓驰农业科技有限公司 Method for cultivating edible fungus in kitchen
CN105638239A (en) * 2015-12-31 2016-06-08 钦州市钦北区生产力促进中心 Efficient black fungus cultivation method
CN105936598A (en) * 2016-07-04 2016-09-14 贵州根生科技农业有限公司 Culture material for edible fungus and preparation method thereof
CN105961031A (en) * 2016-06-22 2016-09-28 潘雪玉 High-yield black fungus cultivation method
CN105961030A (en) * 2016-06-22 2016-09-28 潘雪玉 Black fungus cultivation method
CN106069190A (en) * 2016-06-22 2016-11-09 潘雪玉 The steam bacterium bag cultivation method of Auricularia
CN106069191A (en) * 2016-06-22 2016-11-09 潘雪玉 The bacterium bag cultivating method of Auricularia
CN106083230A (en) * 2016-06-22 2016-11-09 潘雪玉 Reduce the ecological cultivation method of content of beary metal in Auricularia
CN106069194A (en) * 2016-06-29 2016-11-09 金皓 A kind of method cultivating Ganoderma and collection Ganoderma spore powder
CN110915548A (en) * 2019-12-06 2020-03-27 安徽农业大学 Auricularia auricula bagged culture material additive and using method thereof

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1047200C (en) * 1994-03-09 1999-12-08 刘永昶 Earth-cultivated black fungus and its cultivating method by use of substitute material for plastic bag
CN103214279A (en) * 2013-04-22 2013-07-24 邬金飞 Compatibility of black fungus cultivation material and manufacturing method of cultivation material
CN103460992A (en) * 2013-08-23 2013-12-25 福建省农业科学院农业工程技术研究所 Method for cultivating agaric by using pig feces bag
CN104311284A (en) * 2014-09-26 2015-01-28 侯中仁 Preparation method of black fungus culture medium
CN104909894A (en) * 2015-06-03 2015-09-16 广西大学 Black fungus culture medium and preparation method thereof
CN105165402A (en) * 2015-09-24 2015-12-23 龙里卓驰农业科技有限公司 Method for cultivating edible fungus in kitchen
CN105638239A (en) * 2015-12-31 2016-06-08 钦州市钦北区生产力促进中心 Efficient black fungus cultivation method
CN105961031A (en) * 2016-06-22 2016-09-28 潘雪玉 High-yield black fungus cultivation method
CN105961030A (en) * 2016-06-22 2016-09-28 潘雪玉 Black fungus cultivation method
CN106069190A (en) * 2016-06-22 2016-11-09 潘雪玉 The steam bacterium bag cultivation method of Auricularia
CN106069191A (en) * 2016-06-22 2016-11-09 潘雪玉 The bacterium bag cultivating method of Auricularia
CN106083230A (en) * 2016-06-22 2016-11-09 潘雪玉 Reduce the ecological cultivation method of content of beary metal in Auricularia
CN106069194A (en) * 2016-06-29 2016-11-09 金皓 A kind of method cultivating Ganoderma and collection Ganoderma spore powder
CN106069194B (en) * 2016-06-29 2020-05-19 金皓 Method for cultivating lucid ganoderma and collecting lucid ganoderma spore powder
CN105936598A (en) * 2016-07-04 2016-09-14 贵州根生科技农业有限公司 Culture material for edible fungus and preparation method thereof
CN110915548A (en) * 2019-12-06 2020-03-27 安徽农业大学 Auricularia auricula bagged culture material additive and using method thereof

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