CN110896950B - Slow-release peroxyacetic acid disinfectant for bees and preparation method and application thereof - Google Patents
Slow-release peroxyacetic acid disinfectant for bees and preparation method and application thereof Download PDFInfo
- Publication number
- CN110896950B CN110896950B CN201911176808.4A CN201911176808A CN110896950B CN 110896950 B CN110896950 B CN 110896950B CN 201911176808 A CN201911176808 A CN 201911176808A CN 110896950 B CN110896950 B CN 110896950B
- Authority
- CN
- China
- Prior art keywords
- peroxyacetic acid
- retaining agent
- water
- disinfectant
- bees
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a slow-release peroxyacetic acid disinfectant for bees and a preparation method and application thereof, wherein the slow-release peroxyacetic acid disinfectant for bees comprises plant essential oil, a peroxyacetic acid solution and a high-molecular water-retaining agent, wherein the high-molecular water-retaining agent is a carrier of the plant essential oil and the peroxyacetic acid solution, and the weight ratio of the high-molecular water-retaining agent to the peroxyacetic acid solution is 1: 8-30 percent, and the addition amount of the plant essential oil is 0.1-5 percent by weight; the preparation method comprises weighing the raw materials according to formula ratio, mixing the raw materials except the macromolecular water-retaining agent uniformly, adding into the macromolecular water-retaining agent, stirring uniformly, and standing. The invention adopts the macromolecule water-retaining agent to be matched with each antibacterial component to form a colloidal mixture, and compared with the conventional liquid disinfectant, the operation safety is high, and the use is convenient; the sterilizing effect is good, the duration is long, the cost is low, the time and the labor are saved, and the bee is not toxic; the preparation method is simple and safe, low in price and easy to obtain, and has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of efficient and high-quality breeding of bees, relates to the technical field of prevention and control of bee diseases, and particularly relates to a slow-release peroxyacetic acid disinfectant for bees and a preparation method and application thereof.
Background
Honeybees are the most important pollinating insects in the world, play an irreplaceable role in maintaining plant diversity and ecological balance, and also greatly contribute to agricultural production and agricultural economic development.
In recent years, the growing colony collapse syndrome causes the number of colonies in some countries and regions to decrease continuously, and the development of the bee industry is greatly influenced. The number of bee populations is reduced due to a plurality of reasons, wherein various pathogenic microorganisms are always the problem of harming the healthy breeding and development of bees, for example, common bacterial bee diseases in the bee industry include American foul brood, European larva putrid disease, septicemia and paratyphoid disease; fungal bee diseases include chalkbrood disease and yellow aspergillosis; the bee diseases caused by viruses mainly include cysticercosis, paralysis agitans, bee pupa diseases and the like. Because bees have the characteristic of self-cleaning of the hive, many disease symptoms are not obvious and are detected before the disease occurs, so when the symptoms are obvious, the bee disease is developed seriously and is not easy to control, the health and development of bee colonies are affected, and even the bee colonies are collapsed. At present, no special mature measures are provided for preventing and treating bee diseases, most of the measures are used for applying medicines to diseases after the diseased bee swarms are found, but the effect is often unsatisfactory, and the loss of the bee swarms is caused.
In view of the difficulty in controlling bee disease, prevention remains the most important measure in bee production. The dry and clean beehive is kept, the beehive has clean beehive sites, beehive machines, hive spleens and the like, reduces the generation of pathogenic microorganisms and pests such as bacteria, mildew, viruses and the like in the beehive, effectively cuts off the propagation path, and can greatly improve the prevention effect. The traditional beehive and bee field disinfection mostly adopts formaldehyde fumigation or chlorine-containing disinfectant, has high toxicity and serious pollution and has great defects.
In conclusion, how to develop a novel disinfectant with high efficiency, safety and long-acting effect on the basis of the prior art is an urgent problem to be solved in the production of the bee industry.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a slow-release peroxyacetic acid disinfectant for bees and a preparation method and application thereof.
The invention adopts the following technical scheme:
the slow release type peroxyacetic acid disinfectant for bees comprises plant essential oil, a peroxyacetic acid solution and a high polymer water-retaining agent, wherein the high polymer water-retaining agent is a carrier of the plant essential oil and the peroxyacetic acid solution, and the weight ratio of the high polymer water-retaining agent to the peroxyacetic acid solution is 1: 8 to 30 percent, and the addition amount of the plant essential oil is 0.1 to 5 percent by weight.
Specifically, the peroxyacetic acid solution and the plant essential oil form a colloidal mixture after being absorbed by the macromolecular water-retaining agent, and the disinfection use time can reach more than 20 days.
In the technical scheme, the macromolecular water-retaining agent consists of acrylamide, acrylic acid and potassium hydroxide.
Specifically, the water-retaining agent consisting of acrylamide, acrylic acid and potassium hydroxide is a functional polymer material, can absorb hundreds of times and even thousands of times of water per se, has extremely strong water-retaining capacity, and can not escape when pressure is applied at normal temperature; the gel is not afraid of solar irradiation, can be retracted into small particles after being dried in the sun and absorb water again, can be used repeatedly, and has high gel degree, salt resistance, good stability and low cost; after the polymer water-retaining agent absorbs the peroxyacetic acid solution, the water can be locked, so that the risks of rapid reduction failure and liquid explosion of the peroxyacetic acid are avoided; no toxicity, no harm, no pollution, safe use and no secondary pollution.
Further, in the above technical scheme, the plant essential oil is Polegone, and the chemical component is 5-methyl-2-isopropylcyclohexanone.
Specifically, Polegone is the main component of the schizonepeta volatile oil, has an inhibition effect on acute inflammation of various animals, and has a better anti-epidemic virus effect; has pest repelling effect and insecticidal activity, and can effectively repel and kill Culex fatigues, Anopheles Fugu obscurus, Musca domestica and other mosquito and fly pests, and mite pests; polegone is one of the main components of the schizonepeta and mint volatile oil, can effectively inhibit the activity of staphylococcus aureus, escherichia coli, candida albicans, pseudomonas aeruginosa and the like, and reduces the morbidity.
Still further, in the above technical scheme, the slow release peroxyacetic acid disinfectant for bees further comprises a warning agent, and the amount of the warning agent added is 0.1-0.5 wt%.
Preferably, in the above technical solution, the warning agent is ferric oxide.
In a preferred embodiment, the slow-release peracetic acid disinfectant for bees comprises the following raw materials in percentage by weight:
the invention also provides a preparation method of the slow-release peroxyacetic acid disinfectant for bees, which comprises the following steps,
weighing the raw materials according to the formula ratio, uniformly mixing the raw materials except the macromolecular water-retaining agent, adding the mixture into the macromolecular water-retaining agent, uniformly stirring, and standing to obtain the water-retaining agent.
In the technical scheme, the peroxyacetic acid solution is an aqueous solution with the concentration of 0.1-10 wt%.
Preferably, in the above technical scheme, the concentration of the peroxyacetic acid solution is 2-10 wt%.
In the technical scheme, the particle diameter of the macromolecular water retaining agent is 30-400 meshes, preferably 60-100 meshes.
The invention also provides application of the slow-release peroxyacetic acid disinfectant for bees in bee breeding disinfection.
Specifically, the use method of the slow-release peroxyacetic acid disinfectant for bees is that bees do not need to be removed, and the slow-release disinfectant can be placed in a beehive after a cover of the beehive is opened during bee colony management, so that pathogenic bacteria in the beehive can be inhibited or killed, and the disinfectant is harmless to bees.
In detail, the slow-release type peroxyacetic acid disinfectant for bees can be placed in each corner of the beehive according to the volume of the beehive and the number of the bees, and can be used for more than 20 days.
Compared with the prior art, the invention has the following beneficial effects:
(1) the slow-release peroxyacetic acid disinfectant for bees provided by the invention adopts a colloidal mixture formed by matching a macromolecular water-retaining agent with peroxyacetic acid and the like, wherein the macromolecular water-retaining agent is composed of acrylamide, acrylic acid, potassium hydroxide and the like, is not afraid of solar irradiation, can be retracted into small particles after being dried in the sun and absorbs water again, can be repeatedly used, has high gelling degree and salt tolerance and good stability, and can lock water after absorbing peroxyacetic acid solution, so that the risks of fast reduction failure of peroxyacetic acid and liquid explosion are avoided;
(2) the slow-release peroxyacetic acid disinfectant for bees provided by the invention takes common peroxyacetic acid as a disinfecting component, has high safety and a wide sterilization spectrum, can effectively kill various germs by using with other active components, has a small using amount, can be continuously used for more than 20 days by fully adsorbing the high-molecular water-retaining agent and the peroxyacetic acid, has a long duration, has killing and repelling effects on various germs and bee mites by adding the natural plant extract Polegone, is safe for bees, has a synergistic effect by combining with the peroxyacetic acid, enhances the antibacterial effect of a formula, has low use cost, is convenient to use, saves time and labor, and is nontoxic to bees and larvae;
(3) the preparation method of the slow-release peroxyacetic acid disinfectant for bees provided by the invention has the characteristics of simple and safe production process, cheap and easily-obtained raw materials, good disinfection effect of the obtained product, great economic and social benefits and wide application prospect.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to specific examples.
The following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.
The experimental procedures used in the following examples are conventional unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The preparation method of the slow-release peroxyacetic acid disinfectant for bees in the embodiment and the comparative example of the invention is as follows:
step one, preparation of saturated peroxyacetic acid solution: fully and uniformly mixing commercial peroxyacetic acid A liquid and commercial peroxyacetic acid B liquid in a fume hood, and standing overnight to obtain a 15% saturated peroxyacetic acid solution;
step two, preparation of peroxyacetic acid solutions with different concentrations: in order to meet the requirements of subsequent tests, 15% saturated peroxyacetic acid solution is diluted into 2%, 5% and 10% peroxyacetic acid aqueous solution in sequence by deionized water.
And thirdly, weighing the raw materials according to the formula, uniformly mixing the raw materials except the macromolecular water-retaining agent, adding the mixture into the macromolecular water-retaining agent, uniformly stirring, and standing to obtain the water-retaining agent.
The high molecular water retention agent used in the examples and comparative examples of the invention is a commercially available high molecular water retention resin which specifically comprises acrylamide, acrylic acid and potassium hydroxide, and the fluff fiber is soft and has high bulk and forms a polymer similar to jelly after absorbing water.
Example 1A Slow Release Peracetic acid disinfectant for bees
1. Components
Wherein the particle size of the macromolecule water-retaining agent is over 120-180 meshes.
2. Preparation method
Weighing the raw materials according to the formula ratio, uniformly mixing Polegone, ferric oxide and 2 wt% of peroxyacetic acid solution, adding the mixture into a high-molecular water-retaining agent, uniformly stirring, and standing to obtain the water-retaining agent.
Example 2A Slow Release Peracetic acid disinfectant for bees
1. Components
Wherein the particle size of the macromolecular water-retaining agent is 60-100 meshes.
2. Preparation method
Weighing the raw materials according to the formula ratio, uniformly mixing Polegone, ferric oxide and 10 wt% of peroxyacetic acid solution, adding the mixture into a high-molecular water-retaining agent, uniformly stirring, and standing to obtain the water-retaining agent.
Example 3A Slow Release Peracetic acid disinfectant for bees
1. Components
Wherein the particle size of the macromolecular water-retaining agent is 60-100 meshes.
2. Preparation method
Weighing the raw materials according to the formula ratio, uniformly mixing Polegone, ferric oxide and 5 wt% of peroxyacetic acid solution, adding the mixture into a high-molecular water-retaining agent, uniformly stirring, and standing to obtain the water-retaining agent.
Example 4A Slow Release Peracetic acid disinfectant for bees
1. Components
Wherein the particle size of the macromolecular water-retaining agent is 30-60 meshes.
2. Preparation method
Weighing the raw materials according to the formula ratio, uniformly mixing Polegone, ferric oxide and 5 wt% of peroxyacetic acid solution, adding the mixture into a high-molecular water-retaining agent, uniformly stirring, and standing to obtain the water-retaining agent.
Comparative example 1 sustained-release peroxyacetic acid disinfectant for bees
1. Components
1 part by weight of macromolecular water-retaining agent
30 parts by weight of a 5% strength by weight peracetic acid solution
0.05 part by weight of ferric oxide;
wherein the particle size of the macromolecular water-retaining agent is 60-100 meshes.
2. Preparation method
Weighing the raw materials according to the formula ratio, uniformly mixing ferric oxide and 5 wt% of peroxyacetic acid solution, adding the mixture into a high-molecular water-retaining agent, uniformly stirring, and standing to obtain the product.
Result verification
1. Comparison of the gel state of the product with the duration of the Disinfection
1.1 the adhesive state of the products of the different formulation examples is shown in tables 1 and 2.
TABLE 1 adsorption Performance test of polymer water-retaining agents with different particle diameters on saturated peroxyacetic acid with different volume percentages of 15%
TABLE 2 adsorption Performance test of polymer water-retaining agents with different particle diameters on 5% peroxyacetic acid solutions with different volumes
The results in tables 1 and 2 are compared and analyzed, and it is found that the higher the concentration of the peracetic acid solution is, the poorer the adsorption performance of the polymer water retaining agent is.
Specifically, as can be seen from table 1, for 15 wt% saturated peracetic acid solution, the adsorption capacity of 200-400 mesh high molecular water retaining agent is the worst, and under the same formulation, 0.5g of 200-400 mesh high molecular water retaining agent can only adsorb <10mL of liquid; 0.5g of 0-60 mesh high molecular water-retaining agent has relatively good gel state, but when the volume of the solution is more than 10mL, the required adsorption time is prolonged, and the formed gel is loose and fragile; the 120-mesh 180-mesh and 60-100-mesh high molecular water retention agent has the best adsorption performance and the formed gel has good aggregation force, and can be used for screening the next experimental formula.
Further, after selecting a 5 wt% peroxyacetic acid solution and screening in table 1, further adsorption performance tests are carried out on the four polymer resins, and the adsorption capacity of the 200-mesh 400-mesh polymer water-retaining agent on the 5% peroxyacetic acid solution is less than 10 mL; when the total solution volume is 15mL, the 30-180 mesh high molecular water-retaining agent has good adsorption performance and high adsorption speed, and the formed gel has good adhesion, but the total adsorption volume of the 120-180 mesh high molecular water-retaining agent is probably smaller than that of the 30-60 mesh and 60-100 mesh high molecular water-retaining agents, and in view of the concentration gradient of the solution in the subsequent antivirus effect formula screening, the 30-60 mesh, 60-100 mesh and 120-180 mesh high molecular water-retaining agents are reserved for the subsequent embodiment verification.
1.2 duration of Disinfection of the products of the different formulation examples
50g of slow-release disinfectant is prepared according to the formula obtained in 1.1, and the slow-release disinfectant is placed in a beehive and periodically checked and counted for the release effect of the disinfectant, wherein the specific results are shown in the following table 3.
TABLE 3 Slow Release Performance test of Polymer Water absorbent with different particle diameters-Peroxyacetic acid solutions with different concentrations
The results in table 3 show that the volatile mass in the beehive with the polymer water-retaining agent is 30-60 meshes >60-100 meshes >120 meshes > 180 meshes, and the volatile mass in the concentration of peracetic acid with different concentrations is 10 wt% <5 wt% <2 wt%, i.e., the larger the water content is, the better the water-retaining effect of the polymer water-retaining agent is, the slower the volatilization is, and the longer the disinfection maintenance time is. According to the actual use time requirement in the beehive, 60-100 meshes of high molecular water-retaining agent is used as the water-retaining agent with the optimal particle size, and 5 wt% of peroxyacetic acid solution is used as the optimal disinfection concentration.
2. Comparison and verification of disinfection effect
2.1 according to the formula obtained in 1.1, respectively preparing a slow release disinfectant with the weight of 50g, transferring the slow release disinfectant into a slow release box, and then placing the slow release box into a previously prepared beehive;
specifically, after each slow-release disinfectant is placed in a beehive, the top of the beehive and the position farthest away from the disinfectant are wiped lightly by using a filter paper strip at regular time; further, igniting an alcohol lamp, putting the wiped filter paper strip into an LB culture medium within the range of 10cm around the alcohol lamp, culturing in an incubator at 37 ℃, and observing the growth condition of the bacterial colony after 24 hours; and the bactericidal effect of each disinfectant was evaluated at 3d, 6d, 9d and 14d after the slow-release disinfectant was placed in the hive, and the results are shown in table 4.
TABLE 4 variation of colony growth area of polymer water absorbent with different particle diameters and peracetic acid solutions with different concentrations
The results in Table 4 are analyzed, and it can be seen that, compared with the control group, the colony at the bottom of the beehive is relatively serious, the growth area on the plate culture medium is large, after different time treatment, the disinfectant has obvious disinfection effect on the upper part and the bottom of the beehive, and the sterilization effect of the 30-60 mesh high molecular water-retaining agent and the 60-100 mesh high molecular water-retaining agent is superior to that of the 120-mesh 180-mesh high molecular water-retaining agent and is consistent with the slow release performance; the disinfection effect of the slow release agent is in direct proportion to the concentration of the peroxyacetic acid, and the larger the concentration of the peroxyacetic acid is, the better the disinfection effect is.
2.2 inhibition of common germs by disinfectants
According to the mixture ratio of the above examples 1-4 and the comparative example 1, the in vitro bacteriostatic effect of the disinfectant is compared with that of the single components of 5 wt% peroxyacetic acid solution and 5 wt% Polegone, 100 μ L of the prepared disinfectant solution is added into the prepared culture solution of the disease bacteria by taking a blank of distilled water as a reference, the mixture is shaken up and cultured in an incubator, and the bacteriostatic effect is shown in the following table 5.
TABLE 5 comparison of the inhibitory effect of different disinfectants on common germs
Note: "+" indicates that the culture solution is turbid and has bacteria growing; "-" indicates clear culture and sterile growth.
The results in the table 5 are analyzed, so that the bacteriostatic effect of the peroxyacetic acid solution and the Polegone compound disinfectant is obviously better than the single bacteriostatic effect of the peroxyacetic acid solution and the Polegone compound disinfectant, the safety, the biological property and the performance of the macromolecular water-retaining agent of bees are comprehensively considered, and the effect of the slow-release disinfectant in the embodiment 3 is optimal; polegone has insufficient bacteriostatic performance, but after being compounded with peroxyacetic acid, the compound can obviously improve the bacteriostatic performance, has certain avoidance to other diseases of bee colonies, such as bee mites, and is helpful to the overall disease resistance of the bee colonies.
3. Bee safety test
And the safety of the disinfectant is synchronously counted and analyzed while the sterilizing effect of the disinfectant is verified.
Selecting experimental swarms with similar health conditions, and adjusting the swarms to ensure that each swamp has the same number of combs and similar number of bees, and simultaneously contains similar number of bees, larvae and the like, and the health conditions of queen bees are similar; the colonies were grouped and the safety of the slow release disinfectants of examples 1-4 was counted separately.
During the placing process of the disinfectant, no abnormal phenomena such as dead bees, escaping bees, irritability, lowering of queen bee oviposition and the like are observed; meanwhile, after the maximum efficacy period of the disinfectant, the disinfectant is removed, the disinfectant is continuously observed for 30 days (1 generation of bees), and abnormal phenomena such as dead bees, escaping bees, irritability, spawning reduction of queen bees and the like are also not observed. The above results show that the slow-release disinfectant provided by the embodiment of the invention has no potential harm to the development of bee colonies.
Finally, the above is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. The slow-release peroxyacetic acid disinfectant for bees is characterized by comprising the following raw materials in percentage by weight:
the polymer water-retaining agent is composed of acrylamide, acrylic acid and potassium hydroxide, the particle size is 60-100 meshes, the polymer water-retaining agent is a carrier of plant essential oil and peroxyacetic acid solution, and the weight ratio of the polymer water-retaining agent to the peroxyacetic acid solution is 1: 8-30, wherein the concentration of the peroxyacetic acid solution is 5 wt%, and the plant essential oil is 5-methyl-2-isopropylcyclohexanone.
2. A preparation method of the slow-release peroxyacetic acid disinfectant for bees, which is characterized by comprising the steps of weighing the raw materials according to the formula, uniformly mixing the raw materials except for the macromolecular water-retaining agent, adding the mixture into the macromolecular water-retaining agent, uniformly stirring, and standing to obtain the disinfectant.
3. The use of the slow release peroxyacetic acid disinfectant for bees of claim 1 for disinfecting the bee breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911176808.4A CN110896950B (en) | 2019-11-26 | 2019-11-26 | Slow-release peroxyacetic acid disinfectant for bees and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911176808.4A CN110896950B (en) | 2019-11-26 | 2019-11-26 | Slow-release peroxyacetic acid disinfectant for bees and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110896950A CN110896950A (en) | 2020-03-24 |
| CN110896950B true CN110896950B (en) | 2021-12-07 |
Family
ID=69819756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911176808.4A Active CN110896950B (en) | 2019-11-26 | 2019-11-26 | Slow-release peroxyacetic acid disinfectant for bees and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110896950B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115226730A (en) * | 2022-08-24 | 2022-10-25 | 合肥中龙神力动物药业有限公司 | Volatile disinfectant for animal house and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1227465A (en) * | 1996-06-13 | 1999-09-01 | 维塔(欧洲)有限公司 | Method for control of infestations of honeybee colonies |
| CN106044894A (en) * | 2016-08-08 | 2016-10-26 | 余志娟 | Spinning sewage environmental-protection treating agent |
-
2019
- 2019-11-26 CN CN201911176808.4A patent/CN110896950B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1227465A (en) * | 1996-06-13 | 1999-09-01 | 维塔(欧洲)有限公司 | Method for control of infestations of honeybee colonies |
| CN106044894A (en) * | 2016-08-08 | 2016-10-26 | 余志娟 | Spinning sewage environmental-protection treating agent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110896950A (en) | 2020-03-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110732014B (en) | Traditional Chinese medicine composition for removing mites as well as preparation method and application thereof | |
| KR20160079371A (en) | Wild animal Repellent Composition and preparation thereof | |
| CN103783030B (en) | The method of the green ground beetle of a kind of predatory natural enemy insect and green muscardine fungus combination control insect | |
| CN110896950B (en) | Slow-release peroxyacetic acid disinfectant for bees and preparation method and application thereof | |
| CN106234388B (en) | A kind of composition pesticide of alkene containing benzo fluorine bacterium azoles and jamaicin | |
| CN107197861A (en) | A kind of CPV and the insecticidal suspending agent of muscardine and preparation method thereof | |
| CN110679612A (en) | Agricultural insecticidal composition for preventing and treating corn borers | |
| CN1628520A (en) | Externally used preparation for household pet disinsection and sterilization | |
| CN116210728A (en) | Bactericide and preparation method and application thereof | |
| CN113973825B (en) | Application of allicin in preparation of insect virus synergist and synergist | |
| KR101400713B1 (en) | Composition of pesticide containing pesticidal essential oils and/or food additives | |
| CN109221108A (en) | A kind of stifling composition and its application in prevention and treatment straw mushroom pest | |
| CN111096338B (en) | Attractant for tea seed elephant | |
| CN108308201A (en) | Red bayberry special-purpose insecticide | |
| CN112586508A (en) | Disinfectant for silkworm pathogenic microorganisms and application thereof | |
| CN107087639B (en) | Chitin-containing virus insecticidal suspending agent and preparation method thereof | |
| CN111011401A (en) | Mosquito killing medicament and preparation process thereof | |
| CN107184628A (en) | A kind of antibacterial film forming spray for animals | |
| CN116019116B (en) | Pesticide powder and application method thereof | |
| CN108013058B (en) | Disinfectant for silkworms and application thereof | |
| CN111714502B (en) | Application of solasodine in preparing medicine for killing fish ectoparasite | |
| SU1822795A1 (en) | Method for decontamination of air in poultry houses | |
| KR102603898B1 (en) | Agents for removing egg of poultry red mite | |
| KR102106921B1 (en) | The composition of livestock disinfectant which improved pest repellent effect | |
| CN108935337B (en) | Technology for raising breeding success rate and breeding number of scleroderma guani |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |