CN108013058B - Disinfectant for silkworms and application thereof - Google Patents
Disinfectant for silkworms and application thereof Download PDFInfo
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- CN108013058B CN108013058B CN201810042864.8A CN201810042864A CN108013058B CN 108013058 B CN108013058 B CN 108013058B CN 201810042864 A CN201810042864 A CN 201810042864A CN 108013058 B CN108013058 B CN 108013058B
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- disinfectant
- silkworms
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- fludioxonil
- silkworm
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 100
- 239000000645 desinfectant Substances 0.000 title claims abstract description 50
- MUJOIMFVNIBMKC-UHFFFAOYSA-N fludioxonil Chemical compound C=12OC(F)(F)OC2=CC=CC=1C1=CNC=C1C#N MUJOIMFVNIBMKC-UHFFFAOYSA-N 0.000 claims abstract description 49
- 239000005781 Fludioxonil Substances 0.000 claims abstract description 48
- MKIMSXGUTQTKJU-UHFFFAOYSA-N Propamocarb hydrochloride Chemical compound [Cl-].CCCOC(=O)NCCC[NH+](C)C MKIMSXGUTQTKJU-UHFFFAOYSA-N 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 230000000384 rearing effect Effects 0.000 claims abstract description 22
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 238000005507 spraying Methods 0.000 claims description 4
- 230000002147 killing effect Effects 0.000 abstract description 11
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- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 231100000378 teratogenic Toxicity 0.000 abstract description 3
- 230000003390 teratogenic effect Effects 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 29
- 230000004083 survival effect Effects 0.000 description 24
- 241000751139 Beauveria bassiana Species 0.000 description 22
- 230000001580 bacterial effect Effects 0.000 description 22
- 238000009395 breeding Methods 0.000 description 18
- 230000001488 breeding effect Effects 0.000 description 18
- 240000000249 Morus alba Species 0.000 description 16
- 235000008708 Morus alba Nutrition 0.000 description 16
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- 239000006013 carbendazim Substances 0.000 description 10
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- 238000007598 dipping method Methods 0.000 description 9
- 239000003899 bactericide agent Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
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- 230000000844 anti-bacterial effect Effects 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 230000000249 desinfective effect Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000008399 tap water Substances 0.000 description 5
- 235000020679 tap water Nutrition 0.000 description 5
- PFFIDZXUXFLSSR-UHFFFAOYSA-N 1-methyl-N-[2-(4-methylpentan-2-yl)-3-thienyl]-3-(trifluoromethyl)pyrazole-4-carboxamide Chemical compound S1C=CC(NC(=O)C=2C(=NN(C)C=2)C(F)(F)F)=C1C(C)CC(C)C PFFIDZXUXFLSSR-UHFFFAOYSA-N 0.000 description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 4
- 239000005760 Difenoconazole Substances 0.000 description 4
- 239000005808 Metalaxyl-M Substances 0.000 description 4
- 239000005816 Penthiopyrad Substances 0.000 description 4
- 239000005843 Thiram Substances 0.000 description 4
- 239000005845 Tolclofos-methyl Substances 0.000 description 4
- 150000001299 aldehydes Chemical class 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 4
- 229910052801 chlorine Inorganic materials 0.000 description 4
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
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- 239000000843 powder Substances 0.000 description 4
- MXMXHPPIGKYTAR-UHFFFAOYSA-N silthiofam Chemical compound CC=1SC([Si](C)(C)C)=C(C(=O)NCC=C)C=1C MXMXHPPIGKYTAR-UHFFFAOYSA-N 0.000 description 4
- WOSNCVAPUOFXEH-UHFFFAOYSA-N thifluzamide Chemical compound S1C(C)=NC(C(F)(F)F)=C1C(=O)NC1=C(Br)C=C(OC(F)(F)F)C=C1Br WOSNCVAPUOFXEH-UHFFFAOYSA-N 0.000 description 4
- KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 4
- 229960002447 thiram Drugs 0.000 description 4
- OBZIQQJJIKNWNO-UHFFFAOYSA-N tolclofos-methyl Chemical compound COP(=S)(OC)OC1=C(Cl)C=C(C)C=C1Cl OBZIQQJJIKNWNO-UHFFFAOYSA-N 0.000 description 4
- LQDARGUHUSPFNL-UHFFFAOYSA-N 1-[2-(2,4-dichlorophenyl)-3-(1,1,2,2-tetrafluoroethoxy)propyl]1,2,4-triazole Chemical compound C=1C=C(Cl)C=C(Cl)C=1C(COC(F)(F)C(F)F)CN1C=NC=N1 LQDARGUHUSPFNL-UHFFFAOYSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 239000005840 Tetraconazole Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000252233 Cyprinus carpio Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- CEJLBZWIKQJOAT-UHFFFAOYSA-N dichloroisocyanuric acid Chemical compound ClN1C(=O)NC(=O)N(Cl)C1=O CEJLBZWIKQJOAT-UHFFFAOYSA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- YKRQBWKLHCEKQH-KHPPLWFESA-N ethyl (z)-3-amino-2-cyano-3-phenylprop-2-enoate Chemical compound CCOC(=O)C(\C#N)=C(/N)C1=CC=CC=C1 YKRQBWKLHCEKQH-KHPPLWFESA-N 0.000 description 2
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- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 description 1
- 239000005747 Chlorothalonil Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 240000004429 Tephrosia purpurea Species 0.000 description 1
- 235000017517 Tephrosia purpurea Nutrition 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- -1 cyazoxystrobin Chemical compound 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a disinfectant for silkworms and application thereof, belonging to the technical field of prevention and treatment of silkworm diseases. The disinfectant for silkworms comprises the following raw material components in parts by weight: 1-20 parts of fludioxonil, 1-15 parts of propamocarb hydrochloride and 10000 parts of water; mixing the above materials, and sterilizing silkworm rearing room or rearing bed. The disinfectant for the silkworms is prepared by mixing fludioxonil and propamocarb hydrochloride for the first time, has a synergistic promoting effect on killing white muscardine silkworm by combining the fludioxonil and the propamocarb hydrochloride, improves the killing effect, is safe and efficient, has no teratogenic, mutagenic and carcinogenic effects on animals, and belongs to an environment-friendly disinfectant safe to ecological environment.
Description
Technical Field
The invention relates to a disinfectant for silkworms and application thereof, belonging to the technical field of prevention and treatment of silkworm diseases.
Background
China is a world production base for high-quality raw material cocoons, and the export amount accounts for more than 80% of the world. The direct economic loss caused by silkworm diseases in the silkworm breeding production accounts for 10-15% of the total income, the silkworm white muscardine disease is one of main diseases in the silkworm cocoon production, and the occurrence of the diseases brings great loss to the silkworm cocoon production.
In the prior art, chlorine preparations such as bleaching powder, dichloroisocyanuric acid and the like and aldehyde preparations such as formaldehyde and the like are widely used. Chinese patent document CN101233853 (application number 200810020439.5) provides a compound disinfectant for silkworms for disinfecting silkworm rearing beds, which consists of sodium dichloroisocyanurate and chlorothalonil in a weight ratio of 90-60: 10-40. The use of chlorine preparations and aldehyde preparations has been banned in developed countries since the last two decades of research, which has shown that chlorine preparations and aldehyde preparations pose a great threat to the health of humans and animals, and cause great pollution and damage to the environment. Continued use of large quantities of chlorine and aldehyde preparations is highly detrimental to sustainable development.
Fludioxonil (fludioxonil), chemical name: 4- (2, 2-difluoro-1, 3-benzodioxol-4-yl) pyrrole-3-carbonitrile of the formula: c12H6F2N2O2The bactericide is a non-systemic pyrrole bactericide, finally causes the death of germs by inhibiting the transfer related to the phosphorylation of glucose and inhibiting the growth of fungal mycelia, has unique action mechanism and has no cross resistance with the existing bactericide. Fludioxonil has no irritation to rabbit eyes and skin, and fish poison (96h, mg/L) LC500.31 of large turnfish, 1.5 of carp and 0.5 of crouching fish, no toxicity to bees and LC (liquid chromatography) for water fleas50(48h)1.1 mg/L. The Ames and other test results show that the product is not teratogenic, mutagenic and carcinogenic, and is one of the products which are mostly certified by the environmental protection agency EPA with low risk in the world.
Propamocarb hydrochloride (propamocarb hydrochloride), chemical name: propyl N- (3- (dimethylamino) propyl) carbamate hydrochloride having the formula: c9H21ClN2O2The bactericidal agent is a systemic bactericide, and the bactericidal mechanism of the bactericidal agent is mainly to inhibit the biosynthesis of phospholipid and fatty acid of germ cell membrane components, thereby inhibiting the growth of hyphae and the formation and germination of sporangium. Pericarb hydrochloride has no irritation to eyes and skin of tested animal, and rat can take LD through acute oral administration508600mg/kg, is safe to fish and bee, and Cyprinus Carpio LC50(72h)320 mg/L. The propamocarb hydrochloride can be mineralized rapidly in water without polluting underground water, is photolyzed for 97 percent under the action of microorganisms within 3 days, and belongs to an environment-friendly bactericide with safe ecological environment.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a disinfectant for silkworms and application thereof.
The technical scheme of the invention is as follows:
the disinfectant for the silkworms comprises the following raw material components in parts by weight:
1-20 parts of fludioxonil, 1-15 parts of propamocarb hydrochloride and 10000 parts of water; mixing the above raw materials.
According to the invention, the disinfectant for silkworms preferably comprises the following raw material components in parts by weight: 4-7 parts of fludioxonil, 3-5 parts of propamocarb hydrochloride and 10000 parts of water.
According to a further preferred aspect of the present invention, in the disinfectant for silkworms, fludioxonil is in parts by weight: 4 parts, 5 parts, 6 parts or 7 parts.
According to a further preferred aspect of the present invention, in the disinfectant for silkworms, propamocarb hydrochloride comprises, in parts by weight: 3 parts, 4 parts or 5 parts.
The disinfectant for silkworm is applied to disinfection of a rearing instrument and a rearing bed in a rearing room.
In the application, the disinfection step of the rearing instrument in the rearing room is as follows:
the disinfectant for silkworms is added according to the volume of 200-300 mL/m2The spraying amount is sprayed on the silkworm rearing instrument in a silkworm rearing room or the silkworm rearing instrument is soaked in the disinfectant for 20-40 min.
In the application, the silkworm rearing bed disinfection step is as follows:
the disinfectant for silkworms is added according to the volume of 200-300 mL/m2The spraying amount is sprayed on the silkworm rearing bed.
The invention has the following beneficial effects:
1. the disinfectant for the silkworm is prepared by mixing fludioxonil and propamocarb hydrochloride for the first time, and the fludioxonil and the propamocarb hydrochloride are used in a combined manner to kill white muscardine silkworm, so that the synergistic promotion effect is achieved, and the killing effect is improved;
2. the disinfectant is safe and efficient, has no teratogenic, mutagenic and carcinogenic effects on animals, and belongs to an environment-friendly bactericide which is safe in ecological environment;
3. the product has simple application method and is beneficial to large-scale popularization and application.
Detailed Description
The invention is further illustrated with reference to the following examples, without limiting the scope of the invention.
The fludioxonil was purchased from the GnIto crop protection company, GnIto, and the propamocarb hydrochloride was purchased from Bayer crop science, Germany.
Example 1
The disinfectant for the silkworms comprises the following raw material components in parts by weight:
fludioxonil 0.5g, propamocarb hydrochloride 0.4g, water 1000 g; the fludioxonil and the propamocarb hydrochloride are dissolved in water and mixed evenly to obtain the fludioxonil propamocarb hydrochloride.
The following experiment was carried out on the disinfecting effect of the disinfectant for silkworms prepared in example 1:
the medicine group is as follows:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7piece/mL) 200 μ L of the disinfectant for silkworms prepared in example 1 is uniformly mixed with 200 μ L of the disinfectant for silkworms prepared in example 1, after 30 minutes, the mixture is centrifuged at 4000rpm for 5 minutes, upper liquid is sucked away to terminate disinfection, equal amount of water is added to resuspend thalli, the surface of the silkworm body is coated until the silkworm body is wet after 2-year-old silkworm body is coated, after the silkworm body is kept wet and bred for 24 hours by attaching water-dipping filter paper in a culture dish cover, normal mulberry leaf breeding is corrected, and the survival rate is investigated;
control group:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7Piece/ml) 200 mu L of the mulberry leaf powder and 200 mu L of tap water are uniformly mixed, after 30 minutes, the surface of a silkworm body which is 2 years old is coated until the silkworm body is wet, water dipping filter paper is attached to the inside of a culture dish cover for moisturizing and breeding for 24 hours, normal mulberry leaf breeding is corrected, and the survival rate is investigated after two years;
the results are shown in Table 1, calculated by the following calculation formula:
TABLE 1
Example 2
The disinfectant for the silkworms comprises the following raw material components in parts by weight:
fludioxonil 0.5g, propamocarb hydrochloride 0.3g, water 1000 g; the fludioxonil and the propamocarb hydrochloride are dissolved in water and mixed evenly to obtain the fludioxonil propamocarb hydrochloride.
The following experiment was carried out on the disinfecting effect of the disinfectant for silkworms prepared in example 2:
the medicine group is as follows:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7piece/mL) 200 μ L of the disinfectant for silkworms prepared in example 2 is uniformly mixed with 200 μ L of the disinfectant for silkworms prepared in example 2, after 25 minutes, the mixture is centrifuged at 4000rpm for 5 minutes, the upper liquid is sucked away to terminate disinfection, an equal amount of water is added to resuspend the thalli, the surfaces of the silkworms after 2 years old are coated until the silkworms are wet, after a culture dish cover is internally attached with water-dipped filter paper for moisturizing and breeding for 24 hours, the normal mulberry leaf breeding is corrected, and the survival rate is investigated after two years old;
control group:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7Piece/ml) 200 mu L of the mulberry leaf powder and 200 mu L of tap water are uniformly mixed, after 25 minutes, the surface of a silkworm body which is 2 years old is coated until the silkworm body is wet, water dipping filter paper is attached to the inside of a culture dish cover for moisturizing and breeding for 24 hours, normal mulberry leaf breeding is corrected, and the survival rate is investigated after two years;
the results are shown in Table 2, calculated by the following calculation formula:
TABLE 2
Example 3
The disinfectant for the silkworms comprises the following raw material components in parts by weight:
fludioxonil 0.7g, propamocarb hydrochloride 0.3g, water 1000 g; the fludioxonil and the propamocarb hydrochloride are dissolved in water and mixed evenly to obtain the fludioxonil propamocarb hydrochloride.
The following experiment was carried out on the disinfecting effect of the disinfectant for silkworms prepared in example 3:
the medicine group is as follows:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7piece/mL) 200. mu.L of the disinfectant for silkworms prepared in example 3 was mixed uniformly, centrifuged at 4000rpm for 5min after 35 minutes, the upper liquid was aspirated off to terminate the disinfection, an equal amount of water was added to resuspend the thalli, the surface of the silkworm after 2 years old was smeared until wet, and after incubation for 24 hours with water-soaked filter paper in the lid of the culture dish, the normal conditions were correctedFeeding mulberry leaves, and investigating survival rate after two ages;
control group:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7Piece/ml) 200 mu L of the mulberry leaf powder and 200 mu L of tap water are uniformly mixed, after 35 minutes, the surface of a silkworm body which is 2 years old is coated until the silkworm body is wet, water dipping filter paper is attached to the inside of a culture dish cover for moisturizing and breeding for 24 hours, normal mulberry leaf breeding is corrected, and the survival rate is investigated after two years;
the results are shown in Table 3, calculated by the following calculation formula:
TABLE 3
Example 4
The disinfectant for the silkworms comprises the following raw material components in parts by weight:
fludioxonil 0.3g, propamocarb hydrochloride 0.2g, water 1000 g; the fludioxonil and the propamocarb hydrochloride are dissolved in water and mixed evenly to obtain the fludioxonil propamocarb hydrochloride.
The following experiment was carried out on the disinfecting effect of the disinfectant for silkworms prepared in example 4:
the medicine group is as follows:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7piece/mL) 200 μ L of the disinfectant for silkworms prepared in example 4 was uniformly mixed with 200 μ L of the disinfectant for silkworms, centrifuged at 4000rpm for 5min after 35 minutes, the upper liquid was aspirated off to terminate the disinfection, an equal amount of water was added to resuspend the thalli, the surface of the silkworm body from 2 years old was coated until moist, after incubation for 24 hours with water-soaked filter paper in the lid of the culture dish, normal mulberry leaf feeding was corrected, and the survival rate was investigated after two years;
control group:
the beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7Piece/ml) 200 mu L of the mulberry leaf powder and 200 mu L of tap water are uniformly mixed, after 35 minutes, the surface of a silkworm body which is 2 years old is coated until the silkworm body is wet, water dipping filter paper is attached to the inside of a culture dish cover for moisturizing and breeding for 24 hours, normal mulberry leaf breeding is corrected, and the survival rate is investigated after two years;
the results are shown in Table 4, calculated by the following calculation formula:
TABLE 4
Example 5
Pericarb hydrochloride test for promoting fludioxonil to kill beauveria bassiana
Application group:
experimental group 1: preparing a solution containing fludioxonil with the mass concentration of 0.04%;
experimental group 2: preparing a solution containing fludioxonil with the mass concentration of 0.07%;
experimental group 3: preparing a solution containing propamocarb hydrochloride with the mass concentration of 0.03%;
experimental group 4: preparing a solution containing propamocarb hydrochloride with the mass concentration of 0.05%;
experimental group 5: preparing a solution containing fludioxonil with the mass concentration of 0.04% and propamocarb hydrochloride with the mass concentration of 0.03%;
experimental group 6: a solution containing fludioxonil and propamocarb hydrochloride in a mass concentration of 0.07% was prepared.
Experimental methods
The beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7Pieces/ml) 200. mu.L of the disinfectant is uniformly mixed with 200. mu.L of the disinfectant for silkworms prepared in each experimental group of example 5, after 25 minutes, the mixture is centrifuged at 4000rpm for 5 minutes, upper liquid is sucked away to stop disinfection, equal amount of water is added to resuspend thalli, the surface of a silkworm body is coated to be moist after 2 years, after a culture dish cover is attached with water-dipping filter paper for moisture preservation and breeding is carried out for 24 hours, the normal mulberry leaf breeding is corrected, the survival rate is investigated after two years and calculated by the following calculation formula:
the calculation results are shown in table 5;
TABLE 5 propamocarb hydrochloride promotion of fludioxonil to Beauveria bassiana killing test
| Group of | Survival rate of silkworm% |
| Experimental group 1 | 80.5 |
| Experimental group 2 | 86.3 |
| Experimental group 3 | 0 |
| Experimental group 4 | 0 |
| Experimental group 5 | 97.1 |
| Experimental group 6 | 98.3 |
As can be seen from the data in Table 5, fludioxonil has a certain killing effect on Beauveria bassiana of Bombyx mori, but the effect is not ideal. The propamocarb hydrochloride and the fludioxonil are compounded according to a certain proportion, and the compounded medicines have a good synergistic effect, so that the killing effect on the white muscardine silkworm is improved.
Comparative example 1
Difenoconazole was purchased from pilot plant for pesticides in the concourse of the plant protection institute of the academy of agricultural sciences, tetraconazole was purchased from Italy seiger, and tolclofos-methyl was purchased from eastern gold chemical Co., Ltd.
Application group:
experimental group 1: preparing a solution containing 0.05% of difenoconazole by mass concentration;
experimental group 2: preparing a solution containing 0.05% of tetraconazole by mass concentration;
experimental group 3: preparing a solution containing 0.05% of tolclofos-methyl.
Experimental methods
200 mu L of the solution prepared in each experimental group of the comparative example 1 is respectively and uniformly mixed with 200 mu L of tap water, the surface of the silkworm body after 2 years old is coated until the silkworm body is wet, water dipping filter paper is attached to the inside of a culture dish cover for moisturizing and raising for 24 hours, the normal mulberry leaf raising is corrected, the poisoning condition of the silkworm is investigated after one year, and the calculation is carried out by the following calculation formula:
the calculation results are shown in table 6;
TABLE 6 influence of Difenoconazole, Tetraflutriazole and tolclofos-methyl on the survival rate of Bombyx mori
| Group of | Survival rate of silkworm% |
| Experimental group 1 | 36.9 |
| Experimental group 2 | 22.4 |
| Experimental group 3 | 10.8 |
As can be seen from the data of table 6, difenoconazole, tetraconazole and tolclofos-methyl can cause poisoning of silkworms.
Comparative example 2
metalaxyl-M is purchased from Zhejiang Yifan chemical company, Cyanoxastrobin is purchased from Jiangsu province pesticide research institute company, thifluzamide is purchased from Hebei Guanlong agricultural chemical company, silthiopham is purchased from Monsanto company, penthiopyrad is purchased from Guangdong Wengjiang chemical reagent company, and thiram is purchased from Hebei Zanfeng bioengineering company.
Application group:
experimental group 1: preparing a solution containing metalaxyl-M with the mass concentration of 0.1%;
experimental group 2: preparing a solution containing the phenamacril with the mass concentration of 0.1 percent;
experimental group 3: preparing a solution containing 0.1% of thifluzamide by mass concentration;
experimental group 4: preparing a solution containing silthiopham with the mass concentration of 0.1%;
experimental group 5: preparing a solution containing penthiopyrad with the mass concentration of 0.1%;
experimental group 6: preparing a solution containing 0.1% thiram by mass concentration;
experimental group 7: preparing a solution containing fludioxonil with the mass concentration of 0.05%;
experimental group 8: preparing a solution containing 0.05 percent of metalaxyl-M and 0.05 percent of fludioxonil in mass concentration;
experimental group 9: preparing a solution containing the phenamacril with the mass concentration of 0.05 percent and the fludioxonil with the mass concentration of 0.05 percent;
experimental group 10: preparing a solution containing 0.05% of thifluzamide and 0.05% of fludioxonil in mass concentration;
experimental group 11: preparing a solution containing silthiopham with the mass concentration of 0.05% and fludioxonil with the mass concentration of 0.05%;
experimental group 12: preparing a solution containing penthiopyrad with the mass concentration of 0.05% and fludioxonil with the mass concentration of 0.05%;
experimental group 13: a solution containing thiram at a mass concentration of 0.05% and fludioxonil at a mass concentration of 0.05% was prepared.
Experimental methods
The beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7Piece/ml) 200 mul are respectively and uniformly mixed with 200 mul of solution prepared by each experimental group of the comparative example 2, after 25 minutes, the mixture is centrifuged at 4000rpm for 5 minutes, upper liquid is sucked away to stop disinfection, equal amount of water is added to resuspend thalli, the surfaces of the silkworm bodies after 2 years are coated until the silkworm bodies are wet, after a culture dish cover is internally attached with water-dipping filter paper for moisturizing and breeding for 24 hours, the normal mulberry leaf breeding is corrected, the survival rate is investigated after two years, and the survival rate is calculated by the following calculation formula:
the calculation results are shown in table 7;
influence of surface 76 disinfectants and disinfectants combined with fludioxonil on silkworm survival rate
| Group of | Survival rate of silkworm% |
| Experimental group 1 | 0 |
| Experimental group 2 | 0 |
| Experimental group 3 | 0 |
| Experimental group 4 | 0 |
| Experimental group 5 | 0 |
| Experimental group 6 | 0 |
| Experimental group 7 | 82.2 |
| Experimental group 8 | 81.2 |
| Experimental group 9 | 80.6 |
| Experimental group 10 | 82.4 |
| Experimental group 11 | 81.8 |
| Experimental group 12 | 83.5 |
| Experimental group 13 | 83.2 |
As can be seen from the data in Table 7, metalaxyl-M, cyazoxystrobin, thifluzamide, silthiopham, penthiopyrad and thiram have no killing effect on Beauveria bassiana and have no promoting effect on the combined drug for killing the Beauveria bassiana with fludioxonil.
Comparative example 3
Carbendazim was purchased from guang agrichemical incorporated, Sichuan.
Application group:
experimental group 1: preparing a solution containing 0.5% of carbendazim by mass concentration;
experimental group 2: preparing a solution containing 0.1% of carbendazim by mass concentration;
experimental group 3: preparing a solution containing fludioxonil with the mass concentration of 0.05%;
experimental group 4: a solution containing 0.05% of carbendazim and 0.05% of fludioxonil was prepared.
Experimental methods
The beauveria bassiana bacterial liquid (bacterial liquid concentration: 1.0 × 10)7Piece/ml) 200 mul are respectively and uniformly mixed with 200 mul of solution prepared by each experimental group of the comparative example 3, after 25 minutes, the mixture is centrifuged at 4000rpm for 5 minutes, upper liquid is sucked away to stop disinfection, equal amount of water is added to resuspend thalli, the surfaces of the silkworm bodies after 2 years are coated until the silkworm bodies are wet, after a culture dish cover is internally attached with water-dipping filter paper for moisturizing and breeding for 24 hours, the normal mulberry leaf breeding is corrected, the survival rate is investigated after two years, and the survival rate is calculated by the following calculation formula:
the calculation results are shown in table 8;
TABLE 8 influence of carbendazim and its disinfectant in combination with fludioxonil on the survival rate of silkworms
| Group of | Survival rate of silkworm% |
| Experimental group 1 | 71.8 |
| Experimental group 2 | 7.7 |
| Experimental group 3 | 82.2 |
| Experimental group 4 | 86.4 |
The data in table 8 show that carbendazim has a certain killing effect on beauveria bassiana, but the using concentration is high, after 0.5% of carbendazim kills beauveria bassiana, the survival rate of silkworms is 71.8%, after 0.05% of fludioxonil kills beauveria bassiana, the survival rate of the silkworms reaches 82.2%, after 0.1% of carbendazim kills beauveria bassiana, the survival rate of the silkworms is only 7.7%, the survival rate of the silkworms in the experimental group 3 and the experimental group 4 is not greatly different, which shows that the killing effect of the low-concentration carbendazim on beauveria bassiana is very weak, and the combined use of the carbendazim and the fludioxonil has no mutual promotion effect on the killing effect on the beauveria bassiana.
Claims (6)
1. The disinfectant for the silkworms comprises the following raw material components in parts by weight:
4-7 parts of fludioxonil, 3-5 parts of propamocarb hydrochloride and 10000 parts of water; mixing the above raw materials.
2. The disinfectant for silkworms as claimed in claim 1, wherein fludioxonil is in parts by weight: 4 parts, 5 parts, 6 parts or 7 parts.
3. The disinfectant for silkworms as claimed in claim 1, wherein the propamocarb hydrochloride is in parts by weight: 3 parts, 4 parts or 5 parts.
4. The use of the disinfectant for silkworms according to claim 1, characterized by the use of the disinfectant for silkworms in disinfection of rearing beds in rearing rooms.
5. The use of the disinfectant for silkworms according to claim 4, wherein the disinfection step of the rearing room and the rearing instrument is as follows:
the disinfectant for silkworms is added according to the volume of 200-300 mL/m2The spraying amount of the disinfectant is sprayed on silkworm rearing room silkworm rearing instruments or the silkworm rearing instruments are soaked in the disinfectant for silkworms for 20-40 min.
6. The use of the disinfectant for silkworms according to claim 4, characterized in that the rearing bed disinfection step is as follows:
the disinfectant for silkworms is added according to the volume of 200-300 mL/m2The spraying amount is sprayed on the silkworm rearing bed.
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