CN115226730A - Volatile disinfectant for animal house and preparation method thereof - Google Patents
Volatile disinfectant for animal house and preparation method thereof Download PDFInfo
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- CN115226730A CN115226730A CN202211018259.XA CN202211018259A CN115226730A CN 115226730 A CN115226730 A CN 115226730A CN 202211018259 A CN202211018259 A CN 202211018259A CN 115226730 A CN115226730 A CN 115226730A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
- A01N25/28—Microcapsules or nanocapsules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
Abstract
The invention relates to a volatile disinfectant for livestock houses and a preparation method thereof, wherein the volatile disinfectant for livestock houses comprises an acidic solution and a disinfectant powder, and the preparation method comprises the following steps: preparing an acid solution: adding water into acetic acid or peroxyacetic acid to dilute until the volume concentration is 15-20% to obtain the acidic solution; preparing disinfection powder: the disinfection powder is prepared by uniformly mixing quicklime powder, calcium carbonate, bamboo charcoal powder and the like in mass to prepare mixed powder, and adding equal-mass temperature-sensitive traditional Chinese medicine microcapsules into the mixed powder. The acidic solution is matched with the soaked disinfection powder for use, the mode that quicklime powder is directly scattered on the ground is different from the mode that the quicklime powder is directly scattered on the ground, the dispersing with wind is not easy, the adverse effect on the health of human beings and livestock and poultry is avoided, the safety is good, in addition, the disinfectant provided by the invention has a good volatilization effect, and a more lasting and efficient sterilization effect is realized.
Description
Technical Field
The invention belongs to the technical field of livestock house disinfectants, and particularly relates to a volatile disinfectant for livestock houses and a preparation method thereof.
Background
The prepared livestock shed disinfection is an important and necessary part for daily management of the farm, is one of important measures for preventing epidemic disease infection of the farm and controlling epidemic disease outbreak, and is an important guarantee for the efficient development of the farm. The common disinfectant in the breeding industry mainly comprises two main categories of organic disinfectant and inorganic disinfectant;
at present, the disinfection of the livestock house is documented, and a mode of directly spraying quicklime powder to the ground is adopted, but the powder is easy to scatter with the wind, the scattered powder is easy to generate adverse effects on the health of human beings and livestock and poultry, the safety problem exists, the disinfection effect is general, and the stability is poor, so that the development of a novel and effective livestock house disinfectant is crucial;
based on the problems, we propose a volatile disinfectant for animal houses and a preparation method thereof.
Disclosure of Invention
The invention aims to solve the problems and provides a volatile disinfectant for a livestock house and a preparation method thereof.
The invention realizes the purpose through the following technical scheme:
the invention provides a preparation method of a volatile disinfectant for livestock houses, which comprises an acidic solution and a disinfectant powder, and the preparation method comprises the following steps:
(1) Preparation as acidic solution: adding water into acetic acid or peroxyacetic acid to dilute until the volume concentration is 15-20% to obtain the acidic solution;
(2) Preparing disinfection powder: the disinfection powder is prepared by uniformly mixing quicklime powder, calcium carbonate, bamboo charcoal powder and the like in mass to prepare mixed powder, and adding temperature-sensitive traditional Chinese medicine microcapsules with the same mass as the mixed powder into the mixed powder.
In the step (2), the temperature-sensitive traditional Chinese medicine microcapsule is obtained by microencapsulating traditional Chinese medicine volatile oil serving as a core material and a high polymer material with temperature-sensitive performance serving as a wall material.
As a further optimization scheme of the invention, the traditional Chinese medicine volatile oil is a composition of equal-volume and uniform-mixing of the rhizoma atractylodis volatile oil and the mint volatile oil, and the high polymer material with the temperature-sensitive property is a mixture of sodium alginate and polyethylene glycol.
As a further optimization scheme of the invention, the preparation method of the temperature-sensitive traditional Chinese medicine microcapsule comprises the following steps:
(1) Preparing an aqueous solution of sodium alginate and polyethylene glycol, and stirring to uniformly disperse the aqueous solution for later use;
(2) Dissolving the Chinese medicinal volatile oil in ethyl acetate, adding emulsifier and deionized water, and emulsifying to obtain oil/water emulsion;
(3) Extruding the oil/water emulsion obtained in the step (2) from an inner pipe of a concentric discharge port of a feeder, simultaneously extruding the aqueous solution obtained in the step (1) from an outer pipe of the concentric discharge port of the feeder to form a concentric linear continuous fluid, cutting the concentric linear fluid by a rotating blade to form fine particles, then curing in a calcium ion salt solution, and drying to obtain the temperature-sensitive traditional Chinese medicine microcapsule.
The invention also provides the volatile disinfectant for the livestock house prepared by the preparation method.
As a further optimization scheme of the invention, when the volatile disinfectant for the livestock house is used, the disinfectant powder is uniformly covered on the ground of the livestock house, the spraying thickness is 1-2cm, then the acid solution is uniformly sprayed onto the disinfectant powder in a spraying mode until the disinfectant powder is completely soaked, and the using amount of the acid solution is 5ml of acid solution sprayed onto each 1g of disinfectant powder.
The working principle of the invention is as follows: the volatile disinfectant for the animal house comprises an acidic solution and a disinfecting powder, wherein the acidic solution is obtained by diluting acetic acid or peracetic acid with water until the volume concentration is 15-20%, the disinfecting powder is obtained by uniformly mixing quicklime powder, calcium carbonate, bamboo charcoal powder and other mass to prepare mixed powder, and then adding equal mass of temperature-sensitive traditional Chinese medicine microcapsules into the mixed powder, and the acetic acid or peracetic acid has strong volatility and has good effects of sterilizing and disinfecting air.
In conclusion, the beneficial effects of the invention are as follows:
(1) The invention uses the acid solution in combination with the soaking disinfection powder, is different from the mode of directly spraying the quicklime powder on the ground, is not easy to disperse with the wind, avoids the adverse effect on the health of human beings and livestock and poultry, and has good safety;
(2) The disinfectant provided by the invention has a good volatilization effect and a more lasting and efficient sterilization effect.
Detailed Description
The present invention is described in further detail below, and it should be noted that the following detailed description is provided for illustrative purposes only, and is not intended to limit the scope of the present invention, which is defined by the appended claims.
Example 1
The embodiment provides a preparation method of a volatile disinfectant for livestock houses, which comprises an acidic solution and a disinfectant powder, and the preparation method comprises the following steps:
(1) Preparing an acid solution: adding water into acetic acid or peroxyacetic acid to dilute until the volume concentration is 15-20% to obtain the acidic solution;
(2) Preparing temperature-sensitive traditional Chinese medicine microcapsules: the traditional Chinese medicine temperature-sensitive microcapsule is prepared by taking traditional Chinese medicine volatile oil as a core material and taking a high polymer material with temperature-sensitive performance as a wall material and carrying out microencapsulation treatment, wherein the traditional Chinese medicine volatile oil is a composition prepared by uniformly mixing equal parts of rhizoma atractylodis volatile oil and mint volatile oil in equal volume, the high polymer material with temperature-sensitive performance is sodium alginate and polyethylene glycol, and the preparation method specifically comprises the following steps:
preparing 1L of 0.5% sodium alginate and polyethylene glycol solution, wherein the molecular weight of polyethylene glycol is 400kDa, the weight ratio of sodium alginate to polyethylene glycol is 1;
dissolving 4-6g of Chinese medicinal volatile oil in 90-110mL ethyl acetate, adding 0.25-0.5g of emulsifier and 5-10mL water, and emulsifying at high speed by using a high-speed shearing emulsifying machine, wherein the emulsifying speed is 5000-7000r/min, and the emulsifying time is 10-30min to form an oil/water emulsion;
extruding the oil/water emulsion from an inner pipe of a concentric discharge port of a feeder, simultaneously extruding the water solution of sodium alginate and polyethylene glycol from an outer pipe of the concentric discharge port of the feeder to form a concentric linear continuous fluid, cutting the concentric linear fluid by a rotating blade to form fine particles, then curing in a calcium chloride solution, and drying to obtain the temperature-sensitive traditional Chinese medicine microcapsule.
(3) Preparing disinfection powder: and (3) adding uniformly mixed powder of quicklime powder, calcium carbonate, bamboo charcoal powder and the like in parts by mass into the temperature-sensitive traditional Chinese medicine microcapsule prepared in the step (2) to obtain the disinfection powder.
Example 2
The embodiment provides a preparation method of a volatile disinfectant for livestock houses, which comprises an acidic solution and a disinfectant powder, and the preparation method comprises the following steps:
(1) Preparing an acid solution: adding water into the peroxyacetic acid to dilute the peroxyacetic acid until the volume concentration is 20% so as to obtain the acidic solution;
(2) Preparing temperature-sensitive traditional Chinese medicine microcapsules: the traditional Chinese medicine volatile oil is taken as a core material, a high polymer material with temperature-sensitive performance is taken as a wall material, and the traditional Chinese medicine volatile oil is obtained by microencapsulation treatment, wherein the traditional Chinese medicine volatile oil is a composition of the rhizoma atractylodis volatile oil and the mint volatile oil which are uniformly mixed in equal volume, the high polymer material with the temperature-sensitive performance is sodium alginate and polyethylene glycol, and the preparation method comprises the following specific steps:
preparing 1L of 0.5% sodium alginate and polyethylene glycol solution, wherein the molecular weight of the polyethylene glycol is 400kDa, and the weight ratio of the sodium alginate to the polyethylene glycol is 1. Stirring and mixing uniformly, removing bubbles, and placing in a container connected with an outer pipe of the concentric discharge port for later use;
dissolving 5g of traditional Chinese medicine volatile oil in 90mL of ethyl acetate, adding 0.5g of emulsifier and 10mL of water, and emulsifying at high speed by using a high-speed shearing emulsifying machine, wherein the emulsifying speed is 5000r/min, and the emulsifying time is 30min to form oil/water emulsion;
extruding the oil/water emulsion from an inner pipe of a concentric discharge port of a feeder, simultaneously extruding the water solution of sodium alginate and polyethylene glycol from an outer pipe of the concentric discharge port of the feeder to form a concentric linear continuous fluid, cutting the concentric linear fluid by a rotating blade to form fine particles, then curing in a calcium chloride solution, and drying to obtain the temperature-sensitive traditional Chinese medicine microcapsule.
(3) Preparing disinfection powder: the disinfection powder is prepared by uniformly mixing quicklime powder, calcium carbonate, bamboo charcoal powder and the like by mass to prepare mixed powder, and adding equal-mass temperature-sensitive traditional Chinese medicine microcapsules into the mixed powder.
2. Proof test
1. In order to explore the synergy between the acidic solution and the disinfection powder, the formula of the components disclosed in the embodiment 2 is used, the dosage of the mixed powder is kept to be constant, and the disinfection powder A is obtained by mixing the mixed powder A which is uniformly mixed by the mass of quicklime powder, calcium carbonate, bamboo charcoal powder and the like with the temperature-sensitive traditional Chinese medicine microcapsules of the same mass;
the acidic solution disclosed in example 2 was used as example 1 in an amount of 5ml per 1g of disinfectant powder A;
furthermore, 5ml of water was used per 1g of the disinfectant powder A as comparative example 1, and 5ml of 84 disinfectant solution having a volume concentration of 20% was used per 1g of the disinfectant powder A as comparative example 2.
Dividing the livestock shed into 3 subareas of 100cm multiplied by 100cm at random in an equal way in a livestock farm, uniformly scattering 500g of the disinfection powder A and a control group in 3 groups on each subarea, spraying the disinfection powder A and the control group with the thickness of 1cm, uniformly spraying the acid solution on the disinfection powder in the form of spray in the example 1 and the comparative example 1-2 until the disinfection powder is completely soaked, and finishing the disinfection step;
before the disinfection step, equally dividing each subarea into 25 small subareas of 20cm multiplied by 20cm, wiping two reserved samples of each small subarea by using a cotton swab, carrying out microbial culture on one reserved sample of each small subarea under the same microbial culture condition, counting the total bacterial colony number of the reserved samples of each small subarea by using a colony counter, and summing up to obtain 100cm bacterial colony number 2 The total number of bacterial colonies in the sample was averaged to obtain the total count per 1cm 2 The total bacterial colony number in the small subarea is measured by MPN method to obtain total coliform number of 100cm 2 Total number of bacterial colonies within.
And 2h after disinfection, counting the total number of bacterial colonies and the total number of escherichia coli according to the same operation before disinfection to obtain a final value, counting the sterilization rate, and evaluating the disinfection effect, wherein the result is shown in table 1.
TABLE 1 statistical table of measurement results
The results in table 1 show that when the disinfection powder A obtained by mixing the mixed powder A obtained by uniformly mixing the quicklime powder, the calcium carbonate, the bamboo charcoal powder and the like with the temperature-sensitive traditional Chinese medicine microcapsules with equal mass is matched with an acid solution for use, the disinfection powder A has better sterilization rate for bacteria and escherichia coli and has the best disinfection effect.
2. In order to explore the influence of the temperature-sensitive traditional Chinese medicine microcapsules on the antibacterial effect of the disinfectant, the traditional Chinese medicine volatile oil which is not subjected to microencapsulation treatment is taken as a comparative example 3, and then the mixed powder component A with the same mass is taken and doped into the comparative example 3 with the same mass to obtain the disinfection powder B.
Before the disinfection step, equally dividing each subarea into 25 small subareas of 20cm multiplied by 20cm, wiping two reserved samples of each small subarea by using a cotton swab, carrying out microbial culture on one reserved sample of each small subarea under the same microbial culture condition, counting the total bacterial colony number of the reserved samples of each small subarea by using a colony counter, and summing up to obtain 100cm bacterial colony number 2 The total number of bacterial colonies in the sample was averaged to obtain 1cm per sample 2 The total bacterial colony number in the small subarea is measured by MPN method, and the total number of coliform colony is 100cm before disinfection 2 The total number of bacterial colonies in the water treatment system is counted, then the same amount of the disinfection powder A and the same amount of the disinfection powder B are respectively and uniformly sprayed on each partition, the spraying thickness is 1cm, then the acid solution given in the example 2 is uniformly sprayed on each partition until the disinfection powder of each partition is soaked, the disinfection step is completed, and the amount of the acid solution on each partition is 3 times of the mass of the disinfection powder.
According to 1h, 2h and 5h after disinfection, the total number of bacterial colonies and the total number of escherichia coli are counted by the same operation before disinfection, after a final value is obtained, the sterilization rate is counted, the disinfection effect is evaluated, and the result is shown in table 2.
TABLE 2 statistical table of measurement results
The results in table 2 show that when the disinfection powder A obtained by mixing the mixed powder A obtained by uniformly mixing the quicklime powder, the calcium carbonate, the bamboo charcoal powder and the like with the temperature-sensitive traditional Chinese medicine microcapsule with equal mass is matched with an acid solution for use, the disinfection powder A has a more lasting disinfection effect on bacteria and escherichia coli and has a long-acting sterilization effect.
While the foregoing description shows and describes several preferred embodiments of the invention, it is to be understood, as noted above, that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. A preparation method of a volatile disinfectant for a livestock house is characterized in that the volatile disinfectant for the livestock house comprises an acidic solution and a disinfectant powder, and the preparation method comprises the following steps:
(1) Preparing an acid solution: adding water into acetic acid or peroxyacetic acid to dilute until the volume concentration is 15-20% to obtain the acidic solution;
(2) Preparing disinfection powder: the disinfection powder is prepared by uniformly mixing quicklime powder, calcium carbonate, bamboo charcoal powder and the like in mass to prepare mixed powder, and adding temperature-sensitive traditional Chinese medicine microcapsules with the same mass as the mixed powder into the mixed powder.
2. The method for preparing a volatile disinfectant for animal houses according to claim 1, wherein in the step (2), the temperature-sensitive traditional Chinese medicine microcapsule is obtained by microencapsulating traditional Chinese medicine volatile oil serving as a core material and a high polymer material with temperature-sensitive property serving as a wall material.
3. The preparation method of the volatile disinfectant for the livestock house according to claim 2, characterized in that the traditional Chinese medicine volatile oil is a composition of rhizoma atractylodis volatile oil and mint volatile oil which are uniformly mixed in equal volume, and the high polymer material with the temperature-sensitive property is a mixture of sodium alginate and polyethylene glycol.
4. The preparation method of the volatile disinfectant for the animal house according to claim 3, wherein the preparation method of the temperature-sensitive traditional Chinese medicine microcapsule comprises the following steps:
(1) Preparing an aqueous solution of sodium alginate and polyethylene glycol, and stirring to uniformly disperse the aqueous solution for later use;
(2) Dissolving the Chinese medicinal volatile oil in ethyl acetate, adding emulsifier and deionized water, and emulsifying to obtain oil/water emulsion;
(3) Extruding the oil/water emulsion obtained in the step (2) from an inner pipe of a concentric discharge port of a feeder, simultaneously extruding the aqueous solution obtained in the step (1) from an outer pipe of the concentric discharge port of the feeder to form concentric linear continuous fluid, cutting the concentric linear fluid by a rotating blade to form fine particles, then curing in calcium ion salt solution, and drying to obtain the temperature-sensitive traditional Chinese medicine microcapsule.
5. A volatile disinfectant for livestock houses prepared by the preparation method as claimed in any one of claims 1-4.
6. The volatile disinfectant for livestock houses as claimed in claim 5, wherein when the volatile disinfectant for livestock houses is used, the disinfectant powder is uniformly covered on the ground of the livestock houses, the thickness of the disinfectant powder is 1-2cm, and then the acidic solution is uniformly sprayed onto the disinfectant powder in the form of spray until the disinfectant powder is completely soaked, wherein the amount of the acidic solution is 5ml for every 1g of the disinfectant powder.
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Cited By (1)
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| CN115885991A (en) * | 2022-11-08 | 2023-04-04 | 四川成康动物药业有限公司 | Disinfectant composition and production process thereof |
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