CN109699686A - A kind of disinfectant for killing avian influenza virus - Google Patents
A kind of disinfectant for killing avian influenza virus Download PDFInfo
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Abstract
The invention discloses a kind of disinfectants for killing avian influenza virus, raw material forms according to the following ratio: 30wt% hydrogen peroxide 15-25 parts by volume, 20wt% Peracetic acid 2-6 parts by volume, sodium pyrophosphate 0.5-2.5 mass parts, 40wt% dodecyl dimethyl benzyl ammonium bromide 1-3 parts by volume, sodium bicarbonate 1-3 mass parts, polyoxyethylene alkyl ether phosphate 1-4 mass parts, NaTDC 0.1-0.3 mass parts, nano zine oxide 2-4 mass parts, 75wt% ethyl alcohol 15-25 parts by volume, deionized water 80-100 parts by volume, wherein corresponding 1 parts by volume of 1 mass parts, 1g corresponds to 1mL.The disinfectant prepared by the present invention for killing avian influenza virus, thorough disinfection and sterilizing are carried out to poultry She Peng ground wall and excreta, bacterium and the virus of poultry cultivation field can be killed, source control to avian influenza virus, furthermore present invention may also apply to the public places such as school, station, hospital and family place, anti-fowl influenza virus, disinfection speed is fast and dosage is low, killing ability is strong, acting duration is long, free from environmental pollution.
Description
Technical field
The invention belongs to disinfectant technical fields, and in particular to a kind of disinfectant for killing avian influenza virus.
Background technique
In recent years, highly pathogenic bird flu not only causes huge economic loss to the poultry breeding industry in China, but also to public affairs
Safe and healthy bring seriously threatens altogether.Avian influenza virus is according to its surface glycoprotein hemagglutinin (HA) and neuraminidase (NA)
Antigenic specificity can be divided into 16 HA hypotypes (H1-H16) and 9 NA hypotypes (N1-N9).H5N1 and H9N2 hypotype in 1997
Bird flu, H5N1 bird flu in 2004 spread Asia again, and China finder in 2013 infects H7N9 avian influenza virus, and 2014
There is the first people's H5N6 subtype avian influenza case in China.The variation of HA and NA gene is the main reason for leading to Influenza Outbreak, and
Avian influenza virus can be entered human body by alimentary canal and respiratory tract and be transmitted to people, and the mankind directly contact by avian flu virus infection
Poultry and its excrement or directly contact avian influenza virus can also be infected, and be also to pass by droplet and contact respiratory secretions
Broadcast approach.
The aggregation ground of birds is the place that virus is most easily hidden, and takes the main biography that viruliferous birds are people infected with bird flus
Dye source, the avian influenza virus propagation reduced between control birds, especially poultry is particularly important, therefore carries out spraying disinfection with fowl
The pathogenic microorganism for killing invasion in time is sought to, epidemic disease hidden danger is eliminated.Being with the effect that fowl carries out spraying disinfection can be direct
Killing is hidden in birds aggregation ground environment, the pathogenic microorganism including air, and it is shallow directly to kill carcass table, respiratory tract
The microorganism that table is detained.Currently used air disinfection method is chemicals, including formaldehyde, glutaraldehyde, ethylene oxide, peroxide
The chemical methodes such as acetic acid, hydrogen peroxide, chlorine dioxide, chlorine, copper sulphate, quick lime, ethyl alcohol and ultraviolet light irradiation, ozone hair
The physical methods such as raw device.
Patent 200710039444.6 disclose it is a kind of with pure Chinese medicine anti-avian influenza air disinfectant and preparation method thereof,
The component and weight ratio of pure Chinese medicine anti-avian influenza air disinfectant are as follows: 1~10 part of hypericum perforatum, 1~10 part of radix scutellariae, isatis root 1
~10 parts, 1~10 part of honeysuckle, 1~10 part of wrinkled giant hyssop, 1~10 part of eupatorium.The Chinese medicine anti-avian influenza air disinfectant is non-stimulated
Property, no toxicity integrates disinfection and fresh air, is suitble to use on various occasions, but the bird flu disinfectant kills
Virus of going out takes a long time, and dosage is big, and the dosage for being difficult to meet market is small, the short requirement of antivirus time.
Patent 200910263970.X discloses a kind of compound disinfector of peroxyacetic acid and glutaric dialdehyde, and the disinfectant is by following original
Material proportionally forms: 75~90 parts by volume of Peracetic acid, 10~23 parts by volume of hydrogen peroxide, 0.2~3 parts by volume of glutaraldehyde,
0.1~3 mass parts of 0.5~5 parts by volume of polyoxyethylene aliphatic alcohol ether and sodium pyrophosphate, wherein 1 mass parts correspond to 1g pairs of 1 parts by volume
Answer 1ml.The disinfectant work fast, killing power is strong, acting duration is long, small toxicity is non-corrosive, Recipe is simple,
But glutaraldehyde is to human skin, mucous membrane is irritating and solidification, and can make one sensitization, furthermore the disinfectant is unstable easily point
It solves and is affected by organic matter, when being used for coop sterilizing, the remaining chicken manure of henhouse can be such that the sterilization of the disinfectant makees
With being substantially reduced, disinfection is caused to be not thorough.
Therefore bird flu disinfectant is developed, is the effective way for solving poultry-farm, China prevention and control of fowl influenza.Develop a fowl
Influenza disinfectant, reaches that disinfection speed is fast and dosage is low, killing ability is strong, acting duration is long, small toxicity is corrosion-free
The requirement of property, stability and high efficiency.
Summary of the invention
The purpose of the present invention is to provide a kind of disinfectants for killing avian influenza virus.
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 15-25 body
Product part, 20wt% Peracetic acid 2-6 parts by volume, sodium pyrophosphate 0.5-2.5 mass parts, 40wt% dodecyl dimethyl benzyl bromide
Change ammonium 1-3 parts by volume, sodium bicarbonate 1-3 mass parts, polyoxyethylene alkyl ether phosphate 1-4 mass parts, NaTDC 0.1-
0.3 mass parts, nano zine oxide 2-4 mass parts, 75wt% ethyl alcohol 15-25 parts by volume, deionized water 80-100 parts by volume, wherein
1 mass parts correspond to 1 parts by volume, and 1g corresponds to 1mL.
The pH value of the disinfectant for killing avian influenza virus is 6.0-8.0.
Preferably, the raw material of the disinfectant for killing avian influenza virus further includes attapulgite clay powder 4-8 mass parts.
Preferably, the raw material of the disinfectant for killing avian influenza virus further includes wormwood essential oil 4-8 parts by volume.
Preferably, the raw material of the disinfectant for killing avian influenza virus further includes wormwood essential oil 4-8 mass parts.
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 40-50 parts by volume being imported in mixing and stirring tank, in tank
15-45 DEG C of temperature, 30wt% hydrogen peroxide 15-25 parts by volume is imported in blending tank and is mixed with deionized water, and is stirred evenly
Hydrogen peroxide is made, in sequence respectively by 75wt% ethyl alcohol 15-25 parts by volume, polyoxyethylene alkyl ether phosphate 1-4 mass parts,
40wt% dodecyl dimethyl benzyl ammonium bromide 1-3 parts by volume, 20wt% Peracetic acid 2-6 parts by volume import in blending tank,
Revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 40-50 parts by volume being imported in mixing and stirring tank,
15-45 DEG C of temperature in tank, by sodium pyrophosphate 0.5-2.5 mass parts, nano zine oxide 2-4 mass parts, sodium bicarbonate 1-3 mass
Part, NaTDC 0.1-0.3 mass parts are added in mixing and stirring tank, and revolving speed 1000r/min is uniformly mixed and is configured to inorganic salts
Solution for standby；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 2-4 times, homogenization pressure 15-
25MPa, obtains disinfectant for treating fowl flu by 15-45 DEG C of homogenizing temperature.
Preferably, attapulgite clay powder 4-8 mass parts or wormwood essential oil 4-8 parts by volume can also be added in the step (2)
Or nanometer Cu2Zn(PO4)(OH)3(H2O)2Powder 4-8 mass parts.
The effect of feed components is as follows:
Hydrogen peroxide is a kind of strong oxidizer, and aqueous solution is commonly called as hydrogen peroxide, is colourless transparent liquid, can kill one and chop up
Bacterium brood body, virus, fungi and its spore etc. also have certain killing effect to bacterial spore, have wide spectrum, efficient, quick-acting, nothing
The feature of poison.
Peracetic acid is strong oxidizer, can kill all microorganisms, can be rapidly to virus, bacterium, fungi and gemma
It kills, can be widely applied to various utensils and environment disinfected.0.2% solution contacts 10 minutes can reach sterilizing purpose substantially.With
In air, environment disinfected, prevention disinfection, has the characteristics that wide spectrum, quick-acting, efficient.
Dodecyl dimethyl benzyl ammonium bromide is one of cationic surfactant, there is clean, sterilizing to make
With, be widely used in sterilization, disinfection, anti-corrosion, emulsification, de-sludging, solubilising etc., can kill bacterial propagule and lipophilic virus, matter
Stablize, fast light, heat-resisting, non-volatility, to non-stimulated, the no cumulative toxicity of tissue, under the dosage of 10mg/L effective substance, this product
Killing rate to heterotroph is 98.9%.
Ethyl alcohol is good organic solvent, can dissolve hydrogen peroxide, Peracetic acid, dodecyl dimethyl benzyl bromide
Change the organic matters such as ammonium, be furthermore possible to kill the microorganisms such as mycobacteria, fungi, virus and bacterial propagule, reaches disinfection and require
, have the characteristics that quick-acting and nontoxic.
Nano zine oxide is a kind of high-end high function fine inorganic product, has efficient biological activity, absorptivity
The characteristics such as high, the strong, safety and stability of oxidation resistance.Its large specific surface area, chemical activity are high, product fineness, chemical purity and grain
Sub- shape, which can according to need, to be adjusted, and is had photochemical effect and preferably covered imparted energy, ultraviolet light
Screening rate is up to 98%, meanwhile, it also has a series of special performances such as antibacterial bacteriostatic, the anti-enzyme of taste of dispelling, as strong oxidizer
The degradation to organic matter is completed, germs and viruses are killed, it is obvious to avian influenza virus antivirus effect.
Sodium pyrophosphate, sodium bicarbonate, polyoxyethylene alkyl ether phosphate, NaTDC are hydrogen peroxide, Peracetic acid
A stable environment is provided with dodecyl dimethyl benzyl ammonium bromide, the component with disinfection and sterilization ingredient is played and makees
With guarantee antivirus acting duration length, stability and high efficiency.
Beneficial effects of the present invention:
Bird flu disinfectant prepared by the present invention carries out thorough disinfection to poultry She Peng ground wall and excreta and goes out
Bacterium can kill bacterium and the virus of poultry cultivation field, control the source of avian influenza virus, furthermore present invention may also apply to learn
The public places such as school, station, hospital and family place can kill the pathogenicities such as bacterium, the virus in body surface, article and air
Microorganism, anti-fowl influenza virus, disinfection speed is fast and dosage is low, killing ability is strong, acting duration is long, small toxicity
It is non-corrosive, stability and high efficiency, free from environmental pollution.
Specific embodiment
The present invention will be further described combined with specific embodiments below.
Embodiment 1
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 20L,
20wt% Peracetic acid 4L, sodium pyrophosphate 1.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 2L, sodium bicarbonate 2kg,
Polyoxyethylene alkyl ether phosphate 2.5kg, NaTDC 0.2kg, nano zine oxide 3kg, 75wt% ethyl alcohol 20L, deionization
Water 90L.
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature 30 in tank
DEG C, 30wt% hydrogen peroxide 20L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 20L, polyoxyethylene alkyl ether phosphate 2.5kg, 40wt% dodecyl dimethyl Benzylphosphonium Bromide
Ammonium 2L, 20wt% Peracetic acid 4L is imported in blending tank, revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature in tank
30 DEG C, mixing and stirring tank is added in sodium pyrophosphate 1.5kg, nano zine oxide 3kg, sodium bicarbonate 2kg, NaTDC 0.2kg
In, revolving speed 1000r/min is uniformly mixed that be configured to inorganic salt solution spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 3 times, homogenization pressure 20MPa,
30 DEG C of matter temperature, obtain disinfectant for treating fowl flu.
Embodiment 2
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 15L,
20wt% Peracetic acid 2L, sodium pyrophosphate 0.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 1L, sodium bicarbonate 1kg,
Polyoxyethylene alkyl ether phosphate 1kg, NaTDC 0.1kg, nano zine oxide 2kg, 75wt% ethyl alcohol 15L, deionized water
80L。
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 40L being imported in mixing and stirring tank, temperature 15 in tank
DEG C, 30wt% hydrogen peroxide 15L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 15L, polyoxyethylene alkyl ether phosphate 1kg, 40wt% dodecyl dimethyl benzyl ammonium bromide
1L, 20wt% Peracetic acid 2L are imported in blending tank, revolving speed 600r/min, are uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 40L being imported in mixing and stirring tank, temperature in tank
15 DEG C, mixing and stirring tank is added in sodium pyrophosphate 0.5kg, nano zine oxide 2kg, sodium bicarbonate 1kg, NaTDC 0.1kg
In, revolving speed 1000r/min is uniformly mixed that be configured to inorganic salt solution spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 2 times, homogenization pressure 15MPa,
15 DEG C of matter temperature, obtain disinfectant for treating fowl flu.
Embodiment 3
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 25L,
20wt% Peracetic acid 6L, sodium pyrophosphate 2.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 3L, sodium bicarbonate 3kg,
Polyoxyethylene alkyl ether phosphate 4kg, NaTDC 0.3kg, nano zine oxide 4kg, 75wt% ethyl alcohol 25L, deionized water
100L。
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 50L being imported in mixing and stirring tank, temperature 45 in tank
DEG C, 30wt% hydrogen peroxide 25L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 25L, polyoxyethylene alkyl ether phosphate 4kg, 40wt% dodecyl dimethyl benzyl ammonium bromide
3L, 20wt% Peracetic acid 6L are imported in blending tank, revolving speed 600r/min, are uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 50L being imported in mixing and stirring tank, temperature in tank
45 DEG C, mixing and stirring tank is added in sodium pyrophosphate 2.5kg, nano zine oxide 4kg, sodium bicarbonate 3kg, NaTDC 0.3kg
In, revolving speed 1000r/min is uniformly mixed that be configured to inorganic salt solution spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 4 times, homogenization pressure 25MPa,
Matter temperature 45 C, obtains disinfectant for treating fowl flu.
Embodiment 4
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 20L,
20wt% Peracetic acid 4L, sodium pyrophosphate 1.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 2L, sodium bicarbonate 2kg,
Polyoxyethylene alkyl ether phosphate 2.5kg, NaTDC 0.2kg, nano zine oxide 3kg, 75wt% ethyl alcohol 20L, concave convex rod
Clay powder 6kg, deionized water 90L.
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature 30 in tank
DEG C, 30wt% hydrogen peroxide 20L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 20L, polyoxyethylene alkyl ether phosphate 2.5kg, 40wt% dodecyl dimethyl Benzylphosphonium Bromide
Ammonium 2L, 20wt% Peracetic acid 4L is imported in blending tank, revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature in tank
30 DEG C, by sodium pyrophosphate 1.5kg, nano zine oxide 3kg, sodium bicarbonate 2kg, NaTDC 0.2kg, attapulgite clay powder
6kg is added in mixing and stirring tank, revolving speed 1000r/min, is uniformly mixed that be configured to inorganic salt solution spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 3 times, homogenization pressure 20MPa,
30 DEG C of matter temperature, obtain disinfectant for treating fowl flu.
Embodiment 5
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 20L,
20wt% Peracetic acid 4L, sodium pyrophosphate 1.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 2L, sodium bicarbonate 2kg,
Polyoxyethylene alkyl ether phosphate 2.5kg, NaTDC 0.2kg, nano zine oxide 3kg, 75wt% ethyl alcohol 20L, wormwood essence
Oily 6L, deionized water 90L.
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature 30 in tank
DEG C, 30wt% hydrogen peroxide 20L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 20L, polyoxyethylene alkyl ether phosphate 2.5kg, 40wt% dodecyl dimethyl Benzylphosphonium Bromide
Ammonium 2L, 20wt% Peracetic acid 4L is imported in blending tank, revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature in tank
30 DEG C, sodium pyrophosphate 1.5kg, nano zine oxide 3kg, sodium bicarbonate 2kg, NaTDC 0.2kg, wormwood essential oil 6L are added
In mixing and stirring tank, revolving speed 1000r/min is uniformly mixed that be configured to inorganic salt solution spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 3 times, homogenization pressure 20MPa,
30 DEG C of matter temperature, obtain disinfectant for treating fowl flu.
Embodiment 6
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 20L,
20wt% Peracetic acid 4L, sodium pyrophosphate 1.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 2L, sodium bicarbonate 2kg,
Polyoxyethylene alkyl ether phosphate 2.5kg, NaTDC 0.2kg, nano zine oxide 3kg, 75wt% ethyl alcohol 20L, nanometer
Cu2Zn(PO4)(OH)3(H2O)2Powder 6kg, deionized water 90L.
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature 30 in tank
DEG C, 30wt% hydrogen peroxide 20L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 20L, polyoxyethylene alkyl ether phosphate 2.5kg, 40wt% dodecyl dimethyl Benzylphosphonium Bromide
Ammonium 2L, 20wt% Peracetic acid 4L is imported in blending tank, revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature in tank
30 DEG C, by sodium pyrophosphate 1.5kg, nano zine oxide 3kg, sodium bicarbonate 2kg, NaTDC 0.2kg, nanometer Cu2Zn(PO4)
(OH)3(H2O)2Powder 6kg is added in mixing and stirring tank, revolving speed 1000r/min, is uniformly mixed that be configured to inorganic salt solution spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 3 times, homogenization pressure 20MPa,
30 DEG C of matter temperature, obtain disinfectant for treating fowl flu.
Embodiment 7
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 20L,
20wt% Peracetic acid 4L, sodium pyrophosphate 1.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 2L, sodium bicarbonate 2kg,
Polyoxyethylene alkyl ether phosphate 2.5kg, NaTDC 0.2kg, nano zine oxide 3kg, 75wt% ethyl alcohol 20L, yellow hair wormwood artemisia
Volatile oil 6L, deionized water 90L.
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature 30 in tank
DEG C, 30wt% hydrogen peroxide 20L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 20L, polyoxyethylene alkyl ether phosphate 2.5kg, 40wt% dodecyl dimethyl Benzylphosphonium Bromide
Ammonium 2L, 20wt% Peracetic acid 4L is imported in blending tank, revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature in tank
30 DEG C, by sodium pyrophosphate 1.5kg, nano zine oxide 3kg, sodium bicarbonate 2kg, NaTDC 0.2kg, yellow hair wormwood artemisia volatile oil 6L
It is added in mixing and stirring tank, revolving speed 1000r/min, is uniformly mixed that be configured to inorganic salt solution spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 3 times, homogenization pressure 20MPa,
30 DEG C of matter temperature, obtain disinfectant for treating fowl flu.
Embodiment 8
A kind of disinfectant for killing avian influenza virus, raw material form according to the following ratio: 30wt% hydrogen peroxide 20L,
20wt% Peracetic acid 4L, sodium pyrophosphate 1.5kg, 40wt% dodecyl dimethyl benzyl ammonium bromide 2L, sodium bicarbonate 2kg,
Polyoxyethylene alkyl ether phosphate 2.5kg, NaTDC 0.2kg, nano zine oxide 3kg, 75wt% ethyl alcohol 20L, nanometer
(Cu1.75Zn0.25)Zn(PO4)(OH)3(H2O)2Powder 6kg, deionized water 90L.
The preparation method of the disinfectant of above-mentioned killing avian influenza virus carries out in accordance with the following steps:
(1) it prepares organic liquor: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature 30 in tank
DEG C, 30wt% hydrogen peroxide 20L is imported in blending tank and is mixed with deionized water, and stirs evenly and hydrogen peroxide is made, according to suitable
Sequence is respectively by 75wt% ethyl alcohol 20L, polyoxyethylene alkyl ether phosphate 2.5kg, 40wt% dodecyl dimethyl Benzylphosphonium Bromide
Ammonium 2L, 20wt% Peracetic acid 4L is imported in blending tank, revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 45L being imported in mixing and stirring tank, temperature in tank
30 DEG C, by sodium pyrophosphate 1.5kg, nano zine oxide 3kg, sodium bicarbonate 2kg, NaTDC 0.2kg, nanometer
(Cu1.75Zn0.25)Zn(PO4)(OH)3(H2O)2Powder 6kg is added in mixing and stirring tank, revolving speed 1000r/min, is uniformly mixed and prepares
It is spare at inorganic salt solution；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously mixed
It is uniform to obtain disinfectant semi-finished product, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 3 times, homogenization pressure 20MPa,
30 DEG C of matter temperature, obtain disinfectant for treating fowl flu.
Experimental example 1:
The disinfectant of the killing avian influenza virus prepared according to the preparation method of embodiment 1-8 is sample, verifies bird flu
Disinfectant is killed avian influenza virus and is tested.
Avian influenza virus selection: H5N6 subtype avian influenza virus allantoic fluid is placed in -70 DEG C of preservations, measures chicken embryo before testing
Median infective dose (EID50) it is 107/0.1ml。
Chicken manure: it is derived from experiment chicken house；Tap water: it is derived from running water pipe, PH6.8-7.0.
It prepares the suspension of avian influenza virus and chicken manure: virus first being carried out 1:1000 times and is diluted, take the diluted fowl stream of 100ml
Influenza Virus is added chicken manure 50g, is configured to the suspension of avian influenza virus and chicken manure.
1:50 times, 1 are diluted to tap water respectively according to bird flu disinfectant prepared by the preparation method of embodiment 1-8:
100 times, 1:500 times, 1:1000 times, 1:2000 times, 1:4000 times dilute, respectively with the suspension of avian influenza virus and chicken manure 25
The suspension volume ratio that 20min, thimerosal and avian influenza virus and chicken manure are acted at a temperature of DEG C is 9:1, and it is dense to count different dilutions
The disinfectant of degree kills being repeated 3 times as a result, testing for virus, as a result referring to table 1.
1 bird flu disinfectant of table is to avian influenza virus killing rate (%) (n=3)
Note: * represents P < 0.05 compared with example 1 group
As can be seen from Table 1, when the extension rate of bird flu disinfectant is 1:50 and 1:100 times, the virus of embodiment 1-8
Killing rate is 100%, and there was no significant difference.When the extension rate of bird flu disinfectant is 1:500,1:1000 and 1:2000 times,
Embodiment 1-3, the viral killing rate of embodiment 7 are suitable, and there was no significant difference；The viral killing rate of embodiment 4-6, embodiment 8
It is significantly higher than embodiment 1, viral killing rate is 90% or more.When the extension rate of bird flu disinfectant is 1:4000 times, implement
Example 1-3, embodiment 7 viral killing rate be 0, there was no significant difference, illustrate that the concentration of disinfectant is too small, virus can not be killed,
Thimerosal failure；Embodiment 4-6, embodiment 8 viral killing rate be significantly higher than embodiment 1, viral killing rate 50% or so,
Illustrate embodiment 4-6, the disinfectant of embodiment 8 still has certain antivirus effect.
Experimental example 2:
It is sample according to bird flu disinfectant prepared by the preparation method of embodiment 1-8, verifies bird flu disinfectant to chicken
The toxicity test of embryo, as a result referring to table 1.
Experimental animal: 10 age in days SPF chicken embryos.Tap water: it is derived from running water pipe, PH6.8-7.0.
1:30 times, 1 are diluted to tap water respectively according to bird flu disinfectant prepared by the preparation method of embodiment 1-8:
40 times, 1:50 times, 1:100 times, 1:500 times, 1:1000 times dilute, respectively inoculated into chick embryo, and each dilution is inoculated with 10 pieces of chicken embryos,
Inoculum concentration is 0.2ml/ chicken embryo, and the disinfectant that different diluted concentrations are counted after 96h repeats the toxicity test of chicken embryo as a result, testing
3 times, as a result referring to table 2.
Toxicity test result (n=3) of the 2 bird flu disinfectant of table to chicken embryo
Note: * represents P < 0.05 compared with example 1 group
As can be seen from Table 2, when the extension rate of bird flu disinfectant is 1:30, the chicken embryo of embodiment 1-3, embodiment 7
There was no significant difference for survival volume, and chicken embryo has death condition, illustrates that the bird flu disinfectant of the embodiment is toxic to chicken embryo, implements
Example 4-6, embodiment 8 chicken embryo survival volume be significantly higher than embodiment 1, illustrate the bird flu disinfectant of the embodiment in the dilution times
It is several lower toxic to chicken embryo, but toxicity is low compared with embodiment 1；When the extension rate of bird flu disinfectant is 1:40, embodiment 1-
3, there was no significant difference for the chicken embryo survival volume of embodiment 7, and chicken embryo has death condition, illustrates that the bird flu disinfectant of the embodiment exists
It is toxic to chicken embryo under the extension rate, embodiment 4-6, embodiment 8 chicken embryo survival volume be significantly higher than embodiment 1, chicken embryo is deposited
Amount living is 10, illustrates the bird flu disinfectant of the embodiment to chicken embryo nontoxicity；The extension rate of bird flu disinfectant be 1:50,
When 1:100,1:500,1:1000, there was no significant difference for the chicken embryo survival volume of embodiment 1-8, illustrates that the fowl of the embodiment flows this and disappears
Toxic agent is under the extension rate to chicken embryo nontoxicity.The maximal non-toxic dosage for sum up illustrating the disinfectant is 1:50 times of dilution.
Experimental example 3:
It is sample according to bird flu disinfectant prepared by the preparation method of embodiment 1-8, verifies bird flu disinfectant to fowl
The time test that influenza virus is killed.
Avian influenza virus selection: H5N6 subtype avian influenza virus allantoic fluid is placed in -70 DEG C of preservations, measures chicken embryo before testing
Median infective dose (EID50) it is 107/0.1ml。
Chicken manure: it is derived from experiment chicken house；Tap water: it is derived from running water pipe, PH6.8-7.0.
It prepares the suspension of avian influenza virus and chicken manure: virus first being carried out 1:1000 times and is diluted, take the diluted fowl stream of 100ml
Influenza Virus is added chicken manure 50g, is configured to the suspension of avian influenza virus and chicken manure.
1:50 times and 1 are diluted to tap water respectively according to bird flu disinfectant prepared by the preparation method of embodiment 1-8:
It 100 times, is reacted at a temperature of 25 DEG C with the suspension of avian influenza virus and chicken manure respectively, thimerosal and avian influenza virus and chicken manure
Suspension volume ratio is 9:1, and counts the killing virus results of bird flu disinfectant effect different time, and experiment is repeated 3 times, as a result
Referring to table 3.
Killing effect (n=3) of the 3 bird flu disinfectant of table to bird flu
As can be seen from Table 3, thimerosal 1:50 times effect under, with avian influenza virus action time be 5min when, implement
Example 1-3, the viral killing rate of embodiment 7 are suitable, and there was no significant difference, and embodiment 4-6, the viral killing rate of embodiment 8 are significant
Higher than embodiment 1；With avian influenza virus action time be 10min when, embodiment 1-3, the viral killing rate of embodiment 7 are suitable,
There was no significant difference, embodiment 4-6, embodiment 8 viral killing rate be significantly higher than embodiment 1, viral killing rate is 100%,
Illustrate that avian influenza virus is killed completely；With avian influenza virus action time be 20min when, the viral killing rate of embodiment 1-8
Quite, there was no significant difference, and viral killing rate is 100% at this time, illustrates 1:50 times of diluted bird flu disinfectant energy in 20min
It is enough to kill avian influenza virus.
Thimerosal 1:100 times effect under, with avian influenza virus action time be 5min when, embodiment 1-3, embodiment 7
Viral killing rate it is suitable, there was no significant difference, embodiment 4-6, embodiment 8 viral killing rate be significantly higher than embodiment 1；With
When avian influenza virus action time is 10min, embodiment 1-3, the viral killing rate of embodiment 7 are suitable, and there was no significant difference, real
Apply a 4-6, the viral killing rate of embodiment 8 is significantly higher than embodiment 1；With avian influenza virus action time be 20min when, implement
The viral killing rate of example 1-8 is suitable, and there was no significant difference, and viral killing rate is 100% at this time, illustrate in 20min 1:100 times it is dilute
The bird flu disinfectant released can kill avian influenza virus.
Claims (7)
1. a kind of disinfectant for killing avian influenza virus, which is characterized in that raw material forms according to the following ratio: 30wt% peroxidating
Hydrogen 15-25 parts by volume, 20wt% Peracetic acid 2-6 parts by volume, sodium pyrophosphate 0.5-2.5 mass parts, 40wt% dodecyl two
Methylbenzyl ammonium bromide 1-3 parts by volume, sodium bicarbonate 1-3 mass parts, polyoxyethylene alkyl ether phosphate 1-4 mass parts, deoxidation
Sodium taurocholate 0.1-0.3 mass parts, nano zine oxide 2-4 mass parts, 75wt% ethyl alcohol 15-25 parts by volume, deionized water 80-100
Parts by volume, wherein 1 mass parts correspond to 1 parts by volume, 1g corresponds to 1mL.
2. killing the disinfectant of avian influenza virus according to claim 1, which is characterized in that the PH of the bird flu disinfectant
Value is 6.0-8.0.
3. killing the disinfectant of avian influenza virus according to claim 1, which is characterized in that the original of the bird flu disinfectant
Material further includes attapulgite clay powder 4-8 mass parts.
4. killing the disinfectant of avian influenza virus according to claim 1, which is characterized in that the original of the bird flu disinfectant
Material further includes wormwood essential oil 4-8 parts by volume.
5. killing the disinfectant of avian influenza virus according to claim 1, it is characterised in that the original of the bird flu disinfectant
Material further includes a nanometer Cu2Zn(PO4)(OH)3(H2O)2Powder 4-8 mass parts.
6. described in claim 1 kill avian influenza virus disinfectant preparation method, which is characterized in that in accordance with the following steps into
Row:
(1) it prepares organic liquor: according to parts by weight, deionized water 40-50 parts by volume being imported in mixing and stirring tank, temperature in tank
15-45 DEG C, 30wt% hydrogen peroxide 15-25 parts by volume is imported in blending tank and is mixed with deionized water, and stirs evenly and is made
Hydrogen peroxide, in sequence respectively by 75wt% ethyl alcohol 15-25 parts by volume, polyoxyethylene alkyl ether phosphate 1-4 mass parts,
40wt% dodecyl dimethyl benzyl ammonium bromide 1-3 parts by volume, 20wt% Peracetic acid 2-6 parts by volume import in blending tank,
Revolving speed 600r/min, is uniformly mixed that be configured to organic liquor spare；
(2) it prepares inorganic salt solution: according to parts by weight, deionized water 40-50 parts by volume being imported in mixing and stirring tank, in tank
It 15-45 DEG C of temperature, by sodium pyrophosphate 0.5-2.5 mass parts, nano zine oxide 2-4 mass parts, sodium bicarbonate 1-3 mass parts, takes off
Oxycholic acid sodium 0.1-0.3 mass parts are added in mixing and stirring tank, revolving speed 1000r/min, are uniformly mixed and are configured to inorganic salt solution
It is spare；
(3) by step (2) the inorganic salt solution steps for importing (1) organic liquor, revolving speed 600r/min is simultaneously uniformly mixed
Disinfectant semi-finished product are obtained, disinfectant semi-finished product are imported into high pressure homogenizer, then homogeneous 2-4 times, homogenization pressure 15-25MPa,
15-45 DEG C of matter temperature, obtains disinfectant for treating fowl flu.
7. killing the preparation method of the disinfectant of avian influenza virus according to claim 6, which is characterized in that the step
(2) attapulgite clay powder 4-8 mass parts or wormwood essential oil 4-8 parts by volume or nanometer Cu can also be added2Zn(PO4)(OH)3(H2O)2
Powder 4-8 mass parts.
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