CN110896930A - Method and simple device for raising coffee bean weevils - Google Patents
Method and simple device for raising coffee bean weevils Download PDFInfo
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- CN110896930A CN110896930A CN201911272354.0A CN201911272354A CN110896930A CN 110896930 A CN110896930 A CN 110896930A CN 201911272354 A CN201911272354 A CN 201911272354A CN 110896930 A CN110896930 A CN 110896930A
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- weevils
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- 230000019617 pupation Effects 0.000 claims 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to a method for rearing coffee weevils, which comprises a method for rearing adults and larvae of coffee weevils and feed therefor. The breeding method can stably obtain the insect states of coffee bean weevils with consistent ages, and is used for further scientific research work.
Description
Technical Field
The invention belongs to the technical field of insect breeding, and particularly relates to a breeding method of coffee bean weevils.
Background
Coffee bean elephant (De Geer) belongs to Coleoptera, Rhynchophyllaceae and is originated in India, has wide spread along with trade, is now a worldwide storage pest, and is particularly serious in tropical and subtropical regions. After a plurality of national grain storage pest surveys, the pest is listed as one of 5 grain storage pests with potential risks in China. According to the report, after the high-quality coffee beans stored for 1-3 years are bred into the imagoes for 7 days, the damage rate of the coffee beans reaches 31% -87%, and the inferior coffee beans are soft, easy to invade and higher in damage rate. After the coffee bean weevil is invaded, the weight loss of the damaged coffee bean can be 30% within 6 months. The insect can propagate and harm in the field and in the storehouse. The coffee bean weevil has wide eating habits, wherein hosts with serious harm comprise corns, coffee beans, potatoes, garlic, traditional Chinese medicinal materials and the like. It is also found to have certain harm to citrus, barbadosnut, papaya, neem and other plant fruits. In addition, coffee bean weevils have been recognized as one of the important pests harming distiller's yeast by many breweries in China, and the main harm is to influence the yield of the distiller's yeast, reduce the physicochemical indexes of yeast blocks and the like.
Therefore, the prevention and control of coffee bean weevils will attract more and more attention and will become a hot point of research. Scientific research needs a large amount of tested insects, field collection is very easily affected by seasons, labor cost is high, the age of the insects cannot be guaranteed, and the requirement of research cannot be met. In order to better develop the scientific research of the coffee weevils, the establishment of the standardized population of the coffee weevils in the laboratory is very necessary.
Disclosure of Invention
The invention aims to provide a laboratory breeding method of coffee bean weevils, which is simple and easy to implement, low in cost, regular in insect age and high in survival rate. The method comprises the raising of the imagoes, eggs and larvae of the coffee bean weevils, and specifically comprises the following steps:
adult insects are raised in an insect cage by adopting a feed cake containing corn, red date and yeast, and the feed cake is used for enabling the coffee bean weevil to lay eggs. Collecting feed cake with worm eggs, feeding in larva feeding barrel filled with corn flour until adult eclosion, and starting new feeding cycle.
The method for breeding the coffee weevils establishes a method for artificially breeding the coffee weevils in a laboratory, and is convenient for obtaining the coffee weevils with consistent ages in days and meeting the quantity required by experiments.
The method has the advantages that the coffee bean weevil is fed in a laboratory, and the establishment of a standardized feeding technology is beneficial to developing various indoor research works. The method has the advantages of wide raw material source, low cost and strong practicability; secondly, the method is simple and easy to operate, saves labor cost, and is easy to realize standardized feeding technology.
Detailed Description
To more clearly describe the feeding method of the present invention, the present invention will be described in further detail below.
All the insect state feeding conditions are consistent, and the insect room environment is preferably as follows: at 25-35 deg.c and relative humidity of 50-80%, and protecting from light.
(1) Adult rearing
A breeding container: adult insects are raised in an insect cage. The insect cage adopts a gauze or a stainless steel net; the meshes are about 120 meshes, and the coffee bean weevil can not escape; the skeleton of the insect cage can be made of wood, plastic or stainless steel; the size of the insect cage is larger than 30cm multiplied by 30 cm.
Adult feed: mixing corn flour and fructus Jujubae at a ratio of 10:1-3:1, adding yeast powder 2-5%, adding appropriate amount of water, crushing in soybean milk machine, mixing, and placing in 9cm culture medium. After fermentation and air drying, the round cake type feed with the thickness of about 1cm is obtained.
The feeding method comprises the following steps: spraying water on both sides of the feed cake, and placing in adult rearing cage.
(2) Collection of eggs
The feed cake with high red date content is used for enabling the coffee bean weevil to lay eggs, the feed cake is sprayed with water and then placed in a feeding cage, and the feed cake is taken out after 1 day of laying eggs. Placing in the insect room for about 5-7 days, and waiting for larva hatching.
(3) Larva rearing
Description of the drawings:
FIG. 1: coffee bean weevil larva rearing bucket, its main characterized in that: consists of an upper cover (1) with a big upper funnel and a small lower funnel and a lower feeding barrel (3) of Beijing Zhongjie Square Biotechnology GmbH; the two parts are connected together by a clamping groove (4) at the lower edge of the upper cover and a hoop (5) arranged outside the upper edge of the rearing barrel; the funnel is deeply inserted into a small opening in the barrel and is covered with gauze with the mesh size of less than 120 meshes to prevent the coffee bean weevils from entering and exiting. Corn meal of about 2cm depth was added to a larval rearing tank as shown in FIG. 1, then a feed cake with eggs was placed, and about 1 ml of water was sprayed to the top of the tank, and 3 days and 5 days later, 1 ml of water was sprayed. The larva breeding barrel is placed in a dark environment.
The larvae which are hatched just before are fed in the feed cake, and after being slightly larger, the larvae enter the corn flour at the bottom of the barrel to continue growing, and the larvae of the later age form pupa chambers in the corn flour and emerge through eclosion. And finally, transferring the eclosion coffee weevils to an insect cage, and starting new-generation breeding.
To verify the effectiveness of the feeding method, 3 sets of experiments were set, and 10 mated weevils, which had emerged for 10 days, were selected for each set. The breeding was continued for 3 generations according to the above breeding method. And (5) observing the biological result in the observation period, and recording the number of the first generation, the egg + larva + pupal period, the service life of the adult and the male-female ratio.
TABLE 1 biological results of coffee weevils
From the test results, the device and the breeding method have no significant difference in indexes such as the development period of the larvae, the service life of adults, the egg laying amount and the like among generations, and the fact that the breeding method can obtain standardized population of the coffee bean weevil laboratory is shown. Moreover, the method has the advantages of simple feeding, low cost, easy obtaining of the insect states of the coffee bean weevils which are consistent in age and pure, and realization of mass feeding only by increasing the number of containers.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.
Claims (5)
1. A method for breeding coffee bean weevil comprises breeding adult and larva of coffee bean weevil and its feed.
2. The adult feed as claimed in claim 1, wherein the adult feed is a feed cake prepared by mixing and fermenting corn, red date and yeast.
3. The adult feeding method according to claim 1, wherein the adult is bred in an insect breeding cage with the feed cake according to claim 2, and allowed to lay eggs in the feed cake.
4. The larva raising method according to claim 1, wherein the feed cake with the eggs of the coffee weevil is raised in a larva raising barrel filled with corn flour, and the larva hatched first feeds the feed cake, and then the corn flour is drilled in until pupation and emergence occur.
5. The preferable breeding environment of the coffee bean weevils is as follows: at 25-35 deg.c and relative humidity of 50-80%, and protecting from light.
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CN201911272354.0A CN110896930A (en) | 2019-12-12 | 2019-12-12 | Method and simple device for raising coffee bean weevils |
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CN201911272354.0A CN110896930A (en) | 2019-12-12 | 2019-12-12 | Method and simple device for raising coffee bean weevils |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100071096A1 (en) * | 2006-11-29 | 2010-03-18 | Mitsui Chemicals, Inc. | Plant disease and insect damage control composition and plant disease and insect damage prevention method |
CN105340835A (en) * | 2015-11-18 | 2016-02-24 | 广东省粮食科学研究所 | Powder-feeding storage test insect rapid breeding forage and breeding method |
CN107279136A (en) * | 2016-07-14 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of araecerus fasciculatus attractant and its application |
CN107432279A (en) * | 2016-05-27 | 2017-12-05 | 河北省农林科学院植物保护研究所 | Araecerus fasciculatus aggregation pheromone composition and its application |
-
2019
- 2019-12-12 CN CN201911272354.0A patent/CN110896930A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100071096A1 (en) * | 2006-11-29 | 2010-03-18 | Mitsui Chemicals, Inc. | Plant disease and insect damage control composition and plant disease and insect damage prevention method |
CN105340835A (en) * | 2015-11-18 | 2016-02-24 | 广东省粮食科学研究所 | Powder-feeding storage test insect rapid breeding forage and breeding method |
CN107432279A (en) * | 2016-05-27 | 2017-12-05 | 河北省农林科学院植物保护研究所 | Araecerus fasciculatus aggregation pheromone composition and its application |
CN107279136A (en) * | 2016-07-14 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of araecerus fasciculatus attractant and its application |
Non-Patent Citations (3)
Title |
---|
张炳炎: "《中国枣树病虫草害及其防控原色图谱》", 31 December 2016 * |
李灿: "检疫性害虫咖啡豆象在贵州的危害特点及其生活史", 《贵州农业科学》 * |
杨帅等: "相对湿度对咖啡豆象生长发育、繁殖及种群增长的影响", 《应用昆虫学报》 * |
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