CN110879292A - Blood physical and chemical analysis method - Google Patents
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- CN110879292A CN110879292A CN201911203591.1A CN201911203591A CN110879292A CN 110879292 A CN110879292 A CN 110879292A CN 201911203591 A CN201911203591 A CN 201911203591A CN 110879292 A CN110879292 A CN 110879292A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 126
- 239000008280 blood Substances 0.000 title claims abstract description 126
- 238000009614 chemical analysis method Methods 0.000 title description 5
- 238000004940 physical analysis method Methods 0.000 title description 5
- 238000001514 detection method Methods 0.000 claims abstract description 42
- 210000000601 blood cell Anatomy 0.000 claims abstract description 20
- 238000000518 rheometry Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000005064 physico chemical analysis method Methods 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 11
- 238000004458 analytical method Methods 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims description 14
- 230000010100 anticoagulation Effects 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 8
- 238000005259 measurement Methods 0.000 claims description 6
- 238000005070 sampling Methods 0.000 claims description 5
- 238000007664 blowing Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 2
- 210000002381 plasma Anatomy 0.000 abstract description 42
- 238000012360 testing method Methods 0.000 abstract description 14
- 210000004180 plasmocyte Anatomy 0.000 abstract description 3
- 238000004159 blood analysis Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 238000008789 Direct Bilirubin Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 102000009027 Albumins Human genes 0.000 description 4
- 108010088751 Albumins Proteins 0.000 description 4
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 4
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- 229940116269 uric acid Drugs 0.000 description 4
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 3
- 108010082126 Alanine transaminase Proteins 0.000 description 3
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 3
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 238000010241 blood sampling Methods 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- 238000008050 Total Bilirubin Reagent Methods 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4915—Blood using flow cells
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- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
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- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention relates to a blood physicochemical analysis method, and belongs to the field of blood analysis. The method comprises the steps of firstly, enabling a whole blood sample to pass through a centrifugal separation device and a sample separation device to respectively form plasma and blood cells, detecting the plasma by using a blood biochemical detection device to obtain biochemical indexes of the whole blood sample, and detecting the plasma by using a blood rheology detection device to obtain the viscosity of the plasma of the whole blood sample; then, the plasma and the blood cells are mixed in equal amounts to form a whole blood sample again, and the viscosity of the whole blood is measured by a blood rheology measuring device. The blood physicochemical analysis method has the advantages of small sample amount, simple operation, short test time and short test process, and can reduce the problem of misplacing the sample caused by human errors, thereby improving the accuracy of the test result; in addition, the analysis method can simultaneously detect the indexes of the water biochemical index, the blood plasma viscosity and the whole blood viscosity, and has good application prospect.
Description
Technical Field
The invention belongs to the field of blood analysis, and particularly relates to a blood physicochemical analysis method.
Background
The physical and chemical analyzer is a medical clinical chemical detecting instrument integrating electronic, optical, computer and various biochemical analyzers. It has all functions of full-automatic biochemical apparatus and blood rheometer, can analyze biochemical indexes of ALT (alanine aminotransferase), AST (aspartate aminotransferase), ALP (alkaline phosphatase), GGT (gamma-glutamine transferase), T-Bil (total bilirubin), D-Bill (direct bilirubin), GLU (glucose), CRE (creatinine), UA (uric acid) and the like of blood, and can analyze rheological indexes of blood such as: whole blood viscosity, plasma viscosity, hematocrit, erythrocyte sedimentation rate, sedimentation equation K value and the like. The blood biochemical detection and the pressure type blood rheology detection are two detection modes for different types of indexes of blood; the two detection modes are not combined together and are not applied to a complete medical instrument in the market. Because the sample that blood biochemical detection device needs is plasma, and the sample that pressure type blood rheological device needs is plasma and whole blood, consequently the connection between the detection device and reasonable sample distribution scheme can practice thrift the sample, reduce the sample pollution, can reduce test time again, improve detection efficiency. In the conventional medical detection, 1ml of plasma is usually required to be used as a sample for detecting the biochemical indexes of blood, and at least 2ml of venous blood of a patient needs to be collected according to the blood separation ratio; the detection of the blood rheology index requires the collection of 5ml of venous blood from the patient, and then approximately 7ml of whole blood would be required if we performed each type of index separately. And for the detection of the conventional hemorheology, whole blood is usually firstly sucked from a test tube, is conveyed to a hemorheology detection device through a sampling needle to detect the viscosity of the whole blood, is then discharged into an anticoagulation tube, is kept stand for 1-2h to obtain plasma or is centrifuged by a centrifuge to obtain the plasma, and the viscosity of the plasma is detected. The conventional detection method is required to detect two indexes, the required sample amount is large, the operation is complex, the testing time is long, and human errors possibly exist even when the test tube is drawn out for centrifugation in the testing process, so that the position of the sample is misplaced, and the accuracy of the testing result is influenced.
Therefore, a new blood physicochemical analysis method needs to be proposed.
Disclosure of Invention
In view of the above, the present invention provides a method for physicochemical analysis of blood.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for physicochemical analysis of blood, said method comprising the steps of:
(1) centrifuging a whole blood sample in an anticoagulation tube by using a centrifugal machine, and then placing the sample in a sample tube, wherein the upper layer in the sample tube is plasma and the lower layer is blood cells;
(2) passing the sample tube through a sample separation device to obtain plasma, transporting the plasma to a blood biochemical detection device, and measuring to obtain biochemical indexes of the whole blood sample;
(3) continuously distinguishing the sample tubes through a liquid level detection device, enabling the obtained upper-layer plasma to enter a blood rheology detection device, and measuring the viscosity of the plasma of the whole blood sample;
(4) and mixing the plasma in the blood rheology detection device and the blood cells in the sample tube in an equal amount in a blood mixing device to form a whole blood sample, entering the blood rheology measurement device, and measuring the whole blood viscosity of the whole blood sample.
Preferably, the whole blood sample in step (1) is venous blood of a patient, and the volume of the anticoagulation tube is 5 ml.
Preferably, the rotating speed of the centrifuge in the step (1) is 2000rpm, and the centrifugal treatment is carried out for 5-10 min.
Preferably, the hemorheology detecting device in the step (3) is a pressure-type hemorheology detecting device.
Preferably, the blood cells in step (4) need to be mixed by a stirring device before being mixed with the blood plasma.
Preferably, the stirring device adopts an inclined paddle type stirring sheet combined with the sampling needle.
Preferably, the blood blending device in the step (4) is used for blending in a blowing and sucking mode.
The invention has the beneficial effects that:
the invention discloses a blood physical and chemical analysis method, which comprises the steps of firstly, enabling a whole blood sample to pass through a centrifugal separation device and a sample separation device to respectively form plasma and blood cells, utilizing a blood biochemical detection device to detect the plasma to obtain biochemical indexes of the whole blood sample, and utilizing a blood rheology detection device to detect the plasma to obtain the viscosity of the plasma of the whole blood sample; then, the plasma and the blood cells are mixed in equal amounts to form a whole blood sample again, and the viscosity of the whole blood is measured by a blood rheology measuring device. The blood physical and chemical analysis method integrates the blood biochemical detection and the blood rheological detection, reduces the sample dosage, reduces the manual operation time on the premise of ensuring the accuracy of the test result, simplifies the complexity of multi-department measurement, can relieve the pain of the patient in blood sampling for many times, and improves the detection efficiency.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
Example 1
A blood physicochemical analysis method comprises the following steps:
(1) taking venous blood of a patient with 5ml as a whole blood sample, taking a 5ml anticoagulation tube as a sample tube, placing the whole blood sample in the sample tube, centrifuging for 5min by using a centrifugal machine with the rotating speed of 2000rpm, and dividing the whole blood sample in the anticoagulation tube into plasma (2.6ml) positioned at the upper layer and blood cells (2.4ml) positioned at the lower layer;
(2) after the anticoagulation tube is continuously placed in a sample separation device, plasma (2.6ml) and blood cells (2.4ml) are respectively obtained, the plasma in the anticoagulation tube is transported to a blood biochemical detection device, and the biochemical indexes of the blood of the patient can be obtained by measuring (DB (direct bilirubin): 0.22umol/L, ALB (albumin): 38.3g/L, ALT (alanine aminotransferase): 54.1IU/L, AST (aspartate aminotransferase): 41.9IU/L, TG (triglyceride): 0.67nmol/L, UA (uric acid): 549.4umol/L, TP (total protein): 83.6 g/L);
(3) continuously distinguishing the sample tubes by a liquid level detection device, enabling the upper layer of plasma to enter a pressure type hemorheology detection device, and measuring to obtain the viscosity of the plasma of the patient (the plasma viscosity: 1.24 mPa.S);
(4) and (3) uniformly mixing the blood cells in the sample tube by using a slant paddle type stirring sheet combined with a sampling needle, uniformly mixing the blood cells with the plasma in the blood rheology detection device in the step (3) (uniformly mixing by adopting a blowing and sucking mode) to form whole blood again, feeding the whole blood into a blood rheology measurement device, and measuring to obtain the whole blood viscosity (the whole blood viscosity is 1/200S: 3.59mPa.S, the whole blood viscosity is 1/5S: 6.90mPa.S and the whole blood viscosity is 1/1S: 13.79) of the patient.
Example 2
A blood physicochemical analysis method comprises the following steps:
(1) taking venous blood of a patient with 5ml as a whole blood sample, taking a 5ml anticoagulation tube as a sample tube, placing the whole blood sample in the sample tube, centrifuging for 5min by using a centrifugal machine with the rotating speed of 2000rpm, and dividing the whole blood sample in the anticoagulation tube into plasma (2.6ml) positioned at the upper layer and blood cells (2.4ml) positioned at the lower layer;
(2) after the anticoagulation tube is continuously placed in a sample separation device, plasma (2.6ml) and blood cells (2.4ml) are respectively obtained, the plasma in the anticoagulation tube is transported to a blood biochemical detection device, and the biochemical indexes of the blood of the patient can be obtained by measuring (DB (direct bilirubin): 3.88umol/L, ALB (albumin): 50.5g/L, ALT (alanine aminotransferase): 13.2IU/L, AST (aspartate aminotransferase): 20.0IU/L, TG (triglyceride): 0.92nmol/L, GLU (blood sugar): 5.89mmol/L, TP (total protein): 74.9 g/L);
(3) continuously distinguishing the sample tubes by a liquid level detection device, enabling the upper layer of plasma to enter a pressure type hemorheology detection device, and measuring to obtain the viscosity of the plasma of the patient (the plasma viscosity: 1.85 mPa.S);
(4) and (3) uniformly mixing the blood cells in the sample tube by using a slant paddle type stirring sheet combined with the sampling needle, uniformly mixing the blood cells with the plasma in the blood rheology detection device in the step (3) (uniformly mixing by adopting a blowing and sucking mode) to form whole blood again, feeding the whole blood into a blood rheology measurement device, and measuring to obtain the whole blood viscosity (the whole blood viscosity is 1/200S: 3.88mPa.S, the whole blood viscosity is 1/5S: 9.00mPa.S and the whole blood viscosity is 1/1S: 20.39) of the patient.
The method comprises the steps of firstly, enabling a whole blood sample to pass through a centrifugal separation device and a sample separation device to respectively form plasma and blood cells, detecting the plasma by using a blood biochemical detection device to obtain biochemical indexes of the whole blood sample, and detecting the plasma by using a blood rheology detection device to obtain the viscosity of the plasma of the whole blood sample; then, the plasma and the blood cells are mixed in equal amounts to form a whole blood sample again, and the viscosity of the whole blood is measured by a blood rheology measuring device. The blood physicochemical analysis method has the advantages of small sample amount, simple operation, short test time and short test process, and can reduce the problem of misplacing the sample caused by human errors, thereby improving the accuracy of the test result; in addition, the analysis method can simultaneously detect the indexes of the water biochemical index, the blood plasma viscosity and the whole blood viscosity, and has good application prospect.
The blood physical and chemical analysis method integrates the blood biochemical detection and the blood rheological detection, reduces the sample dosage, reduces the manual operation time on the premise of ensuring the accuracy of the test result, simplifies the complexity of multi-department measurement, can relieve the pain of the patient in blood sampling for many times, and improves the detection efficiency.
Finally, the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the claims of the present invention.
Claims (7)
1. A method for physicochemical analysis of blood, said method comprising the steps of:
(1) centrifuging a whole blood sample in an anticoagulation tube by using a centrifugal machine, and then placing the sample in a sample tube, wherein the upper layer in the sample tube is plasma and the lower layer is blood cells;
(2) passing the sample tube through a sample separation device to obtain plasma, transporting the plasma to a blood biochemical detection device, and measuring to obtain biochemical indexes of the whole blood sample;
(3) continuously distinguishing the sample tubes through a liquid level detection device, enabling the obtained upper-layer plasma to enter a blood rheology detection device, and measuring the viscosity of the whole blood sample plasma;
(4) and mixing the plasma in the blood rheology detection device and the blood cells in the sample tube in an equal amount in a blood mixing device to form a whole blood sample, and feeding the whole blood sample into a blood rheology measurement device to measure the viscosity of the whole blood.
2. A method for physicochemical analysis of blood according to claim 1, wherein the whole blood sample in step (1) is venous blood of a patient, and the volume of the anticoagulation tube is 5 ml.
3. A physicochemical analysis method for blood as defined in claim 1, wherein the rotation speed of said centrifuge in step (1) is 2000rpm, and the centrifugation is performed for 5-10 min.
4. A physicochemical analysis method for blood according to claim 1, wherein in step (3), the hemorheology detection device is a pressure-type hemorheology detection device.
5. A physicochemical analysis method for blood as set forth in claim 1, wherein the blood cells in step (4) are mixed by a stirring device before being mixed with the plasma.
6. A method for physicochemical analysis of blood according to claim 5, wherein the stirring means employs a paddle-type stirring blade combined with a sampling needle.
7. A blood physicochemical analysis method according to claim 1, wherein the blood blending device in step (4) is used for blending in a blowing and sucking manner.
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| CN201911203591.1A CN110879292A (en) | 2019-11-29 | 2019-11-29 | Blood physical and chemical analysis method |
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| CN201911203591.1A CN110879292A (en) | 2019-11-29 | 2019-11-29 | Blood physical and chemical analysis method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101819124A (en) * | 2009-02-27 | 2010-09-01 | 北京普利生仪器有限公司 | Method for automatically measuring blood viscosity |
| CN201707339U (en) * | 2010-04-26 | 2011-01-12 | 常州鸿臻电子技术有限公司 | Full automatic testing center |
| CN102236009A (en) * | 2010-04-28 | 2011-11-09 | 上海安彩智能技术有限公司 | Integrated blood inspection platform and using method thereof |
| CN103499525A (en) * | 2013-09-03 | 2014-01-08 | 冯晓均 | Blood sample biochemical quantitative detection device |
| CN107966578A (en) * | 2017-12-26 | 2018-04-27 | 江苏柯伦迪医疗技术有限公司 | A kind of medical quick biochemical detection system and detection method |
-
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- 2019-11-29 CN CN201911203591.1A patent/CN110879292A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101819124A (en) * | 2009-02-27 | 2010-09-01 | 北京普利生仪器有限公司 | Method for automatically measuring blood viscosity |
| CN201707339U (en) * | 2010-04-26 | 2011-01-12 | 常州鸿臻电子技术有限公司 | Full automatic testing center |
| CN102236009A (en) * | 2010-04-28 | 2011-11-09 | 上海安彩智能技术有限公司 | Integrated blood inspection platform and using method thereof |
| CN103499525A (en) * | 2013-09-03 | 2014-01-08 | 冯晓均 | Blood sample biochemical quantitative detection device |
| CN107966578A (en) * | 2017-12-26 | 2018-04-27 | 江苏柯伦迪医疗技术有限公司 | A kind of medical quick biochemical detection system and detection method |
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Application publication date: 20200313 |
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