CN101819124A - Method for automatically measuring blood viscosity - Google Patents
Method for automatically measuring blood viscosity Download PDFInfo
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- CN101819124A CN101819124A CN200910078399A CN200910078399A CN101819124A CN 101819124 A CN101819124 A CN 101819124A CN 200910078399 A CN200910078399 A CN 200910078399A CN 200910078399 A CN200910078399 A CN 200910078399A CN 101819124 A CN101819124 A CN 101819124A
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Abstract
The invention relates to a method for automatically measuring blood viscosity, which comprises the following steps of: acquiring data by using a full-automatic blood rheometer, wherein the blood rheometer comprises a capillary viscosity measuring device, a rotary blood rheology measuring device and a blood mixing device; centrifuging a blood sample by using a centrifugal machine to completely separate blood cells from blood plasma; starting the capillary viscosity measuring device to measure the viscosity of a blood plasma sample; re-injecting the used blood plasma sample into a sample tube; uniformly mixing the blood samples into a whole-blood state; starting a sampling needle to suck the whole blood from the sample tube and injecting the whole blood into the rotary blood rheology measuring device, and measuring the viscosity of the whole blood; and processing the measured data by using a resolving processor to generate a measuring report. The method for automatically measuring the blood viscosity eliminates manual operation error, simplifies operation steps, and shortens test time.
Description
Technical field
The present invention relates to a kind of method of automatically measuring blood viscosity, this method uses hydro-extractor that the blood sample low-speed centrifugal of gathering is handled, and uses the capillary viscosity measurement mechanism, measures plasma sample viscosity; Again used plasma sample is recycled in the sample tube, blood sample is mixed into the whole blood state, from sample tube, draw whole blood, use rotary hemorheology measurement mechanism to measure whole blood viscosity.
Background technology
Chinese patent 200520103130.4 discloses the method for measuring whole blood viscosity and plasma viscosity, and in medical field tests, the hemorheology instrument is mainly used in test whole blood viscosity and plasma viscosity.Whole blood viscosity belongs to non-Newtonian fluid, and the comparison reasonable method that adopts is rotary process usually, and the equipment of use has full-automatic hemorheology instrument.Blood plasma belongs to Newtonian fluid, can use rotary process, also can test its viscosity with capillary tube technique, by comparison, tests plasma viscosity with capillary tube technique, and blood using amount is less, and precision is higher, saves time; Normally used instrument has capillary viscosimeter.Because full-automatic hemorheology instrument and capillary viscosimeter are two kinds of instruments, two kinds of method of testings; Also not with these two kinds of method of testings, be received into an instrument on the instrument on the market.Therefore, in practical operation, often just formed or use capillary viscosity instrumentation whole blood viscosity also to survey plasma viscosity; Survey whole blood viscosity and plasma viscosity with rotary full-automatic hemorheology instrument; Though these two kinds of testing tools can be used, and are not the most rational selections; Optimal method of testing is to survey the whole blood viscosity rotary process; Survey the plasma viscosity capillary tube technique.So both convenient, accurate again, save blood again.In medical field tests, conventional way is to extract the about 5ml of patient's blood sample earlier, then with in the blood sample injecting tube, patient's numbering is marked on the test tube; At first from test tube, draw the part whole blood, inject testing tool, measure whole blood viscosity; The test tube that secondly the residue whole blood will be housed takes out from the instrumentation tube position, puts into the horizontal hydro-extractor, makes haemocyte and separating plasma; Blood plasma injects testing tool, measures plasma viscosity; The measurement result that instrument obtains from each pipe position generates measures report.There is big defective in conventional method of testing, because in the measuring process, plug test tube repeatedly, change a patient's pipe item, not only can cause the prolongation of test period, also can be because of artificial negligence of operation, and cause misplacing the pipe position, perhaps set wrong pipe item, thereby influence precision of test result.Therefore, a kind of method of new automatically measuring blood viscosity need to be proposed.
Summary of the invention
The object of the present invention is to provide a kind of method of automatically measuring blood viscosity, this method uses hydro-extractor that the blood sample low-speed centrifugal of gathering is handled, and uses the capillary viscosity measurement mechanism, measures plasma sample viscosity; Again used plasma sample is recycled in the sample tube, blood sample is mixed into the whole blood state, from sample tube, draw whole blood, use rotary hemorheology measurement mechanism to measure whole blood viscosity.
The objective of the invention is to realize by following technical proposals: a kind of method of automatically measuring blood viscosity, use a fully automatic blood flow graph to carry out data acquisition, utilization is resolved processor data is handled, and exports the viscosity data of tested blood; Described hemorheology instrument comprises capillary viscosity measurement mechanism, rotary hemorheology measurement mechanism, blood mixing arrangement;
A, the anti-freezing of collection patient venous samples can are handled in the injecting sample pipe, and this sample tube mark is handled;
B, described sample tube is inserted hydro-extractor to described blood sample centrifugal treating, haemocyte is separated fully with blood plasma;
Sample tube after c, the centrifugal treating is put into the testing tube position of described hemorheology instrument, sets the testing tube item of described sample tube, starts described capillary viscosity measurement mechanism, extracts the plasma sample in the described sample tube, measures described plasma sample viscosity; Again used plasma sample is recycled in the sample tube;
Blood mixing arrangement in d, the described hemorheology instrument of startup, blood sample in the described sample tube is carried out mixing, blood sample is mixed into the whole blood state, start sample needle and from sample tube, draw whole blood, inject described rotary hemorheology measurement mechanism, measure described whole blood sample viscosity;
E, utilization are resolved processor the data of being measured are handled, and generate to measure report.
Compared with the prior art the present invention has following advantage:
1, the present invention measures traditional blood viscosity needs twice intubate (refering in particular to sample tube) to make intubate into one time, and former testing tube position-finding need not to reset the testing tube item, has stopped the manually-operated error, has also simplified operation steps, has shortened test period.
2, the present invention adopts the low-speed centrifugal separating treatment to blood sample, haemocyte can be separated fully with blood plasma, and haemocyte is sustained damage because of high speed centrifugation causes supercompression.
3, the present invention adopts former testing tube position oar formula to stir, and this alr mode can not cause damage to haemocyte.
4, the present invention adopts centrifuging, measures plasma viscosity earlier, reclaims plasma sample, measures whole blood viscosity behind the mixing again, has reduced the collection capacity and the use amount of blood sample.
5, the present invention has changed traditional mensuration order, reduced by two operation stepss, full-automatic whole blood and plasma viscosity for great amount of samples are measured, and the workload that has promptly reduced operating personnel has also shortened the test duration greatly, have improved the reliability of testing result simultaneously.
Description of drawings
The invention will be further described below in conjunction with drawings and Examples.
Fig. 1 is an equipment synoptic diagram involved in the present invention.
Embodiment
Embodiment one:
A kind of method of automatically measuring blood viscosity uses a fully automatic blood flow graph to carry out data acquisition, and utilization is resolved processor data are handled, and exports the viscosity data of tested blood; Described hemorheology instrument comprises capillary viscosity measurement mechanism, rotary hemorheology measurement mechanism, blood mixing arrangement, computer installation; The used fully automatic blood flow graph of present embodiment can also can adopt the LBY-N6C fully automatic blood flow graph of selling on the market with reference to China Patent No. 200510092821.3 disclosed measurement mechanisms.
A, the anti-freezing of collection patient venous samples can are handled in the injecting sample pipe, and this sample tube mark is handled; Normally from the about 5ml of vein venous blood samples, put into anti-coagulants in the sample tube in advance, shake up anti-freezing then, patient's numbering is marked on the sample tube; Sample tube also can be called test tube;
B, use hydro-extractor make haemocyte separate fully with blood plasma to described blood sample centrifugal treating;
In the present embodiment, used hydro-extractor is LDZ4-1.2 low speed autobalancing centrifuge (the upright hydro-extractor company limited in capital, Beijing produces), and the rotating speed of described hydro-extractor is 800-2000 rev/min, centrifugal treating 3-7 minute; The rotating speed of centrifugal treating the best is 1600 rev/mins, centrifugal treating 3 minutes; Such processing both can obtain pure blood plasma, and haemocyte was not caused damage, for use in measuring whole blood viscosity behind the mixing again; In the present embodiment, also can adopt the index of control centrifugal force to realize that haemocyte and blood plasma are intact and separate, the centrifugal force that blood sample is applied is 64g-484g, and centrifugal treating 3-7 minute, best centrifugal force was 256g, centrifugal treating 3 minutes.The present invention also can adopt LDI4-0.8 horizontal hydro-extractor (Beijing Medical Centrifugal Machine Factory), and perhaps other centrifugal treating mode is not described one by one at this;
Sample tube after c, the centrifugal treating is put into the testing tube position of described hemorheology instrument, sets the testing tube item of described sample tube, starts the capillary viscosity measurement mechanism, extracts the plasma sample in the described sample tube, measures described plasma sample viscosity; Again used plasma sample is recycled in the sample tube; Used capillary viscosity measurement mechanism is an application of sample capillary pipeline fluid viscosity measurement mechanism in the present embodiment, and the testing tube item of described setting sample tube is meant the residing coordinate position of above-mentioned sample tube, numbering, patient information input computing machine; The present invention also can adopt the capillary viscosimeter of other model, does not describe one by one at this;
Blood mixing arrangement in d, the described hemorheology instrument of startup, blood sample in the described sample tube is carried out mixing, blood sample is mixed into the whole blood state, start sample needle and from sample tube, draw whole blood, inject described rotary hemorheology measurement mechanism, measure described whole blood sample viscosity; In the present embodiment, described rotary hemorheology measurement mechanism belongs to conventional products; Described blood mixing arrangement can be an arm stirrer, also can be the hybrid mixing arrangements of vibrations, can also be suction jet flow mixing arrangement, can also be to shake to load in mixture to put.
Referring to Fig. 1, in the present embodiment, blood mixing arrangement 3 is arm stirrers, and the blade 31 of arm stirrer extend into to rotate in the sample tube to stir and just the haemocyte and the blood plasma of separated attitude can be mixed into the whole blood state.Comprise sample needle in the application of sample capillary pipeline fluid viscosity measurement mechanism in the present embodiment;
E, utilization are resolved processor the data of being measured are handled, and generate to measure report.
In the present embodiment, all finish to the operation of step e by computer control from step c.Referring to Fig. 1, comprise capillary viscosity measurement mechanism 1, sample needle 11 rotary hemorheology measurement mechanisms 2, blood mixing arrangement 3, blade 31, computer installation in the fully automatic blood flow graph; Can also be with reference to China Patent No. 200510092821.3 patent specifications.In the present embodiment, shaking hybrid mixing arrangement is the equipment that is made haemocyte and blood plasma mixing by mechanical shock; Suction jet flow mixing arrangement is to be aspirated repeatedly, returned and tell and make the equipment of haemocyte and blood plasma mixing by sample needle; Shake and load in mixture that to put be to rock the equipment that makes haemocyte and blood plasma mixing by clamper clamping sample tube.These three kinds of mixing arrangements all belong to known technology, are not described in detail.
In the present embodiment, the mensuration of plasma sample viscosity, the mensuration of whole blood viscosity is handled the data of being measured and all to be belonged to the proven technique content, is not described in detail at this.
Embodiment two:
Present embodiment is the improvement on embodiment one basis, and in the present embodiment, gathering patient's venous samples can volume is 3ml.The rotating speed of described centrifugal treating is 1600 rev/mins, centrifugal treating 4 minutes.Described blood mixing arrangement is an arm stirrer.Because the present invention measures plasma viscosity earlier, reclaim plasma sample, measure whole blood viscosity behind the mixing again, reduced the collection capacity and the use amount of blood sample, can reduce to 3ml by traditional blood sampling volume 5ml.The blood sample volume is the loss and misery that the collection of 3ml can alleviate the patient, reduces the treatment capacity of medical waste.
Claims (5)
1. the method for an automatically measuring blood viscosity is characterized in that: use a fully automatic blood flow graph to carry out data acquisition, utilize and resolve processor data are handled, export the viscosity data of tested blood; Described hemorheology instrument comprises capillary viscosity measurement mechanism, rotary hemorheology measurement mechanism, blood mixing arrangement;
A, the anti-freezing of collection patient venous samples can are handled in the injecting sample pipe, and this sample tube mark is handled;
B, described sample tube is inserted hydro-extractor to described blood sample centrifugal treating, haemocyte is separated fully with blood plasma;
Sample tube after c, the centrifugal treating is put into the testing tube position of described hemorheology instrument, sets the testing tube item of described sample tube, starts described capillary viscosity measurement mechanism, extracts the plasma sample in the described sample tube, measures described plasma sample viscosity; Again used plasma sample is recycled in the sample tube;
Blood mixing arrangement in d, the described hemorheology instrument of startup, blood sample in the described sample tube is carried out mixing, blood sample is mixed into the whole blood state, start sample needle and from sample tube, draw whole blood, inject described rotary hemorheology measurement mechanism, measure described whole blood sample viscosity;
E, utilization are resolved processor the data of being measured are handled, and generate to measure report.
2. the method for mensuration blood viscosity according to claim 1 is characterized in that: the centrifuge speed in the described blood sample centrifugal treating step is 800-2200 rev/min, centrifugal treating 3-7 minute.
3. the method for mensuration blood viscosity according to claim 1 is characterized in that: the centrifugal force that applies in the described blood sample centrifugal treating step is 64g-484g, centrifugal treating 3-7 minute.
4. the method for mensuration blood viscosity according to claim 1 is characterized in that: described mixing arrangement is an arm stirrer.
5. the method for mensuration blood viscosity according to claim 1 is characterized in that: described collection patient venous samples can volume is 3ml.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101983729A (en) * | 2010-11-17 | 2011-03-09 | 惠州市华阳多媒体电子有限公司 | Negative pressure wound therapy system capable of detecting viscosity of wound exudates |
| CN102175573A (en) * | 2010-12-21 | 2011-09-07 | 北京赛科希德科技发展有限公司 | Blood plasma detection method and system |
| CN108463708A (en) * | 2015-11-06 | 2018-08-28 | 密歇根大学董事会 | Microfluid rheometer system based on droplet |
| CN109932284A (en) * | 2019-03-15 | 2019-06-25 | 上海市嘉定区中心医院 | A kind of hemorheology batch detector methods of optimization |
| CN110879292A (en) * | 2019-11-29 | 2020-03-13 | 重庆大学 | Blood physical and chemical analysis method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003279460A (en) * | 2002-03-26 | 2003-10-02 | Kaname Wakasugi | Rheology measuring method for liquid and its device |
| CN2840922Y (en) * | 2005-08-01 | 2006-11-29 | 卢新 | Fully-automatic blood rheological instrument |
| CN1920526B (en) * | 2005-08-22 | 2012-01-11 | 北京普利生仪器有限公司 | Method and equipment for measuring fluid viscosity of capillary pipeline |
-
2009
- 2009-02-27 CN CN2009100783994A patent/CN101819124B/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101983729A (en) * | 2010-11-17 | 2011-03-09 | 惠州市华阳多媒体电子有限公司 | Negative pressure wound therapy system capable of detecting viscosity of wound exudates |
| CN101983729B (en) * | 2010-11-17 | 2013-05-15 | 惠州市华阳医疗电子有限公司 | Negative pressure wound therapy system capable of detecting viscosity of wound exudates |
| CN102175573A (en) * | 2010-12-21 | 2011-09-07 | 北京赛科希德科技发展有限公司 | Blood plasma detection method and system |
| CN102175573B (en) * | 2010-12-21 | 2013-01-16 | 北京赛科希德科技发展有限公司 | Blood plasma detection method and system |
| CN108463708A (en) * | 2015-11-06 | 2018-08-28 | 密歇根大学董事会 | Microfluid rheometer system based on droplet |
| US10845284B2 (en) | 2015-11-06 | 2020-11-24 | Regents Of The University Of Michigan | Droplet-based microfluidic rheometer system |
| CN108463708B (en) * | 2015-11-06 | 2021-10-08 | 密歇根大学董事会 | Micro-droplet-based microfluidic rheometer system |
| US11879820B2 (en) | 2015-11-06 | 2024-01-23 | Regents Of The University Of Michigan | Droplet-based microfluidic rheometer system |
| CN109932284A (en) * | 2019-03-15 | 2019-06-25 | 上海市嘉定区中心医院 | A kind of hemorheology batch detector methods of optimization |
| CN110879292A (en) * | 2019-11-29 | 2020-03-13 | 重庆大学 | Blood physical and chemical analysis method |
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| CN101819124B (en) | 2011-07-20 |
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Address after: 102206 first, second, fourth and fifth floors of building 3, No. 18, kekeyuan Road, Life Science Park, Changping District, Beijing Patentee after: Beijing Mindray Medical Instrument Co.,Ltd. Address before: 100085 Beijing city Haidian District Qunying Science Park Building 5, two floor East Patentee before: BEIJING PRECIL INSTRUMENT Co.,Ltd. |
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