CN204028091U - A kind of novel Platelet function Analyzer of multiparameter fast - Google Patents
A kind of novel Platelet function Analyzer of multiparameter fast Download PDFInfo
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- CN204028091U CN204028091U CN201420434208.XU CN201420434208U CN204028091U CN 204028091 U CN204028091 U CN 204028091U CN 201420434208 U CN201420434208 U CN 201420434208U CN 204028091 U CN204028091 U CN 204028091U
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Abstract
The utility model discloses a kind of novel Platelet function Analyzer of multiparameter fast.Instrument comprises porous connection cup, porous connection cup microscope carrier, diluted cup, detection cup, suction needle, suction needle mobile device, dilution syringe, sample injector, valve, pneumatic pump, waste drains pump and the main functional parts such as pick-up unit and control device and forms.Described analyser is behind each hole by same blood aliquot being put into respectively a Special multi-hole connection cup, the porous connection cup this being equipped with blood sample is placed on the instrument of the utility model design, by instrument to blood sample platelet counts and volume in each cup aperture of blood sample count detection in this porous connection each hole of cup, finally blood sample platelet counts in control wells blood sample platelet counts and other each hole is compared calculating, thus obtain respectively the aggregation rate of this blood sample under different induced polymerization inhibitor effect.
Description
Technical field
The utility model relates to a kind of novel Platelet function Analyzer of multiparameter fast, is a kind of novel Platelet function Analyzer device that can realize carrying out platelet function in blood sample multiparameter detection fast.
Background technology
Blood platelet is a kind of cell class particle composition in blood, is hemostasis and thrombotic key factor.Carrying out fast and easy detection to platelet function parameter, is an importance of platelet function research.Along with technical progress and going deep into blood platelet research, various countries have developed some instruments and methods to platelet function assay.As now existing: optics turbidimetry, electrode resistance method, thrombelastogram method, Flow cytometry etc.These methods or due to complex operation, blood using amount is large, testing result is not good, somewhat expensive, detection speed are slow etc., and reason can not adapt to correlative study needs very well.
The more platelet function assay instruments and methods of current use is photoelectric turbidimetry.But the method exists significantly not enough.Its operating process is: the whole blood of collection is first carried out low-speed centrifugal, precipitation red blood cell, leucocyte wherein, supernatant after centrifugal is collected as platelet rich plasma, then will wherein be transferred in another centrifuge tube by a part of platelet rich plasma again, carrying out high speed centrifugation makes blood platelet wherein also precipitate down, collect supernatant blood plasma (i.e. platelet poor plasma), then platelet counts in platelet rich plasma is adjusted, then first detect platelet poor plasma at special one than on turbid instrument, using this sample to instrument zeroing (as blank value), then detect platelet rich plasma (as blood platelet to light signal to obtained the maximum absorption), reduction (change) curve that induced polymerization inhibitor detects platelet rich plasma absorbance value after adding induced polymerization inhibitor is added after detection, reduce according to absorbance value and evaluate hematoblastic aggregation capability.The method, due to its blood using amount comparatively large (generally each test needs 2ml whole blood), operates the defects such as very loaded down with trivial details, efficiency is low, testing result less stable.Therefore, current platelet function assay instrument, method can't adapt to corresponding scientific research and clinical position needs very well.
Utility model content
Technical problem to be solved in the utility model is for the deficiencies in the prior art, provides a kind of Platelet function Analyzer and detection method thereof of novel multiparameter fast.
The technical solution realizing the utility model object is:
The utility model provides a kind of novel Platelet function Analyzer of multiparameter fast, and described analyser comprises suction needle mobile device, porous connection cup microscope carrier, dilution and pick-up unit;
Porous connection cup microscope carrier comprises the porous connection cup in loading stage and loading stage, and porous connection cup is formed by the tube combinations of multiple (more than one) cuvette cartridge, and its inside is isolated from each other, and outside is connected to an entirety.
Dilution and pick-up unit comprise diluted cup, detect cup and pick-up unit, and diluted cup and detection cup are arranged side by side, and arrange pick-up unit at detection cup sidewall, and described pick-up unit is for detecting the quantity in blood sample to be measured in blood platelet.
Suction needle mobile device comprises suction needle and drives the device of suction needle movement, suction needle mobile device is for drawing the blood sample in porous connection cup, transfer them in the diluted cup in dilution and pick-up unit, and in diluted cup, draw the blood sample after dilution, then transfer them to and detect in cup;
Wherein, porous connection cup microscope carrier is arranged side by side with dilution and pick-up unit, and suction needle mobile device is arranged on above dilution and pick-up unit.
Described analyser also comprises the first syringe, the second syringe, dilution memory storage, pneumatic pump and waste drains pump;
Wherein, suction needle connects the first syringe by pipeline, realizes drawing blood sample and distributing blood sample by the suction of the first syringe 10 and punching; Dilution memory storage connects diluted cup respectively by pipeline and detects cup, pipeline leads to rim of a cup, and valve is set at rim of a cup inlet end, the second syringe is also provided with between dilution memory storage and the pipeline of two glasss, and between dilution memory storage and the second syringe, valve is set, for injecting dilution in two glasss.
Its course of work is as follows:
Open the valve between dilution memory storage and the second syringe, second syringe extracts dilution in syringe pipeline, closes this valve, then opens the valve between the second syringe and diluted cup connecting pipe, promote the second syringe, then the dilution in the second syringe injects diluted cup; If but the valve now opened between the second syringe and analyzer cup connecting pipe, promote the second syringe, then the dilution in the second syringe injects analyzer cup.Cooperation between the driving process of the second syringe and each valve is closed and is opened, and realizes by additional motor.
Waste drains pump connects diluted cup respectively by pipeline and detects at the bottom of cup cup, and diluted cup, detection cup arrange valve at cup bottom outlet end respectively, and waste drains pump is connected with this pipeline, for the waste liquid in emptying cup; Between waste drains pump with two glasss of pipelines be connected, being also provided with pneumatic pump, and valve is set at the endpiece of pneumatic pump, for injecting air bottom diluted cup and detection cup, making blood sample and dilution fully dilute and mix.
Its course of work is as follows:
Open outlet of air pump end valve door, open diluted cup at the same time or separately and detect the valve on cup cup bottom outlet pipeline, pneumatic pump work can continuous delivery air by the pipeline bottom cup in two glasss or in the open cup of valve, make blood sample and dilution fully dilute and mix; Close the valve of pneumatic pump and outlet of air pump end, open diluted cup and detect cup cup bottom outlet end valve door, after waste drains pump startup work attracts the detection in two glasss, liquid is discharged outside instrument by pipeline under the driving of waste drains pump.Cooperation between above-mentioned valve is closed and is opened, and the control of waste drains pump and pneumatic pump is by equipment circuit and software simulating.
In the utility model, described suction needle mobile device samples for driving suction needle to join between cup, diluted cup, detection cup in porous, the movement locus of suction needle is rectilinear motion in the horizontal direction, namely above each cup of porous connection cup, diluted cup, detection cup position, does rectilinear movement sampling, distributes blood sample.
Specific works process is by realizing with under type:
Described suction needle mobile device comprises suction needle, moves up and down unit, horizontal movement unit, move up and down unit and comprise two pulleys setting up and down, and the travelling belt between pulley, between travelling belt, girt is set, pull bar one end connects suction needle, two pulley driving travelling belts rotate, thus drive girt vertical direction to move, and the final suction needle vertical direction that drives moves.Horizontal movement unit comprises horizontally disposed two pulleys, and the travelling belt between pulley, travelling belt with move up and down unit and be connected, two pulley driving travelling belts move reciprocatingly in the horizontal direction, thus drive and move up and down unit horizontal direction and move reciprocatingly, the final suction needle that drives moves reciprocatingly in the horizontal direction.Above-mentioned travelling belt moves up and down or moves horizontally and all realizes by additional motor.
In the utility model, described porous connection cup microscope carrier for loading porous connection cup, and mixes the blood sample to be measured in porous connection cup, stirs and/or heating and thermal insulation.
The utility model provides a kind of implementation to be: porous connection cup microscope carrier comprises porous connection cup, two pulleys of loading stage, loading stage inside, and the travelling belt between pulley; Wherein, two pulleys are setting up and down, and loading stage sidewall arranges top pulley, drive loading stage to rotate, connected between two pulleys by travelling belt.During work, drive motor drives below pulley rotation, thus the upper square wheel of drive rotates, and final drive before and after the porous connection cup in loading cup is rotated, and rotational angle is no more than 85 °, thus realizes the agitating function to blood sample in each cup.
The utility model provides another kind of implementation to be: in porous connection cup, add bar magnet in each cup, bottom each cup of loading stage, arrange stirring apparatus and magnet, thus realize the agitating function to blood sample in each cup.
The loading stage inside of above-mentioned two kinds of structures is also provided with heating temperature control device, for carrying out heated at constant temperature to the blood sample to be measured in porous connection cup.
In the utility model, described porous connection cup is the aggregate of the pipe line spread of 3-8 test tube shape, porous join the shape of each cup in cup and size identical, each cup upper internal diameter is 8-25mm, connection cup each cup endoporus clear depth is 15-100mm, and the bottom of each cup aperture is semisphere or inverted-triangular.
In the utility model, the movement locus of described suction needle is rectilinear motion in the horizontal direction, namely does rectilinear movement sampling along each cup of porous connection cup, diluted cup, detection cup position, distributes blood sample.
In the utility model, described porous connection cup microscope carrier has and join the cell texture or trench structure that cup-shaped matches with porous, join cup for safe placing porous.
The utility model also provides a kind of multiparameter Platelet function Analyzer to detect the method for platelet counts in blood sample, comprises the following steps:
Step 1, does not add any induced polymerization inhibitor reagent cup in contrast respectively, or load anti-coagulants in advance in this cup, and in other each cup, add the induced polymerization inhibitor of different cultivars or the induced polymerization inhibitor of same breed variable concentrations respectively in one of them cup of porous connection cup;
Step 2, by same blood sample respectively equivalent join in porous connection cup each cup, slight wobble mixing makes blood sample and reagent mix and isothermal reaction at 22-37 DEG C;
Step 3, joins cup and puts into porous connection cup microscope carrier, constantly stir and evenly mix through porous connection cup microscope carrier by porous;
Step 4, UNICOM first syringe and suction needle, draw in porous connection cup the blood sample to be measured contrasted in cup, add in diluted cup under the drive of suction needle mobile device by blood sample, UNICOM's pneumatic pump and diluted cup, inject air and make blood sample and cup dilution fully mix and dilute;
Step 5, then UNICOM crosses the first syringe and suction needle and quantitatively draws blood sample in diluted cup, transfer adds and detects in cup, UNICOM's pneumatic pump with detect cup, inject air and blood sample and cup dilution fully mixed and secondary dilution;
Step 6, the platelet counts of pick-up unit to blood sample in contrast cup, the volume that are arranged on detection cup sidewall detect and record;
Step 7, detects after terminating, instrument to diluted cup, detect cup, sampling probe and each pipeline and clean, UNICOM's waste drains pump and diluted cup, detect cup, emptying waste liquid; UNICOM's dilution memory storage and diluted cup, detect cup, savings dilution;
Step 8, in other cup of distich cup, blood sample repeats step 4 ~ step 7, completes respectively and carries out determination and analysis to other each cup platelet counts, volume in porous connection cup.
In the utility model, pick-up unit detects and record the platelet counts of blood sample in cup, the testing result of the platelet counts to each cup and contrast cup platelet counts calculated respectively according to following computing formula and obtain the platelet aggregation rate testing result of blood sample under various different induced polymerization inhibitor effect, computing formula is as follows:
In the utility model, pick-up unit detects and record the volume of platelets of blood sample in cup, utilize and the size of the average body product value not adding induced polymerization inhibitor thromboblast is judged whether blood sample is assembled before detection, thus judge whether blood sample is assembled before detection, to avoid detecting mistake; When not adding induced polymerization inhibitor thromboblast average external volume and being greater than more than 12.5fl, pick-up unit is reported to the police, and prompting may be assembled by blood sample, thus avoids carrying out the incorrect platelet aggregation rate result of detection acquisition to the blood sample that platelet aggregation occurs.
The utility model method and instrument also detect hematoblastic volume while detection platelet counts, and testing result represents with mean platelet volume (MPV).Instrument can by detecting the size not adding the hematoblastic average external volume of induced polymerization inhibitor blood sample (MPV) value, judge whether blood sample is assembled, its principle occurs obviously to strengthen (be greater than more than 20% of normal value maximal value or reach more than 12.5fl) if ought not add induced polymerization inhibitor blood sample volume of platelets, then illustrate that obvious gathering has occurred blood sample.The blood sample having occurred obviously to assemble probably will have larger difference with the aggregation rate value of normal blood sample, because utilizing in the utility model detection method, the size of the mean platelet volume not adding induced polymerization inhibitor blood sample (MPV) value is judged whether blood sample is assembled before detection, thus help operator to find the blood sample obviously occurring to assemble, avoid detecting mistake.The blood sample not adding induced polymerization inhibitor blood sample mean platelet volume (MPV) and be greater than more than 12.5fl is reported to the police by the utility model, and prompting may be assembled by blood sample.
The utility model method and instrument can also to not adding induced polymerization inhibitor sample, add the blood sample that induced polymerization inhibitor assembles platelet aggregation and respectively carry out twice or repeated detection, and use the mean value of mean value and each gathering pattern detection result not assembling the testing result of platelet counts to compare respectively to calculate and obtain the aggregation rate that each induced polymerization inhibitor (or variable concentrations induced polymerization inhibitor) acts on blood sample afterwards, the stability of testing result can be improved like this.
The pick-up unit of the utility model method and instrument can be arrange at detection wall of cup, also can be and detect an independent agency being separated of cup, by pipeline with detect cup be connected the sample after diluting transferred to independently in pick-up unit the platelet count and volume of platelets completed diluting blood sample detect.
Compared with prior art, its remarkable advantage is the utility model:
(1) the suction needle mobile device in the utility model analyser freely samples for driving suction needle, distributes blood sample, automation mechanized operation between each cup of porous connection cup, diluted cup, detection cup, and efficiency improves greatly.
(2) the porous connection cup microscope carrier in the utility model analyser has automatic mixing, automatic stirring and the function of heated at constant temperature.
(3) dilution in the utility model analyser and pick-up unit comprise diluted cup and detect cup for secondary dilution blood sample with carry out platelet counts and volume and detect.
(4) the first injection in the utility model analyser, the setting of the second syringe, dilution memory storage, pneumatic pump and waste drains pump, the sampling precision improving sample dilutes the vast scale of sample with achieving, and improves and detects the range of linearity of sample and the stability of testing result.
(5) the multi-joint cup in the utility model analyser is formed by the tube combinations of multiple test tube shape, its inside is isolated from each other, outside is connected to an entirety, its design is completely supporting with instrument, can realize carrying out multinomial induced polymerization inhibitor detection to a blood sample, efficiency is much higher than existing detection method simultaneously.
(6) the utility model multiparameter Platelet function Analyzer and the method for platelet counts in blood sample that detects have higher standardization and automatization level.
(7) described method can complete at every turn simultaneously and be no less than two kinds of induced polymerization inhibitor kinds to same blood examination or same breed is no less than the induced polymerization inhibitor of two kinds of variable concentrations to the detection of the sample of same blood sample treatments, namely can obtain the testing result of the platelet aggregation rate being no less than two kinds of disparity items simultaneously.
(8) described method can to not adding induced polymerization inhibitor sample, add the blood sample that induced polymerization inhibitor assembles platelet aggregation sample and respectively carry out twice or repeated detection, and use the mean value of the mean value and each gathering pattern detection result not assembling the testing result of platelet counts to compare to calculate the aggregation rate result obtaining blood sample after each induced polymerization inhibitor effect.
(9) the utility model method and Instrumental results can also, by providing original volume of platelets, help operator to judge whether blood sample is assembled before detection, thus can avoid detecting mistake.
(10) the utility model analyser detects the method for platelet counts and the coefficient R of turbidimetry Instrumental results in blood sample
2=0.8705, R=0.9330, correlativity is good, shows that the utility model method has higher feasibility.
Accompanying drawing explanation
Fig. 1 is the utility model analyser general structure schematic diagram (1-suction needle mobile device, 2-porous connection cup microscope carrier, 3-dilution and pick-up unit).
Fig. 2 is each parts connection diagram of the utility model analyser.
Fig. 3 is the utility model suction needle mobile device structural representation (9-suction needle, 15-moves up and down unit, and 16-moves left and right unit, 17-cross bar).
Fig. 4 is the utility model embodiment 3 porous connection cup microscope carrier front view (4-porous connection cup, 5-loading stage, pulley above 18-, pulley below 19-, 20-motor).
Fig. 5 is the utility model embodiment 3 porous connection cup microscope carrier side view (4-porous connection cup, 5-loading stage, pulley above 18-, pulley below 19-).
Fig. 6 is the utility model embodiment 4 porous connection cup microscope carrier front view (4-porous connection cup, 21-stirring apparatus, 22-heating temperature control device, 23-bar magnet, 24-magnet).
Fig. 7 is the utility model porous connection cup stereographic map.
To be the utility model suction needle do suction to the blood sample in porous connection cup to Fig. 8 fills blending manner schematic diagram.
Fig. 9 is the correlation analysis figure that the utility model analyser detects aggregation rate and the optics turbidimetry Instrument measuring maximum platelet aggregation rate obtained.
Embodiment
Embodiment 1
As illustrated in fig. 1 and 2, a kind of novel Platelet function Analyzer of multiparameter fast, described analyser comprises suction needle mobile device 1, porous connection cup microscope carrier 2, dilution and pick-up unit 3;
Porous connection cup microscope carrier 2 comprises the porous connection cup 4 in loading stage 5 and loading stage, and porous connection cup 4 is formed by the tube combinations of multiple test tube shape, and its inside is isolated from each other, and outside is connected to an entirety; Porous connection cup microscope carrier 2 has 4 equidistant lineal layout circular hole positions, and the porous connection cup that can be applicable to having accordingly multiple test tube shape cup is placed.
Dilution and pick-up unit 3 comprise diluted cup 6, detect cup 7 and pick-up unit 8, and diluted cup 6 and detection cup 7 are arranged side by side, and arrange pick-up unit 8 at detection cup 7 sidewall, and described pick-up unit 8 is for detecting the quantity in blood sample to be measured in blood platelet;
Suction needle mobile device 1 comprises suction needle 9 and drives the device of suction needle movement, suction needle mobile device is for drawing the blood sample in porous connection cup, transfer them in the diluted cup 6 in dilution and pick-up unit, and the blood sample drawn in diluted cup 6 after dilution, then transfer them to and detect in cup 7;
Wherein, porous connection cup microscope carrier 2 is arranged side by side with dilution and pick-up unit 3, and suction needle mobile device 1 is arranged on above dilution and pick-up unit 3.
Described analyser also comprises the first syringe 10, second syringe 11, dilution memory storage 12, pneumatic pump 13 and waste drains pump 14;
Wherein, suction needle 9 connects the first syringe 10 by pipeline, realizes drawing blood sample and distributing blood sample (as shown in Figure 7) by the suction of the first syringe 10 and punching; Dilution memory storage 12 connects diluted cup 6 respectively by pipeline and detects cup 7, pipeline leads to rim of a cup, and valve is set at rim of a cup inlet end, the second syringe 11 is also provided with between dilution memory storage 12 and the pipeline of two glasss, and between dilution memory storage 12 and the second syringe 11, valve is set, for injecting dilution in two glasss;
Waste drains pump 14 connects diluted cup 6 respectively by pipeline and detects at the bottom of 7 glasss, cup, and arranges valve at cup bottom outlet end, and waste drains pump 14 endpiece arranges valve, for the waste liquid in emptying cup; Between waste drains pump 14 and the pipeline of two glasss, being also provided with pneumatic pump 13, and valve is set at the endpiece of pneumatic pump 13, for injecting air bottom diluted cup 6 and detection cup 7, making blood sample and dilution again fully dilute and mix.
In porous described in the utility model connection cup the shape of each cup and size identical, be the aggregate of the pipe line spread of 3-8 test tube shape, each rim of a cup internal diameter is 8-25mm scope, and the endoporus clear depth joining cup is 15-100mm, and the bottom of each cup aperture is semisphere.
The movement locus of suction needle described in the utility model is rectilinear motion in the horizontal direction, namely does rectilinear movement sampling along each cup of porous connection cup, diluted cup, detection cup position, distributes blood sample.
Embodiment 2
Other structure is with embodiment 1, as shown in Figure 3, suction needle mobile device comprises suction needle 9, moves up and down unit 15, horizontal movement unit 16, move up and down unit 15 and comprise two pulleys setting up and down, and the travelling belt between pulley, girt 17 is set between travelling belt, pull bar one end connects suction needle 9, two pulley driving travelling belts rotate, thus drive girt 17 vertical direction to move, and final suction needle 9 vertical direction that drives moves; Horizontal movement unit 16 comprises horizontally disposed two pulleys, and the travelling belt between pulley, travelling belt with move up and down unit 15 and be connected, two pulley driving travelling belts move reciprocatingly in the horizontal direction, thus drive and move up and down unit 15 horizontal direction and move reciprocatingly, the final suction needle 9 that drives moves reciprocatingly in the horizontal direction.
In porous described in the utility model connection cup the shape of each cup and size identical, be the aggregate of the pipe line spread of 3-8 test tube shape, each rim of a cup internal diameter is 8-25mm scope, and the endoporus clear depth joining cup is 15-100mm, and the bottom of each cup aperture is semisphere.
Have an elongate aperture above porous connection cup microscope carrier 2 described in the utility model, the porous connection cup that can be applicable to having accordingly multiple test tube shape cup is placed.
The movement locus of suction needle described in the utility model is rectilinear motion in the horizontal direction, namely does rectilinear movement sampling along each cup of porous connection cup, diluted cup, detection cup position, distributes blood sample.
Embodiment 3
Other structure is with embodiment 1, and as shown in Figure 4, Figure 5, porous connection cup microscope carrier comprises porous connection cup 4, two pulleys of loading stage 5, loading stage 5 inside, and the travelling belt between pulley; Wherein, two pulleys are setting up and down, and loading stage sidewall arranges top pulley 18, and drive loading stage to rotate, below pulley 19 drives rotation by drive motor 20, is connected between two pulleys by travelling belt; During work, drive motor 20 drives below pulley 19 to rotate, thus drive top pulley 18 rotates, and finally drive the porous of loading in cup to join before and after cup 4 and rotate, rotational angle is no more than 85 °, thus the agitating function of realization to blood sample in each cup.
In porous described in the utility model connection cup the shape of each cup and size identical, be the aggregate of the pipe line spread of 3-8 test tube shape, each rim of a cup internal diameter is 8-25mm scope, and the endoporus clear depth joining cup is 15-100mm, and the bottom of each cup aperture is semisphere.
The movement locus of suction needle described in the utility model is rectilinear motion in the horizontal direction, namely does rectilinear movement sampling along each cup of porous connection cup, diluted cup, detection cup position, distributes blood sample.
Embodiment 4
Other structure, with embodiment 1, as shown in Figure 6, adds bar magnet 23 in each cup, bottom each cup of loading stage 5, arranges stirring apparatus 21 and magnet 24, thus realize the agitating function to blood sample in each cup in porous connection cup.Loading stage 5 inside is provided with heating temperature control device 22.
In porous described in the utility model connection cup the shape of each cup and size identical, be the aggregate of the pipe line spread of 3-8 test tube shape, each rim of a cup internal diameter is 8-25mm scope, and the endoporus clear depth joining cup is 15-100mm, and the bottom of each cup aperture is semisphere.
The movement locus of suction needle described in the utility model is rectilinear motion in the horizontal direction, namely does rectilinear movement sampling along each cup of porous connection cup, diluted cup, detection cup position, distributes blood sample.
Embodiment 5
Other structure is with embodiment 2, and as shown in Figure 4, Figure 5, porous connection cup microscope carrier comprises porous connection cup 4, two pulleys of loading stage 5, loading stage 5 inside, and the travelling belt between pulley; Wherein, two pulleys are setting up and down, and loading stage sidewall arranges top pulley 18, and drive loading stage to rotate, below pulley 19 drives rotation by drive motor 20, is connected between two pulleys by travelling belt; During work, drive motor 20 drives below pulley 19 to rotate, thus drive top pulley 18 rotates, and finally drive the porous of loading in cup to join before and after cup 4 and rotate, rotational angle is no more than 85 °, thus the agitating function of realization to blood sample in each cup.Loading stage 5 inside is provided with heating temperature control device 22.
In porous described in the utility model connection cup the shape of each cup and size identical, be the aggregate of the pipe line spread of 3-8 test tube shape, each rim of a cup internal diameter is 8-25mm scope, and the endoporus clear depth joining cup is 15-100mm, and the bottom of each cup aperture is semisphere.
The movement locus of suction needle described in the utility model is rectilinear motion in the horizontal direction, namely does rectilinear movement sampling along each cup of porous connection cup, diluted cup, detection cup position, distributes blood sample.
Embodiment 6
Other structure, with embodiment 2, as shown in Figure 6, adds bar magnet 23 in each cup, bottom each cup of loading stage 5, arranges stirring apparatus 21 and magnet 24, thus realize the agitating function to blood sample in each cup in porous connection cup.Loading stage 5 inside is provided with heating temperature control device 22.
In porous described in the utility model connection cup the shape of each cup and size identical, be the aggregate of the pipe line spread of 3-8 test tube shape, each rim of a cup internal diameter is 8-25mm scope, and the endoporus clear depth joining cup is 15-100mm, and the bottom of each cup aperture is semisphere.
The movement locus of suction needle described in the utility model is rectilinear motion in the horizontal direction, namely does rectilinear movement sampling along each cup of porous connection cup, diluted cup, detection cup position, distributes blood sample.
Embodiment 7
Detect the method for platelet counts in blood sample based on above-mentioned multiparameter Platelet function Analyzer, comprise the following steps:
Step 1, any induced polymerization inhibitor reagent cup in contrast is not added respectively in one of them cup of porous connection cup 4, or in this cup, load anti-coagulants in advance (as sodium ethylene diamine tetracetate, EDTA), in other each cup, add the induced polymerization inhibitor of different cultivars or the induced polymerization inhibitor of same breed variable concentrations respectively;
Step 2, by same blood sample respectively equivalent join in porous connection cup 4 each cup, slight wobble mixing makes blood sample and reagent mix and isothermal reaction at 22-37 DEG C;
Step 3, joins cup 4 and puts into porous connection cup microscope carrier 2, constantly stir and evenly mix through porous connection cup microscope carrier 2 by porous;
Step 4, UNICOM first syringe 10 and suction needle 9, draw in porous connection cup 4 blood sample to be measured contrasted in cup, add in diluted cup 6 under the drive of suction needle mobile device 1, UNICOM's pneumatic pump 13 and diluted cup 6, inject air and make blood sample and dilution fully mix and dilute;
Step 5, then the first syringe 10 blood sample of drawing in diluted cup 6 quantitative with suction needle 9 is crossed by UNICOM, adds and detects in cup 7, UNICOM's pneumatic pump 13 and detection cup 7, inject air and blood sample and dilution fully mixed and secondary dilution;
Step 6, the platelet counts of pick-up unit 8 to blood sample in contrast cup, the volume that are arranged on detection cup 7 sidewall detect and record;
Step 7, detects after terminating, instrument to diluted cup, detect cup, sampling probe and each pipeline and clean, UNICOM's waste drains pump 14 with diluted cup, detect cup, emptying waste liquid; UNICOM's dilution memory storage 12 with diluted cup, detects cup, save dilution;
Step 8, in other cup of distich cup, blood sample repeats step 4 ~ step 7, completes respectively and carries out determination and analysis to other each cup platelet counts, volume in porous connection cup.
In the utility model, pick-up unit 8 detects and record the platelet counts of blood sample in cup, calculating respectively, can obtain the aggregation rate value of same blood sample under different induced polymerization inhibitor effect respectively to detecting the platelet counts obtained according to following formula.
In the utility model, pick-up unit 8 detects and record the volume of platelets of blood sample in cup, utilize and the size of the average external volume MPV-0 value not adding induced polymerization inhibitor thromboblast is judged whether blood sample is assembled before detection, thus judge whether blood sample is assembled before detection, to avoid detecting mistake; When not adding induced polymerization inhibitor thromboblast average external volume MPV and being greater than more than 12.5fl, pick-up unit is reported to the police, and prompting may be assembled by blood sample.
Pick-up unit adopts Coulter principle (or being called theory of electrical impedance) to realize to the platelet counts in blood sample and volume detection method or implementation, and this technology is the ordinary skill in the art, and those skilled in the art are very familiar to clear.
Embodiment 8
The utility model multiparameter Platelet function Analyzer and detection method test result repeatability, and detect platelet aggregation rate results relevance with optics turbidimetry and compare.
1) (this turbidimetry is see Born GVR.Aggregation of blood platelets by adenosine diphosphate and its reversal.Nature (Lond.) .1962 for the utility model instrument and certain turbidimetry; 194:927) repeatability of Instrumental results compares.
With 1 part of sodium citrate anticoagulated whole blood for sample, detect platelet aggregation rate, each duplicate detection 10 times with instrument of the present utility model and turbidimetry instrument respectively.The results are shown in following table.
Equipment in the present embodiment is identical with the equipment in embodiment 1, and step is identical with the step of embodiment 1, wherein, the dilution used is for removing the isotonic physiological saline of particulate, induced polymerization inhibitor is adenosine diphosphate (ADP) aqueous solution, and extension rate is 100 for the first time, and second time extension rate is 100.
Table 1: the platelet aggregation rate result that the utility model instrument and turbidimetry for Determination obtain
Standard deviation (SD) and the mean value of correspondence per minute is tried to achieve respectively according to 10 measurement results.The row formulae discovery coefficient of variation (CV) is pressed again according to SD and mean value.
CV=SD/ mean value × 100%, by calculating, the CV value of this instrument is 5%, and optics turbidimetry instrument gained be my CV value is 11%, when illustrating that this instrument measures for aggregation rate, well repeated.
2) correlativity of the utility model instrument and certain turbidimetry Instrumental results
Assemble instrument Simultaneously test 40 increment originally by this instrument and traditional turbidimetry, namely two kinds of methods detect platelet aggregation rate to same sample respectively simultaneously, and 40 increments aggregation rate result is originally as follows:
Table 2: the Comparative result of the utility model instrument and turbidimetry Instrument measuring maximum platelet aggregation rate
| Sample | The utility model instrument | Certain turbidimetry assembles instrument |
| 1 | 75 | 61 |
| 2 | 56 | 50 |
| 3 | 54 | 48 |
| 4 | 66 | 59 |
| 5 | 45 | 36 |
| 6 | 69 | 61 |
| 7 | 77 | 63 |
| 8 | 48 | 40 |
| 9 | 49 | 43 |
| 10 | 78 | 62 |
| 11 | 65 | 58 |
| 12 | 51 | 40 |
| 13 | 49 | 34 |
| 14 | 62 | 49 |
| 15 | 51 | 44 |
| 16 | 65 | 53 |
| 17 | 54 | 43 |
| 18 | 46 | 42 |
| 19 | 78 | 62 |
| 20 | 68 | 54 |
| 21 | 43 | 33 |
| 22 | 65 | 59 |
| 23 | 69 | 55 |
| 24 | 39 | 30 |
| 25 | 45 | 40 |
| 26 | 57 | 45 |
| 27 | 49 | 39 |
| 28 | 46 | 39 |
| 29 | 54 | 49 |
| 30 | 34 | 25 |
| 31 | 63 | 57 |
| 32 | 68 | 59 |
| 33 | 54 | 47 |
| 34 | 53 | 39 |
| 35 | 62 | 44 |
| 36 | 51 | 37 |
| 37 | 65 | 48 |
| 38 | 59 | 53 |
| 39 | 58 | 52 |
| 40 | 46 | 42 |
With the utility model Instrumental results for x-axis, it is y-axis that turbidimetry assembles instrument measurement result, draws scatter diagram.Carry out linear regression analysis simultaneously, can Fig. 9 be obtained.From Fig. 9 result, the coefficient R of the utility model method and turbidimetry Instrumental results
2=0.8705, R=0.9330, correlativity is good.
But the result of the utility model Instrument measuring is higher than the result than turbidimetry Instrument measuring, show the result that two groups of data carry out paired-samples T-test, both have significant difference (P=0.000, P < 0.05).
The utility model quickly and easily by the mode that the blood sample automatically detecting platelet counts and process respectively contrast cup blood sample and variant induced polymerization inhibitor contrasts, can realize multiple different induced polymerization inhibitor platelet aggregation rate detection.And the information such as each detection platelet counts, mean platelet volume (MPV), erythrocyte number (RBC) and mean corpuscular volume (MCV) in detecting, can also be obtained.
The utility model provides the Platelet function Analyzer of novel multiparameter and the thinking of detection method.The method and access of this technical scheme of specific implementation is a lot; the above is only preferred implementation of the present utility model; should be understood that; for those skilled in the art; under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.The all available prior art of each ingredient not clear and definite in the present embodiment is realized.
Claims (8)
1. the novel Platelet function Analyzer of multiparameter fast, is characterized in that, described analyser comprises suction needle mobile device (1), porous connection cup microscope carrier (2), dilution and pick-up unit (3);
Suction needle mobile device (1) comprises suction needle (9) and drives the device of suction needle (9) movement, suction needle mobile device (1) is for drawing the blood sample in porous connection cup (4), transfer them in the diluted cup (6) in dilution and pick-up unit (3), and the blood sample drawn in diluted cup (6) after dilution, then transfer them to and detect in cup (7);
Porous connection cup microscope carrier (2) comprises porous connection cup (4) in loading stage (5) and loading stage, porous connection cup (4) is formed by the tube combinations of multiple test tube shape, its inside is isolated from each other, and outside is connected to an entirety;
Dilution and pick-up unit (3) comprise diluted cup (6), detect cup (7) and pick-up unit (8), diluted cup (6) and detection cup (7) are arranged side by side, and pick-up unit (8) is set at detection cup (7) sidewall, described pick-up unit (8) is for detecting quantity in blood sample to be measured in blood platelet and volume;
Wherein, porous connection cup microscope carrier (2) is arranged side by side with dilution and pick-up unit (3), and suction needle mobile device (1) is arranged on dilution and pick-up unit (3) top.
Described analyser also comprises the first syringe (10), the second syringe (11), dilution memory storage (12), pneumatic pump (13) and waste drains pump (14);
Wherein, suction needle (9) connects the first syringe (10) by pipeline, realizes drawing blood sample and distributing blood sample by the suction of the first syringe (10) and punching; Dilution memory storage (12) connects diluted cup (6) respectively by pipeline and detects cup (7), pipeline leads to diluted cup and detects cup suitable for reading, and at pipeline, valve is set close to cup-mouth end, the second syringe (11) is also provided with between dilution memory storage (12) and the pipeline of two glasss, and between dilution memory storage (12) and the second syringe (11), valve is set, for injecting dilution in two glasss;
Waste drains pump (14) connects diluted cup (6) respectively by pipeline and detects at the bottom of cup (7) cup, and arranges valve at cup bottom outlet end, and waste drains pump (14) endpiece arranges valve, for the waste liquid in emptying cup; Pneumatic pump (13) is also provided with between waste drains pump (14) and the pipeline of two glasss, and valve is set at the endpiece of pneumatic pump (13), for injecting air to diluted cup (6) and detection cup (7) bottom, blood sample and dilution is made fully to dilute respectively and mix.
2. multiparameter Platelet function Analyzer according to claim 1, it is characterized in that, suction needle mobile device (1) comprises suction needle (9), move up and down unit (15), horizontal movement unit (16), move up and down unit (15) and comprise two pulleys setting up and down, and the travelling belt between pulley, girt (17) is set between travelling belt, pull bar one end connects suction needle (9), two pulley driving travelling belts rotate, thus drive girt (17) vertical direction to move, final drive suction needle (9) vertical direction moves, horizontal movement unit (16) comprises horizontally disposed two pulleys, and the travelling belt between pulley, travelling belt with move up and down unit (15) and be connected, two pulley driving travelling belts move reciprocatingly in the horizontal direction, thus drive and move up and down unit (15) horizontal direction and move reciprocatingly, final drive suction needle (9) moves reciprocatingly in the horizontal direction, finally realizes that suction needle performs arbitrarily up and down, the to-and-fro movement of front and back.
3. multiparameter Platelet function Analyzer according to claim 1, it is characterized in that, porous connection cup microscope carrier (2) comprises loading stage (5), porous connection cup (4) of loading stage inside, two pulleys, and the travelling belt between pulley; Wherein, two pulleys are setting up and down, and loading stage sidewall arranges top pulley (18), drive loading stage (5) to rotate, below pulley (19) is driven by drive motor (20) and rotates, and is connected between two pulleys by travelling belt; During work, drive motor (20) drives below pulley (19) to rotate, thus drive top pulley (18) to rotate, rotate before and after porous connection cup (4) in final drive loading stage (5), its rotational angle is that any side is no more than 85 °, thus realizes the agitating function to blood sample in each cup.
4. multiparameter Platelet function Analyzer according to claim 1, it is characterized in that, in porous connection cup, add bar magnet (23) in each cup, stirring apparatus (21) and magnet (24) are set bottom loading stage (5) each cup, thus realize the agitating function to blood sample in each cup.
5., according to the arbitrary described multiparameter Platelet function Analyzer of Claims 1 to 4, it is characterized in that, loading stage inside is provided with heating temperature control device (22).
6. according to the arbitrary described multiparameter Platelet function Analyzer of Claims 1 to 4, it is characterized in that, the aggregate of the pipe line spread that described porous connection cup (4) is 3-8 test tube shape, in porous connection cup the shape of each cup and size identical, each cup upper internal diameter is 8-25mm, connection cup each cup endoporus clear depth is 15-100mm, and the bottom of each cup aperture is semisphere.
7. according to described multiparameter Platelet function Analyzer arbitrary in Claims 1 to 4, it is characterized in that, the movement locus of described suction needle is rectilinear motion in the horizontal direction, and the position namely distributed along each cup of porous connection cup, diluted cup, detection rim of a cup is done rectilinear movement and completed sampling, distribution, transfer blood sample.
8. according to described multiparameter Platelet function Analyzer arbitrary in Claims 1 to 4, it is characterized in that, described porous connection cup microscope carrier (2) has and join the cell texture or trench structure that cup-shaped matches with porous, join cup for safe placing porous.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122404A (en) * | 2014-08-01 | 2014-10-29 | 江苏英诺华医疗技术有限公司 | Novel rapid multi-parameter platelet functional analyzer and detection method thereof |
| CN110308271A (en) * | 2019-07-10 | 2019-10-08 | 江苏柯伦迪医疗技术有限公司 | A kind of platelet function assay system and detection method |
| CN111474001A (en) * | 2019-01-23 | 2020-07-31 | 贾莉 | A multi-functional sample verifying attachment for blood coagulation routine testing |
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2014
- 2014-08-01 CN CN201420434208.XU patent/CN204028091U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122404A (en) * | 2014-08-01 | 2014-10-29 | 江苏英诺华医疗技术有限公司 | Novel rapid multi-parameter platelet functional analyzer and detection method thereof |
| CN111474001A (en) * | 2019-01-23 | 2020-07-31 | 贾莉 | A multi-functional sample verifying attachment for blood coagulation routine testing |
| CN110308271A (en) * | 2019-07-10 | 2019-10-08 | 江苏柯伦迪医疗技术有限公司 | A kind of platelet function assay system and detection method |
| WO2021003786A1 (en) * | 2019-07-10 | 2021-01-14 | 江苏柯伦迪医疗技术有限公司 | Platelet function detection system and detection method |
| CN110308271B (en) * | 2019-07-10 | 2021-03-30 | 江苏柯伦迪医疗技术有限公司 | Platelet function detection system and detection method |
| GB2598865A (en) * | 2019-07-10 | 2022-03-16 | Sinnowa Medical Science & Tech Co Ltd | Platelet function detection system and detection method |
| GB2598865B (en) * | 2019-07-10 | 2022-07-20 | Sinnowa Medical Science & Tech Co Ltd | Platelet function detection system and detection method |
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