CN110812273B

CN110812273B - Skin barrier moisturizing and repairing cream and preparation method thereof

Info

Publication number: CN110812273B
Application number: CN201911265886.1A
Authority: CN
Inventors: 姚智荣; 王北明; 潘红炬; 廖志刚; 梁勇坤; 严高剑; 张超; 龚俊瑞; 柴将红; 余良钟; 肖娜; 陈冬梅
Original assignee: Foshan Shunde Hengxingrun Medical Technology Co ltd; Guangdong China Resources Shunfeng Pharmaceutical Co Ltd
Current assignee: Foshan Shunde Hengxingrun Medical Technology Co ltd; Guangdong China Resources Shunfeng Pharmaceutical Co Ltd
Priority date: 2019-12-11
Filing date: 2019-12-11
Publication date: 2022-11-22
Anticipated expiration: 2039-12-11
Also published as: CN110812273A

Abstract

The invention discloses a skin barrier moisturizing and repairing cream and a preparation method thereof. The skin barrier moisturizing and repairing cream comprises the following components in percentage by weight: 9 to 25 percent of oily matrix, 1 to 10 percent of lipoid compound, 1 to 5 percent of glycerin, 0.03 to 0.3 percent of sodium hyaluronate, 0.1 to 1 percent of vitamin E, 0.1 to 1 percent of allantoin, 2 to 9 percent of emulsifying system and the balance of purified water. The skin barrier moisturizing and repairing cream disclosed by the invention has the effects of moisturizing for a long time and repairing the skin barrier, has the advantages of being mild and low in irritation, and is suitable for people suffering from atopic dermatitis.

Description

Skin barrier moisturizing and repairing cream and preparation method thereof

Technical Field

The invention relates to a skin barrier moisturizing and repairing cream and a preparation method thereof, in particular to a skin barrier moisturizing and repairing cream suitable for people suffering from atopic dermatitis and a preparation method thereof.

Background

Atopic Dermatitis (AD) is a chronic, recurrent and inflammatory skin disease characterized clinically by severe itching and sleep disorders, often accompanied by Atopic diseases such as asthma, allergic rhinitis, etc., and its pathogenesis includes the breakdown of skin barrier function, external environmental factors, genetic and immunological interactions, etc.

In recent 30 years, the prevalence rate of AD has been increasing year by year, and epidemiological researches have found that the prevalence rate of AD in developed countries of Europe and America is about 20-30%, that of adults is about 10-20%, and that the prevalence rate of AD in developing countries is also increasing continuously in recent years. In 2004, AD prevalence in preschool children in China was 3.07%, while in 2014 it has reached 12.94%. Of these patients, over 75% are mild to moderate patients with dry skin, a common feature of AD patients, and eczematous lesions as the main clinical manifestations, with approximately 80% of patients presenting with xeroderma.

The current internationally accepted therapeutic strategy for atopic dermatitis is "stepwise" graded therapy, with incremental therapeutic measures being given depending on the severity of the condition of AD. However, the basic treatment of emollients is an indispensable measure, both for mild AD, which only manifests dry skin, and for refractory or severe AD. A large number of clinical studies at home and abroad prove that the treatment by using the emollient every day can obviously reduce the frequency of AD attack and shorten the time of AD attack, is an important means for preventing AD attack and is a prevention measure with high cost performance and reducing AD burden. The necessity and importance of emollient treatment is well documented in the AD treatment guidelines of europe, the united states, japan and china.

Disclosure of Invention

Based on the above, an object of the present invention is to provide a skin barrier moisturizing and repairing cream suitable for people with atopic dermatitis, which has the effects of moisturizing and repairing skin barrier for a long time, and has the advantages of mildness and low irritation.

Another object of the present invention is to provide a method for preparing the skin barrier moisturizing and repairing cream.

The technical scheme adopted by the invention is as follows:

the skin barrier moisturizing and repairing cream comprises the following components in percentage by weight:

further, the oily matrix comprises the following components in percentage by weight:

further, the emulsifying system comprises the following components in percentage by weight:

30 to 40 percent of sucrose poly stearate,

30 to 40 percent of cetearyl glucoside,

20 to 30 percent of cetearyl alcohol.

Optionally, the emulsifying system comprises the following components in percentage by weight:

further, the lipoid compound comprises the following components in percentage by weight:

wherein the oil is one or more of caprylic/capric triglyceride, dioctyl carbonate, and butanediol dicaprylate/dicaprate; the free fatty acid is selected from one or the combination of more than two of lauric acid, palmitic acid and myristic acid; the phytosterol is selected from one or the combination of more than two of beta-sitosterol, stigmasterol and campesterol.

Further, the lipoid compound also comprises the following components in percentage by weight:

further, in the lipoid compound, the thickening agent is selected from one or a combination of more than two of sodium polyacrylate, acryloyl dimethyl taurate sodium, carbomer resin, ammonium taurine/behenyl alcohol polyether-25, methacrylate cross-linked polymer, sodium polyacrylate grafted starch and polyvinylpyrrolidone; the preservative is selected from one or the combination of more than two of hexanediol, p-hydroxyacetophenone, phenoxyethanol, hydroxyphenyl esters, pentanediol, caprylyl hydroximic acid and ethylhexyl glycerol; the antioxidant is selected from one or a combination of more than two of dibutyl hydroxy toluene (BHT), butyl Hydroxy Anisole (BHA) and sodium sulfite; the chelating agent is one or more of ethylenediaminetetraacetic acid (EDTA), disodium ethylenediaminetetraacetate (EDTA-2 Na), tetrasodium ethylenediaminetetraacetate (EDTA-4 Na), citric acid and sodium citrate.

Optionally, the lipid complex comprises the following components in percentage by weight:

further, the skin barrier moisturizing and repairing cream further comprises the following components in percentage by weight:

further, in the skin barrier moisturizing and repairing cream, the thickening agent is selected from one or a combination of more than two of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer, sodium polyacrylate, acryloyl dimethyl taurate, carbomer resin, methacrylate cross-linked polymer, sodium polyacrylate grafted starch and polyvinylpyrrolidone; the preservative is selected from one or more of hexanediol, p-hydroxyacetophenone, phenoxyethanol, hydroxyphenyl esters, pentanediol, caprylyl hydroximic acid and ethylhexyl glycerol; the antioxidant is selected from one or a combination of more than two of dibutyl hydroxy toluene (BHT), butyl Hydroxy Anisole (BHA) and sodium sulfite; the chelating agent is one or more of ethylenediaminetetraacetic acid (EDTA), disodium ethylenediaminetetraacetate (EDTA-2 Na), tetrasodium ethylenediaminetetraacetate (EDTA-4 Na), citric acid and sodium citrate.

Optionally, the skin barrier moisturizing and repairing cream comprises the following components in percentage by weight:

the preparation method of the skin barrier moisturizing and repairing cream comprises the following steps:

adding a thickening agent and sodium hyaluronate into glycerol, and uniformly stirring to obtain a mixed solution;

taking an oil-based matrix, adding an antioxidant and an emulsification system, stirring, heating and dissolving to obtain an oil phase;

adding allantoin, a preservative and a chelating agent into purified water, stirring and heating uniformly, adding the mixed solution, stirring uniformly, adding the oil phase, emulsifying and homogenizing, adding vitamin E and a lipoid compound, and stirring uniformly to obtain the skin barrier moisturizing and repairing cream.

Further, the preparation method of the oily matrix comprises the following steps: mixing caprylic/capric triglyceride, squalane, shea butter, polydimethylsiloxane and olive oil unsaponifiable matter under heating condition, and stirring to obtain the oily matrix.

Further, the preparation method of the lipoid compound comprises the following steps:

respectively taking grease, ceramide 3, free fatty acid, phytosterol, an antioxidant and a thickening agent, and stirring and mixing uniformly under a heating condition to obtain an oil phase;

respectively adding hydrogenated phosphatidylcholine, glycerol, a chelating agent and a preservative into purified water, and stirring and mixing uniformly under a heating condition to obtain a water phase;

slowly adding the oil phase into the water phase under heating, and stirring along the same direction to obtain an emulsified matrix;

and cooling after emulsification is finished, then adding the vitamin E into the emulsified matrix, and uniformly stirring and mixing to obtain the lipoid compound.

The skin barrier moisturizing and repairing cream disclosed by the invention has the effects of quickly reducing the moisture of the skin, increasing the content of the horny layer and repairing the skin barrier, has the advantages of being mild, low in irritation and the like, and is suitable for daily skin moisturizing and repairing treatment of atopic dermatitis crowds.

The formula is designed by selecting physiological lipid such as ceramide 3, free fatty acid, phytosterol and the like according to the proportion of the lipid components of the stratum corneum of the skin, and a bionic lipid compound is constructed to provide a material basis for supplementing the physiological lipid of the stratum corneum and repairing the skin barrier; meanwhile, glycerin, hyaluronic acid, vitamin E and the like are selected to form a moisturizing system, and then a grease moisturizing system is matched, so that the synergistic effect of the three parts can effectively reduce the percutaneous moisture loss of the skin, repair the skin cutin barrier and greatly improve the moisturizing effect; in addition, in order to stabilize the relationship among the three and accord with the mild design concept, the formula adopts a unique emulsifying system, a compact liquid crystal structure can be formed, the use amount of the humectant can be greatly reduced while the same moisturizing effect is achieved, and the irritation of the product is greatly reduced. The complex components such as essence, plant extracts and the like are not added in the formula, so that the irritation and sensitization of the formula can be further reduced.

The lipoid compound in the formula selects the bionic ceramide 3, the free fatty acid and the phytosterol to simulate the proportion of lipid among cells of the stratum corneum of the skin to carry out proportioning, and the ceramide, the free fatty acid and the phytosterol are mutually matched to enable the ceramide, the free fatty acid and the phytosterol molecules to be jointly inlaid among the phosphatidylcholine molecules to form a cross flower liquid crystal structure which is orderly arranged, so that a bionic skin barrier structure is constructed, the skin barrier structure is more attached to the skin of a human body, the water content of the stratum corneum of the skin can be prolonged, and a good skin repairing effect is obtained. In addition, the caprylic/capric triglyceride has good spreadability, compatibility and safety, can make the oily components in the formula well dissolved, can improve the extensibility of the formula, can also be used as a fat-endowing agent, supplements the necessary lipid of the skin, and has the effects of lubricating and softening the skin.

The emulsifying system in the formula selects sucrose polystearate and cetearyl glucoside, or sucrose polystearate and sodium stearoyl lactylate as main emulsifiers, selects cetearyl alcohol and/or glyceryl stearate as auxiliary emulsifiers, and can form a compact liquid crystal structure through reasonable compounding of the two emulsifiers. The sucrose polystearate has a structure with a plurality of hydrophobic long chain group tails and a relatively small hydrophilic group head, and the structures of cetearyl glucoside and sodium stearoyl lactylate have a hydrophobic long chain group tail and a relatively large hydrophilic group head, and the hydrophobic group and hydrophilic group structures of the two can form proper critical stacking parameters under proper proportion, thereby forming a liquid crystal structure. However, the structural compactness of the two is affected due to the steric hindrance effect between the hydrophilic groups of the two. The structure of the cetearyl alcohol and the glyceryl stearate has a high alcohol fat long chain similar to that of the main emulsifier or a similar lipophilic group and a very small hydrophilic group, so that the steric effect is smaller

The emulsifier is added into a liquid crystal system of the main emulsifier, so that a more compact and ordered liquid crystal structure is formed. Meanwhile, the hydrophilic group in the system can form gel network combination with water, and the network structure of the gel network enables the mobile phase to be fixed to a certain degree, effectively prevents flocculation, thereby improving the productStability of (2). The more compact liquid crystal structure has larger storage capacity to the water phase, on one hand, more water phases can be locked, and on the other hand, the slow release effect is realized on the release of water, so that the effects of improving the water content (MSC) of the stratum corneum and reducing the percutaneous water loss (TWEL) are realized.

The oily matrix in the formula adopts caprylic/capric triglyceride, squalane and other components, is grease with good spreadability, and simultaneously controls the dosage of the oily matrix to be 9-25%, so that on one hand, the oily matrix can bring better skin feel, on the other hand, the oily matrix can be matched with a liquid crystal emulsification system to form better physical stability and skin affinity, has moderate sealing effect, is refreshing and not greasy, and can synergistically reduce the loss of skin moisture through skin. In addition, the formula adopts acryloyl dimethyl ammonium taurate/vinyl pyrrolidone copolymer (acryloyl dimethyl ammonium taurate/VP copolymer), so that the emulsion stabilizing effect is achieved, the stability of an emulsifying system can be improved, and the irritation is very low. The moisturizing system is composed of glycerol, sodium hyaluronate and vitamin E, has better water locking and moisturizing and slow-release capabilities under the action of an emulsifying system, and can effectively reduce the percutaneous water loss of skin and improve the water content of stratum corneum; meanwhile, the dosage of the glycerol can be reduced to be within 5 percent, the irritation caused by overhigh dosage of the glycerol is avoided, the dosage of the sodium hyaluronate can be reduced to be within 0.3 percent, and the phenomenon that the rheological property of the product is damaged due to overhigh dosage of the sodium hyaluronate is avoided. Moreover, compared with the traditional preservative system, the preservative system formed by reasonably proportioning the hexanediol and the p-hydroxyacetophenone can also greatly reduce the irritation of the product.

The skin barrier moisturizing and repairing cream disclosed by the invention has the advantages that a compact liquid crystal structure is formed together through the interaction between the grease moisturizing system, the emulsifying system and the lipoid compound, and a good moisturizing effect can be generated; meanwhile, due to the structural design of the bionic lamellar liquid crystal of the lipoid compound, the lipid compound can effectively supplement physiological lipid for the horny layer, repair the lipid barrier function of the horny layer of the skin, further reduce the loss of percutaneous water and improve the water content of the horny layer, thereby having good skin repair effect. During the preparation process, the emulsion formed by the formula can obtain a stable final product through one phase transformation, can show good thixotropy (shear thinning), and can release the oil-phase component and the water-phase component simultaneously when being coated and used.

Drawings

FIG. 1 is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example six, FIG. 1A is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example three, FIG. 1B is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example four, and FIG. 1C is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example five;

FIG. 2 is a graph showing the results of the transdermal water loss test in example six.

Fig. 3 is a graph showing the results of the test for increasing the water content of the horny layer according to the sixth embodiment.

Detailed Description

The first embodiment is as follows: preparation of lipid complexes

Weighing the components according to the following formula:

the preparation steps are as follows:

adding caprylic/capric triglyceride into ceramide 3, lauric acid, beta-sitosterol and sodium polyacrylate respectively, stirring and heating to 90-100 ℃ for dissolving, keeping the temperature and stirring for 10-20 minutes to uniformly mix, and cooling to 75-85 ℃ to obtain an oil phase;

taking purified water, respectively adding hydrogenated phosphatidylcholine, glycerol, disodium ethylene diamine tetraacetate and p-hydroxyacetophenone, stirring and heating to 90-100 ℃ for dissolving, keeping the temperature and stirring for 10-20 minutes to uniformly mix, and cooling to 75-85 ℃ to obtain a water phase;

adding the oil phase into the water phase in a trickle shape at 75-85 ℃, and carrying out heat preservation, stirring and homogenizing for 10-20 minutes along the same direction to obtain an emulsified matrix;

after emulsification is finished, stirring and cooling to below 45 ℃, then adding hexanediol and vitamin E into an emulsifying matrix, stirring uniformly, and continuously cooling to below 38 ℃ to obtain the lipoid liquid crystal compound.

The prepared lipoid liquid crystal compound is observed under a polarizing microscope, so that molecules such as ceramide 3, lauric acid, beta-sitosterol and the like are embedded in a lamellar liquid crystal body of the hydrogenated phosphatidylcholine together to form a cross-shaped liquid crystal polarizing structure which is orderly arranged.

The liquid crystal is formed because the liquid crystal emulsifier and the auxiliary emulsifier form an interface film with ordered layers, compactness and viscoelasticity on an oil/water interface, the auxiliary emulsifier and the liquid crystal emulsifier which are properly proportioned are added, the ordered arrangement of the layers can be more favorably formed, and the hydrophilic group and water molecules form a gel network to be combined, so that the formula is more stable.

The second embodiment: preparation of an oily base

The components are respectively weighed according to the following formula:

the preparation steps are as follows:

and (2) adding caprylic/capric triglyceride into squalane, shea butter, polydimethylsiloxane and olive oil unsaponifiable matter respectively, stirring and heating to 90-100 ℃, and uniformly mixing to obtain the oily matrix.

Example three: preparation of skin barrier moisturizing and repairing cream

The components are respectively weighed according to the following formula:

the preparation steps are as follows:

adding acryloyl dimethyl ammonium taurate/VP copolymer, sodium polyacrylate and sodium hyaluronate into glycerol, and uniformly stirring to obtain a mixed solution;

taking an oily matrix, adding dibutyl hydroxy toluene, sucrose polystearate, cetearyl glucoside and cetearyl alcohol, stirring and heating to 90-100 ℃ for dissolving, keeping the temperature and stirring for 10-20 minutes, and then cooling to 75-85 ℃ to obtain an oil phase;

adding allantoin, p-hydroxyacetophenone and disodium ethylene diamine tetraacetate into purified water, stirring and heating to 90-100 ℃ for dissolving, preserving heat and stirring for 10-20 minutes, cooling to 75-85 ℃, adding the mixed solution, stirring for 10-20 minutes, homogenizing for 10-20 minutes, then adding the oil phase, stirring and emulsifying for 10-20 minutes, homogenizing for 10-20 minutes, cooling to below 45 ℃, adding hexanediol, vitamin E and lipoid compound, stirring for 10-20 minutes, homogenizing for 10-20 minutes, and cooling to below 40 ℃, thus obtaining the skin barrier moisturizing and repairing cream.

Example four: preparation of skin barrier moisturizing and repairing cream

The components are respectively weighed according to the following formula:

the preparation steps are as follows:

adding acryloyl dimethyl ammonium taurate/VP copolymer and sodium hyaluronate into glycerol, and uniformly stirring to obtain a mixed solution;

taking an oily matrix, adding dibutyl hydroxy toluene, sucrose polystearate, sodium stearoyl lactylate, cetostearyl alcohol and glyceryl stearate, stirring and heating to 90-100 ℃ for dissolving, keeping the temperature and stirring for 10-20 minutes, and then cooling to 75-85 ℃ to obtain an oil phase;

Example five: preparation of traditional skin barrier moisturizing and repairing cream

Weighing the components according to the following formula:

the preparation steps are as follows:

adding sodium hyaluronate into glycerol, and stirring to obtain mixed solution;

taking neopentyl glycol diheptanoate, squalane, behenyl alcohol, C12-20 alkyl glucoside, cetearyl alcohol olive oleate, ceramide 3, cholesterol, octyldodecanol and dibutyl hydroxy toluene, stirring and heating to 90-100 ℃ for dissolving, continuing stirring for 10-20 minutes at the temperature, and then cooling to 75-85 ℃ to obtain an oil phase;

adding hydrogenated lecithin, glyceryl polyether-26, betaine, glyceryl polyacrylate, allantoin, p-hydroxyacetophenone and disodium ethylene diamine tetraacetate into purified water, stirring and heating to 90-100 ℃ for dissolution, continuously keeping the temperature and stirring for 10-20 minutes, cooling to 75-85 ℃, respectively adding the mixed solution and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, stirring for 10-20 minutes, homogenizing for 10-20 minutes, adding an oil phase, stirring and emulsifying for 10-20 minutes, homogenizing for 10-20 minutes, cooling to below 45 ℃, adding vitamin E and hexanediol, continuously stirring and emulsifying for 10-20 minutes, homogenizing for 10-20 minutes, and cooling to below 40 ℃ to obtain the traditional skin barrier moisturizing repair cream.

Example six: performance test

1. Irritation test

1) Chick embryo chorioallantoic membrane blood vessel test (CAM test)

The skin barrier moisturizing and repairing creams prepared in the third, fourth and fifth examples are respectively taken for chicken embryo chorioallantoic membrane blood vessel tests, and the blood vessel changes of the allantoic membrane after being exposed to the skin barrier moisturizing and repairing cream are observed.

The test method comprises the following steps: 40ul of test samples are taken and directly dripped into a test ring on the surface of chorioallantoic membrane in the middle stage of fertilized chick embryos of 10-14 days of embryo age, and after the test ring is cultured in an incubator at 37 ℃ for 30 minutes, the effects of CAM blood vessels and the degree of each effect are recorded.

And (3) test results: as shown in fig. 1A, 1B and 1C, the skin barrier moisturizing and conditioning creams of examples three and four showed no irritation in the CAM test, were extremely mild in nature, and did not cause bleeding damage to the chorioallantoic membrane vessels of chicken embryo hair; the skin barrier moisturizer of example five showed mild irritation in the CAM test, causing mild bleeding damage to the chorioallantoic vessels of chicken embryo hair.

And (4) judging the standard:

2) Multiple skin irritation test

According to the method of "skin irritation/corrosion test" in the technical code for cosmetic safety "2015 edition, the skin barrier moisturizing and repairing creams prepared in the third and fourth examples were taken, and rabbits were used as test animals to perform a skin irritation test.

The test method comprises the following steps: the test samples were applied to the skin of test animals (rabbits), wherein the sample groups were coated with the skin barrier moisturizing creams of example three and example four, respectively, and the blank group was coated with purified water, and the skin irritation degree of the test animals was observed and scored at regular time intervals.

And (4) judging the standard:

and (3) test results:

the test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment are non-irritant and have extremely mild properties.

3) Patch test on human skin

According to the method of the ' test for skin spots on human body ' in the technical Specification for safety of cosmetics ' 2015 edition, the skin barrier moisturizing and repairing creams prepared in the third and fourth examples are respectively taken for the test for skin spots on human body.

The test method comprises the following steps: selecting 30 subjects with an area of 50mm or less ² And a spot tester with the depth not more than 1mm, wherein 0.02 g-0.025 g of test sample is smeared in the spot tester and then is pasted on the back of the subject. Wherein, the sample groups were coated with the skin barrier moisturizing cream of example three and example four, respectively, and the blank group was coated with purified water. After 24 hours, the plaque tester was removed, and the skin reaction of the subject was observed at 0.5 hour, 24 hours, and 48 hours after the removal, respectively, and the results of the skin reaction grade were recorded.

And (4) judging the standard: skin closed type spot test skin reaction grade standard

And (3) test results:

the test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment have no allergic reaction in the human skin patch test.

2. Percutaneous Water loss (TWEL) test

The skin barrier moisturizing and repairing creams prepared in the third, fourth and fifth examples were respectively taken and human bodies were used as parameter objects (20 persons in each group of subjects) to carry out the transdermal water loss test.

The test method comprises the following steps: after the test site (inner side of front arm) was washed by the subject, the test site was marked with a test area (test area about 4 cm) ² ) And one arm is used as blank control, and the corresponding area of the other arm is smeared with the test sample. Under the condition of a constant temperature and humidity environment, after a subject enters a testing room and sits statically for 30 minutes, a percutaneous water loss measuring instrument is adopted to carry out initial reading on a testing area and a blank control area respectively; then, weighing a proper amount of test sample, uniformly coating the test sample in the test area, not processing the blank control area, reading the test area at regular intervals, and recording data.

And (3) test results:

test results show that after the skin barrier moisturizing and repairing cream provided by the third embodiment and the fourth embodiment of the invention is used, the percutaneous water loss can be quickly reduced and can be maintained for a long time, and the skin barrier moisturizing and repairing cream is obviously superior to the traditional skin barrier moisturizing and repairing cream provided by the fifth embodiment.

3. Stratum corneum water content (MSC) lifting test

The skin barrier moisturizing and repairing creams prepared in the third, fourth and fifth examples were respectively used for stratum corneum water content increase test with human body as a parameter object (20 subjects per group).

The test method comprises the following steps: after the test site (for example, arm, forehead, etc.) is cleaned by the subject, the test site is circled to make a test area mark (test area is about 4 cm) ² ) And selecting the area with the same size above the blank area as the blank control area. Under the condition of a constant temperature and humidity environment, after a subject enters a testing room and sits for 30 minutes, a stratum corneum water content tester is adopted to carry out initial reading on a testing area and a blank control area respectively; then, weighing a proper amount of test sample, uniformly coating the test sample in the test area, not processing the blank control area, reading the test area at regular intervals, and recording data.

And (3) test results:

test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment improve the moisture content of the stratum corneum by over 80 percent; the skin barrier moisturizing treatment cream of example five increased the moisture content of the stratum corneum by only about 60%.

4. Stability test

1) Accelerated test at 40 deg.C

The test method comprises the following steps: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples are respectively placed in a constant temperature box at 40 ℃, and the pH value, the uniformity, the color change and the centrifugation are examined within 3 months and compared with the samples at the normal temperature for 0 day.

And (3) test results: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples have good uniformity, no delamination after centrifugation and no obvious difference in pH value and color change compared with the samples obtained after 0 day at normal temperature under the condition of 3 months at 40 ℃.

2) Low temperature test at-15 deg.C

The test method comprises the following steps: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples are placed in an incubator at-15 ℃ respectively, and the pH value, uniformity, discoloration and centrifugation conditions of the skin barrier moisturizing and repairing creams are examined within 1 month and compared with the samples at the normal temperature for 0 days.

And (3) test results: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples have good uniformity, no delamination after centrifugation and no obvious difference in pH value and color change compared with the samples obtained after 0 day at normal temperature under the condition of 1 month at-15 ℃.

3) Cold and hot cycle test

The test method comprises the following steps: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples are placed in an incubator at 60 ℃ for 24 hours and then in an incubator at-15 ℃ for 24 hours, and repeated for 9 times, and the properties, pH value and consistency of the product recovered to room temperature are examined and compared with the samples at room temperature for 0 day.

And (3) test results: after 9 times of cold and hot cycles, the skin barrier moisturizing and repairing creams prepared in the third and fourth examples are uniform, fine and smooth in cream body, and have no obvious difference in pH value and consistency compared with the samples obtained after 0 day at normal temperature.

The stability test result shows that the skin barrier moisturizing and repairing cream prepared in the third example and the fourth example has good stability.

5. Test for Corrosion resistance

The skin barrier moisturizing treatment creams prepared in example three and example four were respectively tested for antisepsis according to the microbial antisepsis efficacy test method of United states pharmacopoeia USP32<51 >. The test strains included: bacteria (Pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, burkholderia cepacia) and molds (Candida albicans, aspergillus niger).

And (4) testing standard:

and (3) testing results:

the test results show that the bacterial mold of the preservative system of the skin barrier moisturizing and repairing cream of the third example and the fourth example does not increase any more after 14 days, and the reduction log value is all more than 2.

6. Risk substance testing

The skin barrier moisturizing repair creams prepared in example three and example four were subjected to the risk substance content test according to the risk substance test method of the cosmetic safety technical code 2015 edition.

And (3) testing results:

the test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment have the content of risk substances far smaller than the limit of national standards.

The comprehensive test results show that the skin barrier moisturizing and repairing cream prepared by the invention can effectively reduce the percutaneous water loss and improve the water content of the stratum corneum, has the effect of repairing the skin barrier, and has the advantages of mild property and low irritation.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. The skin barrier moisturizing and repairing cream is characterized by comprising the following components in percentage by weight:

and the balance purified water;

or the composition comprises the following components in percentage by weight:

and the balance purified water;

wherein the oily matrix comprises the following components in percentage by weight:

the lipoid compound comprises the following components in percentage by weight:

and the balance purified water.

2. The skin barrier moisturizing and repairing cream as claimed in claim 1, wherein the skin barrier moisturizing and repairing cream further comprises the following components by weight percent:

wherein the thickening agent is selected from one or the combination of more than two of acryloyl dimethyl ammonium taurate/vinyl pyrrolidone copolymer, sodium polyacrylate, acryloyl dimethyl sodium taurate, carbomer resin, methacrylate cross-linked polymer, sodium polyacrylate grafted starch and polyvinylpyrrolidone; the preservative is selected from one or the combination of more than two of hexanediol, p-hydroxyacetophenone, phenoxyethanol, hydroxyphenyl esters, pentanediol, caprylyl hydroximic acid and ethylhexyl glycerol; the antioxidant is selected from one or a combination of more than two of dibutyl hydroxy toluene, butyl hydroxy anisol and sodium sulfite; the chelating agent is one or more of ethylenediamine tetraacetic acid, disodium ethylenediamine tetraacetic acid, tetrasodium ethylenediamine tetraacetic acid, citric acid and sodium citrate.

3. The skin barrier moisturizing and repairing cream as claimed in claim 2, wherein the skin barrier moisturizing and repairing cream comprises the following components by weight percent:

and the balance purified water;

wherein, the lipoid compound comprises the following components in percentage by weight:

and the balance purified water.

4. The skin barrier moisturizing and repairing cream as claimed in claim 2, wherein the skin barrier moisturizing and repairing cream comprises the following components by weight percent: