CN110812273A

CN110812273A - Skin barrier moisturizing and repairing cream and preparation method thereof

Info

Publication number: CN110812273A
Application number: CN201911265886.1A
Authority: CN
Inventors: 姚智荣; 王北明; 潘红炬; 廖志刚; 梁勇坤; 严高剑; 张超; 龚俊瑞; 柴将红; 余良钟; 肖娜; 陈冬梅
Original assignee: Foshan Shunde Hengxingrun Medical Technology Co Ltd; Guangdong China Resources Shunfeng Pharmaceutical Co Ltd
Current assignee: Foshan Shunde Hengxingrun Medical Technology Co Ltd; Guangdong China Resources Shunfeng Pharmaceutical Co Ltd
Priority date: 2019-12-11
Filing date: 2019-12-11
Publication date: 2020-02-21
Anticipated expiration: 2039-12-11
Also published as: CN110812273B

Abstract

The invention discloses a skin barrier moisturizing and repairing cream and a preparation method thereof. The skin barrier moisturizing and repairing cream comprises the following components in percentage by weight: 9 to 25 percent of oily matrix, 1 to 10 percent of lipoid compound, 1 to 5 percent of glycerin, 0.03 to 0.3 percent of sodium hyaluronate, 0.1 to 1 percent of vitamin E, 0.1 to 1 percent of allantoin, 2 to 9 percent of emulsifying system and the balance of purified water. The skin barrier moisturizing and repairing cream disclosed by the invention has the effects of moisturizing for a long time and repairing the skin barrier, has the advantages of being mild and low in irritation, and is suitable for people suffering from atopic dermatitis.

Description

Skin barrier moisturizing and repairing cream and preparation method thereof

Technical Field

The invention relates to a skin barrier moisturizing and repairing cream and a preparation method thereof, in particular to a skin barrier moisturizing and repairing cream suitable for people suffering from atopic dermatitis and a preparation method thereof.

Background

Atopic Dermatitis (AD) is a chronic, recurrent and inflammatory skin disease characterized clinically by severe itching and sleep disorders, often accompanied by Atopic diseases such as asthma, allergic rhinitis, etc., and its pathogenesis includes the breakdown of skin barrier function, external environmental factors, genetic and immunological interactions, etc.

In recent 30 years, the prevalence rate of AD is rising year by year, epidemiological research shows that the prevalence rate of AD in developed countries of Europe and America is about 20-30%, and that the prevalence rate of AD in developed countries of Europe and America is about 10-20%, and the prevalence rate of AD in developing countries is also continuously rising in recent years. In 2004, AD prevalence in preschool children in China was 3.07%, while in 2014 it has reached 12.94%. Of these patients, over 75% are mild to moderate patients with dry skin, a common feature of AD patients, and eczematous lesions as the main clinical manifestations, with approximately 80% of patients presenting dry skin.

The current internationally accepted therapeutic strategy for atopic dermatitis is "stepwise" graded therapy, with incremental therapeutic measures being given depending on the severity of the condition of AD. However, the basic treatment of emollients is an indispensable measure, both for mild AD, which only manifests dry skin, and for refractory or severe AD. A large number of clinical studies at home and abroad prove that the treatment by using the emollient every day can obviously reduce the frequency of AD attack and shorten the time of AD attack, is an important means for preventing AD attack and is a prevention measure with high cost performance and reducing AD burden. The necessity and importance of emollient treatment is well documented in the AD treatment guidelines of europe, the united states, japan and china.

Disclosure of Invention

Based on the above, an object of the present invention is to provide a skin barrier moisturizing and repairing cream suitable for people with atopic dermatitis, which has the effects of moisturizing and repairing skin barrier for a long time, and has the advantages of mildness and low irritation.

Another object of the present invention is to provide a method for preparing the skin barrier moisturizing and repairing cream.

The technical scheme adopted by the invention is as follows:

the skin barrier moisturizing and repairing cream comprises the following components in percentage by weight:

and the balance purified water.

Further, the oily matrix comprises the following components in percentage by weight:

further, the emulsifying system comprises the following components in percentage by weight:

30 to 40 percent of sucrose polystearate,

30 to 40 percent of cetearyl glucoside,

20 to 30 percent of cetearyl alcohol.

Optionally, the emulsifying system comprises the following components in percentage by weight:

further, the lipoid compound comprises the following components in percentage by weight:

and the balance purified water;

the oil is selected from one or a combination of more than two of caprylic/capric triglyceride, dicaprylyl carbonate and butanediol dicaprylate/dicaprate, the free fatty acid is selected from one or a combination of more than two of lauric acid, palmitic acid and myristic acid, and the phytosterol is selected from one or a combination of more than two of β -sitosterol, stigmasterol and campesterol.

Further, the lipoid compound also comprises the following components in percentage by weight:

further, in the lipoid compound, the thickening agent is selected from one or a combination of more than two of sodium polyacrylate, acryloyl dimethyl taurate sodium, carbomer resin, ammonium taurine/behenyl alcohol polyether-25, methacrylate cross-linked polymer, sodium polyacrylate grafted starch and polyvinylpyrrolidone; the preservative is selected from one or more of hexanediol, p-hydroxyacetophenone, phenoxyethanol, hydroxyphenyl esters, pentanediol, caprylyl hydroximic acid and ethylhexyl glycerol; the antioxidant is selected from one or more of dibutyl hydroxy toluene (BHT), Butyl Hydroxy Anisol (BHA) and sodium sulfite; the chelating agent is one or more of ethylenediaminetetraacetic acid (EDTA), disodium ethylenediaminetetraacetate (EDTA-2Na), tetrasodium ethylenediaminetetraacetate (EDTA-4Na), citric acid and sodium citrate.

Optionally, the lipid complex comprises the following components in percentage by weight:

and the balance purified water.

Further, the skin barrier moisturizing and repairing cream further comprises the following components in percentage by weight:

further, in the skin barrier moisturizing and repairing cream, the thickening agent is selected from one or a combination of more than two of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer, sodium polyacrylate, acryloyl dimethyl taurate, carbomer resin, methacrylate cross-linked polymer, sodium polyacrylate grafted starch and polyvinylpyrrolidone; the preservative is selected from one or more of hexanediol, p-hydroxyacetophenone, phenoxyethanol, hydroxyphenyl esters, pentanediol, caprylyl hydroximic acid and ethylhexyl glycerol; the antioxidant is selected from one or more of dibutyl hydroxy toluene (BHT), Butyl Hydroxy Anisol (BHA) and sodium sulfite; the chelating agent is one or more of ethylenediaminetetraacetic acid (EDTA), disodium ethylenediaminetetraacetate (EDTA-2Na), tetrasodium ethylenediaminetetraacetate (EDTA-4Na), citric acid and sodium citrate.

Optionally, the skin barrier moisturizing and repairing cream comprises the following components in percentage by weight:

and the balance purified water.

The preparation method of the skin barrier moisturizing and repairing cream comprises the following steps:

adding a thickening agent and sodium hyaluronate into glycerol, and uniformly stirring to obtain a mixed solution;

taking an oil-based matrix, adding an antioxidant and an emulsification system, stirring, heating and dissolving to obtain an oil phase;

adding allantoin, a preservative and a chelating agent into purified water, stirring and heating uniformly, adding the mixed solution, stirring uniformly, adding the oil phase, emulsifying and homogenizing, adding vitamin E and a lipoid compound, and stirring uniformly to obtain the skin barrier moisturizing and repairing cream.

Further, the preparation method of the oily matrix comprises the following steps: mixing caprylic/capric triglyceride, squalane, shea butter, polydimethylsiloxane and olive oil unsaponifiable matter under heating condition, and stirring to obtain the oily matrix.

Further, the preparation method of the lipoid compound comprises the following steps:

respectively taking oil, ceramide 3, free fatty acid, phytosterol, an antioxidant and a thickening agent, and stirring and mixing uniformly under a heating condition to obtain an oil phase;

adding hydrogenated phosphatidylcholine, glycerol, chelating agent and preservative into purified water respectively, and stirring and mixing uniformly under heating conditions to obtain a water phase;

under the heating condition, slowly adding the oil phase into the water phase, and stirring along the same direction to obtain an emulsified matrix;

and cooling after emulsification is finished, then adding the vitamin E into the emulsified matrix, and uniformly stirring and mixing to obtain the lipoid compound.

The skin barrier moisturizing and repairing cream disclosed by the invention has the effects of quickly reducing the moisture of the skin, increasing the content of the horny layer and repairing the skin barrier, has the advantages of being mild, low in irritation and the like, and is suitable for daily skin moisturizing and repairing treatment of atopic dermatitis crowds.

The formula is designed by selecting physiological lipid such as ceramide 3, free fatty acid, phytosterol and the like according to the proportion of the lipid components of the stratum corneum of the skin, and a bionic lipid compound is constructed to provide a material basis for supplementing the physiological lipid of the stratum corneum and repairing the skin barrier; meanwhile, glycerin, hyaluronic acid, vitamin E and the like are selected to form a moisturizing system, and then a grease moisturizing system is matched, so that the three parts have mutual synergistic effect, the percutaneous moisture loss of the skin can be effectively reduced, the skin cutin barrier can be repaired, and the moisturizing effect is greatly improved; in addition, in order to stabilize the relationship among the three and accord with the mild design concept, the formula adopts a unique emulsifying system, a compact liquid crystal structure can be formed, the same moisturizing effect can be achieved, the using amount of the moisturizing agent can be greatly reduced, and the irritation of the product can be greatly reduced. The complex components such as essence, plant extracts and the like are not added in the formula, so that the irritation and sensitization of the formula can be further reduced.

The lipoid compound in the formula selects the bionic ceramide 3, the free fatty acid and the phytosterol to simulate the proportion of lipid among cells of the stratum corneum of the skin to carry out proportioning, and the ceramide, the free fatty acid and the phytosterol are mutually matched to enable the ceramide, the free fatty acid and the phytosterol molecules to be jointly inlaid among the phosphatidylcholine molecules to form a cross flower liquid crystal structure which is orderly arranged, so that a bionic skin barrier structure is constructed, the skin barrier structure is more attached to the skin of a human body, the water content of the stratum corneum of the skin can be prolonged, and a good skin repairing effect is obtained. In addition, the caprylic/capric triglyceride has good spreadability, compatibility and safety, can make the oily components in the formula well dissolved, can improve the extensibility of the formula, can also be used as a fat-endowing agent, supplements the necessary lipid of the skin, and has the effects of lubricating and softening the skin.

The emulsifying system in the formula selects sucrose polystearate and cetearyl glucoside, or sucrose polystearate and sodium stearoyl lactylate as main emulsifiers, selects cetearyl alcohol and/or glyceryl stearate as auxiliary emulsifiers, and can form a compact liquid crystal structure through reasonable compounding of the two emulsifiers. Wherein, the sucrose polystearate has a structure with a plurality of long chain group tails with hydrophobicity and a relatively small head of hydrophilic group, the cetearyl glucoside and the sodium stearoyl lactylate have a structure with a long chain group tail with hydrophobicity and a relatively large head of hydrophilic group, and the hydrophobic group and the hydrophilic group of the two areThe structure can form proper critical stacking parameters under proper proportion, thereby forming a liquid crystal structure. However, the structural compactness of the two is affected due to the steric hindrance effect between the hydrophilic groups of the two. The structure of the cetearyl alcohol and the glyceryl stearate has a high alcohol fat long chain similar to that of the main emulsifier or a similar lipophilic group and a very small hydrophilic group, so that the steric effect is smaller

The emulsifier is added into a liquid crystal system of the main emulsifier, so that a more compact and ordered liquid crystal structure is formed. Meanwhile, the hydrophilic group in the system can form a gel network with water to combine, and the network structure of the gel network enables the mobile phase to be fixed to a certain extent, so that the flocculation effect is effectively prevented, and the stability of the product is improved. The storage capacity of a more compact liquid crystal structure to the water phase is also larger, on one hand, more water phases can be locked, and on the other hand, the slow release effect is realized on the release of water, so that the effects of improving the water content of the stratum corneum (MSC) and reducing the percutaneous water loss (TWEL) are realized.

The oily matrix in the formula adopts caprylic/capric triglyceride, squalane and other components, is grease with good spreadability, and simultaneously controls the dosage of the oily matrix to be 9-25%, so that on one hand, the oily matrix can bring better skin feel, on the other hand, the oily matrix can be matched with a liquid crystal emulsification system to form better physical stability and skin affinity, has moderate sealing effect, is refreshing and not greasy, and can synergistically reduce the loss of skin moisture through skin. In addition, an acryloyl dimethyl ammonium taurate/vinyl pyrrolidone copolymer (acryloyl dimethyl ammonium taurate/VP copolymer) is adopted in the formula, so that the preparation method has the function of stabilizing the suspended emulsion, can improve the stability of an emulsification system, and has extremely low irritation. The moisturizing system is composed of glycerol, sodium hyaluronate and vitamin E, has better water locking and moisturizing and slow-release capabilities under the action of an emulsifying system, and can effectively reduce the percutaneous water loss of skin and improve the water content of stratum corneum; meanwhile, the dosage of the glycerol can be reduced to be within 5 percent, the irritation caused by overhigh dosage of the glycerol is avoided, the dosage of the sodium hyaluronate can be reduced to be within 0.3 percent, and the phenomenon that the rheological property of the product is damaged due to overhigh dosage of the sodium hyaluronate is avoided. Moreover, compared with the traditional preservative system, the preservative system formed by reasonably proportioning the hexanediol and the p-hydroxyacetophenone can also greatly reduce the irritation of the product.

The skin barrier moisturizing repair cream disclosed by the invention has the advantages that a compact liquid crystal structure is formed together through the interaction among the oil moisturizing system, the emulsifying system and the lipoid compound, and a good moisturizing effect can be generated; meanwhile, due to the bionic lamellar liquid crystal structure design of the lipoid compound, physiological lipid can be effectively supplemented for the stratum corneum, the lipid barrier function of the stratum corneum of the skin is repaired, the percutaneous water loss is further reduced, and the water content of the stratum corneum is improved, so that the skin repairing effect is good. During the preparation process, the emulsion formed by the formula can obtain a stable final product through one phase transformation, can show good thixotropy (shear thinning), and can release the oil-phase component and the water-phase component simultaneously when being coated and used.

Drawings

FIG. 1 is a polarization micrograph of a lipid liquid crystal complex prepared according to one example.

FIG. 2 is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example III in example VI, FIG. 2A is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example IV, FIG. 2B is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example IV, and FIG. 2C is a graph showing the results of the chick embryo chorioallantoic membrane blood vessel test conducted on the skin barrier moisturizing cream of example V;

FIG. 3 is a graph showing the results of the percutaneous water loss test in example six.

Fig. 4 is a graph showing the results of the test for increasing the water content of the horny layer according to the sixth example.

Detailed Description

The first embodiment is as follows: preparation of lipid complexes

Weighing the components according to the following formula:

the preparation steps are as follows:

adding caprylic acid/capric acid triglyceride into ceramide 3, lauric acid, β -sitosterol and sodium polyacrylate respectively, stirring and heating to 90-100 ℃ for dissolution, keeping the temperature and stirring for 10-20 minutes to mix uniformly, and cooling to 75-85 ℃ to obtain an oil phase;

adding hydrogenated phosphatidylcholine, glycerol, disodium ethylene diamine tetraacetate and p-hydroxyacetophenone into purified water respectively, stirring and heating to 90-100 ℃ for dissolving, keeping the temperature and stirring for 10-20 minutes to uniformly mix, and cooling to 75-85 ℃ to obtain a water phase;

adding the oil phase into the water phase in a trickle shape at 75-85 ℃, and carrying out heat preservation, stirring and homogenizing for 10-20 minutes along the same direction to obtain an emulsified matrix;

and after emulsification is finished, stirring and cooling to below 45 ℃, then adding hexanediol and vitamin E into the emulsifying matrix, stirring uniformly, and continuously cooling to below 38 ℃ to obtain the lipoid liquid crystal compound.

Observing the prepared lipoid liquid crystal compound under a polarizing microscope, as shown in figure 1, molecules such as ceramide 3, lauric acid, β -sitosterol and the like are embedded in a lamellar liquid crystal body of the hydrogenated phosphatidylcholine together to form an orderly-arranged cross-shaped liquid crystal polarizing structure.

The liquid crystal is formed because the liquid crystal emulsifier and the auxiliary emulsifier form an interface film with ordered layers, compactness and viscoelasticity on an oil/water interface, the auxiliary emulsifier and the liquid crystal emulsifier which are properly proportioned are added, the ordered arrangement of the layers can be more favorably formed, and the hydrophilic group and water molecules form a gel network to be combined, so that the formula is more stable.

Example two: preparation of oily base

Weighing the components according to the following formula:

the preparation steps are as follows:

and (2) adding caprylic/capric triglyceride into squalane, shea butter, polydimethylsiloxane and olive oil unsaponifiable matter respectively, stirring and heating to 90-100 ℃, and uniformly mixing to obtain the oily matrix.

Example three: preparation of skin barrier moisturizing and repairing cream

Weighing the components according to the following formula:

the preparation steps are as follows:

adding acryloyl dimethyl ammonium taurate/VP copolymer, sodium polyacrylate and sodium hyaluronate into glycerol, and uniformly stirring to obtain a mixed solution;

taking an oily matrix, adding dibutyl hydroxy toluene, sucrose polystearate, cetearyl glucoside and cetearyl alcohol, stirring and heating to 90-100 ℃ for dissolving, keeping the temperature and stirring for 10-20 minutes, and then cooling to 75-85 ℃ to obtain an oil phase;

adding allantoin, p-hydroxyacetophenone and disodium ethylene diamine tetraacetate into purified water, stirring and heating to 90-100 ℃ for dissolving, preserving heat and stirring for 10-20 minutes, cooling to 75-85 ℃, adding the mixed solution, stirring for 10-20 minutes, homogenizing for 10-20 minutes, adding the oil phase, stirring and emulsifying for 10-20 minutes, homogenizing for 10-20 minutes, cooling to below 45 ℃, adding hexanediol, vitamin E and lipoid compound, stirring for 10-20 minutes, homogenizing for 10-20 minutes, and cooling to below 40 ℃, thus obtaining the skin barrier moisturizing and repairing cream.

Example four: preparation of skin barrier moisturizing and repairing cream

Weighing the components according to the following formula:

the preparation steps are as follows:

adding acryloyl dimethyl ammonium taurate/VP copolymer and sodium hyaluronate into glycerol, and uniformly stirring to obtain a mixed solution;

adding dibutyl hydroxy toluene, sucrose polystearate, sodium stearoyl lactylate, cetostearyl alcohol and glycerol stearate into an oily substrate, stirring and heating to 90-100 ℃ for dissolution, preserving heat and stirring for 10-20 minutes, and then cooling to 75-85 ℃ to obtain an oil phase;

Example five: preparation of traditional skin barrier moisturizing and repairing cream

Weighing the components according to the following formula:

the preparation steps are as follows:

adding sodium hyaluronate into glycerol, and stirring to obtain mixed solution;

taking neopentyl glycol diheptanoate, squalane, behenyl alcohol, C12-20 alkyl glucoside, cetearyl alcohol olive oleate, ceramide 3, cholesterol, octyldodecanol and dibutyl hydroxy toluene, stirring and heating to 90-100 ℃ for dissolving, continuing stirring for 10-20 minutes at a constant temperature, and then cooling to 75-85 ℃ to obtain an oil phase;

adding hydrogenated lecithin, glyceryl polyether-26, betaine, glyceryl polyacrylate, allantoin, p-hydroxyacetophenone and disodium ethylene diamine tetraacetate into purified water, stirring and heating to 90-100 ℃ for dissolution, continuing to keep the temperature and stir for 10-20 minutes, cooling to 75-85 ℃, then respectively adding the mixed solution and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, stirring for 10-20 minutes, homogenizing for 10-20 minutes, adding an oil phase, stirring and emulsifying for 10-20 minutes, homogenizing for 10-20 minutes, cooling to below 45 ℃, adding vitamin E and hexanediol, continuing to stir and emulsify for 10-20 minutes, homogenizing for 10-20 minutes, and cooling to below 40 ℃ to obtain the traditional skin barrier moisturizing and repairing cream.

Example six: performance testing

1. Irritation test

1) Chick embryo chorioallantoic membrane blood vessel test (CAM test)

The skin barrier moisturizing and repairing creams prepared in the third, fourth and fifth examples were respectively taken and subjected to a chick embryo chorioallantoic membrane blood vessel test to observe the blood vessel changes of the allantoic membrane after being exposed to the skin barrier moisturizing and repairing cream.

The test method comprises the following steps: 40ul of test samples are taken and directly dripped into a test ring on the surface of chorioallantoic membrane in the middle stage of fertilized chicken embryo with the embryo age of 10-14 days, and after the test ring is cultured for 30 minutes in an incubator at 37 ℃, the effect of the CAM blood vessel and the degree of each effect are recorded.

And (3) test results: as shown in fig. 2A, 2B and 2C, the skin barrier moisturizing and conditioning creams of examples three and four exhibited no irritation in the CAM test, were extremely mild in nature, and did not cause bleeding damage to the allantoic membrane vessels of chick embryo chorionic allantoic membrane; the skin barrier moisturizer of example five showed mild irritation in the CAM test, causing mild bleeding damage to the chorioallantoic vessels of chicken embryo hair.

And (4) judging the standard:

2) multiple skin irritation test

According to the method of "skin irritation/corrosion test" in the cosmetic safety specification 2015, the skin barrier moisturizing and repairing creams prepared in the third and fourth examples were respectively used, and rabbits were used as test animals to perform a skin irritation test.

The test method comprises the following steps: the test samples were applied to the skin of test animals (rabbits), wherein the sample groups were coated with the skin barrier moisturizing creams of example three and example four, respectively, and the blank group was coated with purified water, and the skin irritation degree of the test animals was observed and scored at regular time intervals.

And (4) judging the standard:

and (3) test results:

the test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment are non-irritant and have extremely mild properties.

3) Patch test on human skin

According to the method of the skin patch test for human body in the 2015 edition of the technical Specification for cosmetic safety, the skin barrier moisturizing and repairing creams prepared in the third and fourth examples are respectively taken to carry out the skin patch test for human body.

The test method comprises the following steps: selecting 30 subjects with an area of 50mm or less²And a spot tester with the depth not more than 1mm, wherein 0.02 g-0.025 g of test sample is smeared in the spot tester and then is pasted on the back of a subject. Wherein, the sample groups were coated with the skin barrier moisturizing cream of example three and example four, respectively, and the blank group was coated with purified water. After 24 hours, the plaque tester was removed, and the skin reaction of the subject was observed at 0.5 hour, 24 hours, and 48 hours after the removal, respectively, and the results of the skin reaction scale were recorded.

And (4) judging the standard: skin reaction grade standard of skin closed patch test

And (3) test results:

the test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment have no allergic reaction in the human skin patch test.

2. Percutaneous Water loss (TWEL) test

The skin barrier moisturizing and repairing creams prepared in the third, fourth and fifth examples were respectively used for a percutaneous water loss test with a human body as a parameter object (20 subjects per group).

The test method comprises the following steps: after the test site (inner side of front arm) was washed by the subject, the test site was marked with a test area (test area about 4 cm)²) And one arm is used as blank control, and the corresponding area of the other arm is smeared with the test sample. Under the condition of a constant temperature and humidity environment, after a subject enters a testing room and sits statically for 30 minutes, a percutaneous water loss measuring instrument is adopted to carry out initial reading on a testing area and a blank control area respectively; then, weighing a proper amount of test sample, uniformly coating the test sample in the test area, not processing the blank control area, reading the test area at regular intervals, and recording data.

And (3) test results:

test results show that after the skin barrier moisturizing and repairing cream provided by the third embodiment and the fourth embodiment of the invention is used, the percutaneous water loss can be rapidly reduced and can be maintained for a long time, and the skin barrier moisturizing and repairing cream is obviously superior to the traditional skin barrier moisturizing and repairing cream provided by the fifth embodiment.

3. Stratum corneum water content (MSC) lifting test

The skin barrier moisturizing and repairing creams prepared in the third, fourth and fifth examples were respectively used for stratum corneum water content increase test with human body as a parameter object (20 subjects per group).

The test method comprises the following steps: after the subject washes the test site (e.g., arm, forehead, etc.), the test site is circled to make a test area mark (test area about 4 cm)²) And selecting the area with the same size above the blank area as the blank control area. Under the condition of a constant temperature and humidity environment, after a subject enters a testing room and sits for 30 minutes, a stratum corneum water content tester is adopted to carry out initial reading on a testing area and a blank control area respectively; then, weighing a proper amount of test sample, uniformly coating the test sample in the test area, not processing the blank control area, reading the test area at regular intervals, and recording data.

And (3) test results:

test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment improve the moisture content of the stratum corneum by over 80 percent; the skin barrier moisturizing treatment cream of example five increased the moisture content of the stratum corneum by only about 60%.

4. Stability test

1) Accelerated test at 40 ℃

The test method comprises the following steps: the skin barrier moisturizing repair creams prepared in the third and fourth examples were placed in a 40 ℃ incubator, and examined for pH, uniformity, discoloration, and centrifugation within 3 months, and compared with the samples obtained at room temperature for 0 day.

And (3) test results: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples have good uniformity, no delamination after centrifugation and no obvious difference in pH value and color change compared with the samples obtained after 0 day at normal temperature under the condition of 3 months at 40 ℃.

2) Low temperature test at-15 deg.C

The test method comprises the following steps: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples were placed in an incubator at-15 ℃ to examine the pH value, uniformity, discoloration and centrifugation within 1 month, and compared with the samples obtained at room temperature for 0 day.

And (3) test results: the skin barrier moisturizing and repairing creams prepared in the third and fourth examples have good uniformity, no delamination after centrifugation and no obvious difference in pH value and color change compared with the samples obtained after 0 day at normal temperature under the condition of 1 month at-15 ℃.

3) Cold and hot cycle test

The test method comprises the following steps: the skin barrier moisturizing repair creams prepared in the third and fourth examples were placed in an incubator at 60 ℃ for 24 hours and then in an incubator at-15 ℃ for 24 hours, and the above steps were repeated 9 times to examine the properties, pH and consistency of the product after returning to room temperature, and compared with a sample at room temperature for 0 day.

And (3) test results: after 9 times of cold and hot cycles, the skin barrier moisturizing and repairing creams prepared in the third and fourth examples are uniform, fine and smooth in cream body, and have no obvious difference in pH value and consistency compared with the samples obtained after 0 day at normal temperature.

The stability test result shows that the skin barrier moisturizing repair cream prepared in the third example and the fourth example has good stability.

5. Test for Corrosion resistance

The skin barrier moisturizing skin creams prepared in example three and example four were respectively tested for antisepsis according to the microbial antisepsis efficacy test method of United states pharmacopoeia USP32<51 >. The test strains include: bacteria (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Burkholderia cepacia) and molds (Candida albicans, Aspergillus niger).

And (4) testing standard:

and (3) testing results:

the test results show that the bacterial mold of the antiseptic system of the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment does not increase after 14 days, and the reduction log value is larger than 2.

6. Risk substance testing

The skin barrier moisturizing repair creams prepared in example three and example four were subjected to the risk substance content test according to the risk substance test method of the cosmetic safety technical code 2015 edition.

And (3) testing results:

the test results show that the skin barrier moisturizing and repairing creams of the third embodiment and the fourth embodiment have the content of risk substances far smaller than the limit of national standards.

The comprehensive test results show that the skin barrier moisturizing and repairing cream prepared by the invention can effectively reduce the percutaneous water loss and improve the water content of the stratum corneum, has the effect of repairing the skin barrier, and has the advantages of mild property and low irritation.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. The skin barrier moisturizing and repairing cream is characterized by comprising the following components in percentage by weight:

and the balance purified water.

2. The skin barrier moisturizing and repairing cream as claimed in claim 1, wherein the oily base comprises the following components by weight percent:

3. the skin barrier moisturizing and repairing cream as claimed in claim 1, wherein the emulsifying system comprises the following components in percentage by weight:

30 to 40 percent of sucrose polystearate,

30 to 40 percent of cetearyl glucoside,

20 to 30 percent of cetearyl alcohol;

or the emulsifying system comprises the following components in percentage by weight:

4. the skin barrier moisturizing and repairing cream as claimed in claim 1, wherein the lipid compound comprises the following components by weight percent:

and the balance purified water;

5. The skin barrier moisturizing and repairing cream as claimed in claim 4, wherein the lipid compound further comprises the following components by weight percent:

wherein the thickening agent is selected from one or the combination of more than two of sodium polyacrylate, acryloyl dimethyl taurate, carbomer resin, ammonium taurine/behenyl alcohol polyether-25, methacrylate cross-linked polymer, sodium polyacrylate grafted starch and polyvinylpyrrolidone; the preservative is selected from one or more of hexanediol, p-hydroxyacetophenone, phenoxyethanol, hydroxyphenyl esters, pentanediol, caprylyl hydroximic acid and ethylhexyl glycerol; the antioxidant is selected from one or a combination of more than two of dibutyl hydroxy toluene, butyl hydroxy anisol and sodium sulfite; the chelating agent is one or more of ethylenediamine tetraacetic acid, disodium ethylenediamine tetraacetic acid, tetrasodium ethylenediamine tetraacetic acid, citric acid and sodium citrate.

6. The skin barrier moisturizing and repairing cream as claimed in claim 5, wherein the lipid compound comprises the following components by weight percent:

and the balance purified water.

7. The skin barrier moisturizing and repairing cream as claimed in one of claims 1 to 6, wherein the skin barrier moisturizing and repairing cream further comprises the following components by weight percent:

wherein the thickening agent is selected from one or the combination of more than two of acryloyl dimethyl ammonium taurate/vinyl pyrrolidone copolymer, sodium polyacrylate, acryloyl dimethyl sodium taurate, carbomer resin, methacrylate cross-linked polymer, sodium polyacrylate grafted starch and polyvinylpyrrolidone; the preservative is selected from one or more of hexanediol, p-hydroxyacetophenone, phenoxyethanol, hydroxyphenyl esters, pentanediol, caprylyl hydroximic acid and ethylhexyl glycerol; the antioxidant is selected from one or a combination of more than two of dibutyl hydroxy toluene, butyl hydroxy anisol and sodium sulfite; the chelating agent is one or more of ethylenediamine tetraacetic acid, disodium ethylenediamine tetraacetic acid, tetrasodium ethylenediamine tetraacetic acid, citric acid and sodium citrate.

8. The skin barrier moisturizing and repairing cream as claimed in claim 7, wherein the skin barrier moisturizing and repairing cream comprises the following components by weight percent:

and the balance purified water.

9. The skin barrier moisturizing and repairing cream as claimed in claim 7, wherein the skin barrier moisturizing and repairing cream comprises the following components by weight percent:

and the balance purified water.

10. A preparation method of a skin barrier moisturizing and repairing cream comprises the following steps: