CN110754566A - Fermented feed capable of promoting growth and development of bullfrogs and preparation method thereof - Google Patents
Fermented feed capable of promoting growth and development of bullfrogs and preparation method thereof Download PDFInfo
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- CN110754566A CN110754566A CN201911196522.2A CN201911196522A CN110754566A CN 110754566 A CN110754566 A CN 110754566A CN 201911196522 A CN201911196522 A CN 201911196522A CN 110754566 A CN110754566 A CN 110754566A
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Abstract
The invention discloses a fermented feed capable of promoting the growth and development of bullfrogs, which comprises 15-30 parts of fish meal, 5-10 parts of meat and bone meal, 10-20 parts of chicken meal, 17-30 parts of peanut meal, 5-12 parts of corn protein powder, 16-30 parts of flour, 0.5-1 part of vitamin premixed feed for the bullfrogs, 0.5-1 part of multi-mineral premixed feed for the bullfrogs and 0.4-0.8 part of bile acid in parts by weight, and also comprises the following fermented components in parts by weight: 1-3Kg of yeast, 1-2Kg of lactic acid composite bacteria, 1-2.5Kg of spore composite bacteria and 0.5-1Kg of complex enzyme. Also discloses a preparation method of the fermented feed capable of promoting the growth and development of bullfrogs. The invention has simple and convenient raw material acquisition and simple and feasible manufacturing method, can effectively solve the problems of heavy intestinal tract burden, unhealthy intestinal tract, weak disease resistance and the like caused by slow growth rate and low digestion rate of the bullfrogs in a high-density breeding mode, can obviously improve the specific growth rate of the bullfrogs during the breeding period, reduce the feed coefficient, reduce the dosage and greatly improve the breeding benefit.
Description
Technical Field
The invention relates to the field of special feed for bullfrogs, in particular to fermented feed capable of promoting growth and development of bullfrogs and a preparation method thereof.
Background
Bullfrog is the most famous edible frog in the world, and is named because its male ringing sound is like a cow. The bullfrog meat is white and tender, and has delicious taste and rich nutrition. Compared with chicken, duck, pig, cattle, sheep, etc., it is a nutritious health food with high protein, low fat, low calorie, and low cholesterol. Meanwhile, the nutrient also contains various micronutrients such as calcium, phosphorus, iron, thiamine, riboflavin, nicotinic acid and the like. According to modern traditional Chinese medicine, the bullfrog serving as a health food has the nourishing effects of tonifying yang, promoting diuresis, invigorating stomach, tonifying brain, tonifying deficiency, relieving cough and the like, can relieve hyperacidity, clear away heat and toxic materials, and has great effects on recovering physical fitness of people with physical weakness. The skin, skeleton and internal organs of bullfrog contain abundant collagen, and the bullfrog is a good food additive and dietary therapy tonic. In addition, from the culture perspective, the bullfrog culture technology has low requirement, the culture threshold is low, the feed coefficient of the bullfrog is low, the culture benefit is good, and the bullfrog is widely pursued by farmers. The existing bullfrog breeding industry develops rapidly, and breeding areas are mainly focused on Yuedong and Minnan.
However, the bullfrog breeding has some problems, and in the existing bullfrog breeding mode, the high-density breeding mode of deep water or semi-deep water is mainly used, so that the high-density breeding brings high income to farmers, and simultaneously brings the following problems:
1. the activity space of the bullfrog is compressed, and the growth speed of the bullfrog is reduced;
2. the high-density bred bullfrogs have poor living environment, low body resistance and low digestion level, are greedy to eat, and are infected by intestinal inflammation, so that other internal organs are affected to cause death;
3. the high-density culture causes the water quality to be poor, pathogens such as bacteria and the like are easy to breed, so that the bullfrogs are easy to catch diseases, and the dosage is very high.
The bullfrog feed appearing in the market at present mainly takes a bullfrog complete formula feed as a main material, and a functional feed is not reported. The common complete formula feed can meet the nutritional requirements of bullfrogs in all stages, but cannot solve the problems of low digestion level, slow growth speed and high dosage of the bullfrogs under high-density culture. This also restricts the further development of the bullfrog breeding industry.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a fermented feed capable of promoting the growth and development of bullfrogs and a preparation method thereof, aiming at the problems of low digestion and utilization rate, slow growth speed, high dosage and the like of bullfrogs, and the fermented feed can improve the absorption efficiency of bullfrogs, enhance the immunity of frogs, improve the water quality and reduce the dosage.
In order to achieve the purpose, the invention provides the following technical scheme:
a fermented feed capable of promoting growth and development of bullfrogs comprises the following components: fish meal, meat and bone meal, chicken meal, peanut meal, corn protein powder, flour, vitamin premixed feed for frogs, multi-mineral premixed feed for frogs and bile acid.
As a preferred technical scheme, the feed comprises, by weight, 15-30 parts of fish meal, 5-10 parts of meat and bone meal, 10-20 parts of chicken meal, 17-30 parts of peanut meal, 5-12 parts of corn protein powder, 16-30 parts of flour, 0.5-1 part of vitamin premixed feed for frogs, 0.5-1 part of multi-mineral premixed feed for frogs and 0.4-0.8 part of bile acid.
As a preferred technical scheme, the fermentation medium further comprises the following fermentation components: yeast, lactic acid composite bacteria, spore composite bacteria and composite enzyme.
As a preferred technical scheme, the weight of each fermentation component is as follows: the weight of each fermentation component is as follows: 1-3Kg of yeast, 1-2Kg of lactic acid composite bacteria, 1-2.5Kg of spore composite bacteria and 0.5-1Kg of complex enzyme.
As a preferred technical scheme, the lactic acid complex bacteria comprise pediococcus acidilactici and lactobacillus acidophilus, wherein the content of the pediococcus acidilactici is not lower than 500 hundred million CFU/g, and the content of the lactobacillus acidophilus is not lower than 500 hundred million CFU/g.
As a preferred technical scheme, the spore compound bacteria comprise bacillus subtilis and bacillus licheniformis, wherein the content of the bacillus subtilis is not lower than 1000 hundred million CFU/g, and the content of the bacillus licheniformis is not lower than 500 hundred million CFU/g.
As a preferable technical scheme, the complex enzyme comprises 10000U/g acid protease, 5000U/g neutral protease, 5000U/g xylanase, 8000U/g cellulase, 2000U/g glucose oxidase and 5000U/g pectinase.
As a preferred technical scheme, the fish meal is seawater fish meal.
As a preferable technical scheme, the fermented feed capable of promoting the growth and development of bullfrogs comprises the following components in parts by weight: 20 parts of seawater fish meal, 8 parts of meat and bone meal, 20 parts of chicken meal, 22 parts of peanut meal, 8 parts of corn protein powder, 20 parts of flour, 0.7 part of vitamin premixed feed for frogs, 0.7 part of multi-mineral premixed feed for frogs and 0.6 part of bile acid;
the fermentation liquid also comprises the following fermentation components in parts by weight: 3Kg of yeast, 1.8Kg of lactic acid composite bacteria, 2.2Kg of spore composite bacteria and 0.6Kg of complex enzyme.
The invention also provides a preparation method of the fermented feed capable of promoting the growth and development of bullfrogs, which specifically comprises the following steps:
(1) granulating
Mixing the above components except for the fermented component, and making into granule with particle size of 1.5-12 mm;
(2) preparation before fermentation
Dissolving the fermentation components in water to form a compound bacterial liquid;
(3) fermentation of
Atomizing and spraying the composite bacterial liquid onto the screened granules, stirring uniformly, carrying out aerobic fermentation for 15-30h, bagging, and carrying out anaerobic fermentation for 5-10 days to obtain the composite bacterial liquid.
As a preferable technical scheme, the granules are prepared by puffing and granulating in the step (1);
carrying out aerobic fermentation in a fermentation box; after the anaerobic fermentation is finished, the fermented feed is subpackaged into plastic film bags with one-way valves, and the bags are sealed so as to be convenient to store.
Compared with the prior art, the invention has the following advantages:
a. while meeting the daily nutritional basic requirements of bullfrogs, the anti-nutritional factors in the feed are greatly reduced through a fermentation process, the absorption efficiency of the bullfrogs can be improved, the growth rate is accelerated, and meanwhile, the feed has a large amount of beneficial bacteria and fermentation metabolites, so that the immunity and the antibacterial capability of the frogs can be enhanced, and the dosage is reduced;
b. compared with the common complete bullfrog compound feed, the invention uses the viable bacteria for solid fermentation, greatly reduces the anti-nutritional factors in the feed, simultaneously degrades some macromolecular substances which can not be digested and absorbed by the bullfrog, can improve the digestion and utilization rate of the bullfrog on the feed, and quickens the growth rate of the bullfrog;
c. compared with the common complete bullfrog compound feed, various active probiotics contained in the feed can form probiotic groups in the bullfrog intestinal tract, improve the intestinal health level, acidify the intestinal tract and resist the invasion of harmful bacteria; the fermentation metabolite contains various oligosaccharides, small peptides, vitamins and unknown growth factors, so that the immunity level of bullfrogs can be improved, the disease resistance is improved, and the dosage is reduced;
d. compared with the common complete bullfrog compound feed, the compound feed has a large amount of live bacteria, so that the microorganism on the bullfrog excrement and the feed residual bait is greatly increased, the decomposition and the conversion of the excrement and the feed residual bait are accelerated, the water quality is improved, the opportunity of infecting germs by the bullfrog is reduced to a greater extent, and a good environment is provided for the growth and the development of the bullfrog;
e. the fermented feed disclosed by the invention is stable in raw material source, relatively low in cost and simple in preparation process, and can meet the nutritional requirements of bullfrog in each growth stage;
f. the selected strains are easy to obtain, the cost is low, the fermentation effect is satisfactory, the granulated feed can be fully degraded, macromolecular substances and anti-nutritional factors in feed raw materials are reduced to a greater extent, and a large amount of beneficial metabolites are generated.
Drawings
FIG. 1 is a schematic representation of the digestion of the intragastric feed in each group three hours after experiment 1;
FIG. 2 is a schematic representation of the digestion of the intragastric feed in each group six hours after test example 1;
FIG. 3 is a schematic diagram showing the digestion of the intragastric feed in each group nine hours after experiment 1;
FIG. 4 is a schematic view of the intestinal section of bullfrog in the control group in Experimental example 2;
FIG. 5 is a schematic view of sliced intestinal tracts of bullfrog in Experimental group of Experimental example 2.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The reagents, equipment and methods adopted by the invention are all reagents, equipment and methods which are conventionally and commercially available in the technical field and are conventionally used in the technical field.
Example 1
A fermented feed capable of promoting the growth and development of bullfrogs comprises, by weight, 20 parts of seawater fish meal, 8 parts of meat and bone meal, 20 parts of chicken meal, 22 parts of peanut meal, 8 parts of corn protein powder, 20 parts of flour, 0.7 part of vitamin premixed feed for bullfrogs, 0.7 part of multi-mineral premixed feed for bullfrogs and 0.6 part of bile acid;
by weight, the fermented food further comprises the following fermentation components: 3Kg of yeast, 1.8Kg of lactic acid composite bacteria, 2.2Kg of spore composite bacteria and 0.6Kg of complex enzyme.
Vitamin premix feeds for frogs and multimineral premix feeds for frogs used in the present examples are known materials (see Schuhmacher, Germany, et al, Aquaculture,1997, 151: 15-28), the Netherlands).
The preparation method comprises the following steps:
(1) granulating
Mixing seawater fish powder, meat and bone powder, chicken powder, peanut meal, corn protein powder, flour, frog vitamin premixed feed, frog multi-mineral premixed feed, and bile acid, puffing, and granulating to obtain granule with particle size of 1.5-12 mm;
(2) preparation before fermentation
Dissolving 3Kg of yeast, 1.8Kg of lactic acid composite bacteria, 2.2Kg of spore composite bacteria and 0.6Kg of complex enzyme into 350Kg of water to form a composite bacterial liquid;
(3) fermentation of
Atomizing and spraying the composite bacterial liquid onto 800Kg of granules, stirring uniformly, carrying out aerobic solid fermentation in a fermentation box, fermenting for 15-30h according to the ambient temperature, loading into a ton bag after the fermentation is finished, carrying out anaerobic fermentation in the ton bag for 5-10 days according to the ambient temperature, subpackaging into plastic bags with one-way valves after the fermentation is finished, and sealing to obtain the feed additive.
Example 2
A fermented feed capable of promoting the growth and development of bullfrogs comprises, by weight, 15 parts of seawater fish meal, 10 parts of meat and bone meal, 10 parts of chicken meal, 30 parts of peanut meal, 5 parts of corn protein powder, 30 parts of flour, 0.5 part of vitamin premixed feed for bullfrogs, 0.5 part of multi-mineral premixed feed for bullfrogs and 0.8 part of bile acid;
also comprises a fermentation component: 3Kg of yeast, 1Kg of lactic acid composite bacteria, 2.5Kg of spore composite bacteria and 0.5Kg of complex enzyme.
Vitamin premix feeds for frogs and multimineral premix feeds for frogs used in the present examples are known materials (see Schuhmacher, Germany, et al, Aquaculture,1997, 151: 15-28), the Netherlands).
The preparation method is the same as that of example 2, and is not repeated herein.
Example 3
A fermented feed capable of promoting growth and development of bullfrogs comprises, by weight, 30 parts of seawater fish meal, 5 parts of meat and bone meal, 20 parts of chicken meal, 17 parts of peanut meal, 12 parts of corn protein powder, 16 parts of flour, 1 part of vitamin premixed feed for bullfrogs, 1 part of multi-mineral premixed feed for bullfrogs and 0.4 part of bile acid;
also comprises a fermentation component: 1Kg of yeast, 2Kg of lactic acid composite bacteria, 1Kg of spore composite bacteria and 1Kg of complex enzyme.
Vitamin premix feeds for frogs and multimineral premix feeds for frogs used in the present examples are known materials (see Schuhmacher, Germany, et al, Aquaculture,1997, 151: 15-28), the Netherlands).
The preparation method is the same as that of example 2, and is not repeated herein.
Experimental example 1
Purpose of the experiment: the digestion of the feed in the stomach within 9 hours was compared by feeding the common complete bullfrog compound feed and the fermented feed prepared in example 1.
The experimental method comprises the following steps:
26 bullfrogs of the same variety are taken and divided into two groups, and each group comprises 13 bullfrogs. One group was fed with the fermented feed prepared in example 1 as an experimental group; feeding the other group with common complete bullfrog compound feed as control group; the experimental group and the control group have the same feeding environment and feeding condition.
After feeding for 3h, 5 bullfrogs of the experimental group and the control group are respectively taken and dissected, as shown in figure 1, compared with the bullfrogs of the control group, most of the bullfrog gastric feed of the experimental group is minced, the granular feed accounts for a few, while most of the bullfrog gastric feed of the control group is obviously granular, the polymerization degree is higher, and only a few feeds are minced.
After feeding for 6h, 5 bullfrogs in the experimental group and the control group were each dissected, and as shown in fig. 2, the intragastric feed of the bullfrogs in the experimental group was all minced and no granular feed was present, while the intragastric feed of the bullfrogs in the control group was still granular and distinct, but had loose polymerization degree and few minced feeds.
After feeding for 9h, 3 bullfrogs in the experimental group and the control group were each dissected, and as shown in fig. 3, most of the bullfrog gastric feed in the experimental group was emptied and only a small amount of minced feed was present, while most of the bullfrog gastric feed in the control group was minced and a small amount of granulated feed was present, compared to the bullfrogs in the control group.
Therefore, the fermented feed prepared in example 1 can obviously promote digestion and absorption of bullfrog intestines and stomach.
Experimental example 2
Purpose of the experiment: the intestinal development of bullfrog was compared by feeding the general complete bullfrog formula feed and the fermented feed prepared in example 1.
The experimental method comprises the following steps:
30 bullfrogs of the same variety are taken and divided into two groups, and each group comprises 15 bullfrogs. One group was fed with a common complete bullfrog mixed feed and added with 25% of the fermented feed prepared in example 1 as an experimental group; feeding the other group with common complete bullfrog compound feed as control group; the experimental group and the control group have the same feeding environment and feeding condition. After feeding for 30 days, the bullfrog in the experimental group and the bullfrog in the control group are respectively sampled and dissected to prepare intestinal slices.
Intestinal sections of bullfrog in the control group are shown in FIG. 4.
Intestinal sections of experimental bullfrogs are shown in FIG. 5.
The data statistics are as follows:
as can be seen from the slicing data, the intestinal wall thickness, the number of goblet cells, the number of villi and the ratio of villi area of the bullfrogs fed by using 25 percent of the fermented feed of the example 1 are all superior to those of bullfrogs fed by only using the full-price compound feed of common bullfrogs.
Experimental example 3
The experimental site: shantou Shanshi Denghai district of Guangdong province |
The specification of the culture pond is as follows: area of 160m26, 6 |
The number of the bullfrogs is as follows: about 50000 pieces in total |
Putting the bullfrog into the container: eating No. 3 material specification |
Control group
The bullfrog is only fed by using the complete formula feed, and the breeding conditions of the bullfrog are as follows:
no. 1 culture pond: the average initial weight is 50.67g, the cultivation time is 34 days, the average final weight is 81.9g, the specific growth rate is 1.41 percent, and the feed coefficient is 0.56.
No. 2 culture pond: the average initial weight is 61.5g, the cultivation time is 34 days, the average final weight is 85.86g, the specific growth rate is 0.98%, and the feed coefficient is 0.89.
No. 3 culture pond: the average initial weight is 52.22g, the cultivation time is 34 days, the average final weight is 87.36g, the specific growth rate is 1.51 percent, and the feed coefficient is 0.50.
Experimental group
The fermented feed of example 1 was fed in a mixed manner at a concentration of 25%, and the bullfrog was bred as follows:
no. 4 culture pond: the average initial weight is 36.72g, the cultivation time is 34 days, the average final weight is 79.56g, the specific growth rate is 2.27 percent, and the feed coefficient is 0.52.
The No. 5 culture pond has average initial weight of 51.58g, culture time of 34 days, average final weight of 92.88g, specific growth rate of 1.73 percent and feed coefficient of 0.54.
The No. 6 culture pond has an average initial weight of 42.49g, a culture time of 34 days, an average final weight of 74.12g, a specific growth rate of 1.64 percent and a feed coefficient of 0.70.
The average specific growth rate of bullfrogs in the control group is 1.30%, and the average specific growth rate of bullfrogs in the experimental group is 1.88%.
The average feed factor for the control group was 0.65 and the average feed factor for the experimental group was 0.58.
From the 34-day growth data, it can be seen that the experimental group combined with the bullfrog fed with 25% of the fermented feed of example 1 has a specific growth rate and a feed coefficient superior to those of the bullfrog fed with the conventional full-value compound feed alone. Meanwhile, various medicines are required to be mixed in the complete formula feed, and the fermented feed in the example 1 is used without any medicine, so that the feed can be directly fed, and the dosage is reduced by about 25 percent in the whole process.
The fermented feed provided by the invention has the advantages of simple and convenient raw material acquisition and simple and feasible manufacturing method, and can effectively solve the problems of heavy intestinal tract burden, unhealthy intestinal tract, weak disease resistance and the like caused by slow growth rate and low digestion rate of bullfrogs in a high-density breeding mode. Experimental examples 1-3 show that the specific growth rate of bullfrogs can be obviously improved, the feed coefficient can be reduced, the dosage can be reduced, and the breeding benefit can be greatly improved by using the fermented feed of the invention.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and their concepts should be considered to be equivalent or modified within the technical scope of the present invention.
Claims (10)
1. A fermented feed capable of promoting growth and development of bullfrogs is characterized by comprising the following components: fish meal, meat and bone meal, chicken meal, peanut meal, corn protein powder, flour, vitamin premixed feed for frogs, multi-mineral premixed feed for frogs and bile acid.
2. The fermented feed capable of promoting bullfrog growth and development according to claim 1, is characterized in that the weight portions of the components are 15-30 portions of fish meal, 5-10 portions of meat and bone meal, 10-20 portions of chicken meal, 17-30 portions of peanut meal, 5-12 portions of corn protein powder, 16-30 portions of flour, 0.5-1 portion of vitamin premixed feed for bullfrog, 0.5-1 portion of multi-mineral premixed feed for bullfrog and 0.4-0.8 portion of bile acid.
3. The fermented feed capable of promoting the growth and development of bullfrogs according to claim 1, further comprising the following fermentation components: yeast, lactic acid composite bacteria, spore composite bacteria and composite enzyme.
4. The fermented feed capable of promoting the growth and development of bullfrogs as claimed in claim 3, wherein the weight of each fermented component is as follows: 1-3Kg of yeast, 1-2Kg of lactic acid composite bacteria, 1-2.5Kg of spore composite bacteria and 0.5-1Kg of complex enzyme.
5. The fermented feed capable of promoting the growth and development of bullfrog according to claim 3, characterized in that the lactic acid complex bacteria comprise Pediococcus acidilactici and Lactobacillus acidophilus, the content of Pediococcus acidilactici is not less than 500 hundred million CFU/g, and the content of Lactobacillus acidophilus is not less than 500 hundred million CFU/g.
6. The fermented feed capable of promoting the growth and development of bullfrog according to claim 3, characterized in that the spore complex bacteria comprise bacillus subtilis and bacillus licheniformis, the content of the bacillus subtilis is not lower than 1000 hundred million CFU/g, and the content of the bacillus licheniformis is not lower than 500 hundred million CFU/g.
7. The fermented feed capable of promoting the growth and development of bullfrogs according to claim 3, wherein the complex enzyme comprises 10000U/g of acid protease, 5000U/g of neutral protease, 5000U/g of xylanase, 8000U/g of cellulase, 2000U/g of glucose oxidase and 5000U/g of pectinase.
8. The fermented feed capable of promoting the growth and development of bullfrogs according to claim 1, which is characterized by comprising the following components in parts by weight: 20 parts of seawater fish meal, 8 parts of meat and bone meal, 20 parts of chicken meal, 22 parts of peanut meal, 8 parts of corn protein powder, 20 parts of flour, 0.7 part of vitamin premixed feed for frogs, 0.7 part of multi-mineral premixed feed for frogs and 0.6 part of bile acid;
the fermentation liquid also comprises the following fermentation components in parts by weight: 3Kg of yeast, 1.8Kg of lactic acid composite bacteria, 2.2Kg of spore composite bacteria and 0.6Kg of complex enzyme.
9. The method for preparing a fermented feed for promoting the growth and development of bullfrogs according to any one of claims 1 to 8, comprising the steps of:
(1) granulating
Mixing the other components except the fermented component, and making into granule with particle size of 1.5-12 mm;
(2) preparation before fermentation
Dissolving the fermentation components in water to form a compound bacterial liquid;
(3) fermentation of
Atomizing and spraying the composite bacterial liquid onto the screened granules, stirring uniformly, carrying out aerobic fermentation for 15-30h, bagging, and carrying out anaerobic fermentation for 5-10 days to obtain the composite bacterial liquid.
10. The method for preparing fermented feed capable of promoting growth and development of bullfrogs according to claim 9, wherein the granules are prepared by puffing and granulating in the step (1); carrying out aerobic fermentation in a fermentation box; after the anaerobic fermentation is finished, subpackaging the fermented feed into plastic film bags with one-way valves, and sealing.
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CN113598272A (en) * | 2021-08-16 | 2021-11-05 | 武汉轻工大学 | Disease-resistant growth-promoting compound feed for adult rana nigromaculata and preparation method thereof |
CN114468144A (en) * | 2020-10-26 | 2022-05-13 | 河北科星药业有限公司 | Feed additive for preventing bullfrog gastroenteritis and preparation method and application thereof |
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CN105495044A (en) * | 2015-12-19 | 2016-04-20 | 界首市众鑫科技服务有限公司 | Selenium-rich bullfrog-tadpole feed |
CN108077567A (en) * | 2017-12-26 | 2018-05-29 | 湖州海皇生物科技有限公司 | A kind of bullfrog mixed feed with protection liver function and preparation method thereof |
CN109717307A (en) * | 2019-02-19 | 2019-05-07 | 江门市澳华生物科技有限公司 | A kind of Thailand's frog feed |
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CN102160599A (en) * | 2011-03-07 | 2011-08-24 | 顾建洪 | Biologically-fermented complete compound feed |
CN105495044A (en) * | 2015-12-19 | 2016-04-20 | 界首市众鑫科技服务有限公司 | Selenium-rich bullfrog-tadpole feed |
CN108077567A (en) * | 2017-12-26 | 2018-05-29 | 湖州海皇生物科技有限公司 | A kind of bullfrog mixed feed with protection liver function and preparation method thereof |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN114468144A (en) * | 2020-10-26 | 2022-05-13 | 河北科星药业有限公司 | Feed additive for preventing bullfrog gastroenteritis and preparation method and application thereof |
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