CN110731282A - sprinkling summer-heat-relieving bacteria - Google Patents
sprinkling summer-heat-relieving bacteria Download PDFInfo
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- CN110731282A CN110731282A CN201911174610.2A CN201911174610A CN110731282A CN 110731282 A CN110731282 A CN 110731282A CN 201911174610 A CN201911174610 A CN 201911174610A CN 110731282 A CN110731282 A CN 110731282A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/30—Culture of aquatic animals of sponges, sea urchins or sea cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides splashing type summer-heat relieving bacteria, which are characterized by being prepared by uniformly mixing, crushing and filtering a component , a component two and a component three, wherein the component is a composite strain synthetic material, the component two is a mixture of leguminous plants and zingiberaceae plants, the component three is a mixture of herbaceous plants, the mixture of herbaceous plants is more than selected from mint, houttuynia cordata, cortex lycii radicis, plantain herb and mulberry leaves, and the mass ratio of the component , the component two and the component three is 6-10:1-3: 1.
Description
Technical Field
The invention relates to the field of aquatic animal culture, in particular to splashing type summer-heat-relieving bacteria.
Background
The heat energy brought by the global temperature rise has led to the frequent occurrence of disasters such as large-scale or even ultra-large-scale typhoons, hurricanes, rainstorms, intense heat and the like. Annual climatic disasters of aquaculture are increasing. In recent years, the breeding disasters of sea cucumbers, river crabs and the like caused by severe summer heat bring huge economic losses to breeders.
Aiming at the problem that how to improve the heatstroke resistance and stress resistance of aquatic animals in a severe heatstroke environment, namely how to promote the appetite of the aquatic animals in a high water temperature environment, strengthen the physique and improve the heatstroke resistance, is needed to be solved at present.
Disclosure of Invention
The invention aims to overcome the defects and provides herbal microecologics which are splashed to a water body, and the summer-heat-relieving bacteria can obviously improve the appetite of aquatic animals in a high-water-temperature environment, enhance the physique and improve the summer-heat resistance by improving the water body by using beneficial bacteria and effectively dissolving herbal plant particles in the water body and playing a role in the permeation of the aquatic animals.
The invention provides splashing type summer-heat relieving bacteria, which is characterized by being prepared by uniformly mixing, crushing and filtering the following components , and III by a screen mesh:
wherein the component is a composite strain synthetic material;
the second component is mixture of leguminous plant and zingiberaceae plant;
the third component is a herbaceous plant mixture;
the herbal mixture is selected from more than of herba Menthae, herba Houttuyniae, cortex Lycii, herba plantaginis, and folium Mori;
the mass ratio of the component to the component II to the component III is 6-10:1-3: 1.
, the splashing summer-heat-relieving bacteria is characterized in that the component is formed by mixing compound strains, aerobic bacteria and zeolite powder;
the mass part ratio of the composite strain, the aerobic bacteria and the zeolite powder is 20-35: 1: 50-80 parts;
wherein the composite strain is a fermentation product of EM strain.
The EM strain in the invention can be a mixture of more than of rhodopseudomonas palustris, lactobacillus acidophilus, brewer's yeast, aspergillus actinomycete and bacillus subtilis.
, the splashing summer-heat-relieving bacteria are characterized in that the preparation method of the composite bacteria is that EM bacteria and saline water are poured into a fermentation tank for fermentation according to the proportion of 8-20: 1, the temperature is kept at 25-30 ℃, and anaerobic fermentation culture is carried out for 4-10 days to obtain the composite bacteria.
, the sprinkling summer-heat-relieving bacteria is characterized in that the saline is sodium chloride and/or potassium chloride solution with the mass concentration of 1-5%.
, the splashing summer-heat-relieving bacteria are characterized in that the mass ratio of the leguminous plants to the zingiberaceae plants is 1: 2-5.
the sprinkling summer-heat removing bacteria is characterized in that the leguminous plants are selected from radix sophorae flavescentis.
the sprinkling summer-heat-relieving bacteria is characterized in that the dosages of the herbaceous plants in the herbaceous plant mixture are equal.
, the sprinkling type summer-heat removing bacteria provided by the invention are further characterized in that according to the target dosage, the water is diluted by 10-20 times of pool water and then evenly sprinkled in the target pool.
, the splashing summer-heat-relieving bacteria are also characterized in that the bacteria are used in high-temperature seasons to help aquatic animals relieve summer heat and reduce heat and improve anti-stress capability;
and/or
The microbial agent is used for inhibiting the colonization and invasion of pathogenic bacteria in a high-temperature water body and limiting the outbreak of high-temperature pathogenic factors;
and/or
The functions of regulating fat metabolism, resisting genetic material DNA variation and repairing cell damage;
and/or
The product has effects in resisting free radical injury, reducing lipid peroxide, inhibiting aquatic animal viscera necrosis, and protecting health;
and/or
Is used for promoting secretion of endogenous digestive enzyme, improving appetite of aquatic animals, and enhancing digestion and absorption of feed.
, the sprinkling type summer-heat-relieving bacteria are characterized in that when the bacteria are used for improving the anti-stress capability, 500 g/mu are used for the first time, 50-100 g/mu are used for the first time, times are sprinkled every 5-7 days, and 1-3 times of the bacteria are sprinkled twice when the water quality is deteriorated;
when used for raising seedlings, 1 to 5 grams of water is sprinkled on each cubic meter of water, and times of sprinkling are sprinkled every 2 to 3 days.
The invention has the following functions and effects:
the invention is suitable for all seawater and fresh water fish, shrimp, crab, shellfish, sea cucumber breeding ponds and culture ponds (including mixed culture ponds), and is particularly suitable for culturing aged water bodies which have more sludge, generate 'white turbid water', 'rust water', 'black smelly water', 'old green water' and cause poor appetite, malnutrition, abnormal body color and slow growth of aquatic animals for many years.
The summer-heat relieving bacteria of the invention are developed for solving the great harm of summer heat and temperature to the aquaculture industry. The product is prepared by screening genuine materials capable of improving the heatstroke and stress resistance of aquatic animals in natural herbaceous plants, culturing in liquid or solid state fermentation, and adding special herbaceous plant adjuvants.
The heatstroke-relieving bacterium promotes the aquatic animals to absorb and utilize functional substances such as heatstroke relieving, heat reduction, stress resistance and the like in the herbaceous plants put in the water body by regulating the structure of the dominant microecological flora of the aquaculture pond, so that the high tolerance of the aquatic animals to the water temperature of the pond in summer severe summer is enhanced.
The invention provides herbal microecologics which are splashed to a water body, and the prepared herbal microecologics can obviously improve the appetite of aquatic animals in a high water temperature environment, enhance physique and improve the heatstroke resistance by improving the water body by using beneficial bacteria and effectively dissolving herbal plant particles in the water body and permeating the aquatic animals.
Drawings
FIG. 1 is a graph showing the comparison of ammonia nitrogen content in Experimental example 1;
FIG. 2 is a graph showing the comparison of nitrite contents in Experimental example 1;
FIG. 3 is a graph showing the comparison of ammonia nitrogen content in Experimental example 2;
FIG. 4 is a graph showing the nitrite content comparison in Experimental example 2.
Detailed Description
Examples 1,
The sprinkling type summer-heat removing bacteria No. 1 product provided by the embodiment 1 is prepared by the following steps:
1. preparing a composite strain: mixing EM strain (8-12 parts of Rhodopseudomonas palustris, 4-6 parts of Lactobacillus acidophilus, 1-3 parts of brewer's yeast, 1-2 parts of Actinomyces Monascus and 1-3 parts of Bacillus subtilis), wherein the content of the strain is 30 × 108cfu/ml or more; lactobacillus acidophilus in lactobacillus with strain content of 5 × 108cfu/ml or more; the Saccharomyces cerevisiae has strain content of 20 × 108cfu/ml or more; the content of actinomycete in filamentous fungi is 5 × 106cfu/ml or more) with 1% light saline according to 10:1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and performing anaerobic fermentation culture for 10 days to prepare the composite strain for later use.
2. Taking 30% of powder bacillus natto, 1% of compound bacteria (prepared in step ) and 69% of zeolite powder, mixing, drying, and preparing into synthetic material for later use.
3. Taking the sophora flavescens powder and the ginger powder, compounding and mixing according to the proportion of 1:3, and fully and uniformly stirring for later use.
4. Compounding and mixing mint, houttuynia cordata, cortex lycii radicis, plantain and mulberry leaves according to the proportion of 1:1:1:1:1, and fully and uniformly stirring for later use.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 7:2:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
Examples 2,
The splashing type summer-heat relieving bacteria 2# product provided by the embodiment 2 is prepared by the following method:
1. preparing a composite strain: taking EM strain and 5% light saline according to the ratio of 8: 1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and performing anaerobic fermentation culture for 8 days to prepare the composite strain for later use.
2. Taking 28% of powder bacillus natto, 1% of compound bacteria (prepared in step ) and 71% of zeolite powder, mixing, drying, and preparing into synthetic material for later use.
3. Mixing radix Sophorae Flavescentis powder and rhizoma Zingiberis recens powder at a ratio of 1:2, and stirring.
4. Compounding and mixing mint, houttuynia cordata, cortex lycii radicis, plantain and mulberry leaves according to the proportion of 1:1:1:1:1, and fully and uniformly stirring for later use.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 5:2:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
Examples 3,
The splashing type summer-heat relieving bacteria 3# product provided by the embodiment 3 is prepared by the following method:
1. preparing a composite strain: taking EM strain and 1% light saline according to the ratio of 20: 1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and carrying out anaerobic fermentation culture for 4 days to prepare the composite strain for later use.
2. Taking 20% of powder bacillus natto, 1% of compound bacteria (prepared in step ) and 79% of zeolite powder, mixing, drying, and preparing into synthetic material for later use.
3. Taking the sophora flavescens powder and the ginger powder, compounding and mixing according to the proportion of 1:5, and fully and uniformly stirring for later use.
4. Compounding and mixing mint, houttuynia cordata, cortex lycii radicis, plantain and mulberry leaves according to the proportion of 1:1:1:1:1, and fully and uniformly stirring for later use.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 7:5:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
Examples 4,
The splashing type summer-heat relieving bacteria 4# product provided by the embodiment 4 is prepared by the following method:
1. preparing a composite strain: taking EM strain and 5% light saline according to the ratio of 8: 1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and carrying out anaerobic fermentation culture for 5 days to prepare the composite strain for later use.
2. Taking 35% of powder bacillus natto, 1% of compound bacteria (prepared in step ) and 64% of zeolite powder, mixing, drying, and preparing into synthetic material for later use.
3. Taking the sophora flavescens powder and the ginger powder, compounding and mixing according to the proportion of 1:3, and fully and uniformly stirring for later use.
4. Compounding and mixing mint, houttuynia cordata, cortex lycii radicis, plantain and mulberry leaves according to the proportion of 1:1:1:1:1, and fully and uniformly stirring for later use.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 7:3:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
The products of the above embodiments: all are brown powder with pungent taste.
The application range of the products of the above embodiments is as follows: is suitable for all seawater and fresh water fish, shrimp, crab, shellfish, sea cucumber and the like, and is especially suitable for culturing aged water bodies which have more sludge, white turbid water, rust water, black smelly water and old green water and cause poor appetite, malnutrition, abnormal body color and slow growth of aquatic animals for many years, and probiotics containQuantity 40X 108cfu/g。
The usage of the products of the above embodiments: diluting with 10-20 times of water, and sprinkling uniformly.
The specific dosage of the products of the above examples is as follows:
1. the summer heat relieving and stress resisting effects are that 500 g of the product is used for 250 times per mu of water surface (water depth is 1 m) for the first time, 100 g of the product is used for 100 g of water surface (water depth is 1 m) for the later time, times of sprinkling are carried out every 5-7 days, and the product can be sprinkled twice when the water quality is deteriorated;
2. seedling raising, namely sprinkling 5 g of water per cubic meter and sprinkling times every 2-3 days.
The notes on the products of the above examples are as follows:
1. chemical medicines and antibiotic medicines with bactericidal action are forbidden to be used for three days before and five days after the administration of the summer-heat-removing bacteria;
2. the product is not affected by weather and water temperature, and can ensure good water quality, relieve summer heat, remove toxic substances, and promote growth;
3. the product is safe to use, can be used twice when the water quality is seriously aged, and can be changed after being used for 3 days.
Summer-heat-relieving bacteria experimental example 1:
water body treatment by experiment: taking the polder town under the Xinghua city of Jiangsu province, breeding two adjacent larval crabs in a pond with about 1 mu, adopting a fine breeding feeding mode, keeping the water depth about 1.2 m, enabling the breeding pond to be fine sand muddy, cleaning the bottom of the pond, leveling the slope of the pond, and enabling the slope ratio to be 1:3, arranging water inlet and outlet gates at two ends of the cultivation pool, and installing fence protecting nets so as to prevent escape and exit from the pool. The blocking and protecting net is made of nylon bolting silk, the periphery of the cultivating pool is provided with escape-proof walls, the height of the walls is 30-40 cm, the walls are made of plastic film materials, the detained crabs can be effectively prevented from escaping, the water color is light green, the crabs do not die, the eating is normal, the detained crabs are divided into an experimental group and a control group, and the observation period is 7 months and 15 days to 8 months and 15 days.
The stocking condition and the pond condition are that 500 g of seedlings are put in each mu of the two ponds, the individual sizes are uniform, namely about 10 thousands of seedlings are planted in the two ponds, and the waterweeds are mainly planted in the ponds.
Problem analysis: influence on survival rate and weight gain rate of the crabs and comparison of main water quality indexes after the crabs are splashed with heatstroke-relieving bacteria in the whole pool during high temperature.
The treatment scheme is as follows:
the experimental group is that the heatstroke-relieving bacteria No. 1 is sprinkled in the whole pool after being diluted by 10 times of pool water, 5 grams of heatstroke-relieving bacteria is sprinkled in each cubic water body, and times is sprinkled every 2 days.
Control group: does not use summer-heat relieving bacteria
The other feeding conditions and feeding amount are the same.
Follow-up results after treatment:
average weight of 100 juvenile crabs obtained by sampling each time
The pond water quality indexes of the control group and the experimental group are measured, times are measured every 5 days, 7 times are measured in succession, and the average value is obtained (see the following table):
case analysis:
through observation of the whole experimental process, the following conclusions can be drawn:
(), the growth speed of the crabs is obviously accelerated
During the high temperature period, the growth speed of the experimental group crabs is obviously accelerated compared with that of the control group crabs, and the final average weight of the experimental group crabs is increased by 15.9% compared with that of the control group crabs.
(II) the survival rate of the crabs is obviously improved
During the high temperature period, the survival rate of the crabs is improved by 4.33 percent after the experimental group is put in the summer-heat removing bacteria compared with the survival rate of the crabs in the control group.
(III) the water quality is obviously improved
After the test pool uses the summer-heat-removing bacteria, the water quality is obviously improved, and the pH value is always stable between 7.5 and 8.2; meanwhile, the dissolved oxygen in the water body can be indirectly improved, so that the water quality is fresh. The transparency of the water body is kept about 60-80 cm all the time.
As shown in the graphs of fig. 1 and fig. 2, the contents of harmful substances such as ammonia nitrogen and nitrite in the experimental group are obviously reduced after the observation period compared with the comparative group, and the contents of ammonia nitrogen and nitrite in the water body of the experimental group are relatively stable during the observation period and can be controlled within a safe range.
In conclusion, the summer-heat relieving bacteria can promote the aquatic animals to absorb and utilize functional substances such as summer-heat relieving, heat reducing, stress resisting and the like in the herbaceous plants by adjusting the structure of the advantageous micro-ecological flora in the intestinal tracts of the aquatic animals, so that the high tolerance of the aquatic animals to the water temperature of the pond in summer severe summer is enhanced, and the aims of resisting summer heat, increasing weight and improving survival rate are fulfilled. Meanwhile, the heatstroke-relieving bacteria sprayed in the whole pool can obviously improve the water quality.
Summer-heat-relieving bacteria experimental example 2:
water body treatment by experiment: taking two adjacent sea cucumber culture ponds with water surfaces of 100 mu in Nancun of Jincano island warehouse in Laizhou city of Shandong tobacco terrace, wherein the water depth is about 1.5 m, the water level in high-temperature seasons is increased to 2 m, the putting time of sea cucumber seedlings is 5 months and 10 days, the putting amount is 8000 heads/mu, the sea cucumber culture ponds are divided into an experimental group and a control group, and the processing and observation period is 4 months and is 6 months and 1 day to 9 months and 30 days. Samples were observed and recorded every half month.
The breeding condition is that the sea cucumber seedlings are about 50 g, the specification is basically , the body surface is not damaged, the vigor is strong, the sea cucumber thorns are complete, and the viscera are not discharged, and the sea cucumber seedlings are divided into an experimental group and a control group.
Problem analysis: influence on survival rate and weight gain rate of the sea cucumber after the sea cucumber is splashed with summer-heat-relieving bacteria in the whole pool during high temperature and comparison of main water quality indexes.
The treatment scheme is as follows:
the experimental group is that the heatstroke eliminating bacteria No. 1 is sprinkled in the whole pool after being diluted by 20 times of pool water, 500 g of heatstroke eliminating bacteria is sprinkled on each mu of water surface (the water depth is 1 m) for the first time, then 100 g of heatstroke eliminating bacteria is sprinkled on each mu of water surface (the water depth is 1 m) for the first time, and times are sprinkled every 5 days.
Control group: does not use summer-heat relieving bacteria
The other feeding conditions and feeding amount are the same
Tracking results after processing
The average weight of 100 sea cucumber seedlings in each sampling
The pond water quality indexes of the control group and the experimental group are measured, times are measured every 20 days, 7 times are measured in succession, and the average value is obtained (see the following table):
case analysis:
through observation of the whole experimental process, the following conclusions can be drawn:
() the growth rate of the sea cucumber is obviously increased
During the high temperature period, the growth speed of the sea cucumbers in the experimental group is obviously increased compared with that of the sea cucumbers in the control group, and the average weight of the sea cucumbers in the experimental group is finally increased by 29.5 percent compared with that of the sea cucumbers in the control group.
(II) the summer sleeping time of the sea cucumber is obviously shortened
In the high temperature period, the 'summer sleeping' time of the sea cucumber experimental group is averagely shortened by about 15 days compared with that of the comparative group. The average 'summer sleeping' time of the sea cucumbers in the control group is about 100 days, and the 'summer sleeping' time of the experimental group is about 85 days.
(III) the water quality is obviously improved
After the test pool is used with the heatstroke-relieving bacteria, the water quality is obviously improved, for example, harmful substances such as ammonia nitrogen, nitrite and the like in the pool are obviously reduced in an experimental group compared with a comparison group in the later period of an observation period as shown in graphs shown in fig. 3 and 4, and the pH value is always stabilized at about 8.2; the ammonia nitrogen and the nitrite in the water body are relatively stable and can be controlled within a safe range. Meanwhile, the dissolved oxygen in the water body can be indirectly improved, so that the water quality is fresh.
In conclusion, the summer-heat relieving bacteria can promote the aquatic animals to absorb and utilize functional substances such as summer-heat relieving, heat reducing, stress resisting and the like in herbaceous plants put in water by adjusting the structure of the advantageous micro-ecological flora of the aquaculture pond, so that the high tolerance of the aquatic animals to the water temperature of the pond in summer in hot summer is enhanced, the definite value and invasion of pathogenic bacteria of high-temperature water are inhibited, the outbreak of high-temperature pathogenic factors is limited, the purposes of resisting summer heat, increasing weight and improving the survival rate are achieved, the 'summer sleeping' time can be obviously shortened aiming at sea cucumbers, the food intake is improved, and the immunity of the aquatic animals is enhanced.
In addition, the same results as described above were obtained with the products of examples 2 to 4.
Claims (10)
1, splash-type summer-heat relieving bacteria, which is characterized in that the summer-heat relieving bacteria is prepared by evenly mixing the following components , component two and component three, crushing and filtering with a screen mesh:
wherein the component is a composite strain synthetic material;
the second component is a mixture of leguminous plants and zingiberaceae plants;
the third component is a herbaceous plant mixture;
the herbal mixture is selected from more than of mint, houttuynia cordata, cortex lycii radicis, plantain and mulberry leaves, and the mass ratio of the component , the component two and the component three is 6-10:1-3: 1.
2. The species of the splashing summer-heat-removing fungus of claim 1, wherein:
the component is prepared by mixing composite strains, aerobic bacteria and zeolite powder;
the mass part ratio of the composite strain, the aerobic bacteria and the zeolite powder is 20-35: 1: 50-80 parts;
wherein the composite strain is a fermentation product of EM strain.
3. The species of the splashing summer-heat-removing fungus of claim 2, wherein:
the preparation method of the composite strain is as follows: taking EM strain and saline water according to the ratio of 8-20: 1, pouring into a fermentation tank for fermentation, keeping the temperature at 25-30 ℃, and carrying out anaerobic fermentation culture for 4-10 days to obtain the composite strain.
4. The species of the splashing summer-heat-removing fungus of claim 3, wherein:
the saline water is a sodium chloride and/or potassium chloride solution with the mass concentration of 1-5%.
5. The species of the splashing summer-heat-removing fungus of claim 1, wherein:
the mass ratio of the leguminous plants to the zingiberaceae plants is 1: 2-5.
6. The species of the splashing summer-heat-removing fungus of claim 5, wherein:
the plant of Leguminosae is selected from radix Sophorae Flavescentis.
7. The species of the splashing summer-heat-removing fungus of claim 1, wherein:
the herbaceous plants in the herbaceous plant mixture are used in equal amount.
8. The use method of splash-type sunstroke-relieving bacteria as claimed in any one of claims 1-7 and , wherein the water is diluted by 10-20 times of the target amount and then uniformly sprinkled in the target water pool.
9. The use of species of sprinkled-type summer-heat removing bacteria, as claimed in any one of claims 1-7 and , wherein:
the additive is used in high-temperature seasons, helps the aquatic animals relieve summer heat and reduce fever, and improves the anti-stress capability;
and/or
The microbial agent is used for inhibiting the colonization and invasion of pathogenic bacteria in a high-temperature water body and limiting the outbreak of high-temperature pathogenic factors; and/or
The functions of regulating fat metabolism, resisting genetic material DNA variation and repairing cell damage;
and/or
The product has effects in resisting free radical injury, reducing lipid peroxide, inhibiting aquatic animal viscera necrosis, and protecting health;
and/or
Is used for promoting secretion of endogenous digestive enzyme, improving appetite of aquatic animals, and enhancing digestion and absorption of feed.
10. The use of kinds of sprinkling-type summer-heat removing bacteria, according to claim 9, wherein:
when the water quality improver is used for improving the anti-stress capability, 500 g/mu is used for the first time every 250-mu, and times are splashed every 5-7 days after 50-100 g/mu, and the water quality is splashed twice by 1-3 times when the water quality is deteriorated;
when used for raising seedlings, 1 to 5 grams of water is sprinkled on each cubic meter of water, and times of sprinkling are sprinkled every 2 to 3 days.
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| CN201911174610.2A CN110731282A (en) | 2019-11-26 | 2019-11-26 | sprinkling summer-heat-relieving bacteria |
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Application publication date: 20200131 |