CN105614880A - Biological agent for repairing irritable bowel mucosa damage and preparation method thereof - Google Patents
Biological agent for repairing irritable bowel mucosa damage and preparation method thereof Download PDFInfo
- Publication number
- CN105614880A CN105614880A CN201510999991.3A CN201510999991A CN105614880A CN 105614880 A CN105614880 A CN 105614880A CN 201510999991 A CN201510999991 A CN 201510999991A CN 105614880 A CN105614880 A CN 105614880A
- Authority
- CN
- China
- Prior art keywords
- preparation
- weight proportion
- water
- concentrated
- brown sugar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000006378 damage Effects 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 210000004877 mucosa Anatomy 0.000 title claims abstract description 19
- 239000003124 biologic agent Substances 0.000 title abstract 4
- 208000002551 irritable bowel syndrome Diseases 0.000 title abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 19
- 241000196324 Embryophyta Species 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 16
- 235000003145 Hippophae rhamnoides Nutrition 0.000 claims abstract description 11
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 11
- 244000046146 Pueraria lobata Species 0.000 claims abstract description 11
- 235000010575 Pueraria lobata Nutrition 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims abstract description 9
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 6
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 6
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 6
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 6
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 6
- 240000000950 Hippophae rhamnoides Species 0.000 claims abstract 3
- 239000000203 mixture Substances 0.000 claims description 34
- 238000009472 formulation Methods 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 28
- 230000001580 bacterial effect Effects 0.000 claims description 26
- 208000027418 Wounds and injury Diseases 0.000 claims description 23
- 208000014674 injury Diseases 0.000 claims description 22
- 206010022998 Irritability Diseases 0.000 claims description 17
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims description 16
- 239000004386 Erythritol Substances 0.000 claims description 16
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 claims description 16
- 229940009714 erythritol Drugs 0.000 claims description 16
- 235000019414 erythritol Nutrition 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 16
- 230000001681 protective effect Effects 0.000 claims description 16
- 239000007787 solid Substances 0.000 claims description 14
- 241000186660 Lactobacillus Species 0.000 claims description 13
- 239000002131 composite material Substances 0.000 claims description 13
- 229940039696 lactobacillus Drugs 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 12
- 235000009392 Vitis Nutrition 0.000 claims description 10
- 241000219095 Vitis Species 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 210000000582 semen Anatomy 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 9
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 8
- 239000008101 lactose Substances 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 8
- 238000007493 shaping process Methods 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 229940081969 saccharomyces cerevisiae Drugs 0.000 claims description 5
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 3
- 239000012669 liquid formulation Substances 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 230000000968 intestinal effect Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract 6
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 239000002609 medium Substances 0.000 abstract 2
- 235000009754 Vitis X bourquina Nutrition 0.000 abstract 1
- 235000012333 Vitis X labruscana Nutrition 0.000 abstract 1
- 240000006365 Vitis vinifera Species 0.000 abstract 1
- 235000014787 Vitis vinifera Nutrition 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 18
- 241000229143 Hippophae Species 0.000 description 8
- 210000004347 intestinal mucosa Anatomy 0.000 description 8
- 239000003963 antioxidant agent Substances 0.000 description 5
- 230000003078 antioxidant effect Effects 0.000 description 5
- 235000006708 antioxidants Nutrition 0.000 description 5
- 230000004888 barrier function Effects 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 241000700159 Rattus Species 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 150000003254 radicals Chemical class 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000014590 basal diet Nutrition 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000036542 oxidative stress Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 206010061172 Gastrointestinal injury Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010033645 Pancreatitis Diseases 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 206010051379 Systemic Inflammatory Response Syndrome Diseases 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000004913 chyme Anatomy 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000013003 healing agent Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000005027 intestinal barrier Anatomy 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 230000004673 intestinal mucosal barrier function Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical class CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000021238 nutrient digestion Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a biological agent for repairing irritable bowel mucosa damage and a preparation method thereof. The method comprises the steps that brewer's yeast, lactobacillus acidophilus and lactobacillus plantarum are mixed to obtain mixed bacteria; the mixed bacteria are added to a brown sugar water culture medium, airtight culture is carried out till the pH is smaller than 4.00, then culture continues to be carried out for 20-30 days, and compound bacteria are obtained; kudzu vine roots, sea-buckthorns, grape seeds, brown sugar and water are mixed to obtain a compound medium; the compound bacteria are added to the compound medium, airtight culture is carried out for 30-45 days, and a compound fermentation product is obtained; then soaking, elution, concentrating, sterilizing and forming are carried out, and the biological agent is obtained. The effective ingredients of plants are converted through the microorganism compound fermentation technology, and therefore the product has the advantages of being easier to absorb and utilize, remarkable in treatment effect and the like, especially free radicals can be scavenged, intestinal flora balance can be adjusted, repairing of the irritable bowel mucosa damage is promoted, and the biological agent for repairing the irritable bowel mucosa damage is natural.
Description
Technical field
The present invention relates to a kind of field of health care food, it is specifically related to a kind of biotechnological formulation, particularly relate to a kind of biotechnological formulation for repairing irritability enteron aisle mucosa injury and its preparation method.
Background technology
Enteron aisle, the main place being not only in human body nutrient digestion and absorption, and be target organ the most easily damaged in multiple organs dysfunction syndrome, systemic inflammatory response syndrome. The intestinal mucosa barrier of normal human is made up of mechanical barrier, membranes barriers and immunization barrier. Perfect intestinal mucosa defense system can effectively stop the objectionable impurities in enteron aisle to enter body circulation.
At present, when human body is under stress situation, its enteron aisle can produce a large amount of free radicals, thus intestinal mucosa can be caused to damage. Further, using microbiotic, hemorrhage, shock in a large number, continuing the clinical manifestation that intestinal mucosal barrier damages also all likely to occur when total parenteral nutritionTPN, wound, serious burn, acute critical pancreatitis, liver cirrhosis, chemicotherapy.
In the prior art, also having a lot about the research and technical scheme repairing intestinal mucosa damage, wherein, CN103349153A discloses a kind of thalline mixture and the production method thereof with animal intestinal repairing effect; CN100502940 discloses a kind of healing agent for intestinal mucosal injury and its preparation method. In fact; it is well known by persons skilled in the art that probiotic bacterium can change intestinal health; wherein, specifically comprise improve bacterium group ratio in enteron aisle and transform some enteral prods, anti-binding site short of money thus stop pathogenic bacterium field planting, regulate the balance between anti-inflammation and the raw scorching factor and then promote the reparation of intestinal tract injury epithelium and strengthen epithelial tight to be connected, strengthen intestinal mucosa barrier protection effect thus stop bacterium displacement etc. Above prior art and correlative study achievement are all adopt beneficial flora protected by the gastrointestinal injury of generality or repair; and for the intestinal tract injury that oxidative stress causes; especially the intestinal mucosa damage caused for the generation of a large amount of free radical, does not still well repair method at present.
Summary of the invention
Based on above-mentioned background technology, the present invention provides a kind of biotechnological formulation for repairing irritability enteron aisle mucosa injury and its preparation method, by microorganism complex ferment technology, the effective constituent of plant is transformed, the described biotechnological formulation prepared is made to have features such as more easily absorbing and utilize, result for the treatment of is remarkable, especially can scavenging free radicals, regulating intestinal canal colony balance, promoting to repair irritability enteron aisle mucosa injury, being a kind of natural biological preparation for the damage of irritability intestinal mucosa being carried out restoration of the ecosystem.
The technical scheme of the present invention comprises the preparation method of a kind of biotechnological formulation for repairing irritability enteron aisle mucosa injury, it is characterised in that, comprising:
Step 1: be mixed to get mixed bacterium according to the weight proportion of yeast saccharomyces cerevisiae 20-35 part, Lactobacterium acidophilum 25-40 part, plant lactobacillus 20-35 part, added in brown sugar water substratum, under 28-32 DEG C of condition, the airtight pH of being cultured to is less than 3.60-4.00, then continue to cultivate 20-30 days, obtain composite bacteria;
Step 2: be mixed to get complex medium according to the weight proportion of root of kudzu vine 10-25 part, sea-buckthorn 15-25 part, Semen Vitis viniferae 20-30 part, brown sugar 1-3 part, water 40-60 part;
Step 3: described composite bacteria is added in described complex medium, under 28-32 DEG C of condition, airtight cultivation 30-45 days, obtain complex ferment thing;
Step 4: soaked by described complex ferment thing, drip washing, concentrated, sterilizing, shaping, obtains described biotechnological formulation.
In one embodiment of the invention, wherein:
Described yeast saccharomyces cerevisiae (Saccharomycescerevisiae) for deposit number be the bacterial classification of ACCC20065;
Described Lactobacterium acidophilum (Lactobacillusacidophilus) for deposit number be the bacterial classification of ACCC10637;
Described plant lactobacillus (Lactobacillusplantarum) for deposit number be the bacterial classification of ACCC11118.
In a preferred embodiment of the invention, described preparation method also comprises the cultivation of bacterial classification, is specially:
Being accessed in potato liquid nutrient medium by yeast saccharomyces cerevisiae bacterial classification and carry out shaking culture, wherein, culture temperature is 28-32 DEG C, the revolution of shaking culture is 180-220rpm, duration of oscillation is 48-72 hour, takes out, put into 2-4 DEG C of Refrigerator store stand-by after substratum muddiness;
Lactobacillus acidophilus species, plant lactobacillus bacterial classification being accessed respectively and carry out quiescent culture in MRS liquid nutrient medium, wherein, culture temperature is 35-39 DEG C, and incubation time is 48-72 hour, takes out, put into 2-4 DEG C of Refrigerator store stand-by after substratum muddiness.
In an embodiment, about step 1, it may be preferred that described mixed bacterium is added in brown sugar water substratum according to the weight proportion of 1:80-120, wherein, the component of described brown sugar water substratum and weight proportion thereof are brown sugar 5-10 part, water 90-98 part.
In another embodiment, about step 2, it may be preferred that the described root of kudzu vine, sea-buckthorn, Semen Vitis viniferae are pulverized by pulverizer, obtain 30-50 order particle.
In an embodiment, it may be preferred that about step 3, described composite bacteria is added in described complex medium according to the weight proportion of 1:15-20.
In yet another embodiment, it may be preferred that about step 4, wherein:
By adding in described complex ferment thing, to add water to solid liquid weight proportion be 1:1, soaks 1-2 days;
Then drip washing, and leacheate is concentrated to 20-30% under 40-50 DEG C of condition, obtain concentrated solution;
Further, sterilizing 10-30 minute under 60-70 DEG C of condition, obtains concentrated sterilized solution;
Described shaping be the further course of processing, wherein, described biotechnological formulation can be solid formulation, it is also possible to be liquid formulation, such as oral liquid;
Wherein, further ground procedure of processing about solid formulation is: according to concentrated sterilized solution 15-30 part, erythritol 15-30 part, lactose 30-60 part, the weight proportion of protective material 3-5 part takes, first described erythritol and lactose are added in spraying dry one-step-granulating method successively, then start to spray into described concentrated sterilized solution, flow is 10-20ml/min, finally spray into the protective material being dissolved in the water, wherein, the inlet temperature of described nodulizer is 60-80 DEG C, air outlet temperature is 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in device to be sprayed, obtain described solid formulation,
Wherein, the further ground procedure of processing of described oral liquid is: take according to the weight proportion of concentrated sterilized solution 45-50 part, erythritol 45-50 part, protective material 5-10 part; first dissolve described erythritol and protective material add appropriate hot water stirs; to be cooled to after 25-35 DEG C; add described concentrated sterilized solution; stir to clarify, obtain described oral liquid.
The technical scheme of the present invention also comprises the described biotechnological formulation that a kind of preparation method described above prepares, for repairing irritability enteron aisle mucosa injury.
Present inventor finds, the Radical Metabolism of antioxidant controllable oxidative stress animal, and contained effective constituent can play its effect effectively in the described biotechnological formulation obtained by technique scheme, especially for reparation irritability enteron aisle mucosa injury, especially applicable.
The present invention adopts country to defend the bacterial classification in " can be used for the bacterial classification list of food " of planning commission's bulletin, adopts conventional plant and fruit to be raw material, carries out complex ferment and prepares a kind of natural biological preparation. The present invention combines the first kind in existing healthcare products and the effective constituent of the 2nd class, product is rich in the activeconstituents of a large amount of resistance of oxidation, such as: flavonoid (4.43%), soaping agents (82.4mg/100g), amino acid (4.21%), gsh (886mg/100g), vitamins C (322mg/100g), vitamin-E (908 �� g/100g), SOD (194000U/100g), lactic acid salt (5.04%) and various trace elements (iron: 503mg/kg; Selenium: 0.18mg/kg etc.) etc.
The technical scheme of the present invention has the function promoting that irritability enteron aisle mucosa injury is repaired, by microorganism complex ferment technology, the effective constituent of plant is transformed, make it have and more easily absorb, the features such as effect is remarkable are a kind of natural biological preparations that the damage of irritability intestinal mucosa can carry out restoration of the ecosystem.
Embodiment
The present invention provides the preparation method of a kind of biotechnological formulation for repairing irritability enteron aisle mucosa injury, it is characterised in that, comprising:
Step 1: be mixed to get mixed bacterium according to the weight proportion of yeast saccharomyces cerevisiae 20-35 part, Lactobacterium acidophilum 25-40 part, plant lactobacillus 20-35 part, added in brown sugar water substratum, under 28-32 DEG C of condition, the airtight pH of being cultured to is less than 3.60-4.00, then continue to cultivate 20-30 days, obtain composite bacteria;
Step 2: be mixed to get complex medium according to the weight proportion of root of kudzu vine 10-25 part, sea-buckthorn 15-25 part, Semen Vitis viniferae 20-30 part, brown sugar 1-3 part, water 40-60 part;
Step 3: described composite bacteria is added in described complex medium, under 28-32 DEG C of condition, airtight cultivation 30-45 days, obtain complex ferment thing;
Step 4: soaked by described complex ferment thing, drip washing, concentrated, sterilizing, shaping, obtains described biotechnological formulation.
In one embodiment of the invention, wherein:
Described yeast saccharomyces cerevisiae (Saccharomycescerevisiae) for deposit number be the bacterial classification of ACCC20065;
Described Lactobacterium acidophilum (Lactobacillusacidophilus) for deposit number be the bacterial classification of ACCC10637;
Described plant lactobacillus (Lactobacillusplantarum) for deposit number be the bacterial classification of ACCC11118.
In a preferred embodiment of the invention, described preparation method also comprises the cultivation of bacterial classification, is specially:
Being accessed in potato liquid nutrient medium by yeast saccharomyces cerevisiae bacterial classification and carry out shaking culture, wherein, culture temperature is 28-32 DEG C, the revolution of shaking culture is 180-220rpm, duration of oscillation is 48-72 hour, takes out, put into 2-4 DEG C of Refrigerator store stand-by after substratum muddiness;
Lactobacillus acidophilus species, plant lactobacillus bacterial classification being accessed respectively and carry out quiescent culture in MRS liquid nutrient medium, wherein, culture temperature is 35-39 DEG C, and incubation time is 48-72 hour, takes out, put into 2-4 DEG C of Refrigerator store stand-by after substratum muddiness.
In an embodiment, about step 1, it may be preferred that described mixed bacterium is added in brown sugar water substratum according to the weight proportion of 1:80-120, wherein, the component of described brown sugar water substratum and weight proportion thereof are brown sugar 5-10 part, water 90-98 part.
In another embodiment, about step 2, it may be preferred that the described root of kudzu vine, sea-buckthorn, Semen Vitis viniferae are pulverized by pulverizer, obtain 30-50 order particle.
In an embodiment, it may be preferred that about step 3, described composite bacteria is added in described complex medium according to the weight proportion of 1:15-20.
In yet another embodiment, it may be preferred that about step 4, wherein:
By adding in described complex ferment thing, to add water to solid liquid weight proportion be 1:1, soaks 1-2 days;
Then drip washing, and leacheate is concentrated to 20-30% under 40-50 DEG C of condition, obtain concentrated solution;
Further, sterilizing 10-30 minute under 60-70 DEG C of condition, obtains concentrated sterilized solution;
Described shaping be the further course of processing, wherein, described biotechnological formulation can be solid formulation, it is also possible to be liquid formulation, such as oral liquid;
Wherein, further ground procedure of processing about solid formulation is: according to concentrated sterilized solution 15-30 part, erythritol 15-30 part, lactose 30-60 part, the weight proportion of protective material 3-5 part takes, first described erythritol and lactose are added in spraying dry one-step-granulating method successively, then start to spray into described concentrated sterilized solution, flow is 10-20ml/min, finally spray into the protective material being dissolved in the water, wherein, the inlet temperature of described nodulizer is 60-80 DEG C, air outlet temperature is 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in device to be sprayed, obtain described solid formulation,
Wherein, the further ground procedure of processing of described oral liquid is: take according to the weight proportion of concentrated sterilized solution 45-50 part, erythritol 45-50 part, protective material 5-10 part; first dissolve described erythritol and protective material add appropriate hot water stirs; to be cooled to after 25-35 DEG C; add described concentrated sterilized solution; stir to clarify, obtain described oral liquid.
The present invention provides the described biotechnological formulation that a kind of preparation method described above prepares, for repairing irritability enteron aisle mucosa injury.
Wherein, it should be noted that when there is no specified otherwise, the content that the present invention relates to or proportioning refer to weight content or weight proportion.
Technique scheme according to the present invention, now does explain further and illustrate to the content of the present invention by following examples.
Embodiment 1
For repairing a preparation method for the biotechnological formulation of irritability enteron aisle mucosa injury, comprising:
The first step: the cultivation of bacterial classification: the yeast saccharomyces cerevisiae bacterial classification (Saccharomycescerevisiae that will buy from Chinese agriculture Microbiological Culture Collection administrative center, ACCC20065) access in the Erlenmeyer flask of potato liquid nutrient medium, at 28 DEG C, 180-220rpm shaking culture 48 hours, takes out after substratum muddiness; By Lactobacillus acidophilus species (Lactobacillusacidophilus, ACCC10637), plant lactobacillus bacterial classification (Lactobacillusplantarum, ACCC11118), access in the Erlenmeyer flask of MRS liquid nutrient medium respectively, cultivate 48 hours under 37 DEG C of conditions, until taking out after substratum muddiness, then put into 4 DEG C of Refrigerator stores stand-by;
2nd step: be mixed to get mixed bacterium according to the weight proportion of yeast saccharomyces cerevisiae 35 parts, Lactobacterium acidophilum 30 parts, plant lactobacillus 35 parts, added in brown sugar water substratum according to the part by weight of 1:100, wherein, 5 parts, brown sugar, pure water 95 parts, after fermentor tank stirring evenly, under 30 DEG C of conditions, the airtight pH of being cultured to is less than 3.60, then continue to cultivate 25 days, obtain composite bacteria;
3rd step: filter out the individual uniform root of kudzu vine, sea-buckthorn, Semen Vitis viniferae, and pulverize into 40 orders by pulverizer respectively, be mixed to get complex medium according to the weight proportion of the root of kudzu vine 25 parts, sea-buckthorn 20 parts, Semen Vitis viniferae 20 parts, 3 parts, brown sugar, 45 parts, water;
4th step: add in described complex medium by the part by weight of 1:20 by described composite bacteria, stirs after evenly airtight cultivation 15 days under 30 DEG C of conditions at fermentor tank, obtains complex ferment thing;
5th step: add water in fermentor tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing; Leacheate carries out concentrating to 20% at 50 DEG C; Concentrated solution at 60 DEG C sterilization after 20 minutes 4 DEG C of storages stand-by;
6th step: the preparation of solid dosage: take according to the weight proportion of concentrated sterilized solution 20 parts, erythritol 20 parts, lactose 55 parts, protective material 5 parts; first described erythritol and lactose are added in spraying dry one-step-granulating method successively; then start to spray into described concentrated sterilized solution; flow is 10ml/min; finally spray into the protective material being dissolved in suitable quantity of water; wherein; the inlet temperature of described nodulizer is 60 DEG C; air outlet temperature is 45 DEG C; discharging when temperature drops to 30 DEG C in device to be sprayed, obtains described solid formulation.
Embodiment 2
For repairing a preparation method for the biotechnological formulation of irritability enteron aisle mucosa injury, comprising:
The first step: the cultivation of bacterial classification: the yeast saccharomyces cerevisiae bacterial classification (Saccharomycescerevisiae that will buy from Chinese agriculture Microbiological Culture Collection administrative center, ACCC20065) access in the Erlenmeyer flask of potato liquid nutrient medium, at 28 DEG C, 180-220rpm shaking culture 48 hours, takes out after substratum muddiness; By Lactobacillus acidophilus species (Lactobacillusacidophilus, ACCC10637), plant lactobacillus bacterial classification (Lactobacillusplantarum, ACCC11118), access in the Erlenmeyer flask of MRS liquid nutrient medium respectively, cultivate 48 hours under 37 DEG C of conditions, until taking out after substratum muddiness, then put into 4 DEG C of Refrigerator stores stand-by;
2nd step: be mixed to get mixed bacterium according to the weight proportion of yeast saccharomyces cerevisiae 20 parts, Lactobacterium acidophilum 35 parts, plant lactobacillus 30 parts, added in brown sugar water substratum according to the part by weight of 1:100, wherein, 3 parts, brown sugar, pure water 97 parts, after fermentor tank stirring evenly, under 32 DEG C of conditions, the airtight pH of being cultured to is less than 3.60, then continue to cultivate 22 days, obtain composite bacteria;
3rd step: filter out the individual uniform root of kudzu vine, sea-buckthorn, Semen Vitis viniferae, and pulverize into 40 orders by pulverizer respectively, be mixed to get complex medium according to the weight proportion of the root of kudzu vine 20 parts, sea-buckthorn 15 parts, Semen Vitis viniferae 25 parts, 2 parts, brown sugar, 40 parts, water;
4th step: add in described complex medium by the part by weight of 1:15 by described composite bacteria, stirs after evenly airtight cultivation 12 days under 32 DEG C of conditions at fermentor tank, obtains complex ferment thing;
5th step: add water in fermentor tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing; Leacheate carries out concentrating to 20% at 45 DEG C; Concentrated solution at 65 DEG C sterilization after 10 minutes 4 DEG C of storages stand-by;
6th step: the preparation of oral liquid: take according to the weight proportion of concentrated sterilized solution 45 parts, erythritol 45 parts, protective material 10 parts; first described erythritol and protective material will add appropriate hot distilled water stirring and dissolving; to be cooled to after 35 DEG C; add described concentrated sterilized solution; stir to clarify, obtain described oral liquid.
Detection embodiment
Animal divides into groups: selecting healthy cleaning grade SD rat age on the 42nd, no-special pathogen (SPF) level, body weight (200 �� 20) g, is divided into 3 groups at random, often organizes 12 (male and female account for 1/2). The determination of dosage: control group and induction group are fed basal diet, injury repairing group feed basal diet and in drinking-water by every every day 0.8mL add microbial origin antioxidant. The the 5th, 9,13,17 day every kg body weight abdominal injection 0.8mgLPS that induction group and injury repairing group are being tested, control group injection equivalent physiological saline. Trial period, is 22d altogether.
Index detection method:; Rat adopts eyeball put to death method put to death, open abdominal cavity in intestinal tissue, cut the intestines section of nearly 1cm in jejunum stage casing, put into rapidly stationary liquid, shake even, tissue slice to be done. By fixing sample through washing, transparent, leaching wax, embedding etc. process after, at room temperature it is cut into the section of 8um, hematoxylin eosin stain, 5 typical visuals field (fine hair complete, move towards straight) are selected in the section of each tissue site, measures height of naps, the Crypt depth at the widest the longest fine hair place in each visual field with ocular micrometer. Putting the small intestine under room temperature longitudinally to cut off at the forward position intestinal tube of thawing completely, take out chyme, scrape mucous membrane and doubly dilute rear homogenate with the 0.4mol/L Klorvess Liquid of 4 DEG C by 3-10 after mixing and centrifugal, supernatant liquor is as preservation at-20 DEG C.
Table 1 is that microbial origin antioxidant is on the impact of LPS injury rats intestinal tissue form
| Project | Control group | Induction group | Reparation group |
| Height of naps (��m) | 262.84��8.43 | 259.22��4.25 | 273.08��4.52 |
| Crypt depth (��m) | 275.37��12.12 | 284.96��15.37 | 253.62��6.13 |
| Height of naps/Crypt depth | 0.96��0.05 | 0.90��0.05 | 1.06��0.04 |
Can obtain by table 1: intestinal tissue form aspect, compared with control group, induction group rat enteron aisle height of naps is lower slightly, and Crypt depth is slightly dark, and height of naps and Crypt depth ratio are lower slightly, but difference is not significantly (P>0.05), and injury repairing group is compared with control group, height of naps is higher, and Crypt depth is more shallow, height of naps and Crypt depth ratio are relatively big, significant difference (P<0.05). Therefore, add microbial origin antioxidant to maintenance enteron aisle normal configuration aspect, repair irritability enteron aisle mucosa injury and there is good effect.
Further, adopting same above-mentioned requirement of experiment, the microbial origin antioxidant in reparation group is replaced the biotechnological formulation for preparing in embodiment 1 or 2, finally detection finds that its These parameters is close with reparation group, is even better than reparation group. Being used for by the biotechnological formulation that embodiment 1 or 2 obtains in the reparation of mouse irritability enteron aisle mucosa injury further, effect is remarkable.
Specific embodiments of the invention being described in detail above, but it is just as example, the present invention is not restricted to specific embodiment described above. To those skilled in the art, any equivalent modifications the present invention carried out and replacement are also all among the category of the present invention. Therefore, the impartial conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (10)
1. one kind for repairing the preparation method of the biotechnological formulation of irritability enteron aisle mucosa injury, it is characterised in that, comprising:
Step 1: be mixed to get mixed bacterium according to the weight proportion of yeast saccharomyces cerevisiae 20-35 part, Lactobacterium acidophilum 25-40 part, plant lactobacillus 20-35 part, added in brown sugar water substratum, under 28-32 DEG C of condition, the airtight pH of being cultured to is less than 4.00, then continue to cultivate 20-30 days, obtain composite bacteria;
Step 2: be mixed to get complex medium according to the weight proportion of root of kudzu vine 10-25 part, sea-buckthorn 15-25 part, Semen Vitis viniferae 20-30 part, brown sugar 1-3 part, water 40-60 part;
Step 3: described composite bacteria is added in described complex medium, under 28-32 DEG C of condition, airtight cultivation 30-45 days, obtain complex ferment thing;
Step 4: soaked by described complex ferment thing, drip washing, concentrated, sterilizing, shaping, obtains described biotechnological formulation.
2. preparation method according to claim 1, it is characterised in that, described yeast saccharomyces cerevisiae (Saccharomycescerevisiae) for deposit number be the bacterial classification of ACCC20065; Described Lactobacterium acidophilum (Lactobacillusacidophilus) for deposit number be the bacterial classification of ACCC10637; Described plant lactobacillus (Lactobacillusplantarum) for deposit number be the bacterial classification of ACCC11118.
3. preparation method according to claim 1, it is characterised in that, also comprise the cultivation of bacterial classification, it is specially:
Being accessed in potato liquid nutrient medium by yeast saccharomyces cerevisiae bacterial classification and carry out shaking culture, wherein, culture temperature is 28-32 DEG C, the revolution of shaking culture is 180-220rpm, duration of oscillation is 48-72 hour, takes out, put into 2-4 DEG C of Refrigerator store stand-by after substratum muddiness;
Lactobacillus acidophilus species, plant lactobacillus bacterial classification being accessed respectively and carry out quiescent culture in MRS liquid nutrient medium, wherein, culture temperature is 35-39 DEG C, and incubation time is 48-72 hour, takes out, put into 2-4 DEG C of Refrigerator store stand-by after substratum muddiness.
4. preparation method according to claim 1, it is characterized in that, about step 1, described mixed bacterium is added in brown sugar water substratum according to the weight proportion of 1:80-120, wherein, the component of described brown sugar water substratum and weight proportion thereof are brown sugar 5-10 part, water 90-98 part.
5. preparation method according to claim 1, it is characterised in that, about step 2, the described root of kudzu vine, sea-buckthorn, Semen Vitis viniferae are pulverized by pulverizer, obtain 30-50 order particle.
6. preparation method according to claim 1, it is characterised in that, about step 3, described composite bacteria is added in described complex medium according to the weight proportion of 1:15-20.
7. preparation method according to claim 1, it is characterised in that, about step 4, wherein:
By adding in described complex ferment thing, to add water to solid liquid weight proportion be 1:1, soaks 1-2 days;
Then drip washing, and leacheate is concentrated to 20-30% under 40-50 DEG C of condition, obtain concentrated solution;
Further, sterilizing 10-30 minute under 60-70 DEG C of condition, obtains concentrated sterilized solution.
8. preparation method according to claim 1, it is characterized in that, described shaping be the further course of processing, wherein, described biotechnological formulation is solid formulation, then further ground procedure of processing about solid formulation is: according to concentrated sterilized solution 15-30 part, erythritol 15-30 part, lactose 30-60 part, the weight proportion of protective material 3-5 part takes, first described erythritol and lactose are added in spraying dry one-step-granulating method successively, then start to spray into described concentrated sterilized solution, flow is 10-20ml/min, finally spray into the protective material being dissolved in the water, wherein, the inlet temperature of described nodulizer is 60-80 DEG C, air outlet temperature is 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in device to be sprayed, obtain described solid formulation.
9. preparation method according to claim 1; it is characterized in that; described biotechnological formulation is liquid formulation; such as oral liquid, then the further ground procedure of processing of described oral liquid is: take according to the weight proportion of concentrated sterilized solution 45-50 part, erythritol 45-50 part, protective material 5-10 part, first dissolves adding appropriate hot water stirs in described erythritol and protective material; to be cooled to after 25-35 DEG C; add described concentrated sterilized solution, stir to clarify, obtain described oral liquid.
10. the biotechnological formulation prepared such as preparation method as described in any one in claim 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510999991.3A CN105614880A (en) | 2015-12-28 | 2015-12-28 | Biological agent for repairing irritable bowel mucosa damage and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510999991.3A CN105614880A (en) | 2015-12-28 | 2015-12-28 | Biological agent for repairing irritable bowel mucosa damage and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105614880A true CN105614880A (en) | 2016-06-01 |
Family
ID=56030577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510999991.3A Pending CN105614880A (en) | 2015-12-28 | 2015-12-28 | Biological agent for repairing irritable bowel mucosa damage and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105614880A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108112776A (en) * | 2017-09-15 | 2018-06-05 | 普乐格（厦门）生物科技有限公司 | It is a kind of for microorganism formulation of animal and bird intestines injury repair and preparation method thereof |
| CN110314205A (en) * | 2019-05-30 | 2019-10-11 | 上海普乐拜格健康科技有限公司 | A kind of cultured fishes hepatic injury reparation natural biological preparation and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6291517B1 (en) * | 1999-11-19 | 2001-09-18 | Dry Creek Nutrition, Inc | Method for preventing or reducing stress-induced gastric injury using grape seed proanthocyanidin extract |
| CN103285106A (en) * | 2012-02-27 | 2013-09-11 | 上海中医药大学附属曙光医院 | Traditional Chinese medicinal composition for treating intestinal mucosa damage and preparation method and application thereof |
| CN103417752A (en) * | 2013-07-24 | 2013-12-04 | 上海普缇康生物技术有限公司 | Liver protection natural product and preparation method thereof |
| CN104623122A (en) * | 2015-01-12 | 2015-05-20 | 江瀚生物科技（上海）有限公司 | Biological preparation with anti-fatigue function and preparation method of biological preparation |
-
2015
- 2015-12-28 CN CN201510999991.3A patent/CN105614880A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6291517B1 (en) * | 1999-11-19 | 2001-09-18 | Dry Creek Nutrition, Inc | Method for preventing or reducing stress-induced gastric injury using grape seed proanthocyanidin extract |
| CN103285106A (en) * | 2012-02-27 | 2013-09-11 | 上海中医药大学附属曙光医院 | Traditional Chinese medicinal composition for treating intestinal mucosa damage and preparation method and application thereof |
| CN103417752A (en) * | 2013-07-24 | 2013-12-04 | 上海普缇康生物技术有限公司 | Liver protection natural product and preparation method thereof |
| CN104623122A (en) * | 2015-01-12 | 2015-05-20 | 江瀚生物科技（上海）有限公司 | Biological preparation with anti-fatigue function and preparation method of biological preparation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108112776A (en) * | 2017-09-15 | 2018-06-05 | 普乐格（厦门）生物科技有限公司 | It is a kind of for microorganism formulation of animal and bird intestines injury repair and preparation method thereof |
| CN110314205A (en) * | 2019-05-30 | 2019-10-11 | 上海普乐拜格健康科技有限公司 | A kind of cultured fishes hepatic injury reparation natural biological preparation and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102366023B (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN103609865B (en) | Pollution-free livestock and poultry feed additive using ginkgo leaves as base stock | |
| WO2007138781A1 (en) | Antiviral agent and antibacterial agent | |
| CN103355545A (en) | Fermentation type botanical weaned piglet feed additive | |
| CN103493764A (en) | Antivirus cultivation method for crabs | |
| CN106359189A (en) | Culture method of sturgeons | |
| KR101311192B1 (en) | Production method of the Lactobacilus spp. using the medium, and method of cure of herny bee's diseases | |
| CN107319196A (en) | A kind of microorganism formulation for improving fresh-water fishes function of intestinal canal and application thereof | |
| CN103626533B (en) | Bio-organic fertilizer and preparation method thereof | |
| CN105166487A (en) | Composite Chinese herbal medicine feed additive for pigs and preparation method of composite Chinese herbal medicine feed additive | |
| CN104261980A (en) | Bio-organic fertilizer for planting lotus root | |
| CN103462860B (en) | Compound preservative for cosmetics | |
| CN112741115B (en) | Composite plant extract for inhibiting Edwardsiella tarda in water environment and preparation method and application thereof | |
| CN105614880A (en) | Biological agent for repairing irritable bowel mucosa damage and preparation method thereof | |
| CN107095061B (en) | Preparation method and application of fermented feed for improving intestinal barrier function of fattening pigs | |
| CN105461456A (en) | Pennisetum hydridum ecological organic fertilizer prepared from sheep manure and preparation method thereof | |
| KR20150095323A (en) | The manufacturing method of a natural blood glucose control drink | |
| CN105617367B (en) | Promote livestock and poultry respiratory health ferment and preparation method thereof | |
| CN102669486A (en) | Special animal biogen for shrimps and preparation method thereof | |
| CN107810784A (en) | A kind of implantation methods rich in medicinal herb componentses asparagus | |
| CN109020685A (en) | A kind of microbial organic fertilizer and preparation method thereof enhancing Cotton Resistance | |
| CN105165910A (en) | Composition used for sterilizing weaving workshops | |
| CN110314186B (en) | Traditional Chinese medicine composition for preventing and treating enteritis of aquatic animals and preparation method thereof | |
| KR101750288B1 (en) | Composition for Controlling Citrus Mite and Method for Controlling Citrus Mite Using the Same | |
| CN100497209C (en) | Preparation of probiotics bacterium and method for rehabilitation of aquaculture environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160601 |