CN110669729A - 一种制备间充质干细胞外泌体的方法 - Google Patents
一种制备间充质干细胞外泌体的方法 Download PDFInfo
- Publication number
- CN110669729A CN110669729A CN201911093892.3A CN201911093892A CN110669729A CN 110669729 A CN110669729 A CN 110669729A CN 201911093892 A CN201911093892 A CN 201911093892A CN 110669729 A CN110669729 A CN 110669729A
- Authority
- CN
- China
- Prior art keywords
- mesenchymal stem
- gelatin
- culture
- stem cells
- exosome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001808 exosome Anatomy 0.000 title claims abstract description 79
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 29
- 108010010803 Gelatin Proteins 0.000 claims abstract description 42
- 239000008273 gelatin Substances 0.000 claims abstract description 42
- 229920000159 gelatin Polymers 0.000 claims abstract description 42
- 235000019322 gelatine Nutrition 0.000 claims abstract description 42
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 42
- 210000004027 cell Anatomy 0.000 claims abstract description 34
- SDZRWUKZFQQKKV-JHADDHBZSA-N cytochalasin D Chemical compound C([C@H]1[C@@H]2[C@@H](C([C@@H](O)[C@H]\3[C@]2([C@@H](/C=C/[C@@](C)(O)C(=O)[C@@H](C)C/C=C/3)OC(C)=O)C(=O)N1)=C)C)C1=CC=CC=C1 SDZRWUKZFQQKKV-JHADDHBZSA-N 0.000 claims abstract description 28
- 238000003756 stirring Methods 0.000 claims abstract description 20
- AZKVWQKMDGGDSV-BCMRRPTOSA-N Genipin Chemical compound COC(=O)C1=CO[C@@H](O)[C@@H]2C(CO)=CC[C@H]12 AZKVWQKMDGGDSV-BCMRRPTOSA-N 0.000 claims abstract description 15
- AZKVWQKMDGGDSV-UHFFFAOYSA-N genipin Natural products COC(=O)C1=COC(O)C2C(CO)=CCC12 AZKVWQKMDGGDSV-UHFFFAOYSA-N 0.000 claims abstract description 15
- 102000008070 Interferon-gamma Human genes 0.000 claims abstract description 14
- 108010074328 Interferon-gamma Proteins 0.000 claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 14
- 229960003130 interferon gamma Drugs 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims abstract description 14
- 239000004005 microsphere Substances 0.000 claims abstract description 7
- 239000003636 conditioned culture medium Substances 0.000 claims abstract description 6
- 230000010261 cell growth Effects 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 5
- 210000003954 umbilical cord Anatomy 0.000 claims description 5
- 239000012043 crude product Substances 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 238000004132 cross linking Methods 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 239000007640 basal medium Substances 0.000 claims description 2
- 210000001185 bone marrow Anatomy 0.000 claims description 2
- 238000004945 emulsification Methods 0.000 claims description 2
- 230000003169 placental effect Effects 0.000 claims 1
- 230000028327 secretion Effects 0.000 abstract description 4
- 230000004071 biological effect Effects 0.000 abstract description 2
- 210000000130 stem cell Anatomy 0.000 description 16
- 102000004169 proteins and genes Human genes 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 12
- 238000011160 research Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000017423 tissue regeneration Effects 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 239000000232 Lipid Bilayer Substances 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000008827 biological function Effects 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 102100025222 CD63 antigen Human genes 0.000 description 2
- 102100037904 CD9 antigen Human genes 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 101000934368 Homo sapiens CD63 antigen Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000009815 adipogenic differentiation Effects 0.000 description 2
- -1 amino polysaccharide Chemical class 0.000 description 2
- 230000033115 angiogenesis Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000009818 osteogenic differentiation Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000003076 paracrine Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 238000005475 siliconizing Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 208000037816 tissue injury Diseases 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010016654 Fibrosis Diseases 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- COQLPRJCUIATTQ-UHFFFAOYSA-N Uranyl acetate Chemical compound O.O.O=[U]=O.CC(O)=O.CC(O)=O COQLPRJCUIATTQ-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000002293 adipogenic effect Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008568 cell cell communication Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000001516 cell proliferation assay Methods 0.000 description 1
- 239000002458 cell surface marker Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000035992 intercellular communication Effects 0.000 description 1
- 208000023589 ischemic disease Diseases 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000000269 nucleophilic effect Effects 0.000 description 1
- 230000002188 osteogenic effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000011476 stem cell transplantation Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 231100000747 viability assay Toxicity 0.000 description 1
- 238000003026 viability measurement method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/24—Interferons [IFN]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911093892.3A CN110669729B (zh) | 2019-11-11 | 2019-11-11 | 一种制备间充质干细胞外泌体的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911093892.3A CN110669729B (zh) | 2019-11-11 | 2019-11-11 | 一种制备间充质干细胞外泌体的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110669729A true CN110669729A (zh) | 2020-01-10 |
CN110669729B CN110669729B (zh) | 2021-09-07 |
Family
ID=69086771
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201911093892.3A Active CN110669729B (zh) | 2019-11-11 | 2019-11-11 | 一种制备间充质干细胞外泌体的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110669729B (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111944747A (zh) * | 2020-08-14 | 2020-11-17 | 福建医科大学附属协和医院 | 一种用于治疗心肌梗死的人脂肪间充质干细胞外泌体及其用途 |
WO2021211569A1 (en) * | 2020-04-13 | 2021-10-21 | Massachusetts Institute Of Technology | Dissolvable gelatin-based microcarriers generated through droplet microfluidics for expansion and culture of mesenchymal stromal cell |
CN113913377A (zh) * | 2021-12-09 | 2022-01-11 | 兰州大学 | 一种可提高人间充质干细胞成骨分化效率的培养基及培养方法 |
CN114317266A (zh) * | 2021-12-31 | 2022-04-12 | 广东美赛尔细胞生物科技有限公司 | 一种间充质干细胞外泌体的制备方法及装置 |
CN114480273A (zh) * | 2022-03-07 | 2022-05-13 | 杭州荣泽生物科技集团有限公司 | 用于获得间充质干细胞及其外泌体的培养基及其制备方法 |
CN115044543A (zh) * | 2022-08-17 | 2022-09-13 | 山东卓东生物科技有限公司 | 一种提高衰老人体来源肌肉干细胞活性的方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102936578A (zh) * | 2012-11-12 | 2013-02-20 | 山东省齐鲁干细胞工程有限公司 | 一种增强间充质干细胞免疫抑制功能的方法 |
CN107475187A (zh) * | 2017-09-05 | 2017-12-15 | 山东艾克韦生物技术有限公司 | 培养液及大量获得脐带间充质干细胞外泌体的生产工艺 |
CN108094404A (zh) * | 2017-12-20 | 2018-06-01 | 北京臻惠康生物科技有限公司 | 一种改进的间充质干细胞保护液及其用途 |
CN109234231A (zh) * | 2018-11-29 | 2019-01-18 | 广州赛莱拉干细胞科技股份有限公司 | 一种规模化扩增间充质干细胞的方法 |
-
2019
- 2019-11-11 CN CN201911093892.3A patent/CN110669729B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102936578A (zh) * | 2012-11-12 | 2013-02-20 | 山东省齐鲁干细胞工程有限公司 | 一种增强间充质干细胞免疫抑制功能的方法 |
CN107475187A (zh) * | 2017-09-05 | 2017-12-15 | 山东艾克韦生物技术有限公司 | 培养液及大量获得脐带间充质干细胞外泌体的生产工艺 |
CN108094404A (zh) * | 2017-12-20 | 2018-06-01 | 北京臻惠康生物科技有限公司 | 一种改进的间充质干细胞保护液及其用途 |
CN109234231A (zh) * | 2018-11-29 | 2019-01-18 | 广州赛莱拉干细胞科技股份有限公司 | 一种规模化扩增间充质干细胞的方法 |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021211569A1 (en) * | 2020-04-13 | 2021-10-21 | Massachusetts Institute Of Technology | Dissolvable gelatin-based microcarriers generated through droplet microfluidics for expansion and culture of mesenchymal stromal cell |
CN111944747A (zh) * | 2020-08-14 | 2020-11-17 | 福建医科大学附属协和医院 | 一种用于治疗心肌梗死的人脂肪间充质干细胞外泌体及其用途 |
CN113913377A (zh) * | 2021-12-09 | 2022-01-11 | 兰州大学 | 一种可提高人间充质干细胞成骨分化效率的培养基及培养方法 |
CN113913377B (zh) * | 2021-12-09 | 2024-04-19 | 兰州大学 | 一种可提高人间充质干细胞成骨分化效率的培养基及培养方法 |
CN114317266A (zh) * | 2021-12-31 | 2022-04-12 | 广东美赛尔细胞生物科技有限公司 | 一种间充质干细胞外泌体的制备方法及装置 |
CN114317266B (zh) * | 2021-12-31 | 2023-11-07 | 广东美赛尔细胞生物科技有限公司 | 一种间充质干细胞外泌体的制备方法及装置 |
CN114480273A (zh) * | 2022-03-07 | 2022-05-13 | 杭州荣泽生物科技集团有限公司 | 用于获得间充质干细胞及其外泌体的培养基及其制备方法 |
CN115044543A (zh) * | 2022-08-17 | 2022-09-13 | 山东卓东生物科技有限公司 | 一种提高衰老人体来源肌肉干细胞活性的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN110669729B (zh) | 2021-09-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110669729B (zh) | 一种制备间充质干细胞外泌体的方法 | |
US8524492B2 (en) | Culture method for amplifying large numbers of hair follicle stem cells in vitro | |
US11339372B2 (en) | Serum-free medium inducing differentiation of umbilical cord mesenchymal stem cell into insulin-secretion-like cell and preparation method and use thereof | |
CN110475856B (zh) | 使用纳米纤维的细胞培养 | |
CN112522191A (zh) | 一种间充质干细胞的培养方法 | |
CN113423816A (zh) | 一种用于干细胞大规模制备的三维培养方法 | |
CN107446885B (zh) | 一种间充质干细胞体外成骨诱导分化的支架材料及其应用 | |
CN111621475A (zh) | 脐带间充质干细胞膜片及其制备方法 | |
CN111334469A (zh) | Pbmc体外3d甲基纤维素琼脂糖水凝胶培养基及其制备方法 | |
WO2022068029A1 (zh) | 一种3d生物组织专用培养装置和块状培育肉的制备方法 | |
CN116077448B (zh) | 人间充质干细胞注射液及其应用 | |
CN115531297B (zh) | 负载富血小板血浆和脐带间充质干细胞球的可注射水凝胶系统及其制备方法和应用 | |
CN108034634B (zh) | 一种从经血中分离宫内膜间充质干细胞的方法 | |
JP2018078862A (ja) | 脂肪組織の保存方法および凍結脂肪組織からの幹細胞の分離培養方法 | |
CN116478925A (zh) | 一种体外快速制备肿瘤细胞球的方法 | |
CN114686421B (zh) | 一种肺组织脱细胞外基质微载体的制备方法及其应用 | |
CN114181896A (zh) | 一种脐带间充质干细胞营养液制备方法及其应用 | |
CN113018501A (zh) | 一种内皮祖细胞外泌体医用敷料、制备方法及其应用 | |
CN109762784B (zh) | 氯氮平在用于延缓培养的间充质干细胞衰老中的应用 | |
CN112569408B (zh) | 组织工程补片及其制备方法 | |
CN115644164B (zh) | 细胞保存液的制备方法及制备获得的细胞保存液 | |
CN107119010B (zh) | 一种用于细胞培养的合成粘附培养基及其制备方法 | |
CN117603908A (zh) | 一种msc成球培养基及培养方法 | |
CN117736975A (zh) | 一种子宫内膜干细胞的分离提取和培养方法 | |
CN117106724A (zh) | 一种h3k27m突变型脊髓胶质瘤细胞及其培养方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210819 Address after: 510300 unit 502, 5th floor, production area, No.1 helix 4 road, Guangzhou International Biological Island, Guangzhou Development Zone, Guangdong Province Applicant after: GUANGZHOU SALIAI STEM CELL SCIENCE AND TECHNOLOGY Co.,Ltd. Applicant after: GUANGZHOU SAILAILA BIOLOGICAL GENE ENGINEERING Co.,Ltd. Address before: 510000 unit 201, second floor, No. 1 production area, helix 4th Road, Huangpu District (Guangzhou International Biological Island), Guangzhou City, Guangdong Province Applicant before: Guangdong Guoke Cell Technology Co.,Ltd. Applicant before: Guangdong kangqilai precision medical Research Institute |
|
GR01 | Patent grant | ||
GR01 | Patent grant |