CN113423816A - 一种用于干细胞大规模制备的三维培养方法 - Google Patents
一种用于干细胞大规模制备的三维培养方法 Download PDFInfo
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Abstract
一种用于干细胞大规模制备的三维培养方法，包括基于三维微载体的细胞复苏方法、基于三维微载体细胞培养的原位传代方法、三维微载体原位冷冻保存细胞的方法、三维微载体细胞吸附培养的方法、收获三维微载体上的细胞的方法、对在微载体上培养的细胞进行取样的方法，和基于三维微载体的细胞大规模扩增方法。
Description
PCT国内申请，说明书已公开。
Claims (27)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910101736.0A CN109628373B (zh) | 2019-01-31 | 2019-01-31 | 一种收获三维微载体上的细胞的方法 |
| CN2019100980036 | 2019-01-31 | ||
| CN201910098003.6A CN109593704B (zh) | 2019-01-31 | 2019-01-31 | 一种三维微载体细胞吸附培养的方法 |
| CN2019100976505 | 2019-01-31 | ||
| CN201910097650.5A CN109566604B (zh) | 2019-01-31 | 2019-01-31 | 一种三维微载体原位冷冻保存细胞的方法 |
| CN2019101017360 | 2019-01-31 | ||
| CN201910476110.8A CN110218764B (zh) | 2019-06-03 | 2019-06-03 | 一种对在微载体上培养的细胞进行取样计数的方法 |
| CN2019104761108 | 2019-06-03 | ||
| CN201910505614.8A CN110229782A (zh) | 2019-06-12 | 2019-06-12 | 一种基于三维微载体细胞培养的原位传代方法 |
| CN2019105056148 | 2019-06-12 | ||
| CN2019108127732 | 2019-08-30 | ||
| CN201910812773.2A CN110452867A (zh) | 2019-08-30 | 2019-08-30 | 一种基于三维微载体细胞复苏的方法 |
| PCT/CN2019/110956 WO2020155668A1 (zh) | 2019-01-31 | 2019-10-14 | 一种用于干细胞大规模制备的三维培养方法 |
Publications (1)
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|---|---|
| CN113423816A true CN113423816A (zh) | 2021-09-21 |
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| Country | Link |
|---|---|
| US (1) | US20220162560A1 (zh) |
| EP (1) | EP3933032A4 (zh) |
| JP (1) | JP2022523129A (zh) |
| KR (1) | KR20210134323A (zh) |
| CN (1) | CN113423816A (zh) |
| WO (1) | WO2020155668A1 (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109593704A (zh) * | 2019-01-31 | 2019-04-09 | 北京华龛生物科技有限公司 | 一种三维微载体细胞吸附培养的方法 |
| CN116396930A (zh) * | 2023-06-08 | 2023-07-07 | 北京华龛生物科技有限公司 | 间充质干细胞无血清培养基及其应用 |
| CN117070474A (zh) * | 2023-10-10 | 2023-11-17 | 北京华龛生物科技有限公司 | 一种三维多孔微载体悬浮培养细胞生产病毒的方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112462076B (zh) * | 2020-11-12 | 2022-10-18 | 天津华龛生物科技有限公司 | 应用于微载体的内毒素含量的检测方法 |
| CN113376079A (zh) * | 2021-05-31 | 2021-09-10 | 上海碧博生物医药科技有限公司 | 利用CountStar Rigel系统结合细胞染色来分析转染效率的方法 |
| CN113699093B (zh) * | 2021-10-28 | 2022-03-22 | 北京华龛生物科技有限公司 | 一种保存细胞制剂的方法 |
| WO2023208212A1 (zh) * | 2022-04-29 | 2023-11-02 | 北京华龛生物科技有限公司 | 基于三维多孔微载体的病毒载体生产方法 |
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- 2019-10-14 JP JP2021544774A patent/JP2022523129A/ja active Pending
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- 2019-10-14 KR KR1020217027722A patent/KR20210134323A/ko unknown
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| CN109593704A (zh) * | 2019-01-31 | 2019-04-09 | 北京华龛生物科技有限公司 | 一种三维微载体细胞吸附培养的方法 |
| CN109593704B (zh) * | 2019-01-31 | 2022-02-11 | 北京华龛生物科技有限公司 | 一种三维微载体细胞吸附培养的方法 |
| CN116396930A (zh) * | 2023-06-08 | 2023-07-07 | 北京华龛生物科技有限公司 | 间充质干细胞无血清培养基及其应用 |
| CN116396930B (zh) * | 2023-06-08 | 2023-09-22 | 北京华龛生物科技有限公司 | 间充质干细胞无血清培养基及其应用 |
| CN117070474A (zh) * | 2023-10-10 | 2023-11-17 | 北京华龛生物科技有限公司 | 一种三维多孔微载体悬浮培养细胞生产病毒的方法 |
| CN117070474B (zh) * | 2023-10-10 | 2024-02-06 | 北京华龛生物科技有限公司 | 一种三维多孔微载体悬浮培养细胞生产病毒的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020155668A1 (zh) | 2020-08-06 |
| US20220162560A1 (en) | 2022-05-26 |
| EP3933032A1 (en) | 2022-01-05 |
| KR20210134323A (ko) | 2021-11-09 |
| JP2022523129A (ja) | 2022-04-21 |
| EP3933032A4 (en) | 2022-11-23 |
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