CN110646622B - HPV16 type E7 protein-based immunodetection device and use method - Google Patents
HPV16 type E7 protein-based immunodetection device and use method Download PDFInfo
- Publication number
- CN110646622B CN110646622B CN201910953524.5A CN201910953524A CN110646622B CN 110646622 B CN110646622 B CN 110646622B CN 201910953524 A CN201910953524 A CN 201910953524A CN 110646622 B CN110646622 B CN 110646622B
- Authority
- CN
- China
- Prior art keywords
- case
- wall
- fixedly connected
- protein
- electromagnet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Abstract
The invention discloses an immunodetection device based on HPV16 type E7 protein and a use method thereof, and the immunodetection device comprises a case, an optical light-emitting component, a control panel fixedly arranged at the top of the case and a microporous plate arranged in the case in a sliding manner through a ball guide rail, wherein the top of the inner wall of the case is fixedly provided with a signal detector, and one side of the inner wall of the case, which is positioned on the ball guide rail, is fixedly connected with an oscillation device. The immunodetection device based on the HPV16 type E7 protein and the use method thereof enable the HPV16 type E7 protein to be detected, which is stacked up and down or aggregated into a cluster in a sample, to be separated and homogenized, avoid the situation that few or wrong capture is caused when a fluorescent signal is irradiated and captured, thereby greatly reducing the error of a detection result, ensuring the accuracy of the detection result, and simultaneously realizing the adjustment of the light emission angle of an optical light-emitting component by arranging a correction device at the bottom of the optical light-emitting component.
Description
Technical Field
The invention relates to the technical field of medical detection equipment, in particular to an immunodetection device based on HPV16 type E7 protein and a use method thereof.
Background
Human Papilloma Virus (HPV) is an epitheliotropic virus consisting of 3 gene regions including Early Region (E Region), late Region (L Region) and noncoding Region (UCR) or Upstream Regulatory Region (URR), the E Region is 7 genes in the order of E6, E7, E1, E2, E3, E4 and E5, and the genes involved in replication, transcription of viral DNA, encoding viral proteins, maintaining high copy number of intracellular viruses, wherein E6 and E7 are major oncogenes of HPV, and are involved in viral cell transformation function and carcinogenicity, and the E6 and E7 proteins inactivate tumor suppressor proteins p53 and pRB, respectively, releasing cell cycle control and inhibiting apoptosis, so the best method for determining the HPV state in tumors is to measure E6/E7 protein in tumor cells.
High-risk Human Papilloma Virus (HPV) has been identified as a cause of cervical cancer, but more than 90% of HPV infection is transient and can be eliminated by the immune system within 2 years, and after HPV virus is persistently infected, especially after HPV DNA and DNA of human cervical epithelial cells are integrated, E6 and E7 genes can express a substance called mRNA in large quantity, thereby generating oncogenic proteins in large quantity and gradually cancerating human cells.
At present, when HPV16 type E7 protein in a detection sample is detected, a POCT fluorescent detection method is mostly adopted for detection, when the detection method is adopted for detection, a detection instrument is mostly directly adopted for dropwise adding the sample to be detected onto a detection plate, then an optical detection instrument is used for carrying out irradiation, signal capture and analysis, and finally a detection result is obtained, however, the existing detection method has the following defects;
1. when the detector is used for detection, after a sample is dripped on the detection plate, the detection sample has a certain concentration, and HPV16 type E7 protein to be detected in the sample is easy to be vertically stacked or aggregated into a cluster, so that less capture or mistaken capture is caused when a fluorescent signal is irradiated and captured, the error of the detection result is larger, and the accuracy of the detection result cannot be ensured.
2. Because the internal optical light-emitting component of the detector is an optical precision device, the irradiation angle of the internal optical light-emitting component of the detector is easy to deviate in the use or carrying process of the detector, and therefore, when a sample is detected, the condition that a fluorescence signal is dim or no light occurs, so that the detection result is inaccurate, or the detector cannot be normally used directly.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides an immunodetection device based on HPV16 type E7 protein and a use method thereof, which solve the problems that when a detector is used for detection, a sample is dripped on a detection plate, the detected sample has certain concentration, and the HPV16 type E7 protein to be detected in the sample is easy to be vertically stacked or aggregated, so that the detection result has larger error, the accuracy of the detection result cannot be ensured, and meanwhile, when the sample is detected, the situation that the position of an optical luminescent component is deviated, so that a fluorescent signal is dim or dark occurs, so that the detection result is inaccurate.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides an immunodetection device based on HPV16 type E7 protein, includes quick-witted case, optical lighting component, fixed mounting in the control panel of quick-witted roof portion and through ball guide slidable mounting in the micropore board of quick-witted incasement portion, quick-witted incasement wall's top fixed mounting has the signal detector, the inner wall of quick-witted case just is located one side fixedly connected with oscillation device of ball guide, oscillation device includes the box, the inner wall of box has the mounting panel through slider slidable connection, and the positive fixed mounting of mounting panel has servo motor, shaft coupling fixedly connected with two-way threaded rod is passed through to servo motor output shaft's one end, the bottom of box inner wall has the sliding plate through slider slidable connection, and the bottom of two-way threaded rod is passed through the bearing and is connected with the top rotation of sliding plate, the equal threaded connection of surface at two-way threaded rod both ends has the screw sleeve, and the equal fixedly connected with clamp plate in one side of two screw sleeves, two the equal fixedly connected with elastic rubber pad in the opposite one side of clamp plate, bolt fixedly connected with the electro-magnet of one side of box inner wall and the insulation board fixedly connected with electro-magnet that matches with the electro-magnet, the top fixedly connected with control of electro-magnet inner wall oscillator controller of box.
The bottom fixedly connected with of quick-witted incasement wall corrects the case, the bottom of correcting the incasement wall is rotated through rotating the piece and is connected with the fixed plate, and the top of fixed plate and optical light emitting component's bottom fixed connection, the both sides of fixed plate bottom all are rotated through rotating the piece and are connected with the ejector pin, and the ejector pin keeps away from the one end fixedly connected with actuating lever of fixed plate, the one end fixedly connected with ladder torsion bar of actuating lever, and the one end of ladder torsion bar is connected with the back face rotation of correcting the incasement wall through the bearing, the other end of ladder torsion bar runs through correcting case and quick-witted case in proper order and extends to the outside of quick-witted case, the back of correcting the incasement wall has the limit structure with ladder torsion bar looks adaptation through gag lever post and reset spring swing joint respectively.
Preferably, the both sides of correction incasement wall bottom are the equal fixedly connected with wedge, and the top of wedge passes through one side fixed connection of auxiliary spring and ejector pin.
Preferably, limit structure includes spacing sleeve, the spacing groove with gag lever post looks adaptation is seted up to spacing sleeve's one end, and spacing sleeve's inner wall has seted up the joint groove, and the inside in joint groove is through joint spring fixedly connected with T type dop, the one end of T type dop runs through spacing sleeve and extends to spacing sleeve's inside, and the terminal surface that T type dop extends to the inside one end of spacing sleeve is 60 degrees slopes, the draw-in groove with T type dop looks adaptation is seted up to the surface of ladder torsion bar one end.
Preferably, the inner wall and the fixed mounting of machine case have the thermostat, and one side of machine incasement wall from last to down in proper order fixed mounting have microprocessor, positive correlation algorithm analysis module and signal analysis unit.
Preferably, the microprocessor is electrically connected with the signal detector, the thermostat, the control panel, the positive correlation algorithm analysis module, the signal analysis unit and the oscillation controller in a bidirectional manner through conductive wires, and the output end of the microprocessor is electrically connected with the optical light-emitting assembly and the input end of the servo motor through conductive wires.
Preferably, the signal analysis unit includes a single group of metering modules, a single group of signal analysis modules, a plurality of groups of signal integration modules, a plurality of groups of data analysis modules, an overall weight analysis module, and an analysis result sending module.
Preferably, the sliding grooves matched with the ball guide rails are formed in the two sides of the microporous plate.
The invention also discloses a use method of the immunodetection device based on the HPV16 type E7 protein, which specifically comprises the following steps:
s1, firstly, electrifying the whole device through an external power supply, then pulling out a microporous plate from a case, dropwise adding a HPV16 type E7 protein sample to be detected with a corresponding volume on the microporous plate, then pushing the microporous plate into the case, starting a thermostat to adjust the temperature in the case to a required temperature, and keeping the constant temperature;
s2, operation control panel makes the servo motor in the microprocessor control oscillation device carry out work, servo motor can drive two clamp plates through two-way threaded rod and threaded sleeve respectively and draw close to the centre, can press from both sides tight fixed with the micropore board, then microprocessor can control the oscillation controller and lets in the heavy current alternating current in making the electro-magnet, thereby make the electro-magnet have great magnetic force, carry out stage attraction and repulsion to magnet, thereby make the micropore board about little high frequency oscillation, after the oscillation, can control through oscillation controller control electro-magnet outage, and reset through microprocessor control servo motor reversal, thereby accomplish the oscillation processing of sample:
s3, then, a detection person can check whether the optical light-emitting component is in a vertical light-emitting state or not by watching the control panel, if the optical light-emitting component is not in the vertical light-emitting state, the position of the optical light-emitting component needs to be corrected, and at the moment, the detection person can adjust the optical light-emitting component through a correction structure in the correction box;
s4, during correction, firstly judging the inclination direction of the optical light-emitting component, then determining the correction direction, during adjustment, firstly pressing the two limiting structures inwards to separate the T-shaped chuck from the clamping groove, then rotating the left or right step torsion bar to enable the step torsion bar to drive the fixing plate to incline through the driving rod and the ejector rod respectively, when the optical light-emitting component is adjusted to a proper position, respectively loosening the two limiting structures, enabling the limiting sleeve to slide outwards under the action of the reset spring to enable the T-shaped chuck to be clamped into the clamping groove, and completing the limitation of the step torsion bar;
s5, after the adjustment of the step S3, the optical light-emitting component vertically emits light to irradiate the sample of the microporous plate, then the signal detector at the top of the microporous plate can detect the fluorescence information emitted by the sample in the microporous plate and transmit the detected information to the microprocessor, and the microprocessor can control the signal analysis unit to analyze the detected signal;
s6, a single group of metering modules in the signal analysis unit can meter the fluorescence data in a single hole groove on the detected microporous plate, then the fluorescence data is analyzed through the single group of signal analysis modules, then each single group of analyzed data information is subjected to data integration through the multiple groups of signal integration modules, then the fluorescence data is analyzed and processed through the multiple groups of data analysis modules, then the whole weight analysis module performs weight algorithm processing analysis through the weight occupied by the single group of data information and the multiple groups of weights, and finally the fluorescence data is sent to the microprocessor through the analysis result sending module;
and S7, after receiving the result analyzed in the step S6, the microprocessor sends the analysis result to a positive correlation algorithm analysis module, analyzes the positive correlation between the fluorescence signal data and the E7 protein content in the sample, draws a relation curve graph, and then displays the relation curve graph on a control panel for a tester to observe and analyze.
(III) advantageous effects
The invention provides an HPV16 type E7 protein-based immunodetection device and a using method thereof. Compared with the prior art, the method has the following beneficial effects:
(1) The immunodetection device and the use method based on HPV16 type E7 protein, one side fixedly connected with oscillation device through the inner wall at the machine case and located at ball guide, oscillation device includes the box, the inner wall of box has the mounting panel through slider sliding connection, and the front fixed mounting of mounting panel has servo motor, the one end of servo motor output shaft passes through shaft coupling fixedly connected with two-way threaded rod, the bottom of box inner wall has the sliding plate through slider sliding connection, and the bottom of two-way threaded rod is rotated through the top of bearing and sliding plate and is connected, the equal threaded connection of surface at two ends of two-way threaded rod has the screw sleeve, and the equal fixedly connected with pinch-off blades of one side of two screw sleeves, the equal fixedly mounted elastic rubber pad of one side that two pinch-off blades are relative, bolt fixedly connected with the electro-magnet through the insulation board, and one side of servo motor has the magnet of adaptation with the electro-magnet through insulation board fixedly connected with, the oscillation controller of top fixedly connected with control electro-magnet of box inner wall, can realize the internally mounted oscillation device at the detector, before detection, the sample is micro-oscillating for a period of time, make the sample in the homogeneous treatment, make the sample homogeneous detection result of the upper and the fine catching of the E16 type that the segregation of the micro-plate is detected, thereby the error of the fine detection of the misconversion detection has been carried out, the fine detection of the misconversion detection has been avoided and has been carried out, and has been reduced and can be carried out.
(2) The immune detection device based on HPV16 type E7 protein and the using method thereof are characterized in that a correction box is fixedly connected to the bottom of the inner wall of a case, the bottom of the inner wall of the correction box is rotatably connected with a fixed plate through a rotating part, the top of the fixed plate is fixedly connected with the bottom of an optical light-emitting component, two sides of the bottom of the fixed plate are rotatably connected with ejector rods through rotating parts, one ends of the ejector rods, far away from the fixed plate, are fixedly connected with driving rods, one ends of the driving rods are fixedly connected with step torsion bars, one ends of the step torsion bars are rotatably connected with the back of the inner wall of the correction box through bearings, the other ends of the step torsion bars sequentially penetrate through the correction box and the case and extend to the outside of the case, the back of the inner wall of the correction box is movably connected with limit structures matched with the step torsion bars through limit rods and reset springs respectively, the situation that no light signal or no light signal occurs when a sample is detected can be well avoided, thereby ensuring more accurate detection results and normal use of the detector.
(3) The signal analysis unit comprises a single group metering module, a single group signal analysis module, a multi-group signal integration module, a multi-group data analysis module, an integral weight analysis module and an analysis result sending module, the microprocessor is electrically connected with the positive correlation algorithm analysis module in a two-way mode, single group and multi-group integration analysis of detected signal data can be realized, the analysis result is more accurate, excessive or insufficient extreme single group sample data are well eliminated, and therefore detection errors are greatly reduced.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of the structure of the present invention;
FIG. 3 is a schematic structural diagram of an oscillating device according to the present invention;
FIG. 4 is a cross-sectional view of the calibration box structure of the present invention;
FIG. 5 is an enlarged view of a portion of the invention at A in FIG. 4;
FIG. 6 is a side view of the interior of the spacing device of the present invention;
FIG. 7 is a top view of the interior of the spacing device of the present invention;
FIG. 8 is a schematic view of a T-chuck in accordance with the present invention;
FIG. 9 is a top view of a microporous plate structure of the present invention;
fig. 10 is a schematic block diagram of the architecture of the system of the present invention.
In the figure, 1 chassis, 2 optical light emitting components, 3 control panel, 4 ball guide rails, 5 micro-porous plates, 6 signal detectors, 7 oscillation devices, 71 boxes, 72 mounting plates, 73 servo motors, 74 two-way threaded rods, 75 sliding plates, 76 threaded sleeves, 77 clamping plates, 78 elastic rubber pads, 79 electromagnets, 710 magnets, 711 oscillation controllers, 8 correction boxes, 9 fixing plates, 10 ejector rods, 11 driving rods, 12 stepped torsion bars, 13 limiting rods, 14 reset springs, 15 limiting structures, 151 limiting sleeves, 152 limiting grooves, 153 clamping grooves, 154T-shaped clamping heads, 155 clamping grooves, 16 wedge blocks, 17 thermostats, 18 microprocessors, 19 positive correlation algorithm analysis modules, 20 signal analysis units, 201 single-group metering modules, 202 single-group signal analysis modules, 203 multiple-group signal integration modules, 204 multiple-group data analysis modules, 205 overall weight analysis modules, 206 analysis result sending modules and 21 sliding grooves.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 10, an embodiment of the present invention provides a technical solution: an immunodetection device based on HPV16 type E7 protein comprises a case 1, an optical light-emitting component 2, a control panel 3 fixedly mounted at the top of the case 1 and a microporous plate 5 slidably mounted in the case 1 through a ball guide rail 4, a signal detector 6 is fixedly mounted at the top of the inner wall of the case 1, the signal detector 6 is a photoelectric detector of XARM-MPI-EII type, sliding grooves 21 matched with the ball guide rail 4 are formed in two sides of the microporous plate 5, an oscillation device 7 is fixedly connected to one side of the inner wall of the case 1, which is positioned on the ball guide rail 4, the oscillation device 7 comprises a case body 71, the inner wall of the case body 71 is slidably connected with a mounting plate 72 through a sliding piece, a servo motor 73 is fixedly mounted on the front surface of the mounting plate 72, one end of an output shaft of the servo motor 73 is fixedly connected with a bidirectional threaded rod 74 through a coupler, and the bottom of the inner wall of the case body 71 is slidably connected with a sliding plate 75 through a sliding piece, the bottom end of the bidirectional threaded rod 74 is rotatably connected with the top of the sliding plate 75 through a bearing, the outer surfaces of the two ends of the bidirectional threaded rod 74 are both in threaded connection with threaded sleeves 76, one side of each of the two threaded sleeves 76 is fixedly connected with a clamping plate 77, one side of each of the two clamping plates 77 is fixedly provided with an elastic rubber pad 78, one side of the inner wall of the box body 71 is fixedly connected with an electromagnet 79 through a bolt, one side of the servo motor 73 is fixedly connected with a magnet 710 matched with the electromagnet 79 through an insulating plate, the top of the inner wall of the box body 71 is fixedly connected with an oscillation controller 711 for controlling the electromagnet 79, the model of the oscillation controller 711 is NE040S, the inner wall and the thermostat 17 are fixedly arranged on the inner wall of the case 1, and the microprocessor 18, the positive correlation algorithm analysis module 19 and the signal analysis unit 20 are fixedly arranged on one side of the inner wall of the case 1 from top to bottom in sequence, the model of the microprocessor 18 is KY101-1100, the microprocessor 18 is respectively electrically connected with the signal detector 6, the thermostat 17, the control panel 3, the positive correlation algorithm analysis module 19, the signal analysis unit 20 and the oscillation controller 711 in a bidirectional manner through conducting wires, the model of the thermostat 17 is HC05, the output end of the microprocessor 18 is respectively electrically connected with the input ends of the optical light-emitting assembly 2 and the servo motor 73 through conducting wires, the signal analysis unit 20 comprises a single-group metering module 201, a single-group signal analysis module 202, a multi-group signal integration module 203, a multi-group data analysis module 204, an overall weight analysis module 205 and an analysis result sending module 206, light beams emitted by a light source in the optical light-emitting assembly 2 pass through an optical filter or a monochromator to become monochromatic light, and then reach the signal detector 6 after being partially absorbed by a sample to be detected in the plastic microporous plate 5, the signal detector 6 converts the received optical signal into an electrical signal, and after analog signal processing such as pre-amplification, logarithmic amplification, analog-to-digital conversion and the like, the electrical signal enters the microprocessor 18 for data processing and calculation, the final detection result is displayed on the control panel 3 and can be directly printed out, in the process of irradiating the micropore plate 5 with light, the instrument can automatically perform central positioning on the hole to eliminate detection errors caused by unevenness of the bottom of the hole, generally, the instrument needs to perform measurement of 35 points, then the average value of the middle 5 points is selected as the final measurement result of the hole, the light passes through the detected object, the front and back energy difference is the energy absorbed by the detected object, and under a specific wavelength, the concentration of the same detected object and the absorbed energy form a quantitative relation.
The bottom of the inner wall of the case 1 is fixedly connected with a correction case 8, the bottom of the inner wall of the correction case 8 is rotatably connected with a fixed plate 9 through a rotating member, the top of the fixed plate 9 is fixedly connected with the bottom of the optical light-emitting component 2, both sides of the bottom of the fixed plate 9 are rotatably connected with ejector rods 10 through rotating members, one end of each ejector rod 10 far away from the fixed plate 9 is fixedly connected with a driving rod 11, one end of each driving rod 11 is fixedly connected with a stepped torsion bar 12, one end of each stepped torsion bar 12 is rotatably connected with the back of the inner wall of the correction case 8 through a bearing, the other end of each stepped torsion bar 12 sequentially penetrates through the correction case 8 and the case 1 and extends to the outside of the case 1, the back of the inner wall of the correction case 8 is respectively and movably connected with a limit structure 15 matched with the stepped torsion bar 12 through a limit rod 13 and a reset spring 14, the both sides of correcting box 8 inner wall bottom all fixedly connected with wedge 16, and one side fixed connection of auxiliary spring and ejector pin 10 is passed through at the top of wedge 16, limit structure 15 includes spacing sleeve 151, spacing groove 152 with gag lever post 13 looks adaptation is seted up to the one end of spacing sleeve 151, and the inner wall of spacing sleeve 151 has seted up joint groove 153, and joint spring fixedly connected with T type dop 154 is passed through to the inside of joint groove 153, the one end of T type dop 154 runs through spacing sleeve 151 and extends to the inside of spacing sleeve 151, and the terminal surface that T type dop 154 extends to the inside one end of spacing sleeve 151 is 60 degrees slopes, draw-in groove 155 with T type dop 154 looks adaptation is seted up to the surface of ladder torsion bar 12 one end.
The invention also discloses a use method of the immunodetection device based on the HPV16 type E7 protein, which specifically comprises the following steps:
s1, firstly, electrifying the whole device through an external power supply, then pulling out a microporous plate 5 from a case 1, dropwise adding a HPV16 type E7 protein sample to be detected with a corresponding volume on the microporous plate 5, then pushing the microporous plate 5 into the case 1, starting a thermostat 17 to adjust the temperature in the case 1 to a required temperature, and keeping the constant temperature;
s2, operation control panel 3 makes the servo motor 73 in microprocessor 18 control oscillation device 7 carry out work, servo motor 73 can drive two clamp plates 77 through two-way threaded rod 74 and threaded sleeve 76 respectively and draw close to the centre, can press from both sides the micropore board 5 and press from both sides tightly fixedly, then microprocessor 18 can control oscillation controller 711 makes and lets in the electro-magnet 79 and let in heavy current alternating current, thereby make electro-magnet 79 have great magnetic force, carry out stage nature attraction and repulsion to magnet 710, thereby make micropore board 5 control little high frequency oscillation about carrying out, after the oscillation, can control to lose electricity through oscillation controller 711 control electro-magnet 79, and reset through microprocessor 18 control servo motor 73 reversal, thereby accomplish the oscillation processing of sample:
s3, then, a detection person can check whether the optical light-emitting component 2 is in a vertical light-emitting state or not by watching the control panel 3, if the optical light-emitting component is not in the vertical light-emitting state, the position of the optical light-emitting component 2 needs to be corrected, and at the moment, the detection person can adjust the position through a correction structure in the correction box 8;
s4, during correction, firstly, judging the inclination direction of the optical light-emitting component 2, then determining the correction direction, during adjustment, firstly pressing the two limiting structures 15 inwards to separate the T-shaped clamping head 154 from the clamping groove 155, then rotating the left or right stepped torsion bar 12 to enable the stepped torsion bar 12 to drive the fixing plate 9 to incline through the driving rod 11 and the ejector rod 10 respectively, when the optical light-emitting component 2 is adjusted to a proper position, loosening the two limiting structures 15 respectively, enabling the limiting sleeve 151 to slide outwards under the action of the reset spring 14 to enable the T-shaped clamping head 154 to be clamped in the clamping groove 155, and completing the limiting of the stepped torsion bar 12;
s5, after the adjustment of the step S3, the optical light-emitting component 2 emits light vertically to irradiate the sample of the microporous plate 5, then the signal detector 6 at the top of the microporous plate 5 detects the fluorescence information emitted by the sample in the microporous plate 5 and transmits the detected information to the microprocessor 18, and the microprocessor 18 controls the signal analysis unit 20 to analyze the detected signal;
s6, the single-group metering module 201 in the signal analysis unit 20 meters the detected fluorescence data in a single hole groove on the micropore plate 5, then the fluorescence data is analyzed through the single-group signal analysis module 202, then the data integration is carried out on each single-group analyzed data information through the multiple-group signal integration module 203, then the analysis processing is carried out through the multiple-group data analysis module 204, then the whole weight analysis module 205 carries out weight algorithm processing analysis through the weight occupied by the single-group data information and the multiple-group weight, and finally the fluorescence data is sent to the microprocessor 18 through the analysis result sending module 206;
and S7, after receiving the analysis result in the step S6, the microprocessor 18 sends the analysis result to the positive correlation algorithm analysis module 19, analyzes the positive correlation between the fluorescence signal data and the E7 protein content in the sample, draws a relation curve graph, and then displays the relation curve graph on the control panel 3 for the detection personnel to observe and analyze.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides an immunodetection device based on HPV16 type E7 protein, includes quick-witted case (1), optical lighting component (2), fixed mounting in control panel (3) at quick-witted case (1) top and through ball guide (4) slidable mounting in the inside micropore board (5) of quick-witted case (1), the top fixed mounting of quick-witted case (1) inner wall has signal detector (6), its characterized in that: the vibration control device comprises a vibration device (7) fixedly connected to one side of the inner wall of the case (1) and located on a ball guide rail (4), the vibration device (7) comprises a case body (71), the inner wall of the case body (71) is connected with a mounting plate (72) in a sliding mode through a sliding part, a servo motor (73) is fixedly mounted on the front face of the mounting plate (72), one end of an output shaft of the servo motor (73) is fixedly connected with a bidirectional threaded rod (74) through a coupler, the bottom of the inner wall of the case body (71) is connected with a sliding plate (75) in a sliding mode through the sliding part, the bottom end of the bidirectional threaded rod (74) is rotatably connected with the top of the sliding plate (75) through a bearing, threaded sleeves (76) are in threaded connection with the outer surfaces of two ends of the bidirectional threaded rod (74), clamping plates (77) are fixedly connected to one side of each threaded sleeve (76), elastic rubber pads (78) are fixedly mounted on the opposite sides of each clamping plates (77), an electromagnet (79) is fixedly connected to one side of the inner wall of the case body (71) through a bolt, an electromagnet (79) is fixedly connected to one side of the servo motor (73), a magnet (710) matched with the electromagnet (79) through an insulating plate is fixedly connected to the electromagnet (711) which is fixedly connected to the electromagnet (79) through an electromagnet (71), and the top of the electromagnet (711) is fixedly connected to the electromagnet (711) of the inner wall of the electromagnet (79);
the bottom fixedly connected with of quick-witted case (1) inner wall corrects case (8), the bottom of correcting case (8) inner wall rotates through rotating and is connected with fixed plate (9), and the top of fixed plate (9) and the bottom fixed connection of optics light-emitting component (2), the both sides of fixed plate (9) bottom all rotate through rotating and are connected with ejector pin (10), and ejector pin (10) keep away from one end fixedly connected with actuating lever (11) of fixed plate (9), the one end fixedly connected with ladder torsion bar (12) of actuating lever (11), and the one end of ladder torsion bar (12) is passed through the bearing and is connected with the back of correcting case (8) inner wall and rotate, the other end of ladder torsion bar (12) runs through correcting case (8) and quick-witted case (1) in proper order and extend to the outside of quick-witted case (1), the back of correcting case (8) inner wall has limit structure (15) with ladder torsion bar (12) looks adaptation through gag lever (13) and reset spring (14) swing joint respectively.
2. The HPV type 16E 7 protein-based immunodetection device of claim 1, wherein: the two sides of the bottom of the inner wall of the correction box (8) are fixedly connected with wedge blocks (16), and the tops of the wedge blocks (16) are fixedly connected with one side of the ejector rod (10) through auxiliary springs.
3. The HPV type 16E 7 protein-based immunodetection device of claim 1, wherein: limiting structure (15) include stop sleeve (151), spacing groove (152) with gag lever post (13) looks adaptation is seted up to the one end of stop sleeve (151), and the inner wall of stop sleeve (151) has seted up joint groove (153), and the inside of joint groove (153) is through joint spring fixedly connected with T type dop (154), the one end of T type dop (154) is run through stop sleeve (151) and is extended to the inside of stop sleeve (151), and the terminal surface that T type dop (154) extended to the inside one end of stop sleeve (151) is 60 degrees slopes, draw-in groove (155) with T type dop (154) looks adaptation are seted up to the surface of ladder torsion bar (12) one end.
4. The HPV type 16E 7 protein-based immunodetection device of claim 1, wherein: the inner wall and the fixed mounting of machine case (1) have thermostat (17), and one side of machine case (1) inner wall from last to down in proper order fixed mounting have microprocessor (18), positive correlation algorithm analysis module (19) and signal analysis unit (20).
5. The immunoassay device according to claim 4, wherein the means for detecting the presence of HPV type 16E 7 protein comprises: the microprocessor (18) is respectively electrically connected with the signal detector (6), the thermostat (17), the control panel (3), the positive correlation algorithm analysis module (19), the signal analysis unit (20) and the oscillation controller (711) in a bidirectional mode through conducting wires, and the output end of the microprocessor (18) is respectively electrically connected with the optical light-emitting component (2) and the input end of the servo motor (73) through conducting wires.
6. The immunoassay device according to claim 4, wherein the means for detecting the presence of HPV type 16E 7 protein comprises: the signal analysis unit (20) comprises a single-group metering module (201), a single-group signal analysis module (202), a plurality of groups of signal integration modules (203), a plurality of groups of data analysis modules (204), an overall weight analysis module (205) and an analysis result sending module (206).
7. The immunoassay device according to claim 1, wherein the HPV type 16E 7 protein-based immunoassay device comprises: the both sides of micropore board (5) all seted up with ball guide (4) looks adaptation spout (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910953524.5A CN110646622B (en) | 2019-10-09 | 2019-10-09 | HPV16 type E7 protein-based immunodetection device and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910953524.5A CN110646622B (en) | 2019-10-09 | 2019-10-09 | HPV16 type E7 protein-based immunodetection device and use method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110646622A CN110646622A (en) | 2020-01-03 |
| CN110646622B true CN110646622B (en) | 2022-12-06 |
Family
ID=69012407
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910953524.5A Active CN110646622B (en) | 2019-10-09 | 2019-10-09 | HPV16 type E7 protein-based immunodetection device and use method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110646622B (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5657118A (en) * | 1996-01-23 | 1997-08-12 | Lee; John T. S. | Device and method for detection/measurement of light |
| JP3985562B2 (en) * | 2002-02-25 | 2007-10-03 | 株式会社日立製作所 | Optical head and optical disk apparatus |
| WO2012059786A1 (en) * | 2010-11-03 | 2012-05-10 | Reametrix Inc. | Measurement system for fluorescent detection, and method therefor |
| CN104297505A (en) * | 2014-11-04 | 2015-01-21 | 石家庄康普生科技有限公司 | High-capacity and high-speed quasi automatic chemiluminiscence immunoassay instrument |
| CN107525924B (en) * | 2017-08-28 | 2019-04-23 | 青岛瑞思德生物科技有限公司 | A kind of autoimmune hepatic diseases antibody assay kit |
-
2019
- 2019-10-09 CN CN201910953524.5A patent/CN110646622B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110646622A (en) | 2020-01-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8891729B2 (en) | X-ray analyzer and X-ray analysis method | |
| US8570495B2 (en) | Whole blood immunity measuring device and whole blood immunity measuring method | |
| CN107064543B (en) | Muzzle velocity measuring device based on X-ray | |
| CN106310955B (en) | A kind of detection method and its detection device of filter membrane thickness | |
| CN202614177U (en) | Comprehensive detection device of silicon wafer | |
| CN105548128A (en) | Method and device for detecting chlorophyll of coastal zone water body in situ through double optical path method | |
| JP2006098233A5 (en) | ||
| CN110646622B (en) | HPV16 type E7 protein-based immunodetection device and use method | |
| CN105699347B (en) | A kind of method that fluorescence immune chromatography readout instrument range is adjusted | |
| CN116202428A (en) | Laser measurement deviation correcting sensor and application thereof | |
| CN109507714B (en) | Method for rapidly judging gain state of detector | |
| CN209102713U (en) | A kind of moisture in coal intelligent detection device and coal property test system | |
| CN204389412U (en) | A kind of X fluorescence spectrum analyzes automatic calibration device | |
| CN207717626U (en) | A kind of fluorescence immune chromatography analyzer | |
| CN204008454U (en) | Portable near infrared spectrometer for detection of mould index in storage paddy | |
| CN108828431A (en) | A kind of three road V/F conversion circuit test macros based on LabVIEW | |
| CN212964605U (en) | Urine color turbidity specific gravity conductivity analysis device | |
| CN201368848Y (en) | AC/DC Zeeman Effect atomic absorption background correction system | |
| CN210604386U (en) | Automatic detection device for response characteristics of environmental parameters of fluorescent probe | |
| CN106225919A (en) | Distributed luminosity measuring method based on lock-in amplifier and the system of measurement | |
| CN208818340U (en) | A kind of portable type UV energy meter calibrating fixture | |
| CN113721007A (en) | Calibration method and device for rapid screening type alcohol detector | |
| JPS59182341A (en) | Apparatus for measuring anisotropy of sample luminescence | |
| CN202393698U (en) | Optical system for full-automatic biochemical analyzer | |
| CN107589083A (en) | Lead and cadmium elements are the same as the Atomic Absorption Spectrometer and detection method surveyed in a kind of grain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |