CN110646622A - Immunodetection device based on HPV16 type E7 protein and use method - Google Patents
Immunodetection device based on HPV16 type E7 protein and use method Download PDFInfo
- Publication number
- CN110646622A CN110646622A CN201910953524.5A CN201910953524A CN110646622A CN 110646622 A CN110646622 A CN 110646622A CN 201910953524 A CN201910953524 A CN 201910953524A CN 110646622 A CN110646622 A CN 110646622A
- Authority
- CN
- China
- Prior art keywords
- case
- wall
- plate
- protein
- fixedly connected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Abstract
The invention discloses an immunodetection device based on HPV16 type E7 protein and a using method thereof, and the immunodetection device comprises a case, an optical light-emitting component, a control panel fixedly arranged at the top of the case and a microporous plate arranged in the case in a sliding manner through a ball guide rail, wherein the top of the inner wall of the case is fixedly provided with a signal detector, and one side of the inner wall of the case, which is positioned on the ball guide rail, is fixedly connected with an oscillation device. The immunodetection device based on the HPV16 type E7 protein and the use method thereof enable the HPV16 type E7 protein to be detected, which is stacked up and down or aggregated into a cluster in a sample, to be separated and homogenized, avoid the situation that few or wrong capture is caused when a fluorescent signal is irradiated and captured, thereby greatly reducing the error of a detection result, ensuring the accuracy of the detection result, and simultaneously realizing the adjustment of the light emission angle of an optical light-emitting component by arranging a correction device at the bottom of the optical light-emitting component.
Description
Technical Field
The invention relates to the technical field of medical detection equipment, in particular to an immunodetection device based on HPV16 type E7 protein and a using method thereof.
Background
Human Papilloma Virus (HPV) is an epitheliotropic virus consisting of 3 gene regions including Early Region (E Region), Late Region (L Region) and noncoding Region (UCR) or Upstream Regulatory Region (URR), the E Region being 7 genes in the order of E6, E7, E1, E2, E3, E4 and E5, involved in replication, transcription of viral DNA, encoding viral proteins, maintaining high copy number of viruses within cells, wherein E6 and E7 are major oncogenes of HPV, associated with viral cell transformation function and carcinogenicity, and the E6 and E7 proteins inactivate tumor suppressor proteins p53 and pRB, respectively, releasing cell cycle control and inhibiting apoptosis, and thus the best method for determining the HPV state in tumors is to measure E6/E7 protein in tumor cells.
High-risk Human Papilloma Virus (HPV) has been identified as a cause of cervical cancer, but more than 90% of HPV infection is transient and can be eliminated by the immune system within 2 years, and after HPV virus is persistently infected, especially after HPV DNA and human cervical epithelial cell DNA are integrated, E6 and E7 genes can express a substance called mRNA in a large amount, so that a large amount of oncogenic protein is produced, and human cells are gradually cancerized.
At present, when HPV16 type E7 protein in a detection sample is detected, a POCT fluorescence detection method is mostly adopted for detection, when the detection method is adopted for detection, a detection instrument is mostly directly adopted for dropping the sample to be detected on a detection plate, then an optical detection instrument is used for carrying out irradiation, signal capture and analysis, and finally a detection result is obtained, however, the existing detection method has the following defects;
1. when the detector is used for detection, after a sample is dripped on the detection plate, the detection sample has a certain concentration, and HPV16 type E7 protein to be detected in the sample is easy to be vertically stacked or aggregated into a cluster, so that less capture or mistaken capture is caused when a fluorescent signal is irradiated and captured, the error of the detection result is larger, and the accuracy of the detection result cannot be ensured.
2. Because the detector is optical precision equipment in the internal optical light-emitting component, the irradiation angle of the internal optical light-emitting component is easy to deviate in the using or carrying process of the detector, so that the condition of dim fluorescent signals or no light occurs when a sample is detected, and the detection result is inaccurate or the detector cannot be normally used directly.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides an immunodetection device based on HPV16 type E7 protein and a use method thereof, and solves the problems that when a detector is used for detection, a sample is dripped on a detection plate, the detected sample has certain concentration, and HPV16 type E7 protein to be detected in the sample is easy to be vertically stacked or aggregated, so that the detection result has larger error, the accuracy of the detection result cannot be ensured, and meanwhile, when the sample is detected, the situation that a fluorescent signal is dim or dark due to the position deviation of an optical light-emitting component is easy to occur, so that the detection result is inaccurate.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: an immunodetection device based on HPV16 type E7 protein comprises a case, an optical light-emitting component, a control panel fixedly installed at the top of the case and a microporous plate slidably installed in the case through a ball guide rail, wherein a signal detector is fixedly installed at the top of the inner wall of the case, an oscillation device is fixedly connected to one side of the inner wall of the case, which is located on the ball guide rail, and comprises a case body, an installation plate is slidably connected to the inner wall of the case body through a sliding piece, a servo motor is fixedly installed on the front surface of the installation plate, one end of an output shaft of the servo motor is fixedly connected with a bidirectional threaded rod through a coupler, a sliding plate is slidably connected to the bottom of the inner wall of the case body through a sliding piece, the bottom end of the bidirectional threaded rod is rotatably connected with, and the equal fixedly connected with clamp plate in one side of two threaded sleeves, two the equal fixed mounting in one side that the clamp plate is relative has elastic rubber pad, there is the electro-magnet one side of box inner wall through bolt fixedly connected with, and one side of servo motor passes through the magnet of insulation board fixedly connected with and electromagnet looks adaptation, the top fixedly connected with of box inner wall controls the oscillation controller of electro-magnet.
The bottom fixedly connected with of quick-witted incasement wall corrects the case, the bottom of correcting the incasement wall is rotated through rotating and is connected with the fixed plate, and the top of fixed plate and optical lighting component's bottom fixed connection, the both sides of fixed plate bottom all are rotated through rotating and are connected with the ejector pin, and the ejector pin keeps away from the one end fixedly connected with actuating lever of fixed plate, the one end fixedly connected with ladder torsion bar of actuating lever, and the one end of ladder torsion bar passes through the bearing and is connected with the back rotation of correcting the incasement wall, the other end of ladder torsion bar runs through correcting case and quick-witted case in proper order and extends to the outside of quick-witted case, the back of correcting the incasement wall has the limit structure with ladder torsion.
Preferably, the both sides of correction incasement wall bottom are the equal fixedly connected with wedge, and the top of wedge passes through one side fixed connection of auxiliary spring and ejector pin.
Preferably, limit structure includes spacing sleeve, the spacing groove with gag lever post looks adaptation is seted up to spacing sleeve's one end, and spacing sleeve's inner wall has seted up the joint groove, and the inside in joint groove is through joint spring fixedly connected with T type dop, the one end of T type dop runs through spacing sleeve and extends to spacing sleeve's inside, and the terminal surface that T type dop extends to the inside one end of spacing sleeve is 60 degrees slopes, the draw-in groove with T type dop looks adaptation is seted up to the surface of ladder torsion bar one end.
Preferably, the inner wall and the fixed mounting of machine case have the thermostat, and one side of machine case inner wall from last to down in proper order fixed mounting have microprocessor, positive correlation algorithm analysis module and signal analysis unit.
Preferably, the microprocessor is electrically connected with the signal detector, the thermostat, the control panel, the positive correlation algorithm analysis module, the signal analysis unit and the oscillation controller in a bidirectional manner through conductive wires, and the output end of the microprocessor is electrically connected with the optical light-emitting assembly and the input end of the servo motor through conductive wires.
Preferably, the signal analysis unit includes a single group metering module, a single group signal analysis module, a multiple group signal integration module, a multiple group data analysis module, an overall weight analysis module and an analysis result sending module.
Preferably, the sliding grooves matched with the ball guide rails are formed in the two sides of the microporous plate.
The invention also discloses a use method of the immunodetection device based on the HPV16 type E7 protein, which specifically comprises the following steps:
s1, firstly, electrifying the whole device through an external power supply, then pulling out the microporous plate from the case, dropwise adding a HPV16 type E7 protein sample to be detected with a corresponding volume on the microporous plate, then pushing the microporous plate into the case, starting the thermostat to adjust the temperature in the case to the required temperature, and keeping the constant temperature;
s2, operation control panel makes the servo motor in the microprocessor control oscillation device carry out work, servo motor can drive two clamp plates through two-way threaded rod and threaded sleeve respectively and draw close to the centre, can press from both sides tight fixed with the micropore board, then microprocessor can control the oscillation controller and lets in the heavy current alternating current in making the electro-magnet, thereby make the electro-magnet have great magnetic force, carry out periodic attraction and repulsion to magnet, thereby make the micropore board control small high frequency oscillation about going on, after the oscillation, can control through oscillation controller control electro-magnet outage, and reset through microprocessor control servo motor reversal, thereby accomplish the oscillation processing of sample:
s3, then, the detection personnel can check whether the optical light-emitting component is in a vertical light-emitting state by watching the control panel, if the optical light-emitting component is not in the vertical light-emitting state, the position of the optical light-emitting component needs to be corrected, and at the moment, the detection personnel can adjust the optical light-emitting component through the correction structure in the correction box;
s4, during correction, firstly, judging the inclination direction of the optical light-emitting component, then determining the correction direction, during adjustment, firstly, pressing the two limiting structures inwards to separate the T-shaped clamp head from the clamping groove, then, rotating the left or right step torsion bar to enable the step torsion bar to drive the fixing plate to incline through the driving rod and the ejector rod respectively, when the optical light-emitting component is adjusted to a proper position, respectively loosening the two limiting structures, enabling the limiting sleeve to slide outwards under the action of the reset spring to enable the T-shaped clamp head to be clamped in the clamping groove, and completing the limitation of the step torsion bar;
s5, after the adjustment of the step S3, the optical light-emitting component emits light vertically to irradiate the sample of the micropore plate, then the signal detector at the top of the micropore plate can detect the fluorescence information emitted by the sample in the micropore plate and transmit the detected information to the microprocessor, and the microprocessor can control the signal analysis unit to carry out detection signal analysis;
s6, a single group of metering modules in the signal analysis unit can meter the fluorescence data in a single hole groove on a detected microporous plate, then the fluorescence data are analyzed through the single group of signal analysis modules, then each single group of analyzed data information is subjected to data integration through the multiple groups of signal integration modules, then the fluorescence data are analyzed and processed through the multiple groups of data analysis modules, then the whole weight analysis module performs weight algorithm processing analysis through the weight occupied by the single group of data information and the multiple groups of weights, and finally the fluorescence data are sent to a microprocessor through the analysis result sending module;
and S7, after receiving the result analyzed in the step S6, the microprocessor sends the analysis result to a positive correlation algorithm analysis module, analyzes the positive correlation between the fluorescence signal data and the content of the E7 protein in the sample, draws a relation curve graph, and then displays the relation curve graph on a control panel for the inspection staff to observe and analyze.
(III) advantageous effects
The invention provides an immunodetection device based on HPV16 type E7 protein and a using method thereof. Compared with the prior art, the method has the following beneficial effects:
(1) the oscillation device comprises a box body, the inner wall of the box body is connected with a mounting plate in a sliding way through a sliding part, the front of the mounting plate is fixedly provided with a servo motor, one end of an output shaft of the servo motor is fixedly connected with a bidirectional threaded rod through a coupler, the bottom of the inner wall of the box body is connected with a sliding plate in a sliding way through a sliding part, the bottom end of the bidirectional threaded rod is rotatably connected with the top of the sliding plate through a bearing, the outer surfaces of the two ends of the bidirectional threaded rod are both in threaded connection with threaded sleeves, one side of each of the two threaded sleeves is fixedly connected with a clamping plate, one side of the opposite side of each of the two clamping plates is fixedly provided with an elastic rubber pad, one side of the inner wall of the box body is fixedly connected with an electromagnet through a bolt, and one side of the servo motor is fixedly connected with a magnet matched, the oscillation controller of the top fixedly connected with control electro-magnet of box inner wall, can realize the internally mounted oscillation device at the detector, before detecting, carry out the microvibration to the sample in the micropore board earlier for a period of time, make the sample in every hole carry out the homogeneity processing, make the HPV16 type E7 protein that awaits measuring that range upon range of or gather into the group in the sample separate the homogeneity, fine having avoided when shining and catching fluorescence signal, can cause the condition emergence of catching less or mistake catching, thereby greatly reduced the error of testing result, fine assurance the accuracy of testing result.
(2) The bottom of the inner wall of the case is fixedly connected with a correction case, the bottom of the inner wall of the correction case is rotatably connected with a fixed plate through a rotating part, the top of the fixed plate is fixedly connected with the bottom of the optical light-emitting component, two sides of the bottom of the fixed plate are rotatably connected with ejector rods through the rotating part, one end of each ejector rod, far away from the fixed plate, is fixedly connected with a driving rod, one end of each driving rod is fixedly connected with a step torsion rod, one end of each step torsion rod is rotatably connected with the back surface of the inner wall of the correction case through a bearing, the other end of each step torsion rod sequentially penetrates through the correction case and the case and extends to the outside of the case, the back surface of the inner wall of the correction case is respectively and movably connected with a limiting structure matched with the step torsion rods through a limiting rod and a reset spring, the light emission angle of the optical light-emitting component is adjusted, even if the detector is used or carried, the irradiation angle of the internal optical light-emitting component deviates, the position can be well adjusted, the situation that a fluorescent signal is dim or no light occurs when a sample is detected is well avoided, so that the detection result is more accurate, and the normal use of the detector is ensured.
(3) The signal analysis unit comprises a single group metering module, a single group signal analysis module, a multiple group signal integration module, a multiple group data analysis module, an integral weight analysis module and an analysis result sending module, the microprocessor is electrically connected with the positive correlation algorithm analysis module in a two-way mode, single group and multiple group integration analysis of detected signal data can be realized, the analysis result is more accurate, excessive or too little extreme single group sample data is well eliminated, and therefore detection errors are greatly reduced.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of the structure of the present invention;
FIG. 3 is a schematic structural diagram of an oscillating device according to the present invention;
FIG. 4 is a cross-sectional view of the calibration box structure of the present invention;
FIG. 5 is an enlarged view of a portion of the invention at A in FIG. 4;
FIG. 6 is a side view of the interior of the spacing device of the present invention;
FIG. 7 is a top view of the interior of the spacing device of the present invention;
FIG. 8 is a schematic view of a T-chuck according to the present invention;
FIG. 9 is a top view of a microporous plate structure of the present invention;
fig. 10 is a schematic block diagram of the architecture of the system of the present invention.
In the figure, 1 chassis, 2 optical light emitting components, 3 control panel, 4 ball guide rails, 5 micro-porous plates, 6 signal detectors, 7 oscillation device, 71 box body, 72 mounting plate, 73 servo motor, 74 two-way threaded rod, 75 sliding plate, 76 threaded sleeve, 77 clamping plate, 78 elastic rubber pad, 79 electromagnet, 710 magnet, 711 oscillation controller, 8 correction box, 9 fixing plate, 10 ejector rod, 11 driving rod, 12 step torsion bar, 13 limit rod, 14 reset spring, 15 limit structure, 151 limit sleeve, 152 limit groove, 153 clamping groove, 154T-shaped clamping head, 155 clamping groove, 16 wedge block, 17 thermostat, 18 microprocessor, 19 positive correlation algorithm analysis module, 20 signal analysis unit, 201 single group metering module, 202 single group signal analysis module, 203 multiple group signal integration module, 204 multiple group data analysis module, 205 integral weight analysis module, 206 analysis result sending module and 21 sliding groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 10, an embodiment of the present invention provides a technical solution: an immunodetection device based on HPV16 type E7 protein comprises a case 1, an optical light-emitting component 2, a control panel 3 fixedly installed at the top of the case 1 and a microporous plate 5 slidably installed in the case 1 through a ball guide rail 4, a signal detector 6 is fixedly installed at the top of the inner wall of the case 1, the signal detector 6 is a photoelectric detector of XARM-MPI-EII type, sliding grooves 21 matched with the ball guide rail 4 are formed in two sides of the microporous plate 5, an oscillation device 7 is fixedly connected to one side of the inner wall of the case 1 and located on the ball guide rail 4, the oscillation device 7 comprises a case body 71, the inner wall of the case body 71 is slidably connected with a mounting plate 72 through a sliding piece, a servo motor 73 is fixedly installed on the front face of the mounting plate 72, one end of an output shaft of the servo motor 73 is fixedly connected with a bidirectional threaded rod 74 through a coupler, the bottom end of the bidirectional threaded rod 74 is rotatably connected with the top of the sliding plate 75 through a bearing, the outer surfaces of the two ends of the bidirectional threaded rod 74 are respectively in threaded connection with a threaded sleeve 76, one side of each of the two threaded sleeves 76 is fixedly connected with a clamping plate 77, one side, opposite to the two clamping plates 77, is fixedly provided with an elastic rubber pad 78, one side of the inner wall of the box body 71 is fixedly connected with an electromagnet 79 through a bolt, one side of the servo motor 73 is fixedly connected with a magnet 710 matched with the electromagnet 79 through an insulating plate, the top of the inner wall of the box body 71 is fixedly connected with an oscillation controller 711 for controlling the electromagnet 79, the model of the oscillation controller 711 is NE040S, the inner wall and the fixed thermostat 17 are arranged on the cabinet 1, the microprocessor 18, the positive correlation algorithm analysis module 19 and the signal analysis unit 20 are fixedly arranged on one side of the inner wall of the, the microprocessor 18 is respectively electrically connected with the signal detector 6, the thermostat 17, the control panel 3, the positive correlation algorithm analysis module 19, the signal analysis unit 20 and the oscillation controller 711 in a bidirectional way through a conducting wire, the type of the thermostat 17 is HC05, the output end of the microprocessor 18 is respectively electrically connected with the input ends of the optical light-emitting component 2 and the servo motor 73 through a conducting wire, the signal analysis unit 20 comprises a single-group metering module 201, a single-group signal analysis module 202, a multi-group signal integration module 203, a multi-group data analysis module 204, an integral weight analysis module 205 and an analysis result sending module 206, light beams emitted by a light source in the optical light-emitting component 2 pass through an optical filter or a monochromator to become monochromatic light, then the monochromatic light beams reach the signal detector 6 after being absorbed by a part of a sample to be detected in the plastic micropore plate 5, and the signal detector 6 converts the, after analog signal processing such as pre-amplification, logarithmic amplification, analog-to-digital conversion and the like, the data are processed and calculated in the microprocessor 18, the final detection result is displayed on the control panel 3 and can be directly printed out, the instrument can automatically center the hole in the process of irradiating the micropore plate 5 by light to eliminate detection errors caused by unevenness of the hole bottom, generally, the instrument needs to measure 35 points, then the average value of the middle 5 points is selected as the final measurement result of the hole, the light passes through the detected object, the front and back energy difference is the energy absorbed by the detected object, and under a specific wavelength, the concentration of the same detected object and the absorbed energy form a quantitative relation.
The bottom of the inner wall of the case 1 is fixedly connected with a correction case 8, the bottom of the inner wall of the correction case 8 is rotatably connected with a fixed plate 9 through a rotating member, the top of the fixed plate 9 is fixedly connected with the bottom of the optical light-emitting component 2, both sides of the bottom of the fixed plate 9 are rotatably connected with ejector rods 10 through rotating members, one end of each ejector rod 10 far away from the fixed plate 9 is fixedly connected with a driving rod 11, one end of each driving rod 11 is fixedly connected with a stepped torsion bar 12, one end of each stepped torsion bar 12 is rotatably connected with the back of the inner wall of the correction case 8 through a bearing, the other end of each stepped torsion bar 12 sequentially penetrates through the correction case 8 and the case 1 and extends to the outside of the case 1, the back of the inner wall of the correction case 8 is respectively and movably connected with a limit structure 15 matched with the, and one side fixed connection of auxiliary spring and ejector pin 10 is passed through at the top of wedge 16, limit structure 15 includes limit sleeve 151, limit slot 152 with gag lever post 13 looks adaptation is seted up to limit sleeve 151's one end, and limit sleeve 151's inner wall has seted up joint groove 153, and joint spring fixedly connected with T type dop 154 is passed through to joint groove 153's inside, the one end of T type dop 154 runs through limit sleeve 151 and extends to limit sleeve 151's inside, and the terminal surface that T type dop 154 extends to limit sleeve 151 inside one end is 60 degrees slopes, draw-in groove 155 with T type dop 154 looks adaptation is seted up to the surface of ladder torsion bar 12 one end.
The invention also discloses a use method of the immunodetection device based on the HPV16 type E7 protein, which specifically comprises the following steps:
s1, firstly, electrifying the whole device through an external power supply, then pulling out the microporous plate 5 from the cabinet 1, dropwise adding a HPV16 type E7 protein sample to be detected with a corresponding volume on the microporous plate 5, then pushing the microporous plate 5 into the cabinet 1, starting the thermostat 17 to adjust the temperature in the cabinet 1 to the required temperature, and keeping the constant temperature;
s2, operation control panel 3 makes the servo motor 73 in microprocessor 18 control oscillation device 7 work, servo motor 73 can drive two clamp plates 77 through two-way threaded rod 74 and threaded sleeve 76 respectively and draw close to the centre, can press from both sides the micropore board 5 and press from both sides fixedly, then microprocessor 18 can control oscillation controller 711 makes and lets in the electro-magnet 79 let in heavy current alternating current, thereby make electro-magnet 79 have great magnetic force, carry out stage attraction and repulsion to magnet 710, thereby make micropore board 5 control little high frequency oscillation about carrying out, after the oscillation, can control to lose the electricity through oscillation controller 711 control electro-magnet 79, and reset through microprocessor 18 control servo motor 73 reversal, thereby accomplish the oscillation processing of sample:
s3, then the inspector can check whether the optical lighting assembly 2 is in the vertical lighting state by looking at the control panel 3, if not, the position of the optical lighting assembly 2 needs to be corrected, and at this time, the inspector can adjust the position by the correction structure in the correction box 8;
s4, during correction, firstly, judging the inclination direction of the optical light-emitting component 2, then determining the correction direction, during adjustment, firstly, pressing the two limiting structures 15 inwards to separate the T-shaped clamping head 154 from the clamping groove 155, then, rotating the left or right stepped torsion bar 12 to enable the stepped torsion bar 12 to drive the fixing plate 9 to incline through the driving rod 11 and the ejector rod 10 respectively, when the optical light-emitting component 2 is adjusted to a proper position, loosening the two limiting structures 15 respectively, enabling the limiting sleeve 151 to slide outwards under the action of the reset spring 14 to enable the T-shaped clamping head 154 to be clamped in the clamping groove 155, and completing the limiting of the stepped torsion bar 12;
s5, after the adjustment of step S3, the optical light emitting device 2 emits light vertically to irradiate the sample of the microplate 5, then the signal detector 6 on the top of the microplate 5 detects the fluorescence information emitted by the sample in the microplate 5, and transmits the detected information to the microprocessor 18, and the microprocessor 18 controls the signal analyzing unit 20 to analyze the detected signal;
s6, the single-group metering module 201 in the signal analyzing unit 20 meters the detected fluorescence data in the single well on the microplate 5, analyzes the fluorescence data by the single-group signal analyzing module 202, integrates the analyzed data information by the multi-group signal integrating module 203, analyzes the fluorescence data by the multi-group data analyzing module 204, performs weight algorithm processing and analysis by the overall weight analyzing module 205 according to the weight occupied by the data information and the weights, and finally sends the fluorescence data to the microprocessor 18 by the analysis result sending module 206;
s7, after receiving the result analyzed in the step S6, the microprocessor 18 sends the analysis result to the positive correlation algorithm analysis module 19, analyzes the positive correlation between the fluorescence signal data and the E7 protein content in the sample, draws a relation curve graph, and then displays the relation curve graph on the control panel 3 for the inspection personnel to observe and analyze.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides an immunodetection device based on HPV16 type E7 protein, includes quick-witted case (1), optical lighting component (2), fixed mounting in the control panel (3) at quick-witted case (1) top and through ball guide (4) slidable mounting in the inside micropore board (5) of quick-witted case (1), the top fixed mounting of quick-witted case (1) inner wall has signal detector (6), its characterized in that: the vibration device (7) is fixedly connected to one side, located on the ball guide rail (4), of the inner wall of the case (1), the vibration device (7) comprises a case body (71), the inner wall of the case body (71) is connected with a mounting plate (72) in a sliding mode through a sliding piece, a servo motor (73) is fixedly mounted on the front face of the mounting plate (72), one end of an output shaft of the servo motor (73) is fixedly connected with a bidirectional threaded rod (74) through a coupler, the bottom of the inner wall of the case body (71) is connected with a sliding plate (75) in a sliding mode through the sliding piece, the bottom end of the bidirectional threaded rod (74) is rotatably connected with the top of the sliding plate (75) through a bearing, threaded sleeves (76) are connected to the outer surfaces of the two ends of the bidirectional threaded rod (74) in a threaded mode, clamping plates (77) are fixedly connected to one sides of the, one side of the inner wall of the box body (71) is fixedly connected with an electromagnet (79) through a bolt, one side of the servo motor (73) is fixedly connected with a magnet (710) matched with the electromagnet (79) through an insulating plate, and the top of the inner wall of the box body (71) is fixedly connected with an oscillation controller (711) for controlling the electromagnet (79);
the bottom fixedly connected with of quick-witted case (1) inner wall corrects case (8), the bottom of correcting case (8) inner wall is rotated through rotating and is connected with fixed plate (9), and the top of fixed plate (9) and the bottom fixed connection of optics light-emitting component (2), the both sides of fixed plate (9) bottom all are rotated through rotating and are connected with ejector pin (10), and ejector pin (10) keep away from one end fixedly connected with actuating lever (11) of fixed plate (9), the one end fixedly connected with ladder torsion bar (12) of actuating lever (11), and the one end of ladder torsion bar (12) is passed through the bearing and is connected with the back of correcting case (8) inner wall and rotate, the other end of ladder torsion bar (12) runs through correcting case (8) and quick-witted case (1) in proper order and extend to the outside of quick-witted case (1), the back of correcting case (8) inner wall has spacing with ladder torsion bar (12) looks adaptation through gag lever (13) and reset spring Structure (15).
2. The immunodetection device based on HPV16 type E7 protein of claim 1, wherein: the two sides of the bottom of the inner wall of the correction box (8) are fixedly connected with wedge blocks (16), and the tops of the wedge blocks (16) are fixedly connected with one side of the ejector rod (10) through auxiliary springs.
3. The immunodetection device based on HPV16 type E7 protein of claim 1, wherein: limiting structure (15) include stop sleeve (151), spacing groove (152) with gag lever post (13) looks adaptation is seted up to the one end of stop sleeve (151), and the inner wall of stop sleeve (151) has seted up joint groove (153), and the inside of joint groove (153) is through joint spring fixedly connected with T type dop (154), the one end of T type dop (154) is run through stop sleeve (151) and is extended to the inside of stop sleeve (151), and the terminal surface that T type dop (154) extended to the inside one end of stop sleeve (151) is 60 degrees slopes, draw-in groove (155) with T type dop (154) looks adaptation are seted up to the surface of ladder torsion bar (12) one end.
4. The immunodetection device based on HPV16 type E7 protein of claim 1, wherein: the inner wall and the fixed mounting of machine case (1) have thermostat (17), and one side of machine case (1) inner wall from last to down in proper order fixed mounting have microprocessor (18), positive correlation algorithm analysis module (19) and signal analysis unit (20).
5. The immunodetection device based on HPV16 type E7 protein of claim 4, wherein: the microprocessor (18) is respectively electrically connected with the signal detector (6), the thermostat (17), the control panel (3), the positive correlation algorithm analysis module (19), the signal analysis unit (20) and the oscillation controller (711) in a bidirectional mode through conducting wires, and the output end of the microprocessor (18) is respectively electrically connected with the optical light-emitting component (2) and the input end of the servo motor (73) through conducting wires.
6. The immunodetection device based on HPV16 type E7 protein of claim 4, wherein: the signal analysis unit (20) comprises a single-group metering module (201), a single-group signal analysis module (202), a plurality of groups of signal integration modules (203), a plurality of groups of data analysis modules (204), an overall weight analysis module (205) and an analysis result sending module (206).
7. The immunodetection device based on HPV16 type E7 protein of claim 1, wherein: and sliding grooves (21) matched with the ball guide rails (4) are formed in the two sides of the microporous plate (5).
8. The use method of the immunodetection device based on the HPV16 type E7 protein is characterized in that: the method specifically comprises the following steps:
s1, firstly, electrifying the whole device through an external power supply, then pulling out the microporous plate (5) from the case (1), dropwise adding a HPV16 type E7 protein sample to be detected with a corresponding volume on the microporous plate (5), then pushing the microporous plate (5) into the case (1), starting the thermostat (17) to adjust the temperature in the case (1) to the required temperature, and keeping the constant temperature;
s2, operating the control panel (3) to make the microprocessor (18) control the servo motor (73) in the oscillation device (7) to work, the servo motor (73) can drive the two clamping plates (77) to approach to the middle through the two-way threaded rod (74) and the threaded sleeve (76) respectively, the micro-porous plate (5) can be clamped and fixed, then the microprocessor (18) can control the oscillation controller (711) to lead the electromagnet (79) to be introduced with large-current alternating current, thereby leading the electromagnet (79) to have larger magnetic force, the magnet (710) is attracted and repelled in a staged way, so that the micro-porous plate (5) is oscillated in a left and right micro high frequency way, after the oscillation is finished, namely, the oscillation controller (711) can control the electromagnet (79) to lose power, and the microprocessor (18) controls the servo motor (73) to reversely reset, so that the oscillation processing of the sample is completed:
s3, then, a detector can check whether the optical light-emitting component (2) is in a vertical light-emitting state by watching the control panel (3), if the optical light-emitting component is not in the vertical light-emitting state, the position of the optical light-emitting component (2) needs to be corrected, and at the moment, the detector can adjust the position through a correction structure in the correction box (8);
s4, during correction, firstly, judging the inclination direction of the optical light-emitting component (2), then, determining the correction direction, during adjustment, firstly, pressing the two limiting structures (15) inwards to separate the T-shaped clamping head (154) from the clamping groove (155), then, rotating the step torsion bar (12) on the left side or the right side to enable the step torsion bar (12) to drive the fixing plate (9) to incline through the driving rod (11) and the ejector rod (10) respectively, when the optical light-emitting component (2) is adjusted to a proper position, loosening the two limiting structures (15) respectively, enabling the limiting sleeve (151) to slide outwards under the action of the reset spring (14) to clamp the T-shaped clamping head (154) into the clamping groove (155), and completing the limiting of the step torsion bar (12);
s5, after the adjustment of the step S3, the optical light-emitting component (2) vertically emits light to irradiate the sample of the micropore plate (5), then the signal detector (6) on the top of the micropore plate (5) can detect the fluorescence information emitted by the sample in the micropore plate (5), and transmits the detected information to the microprocessor (18), and the microprocessor (18) can control the signal analysis unit (20) to carry out detection signal analysis;
s6, a single group metering module (201) in a signal analysis unit (20) can meter fluorescence data in a single hole groove on a detected micro-porous plate (5), then the fluorescence data are analyzed through a single group signal analysis module (202), then each single group of analyzed data information is subjected to data integration through a multi-group signal integration module (203), then the analysis processing is carried out through a multi-group data analysis module (204), then the whole weight analysis module (205) carries out weight algorithm processing analysis through the weight occupied by the single group of data information and the multi-group weight, and finally the fluorescence data are sent to a microprocessor (18) through an analysis result sending module (206);
s7, after receiving the result analyzed in the step S6, the microprocessor (18) sends the analysis result to a positive correlation algorithm analysis module (19), analyzes the positive correlation between the fluorescence signal data and the content of the E7 protein in the sample, draws a relation curve graph, and then displays the relation curve graph on the control panel (3) for the inspection personnel to observe and analyze.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910953524.5A CN110646622B (en) | 2019-10-09 | 2019-10-09 | HPV16 type E7 protein-based immunodetection device and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910953524.5A CN110646622B (en) | 2019-10-09 | 2019-10-09 | HPV16 type E7 protein-based immunodetection device and use method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110646622A true CN110646622A (en) | 2020-01-03 |
| CN110646622B CN110646622B (en) | 2022-12-06 |
Family
ID=69012407
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910953524.5A Active CN110646622B (en) | 2019-10-09 | 2019-10-09 | HPV16 type E7 protein-based immunodetection device and use method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110646622B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5657118A (en) * | 1996-01-23 | 1997-08-12 | Lee; John T. S. | Device and method for detection/measurement of light |
| CN1441418A (en) * | 2002-02-25 | 2003-09-10 | 株式会社日立制作所 | Optical head and its regulating method and optical disc device using such optical head and method |
| US20120107950A1 (en) * | 2010-11-03 | 2012-05-03 | Manian Bala S | Measurement system for fluorescent detection, and method therefor |
| CN104297505A (en) * | 2014-11-04 | 2015-01-21 | 石家庄康普生科技有限公司 | High-capacity and high-speed quasi automatic chemiluminiscence immunoassay instrument |
| CN107525924A (en) * | 2017-08-28 | 2017-12-29 | 张庆丰 | A kind of autoimmune hepatic diseases antibody assay kit |
-
2019
- 2019-10-09 CN CN201910953524.5A patent/CN110646622B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5657118A (en) * | 1996-01-23 | 1997-08-12 | Lee; John T. S. | Device and method for detection/measurement of light |
| CN1441418A (en) * | 2002-02-25 | 2003-09-10 | 株式会社日立制作所 | Optical head and its regulating method and optical disc device using such optical head and method |
| US20120107950A1 (en) * | 2010-11-03 | 2012-05-03 | Manian Bala S | Measurement system for fluorescent detection, and method therefor |
| CN104297505A (en) * | 2014-11-04 | 2015-01-21 | 石家庄康普生科技有限公司 | High-capacity and high-speed quasi automatic chemiluminiscence immunoassay instrument |
| CN107525924A (en) * | 2017-08-28 | 2017-12-29 | 张庆丰 | A kind of autoimmune hepatic diseases antibody assay kit |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110646622B (en) | 2022-12-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20180066986A1 (en) | Light amount detection device, immune analyzing apparatus and charged particle beam apparatus that each use the light amount detection device | |
| CN101566591B (en) | Wavelength dispersion type x ray spectrometer | |
| US8570495B2 (en) | Whole blood immunity measuring device and whole blood immunity measuring method | |
| KR100467885B1 (en) | Image system calibration method and apparatus for analyzing coagulation reaction | |
| EP3637147B1 (en) | Gain correction apparatus and method for scintillation detector | |
| CN106310955B (en) | A kind of detection method and its detection device of filter membrane thickness | |
| CN101957451A (en) | Radiograph test equipment | |
| CN105929351B (en) | Test device and the test method of ultraviolet imager sensitivity | |
| WO2021115099A1 (en) | Multispectral ultraviolet light sensitivity measurement system and method | |
| CN201514316U (en) | Solar battery spectral response tester | |
| CN108801957B (en) | Biochemical substance detection instrument based on optical method and calibration method thereof | |
| CN110646622A (en) | Immunodetection device based on HPV16 type E7 protein and use method | |
| CN109507714B (en) | Method for rapidly judging gain state of detector | |
| CN105699347A (en) | Method for adjusting measurement range of fluorescence immunochromatography reading instrument | |
| CN204389412U (en) | A kind of X fluorescence spectrum analyzes automatic calibration device | |
| CN212964605U (en) | Urine color turbidity specific gravity conductivity analysis device | |
| CN206146837U (en) | A optics and detecting system for multichannel atomic fluorescence spectrophotometer | |
| CN100593116C (en) | X fluorescent multi-element analyser | |
| CN201368848Y (en) | AC/DC Zeeman Effect atomic absorption background correction system | |
| CN202393698U (en) | Optical system for full-automatic biochemical analyzer | |
| CN208818340U (en) | A kind of portable type UV energy meter calibrating fixture | |
| JPS59182341A (en) | Apparatus for measuring anisotropy of sample luminescence | |
| CN108107032B (en) | Atomic fluorescence spectrometer | |
| CN102519586B (en) | Irradiance meter for laboratory and method for implementing irradiance meter | |
| CN2886561Y (en) | Multi-element analyzer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |