CN108801957B - Biochemical substance detection instrument based on optical method and calibration method thereof - Google Patents
Biochemical substance detection instrument based on optical method and calibration method thereof Download PDFInfo
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- CN108801957B CN108801957B CN201810862075.9A CN201810862075A CN108801957B CN 108801957 B CN108801957 B CN 108801957B CN 201810862075 A CN201810862075 A CN 201810862075A CN 108801957 B CN108801957 B CN 108801957B
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- 239000000126 substance Substances 0.000 title claims abstract description 92
- 238000000034 method Methods 0.000 title claims abstract description 63
- 230000003287 optical effect Effects 0.000 title claims abstract description 56
- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 238000012360 testing method Methods 0.000 claims abstract description 33
- 238000002835 absorbance Methods 0.000 claims abstract description 32
- 238000001228 spectrum Methods 0.000 claims description 8
- 238000007405 data analysis Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000013307 optical fiber Substances 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 claims description 3
- 238000012795 verification Methods 0.000 abstract description 5
- 238000011056 performance test Methods 0.000 abstract description 2
- 238000012937 correction Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
Abstract
The invention discloses a biochemical substance detection instrument based on an optical method and a calibration method thereof, which belong to the technical field of biochemical substance detection, wherein the constant current size of an LED driver, the wavelength of an optical filter and adjustable gain are controlled and selected through an MCU chip; recording the no-load values under the four wavelength filters and the loaded values measured after adding the standard substances with the absorbance from small to large respectively; calculating to obtain absorbance A of the standard substance through the no-load value and the load value; and comparing and analyzing the absorbance A of the standard substance obtained by calculation with the standard absorbance of the standard substance to obtain calibration values under different wavelength filters, and directly writing the calibration values into the EEPROM of hardware. The invention makes the test result of the detecting instrument more accurate, and the time and cost are greatly reduced by using the calibration method for carrying out comprehensive performance test and verification on the detecting system relative to the formulated calibration scheme.
Description
Technical Field
The invention relates to a biochemical substance detection instrument and a calibration method thereof, in particular to an optical method-based biochemical substance detection instrument and a calibration method thereof, belonging to the technical field of biochemical substance detection.
Background
The current biochemical substance detection instrument is characterized in that due to the fact that the performance is limited or the difference exists in hardware, when the standard substance is used for detection, the detected data is found to have certain deviation compared with the result in a detection report of the standard substance by the national metering department, the deviation is not corrected in a reasonable range, and the deviation of the data is larger and only needs to be corrected.
The biochemical substance detecting instrument based on the optical method belongs to the instrument equipment of national metrological verification, because of many factors, the verification work of the detecting instrument is not smooth, therefore, the calibration is crucial to ensuring the normal operation of the biochemical substance detecting instrument and ensuring the accurate and reliable detection result, the calibration is not only for finding out whether the working state of the biochemical substance detecting instrument is normal, that is to say, the calibration is not the purpose, but also a means, through the working state of the calibration adjusting instrument, the factors which exist in the detecting instrument or influence the detection result are found out in time, the fault is eliminated, the normal operation of the instrument is ensured, at present, two main methods for calibrating the biochemical substance detecting instrument exist, one method is to detect by using standard substances, and the other method is to make a calibration scheme to carry out comprehensive performance test and verification on a detecting system.
In view of the above two calibration methods for the biochemical substance detecting apparatus, the present invention optimizes and improves the calibration of the biochemical substance detecting apparatus, and in addition, in the first solution, the calibration is performed by using the standard substance for detecting the biochemical substance detecting apparatus, and more, a calibration verification process is performed without making an actual calibration measure for the apparatus, so in order to solve the above two problems, it is necessary to provide an optical method-based biochemical substance detecting apparatus.
Disclosure of Invention
The invention mainly aims to provide an optical method-based biochemical substance detection instrument and a calibration method thereof, and solves the problems of overlong time and high input cost in the calibration process.
The aim of the invention can be achieved by adopting the following technical scheme:
the biochemical substance detection instrument based on the optical method comprises an MCU chip, an LED driver, an optical filter, a gain adjustable amplifying circuit and an ADC acquisition circuit, wherein the LED driver is used for driving LEDs, the optical filter is used for filtering light, the gain adjustable amplifying circuit is used for amplifying electric signals, the ADC acquisition circuit is used for acquiring signals, the LED driver is connected with a white light LED, the optical filter is connected with a sample test box through an optical fiber, the white light LED is connected with the sample test box through the optical filter, the sample test box is connected with the gain adjustable amplifying circuit through a photoelectric sensor, and the gain adjustable amplifying circuit is connected with the MCU chip through the ADC acquisition circuit.
Preferably, the LED driver is positioned at the emitting end of the biochemical substance detection instrument, the LED driver is driven by constant current, the maximum driving current of the LED driver is 1.3A, and the LED driving light source has two measuring ranges of 45ma and 145 ma.
Preferably, the gain adjustable amplifying circuit is located at the receiving end of the biochemical substance detecting instrument, and the gain of the gain adjustable amplifying circuit is selected to have two measuring ranges of 1 time and 5 times.
Preferably, the filter is a filter having 4 wavelengths, and the 4 wavelengths of the filter are 412nm, 520nm, 550nm and 630nm, respectively.
Preferably, the white light LED is a full spectrum white light LED, the full spectrum white light LED covers all wavelengths required for testing, and has sufficient luminous intensity at each wavelength.
Preferably, the photoelectric sensor is a photodiode, and is an OSD1226 type photodiode with high sensitivity at each wavelength required by the full-spectrum white light LED, and is used for ensuring that light at each wavelength required by the test can be effectively emitted and received.
Preferably, the photodiode converts an optical signal transmitted through the sample test box into an electrical signal, the electrical signal is amplified by the gain-adjustable amplifying circuit and then collected by the ADC collecting circuit, and the ADC collecting circuit transmits a collected result to the MCU chip for data analysis.
A calibrating method of a biochemical substance detecting instrument based on an optical method comprises the following steps:
the constant current of the LED driver, the wavelength of the optical filter and the adjustable gain of the gain adjustable amplifying circuit are controlled and selected by the MCU chip, so that the data collected by the ADC acquisition circuit are in a reasonable value;
the empty load values measured at the filters of four wavelengths of 412nm, 520nm, 550nm and 630nm and the loaded values measured after adding the standard substances with the absorbance ranging from small to large are recorded respectively;
the absorbance A of the standard substance is obtained through calculation of the test no-load value and the loading value after the standard substance is added;
and comparing and analyzing the absorbance A of the standard substance obtained by calculation with the standard absorbance of the standard substance to obtain calibration values of the standard substance with different absorbance under different wavelength filters, and then directly writing the calibration values into the EEPROM of hardware.
Furthermore, the no-load value is more than two thirds of the measuring range of the ADC acquisition circuit, and is in an unsaturated state.
Further, the absorbance a is related to the on-load value and the off-load value respectively, as shown in formula 1:
a= -lg (loaded/unloaded) (formula 1)
Wherein, the no-load value is an AD value measured without adding a standard substance sample; the load value is an AD value measured after adding a standard substance having a small to large absorbance.
The beneficial technical effects of the invention are as follows:
1. the invention provides a biochemical substance detecting instrument based on an optical method and a calibration method thereof, which are characterized in that a standard substance is used for detecting the detecting instrument, a proper optical filter is replaced, a proper light intensity is selected by a transmitting end, a proper gain is selected by a receiving end, then a corresponding test is completed, finally the data is analyzed and corrected, the correction value of the detecting instrument is written into an EEPROM, an LED driving light source of the transmitting end has two measuring ranges of 45ma and 145ma and can be subjected to program control switching, the gain of the receiving end also has two measuring ranges and can be subjected to program control switching, and the optical filter with 4 wavelengths can be subjected to program control switching, and the data is acquired and transmitted to an MCU through an ADC.
2. The calibration method of the biochemical substance detection instrument based on the optical method is an optimization improvement of the calibration process of the biochemical substance detection instrument based on the optical method by using standard substances, and in the calibration process of the biochemical substance detection instrument based on the optical method, the constant current and the variable gain amplification circuit of the driving light source are switched in a program-controlled manner, so that the data acquired by the ADC is more than two thirds of the measuring range, and the condition that a zero value is not measured under the test of the over-high absorbance of a sample, thereby the accurate result cannot be measured.
3. According to the biochemical substance detection instrument based on the optical method and the calibration method thereof, after the used standard substance optimizes and improves the calibration process of the biochemical substance detection instrument based on the optical method, the test result of the detection instrument is more accurate, and the time and the cost are greatly reduced when the calibration method is used for comprehensively testing the performance of the detection system and verifying the calibration method relative to the formulated calibration scheme.
Drawings
FIG. 1 is a schematic diagram showing the structure of a preferred embodiment of an optical-method-based biochemical substance detecting instrument according to the present invention.
Detailed Description
In order to make the technical solution of the present invention more clear and obvious to those skilled in the art, the present invention will be described in further detail with reference to examples and drawings, but the embodiments of the present invention are not limited thereto.
As shown in fig. 1, the biochemical substance detecting instrument based on the optical method provided in this embodiment includes an MCU chip, and an LED driver connected to the MCU chip for driving an LED, an optical filter for filtering light, a gain adjustable amplifying circuit for amplifying an electrical signal, and an ADC collecting circuit for collecting a signal, where the LED driver is connected to a white LED, the optical filter is connected to a sample test box through an optical fiber, the white LED is connected to the sample test box through an optical filter, the sample test box is connected to the gain adjustable amplifying circuit through a photoelectric sensor, and the gain adjustable amplifying circuit is connected to the MCU chip through an ADC collecting circuit.
In this embodiment, as shown in fig. 1, the LED driver is located at the emitting end of the biochemical substance detecting instrument, the LED driver is driven by a constant current, the maximum driving current of the LED driver is 1.3A, the LED driving light source thereof has two measuring ranges of 45ma and 145ma, the gain adjustable amplifying circuit is located at the receiving end of the biochemical substance detecting instrument, the gain of the gain adjustable amplifying circuit is selected to have two measuring ranges of 1 time and 5 times, the optical filter is an optical filter with 4 wavelengths, and the 4 wavelengths of the optical filter are 412nm, 520nm, 550nm and 630nm respectively.
In this embodiment, as shown in fig. 1, the white light LED is a full spectrum white light LED, the full spectrum white light LED covers all wavelengths required by the test, and has enough luminous intensity at each wavelength, the photoelectric sensor is a photodiode, and is an OSD1226 type photodiode with high sensitivity at each wavelength required by the full spectrum white light LED, and the photodiode is used for ensuring that light at each wavelength required by the test can be effectively transmitted and received, the photodiode converts an optical signal transmitted through the sample test box into an electrical signal, the electrical signal is amplified by the gain adjustable amplifying circuit and then is collected by the ADC collecting circuit, and the ADC collecting circuit transmits a collected result to the MCU chip for data analysis.
As shown in fig. 1, the calibration method of the biochemical substance detecting instrument based on the optical method provided in the embodiment includes the following steps:
the constant current of the LED driver, the wavelength of the optical filter and the adjustable gain of the gain adjustable amplifying circuit are controlled and selected by the MCU chip, so that the data collected by the ADC acquisition circuit are in a reasonable value;
the empty load values measured at the filters of four wavelengths of 412nm, 520nm, 550nm and 630nm and the loaded values measured after adding the standard substances with the absorbance ranging from small to large are recorded respectively;
the absorbance A of the standard substance is obtained through calculation of the test no-load value and the loading value after the standard substance is added;
and comparing and analyzing the absorbance A of the standard substance obtained by calculation with the standard absorbance of the standard substance to obtain calibration values of the standard substance with different absorbance under different wavelength filters, and then directly writing the calibration values into the EEPROM of hardware.
In this embodiment, the idle load value is more than two thirds of the measurement range of the ADC acquisition circuit, and is in an unsaturated state.
In this embodiment, the relationship between the absorbance a and the on-load value and the off-load value is shown in formula 1:
a= -lg (loaded/unloaded) (formula 1)
Wherein, the no-load value is an AD value measured without adding a standard substance sample; the load value is an AD value measured after adding a standard substance having a small to large absorbance.
In this embodiment, the calibration of the optical-method-based biochemical substance detection apparatus provided in this embodiment is described as follows:
the constant current size, the optical filter and the receiving end adjustable gain of the LED drive are controlled and switched by the MCU, so that the data collected by the ADC is in a reasonable value, the normal no-load value is more than two thirds of the measuring range of the ADC and can not be saturated, the test result with higher absorbance in the test standard substance can not be too small, even zero value appears, and the no-load value measured under the optical filters with four wavelengths of 412nm, 520nm, 550nm and 630nm and the loading value measured after the absorbance is added from small to large standard substance are recorded respectively.
In this embodiment, the principle analysis of the optical method-based biochemical substance detection apparatus provided in this embodiment is as follows:
the absorbance of the standard substance is obtained through testing the no-load value and the loaded value after the standard substance is added, the no-load value is the AD value measured without the standard substance, the loaded value is the AD value measured after the standard substance is added, the relation between the absorbance A and the loaded value and the no-load value is shown as a formula 1, then the A value is compared and analyzed with the detection report result of the standard substance by the national authority metering department, the calibration values of the standard substances with different absorbance under different wavelength filters are obtained, and then the calibration values are directly written into the EEPROM of hardware.
A= -lg (loaded/unloaded) (formula 1)
In this embodiment, the calibration method of the optical method-based biochemical substance detection apparatus provided in this embodiment is an optimization improvement of the calibration process of the optical method-based biochemical substance detection apparatus by using the standard substance, and in the calibration process of the optical method-based biochemical substance detection apparatus, the constant current and the variable gain amplification circuit of the driving light source are switched in a program-controlled manner, so that the data collected by the ADC is more than two thirds of the measurement range, and thus, under the test of the sample absorbance being too high, the zero value is not measured, and the accurate result cannot be measured.
In this embodiment, after the optimization and improvement of the calibration process of the biochemical substance detecting instrument based on the optical method by using the standard substance, the result of the testing of the detecting instrument is more accurate, and the time and the cost are greatly reduced when the calibration method is used for comprehensively testing the performance of the detecting system and verifying the calibration method relative to the calibration scheme.
In summary, in this embodiment, the optical method-based biochemical substance detecting apparatus and the calibration method thereof provided in this embodiment detect a standard substance used by the detecting apparatus, replace a suitable optical filter, select a suitable light intensity by a transmitting end and select a suitable gain by a receiving end, then complete a corresponding test, finally perform analysis and correction on the data, write a correction value of the detecting apparatus into an EEPROM, have two ranges of 45ma and 145ma for an LED driving light source of the transmitting end, perform program-controlled switching, have two ranges for gain selection by the receiving end, perform program-controlled switching by an optical filter with 4 wavelengths, and transmit the data to the MCU through ADC data acquisition.
The above description is merely a further embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art will be able to apply equivalents and modifications according to the technical solution and the concept of the present invention within the scope of the present invention disclosed in the present invention.
Claims (5)
1. The calibration method of the biochemical substance detection instrument based on the optical method is characterized in that the biochemical substance detection instrument comprises an MCU chip, an LED driver, an optical filter, a gain adjustable amplifying circuit and an ADC acquisition circuit, wherein the LED driver is used for driving LEDs, the optical filter is used for filtering light, the gain adjustable amplifying circuit is used for amplifying electric signals, the ADC acquisition circuit is used for acquiring signals, the LED driver is connected with a white light LED, the optical filter is connected with a sample test box through an optical fiber, the white light LED is connected with the sample test box through the optical filter, the sample test box is connected with the gain adjustable amplifying circuit through a photoelectric sensor, and the gain adjustable amplifying circuit is connected with the MCU chip through the ADC acquisition circuit;
the LED driver is positioned at the emitting end of the biochemical substance detection instrument, the LED driver is driven by constant current, the maximum driving current of the LED driver is 1.3A, and the LED driving light source has two measuring ranges of 45ma and 145 ma;
the gain adjustable amplifying circuit is positioned at the receiving end of the biochemical substance detecting instrument, and the gain of the gain adjustable amplifying circuit is selected to have two measuring ranges of 1 time and 5 times;
the optical filter is an optical filter with 4 wavelengths, and the 4 wavelengths of the optical filter are respectively 412nm, 520nm, 550nm and 630nm;
the calibration method comprises the following steps:
the constant current of the LED driver, the wavelength of the optical filter and the adjustable gain of the gain adjustable amplifying circuit are controlled and selected by the MCU chip, so that the data collected by the ADC acquisition circuit are in a reasonable value;
the empty load values measured at the filters of four wavelengths of 412nm, 520nm, 550nm and 630nm and the loaded values measured after adding the standard substances with the absorbance ranging from small to large are recorded respectively;
the absorbance A of the standard substance is obtained through calculation of the test no-load value and the loading value after the standard substance is added;
comparing and analyzing the absorbance A of the standard substance obtained by calculation with the standard absorbance of the standard substance to obtain calibration values of the standard substance with different absorbance under different wavelength filters, and then directly writing the calibration values into an EEPROM of hardware;
the no-load value is more than two thirds of the measuring range of the ADC acquisition circuit, and is in an unsaturated state.
2. The method of calibrating an optical biochemical substance detecting instrument according to claim 1, wherein the white light LED is a full spectrum white light LED that covers all wavelengths required for testing and has a sufficient luminous intensity at each wavelength.
3. The method according to claim 2, wherein the photosensor is a photodiode, and is an OSD1226 type photodiode having high sensitivity at each wavelength required for the full spectrum white LED, for ensuring that light of each wavelength required for the test can be effectively emitted and received.
4. The method for calibrating an optical biochemical substance detecting instrument according to claim 3, wherein the photodiode converts an optical signal transmitted through a sample test box into an electrical signal, the electrical signal is amplified by the gain-adjustable amplifying circuit and then collected by the ADC collecting circuit, and the ADC collecting circuit transmits the collected result to the MCU chip for data analysis.
5. The method for calibrating an optical-method-based biochemical substance detecting instrument according to claim 1, wherein the absorbance a is related to the on-load value and the off-load value, respectively, as shown in formula 1:
a= -lg (loaded/unloaded) (formula 1)
Wherein, the no-load value is an AD value measured without adding a standard substance sample; the load value is an AD value measured after adding a standard substance having a small to large absorbance.
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| CN109668847A (en) * | 2019-02-14 | 2019-04-23 | 杭州霆科生物科技有限公司 | A kind of residual tacheometer of agriculture with optics self-calibration function |
| CN110146477B (en) * | 2019-05-31 | 2023-08-04 | 成都博奥晶芯生物科技有限公司 | Preparation method of concentration gradient calibration chip and calibration method thereof |
| CN110879208A (en) * | 2019-12-04 | 2020-03-13 | 北京贝泰科技有限公司 | Take absorbance detection system of self calibration function |
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| CN102192889A (en) * | 2010-03-08 | 2011-09-21 | 上海富科思分析仪器有限公司 | Correction method for UV-visible absorption spectrum of fiber in-situ medicine leaching degree/releasing degree tester |
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