CN210604386U - Automatic detection device for response characteristics of environmental parameters of fluorescent probe - Google Patents
Automatic detection device for response characteristics of environmental parameters of fluorescent probe Download PDFInfo
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Abstract
The utility model belongs to the technical field of analysis and test, in particular to an automatic detection device for the environmental parameter response characteristic of a fluorescent probe; the device of the utility model comprises a fluorescent light source module for generating a light source; the fluorescent signal detection module is used for detecting a fluorescent signal corresponding to the to-be-detected liquid of the fluorescent probe; the environment parameter sensing module is used for sensing environment parameters; the environment parameter regulating and controlling module is used for regulating and controlling the environment parameters of the liquid to be detected of the fluorescent probe; the central controller is respectively connected with the fluorescent light source module, the fluorescent signal detection module, the environmental parameter sensing module, the environmental parameter regulating and controlling module and the computer; detection device accessible environmental parameter adjusting module automatic, directional regulation, maintain the environmental parameter that detecting system corresponds, the fluorescence probe response signal that different detection environmental parameters of high efficiency, automatic acquisition correspond realizes the high-efficient detection of fluorescence probe environmental parameter response characteristic.
Description
Technical Field
The utility model belongs to the technical field of the analysis and test, concretely relates to fluorescence probe environmental parameter response characteristic's automatic checkout device.
Background
The fluorescent probe has the characteristics of strong tissue penetrability, high efficiency, sensitivity, real-time and convenient detection and the like, and is widely used in the fields of food detection, environmental monitoring, biological imaging and the like. The high sensitivity of the fluorescent probe also means that the fluorescent probe is extremely easily influenced by environmental parameters (temperature, pH value and the like) of a detection system, so that the detection of the environmental parameter response characteristics of the fluorescent probe is very important for the reliability and stability of the detection result of the fluorescent probe.
At present, the fluorescence probe signal is mainly detected by a commercial fluorescence spectrophotometer, and the equipment can accurately and quickly detect the fluorescence signal generated by the fluorescence probe. However, due to the limitations of production cost, difficulty in operation and the like, commercial fluorescent signal detection equipment does not have the functions of sensing environmental parameters of a detection system in real time and automatically regulating and controlling the environmental parameters. The detection of the environmental parameter response characteristics of the fluorescent probe requires obtaining fluorescent signals of the environmental parameters at different levels, and when commercial fluorescent signal detection equipment detects the environmental parameter response characteristics of the fluorescent probe, the environmental parameters of the probe solution can only be adjusted to an expected level before the probe solution is placed in the fluorescent signal detection equipment. The corresponding disadvantages are as follows: (1) the environmental parameter level adjustment of the probe solution is carried out manually, and the efficiency is low; (2) after the probe solution with the environmental parameters adjusted to a specific level is placed into commercial detection equipment, the corresponding environmental parameters are easy to drift, and the existing equipment cannot sense the drift condition of the environmental parameters and cannot make dynamic compensation.
SUMMERY OF THE UTILITY MODEL
Environmental parameter for accurate, automatic regulation and control fluorescence probe solution, and then high-efficient detection fluorescence probe environmental parameter response characteristic, the utility model provides an automatic checkout device of fluorescence probe environmental parameter response characteristic.
The automatic detection device for the environmental parameter response characteristics of the fluorescent probe comprises a fluorescent signal detection module, a fluorescence signal detection module and a fluorescence signal detection module, wherein the fluorescent signal detection module is used for detecting a fluorescent signal of a liquid to be detected of the fluorescent probe; the environmental parameter sensing module is used for sensing target environmental parameters; the environment parameter regulating and controlling module is used for regulating and controlling the environment parameters of the liquid to be detected of the fluorescent probe; and the central controller is used for controlling the fluorescence signal detection module, the environmental parameter sensing module and the environmental parameter regulation and control module according to instructions.
The device also comprises a fluorescent light source module for generating a light source; the device also comprises a computer, and the computer is used for inputting control parameters, storing data and displaying detection results by a user.
The central controller is respectively in communication connection with the fluorescent light source module, the fluorescent signal detection module, the environmental parameter sensing module, the environmental parameter regulating and controlling module and the computer.
The fluorescence light source module consists of a fluorescence light source, a light source condenser and a spectroscope; light of a light source emitted by the fluorescent light source passes through the light source condenser and then the beam splitter and then is split into two paths of light, wherein one path of light enters the sample cell, and the other path of light enters the environmental parameter sensing module; the light source condenser lens is changed into parallel light rays, and the parallel light rays are divided into light intensity sensing light rays and fluorescence exciting light rays after passing through the light splitter lens.
The fluorescence signal detection module comprises a fluorescence light condenser, a detector probe and a detector controller (the detection controller is respectively connected with the detector probe and the central controller), fluorescence excitation light from the fluorescence light source module irradiates into a fluorescence probe to-be-detected liquid in the sample cell to generate emission fluorescence light, the emission fluorescence light enters the detector probe after passing through the fluorescence light condenser, and a detected fluorescence signal enters the detector controller through a detector data line.
The environment parameter sensing module comprises a light intensity sensor, a temperature sensor and a pH value sensor; wherein the light intensity sensor, the temperature sensor and the pH value sensor are all connected with the central controller; the light intensity sensor is used for sensing the light intensity of the light source, the temperature sensor is used for sensing the temperature of the liquid to be detected of the fluorescent probe, and the pH value sensor is used for sensing the temperature and the pH value of the liquid to be detected of the fluorescent probe.
The environment parameter regulation and control module comprises a light source intensity regulation submodule, a pH value regulation submodule, a fluorescent probe concentration regulation submodule, a stirring submodule and a temperature regulation submodule.
The light source intensity adjusting submodule comprises a light source intensity adjuster connected with the central controller, and the light source intensity adjuster is used for adjusting the current light intensity of the fluorescent light source to the parameter starting point of the light source intensity.
The pH value adjusting sub-module comprises a pH adjusting liquid pool, a pH adjusting solution, a pH adjusting liquid constant delivery pump controller and a pH adjusting liquid delivery pipe. Wherein the pH regulating liquid quantitative pump is positioned in the pH regulating liquid conveying pipe and is connected with the central controller through a pH regulating liquid quantitative pump controller, and the pH regulating liquid conveying pipe is communicated with the sample pool.
The fluorescent probe concentration regulator sub-module comprises a probe regulating solution pool, a probe regulating solution quantitative pump controller and a probe regulating solution conveying pipe; wherein the probe adjusting solution quantitative pump is positioned inside the probe adjusting solution conveying pipe and is connected with the central controller through a probe adjusting solution quantitative pump controller, and the probe adjusting solution conveying pipe is communicated with the sample cell.
The stirring submodule comprises a magnetic stirrer and a magnetic stirrer, the sample cell is arranged on the magnetic stirrer, the magnetic stirrer is connected with the central controller, and the magnetic stirrer is positioned in the sample cell.
The temperature regulation submodule comprises a heating plate, a cooling plate and a temperature controller; wherein the heating pieces are symmetrically arranged on two sides of the sample cell, the cooling pieces are symmetrically arranged on two sides of the sample cell, and the heating pieces and the cooling pieces are connected with the central controller through the temperature controller.
The fluorescent light source module, the fluorescent signal detection module, the environmental parameter sensing module and the environmental parameter regulation and control module are all arranged in the device shell capable of isolating environmental light.
The utility model has the advantages that:
the real-time perception detection system of usable environmental parameter perception module of detection device environmental parameter that corresponds to through the environmental parameter regulation module automatic, directional regulation, the environmental parameter that the maintenance detection system corresponds, thereby high-efficient, automatic fluorescent probe response signal that acquires the different detection environmental parameters and correspond, thereby realize fluorescent probe environmental parameter response characteristic's high-efficient detection. Has the advantages that:
(1) the utility model can realize automatic and directional adjustment of probe solution to different environmental parameter levels, and the automation degree of the device is high;
(2) the utility model can monitor the change condition of the link parameter level in real time in the probe fluorescence signal measuring process, and make dynamic compensation, so as to ensure that the actual environmental parameter of the probe solution is highly consistent with the set parameter value;
(3) the utility model discloses contained light source intensity, temperature, pH concentration regulation and control module, each module both can the autonomous working, can the collaborative work again, can characterize fluorescence probe's environmental parameter response characteristic comprehensively.
Drawings
FIG. 1 is a schematic structural diagram of a preferred embodiment of the apparatus for automatically detecting environmental parameter response characteristics of a fluorescent probe according to the present invention;
in the figure: 1-fluorescent light source, 2-light source condenser, 3-spectroscope, 4-central controller, 5-light source light, 6-light intensity induction light, 7-fluorescence excitation light, 8-sample cell, 9-fluorescent probe solution to be tested, 10-fluorescent light condenser, 11-detector probe, 12-detector data line, 13-detector controller, 14-emission fluorescent light, 15-light intensity sensor, 16-temperature sensor, 17-pH value sensor, 18-light source regulator, 19-pH regulating solution cell, 20-pH regulating solution, 21-pH regulating solution quantitative pump, 22-pH regulating solution quantitative pump controller, 23-pH regulating solution delivery pipe, 24-probe regulating solution cell, 25-probe regulating solution, 26-probe regulating solution quantitative pump, 27-probe regulating solution quantitative pump controller, 28-probe regulating solution conveying pipe, 29-magnetic stirrer, 30-magnetic stirrer, 31-heating sheet, 32-cooling sheet, 33-temperature controller and 34-computer.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the following description is made in conjunction with the specific embodiments and the accompanying drawings so that those skilled in the art can better understand the technical solution of the present invention.
Example 1:
the automatic detection device for the environmental parameter response characteristic of the fluorescent probe comprises a fluorescent signal detection module, a signal processing module and a signal processing module, wherein the fluorescent signal detection module is used for detecting a fluorescent signal of a liquid to be detected of the fluorescent probe; the environmental parameter sensing module is used for sensing target environmental parameters; the environment parameter regulating and controlling module is used for regulating and controlling the environment parameters of the liquid to be detected of the fluorescent probe; and the central controller is used for controlling the fluorescence signal detection module, the environmental parameter sensing module and the environmental parameter regulation and control module according to instructions.
The device also comprises a fluorescent light source module for generating a light source; the device also comprises a computer, and the computer is used for inputting control parameters, storing data and displaying detection results by a user.
The central controller is respectively in communication connection with the fluorescent light source module, the fluorescent signal detection module, the environmental parameter sensing module, the environmental parameter regulating and controlling module and the computer.
The fluorescence light source module comprises a fluorescence light source 1, a light source condenser 2 and a spectroscope 3; a light source light ray 5 emitted by the fluorescent light source 1 passes through the light source condenser 2, then passes through the spectroscope 3 and is divided into two light rays, wherein one light ray enters the sample cell 8, and the other light ray enters the environmental parameter sensing module; the light source condenser lens is changed into parallel light rays, and the parallel light rays are divided into light intensity sensing light rays and fluorescence exciting light rays after passing through the light splitter lens.
The fluorescence signal detection module comprises a fluorescence light condenser 10, a detector probe 11 and a detector controller 13; the detection controller 13 is connected to the detector probe 11 and the central controller 4, respectively. The fluorescence excitation light from the fluorescence light source module irradiates into the liquid to be detected of the fluorescence probe in the sample cell to generate emission fluorescence light, the emission fluorescence light enters the detector probe after passing through the fluorescence light condenser, and the detected fluorescence signal enters the detector controller through the detector data line.
The environment parameter sensing module comprises a light intensity sensor 15, a temperature sensor 16 and a pH value sensor 17; the light intensity sensor 15, the temperature sensor 16 and the pH value sensor 17 are connected with the central controller 4, the light intensity sensor is used for sensing the light intensity of the light source, the temperature sensor is used for sensing the temperature of the liquid to be measured of the fluorescent probe, and the pH value sensor is used for sensing the temperature and the pH value of the liquid to be measured of the fluorescent probe. The environment parameter regulation and control module comprises a light source intensity regulation submodule, a pH value regulation submodule, a fluorescent probe concentration regulation submodule, a stirring submodule and a temperature regulation submodule.
The light source intensity adjusting submodule comprises a light source intensity adjuster 18 connected with the central controller, and the light source intensity adjuster is used for adjusting the current light intensity of the fluorescent light source to the parameter starting point of the light source intensity.
The pH value adjusting sub-module comprises a pH adjusting liquid pool 19, a pH adjusting solution 20, a pH adjusting liquid quantitative pump 21, a pH adjusting liquid quantitative pump controller 22 and a pH adjusting liquid conveying pipe 23. Wherein the pH adjusting liquid quantitative pump 21 is positioned inside the pH adjusting liquid conveying pipe 23 and is connected with the central controller 4 through a pH adjusting liquid quantitative pump controller 22, and the pH adjusting liquid conveying pipe 23 is communicated with the sample cell 8.
The fluorescent probe concentration regulating submodule comprises a probe regulating solution pool 24, a probe regulating solution 25, a probe regulating solution quantitative pump controller 26 and a probe regulating solution conveying pipe 27; wherein the probe-regulated solution quantitative pump 25 is located inside the probe-regulated solution delivery pipe 27 and is connected to the central controller 4 through the probe-regulated solution quantitative pump controller 26, and the probe-regulated solution delivery pipe 27 is communicated with the sample cell 8.
The stirring submodule comprises a magnetic stirrer 29 and a magnetic stirrer 30, the sample cell 8 is arranged on the magnetic stirrer 29, the magnetic stirrer 29 is connected with the central controller 4, and the magnetic stirrer 30 is positioned in the sample cell 8.
The temperature regulation submodule comprises a heating plate 31, a cooling plate 32 and a temperature controller 33; wherein the heating plates 31 are symmetrically arranged at two sides of the sample cell 8, the cooling plates 32 are symmetrically arranged at two sides of the sample cell 8, and the heating plates 31 and the cooling plates 32 are connected with the central controller 4 through the temperature controller 33.
The fluorescent light source module, the fluorescent signal detection module, the environmental parameter sensing module and the environmental parameter regulation and control module are all arranged in the device shell capable of isolating environmental light.
Example 2:
utilize the utility model discloses a but fluorescence probe automated inspection device of fluorescence probe environmental parameter response characteristic automated inspection fluorescence probe is to specific environmental parameter, including the response characteristic of light source intensity, pH value, probe concentration, temperature, the concrete implementation process as follows:
the process for automatically detecting the response characteristic of the fluorescent probe to the light source intensity is as follows:
step 1: setting the starting point G of the light source intensity parameter by the computer 341An adjustment interval Δ G, and an adjustment number (N-1);
step 2: the central controller 4 senses the current light intensity G of the fluorescent light source 1 in real time by using the light intensity sensor 15, and automatically controls the light source regulator 18 to regulate the current light intensity G of the fluorescent light source 1 to the parameter starting point G of the light source intensity1Then, a fluorescence signal detection module is utilized to detect a fluorescence signal SG _1；
And step 3: according to the set light source intensity adjusting interval delta G and the adjusting times (N-1), the central controller 4 automatically controls the light source adjuster 18 to adjust the current light intensity G of the fluorescent light source 1 to (G) in sequence1+1*ΔG)，(G1+2*ΔG)，……，(G1+(N-2)*ΔG)，(G1+ (N-1) Δ G) and detecting the fluorescence signal SG _, corresponding to the solution 9, by the fluorescence signal detection module2，SG_3，……，SG_N-1，SG_N；
And 4, step 4: the central controller 4 sends the light intensity G1，(G1+1*ΔG)，(G1+2*ΔG)，……，(G1+(N-2)*ΔG)，(G1Fluorescence signal SG _ +(N-1). DELTA.G)1，SG_2，SG_3，……，SG_N-1，SG_NStored in computer 34 as a response characteristic of the fluorescent probe to the ambient parameter of light source intensity.
The central controller 4 adjusts the current light intensity G of the fluorescent light source 1 sensed by the light intensity sensor 15 in real time to a set light intensity G1The process of + N × Δ G (N ═ 0,1,2, … …, N-2, N-1) is: the central controller 4 compares the current light intensity G with the set light intensity G1+ n × Δ G, if G>G1+ n × Δ G, the central controller 4 controls the light source adjuster 18 to continuously decrease the current light intensity G of the fluorescent light source 1, so that | G- (G —) G1The + n Δ G) | decreases continuously until | G- (G)1+ n × Δ G) | 0, i.e., G ═ G1The light intensity of the fluorescent light source 1 is kept constant at + n × Δ G; if G ═ G1+ n × Δ G, the central controller 4 controls the light source regulator 18 to maintain the light intensity of the fluorescent light source 1 constant; if G is<G1+ n × Δ G, the central controller 4 controls the light source adjuster 18 to continuously increase the current light intensity G of the fluorescent light source 1, so that | G- (G —) G1The + n Δ G) | decreases continuously until | G- (G)1+ n × Δ G) | 0, i.e., G ═ G1+ n × Δ G keeps the light intensity of the fluorescent light source 1 constant.
The process for automatically detecting the response characteristic of the fluorescent probe to the pH value is as follows:
step 1: setting a parameter starting point H of the pH value by using the computer 341An adjustment interval Δ H, and an adjustment number (M-1);
step 2: the central controller 4 senses the current pH value H of the fluorescent probe solution 9 in real time by using the pH value sensor 17, and automatically controls the pH value adjusting submodule to adjust the current pH value of the fluorescent probe solution 9 to the parameter starting point H1Then, a fluorescence signal detection module is utilized to detect a fluorescence signal SH _ corresponding to the liquid 9 to be detected of1；
And step 3: the central controller 4 automatically controls the pH value adjusting submodule to sequentially adjust the current pH value H of the fluorescent probe solution 9 to (H)1+1*ΔH)，(H1+2*ΔH)，……，(H1+(M-2)*ΔH)，(H1+ (M-1) Δ H), and a fluorescence signal detection module is utilized to sequentially detect the fluorescence signal SH _, which corresponds to the fluorescence probe solution 9 to be detected2，SH_3，……，SH_M-1，SH_M；
And 4, step 4: the central controller 4 controls the pH value to be H1，(H1+1*ΔH)，(H1+2*ΔH)，……，(H1+(M-2)*ΔH)，(H1+ (M-1). DELTA.H) of the corresponding fluorescence signal SH _1，SH_2，SH_3，……，SH_M-1，SH_MStored in computer 34 as a response characteristic of the fluorescence probe to the environmental parameter of pH.
The central controller 4 adjusts the current pH value H of the liquid 9 to be detected of the fluorescent probe to a set pH value H1The process of + M Δ H (M ═ 0,1,2, … …, M-2, M-1) is: the central controller 4 compares the current pH value H and the set pH value H of the solution 9 to be detected of the fluorescent probe1+ m × Δ H, if H ═ H1+ m × Δ H, keeping the current pH value of the solution to be tested 9 of the fluorescent probe constant; if H is not equal to H1+ m × Δ H, the central controller 4 drives the pH adjusting liquid quantitative pump 21 through the pH adjusting liquid quantitative pump controller 22 to pump the pH adjusting solution 20 in the pH adjusting liquid pool 19 into the sample pool 8 through the pH adjusting liquid delivery pipe 23, meanwhile, the central controller 4 drives the magnetic stirrer 30 through the magnetic stirrer 29 of the stirring submodule to mix the added pH adjusting solution in the sample pool 8 with the original fluorescent probe solution 9 to be tested, and the current pH value H and the set pH value H of the fluorescent probe solution 9 to be tested, which are sensed by the pH sensor 17 in real time1+ m × Δ H, if | H- (H)1The + m Δ H) | is continuously decreased, and the pH adjusting solution 20 is continuously pumped until | H- (H) |1+ m × Δ H) | 0, i.e., H ═ H (H)1+ m × Δ H) stop pumping; if | H- (H)1If + m Δ H) | continues to increase, the pumping of the pH adjusting solution 20 is stopped and the "pH value of the solution to be measured cannot be adjusted" is displayed on the computer 34.
The process for automatically detecting the response characteristic of the fluorescent probe to the probe concentration is as follows:
step 1: setting a parameter starting point J of the probe concentration by the computer 341Adjusting the interval delta J and the adjusting times (X-1), and simultaneously inputting the volume V of the to-be-detected liquid 9 of the fluorescent probe placed in the sample cell 81And probe concentration J1And the probe concentration J of the probe adjusting solution 25tSetting the current volume V of the liquid 9 to be detected as the fluorescent probe as V1The current probe concentration J of the solution to be detected of the fluorescent probe 9 is equal to J1；
Step 2: the central controller 4 detects the concentration of the probe J by using a fluorescent signal detection module1Fluorescent probe to be detected 9 corresponding fluorescent signal SJ _1；
And step 3: the central controller 4 automatically controls the fluorescent probe concentration adjusting submodule to sequentially adjust the current probe concentration J of the fluorescent probe solution 9 to (J)1+1*ΔJ)，(J1+2*ΔJ)，……，(J1+(X-2)*ΔJ)，(J1+ (X-1) Δ J), and detecting a fluorescent signal SJ \ corresponding to the fluorescent probe solution 9 to be detected by using a fluorescent signal detection module2，SJ_3，……，SJ_X-1，SJ_X；
And 4, step 4: the central controller 4 sets the probe concentration value to J1，(J1+1*ΔJ)，(J1+2*ΔJ)，……，(J1+(X-2)*ΔJ)，(J1(X-1) Δ J) corresponding to fluorescent signal SJ _ \1，SJ_2，SJ_3，……，SJ_X-1，SJ_XStored in computer 34 as a response characteristic of the fluorescent probe to its own concentration environmental parameter.
The central controller 4 adjusts the current probe concentration J of the fluorescence probe solution 9 to be detected to the set probe concentration J1The process of + X Δ J (X ═ 0,1,2, … …, X-2, X-1) is: the computer 34 adjusts the probe concentration J of the solution 25 according to the current volume V of the solution 9 to be detected of the fluorescent probe, the current probe concentration J of the solution 9 to be detected of the fluorescent probe and the probe concentration J of the probe adjusting solution 25tCalculating to make the fluorescent probe solution 9 reach the set probe concentration J1Volume V of probe solution to be added + x Δ Ja＝(J-J1-x*ΔJ)*V/(J1-Jt+ x Δ J), and then the central controller 4 drives the probe-regulated solution quantitative pump 26 via the probe-regulated solution quantitative pump controller 27 to set the volume in the probe-regulated solution tank 24 to VaThe probe adjusting solution 25 is pumped into the sample pool 8 through the probe adjusting solution conveying pipe 28, and meanwhile, the central controller 4 drives the magnetic stirrer 30 through the magnetic stirrer 29 of the stirring submodule to uniformly mix the probe adjusting solution 25 added into the sample pool 8 with the original fluorescent probe solution to be detected 9; the computer automatically sets the current volume V of the liquid 9 to be detected of the fluorescent probe as V + VaSetting the current probe concentration J of the liquid 9 to be detected of the fluorescent probe as J1+x*ΔJ。
The process for automatically detecting the response characteristic of the fluorescent probe to the temperature is as follows:
step 1: setting the parameter starting point T of the temperature by the computer 341An adjustment interval DeltaT and an adjustment number (Y-1);
step 2: the central controller 4 senses the current temperature T of the liquid 9 to be detected of the fluorescent probe in real time by using the temperature sensor 16, automatically controls the temperature adjusting submodule to adjust the temperature of the liquid 9 to be detected of the fluorescent probe to the parameter starting point T1, and then detects the fluorescent signal ST _, corresponding to the liquid 9 to be detected of the fluorescent probe by using the fluorescent signal detecting module1；
And step 3: the central controller 4 automatically controls the temperature adjusting submodule to sequentially adjust the current temperature T of the fluorescent probe solution 9 to (T)1+1*ΔT)，(T1+2*ΔT)，……，(T1+(Y-2)*ΔT)，(T1+ (Y-1) Δ T), and detecting fluorescent signal ST _, corresponding to the solution 9 to be detected of the fluorescent probe, by using a fluorescent signal detection module2，ST_3，……，ST_Y-1，ST_Y；
And 4, step 4: the central controller 4 sets the temperature value as T1，(T1+1*ΔT)，(T1+2*ΔT)，……，(T1+(Y-2)*ΔT)，(T1Fluorescence signal ST _ +(Y-1). DELTA.T)1，ST_2，ST_3，……，ST_Y-1，ST_YStored in computer 34 as a response characteristic of the fluorescent probe to the temperature environment parameter.
The centerThe controller 4 adjusts the current temperature T of the liquid 9 to be detected of the fluorescent probe to the set temperature T1The process of + Y Δ T (Y0, 1,2, … …, Y-2, Y-1) is: the central controller 4 compares the current temperature T and the set temperature T of the liquid 9 to be detected of the fluorescent probe1+ y Δ T, if T<T1+ y Δ T, the central controller 4 controls the heating plate 31 to start working through the temperature controller 33, so that | T- (T —) T1+ y Δ T) l decreases continuously until T- (T)1+ y Δ T) | 0, i.e., T ═ T (T ═1+ y Δ T) to keep the current temperature T of the solution to be measured 9 of the fluorescent probe constant; if T>T1+ y Δ T, the central controller 4 controls the cooling fins 32 to start operating through the temperature controller 33, so that | T- (T —) is enabled1+ y Δ T) l decreases continuously until T- (T)1+ y Δ T) | 0, i.e., T ═ T (T ═1+ y Δ T) to keep the current temperature T of the solution to be measured 9 of the fluorescent probe constant; if T ═ T1And + y Δ T, neither the heating plate 31 nor the cooling plate 32 is operated.
Example 3:
taking the current temperature of the liquid to be detected of the fluorescent probe as 20 ℃ as an example, the device is used for automatically detecting the response characteristic of the copper nanocluster fluorescent probe to temperature environment parameters, and the specific implementation process is as follows:
step 1: setting the parameter starting point of the temperature to be 0 ℃, the adjusting interval to be 5 ℃ and the adjusting times to be 8 times by using the computer 34;
step 2: the central controller 4 senses that the current temperature of the copper nanocluster fluorescent probe liquid 9 to be detected is 20 ℃ in real time by using the temperature sensor 16, automatically controls the temperature regulator sub-module to regulate the temperature of the copper nanocluster fluorescent probe liquid 9 to be detected to be 0 ℃ of a parameter starting point, and then detects that a fluorescent signal corresponding to the copper nanocluster fluorescent probe liquid 9 is 750 by using the fluorescent signal detection module;
and step 3: the central controller 4 automatically controls the temperature adjusting sub-module to adjust the temperature of the copper nanocluster fluorescent probe liquid 9 to 5, 10, 15, 20, 25, 30, 35 and 40 ℃ in sequence, and the fluorescence signal detection module is used for detecting the corresponding fluorescence signals of the copper nanocluster fluorescent probe liquid 9 to be 748, 745, 740, 734, 725, 713, 704 and 700 respectively.
And 4, step 4: the central controller 4 stores the fluorescence signals 750, 748, 745, 740, 734, 725, 713, 704, 700 corresponding to the temperature values of 0, 5, 10, 15, 20, 25, 30, 35, 40 ℃ into the computer 34 as the response characteristics of the copper nanocluster fluorescence probe to the temperature environment parameters.
The process that the central controller 4 adjusts the current temperature of the copper nanocluster fluorescent probe solution 9 to be detected to be 20 ℃ to the set temperature of 25 ℃ is as follows: the central controller 4 compares the current temperature of 20 ℃ of the copper nanocluster fluorescent probe liquid to be detected 9 with the set temperature of 25 ℃, and when the current temperature of 20 ℃ is lower than the set temperature of 25 ℃, the central controller 4 controls the heating plate 31 to start working through the temperature controller 33, so that the absolute value of the current temperature-the set temperature is continuously reduced, and the current temperature of 25 ℃ of the copper nanocluster fluorescent probe liquid to be detected 9 is kept constant until the absolute value of the current temperature-the set temperature is 0, namely the current temperature is equal to the set temperature;
the process that the central controller 4 adjusts the current temperature of the copper nanocluster fluorescent probe solution 9 to be detected to be 20 ℃ to the set temperature of 15 ℃ is as follows: the central controller 4 compares the current temperature 20 ℃ of the copper nanocluster fluorescent probe liquid 9 to be detected with the set temperature 15 ℃, and when the current temperature 20 ℃ is higher than the set temperature, the central controller 4 controls the cooling fin 32 to start working through the temperature controller 33, so that the absolute value of the current temperature-the set temperature is continuously reduced, and the current temperature 15 ℃ of the fluorescent probe liquid 9 is kept constant until the absolute value of the current temperature-the set temperature is 0, namely the current temperature is equal to the set temperature;
the process that the central controller 4 adjusts the current temperature of the copper nanocluster fluorescent probe solution 9 to be detected to be 20 ℃ to the set temperature of 20 ℃ is as follows: the central controller 4 compares the current temperature 20 ℃ of the copper nanocluster fluorescent probe solution 9 to be detected with the set temperature 20 ℃, and if the current temperature is equal to the set temperature, the heating plate 31 and the cooling plate 32 do not work.
Example 4:
utilize the utility model discloses an automatic checkout device of fluorescence probe environmental parameter response characteristic can detect the response characteristic of fluorescence probe to light source intensity, pH value, probe concentration, temperature simultaneously, and the concrete implementation process is as follows:
setting the starting point G of the light source intensity parameter by the computer 341Light source intensity adjusting interval delta G, light source intensity adjusting times (N-1) and pH value parameter starting point H1pH value adjusting interval delta H, pH value adjusting times (M-1) and probe concentration parameter starting point J1A probe concentration regulation interval delta J, a probe concentration regulation frequency (X-1), and a volume V of a fluorescent probe solution 9 to be tested placed in the sample cell 81Probe concentration J1And the probe concentration Jt and the temperature parameter starting point T of the probe adjusting solution 251Temperature adjustment interval DeltaT and temperature adjustment times (Y-1).
The automatic detection apparatus automatically detects the response characteristics of the fluorescent probe to the light source intensity environmental parameters according to the procedure of "process of automatically detecting the response characteristics of the fluorescent probe to the light source intensity" in example 2.
The automatic detection device automatically detects the response characteristic of the fluorescent probe to the pH environmental parameter according to the procedure of automatically detecting the response characteristic of the fluorescent probe to the pH value in embodiment 2, and records and stores the volume of the pH value adjusting solution pumped into the sample cell 8, which is denoted as Vph。
Volume V of pH-value-adjusted liquid pumped into sample cell 8phCalculating the current volume V of the liquid 9 to be detected of the fluorescent probe as V1+VphThe current probe concentration J of the solution to be detected of the fluorescent probe 9 is equal to V1*J1/(V1+Vph) The automatic detection apparatus automatically detects the response characteristic of the fluorescent probe to its own concentration environmental parameter according to the procedure of "process of automatically detecting the response characteristic of the fluorescent probe to the probe concentration" in embodiment 2.
The automatic detection apparatus automatically detects the response characteristics of the fluorescent probe to the temperature environment parameters according to the procedure of "process of automatically detecting the response characteristics of the fluorescent probe to the temperature" in example 2.
Claims (9)
1. The automatic detection device of the environmental parameter response characteristic of the fluorescent probe is characterized by comprising:
the fluorescent signal detection module is used for detecting a fluorescent signal of the liquid to be detected of the fluorescent probe;
the environmental parameter sensing module is used for sensing target environmental parameters;
the environment parameter regulating and controlling module is used for regulating and controlling the environment parameters of the liquid to be detected of the fluorescent probe; and
and the central controller is used for controlling the fluorescence signal detection module, the environmental parameter sensing module and the environmental parameter regulation and control module according to instructions.
2. The apparatus for automatic detection of the environmental parameter response characteristic of a fluorescent probe according to claim 1, further comprising a fluorescent light source module for generating a light source;
the device also comprises a computer, and the computer is used for inputting control parameters, storing data and displaying detection results by a user.
3. The apparatus of claim 2, wherein the central controller is communicatively connected to the fluorescent light source module, the fluorescent signal detection module, the environmental parameter sensing module, the environmental parameter control module, and the computer (34), respectively.
4. The apparatus for automatically detecting the environmental parameter response characteristics of a fluorescent probe according to claim 2, wherein the fluorescent light source module comprises a fluorescent light source (1), a light source condenser (2) and a spectroscope (3); light source light (5) emitted by the fluorescent light source (1) passes through the light source condenser (2) and then passes through the spectroscope (3) to be divided into two paths of light, wherein one path of light enters the sample cell (8), and the other path of light enters the environmental parameter sensing module.
5. The apparatus for automatically detecting the environmental parameter response characteristic of a fluorescent probe according to claim 1, wherein the fluorescent signal detection module comprises a fluorescent light condenser (10), a detector probe (11), a detector controller (13); the detector controller (13) is respectively connected with the detector probe (11) and the central controller (4).
6. The apparatus for automatically detecting the environmental parameter response characteristic of a fluorescent probe as claimed in claim 1, wherein the environmental parameter sensing module comprises a light intensity sensor (15), a temperature sensor (16) and a pH sensor (17); light intensity inductor (15), temperature sensor (16), pH value sensor (17) all link to each other with central controller (4), light intensity inductor is used for perception light source light intensity, and temperature sensor is used for the temperature of perception fluorescence probe liquid of waiting to be measured, and pH value sensor is used for the temperature pH value of perception fluorescence probe liquid of waiting to be measured.
7. The apparatus according to claim 1, wherein the environment parameter adjusting and controlling module comprises a light source intensity adjusting sub-module, a pH value adjusting sub-module, a fluorescent probe concentration adjusting sub-module and a temperature adjusting sub-module;
the light source intensity adjusting submodule is used for adjusting and controlling the light intensity of the fluorescent light source (1);
the pH value adjusting submodule is used for adjusting the pH value of the to-be-detected liquid (9) of the fluorescent probe;
the fluorescent probe concentration adjusting submodule is used for adjusting the volume of the fluorescent probe to-be-detected liquid (9) and the probe concentration;
and the temperature adjusting submodule is used for adjusting the temperature of the liquid (9) to be detected of the fluorescent probe.
8. The apparatus for automated detection of the environmental parameter response characteristics of fluorescent probes according to claim 1, wherein said environmental parameter regulation module comprises a stirring submodule comprising a magnetic stirrer (29) connected to a central controller (4).
9. The apparatus of claim 2, wherein the fluorescent light source module, the fluorescent signal detection module, the environmental parameter sensing module, and the environmental parameter control module are disposed inside a device housing capable of isolating environmental light.
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