CN110643589A - 一种提高植物抗旱性的蛋白及其应用 - Google Patents

一种提高植物抗旱性的蛋白及其应用 Download PDF

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CN110643589A
CN110643589A CN201910884632.1A CN201910884632A CN110643589A CN 110643589 A CN110643589 A CN 110643589A CN 201910884632 A CN201910884632 A CN 201910884632A CN 110643589 A CN110643589 A CN 110643589A
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代明球
张卉
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Huazhong Agricultural University
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Abstract

本发明公开了一种提高植物抗旱性的蛋白及其应用，属于植物遗传育种领域。本发明提供的蛋白氨基酸序列如SEQ ID NO.1‑SEQ ID NO.3所示，或氨基酸序列具有与SEQ ID NO.1所示序列至少70％相似度的蛋白。进一步地，本发明公开了编码上述蛋白的核酸，以及包含该核酸的表达盒、表达载体、宿主细胞及其在提高植物抗旱性中的应用。

Description

一种提高植物抗旱性的蛋白及其应用
技术领域
本发明涉及一种提高植物抗旱性的蛋白及其应用，属于植物遗传育种技术领域。
背景技术
植物在其生长和发育过程中不可避免地要遭受各种环境胁迫的影响或伤害，长期地进化使植物演化出适应或对付各种胁迫的机制或策略。细胞信息传递是植物实现抗逆的根本基础。植物的细胞逆境信息传递是非常复杂的，其中植物激素脱落酸(abscisic acid，ABA)是最为关健的信号组分之一。有很多调控因子介导着ABA信号的调控，其中蛋白磷酸酶是重要的ABA信号受体。
蛋白质磷酸化是调节和控制蛋白质活力和功能的最基本、最普遍，也是最重要的机制。蛋白磷酸化受到蛋白激酶和磷酸酶的调控。蛋白磷酸酶是具有催化已经磷酸化的蛋白质分子发生去磷酸化反应的一类酶分子(Luan S.Protein phosphatases inplants[J].Annu Rev Plant Biol,2003,54:63-92.)。已有研究表明，部分蛋白磷酸酶(如丝氨酸/苏氨酸蛋白磷酸酶、酪氨酸蛋白磷酸酶)会影响ABA信号的积累、传导，并与ABA信号的其他受体发生互作(张继红，陶能国.植物PP2C蛋白磷酸酶ABA信号转导及逆境胁迫调控机制研究进展[J].广西植物，2015(6)：935-941；石武良，贾文锁，刘新，等.酪氨酸蛋白磷酸酶参与保卫细胞中ABA诱导产生H2O2的信号传递[J].科学通报，2004，49(16)：1617-1622.)。
干旱是影响植物正常生长的重要因素。在磷酸酶家族中挖掘具有抗旱作用的新基因并通过生物工程的手段予以利用是提高植物抗旱性的有效途径。
发明内容
本发明通过搜集拟南芥磷酸酶基因突变体，并进行抗旱表型鉴定，发现拟南芥AtPTPP基因具有抗旱的功能。进一步通过酶活性测定确定了具有活性的最小区段。因此，利用具有该区段的蛋白和编码基因能够提高双子叶植物拟南芥、单子叶植物玉米等植物的抗旱性。
本发明提供了一种可以用来提高植物抗旱性的蛋白，其特征在于，所述蛋白的氨基酸序列如如SEQ ID NO.1-SEQ ID NO.3所示；或者，所述蛋白的氨基酸序列具有与SEQ IDNO.1所示序列至少70％序列相似度的蛋白；在一些实施方案中，所述蛋白的氨基酸序列如SEQ ID NO.6至SEQ ID NO.67中的任意一个所示。
在又一方面，本发明还提供了一种核酸，其特征在于，上述核酸编码上述的蛋白；在一些实施方案中，所述核酸的序列如SEQ ID NO.4和SEQ ID NO.5所示。
在又一方面，本发明还提供了基因表达盒，其特征在于，含有权利要求3所述的核酸序列；在一些实施方案中，所述核酸与ubi启动子和nos终止子可操作地连接。
在又一方面，本发明还提供了表达载体，其特征在于，所述载体含有上述表达盒；在一些实施方案中，所述载体还包含ubiquitin:bar:CaMV35SpolyA表达盒在又一方面，本发明还提供了宿主细胞，其特征在于，包含上述表达载体；在一些实施方案中，所述宿主细胞为植物细胞或原核生物细胞；在一些实施方案中，所述宿主细胞为大肠杆菌或农杆菌细胞。
在又一方面，本发明还提供了产生转基因植物的方法，其特征在于，用上述表达载体或上述宿主细胞转化作物，得到转基因植物。
在又一方面，本发明还提供了产生转基因种子的方法，其特征在于，从上述方法产生的转基因植物产生转基因种子。
在又一方面，本发明还提供了上述蛋白、核酸、基因表达盒、表达载体、宿主细胞、方法在提高植物抗旱性上的应用；在一些实施方案中，所述植物为单子叶植物和双子叶植物；在一些实施方案中，所述植物为拟南芥和玉米。
在之前并没有公开的资料显示本发明提供的蛋白序列与增强植物抗旱性有关，因此本发明为提高植物抗旱性提供了新的途径。
附图说明
附图1SALK_023939突变体插入位置及验证。A：T-DNA插入位置和基因结构，UTR：非翻译区；Exon：外显子；Intron：内含子；B：突变体基因型检测；C：突变体Atptpp基因表达检测。
附图2AtPTPP基因编辑材料的创制和表型鉴定。A：AtPTPP基因结构、编辑靶点(gRNA)位置以及编辑后的基因结构；B：基因编辑产物的PCR检测；C：突变体(Atptpp)和基因编辑(Crispr-AtPTPP)植株与野生型植株(Col)的抗旱性比较；D：干旱条件下突变体(Atptpp)、基因编辑(Crispr-AtPTPP)与野生型(Col)植株的存活率统计数据。
附图3AtPTPP基因超表达材料的创制和表型鉴定。A：超表达(OX-5、OX-6)植株与野生型植株(Col)的抗旱性比较；B：超表达(OX-5、OX-6)与野生型(Col)植株中AtPTPP基因的相对表达量；C：干旱条件下超表达(OX-5、OX-6)与野生型(Col)植株的存活率统计数据。
附图4PTPP蛋白结构域分析。A：PTPPa、PTPPb和PTPPc结构域的序列相似性；B：PTPPa、PTPPb和PTPPc结构域在AtPTPP蛋白上的位置分布。
附图5PTPP蛋白表达的结果。
附图6PTPP蛋白的底物筛选。A：AtPTPP的植酸酶活性检测；B：AtPTPP蛋白底物筛选；C：ZmPTPP蛋白底物筛选；方框标识有反应的底物。
附图7PTPP分段蛋白活性测定。A：玉米超表达植株的抗旱性表现照片；B：存活率统计数据；(+)阳性植株；(-)阴性对照植株；35、48表示不同的转化事件。
附图8PTPP反应条件检测。A：AtPTPP；B：ZmPTPP。
附图9pZZ0153-ZmPTPP超表达载体图。各元件英文及各缩写含义列举如下：
RB T-DNA区右边界序列
Ubi promoter 玉米泛素基因启动子
ZmPTPP ZmPTPP基因
Nos 胭脂碱合成酶基因终止子
Ubi promoter 玉米泛素基因启动子
BlpR 耐除草剂草铵膦bar基因
CaMV35S ployA 花椰菜花叶病毒35SpolyA终止子
LB T-DNA区左边界序列
kanR 卡那霉素抗性序列
ori pBR322起始区
bom pBR322骨架区
pVS1 rep pVS1复制子
pVS1 sta pVS1转录起始区
附图10玉米超表达植株的抗旱表现。A：玉米超表达植株的抗旱性表现照片；B：存活率统计数据；(+)阳性植株；(-)阴性对照植株；35、48表示不同的转化事件。
附图11玉米RNAi植株的抗旱表现。A：玉米RNAi植株的抗旱性表现照片；B：存活率统计数据；(+)阳性植株；(-)阴性对照植株；85、108表示不同的转化事件。
具体实施方式
提供以下定义和方法用以更好地界定本申请以及在本申请实践中指导本领域普通技术人员。除非另作说明，术语按照相关领域普通技术人员的常规用法理解。本文所引用的所有专利文献、学术论文、行业标准及其他公开出版物等，其中的全部内容整体并入本文作为参考。
如本文所用，“玉米”是任何玉米植物并包括可以与玉米育种的所有植物品种，包括整株植物、植物细胞、植物器官、植物原生质体、植物可以从中再生的植物细胞组织培养物、植物愈伤组织、植物或植物部分中完整的植物细胞，所述植物部分例如胚、花粉、胚珠、种子、叶、花、枝、果实、茎杆、根、根尖、花药等。除非另有所指，核酸以5’至3’方向从左向右书写；氨基酸序列以氨基至羧基方向从左向右书写。氨基酸在本文可以用其通常所知的三字母符号或IUPAC-IUB生物化学命名委员会推荐的单字母符号来表示。同样地，可以用通常接受的单字母码表示核苷酸。数字范围包括限定该范围的数字。如本文所用，“核酸”包括涉及单链或双链形式的脱氧核糖核苷酸或核糖核苷酸多聚物，并且除非另有限制，包括具有天然核苷酸基本性质的已知类似物(例如，肽核酸)，所述类似物以与天然存在的核苷酸类似的方式与单链核酸杂交。如本文所用，术语“编码”或“所编码的”用于特定核酸的上下文时，指该核酸包含指导该核苷酸序列翻译成特定蛋白的必需信息。使用密码子表示编码蛋白的信息。如本文所用，涉及特定多核苷酸或其所编码的蛋白的“全长序列”指具有天然(非合成)内源序列的整个核酸序列或整个氨基酸序列。全长多核苷酸编码该特定蛋白的全长、催化活性形式。本文可互换地使用术语“多肽”、“多肽”和“蛋白”，以指氨基酸残基的多聚物。该术语用于氨基酸多聚物，其中一个或多个氨基酸残基是相应天然存在的氨基酸的人工化学类似物。该术语还用于天然存在的氨基酸多聚物。本文可互换地使用术语“残基”或“氨基酸残基”或“氨基酸”，以指被并入蛋白、多肽或肽(统称“蛋白”)的氨基酸。氨基酸可以是天然存在的氨基酸，并且除非另有限制，可以包括天然氨基酸的已知类似物，所述类似物可以与天然存在的氨基酸相似的方式起作用。
术语“性状”是指植物或特定的植物材料或细胞的生理、形态、生化或物理特性。在一些情况下，此特性对人眼是可见的，诸如种子或植株大小，或者可通过生物化学技术测量，诸如检测种子或叶的蛋白质、淀粉或油含量，或者通过观察代谢或生理过程，例如通过测量对水剥夺或特定盐或糖或氮浓度的耐受性，或者通过观察一个或多个基因的表达水平，或者通过农艺观察结果诸如渗透胁迫耐受性或收率。
“转基因”是指其基因组因异源核酸(诸如重组DNA构建体)的存在而发生改变的任何细胞、细胞系、愈伤组织、组织、植株部分或植株。本文所用的术语“转基因”包括那些最初的转基因事件以及从最初的转基因事件通过有性杂交或无性繁殖而产生的那些，并且不涵盖通过常规植物育种方法或通过自然发生的事件(诸如随机异花受精、非重组病毒感染、非重组细菌转化、非重组转座或自发突变)进行的基因组(染色体或染色体外)改变。
“植物”包括对整株植物、植物器官、植物组织、种子和植物细胞以及它们的子代的标引。植物细胞包括但不限于来自种子、悬浮培养物、胚芽、分生区域、愈伤组织、叶、根、苗、配子体、孢子体、花粉和小孢子的细胞。“子代”包含植物的任何后续世代。
在本申请中，将词语“包括”、“包含”或其变体应理解为除所描述的元素、数或步骤外，还包含其它元素、数或步骤。“受试植物”或“受试植物细胞”是指遗传改造已经生效的植物或植物细胞，或者如此改造的植物或细胞的子代细胞，该子代细胞包含所述改造。“对照”或“对照植物”或“对照植物细胞”提供用于测量受试植物或植物细胞表型改变的参考点。
阴性或对照植物可以包括，例如：(a)野生型植物或细胞，即与遗传改造起始材料具有相同基因型的植物或细胞，所述遗传改造产生受试植物或细胞；(b)与所述起始材料具有相同基因型但已用空构建体(即用对目的性状无已知效果的构建体，诸如包含标物基因的构建体)转化的植物或植物细胞；(c)是受试植物或植物细胞的非转化分离子的植物或植物细胞；(d)与所述受试植物或植物细胞在遗传上一致但未暴露于会诱导目的基因表达的条件或刺激物的植物或植物细胞；或(e)受试植物或植物细胞自身，其处于目的基因不被表达的条件下。
本领域技术人员会容易地认同，诸如位点特异性诱变和随机诱变、聚合酶链式反应方法和蛋白工程化技术的分子生物学领域的进步提供了广泛的适当的工具和操作步骤，以用于改造或者工程化农业上感兴趣的蛋白的氨基酸序列和潜在的基因序列。
在一些实施方案中，可以对本申请的核苷酸序列进行改变，以进行保守氨基酸替换。保守氨基酸替换的原则和实例在下文中进一步描述。在某些实施方案中，可以依照公开的单子叶密码子偏好性对本申请的核苷酸序列进行不改变氨基酸序列的替换，例如可以用单子叶植物偏好的密码子替换编码同一氨基酸序列的密码子，而不改变该核苷酸序列所编码的氨基酸序列。在一些实施方案中，以编码同一氨基酸序列的不同密码子替换本申请中的部分核苷酸序列，从而在改变核苷酸序列的同时不改变其编码的氨基酸序列。保守变体包括由于遗传密码子简并性而编码实施方案的蛋白中的一种的氨基酸序列的那些序列。在一些实施方案中，根据单子叶植物偏好密码子替换本申请中的部分核苷酸序列。本领域技术人员会认识到氨基酸添加和/或取代通常基于氨基酸侧链取代基的相对相似性，例如，所述取代基的疏水性、电荷、大小等等。具有各种前述所考虑性质的示例性氨基酸取代基团为本领域技术人员所公知，并且包括精氨酸与赖氨酸；谷氨酸和天门冬氨酸；丝氨酸和苏氨酸；谷氨酰胺和天冬酰胺；以及缬氨酸、亮氨酸和异亮氨酸。关于不影响目的蛋白生物学活性的适当氨基酸取代的指南可以在Dayhoff等人(1978)Atlas ofProtein Sequence andStructure(蛋白序列和结构图集)(Natl.Biomed.Res.Found.,Washington,D.C)(通过引用并入本文)的模型中找到。可以进行诸如将一个氨基酸换作具有相似性质的另一个氨基酸的保守性取代。序列一致性的鉴定包括杂交技术。例如，将已知核苷酸序列的全部或部分用作与其它相应核苷酸序列选择性杂交的探针，所述其它相应核苷酸序列存在于来自所选生物体的已克隆基因组DNA片段或cDNA片段群(即基因组文库或cDNA文库)。所述杂交探针可以是基因组DNA片段、cDNA片段、RNA片段或其它寡核苷酸，并且可以用诸如32P的可检测基团或其它可检测标志物来标记。因而，例如，可以通过标记基于实施方案序列的合成寡核苷酸制备杂交探针。制备杂交探针和构建cDNA及基因组文库的方法通常为本领域已知。可以在严紧条件下进行所述序列的杂交。如本文所用，术语“严紧条件”或“严紧杂交条件”表示如下条件，即在该条件下，相对于与其它序列杂交，探针将以可检测的更大程度(例如，背景的至少2倍、5倍或10倍)与其靶序列杂交。严紧条件是序列依赖性的并且在不同环境中有所不同。通过控制杂交严紧性和/或控制清洗条件，可以鉴定与所述探针100％互补的靶序列(同源探针法)。可选择地，可以调节严紧条件，以允许一些序列错配，以便检测较低的相似度(异源探针法)。通常，探针长度少于约1000或500个核苷酸。通常，严紧条件是如下的条件，即在该条件中，盐浓度为pH 7.0至8.3下，少于约1.5M Na离子，通常约0.01M至1.0M Na离子浓度(或其它盐)，并且温度条件为：当用于短探针时(例如10到50个核苷酸)，至少约30℃；当用于长探针时(例如大于50个核苷酸)，至少约60℃。还可以通过添加诸如甲酰胺的去稳定剂来实现严紧条件。示例性的低严紧条件包括37℃下使用30％至35％的甲酰胺缓冲液、1M NaCl、1％SDS(十二烷基硫酸钠)杂交，50℃至55℃下在1×至2×SSC(20×SSC＝3.0MNaCl/0.3M柠檬酸三钠)中清洗。示例性的中度严紧条件包括37℃下在40％至45％甲酰胺、1.0M NaCl、1％SDS中杂交，55℃至60℃下在0.5×至1×SSC中清洗。示例性的高严紧条件包括37℃下在50％甲酰胺、1M NaCl、1％SDS中杂交，60℃至65℃下在0.1×SSC中最后清洗至少约20分钟。任选地，清洗缓冲液可以包含约0.1％至约1％SDS。杂交持续时间通常少于约24小时，通常为约4小时至约12小时。特异性通常依赖杂交后的清洗，关键因素在于最后清洗溶液的离子强度和温度。DNA-DNA杂合体的Tm(热力学熔点)可以近似自Meinkoth andWahl(1984)Anal.Biochem.138:267-284的公式：Tm＝81.5℃+16.6(logM)+0.41(％GC)-0.61(％甲酰胺)-500/L；其中M是一价阳离子的克分子浓度，％GC是DNA中鸟苷和胞嘧啶核苷酸的百分数，“甲酰胺％”是杂交溶液的甲酰胺百分数，而L是杂合体的碱基对长度。Tm是(确定的离子强度和pH下)50％的互补靶序列与完全匹配的探针杂交时的温度。通常将清洗至少进行至达到平衡，并且达到低的杂交背景水平，诸如进行2小时、1小时或30分钟。每1％的错配对应使Tm降低约1℃；因而，可以调节Tm、杂交和/或清洗条件，从而与所需一致性的序列杂交。例如，如果需要≥90％一致性的序列，可以将Tm降低10℃。通常，将严紧条件选择为比确定离子强度和pH下的特异序列及其互补序列的Tm低约5℃。然而，在非常严紧的条件下，可以在比所述Tm低4℃下进行杂交和/或清洗；在中度严紧条件下，可以在比所述Tm低6℃下进行杂交和/或清洗；在低严紧条件下，可以在比所述Tm低11℃下进行杂交和/或清洗。
在一些实施方案中，还包括核苷酸序列及其编码的氨基酸序列的片段。如本文所用，术语“片段”指实施方案的多核苷酸的核苷酸序列的一部分或者多肽的氨基酸序列的一部分。核苷酸序列的片段可以编码蛋白片段，所述蛋白片段保留天然或相应全长蛋白的生物学活性，并因而具有蛋白活性。突变体蛋白包括天然蛋白的生物活性片段，其包含保留天然蛋白生物学活性的连续氨基酸残基。一些实施方案还包括转化的植物细胞或转基因植物，其包含至少一种实施方案的核苷酸序列。在一些实施方案中，使用表达载体转化植物，所述表达载体包含至少一种实施方案的核苷酸序列以及与其可操作地连接的在植物细胞中驱动表达的启动子。转化的植物细胞和转基因植物表示基因组内包含异源多核苷酸的植物细胞或植物。一般来说，所述异源多核苷酸在转化的植物细胞或转基因植物的基因组内稳定地整合，以致将所述多核苷酸传递给后代。可以将所述异源多核苷酸单独地或作为表达载体的一部分整合进基因组。在一些实施方案中，本申请涉及的植物包括植物细胞、植物原生质体、可以再生出植物的植物细胞组织培养物、植物愈伤组织、植物团块和植物细胞，其为完整的植物或者植物的部分，诸如胚胎，花粉，胚珠，种子，叶，花，枝，果实，果仁，穗，穗轴，壳，秸秆，根，根尖，花药等等。本申请还包括源于本申请的转基因植物或其子代、并因而至少部分地包含本申请的核苷酸序列的植物细胞、原生质体、组织、愈伤组织、胚胎以及花、茎、果实、叶以及根。
在一些实施方案中，本发明对拟南芥蛋白磷酸酶家族基因突变体进行了抗旱性的鉴定，发现AtPTPP基因具有提高植物抗旱性的功能。酶活性测试发现PTPPa结构域蛋白具有核酸酶活性。通过PTPPa结构域序列可以获得与PTPPa高同源性(>70％)的蛋白序列。因此，PTPPa结构域蛋白及同源蛋白，以及包含该结构域的蛋白(AtPTPP或ZmPTPP)具有提高植物抗旱性的功能。
进一步地，本发明公开了一种提高植物抗旱性的方法，即通过基因工程技术在植株内过量表达上述蛋白序列，可得到抗旱性显著提高的转基因植株。
以下实施例用于说明本发明，但不用来限制本发明的范围。在不背离本发明精神和实质的情况下，对本发明方法、步骤或条件所作的修改或替换，均属于本申请的范围。若无特别指明，实施例按照常规实验条件，如Sambrook等人的分子克隆实验手册(SambrookJ&Russell DW,Molecular cloning:a laboratory manual,2001)，或按照制造厂商说明书建议的条件。若未特别指明，实施例中所用的化学试剂均为常规市售试剂，实施例中所用的技术手段为本领域技术人员所熟知的常规手段。
实施例1拟南芥突变体的抗旱表型鉴定
本发明从拟南芥突变体库中搜集到一系列蛋白磷酸酶家族基因突变体(具体突变体信息见表1)，并对这些突变体的抗旱性状进行鉴定。鉴定的方法如下：
种子用消毒液(10％NaClO+0.025％Triton x-100)消毒10min，之后用无菌水漂洗3次去除消毒液，最后保留1mL无菌水在管中。将洗好的种子在4℃冰箱中黑暗春化3天。春化过后的种子，平铺于MS培养皿上发芽，并放在生长箱(22℃、16小时光照/8小时黑暗)生长8天左右，之后将幼苗移栽到土中(国产园艺土：进口土：蛭石＝1：1：1)。保持每个小盆中材料数目一致，土量一致。移栽之后的材料在生长室(22-24℃、16小时光照/8小时黑暗)中正常生长到3周，之后停止浇水，开始干旱处理，期间观察材料的表型情况，大概处理两周左右复水，复水后3天统计存活率。
表1拟南芥蛋白磷酸酶家族基因突变体信息
其中SALK_023939相比其他的突变体植株具有更强的抗旱性。根据突变体信息，SALK_023939的T-DNA序列插入拟南芥AtPTPP基因外显子区域。根据NCBI提供的T-DNA插入位点设计引物，对突变体进行基因型鉴定，并进行目的基因表达量分析，确认该突变体为目的基因的功能缺失突变体。结果见附图1。
基因型鉴定的引物为：
PTPP(geno)-F：CCTGGTATGCTTCTGTTTGC；
PTPP(geno)-R：TTTCATGGGTCAGAACGTCA；
LB1：ATTTTGCCGATTTCGGAAC。
表达量鉴定的引物为：
Actin3-F：GTCGTACTACCGGTATTGTGCT；
Actin3-R：CTCCAATCCAGACAGAGTATTTC。
实施例2AtPTPP基因抗旱功能验证
利用CRISPR基因编辑和超表达载体创制基因敲除和超表达材料，通过对基因编辑材料和超表达材料的抗旱表型鉴定确定基因的抗旱功能。
1、CRISPR基因编辑材料获得和表型鉴定。
首先利用CRISPR PLANT网站(http://www.genome.arizona.edu/crispr/CRISPRsearch.html)在目的基因第一个外显子区域处(附图2A)上设计两个靶位点——
gRNA1：GATACCGAAGGAGCCAGAGC；
gRNA2：CAGATCGAAGGCGCTCCTAA。
设计引物用于扩增带有目的基因gRNA的gRNA骨架。引物序列为：
DT1-BsF：ATATATGGTCTCGATTGATACCGAAGGAGCCAGAGCGTT
DT1-F0：TGATACCGAAGGAGCCAGAGCGTTTTAGAGCTAGAAATAGCDT2-R0：
AACCAGATCGAAGGCGCTCCTAACAATCTCTTAGTCGACTCTAC
DT2-BsR：ATTATTGGTCTCGAAACCAGATCGAAGGCGCTCCTAAC
以稀释100倍的pCBC-DT1T2为模板进行四引物PCR扩增。-BsF/-BsR为正常引物浓度；-F0/-R0稀释20倍。
PCR扩增体系：
模板(cDNA)：1μL；
引物(4种)：各0.5μL；
Phanta：0.4μL；
10mM dNTPmix：0.4μL；
5×SF Buffer：4μL；
ddH2O：总体积20μL；
反应程序为：
Figure BDA0002206921300000131
纯化回收PCR产物，建立如下酶切-连接体系(restriction-ligation)：
Figure BDA0002206921300000132
Figure BDA0002206921300000141
取10μL产物转化到大肠杆菌感受态XLS中(热击法)，筛选阳性菌株，提取质粒保存，完成载体构建。将构建好的质粒转到农杆菌GV3101(电击转化)中。筛选阳性菌株，用于侵染Col材料。农杆菌侵染方法：将盛花期的Col材料减去已有的果荚，留下未开的花苞。之后将28℃，200rpm过夜培养的农杆菌(200mL)，5,000g离心7min，去上清，收集菌体。之后以200mL重悬液(2.2g MS+20g蔗糖/L，PH＝5.7)重悬菌体。在侵染之前在菌液中加入50μL L77混匀。将Col花序浸泡在菌液中2min之后取出，黑暗培养24小时后，取出正常生长，直到收种。
将获得的种子消毒春化，平铺于带有1/2000(V:V)潮霉素的MS皿上进行筛选。将具有潮霉素抗性的阳性植株移栽到土中继续生长，之后提取阳性植株的DNA进行基因型鉴定。在两个gRNA两端设计引物进行PCR扩增，寻找存在大片段缺失的单株(条带明显变小)。将PCR产物送测序，来确定编辑效果。获得阳性植株后回交Col，清除CAS9(基因型鉴定存在编辑，但无潮霉素抗性)。对靶标编辑位点的检测结果显示，AtPTPP基因在第1个外显子上存在101bp的序列缺失，基因成功得到编辑(附图2B)。
对基因编辑材料的表型鉴定(方法参照实施例1)，结果显示编辑植株在干旱条件下存活率显著下降(附图2C、D)。
2、超表达材料的获得和表型鉴定
以拟南Col cDNA为模板，AtPTPP-F’/AtPTPP-R’为引物扩增AtPTPP全长CDS。以KpnI和XbaI线性化pCAMBIA1305-3HA载体。电泳跑胶回收片段和载体后，通过同源重组将片段连到载体上。构建完成pCAMBIA1305-AtPTPP-3HA超表达载体。全长扩增引物如下：
AtPTPP-F’：
ACGGGGGACGAGCTCGGTACCATGTCGATACCGAAGGAGCCAG
AtPTPP-R’：TGCCTGCAGGTCGACTCTAGATTTATCTATCCTGAGGTTATG
同源重组的体系及步骤为：
片段：碱基数×0.04ng；
载体：碱基数×0.02ng；
ExnaseII：2μL；
5×ExnaseII buffer：4μL；
ddH2O：加到总体积20μL；
37℃30min，冰浴5min
将重组产物取10μL用于转化(热击转化)大肠杆菌感受态XLS。挑选阳性克隆，扩繁提取质粒。将构建好的质粒转到农杆菌GV3101(电击转化)中，筛选阳性菌株，用于侵染Col材料。将盛花期的Col材料减去已有的果荚，留下未开的花苞。之后将28℃、200rpm过夜培养的农杆菌200mL，5,000g离心7min，去上清，收集菌体。之后以200ml重悬液(2.2g MS+20g蔗糖/L，PH＝5.7)重悬菌体。在侵染之前在菌液中加入50μL L77混匀。将Col花序浸泡在菌液中2min后取出，黑暗培养24小时，取出正常生长直到收种。
将获得的种子消毒春化，平铺于带有1/2000(V:V)潮霉素的MS皿上进行筛选。将具有潮霉素抗性的阳性植株移栽到土中继续生长，之后提取阳性植株的RNA进行AtPTPP基因的表达量分析检测。筛选获得表达量上调的阳性植株，单株收种获得T1代。之后将T1代种子继续平铺于带有1/2000(V:V)潮霉素的MS皿上进行筛选，此时材料会出现分离，将活着的阳性植株种下去继续繁种。单株收种获得T2代。之后将T2代种子继续之前的抗性筛选，这一代会出现后代全为阳性不分离的材料及纯合家系。将这种材料再种植下去，收种T3代，用于抗旱鉴定。
抗旱鉴定时，种子以消毒液(10％NaClO+0.025％Triton x-100)消毒10min，之后用无菌水漂洗3次去除消毒液，最后保留1mL无菌水在管中。将洗好的种子在4℃冰箱黑暗春化3天。春化过后的种子，平铺于MS培养皿上发苗，将培养皿放在生长箱(22℃，16小时光照/8小时黑暗)生长8天左右，之后将幼苗移栽到土中。移栽时将要比较的材料对半种植。移栽之后的材料在生长室(22-24℃，16小时光照/8小时黑暗)中正常生长到3周，之后停止浇水，大概干旱处理2周左右(具体复水时间要观察材料干的程度来定)，复水3天之后统计材料的存活率。结果如附图3所示。
实施例3PTPP酶活性测试
1、PTPP蛋白结构域分析
对AtPTPP基因编码蛋白的结构域分析发现，AtPTPP蛋白包含3个结构域PTPPa、PTPPb和PTPPc，这三个结构域的序列相似性极高(附图4)。
2、蛋白表达载体的构建。
以拟南芥Col(哥伦比亚，野生型)cDNA为模板，AtPTPP-F/AtPTPP-R，PTPPa-F/PTPPa-R，PTPPa-F/PTPPb-R，PTPPb-F/PTPPc-R，PTPPc-F/PTPPc-R为引物，分别PCR扩增，获得AtPTPP，PTPPa，PTPPab，PTPPbc，PTPPc片段。同时以玉米B73 cDNA为模板，ZmPTPP-F/ZmPTPP-R为引物扩增ZmPTPP片段。
PCR扩增体系：
Figure BDA0002206921300000161
反应程序为：
Figure BDA0002206921300000162
Figure BDA0002206921300000171
所用引物序列如下：
AtPTPP-F：
TTTCAGGGGCCCCTGGGATCCATGTCGATACCGAAGGAGCC；
AtPTPP-R：
CTTGGTACCGCATGCCTCGAGTTATTTATCTATCCTGAGGTTATG；
ZmPTPP-F：
ATCGGGCGCGGATCCCATATGATGGACGCCGGGGAGATGGAG；
ZmPTPP-R：
ATGGTGATGGTGATGCTCGAGTTTGTCCAGCTTTAGATTGT；
PTPPa-F：
CCATGGGGTACCGGATCCCATATGATGATGAGAGATGGATCGGTACTTG；
PTPPa-R：
CTCGAG TTAGTCGACAGAAGGCTTCATAGCTGCAT；
PTPPb-F：
CCATGGGGTACCGGATCCCATATGATGGCAGAATCTACCGACGGGCGC C；
PTPPb-R：GTCGACTTATTGTGATTCATGTTGTGCCC；
PTPPc-F：
CCATGGGGTACCGGATCCCATATGATGAACGAACGTAGTGGTTCTGTCT；
PTPPc-R：
CTCGAGTTAGTCGACTTTATCTATCCTGAGGTTAT。
将PCR扩增获得的AtPTPP、PTPPa、PTPPab、PTPPbc、PTPPc和ZmPTPP片段电泳跑胶，回收。分别用BamHI和XhoI，NdeI和XhoI线性化pFastBac1-Sumo和pFastBac1-Chis载体。然后将AtPTPP片段和ZmPTPP片段分别同源重组到线性化的pFastBac1-Sumo和pFastBac1-Chis载体上。构建完成蛋白表达载体pFastBac1-Sumo-AtPTPP和pFastBac1-ZmPTPP-chis。
酶切体系：
质粒：1μg；
NdeI+XhoI或NdeI+SalI：0.5μL+0.5μL；
10×buffer：2μL；
ddH2O：to 20μL；
37℃酶切3-5小时。
同源重组体系及步骤：
片段：碱基数×0.04ng
载体：碱基数×0.02ng
ExnaseII TM：2μL
5×ExnasII TM buffer：4μL
ddH2O：总体系20μL
37℃30min；冰浴5min。
进一步将PTPPa，PTPPab，PTPPbc，PTPPc连接到pJET1.2载体上。将所有载体进行测序确认片段。
连接反应体系及步骤：
回收片段产物50-200ng；
pJET1.20.5μL；
T4 ligase 0.5μL；
10×T4 ligase buffer 0.6μL；
ddH2O：总体积6μL；
16℃连接2小时。
用NdeI+XhoI限制性内切酶酶切pJET1.2-PTPPa，pJET1.2-PTPPc以及pFastBac1-Nhis载体；用NdeI+SalI限制性内切酶酶切pJET1.2-PTPPab，pJET1.2-PTPPbc以及pFastBac1-Nhis和pFastBac1-chis载体。之后电泳跑胶，回收PTPPa，PTPPab，PTPPbc，PTPPc片段，以及线性化的pFastBac1-Nhis和pFastBac1-chis载体。之后将PTPPa，PTPPab，PTPPc连接到pFastBac1-Nhis上，PTPPbc连接到pFastBac1-chis载体上。构建完成昆虫系统蛋白表达载体pFastBac1-Nhis-PTPPa，pFastBac1-Nhis-PTPPc，pFastBac1-Nhis-PTPPab，pFastBac1-PTPPbc–Chis。
酶切体系：
质粒：1μg；
NdeI+XhoI或NdeI+SalI：0.5μL+0.5μL；
10×buffer：2μL；
ddH2O：总体积20μL；
37℃酶切3-5小时。
连接反应体系及步骤：
载体+片段(所加的量可调整，摩尔体积比1:3，二者总体积4.9μL)
T4 ligase：0.5μL
10×T4 ligase buffer：0.6μL
16℃连接2-5小时
3、蛋白表达及纯化。
将构建好的质粒通过热击转化的方法，转入DH10Bac宿主菌中。通过DH10Bac细胞表达，扩繁获得相应的病毒杆粒，之后提取病毒杆粒，转染SF-9昆虫细胞，通过SF-9昆虫细胞表达相应蛋白。2,000g、15min离心，收集30mL昆虫细胞。以预冷的3mL lysis buffer(25mM Tris-HCl，pH 8.0，150mM NaCl，0.5mM phenylmethanesulfonyl-fluoride(PMSF))重悬细胞，在液氮中反复冻融3次破碎细胞，之后20,000g，4℃离心1小时。收集上清过Ni-NTA柱(Qiagen)，再用5mL lysis buffer(+10mM imidazole)洗柱子3次。之后用3mL洗脱液洗脱蛋白(25mM Tris-HCl pH 8.0，250mM imidazole)，蛋白测浓度备用。
实验结果见附图5。通过昆虫表达系统，成功获得了PTPPa、PTPPc、PTPPab、PTPPbc、AtPTPP(PTPP)、ZmPTPP的表达蛋白。
4、酶活测定。
对获得的重组表达蛋白进行酶活性测定。使用的基础酶活检测体系(160μL)为：
50mM HEPES-KOH buffer；
0.25mM底物；
蛋白：1μg。
做初期底物筛选时，反应体系HEPES-KOH buffer的PH＝7.5，反应温度为37℃，时间为20min。
检测酶的最适温度时，反应体系HEPES-KOH buffer的PH＝7.5，反应温度为20-70℃，时间为15min，底物为GMP和dGMP。
检测酶的最适PH时，反应体系的buffer分别为：glycine-HCl pH 2.5-4；Naacetate-acetic acid pH4.5-5.5；Tris-acetic acid pH6.0-6.5；HEPE-KOH pH7.0-8.0；反应温度为30℃，时间为15min，底物为GMP和dGMP。
检测金属离子对酶活性的影响时，反应体系HEPES-KOH buffer的PH＝7.5(加入0-10mM的Ca2+,Mg2+,Mn2+,Cu2+)，反应温度为30℃，时间为15min，底物为GMP和dGMP。反应完成后立即加入40μL孔雀石绿显色剂，混匀，放5min。之后检测反应液630nm处的吸光度值。计算反应生成的pi的量(以KH2PO4做标曲)。通过每mg蛋白，每分钟反应生成的Pi的量来反应酶活性的强弱。
进一步针对AtPTPP和ZmPTPP全长蛋白，进行底物筛选，将该蛋白一系列化合物底物进行反应，检测是否可以与这些底物反应。结果发现AtPTPP和ZmPTPP可以与GMP、GDP、dGMP、IMP、dIMP反应，因此这两个蛋白具有核苷酸酶活性。结果见附图6。
为了进一步明确蛋白的活性区域，对PTPP分段蛋白进行了活性测定，发现PTPPa、PTPPab段蛋白具有活性，PTPPb、PTPPbc段不具有活性。因此，该酶的活性位点位于PTPPa段。结果见附图7。
进一步检测了PTPP核苷酸酶的最适反应条件。结果表明该酶的最适反应条件为PH＝5.0，温度为30℃。并且适量Ca2+、Mg2+、Mn2+的加入能够提高酶的活性。结果见附图8。
实施例4PTPPa蛋白的同源序列
由于PTPPa蛋白具有核苷酸酶的活性，而且含有PTPPa序列的蛋白AtPTPP蛋白具有增强植物抗旱的功能。因此，找到包含与PTPPa蛋白序列同源的蛋白，就能够应用该蛋白序列提高植物的抗旱性。
利用BLASTP工具在NCBI数据库中(https://www.ncbi.nlm.nih.gov/)分析发现，PTPPa蛋白在其他物种中存在着序列相似度极高的蛋白，它们与PTPPa蛋白的氨基酸序列相似度至少在70％以上(表2)，氨基酸序列如SEQ ID NO.12-20所示。这说明这些蛋白与PTPPa蛋白具有潜在的功能同一性，含有这些序列的蛋白能够用来提高植物的抗旱性。
表2含有PTPPa结构域的同源蛋白
Figure BDA0002206921300000221
Figure BDA0002206921300000231
实施例5利用ZmPTPP蛋白提高玉米的抗旱性
玉米是重要的单子叶作物，提高玉米植株的抗旱性能够提高玉米产量，减少水分的施用、降低种植成本。作为拟南芥AtPTPP蛋白的同源蛋白，玉米中的ZmPTPP与AtPTPP蛋白具有极高的相似性。因此利用AtPTPP或ZmPTPP能够增强单子叶植物玉米的抗旱性。
1、玉米ZmPTPP超表达载体的构建。
以B73 cDNA为模板，ZmPTPP-F1/R1为引物扩增ZmPTPP CDS全长，所用引物序列为：
ZmPTPP-F1:
CAAACGCACTAGTATCCCGGGATGGACGCCGGGGAGATG；
ZmPTPP-R1：
GAACGATAAGCTTATGGCGCGCCCTATTTGTCCAGCTTTAGATTGTCG。
电泳跑胶，回收片段。同时酶切线性化载体pZZ0153，跑胶回收线性化载体。再通过同源重组将ZmPTPP连接到pZZ0153载体上得到pZZ0153-ZmPTPP超表达载体。ZmPTPP基因表达盒包括ubiquitin启动子和nos终止子。载体图见附图9。
载体的构建过程如下：
PCR扩增体系：
模板(cDNA)：1μL；
ZmPTPP-F1/R1：各0.5μL；
Phanta：0.4μL；
10mM dNTPmix：0.4μL；
5×SF Buffer：4μL；
ddH2O：总体积20μL。
反应程序为：
酶切线性化载体步骤：
质粒：1μg；
ASCI+SmaI：0.5+0.5μL；
10×buffer：2μL；
ddH2O：加至总体积20μL；
37℃，3-5h。
同源重组体系及步骤：
片段：碱基数×0.04ng；
载体：碱基数×0.02ng；
ExnaseII：2μL；
5×ExnaseII buffer:4μL
ddH2O：加到总体积为20μL
37℃30min；冰浴5min。
2、超表达转基因玉米植株的获得。
利用农杆菌介导方法将pZZ0153-ZmPTPP载体转入农杆菌EHA105。遗传转化的步骤如下：
⑴将载体通过电击法转入农杆菌菌株EHA105的宿主细胞中，利用PCR方法进行鉴定；
⑵以新鲜剥离的1mm左右的自交系C01玉米幼胚为材料，将剥取的玉米胚放入含有1.8mL悬浮液的2ml塑料离心管中，30min内大约处理未成熟幼胚150个；
⑶吸去悬浮液，余下玉米胚在管中然后加入1.0mL农杆菌悬浮液，放置5min。将离心管中的幼胚悬浮后倒入共培养基上，并用移液器吸去表面多余的农杆菌菌液，于23℃黑暗共培养3天；
⑷共培养后，将幼胚转移到休息培养基中，于28℃黑暗培养6天后，放至含5mg/LBialaphos的筛选培养基上，开始筛选培养两周，然后转到含8mg/L Bialaphos筛选培养基上筛选培养2周；
⑸将抗性愈伤组织转移至分化培养基1中，25℃，5000lx，光照培养1周；
⑹再将愈伤转移至分化培养基2中，光照培养2周；
⑺将分化生出的小苗转移至生根培养基上，25℃，5000lx，光照培养直到生根；
⑻将小苗转入小盆中生长，一定生长阶段后移栽于温室中，期间喷施除草剂草铵膦(商品名保试达，拜耳科学公司生产的18％草铵膦水剂)并进行PCR检测筛选得到20个阳性转基因植株，3-4个月后收获表达量上升的的T0转基因阳性种子。
3、转基因玉米的抗旱性鉴定。
将T1种子放在湿润的滤纸上发苗(室温，一般25-28摄氏度左右。)待种子冒尖之后，挑选长势一致的幼苗进行移栽，将阴性(与阳性植株同一家系分离出来的阴性植株)和阳性植株对半种植在同一白色浅盆中，每种材料种植20株。土壤为园艺土：大田土：沙＝1：1：1。之后将材料放在温室凉棚下(温度为自然温度，不定，一般在春秋季为好，温度一般在20℃～30℃的温度为好)。正常浇水，待幼苗长到4叶期，开始停止浇水，一般干旱处理时间为10-15天(具体要看天气而定)，处理过后，给材料复水。复水一周后拍照，统计材料的存活情况。
结果显示，超表达玉米植株35(+)和48(+)在干旱处理并复水后的存活率显著高于对照玉米植株35(-)和48(-)。结果见附图10。
实施例6ZmPTPP基因抗旱功能的确认
为了进一步确认ZmPTPP的抗旱功能，本发明进一步通过RNAi方式干涉玉米ZmPTPP基因的表达，并鉴定RNAi植株的抗旱性表现。
在基因的外显子上选择一段特异性比较好的序列作为RNAi的干涉片段。以B73cDNA为模板，PCR扩增RNAi片段(所用引物序列为：ZMPTPP(RNAi)-F：GGCGCGCCACTAGTGGAGGGCGACCTGTGCTATG；ZMPTPP(RNAi)-R：CCCGGG GTTTAAACAGTAACATTGAAGGCTATCGTGTC)。电泳跑胶，回收RNAi片段。将该片段连接到PJET1.2上。用SpeI和PmeI限制性内切酶将该片段从PJET1.2-RNAi上酶切下来，同时用SpeI和PmeI限制性内切酶线性化pZZ0153-PDKin载体。跑胶，回收RNAi片段和载体，用T4连接酶将RNAi片段连接到pZZ0153-PDKin上，构建完成pZZ0153-ZmPTPP(RNAi)-PDKin。之后用XmaI和AscI限制性内切酶将RNAi片段从PJET1.2-RNAi上酶切下来，同时用XmaI和AscI限制性内切酶线性化pZZ0153-ZmPTPP(RNAi)-PDKin载体。之后电泳跑胶，回收RNAi片段和载体，用T4连接酶将RNAi片段连接到RNAi表达载体上，载体的转化和抗旱鉴定方法参照实施例5。
抗旱鉴定结果显示，RNAi玉米植株85(+)和108(+)在干旱处理并复水后的存活率显著低于对照玉米植株85(-)和108(-)。结果见附图11。这进一步证明了ZmPTPP基因及其编码蛋白在植物中的抗旱作用。
虽然，上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。
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<120> 一种提高植物抗旱性的蛋白及其应用
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565 570 575
Gly Gln Ile Phe Asp Leu Trp Glu Asn Val Asp Ala Asp Ser Val Gln
580 585 590
Thr Pro Leu Glu Val Tyr Lys Cys Leu Glu Ala Asp Gly Phe Pro Ile
595 600 605
Lys Tyr Ala Arg Val Pro Ile Thr Asp Gly Lys Ala Pro Lys Ser Ser
610 615 620
Asp Phe Asp Thr Leu Thr Ser Asn Ile Ala Ser Ala Ser Lys Asp Thr
625 630 635 640
Ala Phe Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly
645 650 655
Thr Val Ile Ala Cys Leu Val Lys Leu Arg Ile Asn Tyr Gly Arg Pro
660 665 670
Ile Lys Val Leu Tyr Asp Val Leu Thr His Glu Ile Val Asp Glu Asp
675 680 685
Ser Ser Ser Gly Gly Glu Glu Thr Gly Ser Asn Asn Ala Glu Ala Arg
690 695 700
Pro Arg Asn Ser Gly Arg Arg Thr Glu Glu Glu Gln Gly Arg Ala Phe
705 710 715 720
Gly Met Asp Asp Ile Leu Leu Leu Trp Lys Ile Thr Arg Leu Phe Asp
725 730 735
Asn Gly Val Glu Ser Arg Glu Ala Leu Asp Ala Val Ile Asp Arg Cys
740 745 750
Ser Ala Leu Gln Asn Ile Arg Glu Ala Val Leu Gln Tyr Arg Lys Val
755 760 765
Phe Asn Gln Gln His Val Glu Pro Arg Val Arg Ser Ala Ala Leu Lys
770 775 780
Arg Gly Ala Glu Tyr Leu Glu Arg Tyr Phe Arg Leu Ile Ala Phe Ala
785 790 795 800
Ala Tyr Leu Gly Ser Lys Ala Phe Asp Gly Phe Phe Val Glu Gly Glu
805 810 815
Ser Lys Val Thr Phe Lys Asn Trp Leu His Gln Arg Pro Glu Val Gln
820 825 830
Ala Met Lys Trp Ser Ile Arg Leu Arg Pro Gly Arg Phe Phe Thr Ile
835 840 845
Pro Glu Glu Leu Arg Ala Gln His Glu Ser Gln His Gly Asp Ala Val
850 855 860
Met Glu Ser Ile Val Asn Glu Arg Ser Gly Ser Val Leu Ser Lys Gly
865 870 875 880
Ser Ile Leu Lys Met Tyr Phe Phe Pro Gly Gln Arg Thr Ser Ser Arg
885 890 895
Leu Gln Ile Asn Gly Ala Pro His Val Tyr Lys Val Asp Arg Tyr Pro
900 905 910
Val Tyr Ser Met Ala Thr Pro Thr Ile Ser Gly Ala Lys Lys Met Leu
915 920 925
Ala Tyr Leu Gly Thr Lys Leu Lys Glu Glu Gly Gly Gly Ser Thr Glu
930 935 940
Arg Ile Val Val Thr Asp Leu Arg Glu Glu Ala Val Val Tyr Ile Asn
945 950 955 960
Gly Thr Pro Phe Val Leu Arg Glu Leu Ser Lys Pro Val Asp Thr Leu
965 970 975
Lys His Val Gly Ile Thr Gly Ala Val Val Glu Ser Leu Glu Thr Arg
980 985 990
Leu Lys Glu Asp Ile Leu Ala Glu Val Arg Glu Thr Gly Gly Arg Met
995 1000 1005
Leu Leu His Arg Glu Glu Tyr Ser Pro Ala Ser Asn Gln Ser Arg Val
1010 1015 1020
Ile Gly Tyr Trp Glu Asn Ile Gln Pro Glu Asn Val Lys Thr Pro Ala
1025 1030 1035 1040
Glu Val Tyr Ala Ala Leu Lys Asp Glu Asn Tyr Asn Ile Ser Tyr Arg
1045 1050 1055
Arg Ile Pro Leu Thr Arg Glu Lys Asp Ala Leu Ala Ser Asp Val Asp
1060 1065 1070
Ala Ile Gln Tyr Cys Lys Asp Asp Ser Ala Gly Ser Tyr Leu Phe Val
1075 1080 1085
Ser His Thr Gly Phe Gly Gly Val Ser Tyr Ala Met Ala Ile Thr Cys
1090 1095 1100
Leu Leu Leu Gln Pro Gly Gln Asn Phe Thr Ala Thr Pro Thr Thr Asn
1105 1110 1115 1120
Ser Ser Thr Leu Glu Glu Asp Asp Ser Pro Ser Gly Ala Cys Asp Glu
1125 1130 1135
Glu Ala Leu Ser Met Gly Asp Tyr Arg Asp Ile Leu Ser Leu Ile Arg
1140 1145 1150
Val Leu Ser His Gly Pro Gln Ser Lys Ser Asp Val Asp Gly Ile Val
1155 1160 1165
Glu Leu Cys Ala Gly Ala Gly His Leu Arg Glu Asp Ile Val Tyr Tyr
1170 1175 1180
Ser Lys Glu Leu Asn Lys Leu Pro Ile Thr Lys Asp Glu Asn Arg Ser
1185 1190 1195 1200
Tyr Ile Met Asp Met Gly Val Lys Ala Leu Arg Arg Tyr Phe Tyr Leu
1205 1210 1215
Ile Thr Phe Arg Ser Tyr Leu Tyr Ser Thr Ser Pro Glu Glu Met Lys
1220 1225 1230
Phe Leu Asp Trp Met Lys Ser Arg Pro Glu Leu Gly His Leu Cys His
1235 1240 1245
Asn Leu Arg Ile Asp Lys
1250
<210> 3
<211> 1264
<212> PRT
<213> 玉米(Zea mays)
<400> 3
Met Asp Ala Gly Glu Met Glu Asp Val Arg Ala Ala Ala Ala Ala Glu
1 5 10 15
Glu Val Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile
20 25 30
Leu Lys Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro
35 40 45
Gln Ile Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val
50 55 60
His Gly Val Ala Met Pro Thr Met Glu Gly Ile Ala Asn Val Leu Asn
65 70 75 80
His Ile Gly Ala His Lys Lys Gly Lys Gln Thr Arg Ile Leu Trp His
85 90 95
Ser Leu Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val
100 105 110
Leu Arg Asp Val Glu Lys Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile
115 120 125
Asn Arg Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile
130 135 140
Leu Gln Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu
145 150 155 160
Leu Pro Ser Gly Gln Met Val Asp Gln Trp Glu Ser Val Val Tyr Asp
165 170 175
Thr Val Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly
180 185 190
Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro
195 200 205
Lys Glu Gly Asp Phe Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp
210 215 220
Ile Glu Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr
225 230 235 240
Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr Leu Asn Arg Ile Gly
245 250 255
Ala Ser Gly Ile Pro Arg Thr Gly Ser Ile Gly Lys Val Phe Tyr Ala
260 265 270
Gly Asn Asp Val Asp Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu
275 280 285
Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly
290 295 300
Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp
305 310 315 320
Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Asn Ile
325 330 335
Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe
340 345 350
Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr
355 360 365
Val His Ser Val Ser Ser Ala His Gln Thr Thr Leu Ser Val Glu Val
370 375 380
Ser Phe Ser Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu
385 390 395 400
Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser
405 410 415
Ser Lys Pro Pro Leu Thr Lys Ile Val Glu Ser Ala Asn Gly Arg Pro
420 425 430
Gln Asp Met Asp Val Val Ala Ala Met Arg Asn Gly Glu Val Leu Gly
435 440 445
Arg Leu Thr Val Leu Lys Ser Asp His Cys Pro Gly Ser His Ser Leu
450 455 460
Asn Leu Pro Glu Arg Val Glu Gly Ala Pro Asn Phe Arg Glu Ile Pro
465 470 475 480
Gly Phe Pro Val Tyr Gly Val Ala Asn Pro Thr Val Asp Gly Ile Arg
485 490 495
Ala Val Ile Gln Cys Ile Ser Thr Ser Lys Gly Gly Arg Pro Val Leu
500 505 510
Trp His Asn Met Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Lys Pro
515 520 525
Tyr Val Leu Arg Glu Val Glu Arg Pro Cys Lys Asn Met Leu Glu Tyr
530 535 540
Thr Gly Ile Asp Arg Asp Arg Val Glu Arg Met Glu Ala Arg Leu Lys
545 550 555 560
Glu Asp Ile Leu Arg Glu Ala Glu Arg Tyr Gly Gly Ala Ile Met Val
565 570 575
Ile His Glu Thr Asp Asp Gly Glu Ile Phe Asp Thr Trp Glu Asn Val
580 585 590
Asp Asn Glu Ala Val Leu Thr Pro Leu Glu Val Tyr Lys Asn Leu Val
595 600 605
Ser Glu Gly Leu Pro Ile Lys Tyr Ala Arg Val Pro Ile Thr Asp Gly
610 615 620
Lys Ala Pro Lys Ser Ser Asp Phe Asp Thr Ile Ala Phe Asn Val Thr
625 630 635 640
Ala Ala Ser Lys Gly Thr Ala Phe Val Phe Asn Cys Gln Met Gly Arg
645 650 655
Gly Arg Thr Thr Thr Gly Thr Val Ile Ala Cys Leu Leu Lys Leu Arg
660 665 670
Ile Asp His Gly Arg Pro Ile Arg Ile Pro Val Cys Gln Tyr Asp His
675 680 685
Glu Asp Asp Ala Ile Tyr Ser Thr Gly Gly Asp Ala Thr Asp His Asn
690 695 700
Gly His Leu Asn Ser Glu Ser Trp Lys Pro Arg Thr Leu Thr Lys Leu
705 710 715 720
Asn Ser Gly Phe Gly Ile Asn Asp Ile Leu Leu Leu Arg Lys Ile Thr
725 730 735
Arg Leu Phe Asp Asn Gly Ile Glu Cys Arg Gln Thr Leu Asp Thr Val
740 745 750
Ile Asp Arg Cys Ser Ala Leu Gln Asn Ile Arg Gln Ala Val Leu Asn
755 760 765
Tyr Thr Arg Val Ile Asn Gln Gln His Val Glu Pro Arg Val Arg Arg
770 775 780
Val Ala Leu Asn Arg Gly Ala Glu Tyr Leu Glu Arg Tyr Leu Lys Leu
785 790 795 800
Ile Ala Phe Ser Ala Tyr Leu Gly Ser Glu Ala Phe Asp Gly Phe Cys
805 810 815
Gly Gln Gly Gln Thr Lys Ile Ser Phe Lys Gly Trp Leu Gln Gln Arg
820 825 830
Pro Glu Ile Gln Thr Met Lys Trp Ser Ile Arg Leu Arg Pro Gly Arg
835 840 845
Phe Phe Thr Val Pro His Glu Gln Lys Ala Thr Tyr Pro Pro Pro Gln
850 855 860
Gly Asp Val Thr Met Glu Thr Ile Val Lys Ala Arg Ser Gly Ser Val
865 870 875 880
Leu Gly Lys Gly Ser Ile Leu Lys Met Tyr Phe Phe Pro Gly Gln Lys
885 890 895
Arg Ser Ser Ser Val Asn Phe Arg Gly Ala Pro His Val Ile Lys Val
900 905 910
Asp Gly Tyr Pro Val Tyr Ser Met Ala Thr Pro Thr Ile Asp Gly Ala
915 920 925
Ser Asp Val Leu Ser Tyr Leu Gly Ser Lys Asp Thr Thr Gly Arg Asn
930 935 940
Ile Ala Gln Lys Val Val Val Thr Asp Leu Arg Glu Glu Val Val Val
945 950 955 960
Tyr Ile Lys Gly Ser Pro Phe Val Leu Arg Glu Leu Asp Gln Pro Val
965 970 975
Asp Thr Leu Lys His Val Gly Ile Ser Gly Pro Met Val Glu Asn Ile
980 985 990
Glu Thr Arg Leu Lys Glu Asp Ile Leu Ser Glu Val Lys Gln Leu Gly
995 1000 1005
Gly Arg Leu Leu Leu His Gln Glu Glu Phe Asn Ala Ala Thr Asn Gln
1010 1015 1020
Cys Ser Val Val Gly Tyr Trp Glu His Ile Asp Leu Glu Asp Val Met
1025 1030 1035 1040
Thr Pro Ala Glu Val Tyr Ser Thr Leu Arg Asp Lys Gly Tyr Cys Ile
1045 1050 1055
Glu Tyr Lys Arg Ile Pro Leu Thr Arg Glu Arg Glu Ala Leu Ala Ala
1060 1065 1070
Asp Val Asp Ala Ile Gln Ser Ser Ile Asp Glu Ser Ala Arg Tyr Tyr
1075 1080 1085
Leu Phe Ile Ser His Thr Gly Tyr Gly Gly Val Ala Tyr Ala Met Ala
1090 1095 1100
Ile Thr Cys Leu Gly Leu Ser Ala Asp Ala Lys Phe Val Met Glu Glu
1105 1110 1115 1120
Thr Ala Glu Thr His Phe Val Ser Thr Ser Leu Ala Lys Asn Val Ser
1125 1130 1135
Ile Lys Thr Lys Thr Asp Ile Ala Leu Lys Gln Gly Asp Tyr Arg Asp
1140 1145 1150
Ile Leu Asn Leu Thr Arg Val Leu Val His Gly Pro Lys Ser Lys Glu
1155 1160 1165
Glu Val Asp Thr Val Ile Asp Arg Cys Ser Gly Ala Gly His Leu Arg
1170 1175 1180
Glu Asp Ile Met His Tyr Arg Lys Ala Leu Gln Asp Cys Ser His Asp
1185 1190 1195 1200
Asp Asp Asp Asp Cys Asp Glu Glu His Ser Tyr Leu Thr Asp Met Gly
1205 1210 1215
Thr Lys Ala Leu Arg Arg Tyr Phe Phe Leu Ile Thr Phe Arg Ser Tyr
1220 1225 1230
Leu Tyr Ser Thr Thr Ser Cys Lys Thr Thr Phe Thr Ser Trp Met Lys
1235 1240 1245
Ala Arg Pro Glu Leu Gly His Leu Cys Asp Asn Leu Lys Leu Asp Lys
1250 1255 1260
<210> 4
<211> 3765
<212> DNA
<213> 拟南芥(Arabidopsis thaliana)
<400> 4
atgtcgatac cgaaggagcc agagcaggtg atgaagatga gagatggatc ggtacttggt 60
aagaagacga ttcttaagag tgaccatttt ccaggttgtc agaataagcg tatgactcct 120
cagatcgaag gcgctcctaa ttaccgtcag gctgattcac tacgtgtaca tggggtggca 180
attccaactg ctgtggggat acgaaacgtt cttagacaca ttggagctca caaggacggt 240
aaacaagtaa aagttctttg gattagtctc agggaggaac cggtagtata cataaacggg 300
cgaccttttg ttctacgtga tgtggagaaa cctttcacca atcttgagta cacgggaatc 360
aacagggtaa gggtagagca aatggaagca cgattaaaag aagatattct catggaggcg 420
tcgagatatg ggaataagat acttgtcacc gatgaactgc cagatggtca gatggtggac 480
cagtgggagc cagtctcaac tgattctttg aagacactac ttgaggttta cgaagaactg 540
caagctgaag gctaccttgt cgattatgaa cgtgttccta taacggatga aaagtcacca 600
aaagaaacag attttgatct gttgattagg aagatatctc aggctgacat taatacggag 660
atcattttca attgtcaaat gggacgtgga cgcacgacga ctggaatggt gattgcaacc 720
ctggtctatt tcaaacgtac tggggcatca gatcaaggat tcccaagaaa caactctttt 780
gggagaatct ttaaagctgg tgaaaatatt actgttaatc tgccgaattc ggaggaggca 840
atccgtagag gggaatatgc ggtcgttaga agtttaatta gagtcttaga gggtggtgta 900
gaaggaaaaa ggcaagttga taaagtcatc gacaaatgtg catccatgca gaatctacga 960
gaggcaattg caacctatcg cagtagtatt ttgcgtcaac ccgatgaaaa gaagagagag 1020
gcagcactct cattttttgt tgagtacttg gaaagatatt acttccttat atgctttgca 1080
gtatatcttc attcggaggg tgcttttctc caatctggtt cgcttggcca tgtcagtttt 1140
gctgattgga tgcgagctag gccagagctc tatagcattc ttcgcaggtt gctcaggaga 1200
gatcctatgg gtgcccttgg atatgcagct atgaagcctt ctttgataaa gattgcagaa 1260
tctaccgacg ggcgccctca cgagatgagt gtcgttgctg cgcttagaag tggggcagtt 1320
ctgggaagtc aaactgttct aaagagtgat cacagtcctg gctgtcaaat tttaaatcta 1380
ccagagaggg tggaaggagc tccgaatttt agggaggttc caggatttcc tgtgtatggt 1440
gttgcaaatc caactataga tggtattcga tctgtcattg aaagggttgg gagttccaga 1500
ggtggtcggc cagttttttg gcacaatatg agagaagaac ctgttatata tatcaacggg 1560
aaaccatttg ttctacgaga agttgaaagg ccatataaga acatgctaga atatacggga 1620
attgatcgag atagagttga agggatggag gctcgtctaa aagaggatat tctgagagaa 1680
gctaagcgat atgacggtgc gataatggtc attcatgaaa ccaaagatgg acagattttt 1740
gacttatggg aaaatgtaga tgctgattct gttcaaacac ccctcgaggt ttacaaatgt 1800
ttagaggctg atggttttcc tattaagtat gcacgtgtac ctataactga tggtaaagcc 1860
cccaaaagct cagactttga cacattgaca tctaatattg cttctgcttc caaagacact 1920
gcttttgtct tcaattgcca gatggggaga ggtagaacaa ccactggaac tgtcattgcc 1980
tgtctagtga agctccgcat taattatggg agacctatca aggttcttta tgacgttctg 2040
acccatgaaa ttgtggatga agattcctca agtggtggtg aagaaactgg aagtaataat 2100
gctgaagcaa gacctcgtaa ttcaggacga agaactgagg aggaacaagg tcgtgcattt 2160
ggaatggatg acatcctttt gctgtggaaa atcactaggt tattcgataa tggagttgaa 2220
tcacgtgagg cgttagatgc tgtgattgat agatgttctg cattgcaaaa tattcgtgaa 2280
gctgttctgc agtacaggaa ggttttcaac caacaacatg ttgagccacg agtaaggagt 2340
gctgccttga aacgtggcgc tgaatacttg gagagatact tccgcttgat tgcctttgca 2400
gcctatctag gaagcaaagc ttttgatgga ttctttgtgg aaggagaatc taaggtgacg 2460
ttcaagaatt ggttgcatca gaggcctgag gtgcaagcaa tgaagtggag cataaggcta 2520
agacctgggc gattttttac cattcctgag gaattgcggg cacaacatga atcacaacac 2580
ggagatgcag tcatggagtc aattgtcaac gaacgtagtg gttctgtctt gagcaaaggt 2640
tctatactta aaatgtactt tttccctggc caaagaactt caagtcgttt gcaaattaac 2700
ggtgcacctc acgtatacaa ggtcgataga tatcctgtgt atagcatggc aactcccaca 2760
atttcaggcg ctaaaaaaat gctcgcctat ttaggcacca aacttaagga agaaggtggg 2820
ggttcaacag aaagaatagt ggtaacagac ttgagagaag aagcggttgt ttacatcaat 2880
ggaactccat ttgtgttgag agaattgagt aaacctgttg atacgcttaa gcatgttgga 2940
ataaccggtg cagtggtaga aagcttagaa acacggttaa aagaagatat attggctgaa 3000
gttcgagaga ctggaggccg aatgctattg catcgtgaag aatacagccc ggcatcaaat 3060
caatctcggg ttattggata ttgggagaat attcaaccag aaaatgtgaa gacacctgca 3120
gaagtatatg ctgctctgaa agatgaaaat tacaatatat cttaccggag aataccttta 3180
accagagaaa aagacgcatt agcttccgat gtagacgcga tccagtactg taaagacgat 3240
tccgctggga gttacttgtt tgtatcgcac accggttttg gaggagtttc ttatgcaatg 3300
gctataactt gtctcctact tcaacctggt cagaacttca cagctacacc aaccacaaat 3360
tcttctacct tagaagaaga tgattcgcct tcaggggctt gtgatgaaga agcgttgagt 3420
atgggagact accgcgacat actgagtctc ataagagtgc tttctcatgg tcctcagagc 3480
aaatcagacg tagatggaat agtcgaactg tgtgctggtg caggacattt gcgagaagac 3540
atcgtttact acagtaaaga gctgaacaaa cttcctatta caaaagatga aaaccgttca 3600
tacatcatgg acatgggtgt taaagcctta aggcgctact tttacctgat aacattcaga 3660
tcatacctct atagcacgtc gccggaagaa atgaaattct tggattggat gaagtcacgg 3720
ccggaacttg gacatctctg tcataacctc aggatagata aataa 3765
<210> 5
<211> 3795
<212> DNA
<213> 玉米( Zea mays)
<400> 5
atggacgccg gggagatgga ggacgtgcgg gccgccgcgg ccgccgagga ggtcatcagc 60
agcaggggcg gctccgtgct ggggaagaag accatcctca agagcgacca cttcccgggc 120
tgccagaaca agcgcctctc cccgcagatc gacggcgcgc ccaattaccg ccaggctggg 180
tcgctgcgtg ttcatggtgt tgcaatgccc acgatggaag ggattgctaa tgtgctaaat 240
catatcggag cacacaagaa aggaaagcaa acccggattc tatggcatag tcttcgggag 300
gaaccagtta tctacatcaa tggtcgaccc tttgttttgc gggatgttga aaagcccttc 360
tcgaatcttg aatacacggg aattaaccgg gagagagtgg aacaaatgga gtttcgcctt 420
aaggaagata ttcttcagga agcttcaagg tatggtaata agatactggt cactgatgag 480
ctgccaagtg gtcagatggt ggatcagtgg gaatcagttg tgtatgatac agttaagact 540
ccacttgagg tatatgagga gttgcaacat caaggctacc tagttgacta cgaacgtgtt 600
ccaattactg atgagaaagc accaaaggaa ggagattttg acaacctggt acgtcgaatc 660
tctcaagttg atatagaaac agaaattgtg tttaactgcc aaatggggag ggggcggaca 720
actactggta tggttatagc tacattggtt tacctaaacc gaattggagc ttcaggtatc 780
cctagaacgg gctctattgg gaaggtcttc tatgctggaa atgatgtaga cgattatatg 840
cctagctcag aagaagcaat actcagaggc gaatatgctg tcattaggag tttagtccga 900
gttctcgaag gtggtgttga aggcaaaagg caagtggaca aagttattga caaatgcgac 960
tccatgcaga acctaagaga agctattgct acttaccgca ataacattct tcggcaacct 1020
gatgagatga aacgagaggc atcactttcc ttctttgtgg agtacttgga acgttattac 1080
ttccttatat gctttgctgt ctatgtgcat tcggtgagct ctgctcacca aacaacatta 1140
tcagtagaag ttagtttttc tgattggatg agagcaaggc ctgaactgta cagcattctt 1200
cgaaggctat tgagaagaga tccaatgggt gctcttggtt actcaagttc aaaaccccct 1260
cttacaaaaa ttgttgagtc tgctaatggg cgtccacaag atatggacgt tgttgctgcc 1320
atgaggaatg gagaagttct tgggcgtcta actgttttaa aaagcgacca ctgcccaggc 1380
agccacagcc ttaatttacc cgagagggtg gagggtgcac caaattttcg ggaaatccct 1440
gggtttccag tctatggtgt tgcaaatcct acagttgatg gtattcgagc tgttatccag 1500
tgcatcagta caagcaaggg agggcgacct gtgctatggc ataatatgag agaagaacca 1560
gttatctaca taaatggaaa accatatgtg ctacgcgaag tggaaagacc ttgcaagaat 1620
atgctggagt acacagggat tgatcgtgat agggtcgaac gcatggaagc tcggctgaag 1680
gaggacattt tacgcgaggc agaaagatat ggtggagcga taatggttat tcatgaaact 1740
gacgatggag agatctttga cacatgggaa aatgtagata atgaagctgt tctgactccg 1800
ttggaggtat acaaaaactt ggtgagtgaa ggccttccga tcaaatatgc acgggtgccc 1860
attactgatg gtaaagctcc caaaagttca gactttgaca cgatagcctt caatgttact 1920
gctgctagca agggcactgc tttcgtattc aattgccaga tgggcagggg cagaacaact 1980
acaggaactg tgattgcttg cttgctgaaa cttcgaattg atcatggcag acctattaga 2040
atcccagttt gtcaatatga ccatgaagat gatgctattt actcaactgg aggagatgca 2100
acagatcaca atggtcactt gaattcagaa tcatggaaac ctcgtacttt gacgaagtta 2160
aattctggat ttggaatcaa tgacattctt ctgctgcgaa aaattacaag attatttgat 2220
aatggaattg agtgtaggca gactttggat actgttattg atagatgttc agctttgcag 2280
aatattcggc aagctgtttt gaactacaca agagtaatta atcaacaaca tgttgagcct 2340
agagttagga gggttgcatt aaatcgtggc gctgaatatt tggagagata cctcaaatta 2400
attgcctttt ctgcatacct tggtagtgaa gcatttgatg gtttctgtgg acaaggacaa 2460
acaaagatct catttaaagg atggctgcaa cagcggccag agatccaaac catgaaatgg 2520
agcataagac tgcgacctgg ccgttttttc actgtgccgc atgagcagaa agcaacatat 2580
ccacctccac aaggtgatgt aacaatggaa accattgtca aagctcgtag tggttctgtt 2640
ctgggaaagg gatcaatact caaaatgtac ttctttcctg gacagaaaag gtcaagcagt 2700
gtgaatttcc ggggtgcacc tcatgttatt aaggtggatg gataccctgt gtatagcatg 2760
gcaacaccca ctattgatgg agcgagtgat gttttgtcgt atctgggctc taaagacaca 2820
accggaagaa acattgcaca gaaggtggtc gtcacagatc ttagagagga agtggttgtt 2880
tacatcaagg gatcgccatt tgttttgaga gaactagatc aacctgttga tactttgaag 2940
catgttggca ttagtggtcc aatggtagag aacatagaga caaggctaaa ggaggatata 3000
ctctctgaag ttaaacagct aggtggtcgg ttactgcttc atcaagaaga atttaatgca 3060
gccacaaatc aatgtagtgt ggtaggttac tgggaacaca tagatctgga ggacgtgatg 3120
acaccagctg aagtgtacag cacattgaga gacaagggct attgtatcga gtacaaaaga 3180
atacctctaa ccagggaaag agaagcatta gctgccgatg tagatgctat acaatcatca 3240
attgatgaat ctgctaggta ctatctcttt atttcacaca ctggatatgg aggtgttgct 3300
tatgcgatgg ccattacctg cctgggactg agtgcagatg caaagtttgt aatggaagaa 3360
acagcagaaa cacactttgt atcaacatct cttgcgaaaa atgtgtccat caaaactaaa 3420
actgacatag cacttaagca gggtgactac cgtgacatat taaaccttac tagagtgttg 3480
gtacatgggc ccaagagcaa agaagaggtt gacacagtta ttgacaggtg ttctggtgct 3540
ggtcatctgc gcgaagatat tatgcattac aggaaagcac tgcaagattg ttcacatgac 3600
gacgacgacg actgtgatga ggaacactct tatctcactg atatgggcac aaaagctttg 3660
aggcgttact tttttctgat caccttcaga tcctacctat acagcacgac atcatgcaaa 3720
acaacattta catcatggat gaaggcaagg cctgaacttg gccacctttg cgacaatcta 3780
aagctggaca aatag 3795
<210> 6
<211> 412
<212> PRT
<213> 无油樟(Amborella trichopoda)
<400> 6
Met Ser Ser Pro Lys Glu Pro Glu His Val Met Asn Tyr Arg Gly Gly
1 5 10 15
Leu Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Gln Pro His Ile Glu Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Gly Ser Leu Pro Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Asp Gly Ile Arg Asn Val Leu Asp His Ile Gly Ala Gln Lys Asp Gly
65 70 75 80
Lys Lys Thr Arg Val Leu Trp His Asn Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Ile Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asp Arg Val Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Ile Gln Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Leu Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Pro Val Ala Gln Asp Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Val Glu Gly Tyr His Val Asp Phe Gly Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Arg Asp Phe Asp Ile Leu
195 200 205
Val Tyr Lys Ile Ser Gln Ser Asp Leu Asn Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ser Ser Ala Ile Pro Arg Thr Asn
245 250 255
Ser Ile Gly Lys Val Leu Asp Ala Lys Ala Asp Val Ser Asp Glu Met
260 265 270
Pro Asn Ser Glu Glu Ala Met Arg Arg Gly Glu Tyr Thr Val Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ser Thr Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Asn Ile Leu Arg Gln Ala Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Ile His Thr Glu Arg Ala Ala Leu
355 360 365
Arg Pro Ser Pro Ser Ser Gln Gly Thr Phe Ala Asp Trp Met Arg Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ala Ser Ala Glu Pro Ser
405 410
<210> 7
<211> 412
<212> PRT
<213> 小立碗藓(Physcomitrella patens)
<400> 7
Met Ala Val Ser Arg Glu Pro Glu Asn Val Ile Val Ser Arg Gln Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Leu Pro His Val Glu Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Val Gly Asn Lys Pro Val Tyr Gly Val Ala Ile Pro Thr Val
50 55 60
Asp Gly Ile Arg Arg Val Leu Glu Leu Ile Gly Ala Gly Lys Tyr Glu
65 70 75 80
Ser Gln Arg Val Leu Trp His Asn Leu Arg Glu Glu Pro Val Ile Tyr
85 90 95
Val Asn Gly Arg Pro Phe Val Leu Arg Glu Val Glu Arg Pro Phe Thr
100 105 110
Asn Leu Glu Tyr Thr Gly Ile Asn Arg Gln Arg Val Glu Gln Met Glu
115 120 125
Ala Arg Leu Lys Val Asp Val Leu Gln Glu Ala Ala Arg Tyr Ser Asn
130 135 140
Lys Ile Met Val Ser Asp Glu Leu Pro Asp Gly Gln Met Ile Asp Gln
145 150 155 160
Trp Glu Pro Val Gly Pro Asp Ser Ile Gln Thr Pro Phe Glu Val Tyr
165 170 175
Val Ser Leu Gln Ala Glu Gly Tyr Pro Val Asp Tyr Glu Arg Ile Pro
180 185 190
Ile Thr Asp Glu Lys Ser Pro Lys Glu Arg Asp Phe Asp Ser Leu Val
195 200 205
His Arg Phe Ser Gln Val Asp Val Glu Thr Lys Leu Val Phe Asn Cys
210 215 220
Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu
225 230 235 240
Ile His Leu Gln Arg Val Gly Ala Ser Gly Leu Pro Arg Ser Ser Ser
245 250 255
Met Gly Thr Ile His Glu Ala Arg Asp Glu Met Leu His Asp Val Pro
260 265 270
Asp Thr Glu Glu Ala Leu Arg Arg Gly Glu Tyr Thr Val Ile Arg Ser
275 280 285
Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp
290 295 300
Lys Val Ile Asp Ser Cys Ser Ala Met Gln Asn Leu Arg Glu Ala Ile
305 310 315 320
Ala Gly Tyr Arg Asn Ser Ile Val Arg Gln Ala Asp Glu Lys Lys Arg
325 330 335
Glu Ala Gly Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe
340 345 350
Leu Ile Cys Phe Ala Val Tyr Ile His Thr Asp Lys Thr Ser Leu Gly
355 360 365
Ser Ser His Arg Ser Gly Ala Gly Gly Phe Glu Gln Trp Met Arg Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ser Asn Thr Pro Val Gly
405 410
<210> 8
<211> 412
<212> PRT
<213> 小立碗藓(Physcomitrella patens)
<400> 8
Met Ala Val Ser Arg Glu Pro Glu Asp Val Ile Ile Ser Arg Asn Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Leu Pro His Val Glu Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Val Gly Asn Lys Pro Val Tyr Gly Val Ala Ile Pro Thr Val
50 55 60
Asp Gly Ile Arg Arg Ile Leu Glu Leu Ile Gly Ala Ser Lys Tyr Gly
65 70 75 80
Cys Gln Arg Val Leu Trp His Asn Leu Arg Glu Glu Pro Val Ile Tyr
85 90 95
Val Asn Gly Arg Pro Phe Val Leu Arg Glu Val Glu Arg Pro Phe Ser
100 105 110
Asn Leu Glu Tyr Thr Gly Ile Asn Arg Gln Arg Val Glu Gln Met Glu
115 120 125
Ser Arg Leu Lys Glu Asp Val Leu Leu Glu Ala Ala Arg Tyr Ala Asn
130 135 140
Lys Ile Met Val Ser Asp Glu Leu Pro Asp Gly Gln Met Ile Asp Gln
145 150 155 160
Trp Glu Pro Val Gly Pro Asn Ser Ile Gln Thr Pro Phe Glu Val Tyr
165 170 175
Glu Ser Leu Gln Ala Glu Gly Tyr Leu Val Asp Tyr Glu Arg Ile Pro
180 185 190
Ile Thr Asp Glu Lys Ser Pro Lys Val Arg Asp Phe Asp Ser Leu Val
195 200 205
Gln Arg Leu Ser Gln Val Glu Phe Gly Thr Lys Leu Val Phe Asn Cys
210 215 220
Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu
225 230 235 240
Ile His Phe Lys Arg Met Gly Ala Ser Gly Leu Pro Arg Ser Ser Ser
245 250 255
Met Gly Thr Ile His Glu Ala Arg Asp Glu Met Val Tyr Asp Val Pro
260 265 270
Asp Thr Glu Glu Ala Leu Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser
275 280 285
Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp
290 295 300
Lys Val Ile Asp Ser Cys Ser Ala Met Gln Asn Leu Arg Glu Ala Ile
305 310 315 320
Ala Ser Tyr Arg Ser Ser Ile Val Arg Gln Ala Asp Glu Lys Lys Arg
325 330 335
Ala Ala Gly Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe
340 345 350
Leu Ile Cys Phe Ala Val Tyr Ile His Thr Asp Lys Thr Cys Leu Gly
355 360 365
Ser Thr His Arg Pro Gly Gln Gly Gly Phe Glu Gln Trp Met Arg Asp
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ser Asn Thr Pro Val Gly
405 410
<210> 9
<211> 417
<212> PRT
<213> 二穗短柄草(Brachypodium distachyon)
<400> 9
Met Glu Val Gly Glu Met Asp Asp Val Gln Ala Val Gly Glu Gln Val
1 5 10 15
Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys
20 25 30
Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro His Ile
35 40 45
Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly
50 55 60
Val Ala Met Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile
65 70 75 80
Gly Ala Gln Lys Lys Gly Lys Gln Thr Arg Val Leu Trp His Ser Leu
85 90 95
Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg
100 105 110
Asp Val Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg
115 120 125
Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln
130 135 140
Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro
145 150 155 160
Asn Gly Gln Met Val Asp Gln Trp Glu Ser Val Met Ser Asp Thr Val
165 170 175
Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu
180 185 190
Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu
195 200 205
Gly Asp Phe Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu
210 215 220
Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr
225 230 235 240
Gly Met Val Ile Ser Thr Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser
245 250 255
Gly Ile Pro Arg Thr Ser Ser Ile Gly Lys Val Phe Tyr Ala Val Asn
260 265 270
Asp Val Asp Asp Phe Ser Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly
275 280 285
Glu Tyr Ala Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val
290 295 300
Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Ser Met
305 310 315 320
Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Ser Ser Thr Leu Arg
325 330 335
Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu
340 345 350
Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His
355 360 365
Ser Val Ser Ser Ala His Gln Ala Thr Ser Ser Glu Val Ser Phe Ser
370 375 380
Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu
385 390 395 400
Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro
405 410 415
Ser
<210> 10
<211> 440
<212> PRT
<213> 假稻属植物(Brachypodium distachyon)
<400> 10
Met Glu Glu Gln Ala Val Pro Glu Gln Val Ile Ser Thr Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met Pro Thr Met
50 55 60
Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln Lys Lys Gly
65 70 75 80
Lys Gln Thr His Val Leu Trp His Ser Leu Arg Glu Glu Pro Val Ile
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met
115 120 125
Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Ser Val Val Ser Asp Thr Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe Asp Asn Leu
195 200 205
Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ser Val Phe Glu Phe
245 250 255
Ser Tyr Phe Glu Val Cys Arg Val Leu Phe Thr Ala Gly Ile Pro Arg
260 265 270
Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp Asp
275 280 285
His Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser Val
290 295 300
Ile Arg Ser Leu Val Arg Val Leu Glu Arg Gly His Phe Val Ile Ala
305 310 315 320
Phe Asp Gln Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile
325 330 335
Asp Lys Cys Asp Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr
340 345 350
Arg Ser Asn Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ser
355 360 365
Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys
370 375 380
Phe Ala Val Tyr Val His Ser Val Cys Ser Val Gln Gln Ser Asn Phe
385 390 395 400
Ser Glu Glu Val Ser Phe Ser Asp Trp Met Arg Ala Arg Pro Glu Leu
405 410 415
Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu
420 425 430
Gly Tyr Ser Ser Ser Lys Ala Pro
435 440
<210> 11
<211> 410
<212> PRT
<213> 斑点野生稻(Oryza punctata)
<400> 11
Met Glu Gly Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Ser Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val His Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys
290 295 300
Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu Arg Glu Ala Ile Ala
305 310 315 320
Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
325 330 335
Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
340 345 350
Ile Cys Phe Ala Val Tyr Val His Ser Val Cys Ser Ala Gln Gln Ser
355 360 365
Lys Phe Ser Glu Glu Val Thr Phe Ser Asp Trp Met Arg Ala Arg Pro
370 375 380
Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
385 390 395 400
Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410
<210> 12
<211> 527
<212> PRT
<213> 穿心莲(Panicum hallii)
<400> 12
Met Pro Ile Pro Ser Ser Ala Leu Pro Pro Ile Arg Pro Ala Ser Pro
1 5 10 15
His Arg Pro Pro Pro Phe Ala Ser Ala Pro Pro His Pro Phe Pro Leu
20 25 30
Leu Leu Ala Ser Ala Ser Ala Ser Ala Pro Ala Pro Thr Pro Ser Ser
35 40 45
Ser Ser Ala Pro Arg Ser Leu Leu Val Leu Pro Pro Pro Pro Pro Pro
50 55 60
Pro Pro Arg Arg Pro Gly Pro Pro Ser Leu Arg Ala Val Ala Cys Ala
65 70 75 80
Gln Pro Arg Arg Gly Ser Arg Arg Cys Arg Ala Lys Leu Arg Gly Ala
85 90 95
Gly Leu Gly Val Pro Pro Leu Ala Ala Ala Gly Met Glu Ala Gly Glu
100 105 110
Met Glu Asp Val Arg Ala Ala Ala Ala Ala Glu Gln Val Ile Ser Ser
115 120 125
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
130 135 140
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
145 150 155 160
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
165 170 175
Pro Thr Met Glu Gly Ile Ala Asn Val Leu Asn His Ile Gly Ala Lys
180 185 190
Lys Lys Gly Lys Gln Thr Arg Ile Leu Trp His Ser Leu Arg Glu Glu
195 200 205
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
210 215 220
Lys Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
225 230 235 240
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
245 250 255
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Ser Gly Gln
260 265 270
Met Val Asp Gln Trp Glu Ser Val Val Ser Asp Thr Val Lys Thr Pro
275 280 285
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
290 295 300
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
305 310 315 320
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Leu Glu Thr Glu Ile
325 330 335
Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
340 345 350
Ile Ala Thr Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro
355 360 365
Arg Thr Gly Ser Ile Gly Lys Val Phe Tyr Ala Gly Asn Asp Val Asp
370 375 380
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ala
385 390 395 400
Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val Glu Gly Lys
405 410 415
Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Ser Met Gln Asn Leu
420 425 430
Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Ser Asp
435 440 445
Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu
450 455 460
Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His Ser Val Ser
465 470 475 480
Ser Ala His Gln Thr Lys Phe Ser Val Glu Val Ser Phe Ser Asp Trp
485 490 495
Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg
500 505 510
Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro Pro
515 520 525
<210> 13
<211> 199
<212> PRT
<213> 香蕉(Musa acuminata)
<400> 13
Met Asn Tyr Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys
1 5 10 15
Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro Gln Ile
20 25 30
Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu His Val His Gly
35 40 45
Val Ala Ile Pro Thr Ile Asp Gly Ile Gln Asn Val Leu Asn His Ile
50 55 60
Gly Ala Lys Gln Asp Leu Lys Lys Lys Gly Val Leu Trp His Asn Leu
65 70 75 80
Arg Glu Glu Pro Val Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg
85 90 95
Asp Val Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg
100 105 110
Ala Arg Leu Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Arg
115 120 125
Glu Ala Ala Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro
130 135 140
Asp Gly Gln Met Val Asp Gln Trp Glu Pro Val Met His Asn Ser Val
145 150 155 160
Lys Ala Pro Leu Glu Val Tyr Glu Glu Leu Gln Glu Glu Gly Tyr Leu
165 170 175
Val Asn Tyr Glu Arg Ile Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu
180 185 190
Gly Asp Phe Asp Asp Leu Val
195
<210> 14
<211> 186
<212> PRT
<213> 香蕉(Musa acuminata)
<400> 14
Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val
1 5 10 15
Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Thr Ser Ile
20 25 30
Gly Lys Val Phe Ala Ser Asp Gly Asp Val Thr Asp Tyr Gln Pro Asn
35 40 45
Ser Glu Glu Ser Ile Arg Arg Gly Glu Tyr Thr Val Ile Arg Ser Leu
50 55 60
Ile Arg Val Leu Glu Gly Gly Ala Glu Ala Lys Lys Gln Val Asp Lys
65 70 75 80
Val Ile Asp Lys Cys Asp Ser Met Gln Asn Leu Arg Glu Ala Ile Ala
85 90 95
Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
100 105 110
Ala Leu Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
115 120 125
Ile Cys Phe Ala Val Tyr Val His Thr Glu Arg Ala Ala Leu His Ser
130 135 140
Val Ser Ser Asp Gln Ile Ser Phe Ser Glu Trp Met Arg Ala Arg Pro
145 150 155 160
Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
165 170 175
Ala Leu Gly Tyr Ser Ser Leu Lys Pro Ser
180 185
<210> 15
<211> 416
<212> PRT
<213> 短花药野生稻(Oryza brachyantha)
<400> 15
Met Glu Gly Val Glu Val Glu Gln Val Ala Pro Glu Gln Val Ile Ser
1 5 10 15
Thr Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp
20 25 30
His Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly
35 40 45
Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala
50 55 60
Met Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala
65 70 75 80
Gln Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu
85 90 95
Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val
100 105 110
Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg
115 120 125
Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala
130 135 140
Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly
145 150 155 160
Gln Met Val Asp Gln Trp Glu Ser Val Ile Ser Asp Thr Val Lys Thr
165 170 175
Pro Leu Glu Val Tyr Glu Glu Leu Gln Cys Gln Gly Tyr Leu Val Asp
180 185 190
Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp
195 200 205
Phe Asp Asn Leu Val His Arg Ile Ser Gln Val Gly Ile Glu Thr Glu
210 215 220
Ile Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met
225 230 235 240
Val Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile
245 250 255
Pro Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val
260 265 270
Asp Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr
275 280 285
Ser Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val Glu Gly
290 295 300
Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Thr Met Gln Asn
305 310 315 320
Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Ala
325 330 335
Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu
340 345 350
Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His Ser Val
355 360 365
Cys Ser Ala Gln Gln Ser Asn Phe Ser Glu Glu Val Ser Phe Ser Asp
370 375 380
Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu
385 390 395 400
Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro Pro
405 410 415
<210> 16
<211> 324
<212> PRT
<213> 长雄蕊野生稻(Oryza longistaminata)
<400> 16
Met Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala
1 5 10 15
Gln Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu
20 25 30
Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val
35 40 45
Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Ile Leu Val Thr Asp Glu
50 55 60
Leu Pro Asn Gly Gln Met Val Asp Gln Trp Glu Ser Val Val Thr Asp
65 70 75 80
Thr Val Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly
85 90 95
Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro
100 105 110
Lys Glu Gly Asp Phe Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp
115 120 125
Ile Glu Thr Glu Ile Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr
130 135 140
Thr Thr Gly Met Val Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly
145 150 155 160
Ser Ser Gly Ile Pro Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser
165 170 175
Gly Asn Asp Val Asp Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu
180 185 190
Arg Gly Glu Tyr Ser Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly
195 200 205
Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp
210 215 220
Thr Met Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Ile
225 230 235 240
Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe
245 250 255
Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr
260 265 270
Val His Ser Val Cys Ser Ala Gln Gln Ser Asn Phe Ser Glu Glu Val
275 280 285
Ser Phe Ser Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu
290 295 300
Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser
305 310 315 320
Ser Lys Pro Ser
<210> 17
<211> 410
<212> PRT
<213> 巴蒂野生稻(Oryza barthii)
<400> 17
Met Glu Ala Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Thr Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys
290 295 300
Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu Arg Glu Ala Ile Ala
305 310 315 320
Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
325 330 335
Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
340 345 350
Ile Cys Phe Ala Val Tyr Val His Ser Val Cys Ser Ala Gln Gln Ser
355 360 365
Asn Phe Ser Glu Glu Val Ser Phe Ser Asp Trp Met Arg Ala Arg Pro
370 375 380
Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
385 390 395 400
Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410
<210> 18
<211> 410
<212> PRT
<213> 一年生普通野生稻(Oryza nivara)
<400> 18
Met Glu Ala Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Thr Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys
290 295 300
Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu Arg Glu Ala Ile Ala
305 310 315 320
Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
325 330 335
Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
340 345 350
Ile Cys Phe Ala Val Tyr Val His Ser Val Cys Ser Ala Gln Gln Ser
355 360 365
Asn Phe Ser Glu Glu Val Ser Phe Ser Asp Trp Met Arg Ala Arg Pro
370 375 380
Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
385 390 395 400
Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410
<210> 19
<211> 410
<212> PRT
<213> 陵水普通野生稻(Oryza rufipogon)
<400> 19
Met Glu Ala Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Thr Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys
290 295 300
Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu Arg Glu Ala Ile Ala
305 310 315 320
Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
325 330 335
Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
340 345 350
Ile Cys Phe Ala Val Tyr Val His Ser Val Cys Ser Ala Gln Gln Ser
355 360 365
Asn Phe Ser Glu Glu Val Ser Phe Ser Asp Trp Met Arg Ala Arg Pro
370 375 380
Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
385 390 395 400
Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410
<210> 20
<211> 410
<212> PRT
<213> 尼瓦拉野生稻(Oryza glumipatula)
<400> 20
Met Glu Ala Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Thr Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys
290 295 300
Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu Arg Glu Ala Ile Ala
305 310 315 320
Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
325 330 335
Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
340 345 350
Ile Cys Phe Ala Val Tyr Val His Ser Val Cys Ser Ala Gln Gln Ser
355 360 365
Asn Phe Ser Glu Glu Val Ser Phe Ser Asp Trp Met Arg Ala Arg Pro
370 375 380
Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
385 390 395 400
Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410
<210> 21
<211> 349
<212> PRT
<213> 乌拉尔图小麦(Triticum urartu)
<400> 21
Met Glu Val Gly Glu Met Asp Asp Val Gln Ala Val Gly Glu Gln Val
1 5 10 15
Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys
20 25 30
Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro His Ile
35 40 45
Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly
50 55 60
Val Ala Met Pro Thr Met Glu Gly Ile Val Asn Val Leu Asn His Ile
65 70 75 80
Gly Ala Gln Lys Lys Gly Lys Gln Thr Arg Val Leu Trp His Ser Leu
85 90 95
Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg
100 105 110
Asp Ala Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg
115 120 125
Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln
130 135 140
Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro
145 150 155 160
Asn Gly Gln Met Val Asp Gln Trp Glu Ser Val Met Ser Asp Thr Val
165 170 175
Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu
180 185 190
Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu
195 200 205
Gly Asp Phe Asp Asn Leu Leu Val Gly Asn Thr Phe Ser Arg Asp Cys
210 215 220
Ile Leu Arg Arg Gln Ser Ala Ile Ala Ile Ile Lys Ile Leu Arg Leu
225 230 235 240
Glu Ser Ile Pro Arg Thr Ser Ser Ile Gly Lys Val Phe Tyr Ala Gly
245 250 255
Asn Asp Leu Asp Asp Tyr Ser Pro Ser Ser Glu Glu Ala Ile Leu Arg
260 265 270
Gly Glu Tyr Ala Val Ile Arg Ser Leu Gly Gly Val Glu Gly Lys Arg
275 280 285
Gln Val Asp Lys Val Ile Asp Lys Cys Asp Ser Met Gln Asn Leu Arg
290 295 300
Glu Ala Ile Ala Thr Tyr Arg Asn Ser Thr Leu Arg Gln Pro Asp Glu
305 310 315 320
Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg
325 330 335
Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His Ser
340 345
<210> 22
<211> 415
<212> PRT
<213> 光稃稻(Oryza glaberrima)
<400> 22
Met Glu Ala Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Thr Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val Glu Gly Lys
290 295 300
Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu
305 310 315 320
Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp
325 330 335
Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu
340 345 350
Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His Ser Val Cys
355 360 365
Ser Ala Gln Gln Ser Asn Phe Ser Glu Glu Val Ser Phe Ser Asp Trp
370 375 380
Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg
385 390 395 400
Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410 415
<210> 23
<211> 415
<212> PRT
<213> 粳稻(Oryza sativa Japonica)
<400> 23
Met Glu Ala Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Thr Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val Glu Gly Lys
290 295 300
Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu
305 310 315 320
Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp
325 330 335
Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu
340 345 350
Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His Ser Val Cys
355 360 365
Ser Ala Gln Gln Ser Asn Phe Ser Glu Glu Val Ser Phe Ser Asp Trp
370 375 380
Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg
385 390 395 400
Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410 415
<210> 24
<211> 417
<212> PRT
<213> 普通小麦(Triticum aestivum)
<400> 24
Met Glu Val Gly Glu Met Asp Asp Val Gln Ala Val Gly Glu Gln Val
1 5 10 15
Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys
20 25 30
Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro His Ile
35 40 45
Asp Gly Ala Pro Xaa Xaa Xaa Xaa Ala Gly Ser Leu Arg Val His Gly
50 55 60
Val Ala Met Pro Thr Met Glu Gly Ile Val Asn Val Leu Asn His Ile
65 70 75 80
Gly Ala Gln Lys Lys Gly Lys Gln Thr Arg Val Leu Trp His Ser Leu
85 90 95
Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg
100 105 110
Asp Ala Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg
115 120 125
Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln
130 135 140
Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro
145 150 155 160
Asn Gly Gln Met Val Asp Gln Trp Glu Ser Val Met Ser Asp Thr Val
165 170 175
Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu
180 185 190
Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu
195 200 205
Gly Asp Phe Asp Asn Leu Val His Arg Ile Ser Gln Val Asp Met Glu
210 215 220
Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr
225 230 235 240
Gly Met Val Ile Ser Thr Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser
245 250 255
Gly Ile Pro Arg Thr Ser Ser Ile Gly Lys Val Phe Tyr Ala Gly Asn
260 265 270
Asp Val Asp Asp Tyr Ser Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly
275 280 285
Glu Tyr Ala Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val
290 295 300
Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Ser Met
305 310 315 320
Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Thr Leu Arg
325 330 335
Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu
340 345 350
Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His
355 360 365
Ser Val Ser Ser Ala His Gln Ala Thr Ser Ser Gly Val Asn Phe Ser
370 375 380
Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu
385 390 395 400
Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro
405 410 415
Thr
<210> 25
<211> 417
<212> PRT
<213> 普通小麦(Triticum aestivum)
<400> 25
Met Glu Val Gly Glu Met Asp Asp Val Gln Ala Val Gly Glu Gln Val
1 5 10 15
Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys
20 25 30
Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro His Ile
35 40 45
Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly
50 55 60
Val Ala Met Pro Thr Met Glu Gly Ile Val Asn Val Leu Asn His Ile
65 70 75 80
Gly Ala Gln Lys Lys Gly Lys Gln Thr Arg Val Leu Trp His Ser Leu
85 90 95
Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg
100 105 110
Asp Ala Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg
115 120 125
Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln
130 135 140
Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro
145 150 155 160
Asn Gly Gln Met Val Asp Gln Trp Glu Ser Val Met Ser Asp Thr Val
165 170 175
Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu
180 185 190
Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu
195 200 205
Gly Asp Phe Asp Asn Leu Val His Arg Ile Ser Gln Val Asp Met Glu
210 215 220
Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr
225 230 235 240
Gly Met Val Ile Ser Thr Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser
245 250 255
Gly Ile Pro Arg Thr Ser Ser Ile Gly Lys Val Phe Tyr Ala Gly Asn
260 265 270
Asp Val Asp Asp Tyr Ser Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly
275 280 285
Glu Tyr Ala Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val
290 295 300
Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Ser Met
305 310 315 320
Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Thr Leu Arg
325 330 335
Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu
340 345 350
Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His
355 360 365
Ser Val Ser Ser Ala His Gln Ala Thr Ser Ser Gly Val His Phe Ser
370 375 380
Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu
385 390 395 400
Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro
405 410 415
Thr
<210> 26
<211> 417
<212> PRT
<213> 普通小麦(Triticum aestivum)
<400> 26
Met Glu Val Gly Glu Met Asp Asp Val Gln Ala Val Gly Glu Gln Val
1 5 10 15
Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys
20 25 30
Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro His Ile
35 40 45
Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly
50 55 60
Val Ala Met Pro Thr Met Glu Gly Ile Val Asn Val Leu Asn His Ile
65 70 75 80
Gly Ala Gln Lys Lys Gly Lys Gln Thr Arg Val Leu Trp His Ser Leu
85 90 95
Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg
100 105 110
Asp Ala Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg
115 120 125
Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln
130 135 140
Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro
145 150 155 160
Asn Gly Gln Met Val Asp Gln Trp Glu Ser Val Met Ser Asp Thr Val
165 170 175
Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu
180 185 190
Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu
195 200 205
Gly Asp Phe Asp Asn Leu Val His Arg Ile Ser Gln Val Asp Met Glu
210 215 220
Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr
225 230 235 240
Gly Met Val Ile Ser Thr Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser
245 250 255
Gly Ile Pro Arg Thr Ser Ser Ile Gly Lys Val Phe Tyr Ala Gly Asn
260 265 270
Asp Leu Asp Asp Tyr Ser Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly
275 280 285
Glu Tyr Ala Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val
290 295 300
Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Ser Met
305 310 315 320
Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Thr Leu Arg
325 330 335
Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu
340 345 350
Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His
355 360 365
Ser Val Ser Ser Ala His Gln Ala Thr Ser Ser Gly Val Ser Phe Ser
370 375 380
Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu
385 390 395 400
Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro
405 410 415
Thr
<210> 27
<211> 420
<212> PRT
<213> 小米(Setaria italica)
<400> 27
Met Glu Ala Gly Glu Met Glu Asp Val Arg Ala Ala Ala Ala Ala Glu
1 5 10 15
Gln Val Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile
20 25 30
Leu Lys Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro
35 40 45
Gln Ile Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val
50 55 60
His Gly Val Ala Met Pro Thr Met Glu Gly Ile Ala Asn Val Leu Asn
65 70 75 80
His Ile Gly Ala Lys Lys Lys Gly Lys Gln Thr Arg Ile Leu Trp His
85 90 95
Ser Leu Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val
100 105 110
Leu Arg Asp Val Glu Lys Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile
115 120 125
Asn Arg Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile
130 135 140
Leu Gln Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu
145 150 155 160
Leu Pro Ser Gly Gln Met Val Asp Gln Trp Glu Ser Val Val Ser Asp
165 170 175
Thr Val Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly
180 185 190
Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro
195 200 205
Lys Glu Gly Asp Phe Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp
210 215 220
Leu Glu Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr
225 230 235 240
Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr Leu Asn Arg Ile Gly
245 250 255
Ala Ser Gly Ile Pro Arg Thr Gly Ser Ile Gly Lys Val Phe Tyr Ala
260 265 270
Gly Asn Asp Val Asp Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu
275 280 285
Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly
290 295 300
Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp
305 310 315 320
Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Ile
325 330 335
Leu Arg Gln Ser Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe
340 345 350
Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr
355 360 365
Val His Ser Val Ser Ser Ala His Gln Thr Thr Phe Ser Val Glu Val
370 375 380
Ser Phe Ser Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu
385 390 395 400
Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser
405 410 415
Ser Lys Pro Pro
420
<210> 28
<211> 468
<212> PRT
<213> 几内亚山药(Dioscorea rotundata)
<400> 28
Met Thr Lys Val Pro Ser Ser Ser Ala Pro Ser Ile Ser Ala Ser Ile
1 5 10 15
Gly Ser Val Cys Asp Glu Pro Glu His Val Met Asn Cys Arg Gly Gly
20 25 30
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
35 40 45
Cys Gln Asn Lys Arg Leu Thr Pro Gln Ile Glu Gly Ala Pro Asn Tyr
50 55 60
Arg Gln Phe Ala Ile Asp Thr Leu Val Asn Pro Glu Arg Phe Gln Leu
65 70 75 80
Thr Gly Val Val Leu Ser Gln Val Gly Asp Asn Ile Leu Cys Lys Phe
85 90 95
Ala Ser Pro Lys Leu Ser Gly Ser Thr Pro Val Ala Gly Phe Leu Arg
100 105 110
Val His Gly Val Ala Ile Pro Thr Ile Asp Gly Ile Arg Asn Val Leu
115 120 125
Asx His Ile Gly Ala Lys Gly Met His Lys Gln Gln Arg Val Leu Trp
130 135 140
His Asn Leu Arg Glu Glu Pro Val Val Tyr Ile Asn Gly Arg Pro Phe
145 150 155 160
Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly
165 170 175
Ile Asn Arg Ala Arg Val Glu Leu Met Glu Ser Arg Leu Lys Asp Asp
180 185 190
Ile Leu Met Glu Ala Thr Gln Tyr Gly Asn Lys Ile Leu Val Thr Asp
195 200 205
Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp Glu Pro Val Met Arg
210 215 220
Asp Ser Val Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln Leu Glu
225 230 235 240
Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ser
245 250 255
Pro Lys Glu Arg Asp Phe Asp Asn Leu Leu Cys Arg Ile Ser Gln Ala
260 265 270
Asp Ile Asp Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg
275 280 285
Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr Leu Asn Arg Thr
290 295 300
Gly Ala Ser Gly Ile His Asp Thr Asn Phe Ile Gly Glu Val Asp Ser
305 310 315 320
Val Ala Asp Val Thr Asp Asn Ser Thr Asn Ser Glu Glu Ala Ile Arg
325 330 335
Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Ile Arg Val Leu Glu Gly
340 345 350
Gly Ala Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Gln Cys Asp
355 360 365
Ser Met Gln Asn Leu Arg Glu Ala Ile Gly Val Tyr Arg Asn Ser Ile
370 375 380
Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe
385 390 395 400
Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr
405 410 415
Met His Thr Glu Arg Met Leu Val His Gly Ile Ser Ala Glu Gln Arg
420 425 430
Ser Phe Ser Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu
435 440 445
Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser
450 455 460
Gln Lys Pro His
465
<210> 29
<211> 420
<212> PRT
<213> 高粱(Sorghum bicolor)
<400> 29
Met Asp Ala Gly Glu Met Glu Asp Val Arg Ala Ala Ala Ala Ala Glu
1 5 10 15
Glu Val Ile Ser Ser Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile
20 25 30
Leu Lys Ser Asp His Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro
35 40 45
Gln Ile Asp Gly Ala Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val
50 55 60
His Gly Val Ala Met Pro Thr Met Glu Gly Ile Ala Asn Val Leu Asn
65 70 75 80
His Ile Gly Ala His Lys Lys Gly Lys Gln Thr Arg Ile Leu Trp His
85 90 95
Ser Leu Arg Glu Glu Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val
100 105 110
Leu Arg Asp Val Glu Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile
115 120 125
Asn Arg Glu Arg Val Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile
130 135 140
Leu Gln Glu Ala Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu
145 150 155 160
Leu Pro Ser Gly Gln Met Val Asp Gln Trp Glu Ser Val Glu Tyr Asp
165 170 175
Thr Val Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln Gln Gln Gly
180 185 190
Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro
195 200 205
Lys Glu Gly Asp Phe Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp
210 215 220
Ile Glu Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg Gly Arg Thr
225 230 235 240
Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr Leu Asn Arg Ile Gly
245 250 255
Ala Ser Gly Ile Pro Arg Thr Gly Ser Ile Gly Lys Val Phe Tyr Ala
260 265 270
Gly Asn Asp Val Asp Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu
275 280 285
Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly
290 295 300
Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp
305 310 315 320
Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Asn Asn Ile
325 330 335
Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe
340 345 350
Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr
355 360 365
Val His Ser Val Ser Ser Ala His Gln Thr Thr Leu Ser Val Glu Val
370 375 380
Ser Phe Ser Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu
385 390 395 400
Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser
405 410 415
Ser Lys Pro Pro
420
<210> 30
<211> 475
<212> PRT
<213> 大麦(Hordeum vulgare)
<400> 30
Pro Gln Ser Asn Arg Ile Val Phe Phe Ser Pro Thr Val Ser Leu Pro
1 5 10 15
Pro Leu Pro Pro Ser Leu Pro Ser Leu Arg Arg Val Leu Ser Ala Ser
20 25 30
Ala Ser Arg Ala Ser Pro Leu Cys Arg Cys Leu Arg Ser Arg Cys Ala
35 40 45
Val Arg Gly Gly Gly Phe Val Glu Asp Trp Met Glu Val Gly Glu Met
50 55 60
Asp Asp Val Leu Ala Val Gly Glu Gln Val Ile Ser Ser Arg Gly Gly
65 70 75 80
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
85 90 95
Cys Gln Asn Lys Arg Leu Thr Pro His Ile Asp Gly Ala Pro Asn Tyr
100 105 110
Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met Pro Thr Met
115 120 125
Glu Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Gln Lys Lys Gly
130 135 140
Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu Pro Val Ile
145 150 155 160
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Ala Glu Arg Pro Phe
165 170 175
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met
180 185 190
Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser Arg Tyr Gly
195 200 205
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln Met Val Asp
210 215 220
Gln Trp Glu Ser Val Ile Ser Asp Thr Val Lys Thr Pro Leu Glu Val
225 230 235 240
Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr Glu Arg Val
245 250 255
Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe Asp Asn Leu
260 265 270
Val Arg Arg Ile Ser Glu Val Asp Met Glu Thr Glu Ile Val Phe Asn
275 280 285
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ser Thr
290 295 300
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Ser
305 310 315 320
Ser Ile Gly Lys Val Phe Tyr Ala Gly Asn Asp Val Asp Asp Tyr Ser
325 330 335
Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ala Val Ile Arg
340 345 350
Ser Leu Val Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
355 360 365
Asp Lys Val Ile Asp Lys Cys Asp Ser Met Gln Asn Leu Arg Glu Ala
370 375 380
Ile Ala Thr Tyr Arg Asn Ser Thr Leu Arg Gln Pro Asp Glu Met Lys
385 390 395 400
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
405 410 415
Phe Leu Ile Cys Phe Ala Val Tyr Val His Ser Val Ser Ser Ala His
420 425 430
Gln Ala Thr Ser Ser Gly Val Ser Phe Ser Asp Trp Met Arg Ala Arg
435 440 445
Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met
450 455 460
Gly Ala Leu Gly Tyr Ser Ser Ser Lys Leu Thr
465 470 475
<210> 31
<211> 415
<212> PRT
<213> 籼稻(Oryza sativa Indica)
<400> 31
Met Glu Ala Val Glu Glu Gln Val Ala Pro Glu Gln Val Ile Ser Thr
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Gly Ser Leu Arg Val His Gly Val Ala Met
50 55 60
Pro Thr Met Lys Gly Ile Val Asn Val Leu Asn His Ile Gly Ala Lys
65 70 75 80
Lys Lys Gly Lys Gln Thr Gln Val Leu Trp His Ser Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val
115 120 125
Glu Gln Met Glu Phe Arg Leu Lys Glu Asp Ile Leu Gln Glu Ala Ser
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asn Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Ser Val Val Thr Asp Thr Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Glu Leu Gln His Gln Gly Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ala Pro Lys Glu Gly Asp Phe
195 200 205
Asp Asn Leu Val Arg Arg Ile Ser Gln Val Asp Ile Glu Thr Glu Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ser Ser Gly Ile Pro
245 250 255
Arg Thr Ser Ser Ile Gly Lys Val Phe His Ser Gly Asn Asp Val Asp
260 265 270
Asp Tyr Met Pro Ser Ser Glu Glu Ala Ile Leu Arg Gly Glu Tyr Ser
275 280 285
Val Ile Arg Ser Leu Val Arg Val Leu Glu Gly Gly Val Glu Gly Lys
290 295 300
Arg Gln Val Asp Lys Val Ile Asp Lys Cys Asp Thr Met Gln Asn Leu
305 310 315 320
Arg Glu Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp
325 330 335
Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu
340 345 350
Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Val His Ser Val Cys
355 360 365
Ser Ala Gln Gln Ser Asn Phe Ser Glu Glu Val Ser Phe Ser Asp Trp
370 375 380
Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg
385 390 395 400
Arg Asp Pro Met Gly Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410 415
<210> 32
<211> 413
<212> PRT
<213> 大豆(Glycine max)
<400> 32
Met Thr Ser Ile Pro Lys Glu Pro Glu Glu Val Met Lys Arg Arg Gly
1 5 10 15
Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys His Asn Lys Arg Leu His Pro His Ile Asp Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Glu Ser Leu His Val His Gly Val Ala Ile Pro Thr
50 55 60
Thr Asp Gly Ile Arg Asn Val Leu Lys His Ile Gly Ala Arg Ala Glu
65 70 75 80
Gly Lys Lys Ala Gln Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val
85 90 95
Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro
100 105 110
Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln
115 120 125
Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr
130 135 140
Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val
145 150 155 160
Asp Gln Trp Glu Ser Val Ser Cys Asn Ser Val Lys Ala Pro Leu Glu
165 170 175
Val Tyr Gln Glu Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg
180 185 190
Val Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Arg Asp Phe Asp Ile
195 200 205
Leu Val His Lys Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Ile Phe
210 215 220
Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala
225 230 235 240
Thr Leu Phe Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser
245 250 255
Asn Ser Val Gly Arg Val Ser Gln Cys Leu Thr Asn Val Ala Asp Tyr
260 265 270
Ile Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile
275 280 285
Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln
290 295 300
Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu
305 310 315 320
Ala Ile Gly Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met
325 330 335
Lys Lys Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr
340 345 350
Tyr Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Met Ala Thr
355 360 365
Leu Cys Ser Cys Ser Ala Asp His Ser Ser Phe Thr Asp Trp Met Arg
370 375 380
Asn Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asn
385 390 395 400
Pro Met Gly Ala Leu Gly Tyr Ser Ser Leu Lys Pro Ser
405 410
<210> 33
<211> 413
<212> PRT
<213> 大豆(Glycine max)
<400> 33
Met Thr Ser Ile Pro Lys Glu Pro Glu Glu Val Met Lys Arg Arg Gly
1 5 10 15
Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys His Asn Lys Arg Leu His Pro His Ile Asp Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Glu Ser Leu Leu Val His Gly Val Ala Ile Pro Thr
50 55 60
Thr Asp Gly Ile Arg Asn Val Leu Lys His Ile Gly Ala Arg Ala Glu
65 70 75 80
Gly Lys Lys Ala Gln Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val
85 90 95
Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro
100 105 110
Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln
115 120 125
Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr
130 135 140
Glu Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val
145 150 155 160
Asp Gln Trp Glu Ser Val Ser Cys Asn Ser Val Lys Thr Pro Leu Glu
165 170 175
Val Tyr Gln Glu Leu Gln Val Ala Gly Tyr Leu Val Asp Tyr Glu Arg
180 185 190
Val Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Trp Asp Phe Asp Ile
195 200 205
Leu Val His Lys Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Ile Phe
210 215 220
Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala
225 230 235 240
Thr Leu Phe Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser
245 250 255
Asn Ser Val Gly Arg Val Ser Gln Cys Leu Thr Asn Val Ala Asp Tyr
260 265 270
Ile Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile
275 280 285
Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln
290 295 300
Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu
305 310 315 320
Ala Ile Gly Thr Tyr Arg Asn Ser Ile Leu Trp Gln Pro Asp Glu Met
325 330 335
Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr
340 345 350
Tyr Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Met Ala Thr
355 360 365
Leu Cys Ser Ser Ser Val Asp Arg Ser Ser Phe Thr Asp Trp Met Arg
370 375 380
Asp Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asn
385 390 395 400
Pro Met Gly Ala Leu Gly Tyr Ser Asn Leu Lys Pro Ser
405 410
<210> 34
<211> 409
<212> PRT
<213> 大豆(Glycine max)
<400> 34
Met Ser Gln Ile Pro Lys Glu Pro Glu Gln Val Met Lys Met Arg Gly
1 5 10 15
Gly Cys Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Ser Asp Ser Leu His Val His Gly Val Ala Ile Pro
50 55 60
Thr Val His Gly Ile Arg Asn Val Leu Asn His Ile Gly Ala Arg Leu
65 70 75 80
Lys Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Leu Ala Tyr Ile Asn
85 90 95
Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn Leu
100 105 110
Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu Ala Arg
115 120 125
Leu Lys Glu Asp Ile Leu Ala Glu Ala Ala Arg Tyr Gly Asn Lys Ile
130 135 140
Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp Glu
145 150 155 160
Pro Val Ser Cys Asp Ser Val Lys Thr Pro Leu Glu Val Tyr Glu Glu
165 170 175
Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr
180 185 190
Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu Val Asn Lys
195 200 205
Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Val Phe Asn Cys Gln Met
210 215 220
Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr
225 230 235 240
Leu Asn Arg Ile Gly Ala Ser Gly Phe Pro Arg Ser Asn Ser Ile Gly
245 250 255
Arg Ile Phe Gln Ser Met Thr Asn Val Ala Asp His Leu Pro Asn Ser
260 265 270
Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Ile
275 280 285
Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val
290 295 300
Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr
305 310 315 320
Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala
325 330 335
Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile
340 345 350
Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu Arg Ser Asn
355 360 365
Thr Ala Asp His Cys Ser Phe Ala Asp Trp Met Arg Ala Arg Pro Glu
370 375 380
Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala
385 390 395 400
Leu Gly Tyr Ser Ser Leu Lys Pro Ser
405
<210> 35
<211> 409
<212> PRT
<213> 大豆(Glycine max)
<400> 35
Met Ser Gln Ile Pro Lys Glu Pro Glu Gln Val Met Lys Met Arg Gly
1 5 10 15
Gly Cys Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Ser Asp Ser Leu His Val His Gly Val Ala Ile Pro
50 55 60
Thr Ile His Gly Ile Arg Asn Val Leu Asn His Ile Gly Ala Arg Leu
65 70 75 80
Lys Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Leu Ala Tyr Ile Asn
85 90 95
Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn Leu
100 105 110
Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu Ala Arg
115 120 125
Leu Lys Glu Asp Ile Leu Val Glu Ala Ala Arg Tyr Gly Asn Lys Ile
130 135 140
Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp Glu
145 150 155 160
Pro Val Ser Cys Asp Ser Val Lys Thr Pro Leu Glu Val Tyr Glu Glu
165 170 175
Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr
180 185 190
Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu Val Asn Lys
195 200 205
Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Ile Phe Asn Cys Gln Met
210 215 220
Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr
225 230 235 240
Leu Asn Arg Ile Gly Ala Ser Gly Phe Pro Arg Ser Asn Ser Ile Gly
245 250 255
Arg Ile Phe Gln Ser Met Thr Asn Val Ala Asp His Leu Pro Asn Ser
260 265 270
Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Ile
275 280 285
Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val
290 295 300
Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr
305 310 315 320
Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala
325 330 335
Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile
340 345 350
Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu Arg Ser Asn
355 360 365
Thr Ala Asp His Cys Ser Phe Ala Asp Trp Met Arg Ala Arg Pro Glu
370 375 380
Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala
385 390 395 400
Leu Gly Tyr Ser Ser Leu Lys Pro Ser
405
<210> 36
<211> 410
<212> PRT
<213> 白花羽扇豆(Lupinus angustifoliu)
<400> 36
Met Ser Ile Ile Pro Lys Glu Pro Glu Gln Val Met Lys Met Arg Gly
1 5 10 15
Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Thr Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Glu Ser Leu His Val His Gly Val Ala Ile Pro Thr
50 55 60
Ile Asp Gly Ile Arg Asn Val Leu Asn Arg Val Gly Ala Gln Leu His
65 70 75 80
Ala Lys Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Leu Val Tyr Ile
85 90 95
Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn
100 105 110
Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu Asp
115 120 125
Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr Gly Asn Lys
130 135 140
Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp
145 150 155 160
Glu Pro Val Ser Ser Ser Ser Val Lys Thr Pro Leu Glu Val Tyr Glu
165 170 175
Glu Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Val
180 185 190
Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu Val Gln
195 200 205
Lys Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Ile Phe Asn Cys Gln
210 215 220
Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val
225 230 235 240
Tyr Phe Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser Asn Ser Ile
245 250 255
Gly Arg Ile Ser Gln Phe Met Thr Asn Val Ala Asp Arg Leu Pro Asn
260 265 270
Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Val Val Ile Arg Ser Leu
275 280 285
Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys
290 295 300
Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile Ala
305 310 315 320
Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
325 330 335
Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
340 345 350
Ile Cys Phe Ala Val Tyr Leu His Ser Glu Arg Val Val Leu Leu Ser
355 360 365
Ser Thr Ser Thr Gln Ser Ser Phe Ala Asp Trp Met Arg Ala Arg Pro
370 375 380
Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
385 390 395 400
Ala Leu Gly Tyr Ser Ser Ser Lys Pro Ser
405 410
<210> 37
<211> 407
<212> PRT
<213> 白花羽扇豆(Lupinus angustifoliu)
<400> 37
Met Ala Lys Glu Pro Glu Gln Val Met Lys Met Arg Lys Gly Ser Val
1 5 10 15
Leu Gly Lys Lys Thr Ile Leu Lys Thr Asp His Phe Pro Gly Cys Gln
20 25 30
Asn Lys Arg Leu Phe Pro Gln Ile Asp Gly Ala Pro Asn Tyr Arg Gln
35 40 45
Ala Glu Ser Leu His Val His Gly Val Ala Ile Pro Thr Ile Asp Gly
50 55 60
Ile Arg Asn Val Leu Lys His Ile Gly Ala Gln Phe Gln Ala Lys Val
65 70 75 80
Leu Trp Ile Ser Leu Arg Glu Glu Pro Leu Val Tyr Val Asn Gly Arg
85 90 95
Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn Leu Glu Tyr
100 105 110
Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu Asp Arg Leu Lys
115 120 125
Glu Asp Ile Leu Met Glu Ala Glu Arg Tyr Glu Asn Lys Ile Leu Val
130 135 140
Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Leu Trp Glu Pro Val
145 150 155 160
Ser Ser Asn Ser Val Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu Gln
165 170 175
Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Val Thr Asp Glu
180 185 190
Lys Ser Pro Lys Glu Arg Asp Phe Asp Ile Leu Val Gln Lys Ile Ser
195 200 205
Gln Ala Asp Val Asn Thr Glu Ile Val Phe Asn Cys Gln Met Gly Arg
210 215 220
Gly Arg Thr Thr Thr Gly Met Val Val Ala Thr Leu Val Tyr Phe Asn
225 230 235 240
Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser Asn Ser Ile Gly Arg Ile
245 250 255
Ser Gln Phe Met Thr Asn Val Ala Asp Arg Leu Pro Asn Ser Glu Glu
260 265 270
Ala Ile Arg Arg Gly Glu Tyr Val Val Ile Arg Ser Leu Ile Arg Val
275 280 285
Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp
290 295 300
Met Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg
305 310 315 320
Ser Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ser Leu
325 330 335
Phe Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Ile Leu Ile Cys Phe
340 345 350
Ala Val Tyr Leu His Ser Glu Arg Ala Val Leu Leu Ser Ser Thr Ala
355 360 365
Thr Gln Ser Ser Phe Ala Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr
370 375 380
Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly
385 390 395 400
Tyr Ser Ser Ser Lys Pro Ser
405
<210> 38
<211> 411
<212> PRT
<213> 棉花(Gossypium raimondii)
<400> 38
Met Ser Ile Pro Lys Glu Leu Glu Gln Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Val Gly Ile His Asn Val Leu Lys His Ile Gly Ala Gln Lys Gly Gly
65 70 75 80
Lys Ala His Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val Val Tyr
85 90 95
Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser
100 105 110
Asn Leu Glu Tyr Thr Gly Ile Asn Arg Asp Arg Val Glu Gln Met Glu
115 120 125
Ala Arg Leu Lys Glu Asp Ile Leu Leu Glu Ala Ala Arg Tyr Gly Asn
130 135 140
Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln
145 150 155 160
Trp Glu Arg Val Ser Cys Asp Ser Val Lys Thr Pro Leu Glu Val Tyr
165 170 175
Glu Lys Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro
180 185 190
Ile Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Val Val
195 200 205
Asn Lys Ile Ser Gln Ala Asp Ile Ser Thr Glu Val Val Phe Asn Cys
210 215 220
Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu
225 230 235 240
Ala Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Asn Asp Ser
245 250 255
Ile Gly Arg Val Ser Asp Tyr Ala Ser Asn Val Thr Asp Asn Leu Pro
260 265 270
Asn Ser Glu Asp Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser
275 280 285
Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp
290 295 300
Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile
305 310 315 320
Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg
325 330 335
Glu Ala Ala Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe
340 345 350
Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu Arg
355 360 365
Ser Ser Ser Ser Gly His Thr Ser Phe Ala Asp Trp Met Lys Ala Arg
370 375 380
Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met
385 390 395 400
Gly Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
405 410
<210> 39
<211> 412
<212> PRT
<213> 棉花(Gossypium raimondii)
<400> 39
Met Ser Ile Pro Lys Glu Ile Glu Gln Val Met Lys Met Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Val Gly Ile Gln Asn Val Leu Asn His Ile Gly Ala Gln Lys Asp Gly
65 70 75 80
Lys Gln Ala His Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly His Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Asp Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Glu Met Val Asp
145 150 155 160
Gln Trp Glu Gln Val Ser Cys Asn Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Leu Ala Gly Tyr Leu Val Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu
195 200 205
Val Asn Lys Ile Ser Gln Ala Asp Ile Ser Thr Glu Val Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Asn
245 250 255
Ser Ile Gly Arg Val Phe Asn Ser Gly Ser Asn Ile Thr Asn Asn Leu
260 265 270
Pro Asn Ser Glu Glu Ala Ile Cys Arg Gly Glu Tyr Thr Leu Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu
355 360 365
Arg Ser Ser Ser Phe Asp His Thr Ser Phe Ser Asp Trp Met Lys Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Ile His Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ala Ser Leu Asn Pro Ser
405 410
<210> 40
<211> 412
<212> PRT
<213> 棉花(Gossypium raimondii)
<400> 40
Met Ser Ile Pro Lys Glu Leu Glu Gln Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Val Gly Ile Gln Asn Val Leu Lys His Ile Gly Ala Gln Lys Asp Gly
65 70 75 80
Lys Gln Ala Arg Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Met Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Asp Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Leu Trp Glu Arg Val Ser Cys Asp Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Leu Lys Gly Tyr Leu Val Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Met Asp Phe Asp Ile Leu
195 200 205
Val Asn Lys Leu Ser Gln Ala Asp Ile Arg Thr Glu Val Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Asn
245 250 255
Ser Ile Gly Arg Val Ser Glu Ser Gly Ser Asn Val Thr Asp Asn Leu
260 265 270
Pro Asn Ser Lys Glu Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Gln Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Met Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu
355 360 365
His Ser Ser Ser Ser Asn His Thr Ser Phe Ala Asp Trp Met Lys Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Arg Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
405 410
<210> 41
<211> 414
<212> PRT
<213> 蒺藜苜蓿(Medicago truncatula)
<400> 41
Met Ser Ile Pro Lys Glu Pro Glu Glu Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Arg Pro Asn Ile Glu Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Lys Leu His Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Asp Gly Ile Arg Asn Val Leu Lys His Ile Gly Ala Glu Ile Glu Gly
65 70 75 80
Glu Asn Lys Val His Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val
85 90 95
Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro
100 105 110
Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln
115 120 125
Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Asn Glu Ala Ala Arg Tyr
130 135 140
Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val
145 150 155 160
Asp Gln Trp Glu Ser Val Ser Cys Asn Ser Val Lys Thr Pro Leu Glu
165 170 175
Val Tyr Gln Glu Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg
180 185 190
Val Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Gln Asp Phe Asp Ile
195 200 205
Leu Val His Lys Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Ile Phe
210 215 220
Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala
225 230 235 240
Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser
245 250 255
Asn Ser Val Gly Thr Val Ser Gln Cys Leu Thr Asn Val Pro Asp His
260 265 270
Met Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile
275 280 285
Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Asp Gly Lys Arg Gln
290 295 300
Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu
305 310 315 320
Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met
325 330 335
Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr
340 345 350
Tyr Phe Leu Ile Cys Phe Thr Val Tyr Ile His Ser Glu Met Ala Ala
355 360 365
Leu Gln Ala Arg Ser Ala Ala Ser His Thr Ser Phe Ala Asp Trp Met
370 375 380
Arg Ala Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg
385 390 395 400
Asp Pro Met Gly Ala Leu Gly Tyr Ser Gly Leu Lys Pro Ser
405 410
<210> 42
<211> 411
<212> PRT
<213> 蒺藜苜蓿(Medicago truncatula)
<400> 42
Met Ala Arg Val Val Lys Glu Pro Glu Glu Val Met Arg Met Arg Gly
1 5 10 15
Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Glu Gly Ala Pro Asn
35 40 45
Tyr Arg Arg Ala Asp Ser Leu His Val His Gly Val Ala Ile Pro Thr
50 55 60
Val Asp Gly Ile Arg Asn Val Leu Asn His Ile Arg Asn Arg Gln Asn
65 70 75 80
Lys Gln Gln Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Leu Val Tyr
85 90 95
Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser
100 105 110
Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu
115 120 125
Ala Arg Leu Lys Glu Asp Ile Leu Ser Glu Ala Glu Arg Tyr Gly Tyr
130 135 140
Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln
145 150 155 160
Trp Glu Pro Val Ser Cys Asp Ser Val Lys Thr Pro Leu Glu Val Tyr
165 170 175
Glu Glu Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro
180 185 190
Ile Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu Val
195 200 205
His Lys Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Ile Phe Asn Cys
210 215 220
Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu
225 230 235 240
Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser Asn Ser
245 250 255
Met Gly Arg Ile Phe Gln Ser Met Thr Asn Ala Ala Asp His Leu Pro
260 265 270
Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser
275 280 285
Leu Val Arg Val Leu Glu Gly Gly Val Asp Gly Lys Arg Gln Val Asp
290 295 300
Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile
305 310 315 320
Gly Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Lys
325 330 335
Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe
340 345 350
Leu Ile Cys Phe Ala Val Tyr Ile Asn Ser Glu Arg Asp Ile Leu Leu
355 360 365
Ser Ser Thr Ala Gly Gln Ser Ser Phe Ser Asn Trp Met Arg Ala Arg
370 375 380
Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met
385 390 395 400
Gly Ala Leu Gly Tyr Ser Ser Leu Lys Pro Ser
405 410
<210> 43
<211> 414
<212> PRT
<213> 鼠耳芥(Arabidopsis halleri)
<400> 43
Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Leu Arg Asp Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Met Thr Pro Gln Ile Glu Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala Ile Pro Thr Ala
50 55 60
Val Gly Ile Arg Asn Val Leu Arg His Ile Gly Ala His Lys Asp Gly
65 70 75 80
Lys Gln Ala Lys Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Lys Pro Phe
100 105 110
Thr Asn Leu Glu Tyr Thr Gly Ile Asn Arg Val Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ser Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Pro Val Ser Thr Asp Ser Leu Lys Thr Leu Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Ala Glu Gly Tyr Leu Leu Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Thr Asp Phe Asp Leu Leu
195 200 205
Ile Arg Lys Ile Ser Gln Ala Asp Ile Asn Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Phe Lys Arg Thr Gly Ala Ser Asp Gln Gly Phe Pro Arg
245 250 255
Asn Asn Ser Phe Gly Arg Ile Phe Lys Ala Gly Glu Asn Ile Thr Val
260 265 270
Asn Leu Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val
275 280 285
Val Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg
290 295 300
Gln Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg
305 310 315 320
Glu Ala Ile Ala Thr Tyr Arg Ser Ser Ile Leu Arg Gln Pro Asp Glu
325 330 335
Lys Lys Arg Glu Ala Ala Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg
340 345 350
Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Leu His Ser Glu Gly Ala
355 360 365
Phe Leu Gln Ser Gly Ser Leu Gly His Val Ser Phe Ala Asp Trp Met
370 375 380
Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg
385 390 395 400
Asp Pro Met Gly Ala Leu Gly Tyr Ala Ala Met Lys Pro Ser
405 410
<210> 44
<211> 408
<212> PRT
<213> 黄瓜(Cucumis sativus)
<400> 44
Met Ser Leu Thr Pro Lys Glu Pro Glu His Val Met Lys Phe Arg Gly
1 5 10 15
Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Asn Ser Leu Gln Val Tyr Gly Val Ala Ile Pro Thr
50 55 60
Val Leu Gly Ile Gln Asn Val Leu Lys His Val Gly Ala Gln Lys Asp
65 70 75 80
Gly Gln Lys Ala Gln Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val
85 90 95
Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro
100 105 110
Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asp Arg Ala Arg Val Glu Gln
115 120 125
Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Ile Glu Ala Ser Arg Tyr
130 135 140
Gly Asn Lys Ile Leu Val Thr Asp Glu Met Pro Asp Gly Gln Met Val
145 150 155 160
Asp Gln Trp Glu Leu Val Ser His Asp Ser Ile Lys Thr Pro Leu Glu
165 170 175
Val Tyr Glu Glu Leu Gln Val Glu Gly Val His Val Asp Tyr Glu Arg
180 185 190
Val Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Ser Asp Phe Asp Ile
195 200 205
Leu Val Arg Lys Ile Ser Gln Val Asp Ile Asn Thr Ala Ile Ile Phe
210 215 220
Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala
225 230 235 240
Thr Leu Val Tyr Leu Asn Arg Val Gly Ala Ser Gly Ile Ala Arg Thr
245 250 255
Asn Ser Ile Gly Lys Val Ser Asp Cys Ser Ala Asn Val Asp Asp Asn
260 265 270
Leu Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile
275 280 285
Arg Ser Leu Thr Arg Val Leu Glu Gly Gly Ala Glu Gly Lys Arg Gln
290 295 300
Val Asp Glu Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu
305 310 315 320
Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Ala Asp Glu Met
325 330 335
Lys Arg Glu Ala Ala Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr
340 345 350
Tyr Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ser Gly
355 360 365
Leu Arg Ser Thr Ser Ser Ser His Cys Ser Phe Ala Glu Trp Met Lys
370 375 380
Ala Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp
385 390 395 400
Pro Met Gly Ala Leu Gly Tyr Ala
405
<210> 45
<211> 415
<212> PRT
<213> 白菜型油菜(Brassica rapa)
<400> 45
Met Ala Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Leu Arg Asp
1 5 10 15
Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Met Thr Pro Gln Ile Asp Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Glu Ser Leu Arg Val His Gly Val Ala Ile Pro Thr
50 55 60
Ala Val Gly Ile Arg Asn Val Leu Arg His Ile Gly Ala His Lys Asp
65 70 75 80
Gly Lys Gln Val Gln Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val
85 90 95
Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Lys Pro
100 105 110
Phe Thr Asn Leu Glu Tyr Thr Gly Ile Asn Arg Val Arg Val Glu Gln
115 120 125
Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ser Arg Tyr
130 135 140
Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val
145 150 155 160
Asp Gln Trp Glu Pro Val Ser Thr Asp Ser Leu Lys Thr Leu Leu Glu
165 170 175
Val Tyr Glu Glu Leu Gln Ala Glu Gly Tyr Leu Val Asp Tyr Glu Arg
180 185 190
Val Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Thr Asp Phe Asp Ala
195 200 205
Leu Ile Lys Lys Ile Ser Gln Ala Asp Ile Asn Thr Glu Ile Ile Phe
210 215 220
Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala
225 230 235 240
Thr Leu Val Tyr Phe Lys Arg Thr Gly Ala Ser Asp His Gly Phe Pro
245 250 255
Arg Asn Asn Ser Phe Gly Arg Ile Phe Lys Ala Gly Glu Asn Ile Thr
260 265 270
Ile Asn Leu Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala
275 280 285
Val Val Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys
290 295 300
Arg Gln Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu
305 310 315 320
Arg Glu Ala Ile Ala Thr Tyr Arg Ser Ser Ile Leu Arg Gln Pro Asp
325 330 335
Glu Lys Lys Arg Glu Ala Ala Leu Ser Phe Phe Val Glu Tyr Leu Glu
340 345 350
Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Leu His Ser Glu Gly
355 360 365
Gly Tyr Leu Gln Ser Gly Ser Leu Gly His Val Ser Phe Ser Asp Trp
370 375 380
Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg
385 390 395 400
Arg Asp Pro Met Gly Ala Leu Gly Tyr Ala Ala Met Lys Pro Ser
405 410 415
<210> 46
<211> 412
<212> PRT
<213> 木薯(Manihot esculenta)
<400> 46
Met Ser Leu Pro Lys Glu Leu Glu Gln Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Thr Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Pro Val His Gly Val Ala Ile Pro Thr Thr
50 55 60
Glu Gly Ile Arg Asn Val Leu Lys His Ile Gly Ala Gln Lys Asp Gly
65 70 75 80
Lys Arg Ala Gln Val Leu Trp Phe Asn Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Val Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Ser Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Leu Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Pro Val Ser Arg Asp Ser Val Lys Thr Pro Leu Glu Ala
165 170 175
Asn Glu Glu Leu Gln Leu Glu Gly Tyr Leu Phe Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ser Pro Glu Glu Gln Asp Phe Asp Ile Leu
195 200 205
Val Asp Lys Ile Tyr Arg Ala Asn Leu Asn Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser Asn
245 250 255
Ser Ile Gly Arg Val Phe Asp Ala Gly Ser Thr Val Ala Asp Asn Leu
260 265 270
Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg
275 280 285
Ser Leu Thr Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Asn Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg Asp Ala Leu
355 360 365
Arg Ser Ser Ser Phe Val His Ser Ser Phe Ala Asp Trp Met Arg Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
405 410
<210> 47
<211> 412
<212> PRT
<213> 甜菜(Beta vulgaris)
<400> 47
Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Ser Ile Glu Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Gln Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Asp Gly Ile Arg Asn Val Leu Lys His Ile Gly Ala Arg Ala Asp Ile
65 70 75 80
Lys Arg Thr His Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Asp Arg Val Glu Asp Met
115 120 125
Glu Ser Arg Leu Lys Glu Asp Ile Leu Leu Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Thr Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Pro Met Thr Cys Asp Phe Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Lys Met Glu Gly Phe Leu Val Asn Tyr Glu Arg Val
180 185 190
Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Ser Asp Phe Asp Met Leu
195 200 205
Val Asn Lys Ile Thr Leu Val Asp Leu Asn Thr Ala Val Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Ile Tyr Leu Asn Arg Ile Gly Ala Ser Ala Gly Ile Pro Arg Ser
245 250 255
Asn Ser Ile Gly Arg Val Ser Asp Leu Ser Cys Gly Ala Asp Asn Leu
260 265 270
Leu Asn Ser Glu Asp Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu His Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Leu His Thr Glu Glu Thr Ala Val
355 360 365
Asn Pro Ser Phe Pro Ala Gln Cys Ser Phe Ser Asp Trp Met Arg Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Arg Asn Ala Glu Lys Ser
405 410
<210> 48
<211> 414
<212> PRT
<213> 琴叶拟南芥(Arabidopsis lyrata)
<400> 48
Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Leu Arg Asp Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Met Thr Pro Gln Ile Glu Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala Ile Pro Thr Ala
50 55 60
Val Gly Ile Arg Asn Val Leu Arg His Ile Gly Ala His Lys Asp Gly
65 70 75 80
Lys Gln Ala Lys Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Lys Pro Phe
100 105 110
Thr Asn Leu Glu Tyr Thr Gly Ile Asn Arg Val Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ser Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Pro Val Ser Thr Asp Ser Leu Lys Thr Leu Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Ala Glu Gly Tyr Leu Leu Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Thr Asp Phe Asp Leu Leu
195 200 205
Ile Arg Lys Ile Ser Gln Ala Asp Ile Asn Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Phe Lys Arg Thr Arg Ala Ser Asp Gln Gly Phe Pro Arg
245 250 255
Asn Asn Ser Phe Gly Arg Ile Phe Lys Ala Gly Glu Asn Ile Thr Val
260 265 270
Asn Leu Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val
275 280 285
Val Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg
290 295 300
Gln Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg
305 310 315 320
Glu Ala Ile Ala Thr Tyr Arg Ser Ser Ile Leu Arg Gln Pro Asp Glu
325 330 335
Lys Lys Arg Glu Ala Ala Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg
340 345 350
Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Leu His Ser Glu Gly Ala
355 360 365
Phe Leu Gln Ser Gly Ser Leu Gly His Val Ser Phe Ala Asp Trp Met
370 375 380
Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg Leu Leu Arg Arg
385 390 395 400
Asp Pro Met Gly Ala Leu Gly Tyr Ala Ala Met Lys Pro Ser
405 410
<210> 49
<211> 418
<212> PRT
<213> 花椰菜(Brassica oleracea)
<400> 49
Met Arg Glu Met Ala Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys
1 5 10 15
Leu Arg Asp Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp
20 25 30
His Phe Pro Gly Cys Gln Asn Lys Arg Met Thr Pro Gln Ile Asp Gly
35 40 45
Ala Pro Asn Tyr Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala
50 55 60
Ile Pro Thr Ala Val Gly Ile Arg Asn Val Leu Arg His Ile Gly Ala
65 70 75 80
His Lys Asp Gly Lys Gln Val Gln Val Leu Trp Ile Ser Leu Arg Glu
85 90 95
Glu Pro Val Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val
100 105 110
Glu Lys Pro Tyr Thr Asn Leu Glu Tyr Thr Gly Ile Asn Arg Val Arg
115 120 125
Val Glu Gln Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala
130 135 140
Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly
145 150 155 160
Gln Met Val Asp Gln Trp Glu Pro Val Ser Thr Asp Ser Leu Lys Thr
165 170 175
Leu Leu Glu Phe Tyr Glu Glu Leu Gln Ala Glu Gly Tyr Leu Val Asp
180 185 190
Tyr Glu Arg Val Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Thr Asp
195 200 205
Phe Asp Ala Leu Ile Lys Lys Ile Ser Gln Ala Asp Ile Asn Thr Glu
210 215 220
Ile Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met
225 230 235 240
Val Ile Ala Thr Leu Val Tyr Phe Lys Arg Thr Gly Ala Ser Asp His
245 250 255
Gly Phe Pro Arg Asn Asn Ser Phe Gly Arg Ile Phe Lys Ala Gly Glu
260 265 270
Asn Ile Thr Ile Asn Leu Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly
275 280 285
Glu Tyr Ala Val Val Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val
290 295 300
Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met
305 310 315 320
Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Ser Ser Ile Leu Arg
325 330 335
Gln Pro Asp Glu Lys Lys Arg Glu Ala Ala Leu Ser Phe Phe Val Glu
340 345 350
Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Leu His
355 360 365
Ser Glu Gly Gly Tyr Leu Gln Ser Gly Ser Leu Gly His Val Ser Phe
370 375 380
Ser Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg
385 390 395 400
Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ala Ala Met Lys
405 410 415
Pro Ser
<210> 50
<211> 411
<212> PRT
<213> 猕猴桃(Actinidia chinensis Red5)
<400> 50
Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Gln Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu His Val His Gly Val Ala Ile Pro Thr Thr
50 55 60
Asp Gly Ile Arg Asn Val Leu Asn His Ile Gly Ala Gln Leu Asp Glu
65 70 75 80
Lys Gln Thr Arg Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Asp Arg Val Glu Gln Met
115 120 125
Glu Asp Arg Leu Lys Glu Asp Ile Leu Leu Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Val Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Pro Val Thr Asn Val Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Ala Lys Gln Tyr Leu Val Asp Tyr Glu Arg Val
180 185 190
Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Gln Asp Phe Asp Ile Leu
195 200 205
Val Asp Lys Ile Ser Gln Val Asp Ile Asn Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Asn
245 250 255
Ser Ile Gly Lys Val Ser Asn Ser Gly Ser Ser Val Thr Asp Asn Leu
260 265 270
Pro Asn Ser Glu Asp Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Leu His Thr Glu Arg Ala Leu His
355 360 365
Pro Lys Ser Pro Asp Gln Arg Ser Phe Ala Asp Trp Met Lys Ala Arg
370 375 380
Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asn Pro Met
385 390 395 400
Gly Ala Leu Gly Tyr Ala Asn Ser Asn Pro Val
405 410
<210> 51
<211> 413
<212> PRT
<213> 桃(Prunus persica)
<400> 51
Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu His Val His Gly Val Ala Ile Pro Thr Val
50 55 60
Asp Gly Ile Gln Asn Val Leu Asn His Ile Gly Ala Gln Gln Ile Asp
65 70 75 80
Gly Lys Arg Thr Gln Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val
85 90 95
Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro
100 105 110
Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Ala Arg Leu Glu Gln
115 120 125
Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Ile Glu Ala Ala Arg Tyr
130 135 140
Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val
145 150 155 160
Asp Gln Trp Glu Pro Val Ser Arg Asp Ser Val Thr Thr Pro Leu Glu
165 170 175
Val Tyr Glu Glu Leu Gln Val Gln Gly Tyr Leu Val Asp Tyr Glu Arg
180 185 190
Val Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile
195 200 205
Leu Val His Lys Ile Ser Gln Ala Asp Ile Asn Ala Glu Ile Ile Phe
210 215 220
Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala
225 230 235 240
Thr Leu Ile Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr
245 250 255
Asn Ser Ile Gly Lys Val Ser Asp Ser Ser Ala Ile Val Thr Asp Asn
260 265 270
Phe Pro Asn Ser Glu Asp Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile
275 280 285
Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln
290 295 300
Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu
305 310 315 320
Ala Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met
325 330 335
Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr
340 345 350
Tyr Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala
355 360 365
Leu Arg Ser Ser Ser Val Gly Tyr Ser Ser Phe Ala Asp Trp Met Lys
370 375 380
Ala Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp
385 390 395 400
Pro Met Gly Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
405 410
<210> 52
<211> 418
<212> PRT
<213> 欧洲油菜(Brassica napus)
<400> 52
Met Arg Glu Met Ala Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys
1 5 10 15
Leu Arg Asp Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp
20 25 30
His Phe Pro Gly Cys Gln Asn Lys Arg Met Thr Pro Gln Ile Asp Gly
35 40 45
Ala Pro Asn Tyr Arg Gln Ala Glu Ser Leu Arg Val His Gly Val Ala
50 55 60
Ile Pro Thr Ala Val Gly Ile Arg Asn Val Leu Arg His Ile Gly Ala
65 70 75 80
His Lys Asp Gly Lys Gln Val Gln Val Leu Trp Ile Ser Leu Arg Glu
85 90 95
Glu Pro Val Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val
100 105 110
Glu Lys Pro Phe Thr Asn Leu Glu Tyr Thr Gly Ile Asn Arg Val Arg
115 120 125
Val Glu Gln Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala
130 135 140
Ser Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly
145 150 155 160
Gln Met Val Asp Gln Trp Glu Pro Val Ser Thr Asp Ser Leu Lys Thr
165 170 175
Leu Ile Glu Val Tyr Glu Glu Leu Gln Ala Glu Gly Tyr Leu Val Asp
180 185 190
Tyr Glu Arg Val Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Thr Asp
195 200 205
Phe Asp Ala Leu Ile Lys Lys Ile Ser Gln Ala Asp Ile Asn Thr Glu
210 215 220
Ile Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met
225 230 235 240
Val Ile Ala Thr Leu Val Tyr Phe Lys Arg Thr Gly Ala Ser Asp His
245 250 255
Gly Phe Pro Arg Asn Asn Ser Phe Gly Arg Ile Phe Lys Ala Gly Glu
260 265 270
Asn Ile Thr Ile Asn Leu Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly
275 280 285
Glu Tyr Ala Val Val Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val
290 295 300
Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met
305 310 315 320
Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr Arg Ser Ser Ile Leu Arg
325 330 335
Gln Pro Asp Glu Lys Lys Arg Glu Ala Ala Leu Ser Phe Phe Val Glu
340 345 350
Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Leu His
355 360 365
Ser Glu Gly Gly Tyr Leu Gln Ser Gly Ser Leu Gly His Val Ser Phe
370 375 380
Ser Asp Trp Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Leu Arg Arg
385 390 395 400
Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr Ala Ala Met Lys
405 410 415
Pro Ser
<210> 53
<211> 410
<212> PRT
<213> 胡萝卜(Daucus carota)
<400> 53
Met Ser Glu Glu Pro Glu Leu Val Ile Lys Gln Arg Asp Gly Ser Val
1 5 10 15
Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly Cys His
20 25 30
Asn Lys Arg Leu Ser Pro His Ile Asp Gly Ala Pro Asn Phe Arg Gln
35 40 45
Ala Glu Ser Leu His Val Tyr Gly Val Ala Ile Pro Thr Thr Ile Gly
50 55 60
Ile Gln Asn Leu Leu Asp His Ile Gly Ala His Ile Ser Gly Lys Lys
65 70 75 80
Thr Lys Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val Val Tyr Ile
85 90 95
Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn
100 105 110
Leu Glu His Thr Gly Ile Asn Arg Leu Arg Leu Glu Gln Met Glu Asp
115 120 125
Arg Leu Arg Glu Asp Ile Leu Gln Glu Ala Ser Arg Tyr Gly Asn Lys
130 135 140
Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp
145 150 155 160
Glu Pro Val Thr Gln Glu Ser Val Ile Thr Pro Leu Glu Val Tyr Leu
165 170 175
Glu Leu Gln Asn Arg Ser Tyr Leu Val Asp Tyr Asp Arg Val Pro Ile
180 185 190
Thr Asp Glu Lys Ser Pro Lys Glu Gln Asp Phe Asp Ile Leu Val His
195 200 205
Arg Ile Leu Gln Ala Asp Val Glu Thr Arg Ile Ile Phe Asn Cys Gln
210 215 220
Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Ile
225 230 235 240
Tyr Phe Asn Arg Ile Gly Ser Phe Gly Ile Leu Arg Thr Ser Ser Leu
245 250 255
Gly Thr Ile Ser Asp Ser Ala Ser Ser Ala Ala Asp Asn Met Pro Asn
260 265 270
Ser Glu Glu Ala Val Ser Arg Gly Glu Tyr Thr Val Ile Arg Ser Leu
275 280 285
Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Leu Val Asp Lys
290 295 300
Val Ile Asp Lys Cys Ser Ser Met Gln Asn Leu Arg Glu Ser Ile Ala
305 310 315 320
Thr Tyr Arg Asn Ser Val Leu Arg Gln Ser Asp Glu Met Lys Arg Ala
325 330 335
Ala His Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Tyr Leu
340 345 350
Ile Cys Phe Ser Val Tyr Leu His Thr Glu Arg Val Ala Ile Asn Ser
355 360 365
Gly Ser Ser Asn His Cys Ser Phe Tyr Asp Trp Met Lys Ser Arg Pro
370 375 380
Glu Leu Tyr Thr Ile Ile Gln Arg Leu Leu Arg Arg Asp Pro Met Gly
385 390 395 400
Ala Leu Gly Tyr Ala Arg Thr Lys Pro Thr
405 410
<210> 54
<211> 383
<212> PRT
<213> 胡萝卜(Daucus carota)
<400> 54
Met Ser Lys Lys Pro Thr Ser Pro Phe Ala Lys His Pro Leu Tyr Ile
1 5 10 15
Phe Val Ile Asn Leu Ala Glu Ser Phe His Val Tyr Gly Val Ala Ile
20 25 30
Pro Thr Thr Asp Gly Ile Arg Asn Leu Leu Asp His Ile Gly Ala Gln
35 40 45
Arg Ile Gly Lys Gln Thr Gln Val Leu Trp Ile Asn Leu Arg Glu Glu
50 55 60
Pro Val Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
65 70 75 80
Arg Pro Phe Ser Asn Leu Glu His Thr Gly Ile Asn Arg Phe Arg Leu
85 90 95
Glu Gln Met Glu Asp Arg Leu Arg Glu Asp Ile Leu Gln Glu Ala Ser
100 105 110
Arg Tyr Gly Asn Met Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln
115 120 125
Met Val Asp Gln Trp Glu Pro Val Thr Gln Glu Ser Val Lys Thr Pro
130 135 140
Leu Glu Val Tyr Leu Glu Leu Gln Asn Arg Ser Tyr Met Val Asp Tyr
145 150 155 160
Glu Arg Val Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Gln Asp Phe
165 170 175
Asp Thr Leu Val His Arg Ile Ser Gln Ala Asp Thr Glu Thr Gln Ile
180 185 190
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
195 200 205
Ile Ala Thr Leu Ile Tyr Leu His Arg Ile Gly Ser Ser Gly Ile Thr
210 215 220
Arg Thr Ser Ser Leu Gly Lys Ile Ser Asp Tyr Cys Ser Asn Val Ala
225 230 235 240
Asp Lys Met Pro Asn Ser Glu Glu Ala Val Ser Arg Gly Glu Tyr Thr
245 250 255
Val Ile Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys
260 265 270
Arg Gln Val Asp Lys Val Ile Asp Gln Cys Ser Ser Met Gln Asn Leu
275 280 285
Arg Glu Ala Ile Thr Thr Tyr Arg Asn Ser Ile Leu Arg Gln Ser Asp
290 295 300
Glu Met Lys Arg Ala Ala Leu Leu Ser Phe Phe Val Glu Tyr Leu Glu
305 310 315 320
Arg Tyr Tyr Tyr Leu Ile Cys Phe Ser Val Tyr Leu His Thr Glu Arg
325 330 335
Ala Ala Leu Glu Pro Gly Ser Ser Ser His Cys Ser Phe Tyr Glu Trp
340 345 350
Met Lys Ser Arg Pro Glu Leu Tyr Thr Ile Ile Gln Arg Leu Leu Arg
355 360 365
Arg Asp Pro Met Gly Ala Leu Gly Tyr Ala Lys Thr Lys Thr Ser
370 375 380
<210> 55
<211> 328
<212> PRT
<213> 绿豆(Vigna radiata)
<400> 55
Met Ser Gln Ile Leu Lys Glu Pro Glu Gln Leu Ala Tyr Ile Asn Gly
1 5 10 15
Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn Leu Glu
20 25 30
Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu Ala Arg Leu
35 40 45
Lys Glu Asp Ile Leu Val Glu Ala Ala Arg Tyr Gly Asn Lys Ile Leu
50 55 60
Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp Glu Pro
65 70 75 80
Val Ser Cys Asp Ser Val Lys Thr Pro Leu Glu Val Tyr Glu Glu Leu
85 90 95
Gln Val Ala Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr Asp
100 105 110
Glu Lys Ser Pro Lys Glu Met Asp Phe Asp Ile Leu Val Asn Lys Ile
115 120 125
Ser Gln Ala Asp Val Asp Thr Glu Ile Ile Phe Asn Cys Gln Met Gly
130 135 140
Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr Leu
145 150 155 160
Asn Arg Ile Gly Ala Ser Gly Phe Pro Arg Ser Asn Ser Ile Gly Arg
165 170 175
Ile Phe Gln Ser Met Thr Asn Val Ala Asp His Leu Pro Asp Thr Glu
180 185 190
Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Ile Arg
195 200 205
Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile
210 215 220
Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr Tyr
225 230 235 240
Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ser
245 250 255
Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys
260 265 270
Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu Arg Ser Asn Thr
275 280 285
Thr Asp Asn Cys Ser Phe Ala Asp Trp Met Arg Ala Arg Pro Glu Leu
290 295 300
Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu
305 310 315 320
Gly Tyr Ser Ser Leu Lys Pro Ser
325
<210> 56
<211> 405
<212> PRT
<213> 向日葵(Helianthus annuus)
<400> 56
Met Ser Val Pro Lys Glu Pro Ala Gln Val Met Lys Gln Arg Asp Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Glu Ser Met Arg Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Asp Gly Ile Arg Asn Val Leu Thr Tyr Leu Gly Ala Gln Met Glu Gly
65 70 75 80
Lys Gln Ala Asn Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Glu Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Ala Arg Val Glu Gln Met
115 120 125
Glu Asp Arg Leu Lys Glu Asp Ile Leu Leu Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Ser Val Thr Pro Glu Ser Val Lys Thr Pro Leu Gln Val
165 170 175
Tyr Met Glu Leu Gln Thr Lys Gln Phe Leu Val Asp Tyr Glu Arg Ile
180 185 190
Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Gln Asp Phe Asp Thr Leu
195 200 205
Val Asp Arg Ile Ser Arg Ala Asp Leu Lys Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Ile Tyr Phe Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Asn
245 250 255
Ser Ile Gly Lys Val Ser Val Tyr Gly Ser Asn Ala Ser Asp Asn Met
260 265 270
Leu Asn Thr Glu Glu Ala Leu Leu Arg Gly Glu Tyr Thr Val Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ser Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Ala Ala Leu Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Tyr Leu Ile Cys Phe Ala Val Tyr Leu His Thr Glu Lys Ser Ser Ser
355 360 365
Asn Phe Thr Phe Tyr His Trp Met Lys Ala Arg Pro Glu Leu Tyr Thr
370 375 380
Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly Tyr
385 390 395 400
Ala Asn Ile Lys Ser
405
<210> 57
<211> 409
<212> PRT
<213> 红豆(Vigna angularis)
<400> 57
Met Ser Gln Ile Leu Lys Glu Pro Glu Gln Val Met Lys Met Arg Gly
1 5 10 15
Gly Gly Val Leu Gly Lys Arg Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Glu Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Ser Asp Ser Leu His Val His Gly Val Ala Ile Pro
50 55 60
Thr Ile Asp Gly Ile Arg Asn Val Leu Asn His Ile Gly Ala Gly Leu
65 70 75 80
Lys Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Leu Ala Tyr Ile Asn
85 90 95
Gly Arg Pro Phe Val Leu Arg Asp Val Glu Lys Pro Phe Ser Asn Leu
100 105 110
Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu Ala Arg
115 120 125
Leu Lys Glu Asp Ile Leu Val Glu Ala Ala Arg Tyr Gly Asn Lys Ile
130 135 140
Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp Glu
145 150 155 160
Pro Val Ser Cys Asp Ser Val Lys Thr Pro Leu Glu Val Tyr Glu Glu
165 170 175
Leu Gln Val Ala Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr
180 185 190
Asp Glu Lys Ser Pro Lys Glu Met Asp Phe Asp Ile Leu Val Asn Lys
195 200 205
Ile Ser Gln Ala Asp Val Asp Thr Glu Ile Ile Phe Asn Cys Gln Met
210 215 220
Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr
225 230 235 240
Leu Asn Arg Ile Gly Ala Ser Gly Phe Pro Arg Ser Asn Ser Ile Gly
245 250 255
Arg Ile Phe Gln Ser Met Thr Asn Val Ala Asp His Leu Pro Asp Thr
260 265 270
Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Ile
275 280 285
Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val
290 295 300
Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr
305 310 315 320
Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala
325 330 335
Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile
340 345 350
Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu Arg Ser Asn
355 360 365
Thr Thr Asp Asn Cys Ser Phe Ala Asp Trp Met Arg Ala Arg Pro Glu
370 375 380
Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala
385 390 395 400
Leu Gly Tyr Ser Ser Leu Lys Pro Ser
405
<210> 58
<211> 412
<212> PRT
<213> 黄麻(Corchorus capsularis)
<400> 58
Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Met Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Lys Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Val Gly Ile Arg Asn Val Leu Lys His Ile Gly Ala Gln Lys Asp Gly
65 70 75 80
Lys Gln Ala Arg Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Asp Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Arg Val Ser Thr Asp Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Leu Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Pro Pro Lys Glu Leu Asp Phe Asp Ile Leu
195 200 205
Val Asn Lys Ile Ser Gln Ala Asp Ile Ser Thr Glu Val Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Asn
245 250 255
Ser Val Gly Arg Val Phe Glu Ser Gly Ser Asn Val Thr Asp Asn Leu
260 265 270
Pro Asp Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Val Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Asp Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu
355 360 365
Arg Ala Ser Ser Cys Gly His Thr Ser Phe Ala Asp Trp Met Lys Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
405 410
<210> 59
<211> 409
<212> PRT
<213> 四季豆(Phaseolus vulgaris)
<400> 59
Met Ser Gln Ile Pro Lys Glu Pro Glu Gln Val Met Lys Met Arg Gly
1 5 10 15
Gly Gly Val Leu Gly Lys Arg Thr Ile Leu Lys Ser Asp His Phe Pro
20 25 30
Gly Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Glu Gly Ala Pro Asn
35 40 45
Tyr Arg Gln Ala Ser Asp Ser Leu His Val His Gly Val Ala Ile Pro
50 55 60
Thr Ile Asp Gly Ile Arg Asn Val Leu Asp His Ile Gly Ala Arg Leu
65 70 75 80
Lys Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Leu Ala Tyr Ile Asn
85 90 95
Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe Ser Asn Leu
100 105 110
Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met Glu Ala Arg
115 120 125
Leu Lys Glu Asp Ile Leu Val Glu Ala Ala Arg Tyr Gly Asn Lys Ile
130 135 140
Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp Glu
145 150 155 160
Pro Val Ser Cys Asn Ser Val Lys Thr Pro Leu Glu Val Tyr Glu Glu
165 170 175
Leu Gln Val Ala Gly Tyr Leu Val Asp Tyr Glu Arg Val Pro Ile Thr
180 185 190
Asp Glu Lys Ser Pro Lys Glu Met Asp Phe Asp Ile Leu Val Asn Lys
195 200 205
Ile Ser Gln Ala Asp Val Asp Thr Glu Ile Ile Phe Asn Cys Gln Met
210 215 220
Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr
225 230 235 240
Leu Asn Arg Ile Gly Ala Ser Gly Phe Pro Arg Ser Asn Ser Ile Gly
245 250 255
Arg Ile Phe Gln Ser Met Thr Asn Gly Ala Asp His Leu Pro Asp Ser
260 265 270
Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu Ile
275 280 285
Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val
290 295 300
Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Thr
305 310 315 320
Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala
325 330 335
Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile
340 345 350
Cys Phe Ala Val Tyr Ile His Ser Glu Arg Ala Ala Leu Arg Ser Asn
355 360 365
Thr Ala Asp Asn Cys Ser Phe Ala Asp Trp Met Arg Ala Arg Pro Glu
370 375 380
Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala
385 390 395 400
Leu Gly Tyr Ser Ser Leu Lys Pro Ser
405
<210> 60
<211> 412
<212> PRT
<213> 红三叶草(Trifolium pratense)
<400> 60
Met Ser Ile Pro Lys Glu Pro Glu Glu Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Arg Pro Asn Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu His Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Asp Gly Ile Arg Asn Val Leu Lys His Ile Gly Ala Gln Thr Glu Gly
65 70 75 80
Lys Lys Val His Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Glu Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Asn Glu Ala Ala Arg Tyr Gly
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Ser Val Ser Cys Asn Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Val Glu Gly Tyr Phe Val Asp Tyr Glu Arg Val
180 185 190
Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Gln Asp Phe Asp Ile Leu
195 200 205
Val His Lys Ile Ser Gln Ala Asp Val Asn Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Ile Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Asn Asn
245 250 255
Ser Val Gly Thr Ile Ser Gln Cys Leu Thr Asn Val Pro Asp His Met
260 265 270
Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Lys Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Met Ala Ala Leu
355 360 365
Gln Ala Met Ser Ala Ser His Ser Ser Phe Ala Asp Trp Met Arg Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ser Ser Leu Lys Pro Ser
405 410
<210> 61
<211> 415
<212> PRT
<213> 毛果杨(Populus trichocarpa)
<400> 61
Met Ser Met Ala Val Val Gln Lys Glu Pro Glu Lys Val Met Lys Leu
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Glu Ser Leu Pro Val His Gly Val Ala Ile
50 55 60
Pro Thr Ile Glu Gly Cys Arg Asn Val Ile Lys His Ile Arg Gly Arg
65 70 75 80
Lys Asp Gly Lys Gln Ala Gln Val Leu Trp Phe Asn Leu Arg Glu Glu
85 90 95
Pro Leu Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Ser Arg Val
115 120 125
Glu Glu Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala
130 135 140
Arg Tyr Gly Asn Lys Ile His Val Thr Asp Glu Leu Pro Asp Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Pro Val Ser Cys Asp Ser Val Lys Thr Pro
165 170 175
Val Glu Val Tyr Glu Asp Leu Gln Val Glu Gly His Leu Tyr Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ser Pro Glu Glu Gln Asp Phe
195 200 205
Asp Ile Leu Val Asp Arg Ile Tyr Gln Thr Asp Leu Asn Thr Asp Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Thr Thr Leu Val Tyr Leu Asn Arg Ile Gly Asp Ser Gly Ile Gln
245 250 255
Arg Thr Asn Ser Val Gly Arg Ile Cys Glu Phe Gly Leu Asn Val Asn
260 265 270
Glu Asn Leu Pro Asn Ser Glu Glu Ala Leu Leu Arg Gly Glu Tyr Ala
275 280 285
Val Ile Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys
290 295 300
Lys Gln Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu
305 310 315 320
Arg Glu Ala Ile Ala Asn Tyr Arg Asn Ser Ile Leu Arg Gln Ser Asp
325 330 335
Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu
340 345 350
Arg Tyr Tyr Ser Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg
355 360 365
Asp Ala Leu Arg Ser Ser Ser Phe Gly His Ser Ser Phe Ala Asp Trp
370 375 380
Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg
385 390 395 400
Arg Asn Pro Met Gly Ala Leu Gly Tyr Ala Ser Pro Lys Pro Ser
405 410 415
<210> 62
<211> 415
<212> PRT
<213> 毛果杨(Populus trichocarpa)
<400> 62
Met Ser Asn Ala Val Val Glu Lys Glu Pro Glu Gln Val Met Lys Leu
1 5 10 15
Arg Gly Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Thr Pro Gln Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Gln Ala Asp Ser Leu Pro Val His Gly Val Ala Ile
50 55 60
Pro Thr Ile Glu Gly Cys Arg Asn Val Ile Lys His Ile Arg Gly Arg
65 70 75 80
Lys Asp Gly Lys Gln Ala Gln Val Leu Trp Phe Asn Leu Arg Glu Glu
85 90 95
Pro Leu Val Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Ser Arg Val
115 120 125
Glu Glu Met Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Pro Val Ser Cys Asp Ser Val Lys Thr Pro
165 170 175
Leu Glu Val Tyr Glu Asp Leu Gln Glu Glu Gly Tyr Leu Tyr Asp Tyr
180 185 190
Glu Arg Val Pro Val Thr Asp Glu Lys Ser Pro Glu Glu Gln Asp Phe
195 200 205
Asp Thr Leu Val Asp Arg Ile Tyr Gln Thr Asp Leu Asn Ala Asp Ile
210 215 220
Ile Phe Asn Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val
225 230 235 240
Ile Ala Thr Leu Val Phe Leu Asn Arg Ile Gly Asp Ser Gly Ile Gln
245 250 255
Arg Thr Asn Ser Val Gly Arg Ile Phe Asp Phe Gly Leu Asn Val Asn
260 265 270
Glu Asn Leu Pro Asn Ser Glu Asp Ala Leu Arg Arg Gly Glu Tyr Ala
275 280 285
Val Val Arg Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys
290 295 300
Lys Gln Val Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu
305 310 315 320
Arg Glu Ala Ile Ala Asn Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp
325 330 335
Glu Met Lys Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu
340 345 350
Arg Tyr Tyr Phe Leu Ile Cys Phe Ala Val Tyr Ile His Ser Glu Arg
355 360 365
Val Ala Leu Arg Ser Ser Ser Phe Val His Ser Ser Phe Ala Asp Trp
370 375 380
Met Arg Ala Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg
385 390 395 400
Arg Asp Pro Met Gly Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
405 410 415
<210> 63
<211> 412
<212> PRT
<213> 可可(Theobroma cacao)
<400> 63
Met Ser Leu Pro Lys Glu Pro Glu Gln Val Met Lys Met Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Leu Arg Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Val Gly Ile Gln Asn Val Leu Lys His Ile Gly Ala Gln Lys Asp Gly
65 70 75 80
Lys Gln Ala His Val Leu Trp Ile Ser Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg His Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr Ala
130 135 140
Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Arg Val Ser Phe Asp Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Leu Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val
180 185 190
Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu
195 200 205
Val Asn Lys Ile Ser Gln Ala Asp Ile Ser Thr Glu Val Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Thr Asn
245 250 255
Ser Ile Gly Arg Val Phe Glu Ser Gly Ser Asn Val Thr Asp Ser Met
260 265 270
Pro Asn Ser Glu Val Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ser Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Ala Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys
325 330 335
Arg Glu Ala Ser Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Phe His Ser Glu Arg Ala Ala Leu
355 360 365
Arg Ser Ser Ser Cys Asp His Thr Ser Phe Ala Asp Trp Met Lys Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
405 410
<210> 64
<211> 186
<212> PRT
<213> 烟草(Nicotiana attenuata)
<400> 64
Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ser Thr Leu Val
1 5 10 15
Tyr Leu Asn Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser Asn Ser Ile
20 25 30
Gly Arg Val Ser Asp Cys Val Ser Asn Leu Asn Asp Thr Leu Pro Asn
35 40 45
Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Val Ile Arg Ser Leu
50 55 60
Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys
65 70 75 80
Val Ile Asp Lys Cys Ser Ser Met Gln Asn Leu Arg Glu Ala Ile Ala
85 90 95
Ile Tyr Arg Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu
100 105 110
Ala Ala Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu
115 120 125
Ile Cys Phe Ala Val Tyr Leu His Thr Gln Arg Asp Ala Leu Phe Ser
130 135 140
Arg Ser Ser Ala His Cys Ser Phe Ser Asp Trp Met Lys Ala Arg Pro
145 150 155 160
Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly
165 170 175
Ala Leu Gly Tyr Ala Ser Leu Lys Pro Ser
180 185
<210> 65
<211> 215
<212> PRT
<213> 烟草(Nicotiana attenuata)
<400> 65
Met Arg Ser Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Gln
1 5 10 15
Arg Asp Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Arg Arg Leu Ser Pro His Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Lys Ala Gly Ser Ser His Val His Gly Val Ala Ile
50 55 60
Pro Thr Val Glu Gly Ile Gln Asn Val Leu Asp His Ile Gly Ala Gln
65 70 75 80
Leu Ser Gly Lys Lys Thr His Phe Leu Trp Ile Asn Leu Arg Glu Glu
85 90 95
Pro Val Ile Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Glu Val Glu
100 105 110
Arg Pro Phe Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Lys Arg Val
115 120 125
Glu Gln Met Glu Asp Arg Leu Lys Glu Asp Val Leu Leu Glu Ala Ala
130 135 140
Arg Tyr Gly Asn Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln
145 150 155 160
Met Val Asp Gln Trp Glu Pro Val Thr Ser Asp Ser Val Lys Thr Pro
165 170 175
Leu Gln Val Tyr Glu Glu Leu Gln Ala Gln Glu Tyr Leu Val Asp Tyr
180 185 190
Glu Arg Val Pro Ile Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe
195 200 205
Asp Ile Leu Val Ser Ile Cys
210 215
<210> 66
<211> 412
<212> PRT
<213> 葡萄(Vitis vinifera)
<400> 66
Met Ser Ile Pro Arg Glu Pro Glu Gln Val Met Lys Leu Arg Gly Gly
1 5 10 15
Ser Val Leu Gly Arg Lys Thr Ile Leu Lys Ser Asp His Phe Pro Gly
20 25 30
Cys Gln Asn Lys Arg Leu Ser Pro Gln Ile Asp Gly Ala Pro Asn Tyr
35 40 45
Arg Gln Ala Asp Ser Val His Val His Gly Val Ala Ile Pro Thr Ile
50 55 60
Asp Gly Ile Arg Asn Val Leu Glu His Ile Gly Ala Gln Val Asp Arg
65 70 75 80
Lys Gln Thr Gln Val Leu Trp Ile Asn Leu Arg Glu Glu Pro Val Val
85 90 95
Tyr Ile Asn Gly Arg Pro Phe Val Leu Arg Asp Val Glu Arg Pro Phe
100 105 110
Ser Asn Leu Glu Tyr Thr Gly Ile Asn Arg Ala Arg Val Glu Gln Met
115 120 125
Glu Ala Arg Leu Lys Glu Asp Ile Leu Met Glu Ala Ala Arg Tyr Gly
130 135 140
Tyr Lys Ile Leu Val Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp
145 150 155 160
Gln Trp Glu Pro Val Ser Arg Asp Ser Val Lys Thr Pro Leu Glu Val
165 170 175
Tyr Glu Glu Leu Gln Val Glu Gly Tyr Leu Val Asp Tyr Glu Arg Val
180 185 190
Pro Val Thr Asp Glu Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu
195 200 205
Val His Lys Ile Ser Gln Ala Asn Ile Asn Thr Glu Ile Ile Phe Asn
210 215 220
Cys Gln Met Gly Arg Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr
225 230 235 240
Leu Val Tyr Leu Asn Arg Ile Gly Ala Ser Gly Met Pro Arg Ser Asp
245 250 255
Ser Ile Gly Lys Val Phe Asp Ser Gly Thr Asn Val Ser Asp His Leu
260 265 270
Pro Asn Ser Glu Glu Ala Ile Arg Arg Gly Glu Tyr Ala Ala Ile Arg
275 280 285
Ser Leu Ile Arg Val Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val
290 295 300
Asp Lys Val Ile Asp Lys Cys Ala Ser Met Gln Asn Leu Arg Glu Ala
305 310 315 320
Ile Ala Thr Tyr Arg Asn Ser Ile Leu Arg Gln Arg Asp Glu Met Lys
325 330 335
Arg Glu Ala Leu Leu Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr
340 345 350
Phe Leu Ile Cys Phe Ala Val Tyr Ile His Thr Asp Arg Ala Ala Leu
355 360 365
His Pro Asp Ser Phe Gly His Ser Ser Phe Ala Asp Trp Met Arg Ala
370 375 380
Arg Pro Glu Leu Tyr Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro
385 390 395 400
Met Gly Ala Leu Gly Tyr Ala Asn Leu Glu Pro Ser
405 410
<210> 67
<211> 391
<212> PRT
<213> 番茄(Solanum lycopersicum)
<400> 67
Met Arg Ser Met Ser Ile Pro Lys Glu Pro Glu Gln Val Met Lys Gln
1 5 10 15
Arg Asp Gly Ser Val Leu Gly Lys Lys Thr Ile Leu Lys Ser Asp His
20 25 30
Phe Pro Gly Cys Gln Asn Lys Arg Leu Ser Pro His Ile Asp Gly Ala
35 40 45
Pro Asn Tyr Arg Lys Ala Ala Ser Leu His Val His Gly Val Ala Ile
50 55 60
Pro Thr Val Glu Gly Ile Gln Asn Val Leu Asp His Ile Gly Thr Gln
65 70 75 80
Leu Ser Gly Lys Lys Thr His Ile Leu Trp Ile Asn Leu Arg Glu Glu
85 90 95
Pro Gly Ile Asn Arg Thr Arg Val Glu Glu Met Glu Asp Arg Leu Lys
100 105 110
Asp Asp Val Leu Gln Glu Ala Ala Arg Tyr Gly Asn Lys Ile Leu Val
115 120 125
Thr Asp Glu Leu Pro Asp Gly Gln Met Val Asp Gln Trp Glu Pro Val
130 135 140
Thr Tyr Asp Ser Val Lys Thr Pro Leu Gln Val Tyr Glu Glu Leu Gln
145 150 155 160
Thr Lys Glu Tyr Leu Val Glu Tyr Glu Arg Val Pro Ile Thr Asp Glu
165 170 175
Lys Ser Pro Lys Glu Leu Asp Phe Asp Ile Leu Val His Arg Val Ser
180 185 190
Gln Ala Asp Val Asn Thr Gln Ile Ile Phe Asn Cys Gln Met Gly Arg
195 200 205
Gly Arg Thr Thr Thr Gly Met Val Ile Ala Thr Leu Val Tyr Leu Asn
210 215 220
Arg Ile Gly Ala Ser Gly Ile Pro Arg Ser Asn Ser Ile Gly Arg Val
225 230 235 240
Ser Asp Cys Ile Ser Asn Leu Asn Asp Thr Leu Ala Asn Ser Glu Glu
245 250 255
Ala Ile Arg Arg Gly Glu Tyr Thr Val Ile Arg Ser Leu Ile Arg Val
260 265 270
Leu Glu Gly Gly Val Glu Gly Lys Arg Gln Val Asp Lys Val Ile Asp
275 280 285
Lys Cys Ser Ser Met Gln Asn Leu Arg Glu Ala Ile Ala Ile Tyr Arg
290 295 300
Asn Ser Ile Leu Arg Gln Pro Asp Glu Met Lys Arg Glu Ala Ala Leu
305 310 315 320
Ser Phe Phe Val Glu Tyr Leu Glu Arg Tyr Tyr Phe Leu Ile Cys Phe
325 330 335
Ala Val Tyr Leu His Thr Gln Arg Asp Ala Leu Phe Ala Gly Ser Ser
340 345 350
Ala His Cys Ser Phe Ser Asp Trp Met Lys Ala Arg Pro Glu Leu Tyr
355 360 365
Ser Ile Ile Arg Arg Leu Leu Arg Arg Asp Pro Met Gly Ala Leu Gly
370 375 380
Tyr Val Ser Leu Glu Pro Ser
385 390

Claims (8)

1.一种蛋白，其特征在于，所述蛋白的氨基酸序列如SEQ ID NO.1-SEQ ID NO.3所示；或者，所述蛋白的氨基酸序列具有与SEQ ID NO.1所示序列至少70％序列相似度的蛋白；任选地，所述蛋白的氨基酸序列如SEQ ID NO.6至SEQ ID NO.67中的任意一个所示。
2.核酸，其特征在于，所述的核酸编码权利要求1所述的蛋白；任选地，所述的核酸序列如SEQ ID NO.4和SEQ ID NO.5所示。
3.基因表达盒，其特征在于，所述表达盒含有权利要求2所述的核酸序列；任选地，所述核酸与ubiquitin启动子和nos终止子可操作地连接。
4.表达载体，其特征在于，含有权利要求3所述的表达盒；任选地，所述载体还包含ubiquitin:bar:CaMV35S polyA表达盒。
5.宿主细胞，其特征在于，包含权利要求4所述的表达载体；任选地，所述宿主细胞为植物细胞或原核生物细胞；任选地，所述宿主细胞为大肠杆菌或农杆菌细胞。
6.产生转基因植物的方法，其特征在于，用权利要求4所述的表达载体或权利要求5所述的宿主细胞转化植物，得到转基因植物。
7.产生转基因种子的方法，其特征在于，从权利要求6所述方法产生的转基因植物产生转基因种子。
8.权利要求1至权利要求7所述的蛋白、核酸、基因表达盒、表达载体、宿主细胞、方法在提高植物抗旱性上的应用；任选地，所述植物为双子叶植物和单子叶植物；任选地，所述植物为拟南芥和玉米。
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CN113481316A (zh) * 2021-07-15 2021-10-08 华中农业大学 玉米抗旱标记dresh8及其应用
CN114231535A (zh) * 2021-11-26 2022-03-25 广东省科学院南繁种业研究所 木薯MeRSZ21b基因在提高植物抗干旱胁迫中的应用
CN114231535B (zh) * 2021-11-26 2023-08-25 广东省科学院南繁种业研究所 木薯MeRSZ21b基因在提高植物抗干旱胁迫中的应用

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