CN110608934A - Independent dehydration embedding assembly and use method thereof - Google Patents
Independent dehydration embedding assembly and use method thereof Download PDFInfo
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- CN110608934A CN110608934A CN201910996844.9A CN201910996844A CN110608934A CN 110608934 A CN110608934 A CN 110608934A CN 201910996844 A CN201910996844 A CN 201910996844A CN 110608934 A CN110608934 A CN 110608934A
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- 230000018044 dehydration Effects 0.000 title claims abstract description 60
- 238000006297 dehydration reaction Methods 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 99
- 238000000605 extraction Methods 0.000 claims abstract description 33
- 238000001914 filtration Methods 0.000 claims abstract description 31
- 238000007598 dipping method Methods 0.000 claims abstract description 11
- 208000005156 Dehydration Diseases 0.000 claims description 58
- 229940057995 liquid paraffin Drugs 0.000 claims description 24
- 239000012188 paraffin wax Substances 0.000 claims description 17
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 15
- 239000012024 dehydrating agents Substances 0.000 claims description 12
- 239000001993 wax Substances 0.000 claims description 11
- 230000003014 reinforcing effect Effects 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000000429 assembly Methods 0.000 claims description 3
- 230000000712 assembly Effects 0.000 claims description 3
- 238000007711 solidification Methods 0.000 claims description 3
- 230000008023 solidification Effects 0.000 claims description 3
- 238000012864 cross contamination Methods 0.000 abstract description 6
- 238000003745 diagnosis Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 119
- 230000002349 favourable effect Effects 0.000 description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 238000004018 waxing Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses an independent dehydration embedding assembly and a using method thereof, wherein the assembly comprises a reagent accommodating box, a sample placing box and an embedding bracket; the embedding bracket is stacked on the sample placing box, and a gap is formed between the embedding bracket and the bottom of the sample placing box; the sample placing box is stacked on the reagent accommodating box, and a clamping component for adjusting a gap between the bottom of the sample placing box and the bottom of the reagent accommodating box is arranged between the sample placing box and the reagent accommodating box; the bottom of the sample placing box is provided with a first filtering through hole; the embedding support is provided with a second filtering through hole, and the reagent accommodating box is communicated with an extraction part for extracting the dehydrated embedding reagent in the reagent accommodating box. The assembly can independently carry out fixing, dehydrating, transparent and wax-dipping embedding treatment on each biological tissue sample, and the phenomenon of cross contamination is not easy to occur in the fixing, dehydrating, transparent and wax-dipping embedding treatment process, so that the assembly has the advantage of avoiding false positive in diagnosis.
Description
Technical Field
The invention relates to the technical field of biological sample tissue treatment, in particular to an independent dehydration embedding component and a using method thereof.
Background
Before the biological tissue sample is diagnosed, the biological tissue sample needs to be processed by steps of fixing, dehydrating, transparentizing, waxing, embedding, slicing, dyeing and the like, so that a better cell slice image can be finally obtained, and the diagnosis is convenient. In the above process, the biological tissue sample is usually dehydrated by a dehydrator, and then embedded by an embedding machine, and finally sliced and stained.
That is, the specific operation is usually to place the biological tissue sample in an embedding box, stack the multiple embedding boxes in order in a dehydrating machine, and then sequentially add in the embedding box and sequentially fix the tissue cells with a fixing agent (such as formalin); then, the dehydrating agent is adopted to replace the water in the cells of the biological tissue sample; then, the alcohol in the cells is replaced by transparent liquid (such as dimethylbenzene), and then the transparent liquid in the cells of the biological tissue sample is replaced by liquid paraffin, so that the operations of fixing, dehydrating, transparentizing and wax-dipping of the biological tissue sample are realized; then, the processed biological tissue sample and the embedding box are taken out together and placed in an embedding machine to carry out embedding treatment on the biological tissue sample to form a tissue wax block, and then the tissue wax block is sliced and stained.
Wherein, the embedding box includes the box body and connects the lid on the box body, and has all seted up the through-hole on lid and box body.
However, when the above prior art is used to perform fixing, dehydrating, transparentizing, and waxing operations, since it is necessary to stack a plurality of dehydration boxes containing different biological tissue samples in a dehydrating machine, and then sequentially inject the same batch of processing reagents such as fixing agent, dehydrating agent, transparent liquid, liquid paraffin, etc. for processing, in the process of the batch operation, although the sample processing effect can be achieved, debris in different biological tissue samples is easily brought into other embedding boxes for placing the biological tissue samples along with the flow of the processing reagents, and finally cross contamination between the biological tissue samples is caused, so that a false positive result appears in the diagnosis result after the final tissue wax block section is stained.
Disclosure of Invention
Aiming at the defects in the prior art, the first purpose of the invention is to provide an independent dehydration embedding assembly, which can independently perform the fixation, dehydration, transparence and wax-dipping embedding treatment on each biological tissue sample, is not easy to cause the phenomenon of cross contamination in the fixation, dehydration, transparence and wax-dipping embedding treatment processes, and has the advantage of avoiding the occurrence of false positive in diagnosis.
The second purpose of the invention is to provide a using method of the independent dehydration embedding assembly, which can be completed in the same assembly when the operations such as fixing, dehydrating, transparentizing, wax-dipping embedding and the like are carried out, and the biological tissue sample does not need to be transferred in the operation process, so that the operation is simpler and more convenient.
In order to achieve the first object, the invention provides the following technical scheme:
an independent dehydration embedding assembly comprises a reagent accommodating box, a sample placing box and an embedding bracket; the reagent accommodating box is provided with a first cavity, the sample placing box is provided with a second cavity, and the embedding bracket is provided with a third cavity;
the embedding bracket is stacked on the sample placing box, and a gap is formed between the embedding bracket and the bottom of the sample placing box; the sample placing box is stacked on the reagent accommodating box, and a clamping component for adjusting a gap between the bottom of the sample placing box and the bottom of the reagent accommodating box is arranged between the sample placing box and the reagent accommodating box;
the bottom of the sample placing box is provided with a first filtering through hole; the embedding support is provided with a second filtering through hole, and the reagent accommodating box is communicated with an extraction part for extracting the dehydrated embedding reagent in the reagent accommodating box.
By adopting the technical scheme, the reagent accommodating box can be stacked in the first cavity of the reagent accommodating box, and reagents (such as a fixing agent, a dehydrating agent, a transparent liquid and the like) for dehydrating and embedding are added. In the dehydration treatment process, the area formed by the first filtering through hole on the sample placing box is used for placing the biological tissue sample, and the first cavity in the reagent containing box is not only used for stacking the sample placing box, but also used for injecting a reagent for dehydration embedding to dehydrate the biological tissue sample. The embedded bracket can be used for limiting the biological tissue sample placed in the sample placing box in a proper space range, so that the biological tissue sample is relatively flatly contacted with the bottom of the sample placing box.
The reagent for dehydration embedding that adds enters into the second cavity of sample box through second filter through-hole to in entering into reagent holding box through first filter through-hole, when the reagent for dehydration embedding that adds surpasses biological tissue sample, can carry out abundant dehydration to biological tissue sample. And the extraction part communicated with the reagent accommodating box can be used for extracting and removing the reagent for dehydration and embedding, thereby completing the dehydration treatment. In the process of wax immersion and embedding, the liquid paraffin is immersed and embedded on the second filtering through hole of the embedding bracket, so that the contact area between the liquid paraffin and the embedding bracket is increased, the paraffin can be kept fully connected with the embedding bracket after being cooled and solidified, and subsequent slicing is facilitated.
In this application, need not to use hydroextractor and embedding machine respectively, can accomplish the operation of dehydration and embedding in the independent dehydration embedding subassembly that holds box, sample placement box and embedding support to constitute by reagent to help making the operation more convenient and realize automated operation more easily. In the process of dehydration treatment, each biological tissue sample can be independently dehydrated, so that mutual cross contamination caused by the use of the same batch of reagents (such as a fixing agent, a dehydrating agent, a transparent liquid and the like) for dehydration and embedding among the biological tissue samples can be avoided, and the accuracy of subsequent diagnosis can be improved.
More preferably: the clamping component comprises a first limiting block and a clamping block, the first limiting block is arranged in a first cavity of the reagent containing box, and the clamping block is arranged outside the sample containing box.
By adopting the technical scheme, when the clamping block is positioned above the first limiting block and is abutted against the first limiting block, a gap is formed between the bottom of the sample placing box and the bottom of the reagent containing box, and when the reagent for dehydration embedding is added, the reagent for dehydration embedding firstly passes through the second filtering through hole, then enters the sample placing box and passes through the first filtering through hole, and finally enters the reagent containing box, so that the biological tissue sample can be fully dehydrated; when the card piece was located first stopper below and first stopper butt, the bottom that the box was placed to the sample and the bottom butt that the box was held to reagent, at this moment, was convenient for make the liquid paraffin of pouring into form smooth terminal surface after the cooling solidification.
More preferably: the extraction portion includes the extraction casing and sets up the extraction chamber on the extraction casing, extraction chamber and first cavity intercommunication.
Through adopting above-mentioned technical scheme, because the extraction portion holds the box for protruding setting on the reagent, and the dehydration embedding reagent is located first cavity, after certain step (if fixed, dehydration, transparent) in the dehydration operation ended, can directly extract out through the dehydration embedding reagent that the extraction chamber corresponds in with first cavity to other kinds of fixed, dehydration, transparent the dehydration operation that corresponds once more is put into again in follow-up step, or the end dehydration is and is carried out the wax immersion embedding processing.
More preferably: the bottom of the reagent accommodating box is inclined downwards from one end far away from the extraction part to one end of the extraction part and forms a drainage surface.
Through adopting above-mentioned technical scheme, hold in the reagent holds the box and dewater embedding with reagent (like fixative, dehydrating agent, transparent liquid etc.), the drainage face helps to collect the embedding with reagent of dewatering in lower one end, collects the one end of extraction portion promptly to be convenient for extract totally with the embedding with reagent of dewatering in the third cavity, make each step of dehydration processing step can both reach better effect.
More preferably: the bottom of the reagent accommodating box is provided with a protruding structure, and when the bottom of the sample accommodating box is abutted against the bottom of the reagent accommodating box, the protruding structure is embedded in the first filtering through hole.
Through adopting above-mentioned technical scheme, protruding structure inlays locates in the first filtration through-hole to the up end of protruding structure flushes with the bottom surface that the box was placed to the sample, at the in-process of embedding, is favorable to making the terminal surface of the paraffin after the cooling shaping keep level and smooth, is convenient for subsequent section operation. The protruding structures may be the structures of bars, dots, bumps, etc.
More preferably: the sample placing box is provided with a supporting table, the embedding support is provided with a placing surface, and the placing surface is abutted to the supporting table.
Through adopting above-mentioned technical scheme, when placing face and a supporting bench butt, the embedding support is fixed on the box is placed to the sample steadily, when the embedding of waxing is handled, is favorable to reducing the infiltration of paraffin in the clearance part of the side that the box was placed to embedding support and sample to reduce paraffin cooling and solidify back embedding support and sample and place the adhesion between the box, be convenient for place the box separation and take out with the embedding support together smoothly with the paraffin piece with the sample.
More preferably: the sample placing box is made of a material with the shrinkage rate smaller than that of paraffin.
By adopting the technical scheme, the shrinkage rate of the material is smaller than that of paraffin, such as metal, ceramic, glass and the like, and the stainless steel in the metal is more suitable for being used as a sample placing box due to stable performance. After the paraffin wax is used for manufacturing the sample placing box, after the liquid paraffin wax is cooled and solidified, the paraffin wax is easy to separate from the metal material, thereby being convenient for taking out the embedding bracket after the wax immersion embedding treatment and the biological tissue sample embedded and fixed on the embedding bracket through the paraffin wax.
More preferably: the embedding bracket is provided with a placing opening.
Through adopting above-mentioned technical scheme, when not placing the mouth, the embedding support can place the biological tissue sample restriction in a suitable space in the box is placed to the sample to make the sample keep comparatively level and smooth state, be favorable to going on smoothly of follow-up section operation. When the embedding bracket is provided with the placing port, the process of placing the biological tissue sample is more convenient. However, when the biological tissue sample is elongated and is located opposite to the placement opening, the biological tissue sample is prone to be erected, and an effective biological tissue slice cannot be obtained during the slicing operation.
More preferably: and a reinforcing rib is arranged in the third cavity, and a handheld block is arranged on the embedding support in a protruding mode.
By adopting the technical scheme, when the embedding bracket is taken out, the protruding handheld block is grasped, so that the embedding bracket and the biological tissue sample fixed on the embedding bracket can be taken out smoothly together. Meanwhile, the handheld block can also play a role in printing or pasting the mark.
And the setting of strengthening rib is favorable to improving the bulk strength of embedding support, and still can contact with liquid paraffin simultaneously to filter the through-hole with the second and mutually support, be favorable to further increasing the joint strength between liquid paraffin and the embedding support.
In order to achieve the second object, the invention provides the following technical scheme:
a method of using a self-contained dehydration encapsulation assembly comprising the steps of:
step one, stacking the independent dehydration embedding assemblies and placing the biological tissue sample: stacking the reagent accommodating box and the sample placing box, stacking the sample placing box in the reagent accommodating box, placing the biological tissue sample on the area formed by the first filtering through hole, and stacking the embedding bracket in the sample placing box;
step two, dehydration treatment: adding the fixing agent into the reagent containing box and submerging the biological tissue sample, and removing the fixing agent through the extraction cavity, wherein the operation can be repeatedly carried out; respectively treating the biological tissue sample by adopting a dehydrating agent and a transparent liquid in the same way in sequence;
step three, wax dipping and embedding treatment: under the constant temperature condition will independently dewater embedding subassembly and biological tissue sample and place in, add the second filter through-hole on the embedding support in the reagent holds the box with liquid paraffin, after biological tissue sample is fully soaked by liquid paraffin, press down the embedding support and place the bottom of box and the bottom butt that the box was held to the reagent to the sample, the cooling solidifies, the separation takes out the embedding support that has biological tissue sample and paraffin.
Through adopting above-mentioned technical scheme, in step one, place the box with the sample earlier, reagent holds the box and stacks, places biological tissue sample on the sample places the area that first filtration through-hole formed in the box, stacks the embedding support in the sample places the box again to make biological tissue sample can comparatively flatly place the sample in and place the box.
The dehydration treatment in the second step respectively comprises the following operation steps of fixing, dehydrating and transparentizing in sequence, namely: fixing the tissue cells with a fixative (e.g., formalin); the dehydrating agent is adopted to replace the water in the cells; using transparent liquid (such as xylene) to displace alcohol from cells; the clear liquid in the cells was displaced with liquid paraffin. During operation, the fixing agent is added into the reagent containing box and is submerged in the biological tissue sample, so that the fixing agent is fully contacted with the biological tissue sample, tissue cells are fully fixed, and the fixing agent is extracted through the extraction cavity after standing or oscillation operation. And then the dehydrating agent and the transparent liquid are added and removed by the same operation mode. After the fixing agent, the dehydrating agent and the transparent liquid are completely removed, the same reagent for dehydrating and embedding can be added again for corresponding operation, and finally, a good dehydrating effect on the biological tissue sample is achieved.
In the third step, the added liquid paraffin enters the sample placing box through the second filtering through hole and then enters the reagent containing box through the first filtering through hole, and when the liquid paraffin does not pass through the second filtering through hole, the constant temperature condition is adopted, so that the liquid paraffin is kept in a liquid state, the biological tissue sample can be fully coated by the liquid paraffin, and xylene in cells is replaced. After liquid paraffin and biological tissue sample fully contacted, press down the embedding support to the bottom that the box was placed to the sample and hold the bottom butt of box with the reagent, make the protruding structure inlay locate in first filtering through-hole to the up end of protruding structure and the bottom surface that the box was placed to the sample flush. And then the liquid paraffin is cooled and solidified, the biological tissue sample is firmly fixed at the bottom of the embedding bracket, and a flat surface is formed, so that subsequent processing such as slicing, dyeing and the like can be more conveniently carried out.
In conclusion, the invention has the following beneficial effects:
in the invention, the operation of dehydration and embedding can be completed in the independent dehydration and embedding component consisting of the reagent containing box, the sample placing box and the embedding bracket without respectively using a dehydrator and an embedding machine, thereby being beneficial to facilitating the operation and easily realizing the automatic operation. In the dehydration treatment process, each biological tissue sample can be independently dehydrated, so that the mutual cross contamination caused by the use of the same batch of reagents for dehydration embedding among the biological tissue samples can be avoided, and the improvement of the accuracy of subsequent observation and detection is facilitated.
According to the paraffin wax embedding device, when the bottom of the sample placing box is abutted to the bottom of the reagent containing box, the protruding structure at the bottom of the reagent containing box is embedded in the first filtering through hole, the upper end face of the protruding structure is flush with the bottom face of the sample placing box, and in the embedding process, the end face of the cooled and formed paraffin wax is kept flat, so that subsequent slicing operation is facilitated.
According to the invention, the drainage surface in the reagent accommodating box is helpful for collecting the reagent for dehydration and embedding to the lower end, namely the end of the extraction part, so that the reagent for dehydration and embedding in the third cavity can be conveniently and cleanly extracted, and a good effect can be achieved in each dehydration treatment.
According to the invention, the bottom of the embedding bracket is provided with the second filtering through hole, and the third cavity is internally provided with the reinforcing rib, so that the contact area between the added sealing agent and the embedding bracket in the embedding process is further increased, and the bonding firmness between the wax block and the embedding bracket is further improved.
According to the invention, the operation methods of fixing, dehydrating, transparentizing, wax-dipping embedding and the like are carried out on the biological tissue sample by adopting the operation method of matching the embedding bracket, the sample placing box and the reagent containing box together, the biological tissue sample does not need to be transferred to an embedding machine from a dehydrator, so that the operation is simpler and more convenient, and the automatic operation is easier to realize.
Drawings
FIG. 1 is a schematic view showing the structure of a reagent storage case, a sample storage case, and an embedding holder stacked in example 1 of the present invention;
FIG. 2 is an exploded view of example 1 of the present invention;
FIG. 3 is a schematic structural view of an embedding bracket in embodiment 1 of the present invention;
FIG. 4 is a schematic view of the construction of a reagent-containing cartridge in example 1 of the present invention;
FIG. 5 is a sectional view of a reagent accommodating case in example 1 of the present invention;
FIG. 6 is a schematic view showing the constitution in which the reagent accommodating case and the sample containing case are stacked in example 1 of the present invention;
FIG. 7 is a schematic view showing the constitution of a reagent accommodating case, a sample-placing case and an embedding holder when they are stacked in example 2 of the present invention.
In the figure, 1, a reagent accommodating box; 2. a sample placement box; 3. embedding the stent; 4. a first cavity; 5. a second cavity; 6. a third cavity; 7. a clamping component; 71. a first stopper; 72. a clamping block; 8. a placement port; 9. an extraction section; 91. extracting the shell; 92. an extraction lumen; 10. a drainage surface; 11. a protruding structure; 12. a first filter through hole; 13. a second filter through hole; 14. a support table; 15. placing the noodles; 16. reinforcing ribs; 17. a hand-held block; 18. and (4) a notch.
Detailed Description
The present invention will be described in further detail with reference to the following drawings and examples.
Example 1: an independent dehydration embedding assembly, as shown in figures 1 and 2, comprises a reagent containing box 1, a sample placing box 2 and an embedding bracket 3 from bottom to top in sequence, wherein the reagent containing box 1 and the embedding bracket 3 are both made of plastic materials, and the sample placing box 2 is made of stainless steel materials. Wherein, first cavity 4 has been seted up to reagent holding box 1, and second cavity 5 has been seted up to sample placing box 2, and embedding support 3 has seted up third cavity 6. And the embedding holder 3 is stacked on the sample placing cassette 2 with a gap (not shown) between the embedding holder 3 and the bottom of the sample placing cassette 2. And because the outer wall of the sample placing box 2 is integrally connected with the clamping block 72 along the horizontal direction, and the first cavity 4 of the reagent containing box 1 is internally and integrally connected with the first limiting block 71 along the horizontal direction, when the sample placing box 2 is inserted into the first cavity 4, the clamping block 72 is positioned above the first limiting block 71 and forms butt joint with the first limiting block 71, so that the sample placing box 2 is stacked on the reagent containing box 1.
Referring to fig. 3, the embedding holder 3 is integrally connected to the holding blocks 17 along the horizontal direction at two side edges of the top along the length direction, and one of the holding blocks 17 is provided with a notch 18, and the notch 18 is located right above the extraction cavity 92 (not shown in the figure).
Referring to fig. 3, a square placing opening 8 is formed in the bottom of the embedding holder 3, and a second filter through hole 13 having a long strip shape is formed around the placing opening 8. At the bottom of the sample placing box 2, a strip-shaped first filtering through hole 12 is opened, and the shape of the area formed by the first filtering through hole 12 is square, and the size of the area is the same as that of the placing opening 8. A reinforcing rib 16 is integrally connected to each of the two sides of the placement opening 8 in the third cavity 6, and the reinforcing ribs 16 are arranged along the length direction of the embedding bracket 3 and have the same height as the third cavity 6.
Referring to fig. 2, a horizontal support table 14 is provided on the sample storage case 2, a horizontal placing surface 15 is provided on the embedding holder 3, and when the embedding holder 3 is placed in the second cavity 5, the placing surface 15 abuts against the support table 14, thereby realizing the stacking of the embedding holder 3 in the sample storage case 2. And at this time, the placing port 8 is located right above the region where the first filtering through-hole 12 is formed (not shown in the figure).
Referring to FIGS. 4 and 5, a flow guide surface 10 is formed at the bottom of the reagent holding cassette 1, sloping downward from the end away from the extraction portion 9 to the end of the extraction portion 9. A plurality of protruding structures 11 are integrally connected to the drainage surface 10 along the longitudinal direction thereof, and the shape and size of the region formed by the protruding structures 11 are the same as those of the region formed by the first dewatering channel. And an extraction housing 91 is integrally connected to one side of the reagent accommodating box 1, and an extraction chamber 92 communicating with the first cavity 4 is opened on the dehydration housing.
Referring to fig. 6, when the bottom of the sample-placing cassette 2 abuts against the bottom of the reagent-containing cassette 1, the projection structure 11 is a projection and is embedded in the first through-filtration hole 12.
The specific application method is as follows:
step one, stacking the independent dehydration embedding assemblies and placing the biological tissue sample: stacking the reagent accommodating box 1 and the sample placing box 2, stacking the sample placing box 2 in the reagent accommodating box 1, placing the biological tissue sample on the area formed by the first filtering through hole 12, and stacking the embedding bracket 3 in the sample placing box 2;
step two, dehydration treatment: the fixing agent is added into the reagent accommodating box 1 and is immersed in the biological tissue sample, and the fixing agent is removed through the extraction cavity 92, and the operation can be repeatedly carried out; respectively treating the biological tissue sample by adopting a dehydrating agent and a transparent liquid in the same way in sequence;
step three, wax dipping and embedding treatment: the independent dehydration embedding assembly and the biological tissue sample are placed under the constant temperature condition, liquid paraffin is added into the reagent containing box 1 and does not pass through the second filtering through hole 13 on the embedding support 3, after the biological tissue sample is fully soaked by the liquid paraffin, the embedding support 3 is pressed downwards until the bottom of the sample containing box 2 is abutted against the bottom of the reagent containing box 1, the cooling solidification is carried out, and the embedding support 3 with the biological tissue sample and the paraffin is separated and taken out.
In the process of dehydration embedding treatment, each biological tissue sample can be subjected to independent dehydration treatment, and cross contamination caused by the fact that the same batch of fixing agent, dehydrating agent and transparent liquid is adopted in the dehydration process of different biological tissue samples is not easy to cause, so that the biological tissue samples are favorably reduced in the subsequent detection process and false positive detection results are reduced.
Example 2: an independent dehydration embedding assembly is different from the embodiment 1 in that, referring to fig. 7, a placing opening 8 is not formed at the bottom of an embedding bracket 3, a reinforcing rib 16 is arranged along the width direction of the embedding bracket 3, and the reinforcing rib 16 is connected with the bottom of the embedding bracket 3 and two opposite side walls of the embedding bracket 3.
When the biological tissue sample is slender, the bottom of the embedding bracket 3 is not provided with the placing opening 8, the biological tissue sample can be flattened in the process of pressing the embedding bracket 3, the state of the biological tissue sample is kept flat in the subsequent wax dipping embedding treatment, and the subsequent operations of slicing, dyeing and the like are facilitated. And the reinforcing ribs 16 are arranged along the width direction of the embedding bracket 3, so that the whole embedding bracket 3 can obtain better strength.
Example 3: an independent dewatering embedding assembly is different from the embodiment 1 in that the bottom of the embedding bracket 3 is not provided with the second filtering through hole 13.
Although the second filtering through hole 13 is not formed, the liquid paraffin can enter the second cavity 5 of the embedding bracket 3 through the placing port 8, so that the contact area between the liquid paraffin and the embedding bracket 3 can be increased, and the liquid paraffin can stably fix the biological tissue sample on the embedding bracket 3.
Example 4: a separate dewatering and embedding assembly, which differs from embodiment 3 in that no reinforcing ribs 16 are provided in the third cavity 6.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (10)
1. An independent dehydration embedding assembly is characterized by comprising a reagent accommodating box (1), a sample placing box (2) and an embedding bracket (3); the reagent accommodating box (1) is provided with a first cavity (4), the sample placing box (2) is provided with a second cavity (5), and the embedding bracket (3) is provided with a third cavity (6);
the embedding bracket (3) is stacked on the sample placing box (2), and a gap is formed between the embedding bracket (3) and the bottom of the sample placing box (2); the sample placing box (2) is stacked on the reagent accommodating box (1), and a clamping component (7) for adjusting a gap between the bottom of the sample placing box (2) and the bottom of the reagent accommodating box (1) is arranged between the sample placing box (2) and the reagent accommodating box (1);
the bottom of the sample placing box (2) is provided with a first filtering through hole (12); the embedding support (3) is provided with a second filtering through hole (13), and the reagent accommodating box (1) is communicated with an extraction part (9) for extracting the dehydrated embedding reagent in the reagent accommodating box (1).
2. An independent dehydration embedding assembly according to claim 1, wherein the clamping assembly (7) comprises a first limit block (71), a clamping block (72), the first limit block (71) is disposed in the first cavity (4) of the reagent containing box (1), and the clamping block (72) is disposed outside the sample placing box (2).
3. A self-contained dewatering and embedding assembly according to claim 1, characterized in that the extraction section (9) comprises an extraction housing (91) and an extraction chamber (92) opening onto the extraction housing (91), the extraction chamber (92) communicating with the first cavity (4).
4. A self-contained dehydration embedding assembly according to claim 1, characterized in that the bottom of the reagent containing cassette (1) is inclined downward from the end far from the extraction portion (9) to the end of the extraction portion (9) and forms a drainage surface (10).
5. An independent dehydration embedding assembly according to claim 4, characterized in that the bottom of the reagent containing box (1) is provided with a protruding structure (11), and when the bottom of the sample placing box (2) is abutted with the bottom of the reagent containing box (1), the protruding structure (11) is embedded in the first filtering through hole (12).
6. An individual dewatering embedding assembly according to claim 1, characterized in that the sample placing box (2) is provided with a support table (14) and the embedding holder (3) is provided with a placing surface (15), the placing surface (15) abutting against the support table (14).
7. A self-contained dehydration embedding assembly according to claim 1 wherein said sample placement box (2) is made of a material with a shrinkage less than paraffin.
8. An independent dewatering embedding assembly according to claim 1, characterized in that the embedding support (3) is provided with a placing opening (8).
9. A self-contained dehydration embedding assembly according to claim 1, characterized in that a reinforcing rib (16) is arranged in the third cavity (6), and a hand-held block (17) is protrusively arranged on the embedding bracket (3).
10. A method of using a self-contained dewatering embedding assembly according to any one of claims 1-9, characterised by the steps of:
step one, stacking the independent dehydration embedding assemblies and placing the biological tissue sample: stacking the reagent accommodating box (1) and the sample placing box (2), stacking the sample placing box (2) in the reagent accommodating box (1), placing the biological tissue sample on an area formed by the first filtering through hole (12), and stacking the embedding bracket (3) in the sample placing box (2);
step two, dehydration treatment: adding the fixing agent into the reagent accommodating box (1) and submerging the biological tissue sample, and removing the fixing agent through the extraction cavity (92), wherein the operation can be repeatedly carried out; respectively treating the biological tissue sample by adopting a dehydrating agent and a transparent liquid in the same way in sequence;
step three, wax dipping and embedding treatment: the independent dehydration embedding assembly and the biological tissue sample are placed under a constant temperature condition, liquid paraffin is added into the reagent containing box (1) and does not pass through the second filtering through hole (13) on the embedding support (3), after the biological tissue sample is fully soaked by the liquid paraffin, the embedding support (3) is pressed downwards until the bottom of the sample containing box (2) is abutted against the bottom of the reagent containing box (1), and the embedding support (3) with the biological tissue sample and the paraffin is separated and taken out after cooling and solidification.
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