JP2000275152A - Frame cassette for inspecting pathological organization - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a frame cassette for inspecting a pathological organization that prevents a cassette block from being lifted from the mounted state when mounting the cassette block using a frame cassette for inspecting the pathological organization to a microtome, slicing it into thin pieces, and creating a microscope sample. SOLUTION: In a frame cassette 20 for inspecting a pathological organization, a peripheral wall part 21 and a projecting end part 22 made of a synthetic resin with chemical resistance and low-temperature resistance are provided, the peripheral wall part 21 is in a square shape where an upper surface is open, a number of pores 23 or a reinforcing rib are provided at a bottom part, and at the same time the projecting end part 22 is in a plate-shaped body and is extended from the opposite side of the bottom part of the peripheral wall part 21 toward the outside. In the frame cassette 20, a means for preventing the frame cassette 20 from being lifted from the mounted state to a microtome is formed at the outer periphery of the peripheral wall part 21.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a frame cassette for pathological tissue examination used for preparing a microscope specimen for pathological tissue examination and for cryopreservation of the specimen, and more particularly, to a microtome for preparing a microscope specimen. The present invention relates to a frame cassette for histopathological examination for producing a predetermined number of specimens having a uniform thickness, which is prevented from floating from an attached state.

[0002]

2. Description of the Related Art Conventionally, as shown in FIGS. 22 and 23, a frame cassette of this type has a peripheral wall portion 1 made of synthetic resin having chemical resistance and low temperature resistance and a protruding edge portion 2. It is. The peripheral wall portion 1 is a rectangular container having an open upper surface, and has a large number of pores 3 or reinforcing ribs 4 formed at a bottom portion. The protruding edge 2 is a plate-like body and extends outward from the opposite side at the bottom of the peripheral wall 1.

In order to prepare a microscope sample using the frame cassette, in the case of the frame cassette shown in FIG. 22, first, as shown in FIG. Is removed, and xylene is provided with an affinity for liquid paraffin 7 described later.

[0005] Next, as shown in FIG. 25, liquid paraffin 7 is placed in a stainless steel tray 6, the specimen 5 is taken out from the peripheral wall 1, and placed on the liquid paraffin 7. continue,
The frame cassette is placed on the step of the tray 6 and the liquid paraffin 7 is kept until the bottom of the peripheral wall 1 is immersed in the liquid paraffin 7.
Is added to the sample 5. The step portion of the tray 6 is formed in a rectangular shape so that the protruding edge portion 2 can be inserted. By removing the tray 6 after the liquid paraffin 7 has solidified, the specimen 5
A cassette block 8 (see FIG. 26) in which paraffin 7 having embedded therein is attached to the bottom of the peripheral wall 1 is obtained.

Next, as shown in FIGS. 26 and 27, the cassette block 8 is placed upside down on the base 11 of the adapter 10 mounted on the microtome 9, and an L-shaped cross-section is erected on the base 11. The peripheral wall 1 is fitted between the two projections 12 having a shape, and the peripheral wall 1 is held between the fixed arm 14 and the movable arm 15 provided on the base 11 by operating the lever 13. And fix it. Subsequently, the portion of the paraffin 7 in which the specimen 5 is embedded is sliced in the direction of arrow a or b in FIG. 27, and the sliced slice is subjected to staining and other predetermined processing to obtain a microscope specimen.

When the specimen 5 is stored frozen, as shown in FIG. 25, the tray 6 is immersed in liquid nitrogen, frozen before being removed, and stored at a predetermined storage temperature.

In the case of the frame cassette shown in FIG. 23, although not shown, a sample accommodating chamber having a pore at the bottom as described in, for example, Japanese Utility Model Publication No. 3-55090 and Japanese Utility Model Publication No. 5-40449. The test cassette provided with one or more test cassettes is attached to the open top of the frame cassette with the lid removed or opened, and the same washing, pouring of alcohol, xylene, and liquid paraffin as above is performed in the test chamber. A cassette block is obtained by performing an adding operation. Also in the case of the frame cassette shown in FIG. 22, the cassette for inspection can be attached to the opening on the upper surface, and operations such as washing with water can be performed to obtain a cassette block.

[0008]

However, when the microscope block is prepared by slicing the cassette block 8 using the above-mentioned conventional frame cassette into thin slices, the adapter 1 is used.
Since the frictional force between the fixed arm 14 and the movable arm 15 and the peripheral wall 1 of the frame cassette is not so large, the peripheral wall 1 slides upward during slicing and the cassette block 8
May rise from the base 11. If the cassette block 8 rises, there is a problem that the thickness of the microscope specimen becomes non-uniform, or as a result, a predetermined number of microscope specimens cannot be obtained. The present invention
It is an object of the present invention to improve the conventional frame cassette to eliminate such a problem.

[0009]

In order to achieve the above object, a frame cassette for pathological tissue examination according to the present invention comprises a peripheral wall portion made of a synthetic resin having chemical resistance and low temperature resistance and a protruding edge portion. Provided, the peripheral wall portion is a square with an open upper surface,
A path cassette for pathological tissue examination comprising a plurality of pores or reinforcing ribs at the bottom, and the protruding edge is a plate-like body and extends outward from an opposite side of the bottom of the peripheral wall portion. Means are formed on the outer periphery of the peripheral wall portion to prevent the surface from rising from the state of attachment to the microtome.

[0010] It is preferable that the lifting prevention means is composed of a plurality of lateral ridges or grooves, or is composed of an embossed surface such as satin finish. In addition, it is preferable that the peripheral wall is tapered in an inverted pyramid shape. Further, it is convenient to form a recording section for recording the name and the like of the subject at the protruding edge.

[0011]

When a cassette block is manufactured using the frame cassette of the present invention having the above structure, the cassette block is reliably prevented from being lifted when the microscope block is mounted on a microtome and sliced into thin slices to prepare a microscope specimen. can do.

As a preferred embodiment of the lifting prevention means, in the case where a plurality of lateral ridges or grooves are formed on the outer periphery of the peripheral wall of the frame cassette, the fixed arm and the movable arm of the adapter mounted on the microtome are provided. The leading end portion engages between the ridges or into the groove to prevent the cassette block from floating.

As another preferred embodiment, in the case where an embossed surface such as satin is formed on the outer periphery of a peripheral wall portion of a frame cassette, a locking claw at a tip of a fixed arm and a movable arm of an adapter and the embossed surface are formed. Since the frictional force between the cassette block and the surface is large, there is no possibility that the peripheral wall portion slides upward and the cassette block is lifted.

Further, in the case where the peripheral wall of the frame cassette has an inverted pyramid-shaped taper, the cassette block is mounted upside down on the base of the adapter at the time of slicing. In a state. Therefore, the locking claws of the fixed arm and the movable arm of the adapter press the peripheral wall portion downward, thereby preventing the cassette block from floating. Therefore, according to the present invention, the problem that the sample thickness becomes non-uniform due to the lifting of the cassette block during the preparation of the microscope sample, and a predetermined number of samples cannot be obtained due to the increase in thickness, is solved, and the efficiency is improved. A microscope sample can be prepared well.

[0015]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be described below with reference to the drawings, but it goes without saying that the present invention is not necessarily limited to these embodiments. Note that the same reference numerals are used for the same parts or the same elements in each drawing.

Embodiment 1 As shown in FIGS. 1 to 4, a frame cassette 20A of this embodiment includes a peripheral wall portion 21 and a protruding edge portion 22 made of a synthetic resin having chemical resistance and low temperature resistance. It is made. As the synthetic resin, polyacetal, polycarbonate and the like are preferable.

The peripheral wall portion 21 is a rectangular container having an open top surface, a large number of fine holes 23 at the bottom portion, an inverted pyramid-shaped taper 24 on the outer periphery, and a plurality of lateral directions on four surfaces. Are formed. Although not shown, a groove may be formed instead of the protrusion 25. The protruding ridge 22 is a plate-like body, and extends outward from the opposite side at the bottom of the peripheral wall portion 21, and has a matte recording portion 26 formed on both front and back surfaces at both ends. The recording unit 26 records the name of the subject and the like.

In order to prepare a microscope sample using the frame cassette 20A having the above structure, first, as shown in FIG. 5, a collected sample 27 is housed in the peripheral wall portion 21 and washed with water, and the sample 27 is washed with alcohol. Is removed, and xylene is provided with affinity for liquid paraffin 29 described later.

Next, as shown in FIG. 6, a liquid paraffin 29 is put in a tray 28 made of stainless steel, and the specimen 27 is taken out from the peripheral wall 21 and placed on the liquid paraffin 29.
Subsequently, the frame cassette 20A is placed on the step of the tray 28, and the liquid paraffin 29 is poured onto the sample 27 until the bottom of the peripheral wall 21 is immersed in the liquid paraffin 29. The step portion of the tray 28 is formed in a rectangular shape so that the protruding edge portion 22 is inserted.

After the liquid paraffin 29 has solidified, the tray 2
By removing the sample 8, a cassette block 30 (see FIG. 7) in which the paraffin 29 in which the sample 27 is embedded is attached to the bottom of the peripheral wall 21 is obtained.

Next, as shown in FIGS. 7 and 8, the cassette block 30 is placed upside down on the base 33 of the adapter 32 mounted on the microtome 31, and an L-shaped cross-section is erected on the base 33. The peripheral wall 21 is fitted between the two projections 34 having a shape, and the peripheral wall 21 is held between the fixed arm 36 and the movable arm 37 provided on the base 33 by operating the lever 35. And fix it. At that time, both arms 3
Engaging claws 38 formed at the tips of 6, 37 engage with the lateral ridges 25 formed on the peripheral wall 21 so as to bite. At the same time, since the cassette block 30 is mounted upside down, the taper 24 on the outer periphery of the peripheral wall portion 21 has a forward pyramid shape. Therefore, the locking claw 38 is attached to the peripheral wall 2.
1 is pressed downward. As a result, both arms 3
The locking claws 38 of the cassette blocks 6 and 37 are connected to the cassette block 3 described later.
At the time of slicing 0, the cassette block 30 is reliably prevented from rising from the base 33.

Subsequently, the portion of the paraffin 29 in which the specimen 27 is embedded is sliced in the direction of arrow A or B in FIG. 8, and the sliced slice is stained and subjected to other predetermined processing to obtain a microscope specimen.

When the specimen 27 is stored frozen,
As shown in FIG. 6, the tray 28 is immersed in liquid nitrogen, frozen before being removed, and stored at a predetermined storage temperature.

Embodiment 2 As shown in FIGS. 9 to 11, the construction of the frame cassette 20B of this embodiment is the same as that of Embodiment 1 (see FIG. 9) except that reinforcing ribs 39 are provided at the bottom of the peripheral wall 21 in a cross-girder shape. 1). Therefore, the ridge 25 and the taper 24 of the peripheral wall portion 21 operate in the same manner as in the first embodiment, and prevent the cassette block using the frame cassette 20B from floating.

Embodiment 3 As shown in FIG. 12, the frame cassette 20 of this embodiment
The configuration of Example C (see FIG. 9) is the same as that of Example 2 except that a plurality of continuous horizontal ridges 25 are formed on the entire outer periphery of the peripheral wall portion 21.
Reference). Therefore, the ridge 25 and the taper 24 of the peripheral wall portion 21 operate in the same manner as in the first embodiment, and prevent the cassette block using the frame cassette 20C from floating.

Embodiment 4 As shown in FIG. 13, the frame cassette 20 of this embodiment
The configuration of D is that a plurality of lateral ridges 25 are formed on two opposing surfaces of the peripheral wall portion 21 and a matte embossed surface 40 is formed on the remaining two opposing surfaces. This is the same as the first embodiment (see FIG. 1). Therefore,
The ridge 25 and the taper 24 of the peripheral wall 21 are the same as those in the first embodiment.
To prevent the cassette block using the frame cassette 20D from floating.

Although not shown, as another method of use, when the embossed surface 40 is sandwiched between the fixed arm 36 and the movable arm 37 of the adapter 32, both arms 3
A large frictional force acts between the locking pawls 38 and 6 and the embossed surface 40. Therefore, the frictional force and the taper 2
4 prevents the cassette block using the frame cassette 20D from floating.

Embodiment 5 As shown in FIG. 14, the frame cassette 20 of this embodiment
The configuration of E is the same as that of the fourth embodiment (see FIG. 13) except that the reinforcing ribs 39 are provided in the shape of a girder at the bottom of the peripheral wall portion 21. Therefore, the operation and effect of the ridge 25 and the embossed surface 40 of this embodiment are not different from those of the fourth embodiment.

Embodiment 6 As shown in FIG. 15, the frame cassette 20 of this embodiment
The configuration of F is different from that of the fifth embodiment (see FIG. 14) in that a plurality of laterally intermittent ridges 4 are used instead of the horizontal ridges 25.
This example is the same as Example 5 except that No. 1 was formed. The ridge 41 functions in a manner similar to the ridge 25 of the first embodiment (see FIG. 1). Therefore, the operation and effect of this embodiment are not different from those of the first embodiment.

Embodiment 7 As shown in FIG. 16, the frame cassette 20 of this embodiment
The configuration of G is the same as that of the fifth embodiment except that a plurality of rectangular ridges 42 are formed in a grid pattern instead of the horizontal ridges 25 in the fifth embodiment (see FIG. 14). . When the cassette block using the frame cassette 20G is sandwiched between the fixed arm 36 and the movable arm 37 of the adapter 32, the engaging claws 38 of the arms 36, 37 are engaged so as to bite between the rectangular projections 42. Combine. Therefore, the cassette block has a rectangular projection 42 as in the first embodiment.
And the action of the taper 24 prevents lifting. The operation and effect of the embossed surface 40 are described in the fourth embodiment.
Not different from

Embodiment 8 As shown in FIG. 17, the frame cassette 20 of this embodiment
The configuration of H is the same as that of the second embodiment (see FIG. 9) except that a matte embossed surface 40 is formed on the four outer peripheral surfaces of the peripheral wall portion 21. The embossed surface 40 acts in the same manner as the embossed surface 40 in the fourth embodiment (see FIG. 13), and together with the operation of the taper 24, prevents the cassette block using the frame cassette 20H from floating. .

Embodiment 9 As shown in FIGS. 18 and 19, the structure of the frame cassette 20I of this embodiment is the same as that of the first embodiment except that the peripheral wall portion 21 is erected perpendicularly to the protruding edge portion 22. This is the same as Example 2 (see FIG. 9). Therefore, the frame cassette 2
In the cassette block using 0I, the second embodiment
Does not have the effect of the taper 24 as in
The one ridge 25 acts in the same manner as in the second embodiment, and prevents the cassette block from floating.

Embodiment 10 As shown in FIGS. 20 and 21, the structure of the frame cassette 20J of this embodiment is the same as that of the embodiment except that a taper 43 of a regular pyramid shape is provided on the outer periphery of the peripheral wall portion 21. 2 (see FIG. 9). Therefore, when the cassette block using the frame cassette 20J is turned upside down and attached to the adapter 32, the taper 43 becomes an inverted pyramid shape, and the taper 24 does not have the effect of the second embodiment. The stop claw 38 is engaged so as to bite between the ridges 25 of the peripheral wall portion 21 to prevent the cassette block from floating.

[0034]

According to the present invention, as described above, the means for preventing the frame cassette cassette from rising is formed on the outer periphery of the peripheral wall portion by embossing surfaces such as lateral ridges or grooves or satin finishes. In addition, troubles such as a non-uniform thickness of the microscope specimen, a predetermined number of specimens being unable to be obtained due to an increase in the thickness, and the like can be prevented, and the microscope specimen can be efficiently manufactured. When the peripheral wall is tapered in the shape of an inverted pyramid, the locking claws of the fixed arm and the movable arm of the adapter press the peripheral wall of the cassette block downward, so that the ridges or grooves or embosses are formed. Together with the machined surface, there is an effect of increasing the lifting prevention effect.

[Brief description of the drawings]

FIG. 1 is a perspective view illustrating a frame cassette according to a first embodiment of the present invention.

FIG. 2 is a front view of the frame cassette according to the first embodiment.

FIG. 3 is a plan view of FIG. 2;

FIG. 4 is a sectional view taken along line IV-IV of FIG. 3;

FIG. 5 is a cross-sectional view illustrating a state where a sample is processed by the frame cassette according to the first embodiment.

FIG. 6 is an explanatory diagram showing one process of preparing a microscope specimen using the frame cassette of Example 1.

FIG. 7 is an explanatory diagram showing another process of preparing a microscope specimen using the frame cassette of Example 1.

FIG. 8 is a partially cutaway plan view of FIG. 7;

FIG. 9 is a perspective view illustrating a frame cassette according to a second embodiment of the present invention.

FIG. 10 is a plan view of a frame cassette according to a second embodiment.

11 is a sectional view taken along line XI-XI in FIG.

FIG. 12 is a perspective view illustrating a frame cassette according to a third embodiment of the present invention.

FIG. 13 is a perspective view illustrating a frame cassette according to a fourth embodiment of the present invention.

FIG. 14 is a perspective view showing a frame cassette according to a fifth embodiment of the present invention.

FIG. 15 is a perspective view illustrating a frame cassette according to a sixth embodiment of the present invention.

FIG. 16 is a perspective view showing a frame cassette according to a seventh embodiment of the present invention.

FIG. 17 is a perspective view illustrating a frame cassette according to an eighth embodiment of the present invention.

FIG. 18 is a perspective view illustrating a frame cassette according to a ninth embodiment of the present invention.

FIG. 19 is a front view of the frame cassette according to the ninth embodiment.

FIG. 20 is a perspective view illustrating a frame cassette according to a tenth embodiment of the present invention.

FIG. 21 is a front view of the frame cassette according to the tenth embodiment.

FIG. 22 is a perspective view showing an example of a conventional frame cassette.

FIG. 23 is a perspective view showing another example of the conventional frame cassette.

FIG. 24 is a cross-sectional view showing a state where a sample is processed by a conventional frame cassette.

FIG. 25 is an explanatory view showing one process of preparing a microscope sample using a conventional frame cassette.

FIG. 26 is an explanatory view showing another process of preparing a microscope specimen using a conventional frame cassette.

FIG. 27 is a partially cutaway plan view of FIG. 26;

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Peripheral wall part 2 Protrusion part 3 Micropore 4 Reinforcement rib 5 Sample 6 Tray 7 Paraffin 8 Cassette block 9 Microtome 10 Adapter 11 Base 12 Slope projection 13 Lever 14 Fixed arm 15 Movable arm 20A-J Frame cassette 21 Peripheral wall part 22 Protrusions 23 Pores 24 Tapers 25 Protrusions 26 Recording parts 27 Samples 28 Trays 29 Liquid paraffin 30 Cassette blocks 31 Microtomes 32 Adapters 33 Bases 34 Beveled protrusions 35 Lever 36 Fixed arm 37 Movable arm 38 Locking claw 39 Reinforcement rib 40 embossed surface 41 intermittent ridge 42 square protrusion 43 taper

Claims (5)

[Claims] 1. A peripheral wall portion made of a synthetic resin having chemical resistance and low temperature resistance and a protruding edge portion, wherein the peripheral wall portion has a rectangular shape with an open upper surface, and a plurality of pores or reinforcing ribs are provided at a bottom portion. And a protruding edge portion is a plate-like body, and in a pathological tissue inspection frame cassette extending outward from an opposite side of the bottom of the peripheral wall portion, the frame cassette rises from a state of being attached to a microtome. A frame cassette for pathological tissue examination, wherein a means for preventing the formation of the frame cassette is formed on the outer periphery of the peripheral wall portion. 2. The frame cassette for pathological tissue examination according to claim 1, wherein the lifting prevention means comprises a plurality of lateral ridges or grooves. 3. The frame cassette for pathological tissue examination according to claim 1, wherein the lifting preventing means is formed of an embossed surface such as a satin finish. 4. The frame cassette for pathological tissue examination according to claim 1, wherein the peripheral wall portion is tapered in an inverted pyramid shape. 5. The recording device according to claim 1, wherein a recording portion is formed at the protruding edge portion.
The frame cassette for pathological tissue inspection according to the above.