JP6473314B2 - Paraffin block making tool - Google Patents

Description

  The present invention relates to a paraffin block creating tool, and more particularly, to a paraffin block creating tool for providing a microscope specimen for preventing mistakes in a microscope specimen and increasing the reliability of inspection.

  A medical examination cassette (hereinafter sometimes simply referred to as a cassette) 11 used for producing this type of microscope specimen is a cassette body 12 made of a chemical-resistant synthetic resin, as shown in FIGS. And a lid 13. The cassette body 12 is a rectangular container having an open top surface, and has a plurality of through holes 14 on the bottom surface, and is a plate-shaped recording portion that is inclined outwardly toward the bottom surface on the outer side of one side wall on the short side. 12a is provided. The lid 13 is a detachable plate-like body and has a large number of through holes 14 on the plate surface. Reference numeral 15 denotes a locking portion between the lid 13 and the cassette body 12.

  In order to prepare a microscope specimen using the cassette 11, first, as shown in FIG. 14, the collected specimen S is accommodated in the cassette body 12, a lid 13 is attached, and the subject is attached to the recording unit 12a. Record names, symbols, etc. to identify

  Subsequently, the specimen S is washed with water through the through-hole 14 of the lid 13, the moisture in the specimen S is removed with alcohol, the fat content of the specimen S is removed with xylene, and affinity for liquid paraffin described later is imparted. .

  Next, liquid paraffin P is placed in a stainless steel tray T as shown in FIG. 15, the specimen S is taken out from the cassette body 12 and transferred to the tray T, and the liquid S is placed on the specimen S in the tray T. The specimen S is temporarily fixed. Subsequently, as shown in FIG. 16, the cassette body 12 is placed on the cassette mounting portion T <b> 2 of the tray T, and liquid paraffin P is further poured onto the specimen S until the cassette body 12 is immersed in the liquid paraffin P.

  Next, as shown in FIG. 17, after the liquid paraffin P is solidified, the tray T is removed to obtain a paraffin block B in which the paraffin P in which the specimen S is embedded adheres to the bottom surface of the cassette body 12.

  Next, although not shown, the paraffin block B is turned over and placed on the microtome, and the adapter installed on the microtome is engaged with the paraffin block B to fix the paraffin block B to the microtome. Subsequently, the portion in which the specimen S of paraffin P is embedded is sliced, and the obtained thin piece is stained and subjected to other predetermined processing, thereby obtaining a microscope specimen H as shown in FIG.

  By the way, as described above, in the recording unit 12a of the cassette body 12, the name and symbols of the subject are recorded, but the obtained specimen H is completely separated from the cassette body 12, Often, serious troubles such as mistaking the subject occur.

In order to eliminate such troubles, a cassette has been proposed in which identification indications such as numbers and alphabets are projected on the surface of the lid of the cassette (see, for example, Patent Document 1).
However, since this lid is also completely separated from the specimen, the trouble that the specimen is mistaken is not solved.

  In view of such circumstances, the present invention provides a paraffin block creating tool for solving the above-mentioned problems in conventional microscope specimen preparation.

Japanese Patent No. 4402265

  As a result of diligent investigations to solve the above-described problems of the prior art, the present inventors have found that if a specimen is provided with an identification display, the specimen (specimen) and the identification display are inseparably integrated, and confusing troubles are eradicated. It has been found and the present invention has been completed.

That is, the present invention is characterized in that a paraffin block in which a specimen is embedded in paraffin, comprising a medical examination cassette comprising a cassette body and a lid, a paraffin housing part and a cassette body mounting part provided at the periphery thereof. And a columnar body that does not melt at the liquefaction temperature of the paraffin and can be sliced, and means for standing the columnar body in the paraffin container of the tray, the columnar body being paraffin The paraffin block creating tool is characterized in that the means standing in the housing is a support provided at the bottom of the tray.

  Another feature of the present invention is a paraffin block making tool characterized in that the columnar body is selected from crayon, crepas, colored paraffin, rubber, elastomer, soft resin, and konjac.

  Another feature of the present invention is a paraffin block making tool characterized in that the columnar body is made of colored paraffin and has a melting point higher than that of the paraffin of the paraffin block body.

  Another feature of the present invention is a paraffin block making tool characterized in that the columnar body has a cross-sectional shape that can be identified.

  Another feature of the present invention is a paraffin block maker, wherein the identifiable cross-sectional shape is selected from letters, numbers, symbols, and patterns.

  Another feature of the present invention is a paraffin block making tool characterized in that the upper edge of the tray is erected obliquely or vertically.

  According to the paraffin block creating tool of the present invention, the columnar body having a color or an identifiable cross-sectional shape remains in the paraffin block embedding the specimen, and the columnar body has an identification display having a different color or cross-sectional shape. Is formed. Thus, when the paraffin block is sliced, identification indications having different colors and cross-sectional shapes of the sliced columnar bodies are formed in the paraffin slices including the sliced specimen. As a result, the specimen obtained by processing such as staining contains the identification display together with the specimen and is inseparably integrated. Therefore, the identification display is not separated, and the trouble of mistaking the specimen is solved.

  Moreover, as a means for standing the columnar body in the paraffin container, a support part provided in the paraffin container or a support part provided on the bottom rear surface of the cassette body is suitable.

  In addition, the columnar body is preferably crayon, crepas, colored paraffin, rubber, elastomer, soft resin, konjac, and crayon and crepas are particularly colored in 12 or 24 colors. It is preferable because it is possible. Among these, a columnar body made of colored paraffin and having a melting point higher than that of the paraffin of the paraffin block body is particularly preferable.

  Further, if necessary, identification display may be performed as an identifiable cross-sectional shape, and identification display may be performed by combining a color and a cross-sectional shape. Similarly, for rubber, elastomer, soft resin, and konjac, colors, cross-sectional shapes, and combinations thereof can be used as identification display.

  In addition, letters, numbers, symbols, and patterns are suitable as the identifiable cross-sectional shape.

  Further, by making the standing columnar body tapered so as to easily remain in the paraffin block, it is easy to remove the tray after the paraffin has solidified.

  In addition, by placing the upper edge of the tray diagonally or vertically, there is virtually no horizontal part where paraffin stays, and so-called burrs (excess paraffin attached to the paraffin block) occur in the paraffin block. Deburring is not necessary.

In this invention, it shows an example of the tray which standingly arranged the columnar body, (a) is sectional drawing, (b) is a top view, (c) is a principal part expanded sectional view. It is the schematic which shows the example of the cross-sectional shape of a columnar body. It is the schematic which shows the example of the other cross-sectional shape of a columnar body. It is a schematic sectional drawing which shows the other example of the tray which erected the columnar body. (A), (b) is a schematic sectional drawing which shows the example which stood the columnar body in the bottom part back surface of the cassette main body, respectively. It is a schematic sectional drawing which shows the state which produces a paraffin block using the tray which erected the columnar body of FIG. It is a schematic sectional drawing which shows the paraffin block obtained in FIG. It is a top view which shows the microscope specimen obtained by slicing the paraffin block of FIG. It is a schematic sectional drawing which shows the condition which produces a paraffin block using the cassette main body which installed the columnar body of FIG. 5 (a). It is a schematic sectional drawing which shows the paraffin block obtained in FIG. It is a schematic sectional drawing which shows the microscope specimen obtained by slicing the paraffin block of FIG. It is a schematic sectional drawing which shows the other example of the tray used for this invention. It is a perspective view which shows an example of the cassette for medical examinations. It is a schematic sectional drawing which shows the state which accommodated the cassette sample for medical examinations of FIG. It is a perspective view which shows an example of the conventional tray. It is a schematic sectional drawing which shows the state which produces a paraffin block using the tray of FIG. It is a schematic sectional drawing which shows the paraffin block obtained in FIG. It is a top view which shows the microscope specimen obtained by slicing the paraffin block of FIG.

The paraffin block creating tool of the present invention comprises a medical examination cassette comprising a cassette body and a lid, a paraffin housing part and a cassette body mounting part provided at the periphery thereof, and a paraffin in which a specimen is embedded with paraffin. It includes a tray for forming a block, a columnar body that does not melt at the liquefaction temperature of the paraffin and can be sliced, and a means for standing the columnar body in a paraffin container of the tray. .
The cassette used in the present invention includes a conventional cassette including a cassette body 12 and a lid 13, and typical examples thereof include the cassettes shown in FIGS. 13 and 14.
In the present invention, a columnar body that does not melt at the liquefaction temperature of paraffin and can be sliced is erected in the paraffin container of the tray.
FIGS. 1A, 1 </ b> B, and 1 </ b> C show an example of the tray 1 in which the columnar body 5 is erected by the support portion 6 provided in the paraffin storage portion 2. As shown in these drawings, a columnar body 5 is supported as a means for erecting a paraffin container 2 of a tray 1 composed of a container-shaped paraffin container 2 and a cassette mounting part 3 provided at the periphery thereof. Part 6 is included. In the figure, reference numeral 4 denotes a frame portion that also serves as a gripping portion.

  In the present invention, the columnar body 5 is not liquefied at the liquefaction temperature of paraffin (about 60 ° C.) and can be sliced, and is identifiably colored or has an identifiable cross-sectional shape. Examples include crayon, crepas, colored paraffin, rubber, elastomer, soft resin, and konjac. In particular, since the crayon and the crepa are colored in 12 colors, 24 colors, etc., these colors can be used as an identification display.

  The colored paraffin is not particularly limited, and all paraffins usually used for preparing a paraffin block and a predetermined dye or pigment can be suitably used. In addition, since white paraffin is normally used for the paraffin block B, it is easier to see the identification display 5a if the contrast of the colored liquid paraffin B is higher than that of the white paraffin. For this reason, it is preferable to select the dye and the pigment so that the lightness is low and the saturation is high.

  By the way, when the melting point of the paraffin used as the columnar body is low, the columnar body may become too soft and deform when the high temperature molten paraffin is poured into the tray. In order to avoid such inconvenience, it is preferable to use a paraffin used for the colored paraffin having a melting point comparable to or slightly higher than that of the paraffin used for the paraffin block main body, more preferably 5 ° C. or higher. Is used.

  The rubber is not particularly limited and may be either natural rubber or synthetic rubber. Examples of the synthetic rubber include butadiene rubber (BR), styrene-butadiene rubber (SBR), chloroprene rubber (CR), butadiene-acrylonitrile rubber ( NBR), ethylene-propylene rubber (EPM, EPDM), silicone rubber and the like. Examples of the elastomer include styrene-based, olefin-based, ester-based, and urethane-based thermoplastic elastomers. Examples of the soft resin include polyethylene and soft vinyl chloride resin. Rubber, elastomer, soft resin, and konjac can also be colored, and these colors can be used as an identification display.

Moreover, the columnar body 5 can use these cross-sectional shapes as an identification display as cross-sectional shapes which can be identified.
In FIG. 1, a square is shown as a cross-sectional shape, but the cross-sectional shape is not limited thereto, and any cross-sectional shape that can be identified (differentiated) may be used. For example, as shown in FIG. 2 and FIG. 3, symbols such as ◯, Δ, and X, alphabets such as A, B, and C (a, b, c), a, b, c (a, b, c), etc. Katakana, Hiragana, 1, 2, 3, etc., such as stars, hearts, diamonds, clovers, etc., letters, numbers, symbols, patterns and the like.
Furthermore, it is also possible to display the identification by combining the color and the cross-sectional shape.

  When the liquid paraffin is poured into the paraffin container 2 of the tray 1 and the tray 1 is removed after the liquid paraffin has solidified, the columnar body 5 remains in the obtained paraffin block and remains in the color of the columnar body 5. Or the identification display which consists of a cross-sectional shape or both is formed.

The columnar body 5 is erected in the paraffin accommodating part 2 of the tray 1, and as such erection means, a supporting part provided in the paraffin accommodating part 2 of the tray 1 or the cassette body 12 is preferable.
As for the former, for example, as shown in FIG. 1, the support portion 6 (6 a) protruding from the bottom rear surface of the tray 1, or as shown in FIG. 4, the support portion 6 (6 b) protruding from the bottom surface. It is good. In this case, you may provide the latching claw 7 for latching the columnar body 5 inside the support part 6 (6a) (refer FIG.1 (c)). Similarly, the locking claw 7 can be provided in the support portion 6 (6b) of FIG.
As for the latter, for example, as shown in FIG. 5, a support portion 6 can be provided on the bottom rear surface of the cassette body 12. 5A shows an example of a support portion 6 (6c) projecting inward of the cassette body 12 on the bottom rear surface, and FIG. 5B shows a support portion 6 projecting outward on the bottom rear surface. An example of (6d) is shown. The locking claws 7 described above can also be provided in these support portions 6 (6c) and 6 (6d).
Further, as another standing means, the paraffin container 2 of the tray 1 or a piercing protrusion provided on the bottom rear surface of the cassette body 12 can be used as a supporting part, and the columnar body 5 can be pierced and erected. It is.

  When the cassette 11 (cassette body 12) is placed on the cassette placement portion 3 of the tray 1, the columnar body 5 is located between the bottom rear surface of the cassette 11 (cassette body 12) and the bottom surface of the tray 1. It is set up so that it may fit in. Further, the tray 1 is removed after the liquid paraffin is solidified, and at this time, the columnar body 5 is preferably formed in a tapered shape so as to remain in the solidified paraffin (FIGS. 1A and 4). FIG. 5 (a) and FIG. 5 (b)).

  The tray 1 includes a paraffin container 2 made of a container having a square shape and the like, and a cassette mounting portion 3 provided on the periphery thereof, and a frame portion 4 is provided as necessary. Examples of the material of the tray 1 include metals such as stainless steel, copper, and aluminum, ABS resins, AS resins, polystyrene resins, polymethyl (meth) acrylate resins, polyacetal resins, polyamide resins, polyester resins, and polycarbonate resins.

FIG. 6 shows a state in which a cassette block is created using the tray 1 in which the columnar body 5 shown in FIG. 1 is erected.
That is, in the same manner as described in the background art, the specimen S that has been washed with water, treated with a chemical solution, and the like is transferred from the cassette body 12 to the paraffin container 2 of the tray 1 and is transferred to the cassette mounting portion 3 of the tray 1. 12 is poured and liquid paraffin P is poured to the upper part of the cassette body 12.

  Next, as shown in FIG. 7, after the liquid paraffin P is solidified, the tray 1 is removed, but the columnar body 5 remains in the solidified paraffin P. As a result, the specimen S is embedded, and the columnar body 5 is removed. A paraffin block 8 containing is obtained.

  Next, as described in the background art, the paraffin block 8 is turned upside down and fixed on the microtome, and the portion of the paraffin P including the columnar body 5 embedded in the sample S is sliced as shown in FIG. A thin piece in which the identification display 5a having the cross-sectional shape of the columnar body 5 is embedded is obtained, and a microscope specimen H is obtained by processing such as staining.

  In the obtained microscope specimen H, the specimen S and the identification display 5a are not separated from each other and are not separated from each other, so that they cannot be mistaken for other microscope specimens.

FIG. 9 shows a state in which a cassette block is created by using the cassette 11 (cassette body 12) in which the columnar body 5 is erected as shown in FIG.
FIG. 10 shows the state in which the tray 1 is removed after the liquid paraffin P is solidified, the columnar body 5 remains in the solidified paraffin P, the specimen S is embedded, and the paraffin block 8 including the columnar body 5 is obtained. FIG. 11 is a microscopic specimen H obtained by slicing from the paraffin block 8 and embeds the specimen S and the identification display 5a.

  By the way, the tray 1 has the cassette mounting portion 3 and the frame portion 4 at the upper edge in a horizontal shape, and as shown in FIG. 6, the paraffin P is solidified in a state where the horizontal portion is raised. As shown in FIG. 7, this portion becomes a burr 9 and remains in the paraffin block 8. The burr 9 has a problem when the paraffin block 8 is engaged with the adapter and fixed on the microtome, so it needs to be scraped off with a cutter or the like, and the workability is lowered.

  In order to prevent the occurrence of such burrs 9, as shown in FIG. 12, the tray that has the upper edge portion of the tray 1 erected obliquely or vertically and used as the cassette mounting portion 3 has the occurrence of burrs 9. This is preferred because there are no horizontal parts to cause. In this case, the cassette main body 12 may be simply placed on the cassette placement portion 3 which is the upper edge of the tray 1, but as shown in the figure, the cassette placement portion of the tray 1 is placed behind the bottom of the cassette main body 12. 3 is used, the liquid paraffin is prevented from flowing out to the outside by the groove of the cassette body 12 fitted and the cassette mounting portion 3 of the tray 1. Is prevented from occurring.

  As described above, according to the paraffin block preparation tool of the present invention, a microscope specimen including an identification display that is inseparable from the specimen can be obtained, so that serious troubles such as mistaking the microscope specimen are prevented, and the specimen reliability is improved. To do.

DESCRIPTION OF SYMBOLS 1 Tray 2 Paraffin accommodating part 3 Cassette mounting part 4 Frame part 5 Columnar body 5a Identification display 6 (6a), (6b), (6c), (6d) Support part 7 Locking claw 8 Paraffin block 9 Burr S Sample H Microscope specimen 11 Medical examination cassette 12 Cassette body 12a Recording section 13 Cover 14 Through hole 15 Locking section T Conventional tray T1 Frame section T2 Cassette mounting section P Paraffin B Paraffin block

Claims (6)

A medical examination cassette comprising a cassette body and a lid;
A tray for creating a paraffin block in which a specimen is embedded in paraffin, comprising a paraffin storage part and a cassette body mounting part provided at the periphery thereof;
A columnar body that does not melt at the liquefaction temperature of the paraffin and can be sliced;
Means for erecting the columnar body in the paraffin container of the tray,
The paraffin block creating tool, wherein the means for standing the columnar body in the paraffin container is a support provided at the bottom of the tray. The paraffin block making tool according to claim 1 , wherein the columnar body is selected from crayon, crepas, colored paraffin, rubber, elastomer, soft resin, and konjac. 3. The paraffin block making tool according to claim 2 , wherein the columnar body is made of colored paraffin and has a melting point higher than that of the paraffin of the paraffin block body. The paraffin block making tool according to any one of claims 1 to 3 , wherein the columnar body has a cross-sectional shape that can be identified. The paraffin block creating tool according to claim 4 , wherein the identifiable cross-sectional shape is selected from letters, numbers, symbols, and patterns. The paraffin block creation tool according to any one of claims 1 to 5 , wherein an upper edge portion of the tray is erected in an oblique or vertical direction.

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