JP2018136128A - Paraffin block and paraffin block preparation method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a paraffin block which eliminates seams of paraffin embedding a specimen and does not peel off from the seams at the time of thinning or breaks and a paraffin block preparation method.SOLUTION: A paraffin block having a specimen S embedded in a paraffin P attached to a bottom surface portion of a cassette body C1 is characterized by a seamless molding of the paraffin P including the specimen S. A method of preparing a paraffin block having the specimen S embedded in the paraffin P attached to the bottom surface portion of the cassette body C1 is characterized by sticking a double-sided adhesive tape 2 on the bottom surface of a tray T, sticking the sample S which has been subjected to water washing and chemical treatment on the double-sided adhesive tape 2, placing the cassette body C1 on the tray T and pouring liquid paraffin P until the cassette body C1 is immersed.SELECTED DRAWING: Figure 5

Description

  The present invention relates to a paraffin block and a method for producing a paraffin block. More particularly, the present invention relates to a paraffin block and a method for producing a paraffin block that eliminates a seam of paraffin and does not peel or break from the seam when slicing.

  Conventionally, a cassette for medical examination (hereinafter sometimes simply referred to as a cassette) used to create this type of microscope specimen is a cassette body C1 made of a chemical-resistant synthetic resin, as shown in FIGS. And a lid C2. The cassette body C1 is a rectangular container having an open top surface, and has a plurality of through holes C3 on the bottom surface, and is a plate-shaped recording portion that is inclined outwardly toward the bottom surface on the outer side of one side wall on the short side. C1a is provided. The lid C2 is a detachable plate-like body and has a large number of through holes C3 on the plate surface.

  In order to prepare a microscope specimen using the cassette C, first, as shown in FIG. 8, the collected specimen S is accommodated in the cassette body C1, the lid C2 is attached, and the subject is attached to the recording section C1a. Record names, symbols, etc. to identify

  Subsequently, the specimen S is washed with water through the through-hole C3 of the lid C2, the moisture of the specimen S is removed with alcohol, the fat content of the specimen S is removed with xylene, and affinity with liquid paraffin described later is imparted. .

  Next, as shown in FIGS. 9 and 10, liquid paraffin P is put in a stainless steel tray T, and the specimen S is taken out from the cassette body C 1 and transferred to the tray T. A small amount of paraffin P (P1) is added, and liquid paraffin is infiltrated into the specimen S to temporarily fix the specimen S. Subsequently, as shown in FIG. 11, the cassette body C1 is placed on the cassette mounting portion T2 of the tray T, and additional liquid paraffin P (P2) is poured onto the sample S until the cassette body C1 is immersed.

  Next, as shown in FIG. 12, by removing the tray T after the liquid paraffin P (P1, P2) is solidified, the paraffin P (P1, P2) in which the specimen S is embedded adheres to the bottom surface of the cassette body C1. Paraffin block B is obtained.

  Next, as shown in FIG. 13, by placing the paraffin block B upside down on a microtome (not shown) and engaging the adapter installed in the microtome with the paraffin block B, the paraffin block B is converted into the microtome. Fix it. Subsequently, a portion in which the specimen S of paraffin P is embedded is sliced, and the obtained thin piece is subjected to staining and other predetermined processing to obtain a microscope specimen H as shown in FIG.

Japanese Patent No. 4402265

  However, in the above method, it becomes inevitable that there are two layers of the paraffin P1 used for the first infiltration and fixation for embedding the specimen S and the additional paraffin P2, and a seam PJ is generated between the two layers (see FIG. 13). For this reason, when the obtained paraffin block B is fixed to a microtome and sliced, troubles such as cracking or peeling off from the seam may occur, and valuable specimens may be spoiled.

  As a result of diligent research to solve the problems of the prior art as described above, the paraffin embedding the specimen has no seam, and therefore, the problem of cracking or peeling when slicing has been solved. It came to provide the manufacturing method of a paraffin block and a paraffin block.

  That is, a feature of the present invention is a paraffin block in which paraffin embedded with a specimen is attached to the bottom surface of a cassette body, and the paraffin containing the specimen has no seam.

  Another feature of the present invention is a paraffin block characterized in that the specimen has a strip shape.

  Another feature of the present invention is a paraffin block characterized in that the specimen is a minute specimen.

Another feature of the present invention is a method for producing a paraffin block in which paraffin embedded with a specimen is attached to the bottom surface of a cassette body.
Adhere double-sided adhesive tape on the bottom of the tray,
A specimen washed with water and treated with a chemical solution is attached onto the double-sided adhesive tape,
Place the cassette body on the tray,
A paraffin block is produced by pouring liquid paraffin until the cassette body is immersed.

  Another feature of the present invention is a method for producing a paraffin block, characterized in that the specimen has a strip shape.

  Another feature of the present invention is a method for producing a paraffin block, wherein the specimen is a micro specimen.

  The paraffin block of the present invention has a single layer of paraffin that embeds the specimen and has no seam, so there is no trouble like cracking or peeling from the seam when slicing, and valuable specimens are wasted. A microscope specimen can be produced without doing so.

  Further, the method for producing a paraffin block of the present invention is such that liquid paraffin can be poured all at once by sticking a double-sided adhesive tape on the bottom of the tray and sticking a specimen on this tape. Compared with the conventional method in which liquid paraffin is poured in two steps, in which liquid paraffin is poured and then liquid specimen is fixed, and then additional liquid paraffin is poured, the paraffin block can be easily and efficiently produced. .

It is the schematic which shows the paraffin block of this invention. It is the schematic which shows the flaky microscope sample obtained by slicing the paraffin block of FIG. 1 with a microtome. It is the schematic which shows the state which stuck the double-sided adhesive tape on the bottom face of the tray. In FIG. 3, it is the schematic which shows the state which stuck the test substance on the double-sided adhesive tape. It is the schematic which shows the state which mounted the cassette main body on the tray of FIG. 4, and poured the liquid paraffin until the cassette was immersed. In FIG. 5, it is the schematic which shows the state which removed the tray and obtained the paraffin block. It is the schematic which shows an example of the cassette for medical examinations. It is the schematic which shows the state which accommodated the test substance in the cassette of FIG. It is the schematic which shows an example of a tray. It is the schematic which shows the state which poured the small amount liquid paraffin to the tray and infiltrated the paraffin to the test substance, and was fixed on the bottom face of the tray. It is the schematic which shows the state which mounted the cassette main body on the tray of FIG. 10, and poured the liquid paraffin until the cassette main body was immersed. In FIG. 11, it is the schematic which shows the state which removed the tray and obtained the paraffin block. It is the schematic which shows the state sliced with the microtome by turning the paraffin block of FIG. 12 upside down. It is the schematic which shows the flaky microscope sample obtained by slicing.

The paraffin block of the present invention is characterized in that the paraffin block including the specimen is seamless in the paraffin block in which the paraffin embedded with the specimen is attached to the bottom surface of the cassette body.
In addition, the method for producing a paraffin block of the present invention is a method for producing a paraffin block in which a paraffin embedded with a specimen is attached to the bottom surface of a cassette body, and a double-sided adhesive tape is attached to the bottom surface of the tray, The specimen washed with water and treated with a chemical solution is stuck on the double-sided adhesive tape, the cassette body is placed on a tray, and liquid paraffin is poured until the cassette body is immersed.

FIG. 1 shows a paraffin block according to the present invention, and shows a state of slicing with a microtome (not shown) (the specimen side is the upper part and the cassette body side is the lower part). As shown in FIG. 1, the paraffin block 1 of the present invention is characterized in that the paraffin P embedded with the specimen S is attached to the bottom surface of the cassette body C1, and the paraffin P is composed of one layer and there is no seam. It is. In FIG. 1, the burrs protruding from the cassette body C1 are cut off.
Therefore, as shown in FIG. 2, when slicing the paraffin block 1 and obtaining a flaky microscope specimen H composed of the specimen S embedded in the paraffin P, the paraffin P is broken, Troubles such as peeling do not occur.

Regarding the method for producing the paraffin block of the present invention, first, as shown in FIG. 3, the double-sided pressure-sensitive adhesive tape 2 is stuck on the bottom surface of the specimen storage portion T3 of the tray T.
Next, as shown in FIG. 4, the specimen S that has been washed with water and treated with a chemical solution is attached onto the adhesive tape 2. This washing with water and chemical treatment are the same as in the prior art, and are intended to give the specimen S affinity for liquid paraffin.

  Next, as shown in FIG. 5, the cassette body C1 is placed on the cassette placing portion T2 of the tray T, and liquid paraffin is poured until the cassette body C1 is immersed. At this time, since the sample S is stuck on the bottom surface of the sample container T3 of the tray T via the double-sided adhesive tape 2, the sample S does not float even when liquid paraffin P is poured.

  The purpose of pouring liquid paraffin until the cassette body C1 is immersed is to sufficiently attach the paraffin embedded with the specimen S in the tray T to the bottom of the cassette body C1 through the through hole C3 in the bottom of the cassette body C1. This is to prevent separation from the bottom of the cassette body C1 when slicing with a microtome later. Therefore, it is usually poured so as to be about 80 to 100% of the height of the cassette body C1, but usually it is cooled and contracted to about 75 to 95%.

Next, as shown in FIG. 6, the paraffin block 1 is obtained by removing the tray T. At this time, the double-sided pressure-sensitive adhesive tape 2 is attached to the tray T and removed together with the tray T.
The obtained paraffin block 1 is a liquid paraffin used for imparting affinity before being attached onto the double-sided adhesive tape 2, and the liquid paraffin poured until the cassette body C1 is immersed is double-sided adhesive with the specimen S. A thin layer of paraffin is formed on the surface of the specimen S because it goes around the tape 2. In FIG. 6, the burrs protruding from the cassette body C1 are cut off.

  As shown in FIG. 1, the obtained paraffin block is sliced upside down, that is, in a state in which the specimen S side is at the top and the cassette body C1 side is at the bottom and engaged and fixed to the adapter on the microtome. As a result, a microscope specimen as shown in FIG. 2 is obtained.

  The specimen in the present invention is not particularly limited as long as it is attached to the double-sided pressure-sensitive adhesive tape, but a strip-like specimen is preferable from the viewpoint of attachment workability. In addition, even a minute specimen such as a polyp can be easily attached and fixed to a double-sided adhesive tape, so that the workability is greatly improved compared to the case of fixing with paraffin as in the prior art. Furthermore, since a plurality of microscopic specimens can be arbitrarily stuck and fixed on the double-sided adhesive tape randomly or regularly, a plurality of specimens can be processed at once.

The medical examination cassette in the present invention is not particularly limited as long as it is composed of a cassette body and a lid. In the above description, a cassette having an inclined plate-like recording portion is exemplified. A cassette that does not have a recording section can also be used.
Further, the tray in the present invention is not particularly limited, and the tray having the frame portion is exemplified in the above, but a tray having no frame portion can also be used.
Moreover, the thing of the shape which made asymmetrical either the left or right of a sample storage part can also be used so that the left-right direction of a microscope sample can be visually recognized.

As mentioned above, the paraffin block of the present invention consists of a single layer of paraffin embedded with a specimen and has no seam. Therefore, when a sliced microscopic specimen is prepared by slicing, the paraffin block is cracked or peeled off. Troubles such as ruining valuable specimens are prevented.
Further, the method for producing a paraffin block of the present invention is such that liquid paraffin can be poured all at once by sticking a double-sided adhesive tape on the bottom of the tray and sticking a specimen on this tape. Compared with the conventional method in which liquid paraffin is poured in two steps, in which liquid paraffin is poured and then liquid specimen is fixed, and then additional liquid paraffin is poured, the paraffin block can be easily and efficiently produced. .

DESCRIPTION OF SYMBOLS 1 Paraffin block 2 Double-sided adhesive tape C1 Cassette main body C1a Recording part C2 Cover C3 Through-hole T Tray T1 Frame part T2 Cassette main body mounting part T3 Specimen storage part P, P1, P2 Paraffin PJ Paraffin joint B Paraffin block

Claims (6)

  A paraffin block in which a paraffin embedded with a specimen is attached to a bottom surface of a cassette body, wherein the paraffin containing the specimen has no seam.   The paraffin block according to claim 1, wherein the specimen has a strip shape.   The paraffin block according to claim 1, wherein the specimen is a minute specimen. In the method of producing a paraffin block in which the paraffin embedded with the specimen is attached to the bottom surface of the cassette body,
Adhere double-sided adhesive tape on the bottom of the tray,
A specimen washed with water and treated with a chemical solution is attached onto the double-sided adhesive tape,
Place the cassette body on the tray,
A method for producing a paraffin block, wherein liquid paraffin is poured until the cassette body is immersed.   The method for producing a paraffin block according to claim 4, wherein the specimen has a strip shape.   The method for producing a paraffin block according to claim 4, wherein the sample is a micro sample.

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