JP4058709B2 - Pathological tissue sample cassette and method for forming an embedding block using the same - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
In the present invention, since a clinician decides a diagnosis and treatment of a disease, a pathological tissue sample collected from a human body of a patient is converted into a thin-stained specimen, and the pathological tissue sample is managed until it is returned to the clinician. The present invention relates to a cassette for a pathological tissue sample used in a system and a method for forming an embedding block used therein.
[0002]
Further, when forming an embedded block of a pathological tissue sample in a state before slicing the pathological tissue sample, the burrs are not generated in the embedded block, the thickness at the time of slicing is made uniform, and a pathological examination is performed. In the conventional embedding process, burrs are generated around the conventional cassette due to its structure, and the burrs are eliminated by a knife or heat. The deburring operation process is eliminated and streamlined, and the sample data printed on the periphery of the cassette and damaged during the deburring operation by the conventional cassette and embedding method is used to prepare the sample data after the embedding block is prepared. This makes it difficult to read the data.
[0003]
Furthermore, the clinician collects the patient's sample from each patient, transfers the collected sample to a pathological examination room, etc., and then cuts out from the reception / entry on the pathological examination side, fixing / dehydrating / degreasing・ Medical treatment for penetration of penetrating embedding agent, embedding work, slice cutting, section scooping, section extending and pasting work, sealing and storage management work for sliced block specimens, specimen staining, encapsulation arrangement arrangement work, pathological diagnosis Each patient sample from the completion of the verification check of each patient's slide specimen and request form data and the input work of diagnostic data, the file and search work of each patient's past history data to the dispatch work of the diagnostic data, etc. Barcode and two-dimensional suitable for each work management system that enables reliable storage and tracking of each patient sample (with attached request information, report to be written, and history data) Paste the chromatography data code label or the like, a related method of forming the embedded block using the same, and pathologic tissue samples cassette can be observed confirmation from the cassette outside the interior of the sample.
[0004]
Still further, the present invention relates to a pathological tissue sample cassette used in a system for tracking and confirming each pathological tissue management of each patient after each stage and a method for forming an embedding block using the cassette.
[0005]
[Prior art]
Conventionally, in this type of work, a pathological tissue sample collected from each patient by a clinician is stored in a container with a label on which the name of each patient or other data is written, and the like. Together with the inspection request document describing the attributes and clinical diagnosis and findings, these are transferred by appropriate means such as mail or messenger.
[0006]
Acceptance and entry of patient specimens before specimen preparation work for pathologists to diagnose specimen specimens of each patient stored in the container above, which has been transported to the pathological examination site away from the clinician Check the pathological diagnosis request form that is sometimes attached together with the patient sample container, the name of each patient, the contents of the request, the status of the pathological tissue sample, etc., and the pathological tissue sample belongs to the patient Make sure.
[0007]
After that, at the time of acceptance / entry, it is related to the patient ID number and medical record number of each patient at the time of collecting the pathological tissue sample of the clinician and the pathological tissue sample collating random number number at the time of receiving the patient sample collection / delivery person or messenger sample Do not store the pathological tissue sample or the biopsy pathological tissue sample of the patient that has been trimmed in another cassette (processing rod) in which the patient sample number is newly described with the serial specification number or the sample number, and then the specimen Process the chemicals for preparation, pick all the samples with tweezers, transfer them to the bottom of the embedding dish, embed them with the embedding agent, attach them to the rootstock (base), and solidify the embedding agent A block sample is prepared by scraping and removing excess burrs around the embedding block to be formed later.
[0008]
This block sample is sliced, stretched and pasted on a slide glass, dried, decapsulated (deparaffinized), and stained and sealed to prepare a stained slide specimen, which is then passed to each patient by a pathologist. The pathological diagnosis is performed by collating the specimen sample, the request form data and the past history data, and a pathological diagnosis report is made to the clinician.
[0009]
In the above-mentioned process, each pathological tissue sample is lost between the time when the clinician samples the pathological tissue sample data (collected sample, request form data and past history data) of each patient until the completion of the pathological diagnosis report. Since inspections and checks to prevent contamination, mixing, and replacement are performed by discontinuous human resources, the location of the responsibility of the worker at each work stage is also unclear after the fact.
[0010]
[Problems to be solved by the invention]
The object of the present invention is to collect specimen sample data (collection) of each patient in the work from the time of collection of a histopathological specimen sample including a biopsy sample collected from each patient of the clinician until the completion of the pathological diagnosis report. The risk of loss of sample, request form data, and past history data) due to loss or mixing, and human error in replacement is eliminated, and if such an error occurs, where the trouble occurred after that Clarify and clarify where the responsibilities are.
[0011]
Also, in the above-mentioned work process, human work can be shortened or omitted to eliminate troubles that become a major problem that cannot be recovered by simple human mistakes, and management and monitoring by automation and mechanization can be performed. It is to increase the work process.
[0012]
  Because of that, alreadyThis caseInventor(Sometimes called "inventor")ByPatentapplication(Sometimes referred to as “invention application”)Macro images that can be used to observe and confirm the pathological tissue samples stored in the perforated dish from the outside of the fixed transfer container or through the cassette window, and those samples were described and attached at the time of collection on the clinical side Patient identification data (patient attributes, patient ID, patient chart number, etc.) and the path number on the pathology side, sampling from patients on the clinical side, description of pathological diagnosis examination request data including clinical data and pathology In all work processes from the time of submitting a diagnosis request to receiving, transporting, receiving, sorting, chemical processing, embedding, specimen preparation, history search, pathological diagnosis, data management storage, and pathological report issuance It is to be used in a unified manner.
[0013]
To that end, in addition to the observation and confirmation of the macro image of the sample in the cassette, means such as a sample data number, bar code, two-dimensional data code, etc., for associating and associating each patient data with the cassette sample are necessary. In the conventional embedded block manufacturing method, the data description surface was damaged during the deburring operation of the cassette substrate, and automatic mechanical reading could not be performed, so patient data from the clinical side could not be directly introduced to the pathological specimen preparation process side. I was in It is to protect the description surface of the block sample data which is also the patient sample data.
[0014]
[Means for Solving the Problems]
The cassette for small pathological tissue samples of the present invention is a frame-shaped substrate in which an opening is provided on the upper and lower sides, and a porous support bottom plate with a window is formed on the lower side, and the inventor in the frame-shaped substrate has already applied for an invention. Patent No. 3049537, Japanese Patent Application No. 7-300290 and Japanese Patent No. 3008078, and a porous plate-shaped lid or a window-shaped porous plate-shaped lid fitted into the upper opening of the frame-shaped base And a support for the perforated plate protruding on the upper surface of the dish-shaped porous bottom plate that is detachably fitted to the window portion of the lower porous support bottom plate with a window, and the porous support bottom plate with the same lower window A device for embedding a block of a pathological tissue sample, which comprises an embedding plate that is fitted to the outer periphery of the cassette for biopsy small pathological tissue sample and the outer peripheral surface of the embedding plate almost coincides with the outer peripheral surface of the embedding plate In the inner circumference of the window portion of the porous support bottom plate with a window on the lower side An inwardly projecting support body that can be fitted and inserted into the dish-shaped porous bottom plate is formed on the dish-shaped porous bottom plate, and the inner peripheral surface of the dish-shaped porous bottom plate and the inner peripheral surface of the embedding dish are alternatively selected from the frame. And watertight fitting to the outer peripheral surface of the porous support bottom plate with a window protruding in a smaller area than the outer peripheral surface of the bottom surface of the substrate.
[0015]
Further, the cassette for a large pathological tissue sample or fragment sample has a frame-like base body having an opening on the upper and lower sides and a porous support bottom plate on the lower side, and a porous fitting fitted into the upper opening of the frame-like base. A pathological tissue sample embedding block forming instrument comprising a plate-shaped lid and a dish-shaped porous bottom plate having no support that is detachably fitted to the lower window opening, and the inner periphery of the dish-shaped porous bottom plate A surface and an inner peripheral surface of the embedding dish alternatively water-tightly fitted to the outer peripheral surface of the porous support bottom plate projecting with an area smaller than the outer peripheral surface of the bottom surface of the frame-shaped substrate, and the pathological tissue sample The outer peripheral surface of the frame-shaped substrate of the cassette for use and the outer peripheral surface of the embedding dish almost coincide with each other. At this time, for the dish-shaped porous bottom plate for fragment samples that does not maintain a firm shape, a dish-shaped porous bottom plate having a smaller pore diameter than the porous portion of the dish-shaped porous bottom plate used for other large-sized pathological tissue samples is used. Is desirable.
[0016]
Also, a sample data description surface protection grid plate that protects the data description surface such as the sample number, bar code, and two-dimensional data code printed or pasted on the block sample data description location of the cassette from the cassette lid In the conventional embedding molding method, it is inevitably overmolded around the frame base of the block sample cassette, and when the block sample is sliced, the block sample is securely placed in the holder of the microtome adapter. A pathological tissue sample comprising a plate-like porous bottom plate having no support that is detachably fitted to the lower window opening so as not to cause burrs that hinder horizontal holding in the embedding process. In the embedding block forming instrument, the inner peripheral surface of the dish-shaped porous bottom plate and the inner peripheral surface of the embedding dish are alternatively selected from the outer peripheral surface of the bottom surface of the frame-shaped substrate. The outer peripheral surface and the outer peripheral surface of the embedding dish of the frame-shaped substrate watertight fitted and the pathology tissue samples cassette on the outer peripheral surface of the porous support bottom plate which projects with no space and is one that most matches. By changing the porous inner diameter of the dish-shaped porous bottom plate, the porous inner diameter of the porous support bottom plate, which is the bottom of the cassette, can be kept constant without changing depending on the size of the sample. In the embedding agent below the bottom of the cassette when embedding an embedding block in which an embedding agent such as paraffin is poured into an embedding dish that is watertightly fitted to the outer peripheral surface of the porous support bottom plate protruding in a smaller area than the bottom outer peripheral surface of the base Since bubbles are not formed and burrs are not generated, the deburring process is omitted and damage to the block sample description surface that occurs in this process is prevented.
[0017]
DETAILED DESCRIPTION OF THE INVENTION
  As shown in FIGS. 1 to 3, an opening 2 is formed on the upper side of the frame-shaped substrate 1, and a porous support bottom plate 3 with a window 3 c is formed on the lower side, and one end of the porous plate-like lid 4 with respect to the opening 2 is formed. The locking piece 4a is locked to the locking protrusion 1a on one side of the frame-shaped substrate 1, and the locking projection piece 4b on the front side of the inclined sample data describing surface protection grid plate 4c on the other end of the lid 4 is fixed to the frame-shaped substrate 1. The porous plate-like lid 4 is detachably attached to the opening 2 of the frame-shaped substrate 1 by engaging with the locking groove 1c on the upper side of the sample data description location 1b of the frame-shaped substrate 1 formed on the other side. The outer peripheral surface 3a of the porous support bottom plate 3 with the window 3c is a frame-shaped base.body1 is protruded further downward than the lower end surface 1d.
[0018]
The inner peripheral surface 5a of the dish-shaped porous bottom plate 5 is fitted in a watertight and detachable manner on the outer peripheral surface 3a of the porous support bottom plate 3 with the window 3c.
At this time, a locking recess 3b is formed on the outer peripheral surface 3a, and a locking projection 5b formed on the inner peripheral surface 5a is detachably fitted to the locking recess 3b. It is desirable that the end surface 1d and the upper end surface 5d of the dish-shaped porous bottom plate 5 and the lower end outer peripheral surface 3e of the porous support bottom plate 3 and the inner peripheral surface 5c of the dish-shaped porous bottom plate 5 are in close contact with each other.
[0019]
Further, instead of fitting the plate-shaped porous bottom plate 5 to the outer peripheral surface 3a of the porous support bottom plate 3 with the window 3c, the inner peripheral surface 6a of the embedding plate 6 used when embedding the biopsy sample is selected. When fitting, the inner peripheral surface 6a of the embedding dish 6 is detachably and watertightly fitted to the outer peripheral surface 3a, and the outer peripheral surface 1d and lower end surface 1e of the frame-like base and the embedding dish The outer peripheral surface 6b of 6 and the upper end surface 6e of the embedding dish almost coincide with each other.
[0020]
7 is a perforated dish with a lid used for accommodating a biopsy sample of a relatively small size (Japanese Patent No. 3049537 and Japanese Patent Application No. 7-3000290 and Japanese Patent No. 3008078 already filed by the inventor). 7a is a lid.
The surrounding flange portion 7j is sandwiched and held by a support 5f protruding from the lower surface of the lid 4 and the upper surface of the dish-shaped porous bottom plate 5.
[0021]
The biopsy sample cassette of the present invention has the above-described structure, and is used by the clinician to be stored in a predetermined biopsy storage section 7d of the lid-coated perforated plate and transported to a pathologist by appropriate transport means. When making the embedding block 10 using the biopsy sample 8 in the perforated plate 7 with a lid, first, as shown in FIGS. 3 to 6, the biopsy sample 8 submitted by a clinician is used as the support. By fitting the dish-shaped porous bottom plate 5 on which the projection 5f is provided to the porous support bottom plate 3 with the window 3c, the support 5f is also provided on the inside of the bottom of the frame-shaped substrate. The biopsy sample 8 and the perforated plate 7 with a lid are picked with tweezers 9 and transferred into the frame-like base 1 to be fitted. As shown in FIG. 5, the frame-like base 1 and the perforated plate with a lid are placed on the plate-like perforated bottom plate 5. 7 and the lid 4 are assembled in sequence. Thereafter, a chemical treatment process such as dehydration, degreasing, penetration, and permeation is performed on the pathological tissue sample together with the cassette.
[0022]
Further, in the case of a large pathological tissue sample that does not enter the perforated dish 7, a dish-like perforated bottom plate 5 in which the support 5f is not formed may be used as shown in FIG. At this time, the support 5f is not projected in the frame-shaped substrate, and the maximum volume in the frame-shaped substrate can be widely used, and a trimmed sample obtained by cutting out a large pathological tissue sample is stored in the frame-shaped substrate. can do.
[0023]
Further, as shown in FIG. 4, it is also convenient to use a cassette bottom portion in which the window 3c is not formed on the porous support bottom plate 3 of the frame-like substrate. This is because the surgically-extracted material 8a is not necessarily limited to the one whose shape of the tissue is fixed, but there is also an extractive material (fragment sample) that scrapes the tissue of the affected area. There is a high risk of flowing out of the fluid hole, and it may be necessary to process a larger amount of tissue than the biopsy sample 8. For such a case, as shown in FIG. 16, the cassette bottom portion (frame-like substrate 1) having a large fluid passage hole of the porous support bottom plate 3 without the window 3c is formed, and the kind of tissue sample to be processed with the chemical solution. Depending on the properties of the plate-like substrate 1, the diameter of the liquid-permeable pores of the dish-like porous bottom plate 5 to be fitted to the porous support bottom plate 3 of the frame-like substrate 1 can be selected and used. At this time, a similar result can be expected even if the perforated plate as shown in the right diagram of FIG. 16 is fitted into the bottom surface inside the cassette bottom (frame base 1).
[0024]
When embedding a sample in which the embedding agent has penetrated after completion of the chemical treatment process, in the case of a biopsy sample, as shown in FIGS. A biopsy sample is picked from the support case with tweezers 25 and placed on the inner bottom surface of the embedding dish 6. In the case of other large samples, as shown in FIG. 6, the sample is picked from the frame base 1 with tweezers 25, and the sliced inspection surface is directed downward on the bottom surface of the embedding dish 6 in the same manner as the biopsy sample. Place it flush. Thereafter, molten paraffin 15 is poured into the space 14 formed above and below the porous support bottom plate 3 inside the frame-like substrate 1 and the embedding dish 6 in the state shown in FIG. 6d is held with the thumb and index finger and the embedding dish 6 is not shown, but is transferred onto the cold plate of the embedding device and cooled to embed the biopsy sample 8 together with the porous support bottom plate 3 into a solid state. Then, the tab 6c of the embedding dish is lowered with a finger to remove the embedding dish 6, and the embedding block sample 10 for slicing is formed.
[0025]
At this time, since the outer peripheral surface 3 a of the porous support bottom plate 3 constituting the bottom of the frame-shaped substrate 1 and the inner peripheral surface 6 a of the embedding dish 6 are watertightly fitted, the molten paraffin 15 is placed in the space 14. When it is filled, it does not flow out to the external space 13 through the gap between the fitted portions, and further through the gap between the lower end surface 1d of the frame-shaped substrate 1 and the upper end surface 6e of the embedding dish 6, and the frame shape. Since the outer peripheral surface 1d of the substrate 1 and the outer peripheral surface 6b of the embedding dish 6 are flush with each other, the molten paraffin (embedding agent) 15 attached to the periphery of the frame-shaped substrate 1 is wrapped with the frame-shaped substrate 1. It flows down under the embedding dish 6 almost without staying at the boundary with the embedding dish 6. Therefore, when the paraffin 15 in the gap is cooled to complete the embedding operation and the embedding dish 6 is removed from the embedding block sample 10, a frame that is also a base of the embedding block sample 10. The burr of the solidified paraffin (embedding agent) does not occur in the periphery including the sample data description location 1b of the substrate 1.
[0026]
In addition, depending on the size of the pathological tissue samples 8 to 8a, in the conventional cassette, the size of the inner diameter of the porous 3f must be changed. However, as shown in FIG. The porous support bottom plate 3 of the substrate 1 can be fixed to a constant inner diameter due to the existence of the role if the size of the porous 5h of the dish-shaped porous bottom plate 5 is changed regardless of the presence or absence of the window 3c. Therefore, it is not necessary to make the inner diameter of the porous 3f very small, and the embedding molten paraffin (embedding agent) 15 is placed on the bottom surface of the pathological tissue samples 8 to 8a from the space 14 of the frame base (cassette bottom) 1. It is possible to make it difficult for air bubbles that are likely to be generated when injected into the embedded dish 6. The both sides of the solid embedding block 10 thus formed are sandwiched between the holders 18a and 18b of the microtome adapter 18 shown in FIG. 9, fixed there, sliced with a thin knife 16, and slide glass Paste to form a preparation.
[0027]
At this time, the embedding block 10 formed using the embedding block forming tool of the present invention does not generate burrs of the embedding agent around the embedding block 10, so that it is used as the holder 18 a of the microtome adapter 18. , 18b can be pressed and held accurately, and can be brought into surface contact accurately and stably supported, and then the thickness uniformity and accuracy of the thin section obtained when slicing with a thin cutting knife 16 can be performed. The pathology can be easily examined.
[0028]
Further, since the burrs are not attached to the periphery of the frame-shaped substrate (cassette bottom) 1 which is also a base for the embedded block sample, it is not necessary to remove the burrs, and the burrs are removed from the frame-shaped substrate (cassette bottom) 1. No damage is caused to the surface of the block sample data description portion printed on the periphery of the frame-shaped substrate (cassette bottom portion) 1 that is generated when the sample is removed.
[0029]
Therefore, when the inventor has already applied for a perforated plate, a fixed support case, a fixed fixing support, a fixed transfer container, a biopsy sample storage device, etc., this pathological tissue sample cassette and its embedding plate The pathological tissue samples 8 to 8a collected from the clinical side or excised from the affected part for the purpose of pathological definitive diagnosis and treatment of the patient and the pathological diagnosis request form information of the patient include the patient ID number, attribute, chart number, If patient data such as a patient embossed magnetic card is attached, the pathological diagnosis report will be completed, the accuracy management, data management, and retrieval will be automated and streamlined. It has become possible to utilize the three information of patient data and sample reference number encoded or two-dimensional data.
[0030]
Although the present invention has been described with reference to the above-described embodiment, the present invention is not limited to that embodiment, and specific technical means for more effectively implementing the embodiment or adding the embodiment to the embodiment is described. It is possible to change it partially.
[0031]
For example, if the shape as shown in FIGS. 10 and 16 is taken, the cassette lid 4 and the dish-like porous bottom plate 5 and the embedding dish 6 can be made of stainless steel, not made of synthetic resin, and can be recycled. Also goes up.
[0032]
Further, as shown in FIGS. 13 to 15, a biopsy sample is placed on the bottom surface 7i of the perforated dish 7, and the fixing support agent 23 (Japanese Patent No. 3049537 and Japanese Patent Application No. 11-76843, which the inventor has already applied for an invention). ) Can be fixed and held on the outside or inside of the bottom surface 7i of the bottom 7h of the perforated dish 7. That is, a plurality of or fragile samples can be fixed and held in a single storage section 7d of the cassette or the perforated plate 7 without any separation, so that the subsequent specimen preparation work can be advanced like a single specimen.
[0033]
  At this time, as shown in FIGS.This caseInventor alreadyPatentWhen used in combination with the biopsy sample storage device that has been applied for, biopsy is performed except when the biopsy sample is collected from the patient with a forceps such as an endoscope and when the biopsy sample is sliced with a thin knife. The instrument does not touch the sample directlyofThis eliminates the destruction of biopsy samples other than the two time points that must be touched..
[0034]
Further, as shown in FIG. 13 to FIG. 15, the perforated plate 7 is combined with the fixing support 23, the frame-like substrate 1 provided with the perforated support bottom plate 3 with the window 3c, and the dish-like perforated bottom plate 5 provided with the support 5f. When used, the biopsy sample is surely stored in the cassette inner space surrounded by the bottom 7i of the bottom 7h of the perforated dish 7 and the dish-shaped perforated bottom plate 5, and securely held on the outer bottom of the perforated dish 7. be able to. Thereafter, in the embedding operation after the chemical operation such as dehydration, degreasing, penetration, and infiltration for specimen preparation, the whole cassette is turned upside down as shown in FIG. Instead, the above-mentioned embedding dish 6 is fitted, and it is turned down again and again to the state shown in FIG. 15, and the embedding dish 6 is moved to the cold plate with the holding part 6d and moved to the gel plate. The perforated dish 7 in which the biopsy sample 8 is fixedly held on the bottom surface 7i by the support 24 is pushed down toward the bottom surface of the embedding dish 6 with tweezers 25, and the sample embedding agent (paraffin) 15 is cooled on the bottom surface. If the sample is adhered and held on the bottom surface of the embedding dish 6 due to solidification, the perforated dish 7 is immediately pulled up reversely with the tweezers 25, and only the biopsy sample 8 integrated with the gelation fixing support agent is obtained. It remains on the bottom surface in the embedding dish 6 and the following is the same as in FIG. And, then it is also possible to form the embedded block 10 in the same procedure. That is, in this embedding operation, the tweezers 25 can be embedded without directly touching the sample, and the biopsy sample is not lost, mixed or misplaced when embedded.
[0035]
Further, as shown in FIGS. 17 to 18, the perforated plate and the lid 7a of the case for fixing support which the inventors have already applied for the invention can be used even if separated. The lid part REVERSIBLE LID 7b and the perforated dish bottom part 7h can be fitted with a latching projection 7g of the lid part and the retaining hole 7c of the perforated dish bottom part 7h as shown in FIG. When the sample is stored in a perforated dish and the flange 7j is sandwiched between the support 5f and the lid 4 of the frame-like base (cassette bottom) 1, the locking part between the lid part REVERSIBLE LID 7b and the perforated dish bottom 7h is do not need. Therefore, the REVERSIBLE LID 7b, which is the lid portion, is turned upside down, and the inventor has already applied for the invention as shown in FIG. If the locking hole 4g of the locking projection of the REVERSIBLE LID 7b of the perforated dish 7 having the same diameter and the same distance between the holes as the locking hole 7c of the perforated dish bottom 7h is formed in the cassette, The biopsy sample 8 in the perforated plate sandwiched between the two can be processed without being lost from the predetermined position of the perforated plate 7 during the preparation of the chemical solution for specimen preparation, and the biopsy sample 8 can be observed and confirmed. When opening the lid 4 of the cassette, REVERSIBLE LID 7b, which is the lid portion of the perforated dish, can be opened together with the lid 4, and the biopsy sample 8 of the perforated dish 7 in the cassette can be opened without opening two lids one by one. Can be taken out.
[0036]
【The invention's effect】
As described above, according to the present invention, the outer peripheral surface of the porous support bottom plate constituting the bottom of the frame-shaped substrate and the inner peripheral surface of the embedding dish are water-tightly fitted, and the outer peripheral surface of the frame-shaped substrate and the outer peripheral surface of the embedding dish are Since it fits flush, when the paraffin tissue sample and the porous support bottom plate are embedded integrally by pouring molten paraffin into the space surrounded by the frame-shaped substrate and the embedding dish, the paraffin becomes water tight. It does not flow out to the outside through the fitted part, and does not stay at the boundary between the frame-shaped base and the embedding dish.
[0037]
Therefore, even if the embedding agent (paraffin) is cooled and solidified, the molded embedding block does not generate paraffin burrs. Therefore, the embedding block can be stably supported by the microtome holder. When cutting with a thin sword, the thickness uniformity and accuracy can be improved, the quality of pathological examination can be improved, and there is no deburring work, so there is no deburring work, so the frame-like base that is the base of the embedding block Do not damage the printed surface of the sample data (at the bottom of the cassette).
[0038]
Further, according to the present invention, a sample data description surface protection grid plate is formed to be inclined downward on one side of the lid of the opening of the frame-shaped substrate, and the sample data description surface protection grid plate faces downward on one side of the frame-shaped substrate. Since the upper part of the sample data description part that is inclined to cover the upper part is protected, the barcode, two-dimensional data code, or sample data number etc. printed or affixed to the part of the sample data description part peels off during various operations. Or disappear by various treatment liquids.
[0039]
In addition, the biopsy sample inside the perforated dish stored in the cassette is surely passed through the transparent perforated dish that the inventor has already applied for, through the perforated support bottom plate with the window of the frame-shaped substrate or the window of the perforated plate lid with the window. Observation can be confirmed and it can be captured as a macro image. When this image and the image of pathological examination diagnosis request form data from the clinic and the bar code, two-dimensional data code, sample reference number, etc. are used for patient confirmation and sample tracking management, reliable accuracy control and mechanization with less manpower It helps to advance automation. Therefore, when a human error such as replacement of a biopsy sample occurs, it can be traced even after the fact and the location of responsibility can be clarified.
[Brief description of the drawings]
FIG. 1 is a longitudinal sectional view showing an embodiment of a biopsy sample cassette of the present invention.
FIG. 2 is a longitudinal sectional view of FIG.
FIG. 3 is a partial perspective view of FIG. 1;
FIG. 4 is a perspective view of a portion of FIG. 1 and a portion of another embodiment.
FIG. 5 is a plan view of an embodiment using a part of FIG. 1;
6 is a perspective view showing another embodiment different from the embodiment shown in FIG. 5 in which a part of FIGS. 3 and 4 is combined.
7 is a plan view of a state following the state of FIG.
8 is a longitudinal sectional view showing a state next to the state of FIG.
FIG. 9 is a longitudinal sectional view and a plan view showing another state.
FIG. 10 is a plan view, a front view, a side view, a rear view, and a longitudinal sectional view showing another embodiment.
FIG. 11 is a horizontal and vertical sectional view showing another state.
12 is a horizontal and vertical cross-sectional view showing the next state of FIG.
13 is a longitudinal sectional view showing the next state of FIG.
14 is a longitudinal sectional view showing another state of FIG.
15 is a longitudinal sectional view showing the next state of FIG.
16 is a plan view, a back view, a front view, a cross-sectional view, and a vertical cross-sectional view showing a part of FIG. 1 and another embodiment.
17 is a plan view, a back view, a cross-sectional view, and a vertical cross-sectional view of another embodiment of a part of FIG. 1;
18 is a plan view of the embodiment using FIG. 17 and a sectional view in the directions of III-III and VV.
[Explanation of symbols]
1 Frame base
1b Sample data location
1c Locking groove
1d Outer peripheral surface of frame-shaped substrate
1e Lower end surface
2 opening
3 Porous support bottom plate
3a outer peripheral surface
3b Locking dent
3c window
3d window
3e Bottom outer peripheral surface
3f porous
4 lid
4a Locking piece
4b Locking protrusion
4c Sample data description surface protection grid plate
4d lid tab
4e Lid window
4f Liquid hole in the lid
4g Locking hole of reversible lid locking protrusion of perforated dish
4h Sample data location on the lid
5 Dish-shaped perforated bottom plate
5a Inner peripheral surface
5b Locking protrusion
5c Inner circumference
5d top surface
5f Support
5g Plate-like perforated bottom tab
6 Embedding dishes
6a Inner peripheral surface
6b outer peripheral surface
6c Embedding dish tab
6d Embedding dish holder
6e Top surface of the embedding dish
7 Porous dish with lid (case for fixed support)
7a Perforated plate lid
7b reversible lid
7c Locking hole
7d biopsy storage compartment
7e Sample data location
7f Reference mark
7g Locking protrusion
7h perforated plate bottom
7i perforated dish bottom
7j Isobe
8 Biopsy (small pathological tissue) sample
8a Large pathological tissue sample
10 Embedded block samples
14 space
15 Molten paraffin (embedding agent)
16 Thin sword
18 Microtome adapter
19 Funnel
20 Lid
21 Depressurization
22 Fixing solution (fixing gelling agent)
23 Fixing support
24 Gelling fixing support
25 tweezers
26 Barcode
27 block sample data
28 labels

Claims (5)

An opening is formed on the upper side, and a frame-shaped base on which a porous support bottom plate with a window projecting in an area smaller than the outer peripheral surface of the bottom is formed on the lower side, a porous dish with a lid disposed in the frame-shaped base, In a cassette for pathological tissue sample comprising a plate-like porous bottom plate with a support that is detachably fitted to the outer peripheral surface of the porous support bottom plate with a window,
The outer peripheral surface of the window-supported porous support bottom plate is shaped to fit water tightly with the inner peripheral surface of the pathological tissue sample embedding dish, and the outer peripheral surface of the embedding dish and the outer periphery of the frame-shaped substrate of the pathological tissue sample cassette A cassette for a pathological tissue sample forming an embedding block of a biopsy small histopathological tissue sample characterized in that the surfaces almost coincide with each other, and an embedding dish thereof. A frame-shaped base having a porous support bottom plate formed with an opening on the upper side and projecting on the lower side with a smaller area than the outer peripheral surface of the bottom surface, and a dish-like shape that is detachably fitted to the outer peripheral surface of the porous support bottom plate In a cassette for pathological tissue sample comprising a porous bottom plate,
The outer peripheral surface of the porous support bottom plate is shaped to fit water tightly with the inner peripheral surface of the embedding dish of the pathological tissue sample, and the outer peripheral surface of the embedding dish and the outer peripheral surface of the frame-shaped substrate of the cassette for pathological tissue sample A cassette for a pathological tissue sample which forms an embedded block of the pathological tissue sample, characterized in that A sample data surface protection grid plate is inclined downward on one side of the lid of the opening of the frame-shaped substrate, and a block sample data label or bar code label is inclined downward on one side of the frame-shaped substrate. 3. The cassette for pathological tissue sample according to claim 2, wherein the label, the block sample data, and the barcode-printed printing surface are protected by being sandwiched between the frame-shaped substrate and the sample data-written portion. The pathological tissue sample according to claim 1, wherein the lid-equipped porous dish is sandwiched between a lower surface of the lid of the opening of the frame-shaped substrate and a support projecting from the upper surface of the dish-shaped porous bottom plate. Cassette and its embedding dish .  The dish-shaped porous bottom plate that is detachably fitted to the outer peripheral surface of the porous support bottom plate formed on the lower side of the frame-shaped substrate is removed from the outer peripheral surface of the porous support bottom plate, and the pathological tissue sample is placed on the embedding plate. The inner peripheral surface of the embedding dish is water-tightly fitted to the outer periphery, and molten paraffin is injected into a space surrounded by the inside of the frame-like base body and the upper side of the embedding dish, and a porous support bottom plate therein And a method for forming an embedding block, wherein a pathological tissue sample is embedded integrally.

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