JP2002303568A - Cassette for pathological tissue sample and method for forming embedded block using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cassette for a pathological tissue sample enabling to conduct a follow-up review of each condition later, while a pathological tissue sample is sampled by a clinician, is sent to a pathologist, an embedded block without burrs is formed there, and the result of a pathological examination is returned to the clinician. SOLUTION: The cassette for a pathological tissue sample includes a frame shaped base 1, a porous pan 7 with a cover arranged in the frame-shaped base 1, and a pan-shaped porous bottom plate 5 detachably engaged with an outer peripheral surface 3a of a porous supporting bottom plate 3, and is so formed that the outer peripheral surface 3a of the porous supporting bottom plate 3 is water-tightly engaged with an inner peripheral surface 6a of an embedding pan 6 of the pathological tissue samples 8 or 8a, and the outer peripheral surface 1d of the frame-shaped base 1 is made flush with the outer peripheral surface 6b of the embedding pan 6.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a method for preparing a pathological tissue sample taken from a patient's body into a thin section stained sample and returning it to the clinician in order to determine the diagnosis and treatment of the disease. The present invention relates to a pathological tissue sample cassette used in a system for managing a pathological tissue sample in between, and a method of molding an embedded block used therein.

Further, when forming an embedding block of a pathological tissue sample in a state before slicing the pathological tissue sample, the thickness at the time of slicing is made uniform by preventing burrs from being generated in the embedding block. In addition, in order to improve the accuracy of the pathological examination, burrs are generated around the conventional cassette due to its structure in the conventional embedding work process, and the burrs are removed with a knife or heat. Eliminating and streamlining the deburring work process, and using the conventional cassette and the deburring method using the embedding method, the sample data surface printed around the cassette is damaged and the sample data after the embedded block is manufactured This makes it difficult to read the document.

[0003] More specifically, a clinician collects a patient sample from each patient, transfers the collected sample to a pathological examination room or the like in a fixed manner, and thereafter cuts out the work from the reception / entry on the pathological examination side, and performs fixing / fixing. Chemical treatment of dehydration, degreasing, and penetration of the embedding agent, embedding work, slicing, section scooping, section extension and pasting work, sealing and storage management of sliced block samples, specimen staining and arrangement of completed specimens, During the period from the collation and confirmation of the slide specimen of each patient to the request form data and the input of diagnostic data at the time of pathological diagnosis, from the file and search work of the history data of each patient to the completion of the work of sending diagnostic data, etc. Barco suitable for the management system of each operation that enables the secure storage of each patient sample (with each patient sample, request information, a report to be described, and past data) and the management of its tracking Bonding the de or a two-dimensional data code label or the like, a related method of forming the embedded block using the same, and pathologic tissue samples cassette can be observed confirmation from the cassette outside the interior of the sample.

[0004] Still further, the present invention relates to a pathological tissue sample cassette used in a system for tracking and confirming each management of the pathological tissue of each patient after each stage and a method of molding an embedded block using the cassette. is there.

[0005]

2. Description of the Related Art Conventionally, in this type of work, a pathological tissue sample collected from a patient by a clinician is stored in a container on which a label or the like bearing the name of each patient or other data is attached. Then, along with the examination request form in which the attributes of each patient and the clinical diagnosis, findings, etc. are described, they are transferred by appropriate means such as mailing or messenger.

[0006] The specimen sample of each patient stored in the above-mentioned container transferred to the pathological examination side at a place remote from the clinician is converted into a patient specimen before a specimen preparation work for the pathologist to make a pathological diagnosis there. At the time of reception / entry, the request form for pathological diagnosis attached to the patient sample container at the time of reception / entry is checked against the name of each patient, the contents of the request, the status of the pathological tissue sample, etc., and the pathological tissue sample is Make sure it is.

[0007] Thereafter, at the time of reception and entry, the patient ID number and chart number of each patient at the time of collection of the pathological tissue sample by the clinician are also included in the pathological tissue sample collation random number at the time of collection / delivery of the patient sample or reception of the messenger sample. The pathological tissue sample or the biopsy pathological tissue sample of the patient, which is trimmed in another cassette (processing basket) in which the patient sample number is newly described by a serial specification number or a sample number, which is not related to After that, chemical treatment for specimen preparation is performed, all samples are picked with tweezers, etc., transferred to the bottom of the embedding dish, embedded with an embedding agent, attached to a stock (base), and embedded. Excess burrs around the block of the embedding agent formed after solidification of the agent are scraped off to prepare a block sample.

[0008] The block sample is sliced, stretched and attached to a slide glass, dried, and then unembedded (deparaffin).
A stained slide specimen is prepared by staining and enclosing the specimen, and a pathologist diagnoses the pathology by comparing the specimen sample of each patient with the request form data and the history data, and submits a pathological diagnosis report to the clinician. Is what you do.

In the above-described process, pathological tissue sample data of each patient (collected sample, request form data, and past history data)
During the period from the time of sampling by the clinician at the time of completion of the pathological diagnosis report to the time of completion of the pathological diagnosis report, inspections and checks to prevent loss, contamination, and replacement of each pathological tissue sample are performed by discontinuous human hands. The whereabouts of workers' responsibilities at the stage are also unclear after the fact.

[0010]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a clinician with a pathological tissue sample including a biopsy sample collected from each patient during the period from the time of collection to the time of completion of a pathological diagnosis report. Eliminates the risk of loss or contamination of sample data (collected sample, request form data, and past history data) of each patient, and the risk of human error in replacement, and in the event that such an error occurs, The purpose is to clarify where the trouble occurred and to clarify the responsibility.

Further, in the above-mentioned work process, human work is shortened or omitted in order to eliminate troubles that become unrecoverable and major problems due to simple human mistakes. This is to increase the number of work processes that can be monitored.

[0012] For this purpose, a macro image which allows the pathological tissue sample contained therein to be observed and confirmed from outside the perforated dish through the window of the fixed transfer container or the cassette window already applied for invention by the inventor, and their macro images. The patient identification data (patient attributes, patient ID, patient chart number, etc.) written and attached at the time of collection on the clinical side and the reception reference number on the pathology side collect the sample from the patient on the clinical side and Description of pathological diagnosis test request data including data and receipt / transport / reception / cut-out sorting of pathological test samples from the time of submission of pathological diagnosis request / chemical solution processing / embedding / sample preparation / history search / pathological diagnosis / data management / archiving -It should be managed and used in a unified manner in all work processes until the end of issuance of pathological reports.

For this purpose, in addition to the observation and confirmation of the macro image of the sample in the cassette, a means such as a sample data number, a bar code, a two-dimensional data code, etc. for securely linking and relating each patient data to the cassette sample is required. However, with the conventional embedded block manufacturing method, the data writing surface was damaged during the deburring operation of the cassette base and mechanical automatic reading could not be performed, so patient data from the clinical side was directly transferred to the pathological specimen manufacturing process side It could not be introduced. The purpose is to reliably protect the written surface of the block sample data that is also the patient sample data.

[0014]

According to the present invention, there is provided a cassette for a small pathological tissue sample, comprising a frame-shaped substrate having an opening on the upper side and a porous support bottom plate with a window on the lower side on the upper and lower sides, respectively. Patent No. 30495 filed by the inventor of the present invention
No. 37, Japanese Patent Application No. 7-300290 and Japanese Patent No. 3008
No. 078, a perforated plate and a fixed supporting case, a perforated plate-shaped lid or a perforated plate-shaped lid with a window fitted to the upper opening of the frame-shaped base, and a window portion of the lower perforated porous support bottom plate with a window. A support for the perforated dish protruding from an upper surface of a perforated dish-shaped bottom plate detachably fitted thereto, and a biopsy small pathological tissue sample cassette detachably fitted to the perforated support bottom plate with the lower window; In an instrument for forming an embedding block for a pathological tissue sample, comprising an embedding dish in which the outer peripheral surface of the frame-shaped substrate and the outer peripheral surface of the embedding dish almost coincide with each other, An inwardly projecting support that can be fitted and inserted into the inner periphery is formed on the dish-shaped porous bottom plate, and the inner peripheral surface of the dish-shaped porous bottom plate and the inner peripheral surface of the embedding dish are alternatively selected. Watertightly fitted to the outer peripheral surface of the window-supported porous support bottom plate protruding with a smaller area than the outer peripheral surface of the bottom surface of the frame-shaped substrate. Is shall.

The cassette for a large pathological tissue sample or fragment sample has a frame-shaped base having upper and lower sides formed with an opening on the upper side and a porous support bottom plate on the lower side, and fits into the upper opening of the frame-shaped base. The apparatus for forming a pathological tissue sample embedding block comprising a perforated plate-shaped lid to be fitted, and a dish-shaped porous bottom plate having no support that is detachably fitted to the lower window opening, wherein the dish-shaped porous bottom plate is provided. The inner peripheral surface of the embedding dish and the inner peripheral surface of the embedding dish are water-tightly fitted to the outer peripheral surface of the porous support bottom plate, which is alternatively protruded with a smaller area than the outer peripheral surface of the bottom surface of the frame-shaped base. The outer peripheral surface of the frame-shaped substrate of the pathological tissue sample cassette almost coincides with the outer peripheral surface of the embedding dish. At this time, for the dish-shaped porous bottom plate for the fragment sample that does not maintain a firm shape, a dish-shaped porous bottom plate having a smaller diameter than the porous portion of the dish-shaped porous bottom plate used for the other large-pathological tissue samples is used. Is desirable.

Further, a sample data-printed surface protection grid plate that protects the data-recorded surface such as a sample number, a barcode, and a two-dimensional data code, which is printed or affixed on the block sample data-printed portion of the cassette, from being damaged or peeled off. In addition to being molded integrally with the cassette lid, the conventional embedding molding method inevitably protrudes the block sample around the frame base of the cassette, and forms the block sample into the microtome adapter holder when the block sample is sliced. In order to prevent burrs from being obstructed in the embedding process in order to surely hold the product horizontally, a dish-shaped perforated bottom plate having no support body detachably fitted to the lower window opening is provided. In the instrument for forming a pathological tissue sample embedding block, the inner peripheral surface of the dish-shaped porous bottom plate and the inner peripheral surface of the embedding dish may be alternatively selected from the bottom of the frame-shaped substrate. The outer peripheral surface of the porous support bottom plate protruding with a smaller area than the outer peripheral surface is fitted in a watertight manner, and the outer peripheral surface of the frame-shaped base of the cassette for pathological tissue sample almost coincides with the outer peripheral surface of the embedding dish. It is. This is because, by changing the inner diameter of the perforations of the dish-shaped perforated bottom plate, the inner diameter of the perforations of the perforated support bottom plate, which is the bottom of the cassette, can be kept constant without changing according to the size of the sample. An embedding agent such as paraffin is poured into an embedding dish watertightly fitted to the outer peripheral surface of the porous support bottom plate projecting with an area smaller than the outer peripheral surface of the bottom surface of the base. Since no bubbles are generated and no burrs are generated, the deburring operation step is omitted, and damage to the block sample writing surface caused by this step is prevented.

[0017]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS As shown in FIGS. 1 to 3, an opening 2 is formed on the upper side of a frame-shaped base 1 and a porous support bottom plate 3 with a window 3c is formed on the lower side. Plate lid 4
Of the locking piece 4a at one end of the frame-shaped base 1
And a locking projection 4b in front of the surface protection grid plate 4c at the other end of the lid 4 is formed on the other side of the frame-shaped base 1 so that the sample data writing portion 1b of the frame-shaped base 1 is formed. And the perforated plate-shaped lid 4 is removably attached to the opening 2 of the frame-shaped base 1, and the outer peripheral surface 3a of the porous support bottom plate 3 with the window 3c is attached to the frame-shaped substrate. 1 and project downward from the lower end surface 1d.

Outer peripheral surface 3 of porous support bottom plate 3 with window 3c
The inner peripheral surface 5a of the dish-shaped porous bottom plate 5 is fitted watertightly and detachably to a. At this time, a locking recess 3b is formed on the outer peripheral surface 3a, and a locking protrusion 5b formed on the inner peripheral surface 5a is removably fitted to the locking recess 3b.
It is desirable that the lower end face 1d and the upper end face 5d of the dish-shaped porous bottom plate 5, and the outer peripheral face 3e of the lower end of the porous support bottom plate 3 and the inner peripheral face 5c of the dish-shaped porous bottom plate 5 are respectively closely adhered.

Further, instead of fitting the dish-shaped porous bottom plate 5 to the outer peripheral surface 3a of the porous support bottom plate 3 having the window 3c, an inner peripheral surface 6a of an embedding dish 6 used for embedding a biopsy sample. Alternatively, the inner peripheral surface 6a of the embedding dish 6 is detachably and watertightly fitted to the outer peripheral surface 3a, and the outer peripheral surface 1d and the lower end surface 1e of the frame-shaped base are connected to the outer peripheral surface 3a. Outer peripheral surface 6 of embedding dish 6
b and the upper end face 6e of the embedding dish almost coincide with each other.

In the figure, reference numeral 7 denotes a perforated dish with a lid used for storing a relatively small-sized biopsy sample (Japanese Patent No. 3049537 filed by the inventor and Japanese Patent Application No. Hei 7-300).
290 and Japanese Patent No. 3008078). 7a is its lid. The surrounding flange 7j is sandwiched and held between the lower surface of the lid 4 and a support 5f projecting from the upper surface of the dish-shaped porous bottom plate 5.

The biopsy sample cassette of the present invention has the above-described structure, and is used by a clinician to store the biopsy sample cassette in a predetermined biopsy storage section 7d of the perforated dish with a lid, and to use a suitable transport means to transport the pathologist to the pathologist. When making the embedding block 10 using the biopsy sample 8 in the perforated dish 7 with a lid transported to
As shown in the figure, the biopsy sample 8 submitted by the clinician is placed in the frame shape by fitting the dish-shaped porous bottom plate 5 on which the support 5f protrudes into the porous support bottom plate 3 with the window 3c. On the support 5f, which also protrudes inside the bottom of the base,
The perforated dish 7 with the lid together with the biopsy sample 8 is picked up with tweezers 9 and transferred into the frame-shaped base 1, and as shown in FIG. The plate 7 and the lid 4 are sequentially assembled. Thereafter, the pathological tissue sample together with the cassette is subjected to a chemical solution treatment process such as dehydration, degreasing, penetration, and penetration.

In the case of a large pathological tissue sample that does not fit in the perforated dish 7, a dish-shaped perforated bottom plate 5 not formed with the support 5f may be used as shown in FIG. At this time, the support 5f is not protruded in the frame-shaped base, and the maximum volume in the frame-shaped base can be widely used, and the trimmed sample obtained by cutting out the large pathological tissue sample is stored in the frame-shaped base. can do.

As shown in FIG. 4, it is also convenient to use a cassette bottom in which the window 3c is not formed in the porous support bottom plate 3 of the frame-shaped substrate. That is, the surgical extirpated material 8a is not always limited to a tissue having a fixed shape.
There is also an extirpated material (fragment sample) that scrapes the tissue of the affected part, which has a high risk of flowing out from the large through-hole of the cassette like the biopsy sample 8, and that a larger amount of tissue pieces than the biopsy sample 8 is used. You may have to deal with it. For such a case, as shown in FIG.
The bottom of the cassette (frame-like substrate 1) having a large through-hole in the porous support bottom plate 3 without c is formed, and the porous support bottom plate of the frame-like substrate 1 is adapted to the type and properties of the tissue sample to be treated with the chemical solution. The diameter of the perforated pores of the dish-shaped porous bottom plate 5 to be fitted to 3 can be selected and used. At this time, a similar result can be expected even in the case where a perforated plate as shown in the right diagram of FIG. 16 is fitted to the inner bottom surface of the cassette bottom (frame-like base 1).

When embedding the sample in which the embedding agent has penetrated after the chemical solution treatment process is completed, as shown in FIGS. The biopsy sample is picked from the dish or the fixed supporting case with tweezers 25 and placed on the inner bottom surface of the embedding dish 6. In the case of other large samples, as shown in FIG. 6, the sample is picked from the frame-shaped substrate 1 with tweezers 25 and the sliced inspection surface is directed downward on the bottom surface of the embedding dish 6 in the same manner as the biopsy sample. Place it flush. Thereafter, molten paraffin 15 is poured into spaces 14 formed above and below the porous support bottom plate 3 inside the frame-shaped base 1 and the embedding dish 6 in the state shown in FIG. 6d is held with the thumb and forefinger together with the embedding dish 6, not shown, but is transferred onto a cold plate of an embedding device and cooled, and the biopsy sample 8 is embedded together with the porous support bottom plate 3 to form an integrated solid state. Then, the tab 6c of the embedding dish is lowered with a finger to remove the embedding dish 6, and an embedding block sample 10 for slicing is formed.

At this time, since the outer peripheral surface 3a of the porous support bottom plate 3 constituting the bottom of the frame-shaped base 1 and the inner peripheral surface 6a of the embedding dish 6 are fitted in a watertight manner, When the paraffin 15 is filled, it passes through the gap between the fitted portions,
Furthermore, the lower end surface 1d of the frame-shaped base 1 and the upper end surface 6e of the embedding dish 6
Does not flow into the external space 13 through the gap between the outer peripheral surface 1 d of the frame-shaped base 1 and the outer peripheral surface 6 of the embedding dish 6.
Since the b is fitted flush, the molten paraffin (embedding agent) 15 attached to the periphery of the frame-shaped substrate 1 hardly stays at the boundary between the frame-shaped substrate 1 and the embedding plate 6 and the Runs down below 6. Therefore, when the paraffin 15 in those gaps is cooled and the embedding operation is completed and the embedding dish 6 is removed from the embedding block sample 10, the embedding block sample 1 is removed.
0b where the sample data is written on the frame-shaped substrate 1 which is also the base of 0
No burr of solidified paraffin (embedding agent) in the periphery containing.

Further, in the conventional cassette, the size of the inner diameter of the perforations 3f must be changed depending on the size of the pathological tissue samples 8 to 8a. However, as shown in FIG. Frame-shaped substrate 1 of porous support bottom plate 3
Is perforated 5h of the dish-shaped perforated bottom plate 5 with or without the window 3c.
Can be fixed at a fixed inner diameter because of the role. Therefore, it is not necessary to make the inner diameter of the perforations 3f too small, and the molten paraffin (embedding agent) 15 at the time of embedding is placed on the bottom of the pathological tissue samples 8 to 8a from the space 14 of the frame-shaped base (cassette bottom) 1. Air bubbles that are likely to be generated when pouring into the embedded embedding dish 6 can be suppressed. The both sides of the solid embedding block 10 thus formed are sandwiched between the holders 18a and 18b of the microtome adapter 18 shown in FIG. 9 and fixed thereto, sliced with the slicing knife 16, and slide glass. To form a preparation.

At this time, since the embedding block 10 formed by using the embedding block forming device of the present invention does not generate burrs of the embedding agent around the embedding block 10, the embedding block 10 is connected to the microtome adapter 18. Holder 18a, 1
8b, the surface can be accurately contacted and stably supported when pressed and held on the pressure-bonded surface of 8b. And the pathology can be easily inspected.

Further, since the burrs are not attached to the periphery of the frame base (cassette bottom) 1 which is also the base of the embedded block sample, it is not necessary to remove the burrs, and the burrs are removed from the frame base (cassette). The surface of the portion where the block sample data written on the periphery of the frame-shaped base (cassette bottom) 1 is not damaged when it is removed from the bottom 1.

Therefore, a perforated dish, a fixed support case, a fixed fixing support, a fixed transfer container, a biopsy sample storage device, etc., a pathological tissue sample cassette and its embedding plate, etc. When used together, the pathological tissue samples 8 to 8a collected or excised from the clinical site for pathological definitive diagnosis and treatment of the patient and the pathological diagnosis request information of the patient include the patient's ID number, attribute, If patient data such as a medical chart number or a patient embossed magnetic card is attached, the completion of pathological diagnosis report from the time of pathological examination reception cutout, the accuracy management and data management, and the automation and rationalization of search. It has become possible to utilize the three information of the patient data and the sample reference number which are image-coded and bar-coded or two-dimensional data-coded.

Although the present invention has been described with respect to the above-described embodiment, the present invention is not limited to the embodiment, and specific technical means for implementing the embodiment more effectively may be added, or It is possible to partially change the embodiment.

For example, if the shape shown in FIGS. 10 and 16 is adopted, the lid 4 of the cassette, the dish-shaped perforated bottom plate 5 and the embedding dish 6 can be made of metal stainless steel instead of synthetic resin, and can be recycled. Durability is also increased.

A biopsy sample is placed on the bottom surface 7i of the perforated dish 7 as shown in FIGS. 13 to 15, and the fixing support 23 (Japanese Patent No. 3049537 and Japanese Patent Application No. 11-76843) can be fixedly fixed and held on the outside and inside of the bottom surface 7i of the bottom 7h of the perforated dish 7. That is, a plurality of or fragile and fragile samples can be fixed and held in a single storage space 7d of the cassette or the perforated dish 7 without any partition, and the subsequent sample preparation work can be advanced as if it were one sample.

At this time, as shown in FIG. 11 and FIG. 12, when used together with a biopsy sample storage device for which the inventor has already applied for invention, a biopsy sample can be transferred from a patient to an endoscope or the like on the clinical side. The biopsy sample is not directly touched by the instrument except when the biopsy sample is sliced with the forceps and when the biopsy sample is sliced with a slicing knife. Eliminates sample crushing

Further, as shown in FIGS. 13 to 15, a frame-shaped base 1 provided with the fixing support 23 and the porous support bottom plate 3 provided with the window 3c, and a dish-shaped porous bottom plate 5 provided with the support 5f are provided on the perforated plate 7 as shown in FIGS. When used in combination, the biopsy sample is securely stored in the outer space of the bottom surface 7i of the bottom 7h of the perforated dish 7 and in the space in the cassette surrounded by the dish-shaped perforated bottom plate 5, and securely on the outer bottom surface of the perforated dish 7. Can be held. Thereafter, in an embedding operation after a chemical solution operation such as dehydration, degreasing, penetration, and penetration for preparing a specimen, the entire cassette is turned upside down as shown in FIG. 14 to remove the dish-shaped porous bottom plate 5 from the frame-shaped substrate 1. Instead, the above-mentioned embedding dish 6 is fitted and falls down again to bring it to the state shown in FIG.
And push down the perforated dish 7 in which the biopsy sample 8 is fixed and held on the bottom face 7i by the gel fixation support 24 toward the bottom face of the embedding dish 6 with tweezers 25, When the sample is adhered and held on the bottom surface of the embedding dish 6 due to the cooling and solidification of the embedding agent (paraffin) 15 of the sample on the bottom face, the perforated dish 7 is immediately pulled up again with tweezers 25 and gelled. Biopsy sample 8 integrated with fixing support
Only the remaining portions remain on the bottom surface in the embedding dish 6, the following is the same as in FIG. 8 described above, and thereafter the embedding block 10 can be formed in the same procedure. That is, in this embedding operation, the tweezers 25 can be embedded without directly touching the sample, so that the biopsy sample is not lost, mixed, or misplaced at the time of embedding.

As shown in FIG. 17 and FIG. 18, the lid 7a of the perforated dish or the fixed support case for which the inventor has already applied for the invention can be used even if it is separated. This lid part
As shown in FIG. 17, the REVERSIBLE LID 7b and the perforated dish bottom 7h are provided with a locking projection 7g on the lid and a locking hole 7 on the perforated dish bottom 7h.
However, when the biopsy sample is stored in a perforated dish and its flange 7j is sandwiched between the support 5f and the lid 4 of the frame-shaped substrate (cassette bottom) 1, However, the lid portion REVERSIBLE LID 7b and the locking portion of the perforated dish bottom 7h are not required. Therefore, REVERSIBLE L
The ID 7b is turned upside down, and as shown in FIG. 18, the inventor has already applied the invention to the perforated plate or the lid 4 of the frame-shaped base with a window, which is also a holding device for a fixed supporting case. By forming the locking hole 4g of the locking projection of the REVERSIBLELID 7b of the perforated dish 7 having the same diameter as the stopping hole 7c and the same distance between the holes, the raw material in the perforated dish sandwiched in the cassette is formed. The test sample 8 can be processed without moving and losing from the predetermined position of the perforated dish 7 during the chemical processing for preparing the sample, and the biopsy sample 8 can be observed and confirmed. When the lid 4 of the cassette at the time of embedding is opened, the The REVERSIBLE LID 7b, which is the lid part of the perforated dish, can be opened together with the lid 4, and the biopsy sample 8 of the perforated dish 7 in the cassette can be taken out without opening two lids each time.

[0036]

As described above, according to the present invention, the outer peripheral surface of the porous supporting bottom plate constituting the bottom of the frame-shaped substrate and the inner peripheral surface of the embedding dish are fitted in a watertight manner, and the outer peripheral surface of the frame-shaped substrate and the embedding dish are fitted. When the embedding of the pathological tissue sample and the porous support bottom plate by pouring molten paraffin into the space surrounded by the embedding dish and the frame-shaped substrate, Paraffin does not flow out to the outside through the watertightly fitted portion and does not stay at the boundary between the frame-shaped substrate and the embedding dish.

Therefore, even if the embedding agent (paraffin) is cooled and solidified, no burrs are formed on the molded embedding block, so that the embedding block is stably supported by the microtome holder. When slicing with a slicing sword, it can increase the uniformity of its thickness and its accuracy, improve the quality of pathological examinations, and is the base for embedded blocks because there is no deburring work. Does not damage the print surface of the frame-shaped substrate (bottom of the cassette) on which sample data is written.

Further, according to the present invention, the sample data-containing surface protection grid plate is formed on one side of the lid of the opening of the frame-shaped substrate so as to be inclined downward. Protects the upper part of the sample data entry area formed by inclining downward so that the bar code, two-dimensional data code, or sample data number, etc. written or affixed to the sample data entry area may be lost during various operations. There is no risk of peeling off from the substrate or disappearing due to various processing solutions.

Further, a biopsy sample inside a perforated dish housed in a cassette through a transparent perforated dish, which has already been filed by the inventor, through a perforated support bottom plate with a window or a perforated plate-like lid with a window. Can be surely observed and confirmed, and can be captured as a macro image. When this image and the image of the pathological examination diagnosis request form data from the clinic and the barcode, the two-dimensional data code, or the sample reference number are used for patient confirmation and sample tracking management, reliable accuracy control and mechanization with reduced labor are required. It will help to advance automation. Therefore, if a human error such as replacement of a biopsy sample occurs, the responsibility can be clarified by tracking even after the fact.

[Brief description of the drawings]

FIG. 1 is a longitudinal sectional view showing an embodiment of a biopsy sample cassette of the present invention.

FIG. 2 is a longitudinal sectional view in which FIG.

FIG. 3 is a perspective view of a part of FIG. 1;

FIG. 4 is a perspective view of a portion of FIG. 1 and a portion of another embodiment.

FIG. 5 is a plan view of an embodiment using a part of FIG. 1;

FIG. 6 is a perspective view showing another embodiment different from the embodiment of FIG. 5 described above, in which a part of FIGS. 3 and 4 is combined.

FIG. 7 is a plan view showing a state following the state shown in FIG. 5;

FIG. 8 is a longitudinal sectional view showing a state next to the state of FIG. 7;

FIG. 9 is a longitudinal sectional view and a plan view showing another state.

FIG. 10 is a plan view, a front view, a side view, a rear view, and a longitudinal sectional view showing another embodiment.

FIG. 11 is a horizontal and vertical sectional view showing another state.

FIG. 12 is a horizontal and vertical sectional view showing a state next to FIG. 11;

FIG. 13 is a longitudinal sectional view showing a state next to FIG. 12;

FIG. 14 is a longitudinal sectional view showing another state of FIG.

FIG. 15 is a longitudinal sectional view showing a state next to FIG. 14;

FIG. 16 is a plan view, a rear view, a front view, a cross-sectional view, and a vertical cross-sectional view showing a part of FIG. 1 and another embodiment.

FIG. 17 is a plan view, a rear view, a cross-sectional view, and a vertical cross-sectional view of another embodiment of a part of FIG. 1;

18 is a plan view of the embodiment using FIG. 17 and its III.
It is sectional drawing of the direction of -III and VV.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Frame-shaped base 1b Sample data description place 1c Locking groove 1d Outer peripheral surface of frame-shaped base 1e Lower end surface 2 Opening 3 Porous support bottom plate 3a Outer peripheral surface 3b Locking dent 3c Window 3d Window frame 3e Lower outer peripheral surface 3f Porous 4 Cover 4a Locking piece 4b Locking protruding piece 4c Surface protection grid plate with sample data 4d Lid tab 4e Lid window 4f Lid through hole 4g Ligable hole of reversible lid locking hole of perforated dish 4h Lid lid Location of sample data 5 Dish-shaped perforated bottom plate 5a Inner peripheral surface 5b Locking protrusion 5c Inner perimeter 5d Upper end surface 5f Support 5g Tab on perforated bottom plate 6 Embedding dish 6a Inner peripheral surface 6b Outer peripheral surface 6c Embedding dish Tab 6d Holder for embedding dish 6e Upper end face of embedding dish 7 Perforated dish with lid (case for fixed support) 7a Cover for perforated dish 7b Reversible lid 7c Locking hole 7d Biopsy storage section 7e Sample data described Place 7f Reference mark 7g Locking projection 7h Perforated dish bottom 7i Perforated dish bottom 7j Flange 8 Biopsy (small pathological tissue) sample 8a Large pathological tissue sample 10 Embedded block sample 14 Space 15 Melted paraffin (embedding agent) 16 Slicing blade 18 Microtome adapter 19 Funnel 20 Lid 21 Decompression housing 22 Fixing solution (fixing gelling agent) 23 Fixing support 24 Gelling fixing support 25 Tweezers 26 Barcode 27 Block sample data 28 Label

Claims (5)

[Claims] 1. A frame-shaped base having an opening formed on the upper side, and a porous support bottom plate with a window formed on the lower side thereof with a projecting area smaller than the outer peripheral surface of the bottom, and a lid disposed in the frame-shaped base. A pathological tissue sample cassette comprising a perforated dish (fixed support case) and a dish-shaped perforated bottom plate with a support removably fitted to the outer peripheral surface of the perforated support bottom plate with a window. The outer peripheral surface of the bottom plate is formed into a shape that fits tightly with the inner peripheral surface of the pathological tissue sample embedding dish, and the outer peripheral surface of the embedding dish almost coincides with the outer peripheral surface of the frame-shaped substrate of the pathological tissue sample cassette. A cassette for a pathological tissue sample forming an embedding block for a biopsy small pathological tissue sample, and an embedding dish thereof. 2. A frame-shaped substrate having an opening formed on the upper side, and a porous supporting bottom plate formed on the lower side and projecting with a smaller area than the outer peripheral surface of the bottom surface, and detachably attached to the outer peripheral surface of the porous supporting bottom plate. In a cassette for a pathological tissue sample comprising a fitted dish-shaped porous bottom plate, an outer peripheral surface of the porous support bottom plate is formed into a shape that fits tightly with an inner peripheral surface of an embedding dish for a pathological tissue sample, and the embedding dish is formed. A pathological tissue sample cassette forming an embedding block for a pathological tissue sample, wherein the outer peripheral surface of the pathological tissue sample cassette and the outer peripheral surface of the frame-shaped substrate of the pathological tissue sample cassette substantially coincide with each other; 3. A sample data surface protection grid plate is formed on one side of the lid of the opening of the frame-shaped base body so as to be inclined downward, and a block sample data label or a bar code label is formed on one side of the frame-shaped base body. 3. The label or the block sample data or a barcode printing surface of the label or the block is protected by being sandwiched between the frame-shaped base and the sample data-placed portion formed so as to be inclined. Cassette for pathological tissue samples. 4. A method of sandwiching a perforated dish with a lid (fixing support case) between a lower surface of a lid at an opening of the frame-shaped base and a support projecting from an upper surface of a perforated dish-shaped bottom plate. The cassette for a pathological tissue sample according to claim 1, wherein: 5. A dish-shaped porous bottom plate detachably fitted to an outer peripheral surface of a porous support bottom plate formed below a frame-shaped substrate is removed from the outer peripheral surface of the porous support bottom plate, and a pathological tissue is placed on an embedding dish. The sample is placed, and the inner peripheral surface of the embedding dish is watertightly fitted to the outer periphery thereof,
An embedding block characterized by injecting molten paraffin into a space surrounded by the inside of the frame-shaped substrate in this state and the upper side of the embedding dish, and integrally embedding a porous support bottom plate and a pathological tissue sample therein. Molding method.