JP2008026097A - Embedding frame-shaped substrate basket and embedding method using it - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a chemical treatment basket capable of preventing the size or the shape of a sample from being influenced in an embedding/slicing process by chemical treatment of a biosample such as immobilization, dehydration, defatting, permeation embedding agent penetration; a perfect burr-free embedding method of the sample; and a new sample preparing system including a perfect printing/installation method of a sample data storage media of an embedding block sample to be sliced. <P>SOLUTION: A sample preparing system is prepared, wherein an embedding dish-shaped apparatus function is used in common by the chemical treatment basket, and thereby an embedding block support base for slicing is allowed to function as an independent apparatus, to thereby release the embedding block support base for slicing from a chemical treatment basket function of the biosample. Hereby, shape selection between a shape of a cassette-shaped base which is thin or thick as before, a box shape and a ring shape can be performed freely without being influenced by the size or the shape of the biosample, and absolute necessity of dipping into a chemical liquid becomes unnecessary. Consequently, a perfect burr-free state can be realized when preparing the embedding block sample, and a working time for printing and installation of the sample data storage media can be taken sufficiently. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  This invention is a medical or research data for doctors and life scientists who decide on the diagnosis and treatment of diseases, or make biological specimens collected from patients or living organisms for the purpose of life research. The present invention relates to an embedding frame-like base rod used in a pathological tissue sample management system and an embedding method using the same.

None of the conventional cassette systems of any manufacturer has developed significantly from the specimen processing container apparatus and embedding method first proposed by Miles (USA) and developed from ring or frame cassettes. The new signs are about the burr-free embedding dish proposed by the present inventor and its method (see Patent Documents 1 and 2), and others are imitations of Miles' sample preparation and embedding methods and instruments. Only.
In the conventional cassette system, the so-called cassette substrate (specimen processing container) serves as both the cassette chemical processing bowl and the block base for slicing, and is used for the chemical processing bowl used for chemical processing when embedding blocks are made. The cassette base is used as a base, but the embedding dish has been proposed as an instrument prepared in advance.

  Therefore, in order to prepare a slide specimen of a biological sample, data such as a sample number is written or printed on a so-called cassette substrate (specimen processing container), and the biological sample is stored in the container, and the entire cassette substrate is dehydrated and degreased.・ Implement chemical processing for penetration and embedding agent penetration. Therefore, the full-scale use of this Miles-type cassette system starts from the start of sample cutting and trimming, and when collecting or transferring patient samples, when using other containers or when using the Miles-type cassette system, formalin, etc. The so-called cassettes contaminated with (1) are washed and wiped again before using the data, or the cassette containers are discarded and replaced with new cassettes.

  The biological sample stored in the cassette substrate is subjected to chemical treatment such as dehydration, degreasing, penetration, penetration of embedding agent, and transferred to the bottom of the embedding dish. It is installed at a predetermined position from the opening of the embedding dish, and the biological specimen is integrally embedded as a block sample using the cassette base as a block support base for slicing.

  Thereafter, the block sample is sliced by fixing the base to a cassette adapter for slicing microtome, and subjected to microscopic judgment and diagnosis after work steps such as data transfer, extension, dry pasting, staining, and encapsulation.

Japanese Patent Laid-Open No. 2002-303568 Japanese Patent Laid-Open No. 2004-125631

  Since the conventional technique is as described above, the cassette base is limited by the size, amount and shape of the biological sample to be accommodated, which also affects the role, size and shape of the container. For this purpose, it is reasonable to unify the size of the block sample and make it uniform, but the upper limit of the thickness of the biological sample suitable for chemical treatment such as dehydration, degreasing, penetration, embedding agent penetration, etc. is 5 mm. The size of the standard type of slide glass is approximately 26.5 mm x 76.5 mm, and the size of the conventional plastic cassette base is also in terms of material strength. Because the limited body size is 31 × 26 × 5 mm, the outer method is 40 × 28 × 6 mm, and the embedding agent in which the biological sample is embedded has a thickness of 5 to 6 mm, the final block sample The size of is 40 × 28 × 11-12 mm or more. For storage management of block samples, which are important life / gene information samples after slicing, it is necessary to reduce the storage space by reducing the thickness of the block base.

  In addition, the introduction of data storage media is indispensable for collating block samples with their organisms such as patient information such as medical history, associating them with data including images, automating specimen preparation, and building storage management systems. Is.

  However, since the cassette base of the conventional cassette system is used from the start of the chemical treatment of the biological sample for the convenience of serving as a treatment tank, the sample data of the biological sample is used at this time. The sample number and the like must be printed and printed on the data recording section, and before that, reliable data cannot be written. However, it is time-consuming to write, print, or print sample data such as sample numbers and barcodes in the data entry section at the time of cutting, and barcodes unless you are in a large pathological laboratory or laboratory. It is impossible to introduce a printer such as a two-dimensional data code.

  Furthermore, since the cassette substrate passes through the chemical treatment tank together with the biological sample, the sample data printed on the periphery and the data recording section are also affected by being immersed in the chemical solution. In particular, the current IC tag must be molded to be embedded in plastic or the like in advance. If it is immersed in a fixative such as formalin or an embedding agent such as water, alcohol, xylene, chloroform, acid, paraffin, etc. There is a high possibility of being destroyed.

  Furthermore, in the conventional cassette printer, depending on the accuracy of the ink and the printing method, the printed characters of the cassette base immersed in the embedding agent become unclear and difficult to read, or a data storage medium such as a barcode. It is difficult to read with other readers.

In view of the above circumstances, an object of the present invention is to reduce the storage space by reducing the thickness of a block support base.
Another object of the present invention is to record data on the block support base with a margin and to prevent the recording from becoming unclear.
Still another object is to provide three functions of an embedding frame-shaped base body that doubles as a chemical treatment container (frame-shaped base body) and an embedding dish-like instrument, a porous gutter lid, and an embedding dish-like instrument. Although it consists of one instrument part, by combining the shape function of the support base for thin-cut blocks with the shape function of the perforated lid part of the embedded frame-shaped base body に し, the functional shape is made into one function one instrument part, It is to reduce the cost of the equipment and improve workability.

  The present invention proposes a dual role as a so-called embedding frame-shaped substrate basket as a chemical treatment basket (frame-shaped substrate basket) and an embedding dish-like instrument used during embedding work. It has been proposed that a block support base for slicing used at the time of work is prepared in advance.

  The present invention also proposes a dual role as a so-called embedding frame-shaped substrate basket as a chemical treatment basket (frame-shaped substrate basket) and an embedding dish-like instrument used during embedding work. It has also been proposed that the block support base for slicing used at the time of burying work has a shape that can also serve as a lid for the upper opening of the embedded frame-shaped substrate ridge.

  In other words, the sample processing container (cassette base) previously announced by Miles for the first time serves as both a chemical processing bowl and a block support base for slicing. There is no specimen processing container (embedded frame-shaped base plate) as a new device that has a separate block support base for slicing, serving as both a chemical processing tube and an embedding dish-shaped device. .

  With this system, the conventional biological specimen preparation process and data sample storage management work have changed significantly in function, making data sample management search ubiquitous, automating work processes, and saving data sample storage management. Is going on.

  The present invention does not use a sample processing container (cassette substrate) in a conventional cassette system as a chemical processing vessel for storing a biological sample and processing a chemical solution. Since the size of the slicing block base is not affected by the size and amount of biological samples to be processed with chemicals such as dehydration, degreasing, penetration, and penetration of embedding agents, the size of the base is This is because the size and shape can be freely selected, for example, plate shape, block shape, conventional cassette shape or box shape, or ring or frame cassette shape, depending on the purpose and necessity.

  In addition, when the block support base for slicing is used as an instrument functionally independent from the role of the chemical treatment vessel, the block support base for slicing is fixed in the working process.・ Because it is not immersed in an organic solvent such as acid, alcohol, xylene, chloroform, etc., it is not affected or affected by these chemical solutions, and the time required for chemical treatment of biological samples is stored on the block support base for slicing. It can be used for printing and printing data storage media.

  This is because the sample processing container (cassette substrate) is required in the conventional cassette system at the start of cutting, and the cutting assistant manually writes the sample number, its branch number, etc. in the data recording section of the cassette substrate, or the cutting chamber. It is necessary to install an expensive bar code printing machine, etc., or to print random numbers in advance on the data recording part of the cassette base with bar codes etc. and associate these random bar codes with each sample data at a later date. However, in the present invention, the sample processing container required at the start of cutting is an embedded frame-shaped base rod that is a chemical processing rod and does not require a block support base for slicing, so the sample number to the block base・ Because there is enough time for printing and printing data such as branch numbers and data storage media such as barcodes and 2D data codes, these printing machines can be installed in different rooms or In summary it also becomes possible to have.

  When printing and printing data storage media and characters such as sample numbers, barcodes and two-dimensional data codes on the data recording part of the block support base for slicing at this time For slicing with a laser beam without using a conventional printer The data recording part of the block support base may be engraved with data storage media such as target characters and barcodes. At this time, in order to clearly represent a data storage medium such as a target character or barcode for the data recording part of the block support base for slicing, the block support base surface coating deposition member to be engraved and cut The block support base engraving that emerges as a letter or symbol in the engraved part and the double-layer synthetic resin of different colors of the adhesion developing member or the overlapping covering structure of the coating or plating process or the vapor deposition agent such as aluminum thin is the above laser beam It seems to be good to use the printing method that is glued and colored by engraving.

  At this time, if the sample number or barcode is printed on a small label or a piece of paper with an inexpensive printer, or if the sample number is handwritten and placed on the biological sample in the chemical treatment container, the biological sample is wrapped. The sample data print label is transferred onto the perforated bottom of a predetermined thin block support base prepared separately at the time of filling operation, and the data of the block support base and small label paper pieces (sample data print label) are collated. Thus, if the biological sample is embedded together with the embedding agent together with the block support base for slicing, it is inevitable that an error in inputting the biological sample and sample data such as its attributes will never occur. That's it.

  Another object of the present invention is that the functional part of the block support base for slicing corresponds to the shape and size of the opening of the embedding frame-shaped substrate tub which is a chemical processing tub and is an embedding dish-like instrument. If so, the embedding operation can be carried out using a conventional cassette-shaped, box-shaped, ring-shaped, frame-cassette-shaped, or thin plate-shaped / block-shaped block support base.

  Further, as described in the paragraph number “0014”, when the block support base for slicing is formed into a shape that can also be used as the lid portion of the upper opening of the embedded frame-shaped base plate, the embedded frame Organisms stored in the embedded frame-shaped substrate cage that has been treated with a chemical solution by removing the lid of the lower opening of the cylindrical substrate cage and injecting a small amount of molten paraffin into the bottom of the dish-like instrument on the hot plate Transfer the sample and lightly press the thin sliced surface against the bottom of the dish-shaped instrument. Molten paraffin which is placed on the bottom of the dish-shaped instrument in a shape and is completely immersed in the porous bottom of the block support base for slicing from the upper opening where the block support base for slicing is fitted An embedding dish shape filled with paraffin together with a dish-shaped instrument after injecting to the water level The ingredients were transferred to the cold plate, the molten paraffin can be integrally embedded and the block support base for slicing a biological sample solidified by cooling.

In the so-called cassette system based on the conventional specimen processing container apparatus and embedding method proposed by Miles for the first time, the cassette base serves as both a specimen sample chemical processing container and an embedding block support base for slicing, It was a system in which the embedding dish existed as an independent instrument container device. In contrast, since the present invention is configured as described above,
(1) The embedding block support base for slicing is a single independent functional instrument.
(2) On the contrary, the porous bottom embedding block support base for slicing also serves as a lid for a chemical solution processing basket for dehydration, degreasing, penetration, penetration of embedding agent, etc. Each part of the embedded frame-shaped base body with a block support base porous lid fulfills three functions: a chemical solution processing function, an embedding dish-like instrument function of the sample stored therein, and a block support base function for slicing It is united with.

  Therefore, in the case of the above (1), the embedding block support base for slicing is not involved at all during the chemical treatment of a biological sample. Therefore, it will serve as a necessary embedding block support base instrument after embedding after completion of chemical treatment such as dehydration, degreasing, penetration, embedding agent penetration, etc. The time until the completion of the chemical treatment after the fixation of the sample is the number of the sample data corresponding to the block support base for slicing, the barcode, the two-dimensional data code, the printing of data storage media such as magnetism and IC tags, The processing time such as writing can be multiplied.

  In addition, since only the function of the embedding block support base device is sufficient, the shape and thickness of the support base itself and the size of the liquid passage hole on the bottom surface can be freely selected according to the convenience of the work site. Since the block support base itself is not immersed in the chemical solution, this block sample is not completely burred, and any sample data storage medium or recording section is not contaminated or destroyed by the chemical solution or high temperature. It is.

  Further, in the case of (2), the slicing porous bottom-embedded embedded block support base also serves as a lid for a chemical treatment vessel such as dehydration, degreasing, penetration, penetration of embedding agent for biological samples. Thus, each part of the embedding block support base embedded frame-like base body with a porous lid has a chemical solution processing function, an embedding dish-like instrument function of a sample housed therein, and a block support base function for slicing Combined to fulfill three functions. Therefore, after the chemical treatment of the biological sample is completed, the lid portion attached to the lower opening of the embedded frame-shaped substrate 籠 is removed, and the biological sample to be embedded by chemical treatment is taken out of the lid, and a separately prepared dish shape After injecting a small amount of paraffin embedding agent into the instrument, transfer it to the bottom with the slicing inspection surface of the biological sample down, and lightly press on the cold plate to record the data such as the sample number on the biological sample. Is mounted on the porous bottom from the opening of the porous bottom block support base for slicing fitted on the upper side of the embedded frame-shaped substrate, and the embedded frame-shaped substrate from above the biological sample. After placing the paraffin embedding agent on top of it, transferring it onto the cold plate and completing the embedding work, block sample Remove the complete thin burr Since embedded block specimen is completed, it can be sliced.

  In addition, the block support base for slicing the embedded block of (1) is not originally used in a biological sample chemical treatment process, and the embedded block support base embedded with a porous lid of (2) above. The block support base for slicing used as a frame-shaped base rod is fitted as a lid portion to the embedded frame-shaped base having a completely embedding dish-like instrument function at the time of embedding work. The inner peripheral surface of the substrate and the block support base for slicing or the outer peripheral surface of the porous support bottom plate have a watertight structure, and the block support for slicing produced and molded from these embedding system steps There is no burr on the base.

  Furthermore, since a biological sample is not put into the structure in the block support base for slicing from the above, the shape and thickness of the support base itself and the size of the liquid passage hole on the bottom surface are Depending on the circumstances of the specimen preparation site, various shapes can be selected from the conventional shape to a new plate shape, contributing to space saving of specimen data storage, automation of specimen preparation and trouble prevention.

  Furthermore, since the thickness and size of the block support base for thin cutting can be made uniform at the same time as the unification of chemical treatment processes such as sample dehydration and embedding processes, It is easy to fix the block support base for slicing and to put in and out the block sample, and it is possible to embed the IC tag at the time of molding the block support base for slicing and write data later. .

  Further, not the printer that prints and prints with ink on the block support base for slicing, but the data recording part of the block support base for slicing itself is carved into the block support base and the adhesion-developing member and the surface coating vapor deposition covering it Since it has been molded into a two-layer structure of the member, the surface is engraved with a laser, the block support base is engraved, the adhesion developing member is exposed, and the data storage media such as the sample number and barcode are written. The printing data does not dissolve or disappear in organic solvents or paraffin embedding media like the ink printers in the past.

  Furthermore, the so-called embedding frame-shaped substrate い な い does not serve as a block support base for slicing, or if it also serves as a thin perforated lid portion, Since the data recording paper such as the sample number is put inside, it can be used from the time of patient sample collection on the clinical side, and the recording paper such as the patient number data is stored in the embedded frame-like base body cage. Since it can be stored together with the collected sample, it is certain that the responsibility will be clarified if it occurs while preventing mistakes in taking the sample. That is, the reasonable application range of the system of the present invention is greatly expanded from the time of patient sample collection to the time of completion of slicing as compared with the conventional cassette system.

  Furthermore, if the embedded frame-shaped substrate 籠 is manufactured as a reusable product such as a metal, it can be recycled including the lid portion of the embedded frame-shaped substrate 以外 except for the block support base for slicing. It can be used, resulting in a significant cost reduction.

The embedding frame-shaped base rod 7 having a central function according to the present invention is as shown in FIGS. One of the two functions of the embedded frame-like base body 7 is that the biological sample 3 that has been cut and trimmed is lost or mixed when the biological sample 3 is subjected to chemical treatment such as dehydration, degreasing, penetration, penetration of the embedding agent, etc. This is a function of the instrument and scissors to prevent the above and prevent the chemical treatment of the sample.
The embedding frame-like base body 7 includes an embedding frame-like base body 1 and a pair of upper and lower eyelid lid portions 2 and 2. The embedded frame substrate 1 is a cylinder having a rectangular shape in plan view, and the upper and lower sides thereof are openings 1A and 1A. On the upper end side and the lower end side of the outer periphery on the short side of the base body 1, lid latching portions 1 a and 1 a are projected.

The said lid part 2 is equipped with the rectangular frame part 2A contact | abutted to the end surface of the said embedding frame-shaped base | substrate 1, and the porous part (net | network part) 2B enclosed by this frame part 2A.
On the lower surface of the frame portion 2A, a lid cover locking portion 2a that locks to the lid latching portion 1a is projected. Two each of the locking portions 2a are provided on the short side, but the number, shape, arrangement position, and the like are appropriately selected as necessary. In the figure, 1b is a reference mark for indicating directionality, 4 is a sample number data sheet, and 5 is a sample number.
The main body 7 of the embedded frame-like substrate containing the biological sample 3 is subjected to chemical treatment of dehydration, degreasing, penetration, and penetration of the embedding agent together with the basket.

Other embedded frame substrates:
FIGS. 3 to 8 illustrate typical examples of the embedded frame-like base body 7. The same reference numerals as those in FIGS. 1 and 2 have the same names and functions.
FIG. 3 shows the most standard shape. In the embedded frame-like base rod 7 of the type shown in FIGS. 1 and 2, the upper and lower side lid lid portions 2 can be used in the same shape.

  In FIG. 4, the support part 13b of the block support base 11A for slicing is formed on the upper opening 1A side, and any type (for example, plate-like / block-like / conventional) can be used as long as the shape on the opening 1A side matches. It is also possible to freely select a block support base 11A for slicing in a cassette shape, box shape, ring or frame cassette shape and its size and shape.

  FIGS. 5 to 7 show a block support base for slicing in the shape of a so-called cassette base 11 forming a data recording part (the lid-inclined part 2c) which is currently manufactured and sold by many manufacturers. It is explanatory drawing of the embedding frame-shaped base | substrate cage | basket 7 for base 11A. The same reference numerals as those in FIGS. 1 to 4 have the same names and functions.

  5A is a longitudinal sectional view, FIG. 5B is a side view thereof, FIG. 6A is a plan view, FIG. 6B is a front view, and FIG. 7 is a slope view thereof. . In addition, 21a shows an embedding frame inner wall.

  FIG. 8 shows that the lid 2 on the side of the upper opening 1A of the embedded frame-like base body 7 is formed into the shape of a block support base 11A for thin cutting (also used as a substitute for a block support base for thin cutting). It is the longitudinal cross-sectional view made into the heel cover part)). FIG. 8A shows that the perforated support bottom plate 18 protrudes in an area narrower than the outer periphery of the bottom surface of the thin block support base 11A for thin cutting, and the outer periphery of the perforated support bottom plate 18 1 is formed into a structure that fits water tightly into the inner periphery of the upper opening 1A.

   In addition, 11B shows a block support base porous bottom plate, 11C shows a block support base opening part, and 11D shows a block support base porous bottom plate part, respectively.

  FIG. 8 (B) shows that the lid 2 on the upper opening 1A side of the embedded frame-shaped substrate 籠 7 is formed into the shape of a block support base 11A for slicing, and is also used as a lid (“block support base for slicing”). A support section 13b on which the thin-cut block support base 11A is placed is formed on the upper opening 1A side of the embedded frame-shaped substrate 1, and is formed in the opening section. A thin plate-like or box-like thin-cut block support base 11A is molded on the support portion 13b in a watertight manner.

   FIG. 8 (C) shows that the lid 2 on the upper opening 1A side of the embedded frame-shaped base body 7 is formed into the shape of a block support base 11A for thin slicing (("block support for thin slicing"). A support section 13b for mounting the outer periphery of the block support base 11A for slicing is formed on the upper opening side of the embedding frame-like base body 1, A porous support bottom plate 18 is protruded in an area narrower than the outer periphery of the bottom surface of the thin plate-like or block-like block support base 11A placed on the support portion 13b in the opening. The outer periphery of the support bottom plate 18 is molded into a structure that fits into the inner periphery of the lower side of the support portion 13b.

Embedding process:
9 to 13 are views showing an embedding block procedure using the embedding frame-shaped base rod 7. FIG. 9 is an operation diagram in which the embedded frame-shaped substrate 1 with the lid 2 used in the chemical treatment removed is moved onto a metal flat plate 33 serving as a bottom plate. The metal flat plate 33 is formed in a rectangular shape and has an area larger than that of the rectangular embedded frame-shaped substrate 1.

  FIGS. 10 and 11 show that the metal flat plate 33 is attached to the lower periphery of the lower opening of the embedded frame-shaped substrate 1 with the cooling and solidifying power of the embedding agent paraffin and the surface tension 41a. It is the figure which adheres to the lower opening part of the base | substrate 1, produces an embedding dish-like instrument, inject | pours a small amount of paraffin embedding agents 14, and picks and transfers the biological sample 3 with the tweezers 34 in it. .

  Furthermore, FIG. 12 shows that the lower sliced surface of the biological sample 3 is lightly pressed from above with the tweezers 34 against the bottom of the container which is the embedding dish-like instrument on the cold plate 50, and the cooling and solidifying force of the embedding agent is used. As shown in FIG. 13, the block support base 11A for thin slicing is fitted in a watertight manner to a predetermined portion of the embedding frame-like substrate 1 that is an outer frame part of the embedding dish-like instrument, as shown in FIG. Then, the embedding agent 14 is replenished and filled from above to embed the biological sample 3 and the slicing block support base 11A integrally. In addition, 30 is a data storage medium installation part such as an IC tag, 35 is a data storage part forming frame, 36 is an embedding center embedding agent inlet, 11e is a window, and 11f is a lattice.

  FIG. 14 shows an example of an IC tag that is stored and installed immediately before the embedding is completed at a predetermined position of the block support base 11A for slicing that is independent of the function of the chemical treatment vessel for the biological sample and is not immersed in the chemical solution. FIG. In FIG. 14, 37 is a stripe IC tag (FIG. 14A), 37a is a side surface of the stripe IC tag (FIG. 14A), 38 is a label IC tag (FIG. 14B), and 38a is Side surface of label type IC tag (FIG. 14B), 39 is a coin type IC tag (FIG. 14C), 39a is a side surface of coin type IC tag (FIG. 14C), and 40 is sesame grain IC Tags (FIG. 14 (D)) and 40a are side faces of the sesame grain IC tag (FIG. 14 (d)), respectively.

FIGS. 15 and 16 show the storage timing and installation location of the sample number data sheet 4 and the IC tag 37 to be stored and installed.
The IC tag 37 shown in FIG. 15 is sandwiched between tweezers 34 and stored in the media installation unit 30 as shown in FIG.

Further, FIG. 17 shows how to remove the block sample 51 for slicing in an embedding method using an embedding dish-like instrument comprising the embedding frame-like substrate 1 and a bottom plate (metal flat plate) 33.
As shown in FIG. 17, when the embedding frame-shaped instrument is sandwiched with both hands 42 with the embedding frame-shaped substrate 1 facing down and the embedding agent 14 is pushed up with the thumb 42a, the sliced block sample 51 is obtained. , Easily removed from the substrate 1.

FIG. 18 is a diagram in which the thin-cut block sample 51 is removed, and the sample data 4 and the IC tag 37 are also attached. It is obvious that the thin-cut block sample 51 completed at this time is a “burr-free block sample” disclosed in Patent Documents 1 and 2.
FIG. 19 is a diagram in which the thin block sample 51 is thinly fixed to an adapter 31 that is fixed to a thin microtome when a conventional thin block support base (11A) having a conventional thickness is used. It is. In FIG. 19, 31a is an adapter cassette fixing device, 31b is an adapter cassette fixing claw, 11H is a latching portion (claw engaging portion) of the adapter cassette fixing claw, and 32 is a microtome sword.

FIGS. 20 to 24 are views showing an embedding block procedure of an embodiment having a shape different from that of the embedding frame-like base body 7 and the thin-cut block support base 11A shown in FIGS. 9 to 13.
FIG. 21 is a work diagram in which the embedding frame-like base body 1 with the lid 2 used in the chemical treatment removed is moved onto the dish-like instrument 33a serving as the bottom plate.

  Further, FIGS. 22 and 23 show that the dish-shaped instrument 33a is embedded by the cooling and solidifying power and surface tension of the embedding agent paraffin adhering to the lower part of the lower opening of the embedding frame-shaped substrate 1 and the embedding frame. It is the figure which adheres to the lower opening part of the glass-like base | substrate 1, produces an embedding dish-like instrument, inject | pours a small amount of paraffin embedding agents 14, and transfers the biological sample 3 in it.

  FIG. 24 shows that the lower sliced surface of the biological sample 3 is lightly pressed on the cold plate 50 against the bottom of the container, which is the embedding dish-like instrument, with the tweezers 34, and flattened by the cooling solidification force of the embedding agent. As shown in FIG. 24A, the thin-cut block support base 11A is water-tightly fitted into a predetermined portion of the embedded frame-shaped substrate 1 which is an outer frame part of the embedded dish-like instrument. Then, the embedding agent is replenished and filled from above, and the biological sample 3 and the slicing block support base 11A are embedded integrally. FIG. 24B is a view in which the thin-cut block sample 51 is removed from the embedded frame-shaped substrate 1.

  25 to 30 show a block sample for slicing by being watertightly fitted to the upper opening 1A of the embedding frame-like base 1 of the embedding frame-like base rod 7 and embedding it integrally with the sample. 51 is an example of a block support base 11A for slicing similar to the shape of a conventional cassette base 11 that can be molded and manufactured, and can also be used as a conventional cassette system, but a data storage medium installation section such as an IC tag In addition, devices such as windows are added.

  That is, if the bottom portions of these block support bases are formed in accordance with the shape and size of fitting into the inner diameter of the upper opening of the embedded frame-shaped base 1, FIGS. 25 to 27 are box-shaped, and FIG. 30 to FIG. 30 show the shape of a conventional standard type, and FIGS. 31 and 32 can serve as a block support base 11A for thin slicing which is commonly called a ring or a frame cassette.

  At the specimen preparation site, depending on the preference or way of thinking of each site worker, the shape of the block support base 11A for slicing or the data description surface inclined by the conventional cassette system is formed in terms of functionality. There are a variety of sites that use the popular shape cassette base, box-type base, ring / frame cassette base, and root support and block support bases in the shape of rootstock. It becomes possible to meet the demand.

  In the figure, 11a is a cassette data recording section, 11b is a biopsy sample chamber storage section, 11c is a cassette base opening, 43 is a ring or frame cassette table, 43a is a ring or frame cassette back, and 43b is a ring lattice. , 43c indicate ring microtome fixing portions, respectively.

  FIG. 33 is a view of embedding the ring (frame cassette) -shaped slicing block support base 11A. Finally, the sample number data recording paper 4 is pasted on a predetermined place not interfering with the support base 11A with an embedding agent 14 so that it can be seen from the outside, and the specimen sample number 5 and the barcode 4a are collated and confirmed. Alternatively, the IC tag 37 is stored and installed in a predetermined location (IC tag or other data storage media installation unit 30).

34 to 49, the shape or functionality of the thin-cut block support base 11A, which can be fitted with the embedding frame-shaped substrate 1 according to the present invention to produce the thin-cut block sample 51, is used. It is explanatory drawing of a representative Example.
34 to FIG. 41 are diagrams for preventing the falling into the embedding dish-like instrument of the block support base 11A for slicing inside the upper opening of the embedding frame-shaped substrate 1, and for slicing. The rise of the embedding agent for molding the adapter cassette fixing claw latching portion (claw engaging portion) 11H which is the fixing portion of the thin-cut block support base 11A of the microtome adapter 31 cassette fixing device 31a, Without forming a so-called support portion 13b that serves to lower the block support base 11A by a certain width from the outer periphery of the bottom of the block support base 11A for thin slicing, it has that function in the shape of the block support base 11A for slicing accordingly. It is explanatory drawing of the embedding trough system of the embedding frame-shaped base | substrate 1 and the block support base 11A for thin slices.

  42 to 49 are illustrations for preventing slicing in the embedding dish-like instrument of the block support base 11A for slicing inside the upper opening of the embedding frame-shaped substrate 1, and for slicing. The rise of the embedding agent for molding the adapter cassette fixing claw latching portion (claw engaging portion) 11H which is the fixing portion of the thin-cut block support base 11A of the microtome adapter 31 cassette fixing device 31a, A so-called support portion 13b is formed which serves to lower the block support base 11A by a certain width from the outer periphery of the bottom of the block support base 11A for thin slicing, and the functional structure needs to be formed in the shape of the block support base 11A for slicing. It is explanatory drawing of the embedding rod system of the embedding frame-like base | substrate 1 and the thin block support base 11A which can make the structure of the thin block support base 11A simple and thin.

  FIG. 34A shows an embedded frame in which the upper and lower lid portions 2 of the embedded frame-like substrate basket 7 without the support portion 13b of the present invention are removed, and the biological sample 3 that has been stored therein and treated with a chemical solution. Data storage medium such as an IC tag in the form of a ring (frame cassette) which is one of the block support bases 11A for slicing in an embedding dish-like instrument formed by a base 1 and a base plate (metal flat plate) 33 It is the figure which is solidified and embedded integrally with the block support base 11A for thin cutting which shape | molded the installation part 30. FIG.

  FIG. 34 (B) shows the block sample 51. The embedding agent covers the entire surface of the bottom surface 43 fitted inside the upper opening of the embedding frame-like substrate of the ring (frame cassette), and directly from the outer periphery thereof. The block embedding agent 14 is standing up. The microtome for slicing the ring (frame cassette) is fixed by the block fixing portion 27 of the microtome.

This can be said to all of FIGS. 35 to 41, but the entire bottom portion of the portion where the block support base 11A for thin slicing is fitted in the upper opening of the embedded frame-like substrate 1. An embedding agent is put on and the block stands directly from the outer periphery.
As shown in FIGS. 19 and 52, the adapter cassette fixing claw 31b of the cassette fixing device 31a of the adapter 31 of the microtome for slicing is latched on the outer periphery of the bottom surface of the block support base 11A for slicing. This is because the claw engaging portion 11H that can be formed is molded, so that the block support base 11A for slicing can be fixed.
However, the block support base 11A for slicing in FIG. 35 is an old rootstock-like base, and is fixed by a block fixing portion of a microtome for slicing.

Further, as shown in FIG. 41, the block support base 11A for slicing is not used as a stored chemical solution processing basket for the biological sample 3. Therefore, the shape can be freely selected and thinned, and conversely, the frame-like substrate 1 of the embedded frame-like substrate 担 う 7 serving as the chemical solution treatment vessel of the biological sample 3 is formed in the shape of the lower opening thereof. If it is used together with a dish-shaped instrument 33a suitable for the above, a specimen having a thickness or length can be embedded vertically to prepare a specimen.
The thin-cut block support base 11A has the same outer shape as that shown in FIG. 37, but the material and structure for adhesive connection with the embedding agent are the same as those shown in FIG.

FIG. 36 shows a block support base 11A having a certain thickness, a window 11e at the bottom, a sample number data recording paper 4 attached to the upper part of the recess, and an IC tag or the like around it. A data storage medium installation unit 30 is vertically formed.
FIG. 37 is thinner than the thickness of the block support base 11A for slicing in FIG. 36 and has a window 11e at the bottom, but the thickness for vertically forming the data storage media setting part 30 such as an IC tag around it. The sample number data recording paper 4 and the IC tag 37 are affixed horizontally and stored in a recess at the bottom of the block support base 11A for slicing. In both FIG. 36 and FIG. 37, the data recording part 11a is formed on the embedding side of the biological sample 3.

  Further, in FIG. 38, there is no window at the bottom like the block support base 11A for slicing in FIG. 36 and FIG. 37, and the bottom consists of a porous bottom 11d. As shown in FIG. 38, the protrusion is short and narrow with the role of only the claw engaging portion 11H of the cassette fixing device 31a of the adapter 31 of the microtome for slicing. Therefore, the sample number data recording paper 4 and the data storage medium setting part 30 such as an IC tag are located in the upper part of the concave portion of the porous bottom part 11d.

39, 40, and 41, the bottom of the block support base 11A has no molded porous structure, and is made of a porous material, for example, a wooden / foamed plastic or a synthetic resin having a roughened outer peripheral surface. Molded with. 39 and 40, the bottom surface of the thin-cut block support base 11A protrudes, and the claw engaging portion 11H of the cassette fixing device 31a for the adapter 31 of the thin-cut microtome has a narrow shape on the outer peripheral portion.
The upper side of the bottom protruding portion of the block support base 11A for slicing in FIG. 40 is formed into a concave shape, and the sample number data recording paper 4 and the data storage media setting portion 30 such as an IC tag are in the concave portion. In FIG. 39, the recess of FIG. 40 is not molded.

  FIG. 42 (A) shows the biological sample 3 that has been subjected to chemical solution treatment by removing the upper and lower lid portions 2 of the embedded frame-shaped substrate cage 7 having the support portion 13b of the present invention. It is transferred into an embedding dish-like instrument formed of the embedded frame-shaped substrate 1 and a metal flat plate (bottom plate) 33, and the ring (frame cassette) -shaped one of the thin-cut block support bases 11A is formed. It is the figure which is solidified and embedded integrally with the block support base 11A for thin slices which shape | molded the data storage media installation parts 30, such as an IC tag.

  FIG. 42B shows the block sample 51, which is a support portion formed on the embedded frame-shaped substrate 1 from the entire surface of the bottom surface 43 fitted in the upper opening of the embedded frame-shaped substrate of the ring. The embedding agent is covered and solidified by the width of 13b, and the block embedding agent 14 rises from the position. The microtome for slicing the ring (frame cassette) is fixed by a block fixing portion 27 of the microtome.

  This can be said to be the same in all of FIGS. 43 to 49, but the thin block support base 11A is placed on the support portion 13b in the upper opening of the embedded frame-shaped substrate 1. In addition, the embedding agent is narrowly covered and solidified by the width of the support portion 13b on the entire bottom portion of the portion fitted inside the opening, and the block embedding agent 14 rises 1 from the position. . That is, when the outer peripheral portion of the bottom surface of the block support base for slicing is used as a claw engaging portion 11H that can be latched by the cassette fixing claw 31b of the adapter fixing device 31a of the adapter 31a of the slicing microtome, the biological sample 3 This is because the block embedding agent 14 which is embedded integrally with the block support base 11A for slicing is not interrupted by the fixing claw 11H of the cassette fixing device 31a of the adapter 31 to be broken and peeled off.

  Further, as shown in FIG. 49, since the block support base 11A for slicing is not used as a chemical treatment container for the biological sample 3, its shape can be freely selected and can be made thin. If the frame-shaped substrate 1 of the embedded frame-shaped substrate bag 7 serving as the chemical solution processing rod of the biological sample 3 is overlapped and the lower opening thereof is used in combination with the dish-shaped instrument 33a or the metal flat plate 33, the thickness or A specimen can be prepared by embedding a long sample vertically. The block support base 11A for slicing has the same external shape as that shown in FIG. 48, but the material for adhesive connection with the embedding agent is the same as that shown in FIG. 47. Molded with plastic or synthetic resin with a rough outer surface.

FIG. 43 is a block support base 11A for slicing having a certain thickness, there is no window at the bottom, sample number data recording paper 4 is pasted on the upper part of the recess, and an IC tag or the like around it. A data storage medium installation unit 30 is vertically formed. It is more convenient for the engaging part to attach the detachable lid part to the upper opening so as not to protrude to the outside of the block support base.
44 has the same thickness as the block support base 11A for slicing in FIG. 43, and has a window at the bottom, and a data storage medium installation section 30 such as an IC tag is vertically formed around the window. The sample number data recording paper 4 and the IC tag 37 may be affixed sideways in the recessed portion at the bottom of the block support base 11A for slicing.

  45 and 46 has a shape in which a ring (frame cassette) is inverted, and a window frame-like structure on the inner side of the embedding agent of the embedding agent paraffin 14. The biological sample 3 and the slicing block support base 11A are integrally fixed, held and embedded by tying an embedding agent in the lattice or structure inside. Therefore, the sample number data recording paper 4 and the data storage media setting part 30 such as an IC tag are adhered to the upper part and the bottom part of the concave part of the bottom of the window frame.

47, 48, and 49, there is no formable porous structure at the bottom of the block support base 11A for slicing, and the bottom is made of a porous material such as wooden / foamed plastic or a rough outer surface. 47 to 49, the bottom surface of the block support base 11A for slicing is placed on the support portion 13b, and the upper opening portion of the embedded frame-like base body 1 is molded. It is fitted inside.
The upper side of the bottom surface of the block support base 11A for slicing in FIG. 47 is formed in a box-like concave shape, and in the concave portion there is a sample number data recording paper 4 and a data storage media setting part 30 such as an IC tag. In FIG. 48, the recess of FIG. 47 is not molded.

FIGS. 50 and 51 show the block support base 11A for slicing in the upper opening of the embedding dish-like instrument made of the embedding frame-like base body 1 and the dish-like instrument 33a shown in FIG. The biological sample 3 and the slicing block support base 11A are integrally embedded by replenishing a molten embedding agent (paraffin) from the embedding center embedding agent injection port 36, and The sample number data recording paper 4 and the IC tag on which the sample number 5 and the barcode 4a are printed and described are placed on the porous bottom portion 11D of the block support base 11A, and are integrated by the solidified adhesive force of the embedding agent.
FIG. 52 shows the thin-cut block sample 51 that has been embedded in FIGS. 50 and 51, and the thin-cut block support base 11A is fixed to the cassette of the conventional thin-cut microtome adapter 31 by the thickness adjusting plate 44. It is the figure fixed according to the position of the fixed nail | claw of the apparatus 31a, and slicing.

  Further, in FIGS. 53 to 59, since the block support base 11A for slicing is no longer required to be used as a chemical treatment vessel for dehydration, degreasing, penetration, penetration of embedding agent, etc. When thinning from 1mm to 2mm or increasing the diameter of the pores at the bottom of the perforation, it is made into a lattice, or conversely, it is familiar with the embedding agent and finishes with surface processing with good adhesion and fine grained fine pores. Then, the block support base 11A having no large porous bottom surface can be formed. In addition, since it is no longer necessary to immerse the block support base 1 in a chemical solution, it is possible to print and record data storage media such as sample number 5 and bar code in advance. The block support base embedded from the beginning inside the cutting block support base 11A can be molded and used, and this is shown in the figure.

Data recording process:
FIG. 60 to FIG. 63 show that the sample number 5 and the bar are formed on the data recording unit 11a of the block support base 11A for slicing by engraving the surface by laser beam irradiation from the laser irradiation unit 48 of the laser engraving printing machine. It is explanatory drawing which is printing and printing the data storage media 4a, such as a code | cord | chord.
(A), (B), (C), (D), and (E) of FIG. 60 are recorded on the data recording portion 11a of the conventional thin-cut block support base 11A of the so-called standard-shaped cassette base type. It is the figure which carved and burned.
FIG. 61 (A) is a box-shaped type, and FIG. 65 (B) is a diagram showing the ring (frame cassette) type engraving and burning into the data recording portion 11a of the block support base 11A for slicing.

  FIG. 62 shows a sample number 5 or bar code by engraving the surface of the thin block support base 11A shown in FIGS. 53 to 60, in particular, the data recording unit 11a shown in FIG. 54 by laser beam irradiation. It is the figure which engraves and burns (FIG. 62 (A)) and the sample number 5 two-dimensional data code (FIG. 62 (B)).

FIG. 63 is a diagram in which the data recording section 11a of the block support base 11A for slicing is engraved and burned with the sample number 5 and the data storage medium 4a such as a bar code by the laser irradiation section 48 of the laser engraving and printing machine.
FIG. 63 (A) shows the block support base surface covering vapor deposition member 49b engraved with a laser to expose the block support base engraving adhesion developing member 49a, and print out the sample number 5 and the data storage medium 4a such as a bar code. 63 (B) is a surface explanatory view thereof.

  Furthermore, as described above, the pathological sample collection transfer acceptance verification cutout specimen preparation system according to the present invention is greatly different from the conventional Miles (US corporation) cassette system. Since the so-called cassette and embedding dish system process, which also serves as a base, was fundamental, the use of the Miles-type system instrument started from the time of trimming and cutting, which was the on-site work for pathological specimen preparation. Since the trimmed sample also serves as a chemical treatment vessel, it requires a certain volume or more, and data must be described as the block support base at the time of cutting. On the other hand, the thin-cut block support base 11A of the present invention does not absolutely require a storage section, and even if it is present, data storage medium 4a such as sample number data recording paper 4 or barcode, data storage such as IC tag, etc. It is sufficient to have a recess (installation section 30) with a depth of about 1 mm for storing media and the like, but the strong inside of the embedded frame-like base body 7 stored together with the patient sample in the recess. Sample number data recording paper 4 or bar code data storage medium 4a made of thin Kent paper or Western paper When the recording paper is taken out and spread flat and solidified and fixed integrally by the solidification adhesive force of the embedding agent such as paraffin, at that time As shown in FIG. 64, the data storage medium can be mechanically read by a reader (reader) even if the patient number and sample data are not printed on the block support base 11A for slicing. Readable slicing block sample 51 over data is completed.

It is a perspective view which shows embodiment of the embedding frame-shaped base | substrate basket | basket (container) of this invention. 2A is a plan view, and FIG. 2B is a longitudinal sectional view. It is a top view which shows other embodiment of the embedding frame-shaped base | basket | basket | basket (container) of FIG. It is a top view which shows other embodiment of the embedding frame-shaped base | basket | basket | basket (container) of FIG. FIG. 5 (A) is a longitudinal sectional view showing another embodiment of the embedded frame-shaped substrate bag (container) of FIG. 3, and FIG. 5 (B) is a side view of FIG. 5 (A). 6A is a plan view of FIG. 5A, and FIG. 6B is a front view of FIG. 5B. FIG. 7 is a slope view of FIG. 8A, FIG. 8B, and FIG. 8C are longitudinal sections of an embodiment different from the embodiment of FIG. It is the figure which removed the upper lid part of the embedding frame-like base | substrate board | substrate of this invention, and transferred the embedding frame-like base | substrate on a metal flat plate, and has constructed the embedding dish-like instrument. It is the figure which has moved the biological sample on the bottom metal plate of the embedding dish-shaped instrument constructed | assembled in FIG. FIG. 11A is a longitudinal sectional view showing a contact portion between the lower opening of the embedding frame-like substrate of the embedding dish-like instrument constructed in FIG. 9 and the metal flat plate, and FIG. 11B is FIG. ) Is an enlarged view of the main part of FIG. It is the figure which made the block support base fit to the upper side opening part of the embedding dish-shaped instrument of FIG. 11, FIG. 12 (A) is a top view, FIG. 12 (B) is a longitudinal cross-sectional view. FIG. 13A is a plan view and FIG. 13B is a longitudinal sectional view of the next stage work of the embedding work of FIG. FIG. 14 is an image diagram of various IC tags used in FIG. 13, FIGS. 14A, 14B, 14C, and 14D are plan views, and FIGS. 14A, 14B, 14C, and 14D are side views. . It is. It is the figure which showed the installation site | part of the IC tag shown in FIG. FIG. 16A is a view in which a sample number data recording paper and an IC tag are installed in the next embedding operation of FIG. 15, FIG. 16A is a plan view, and FIG. 16B is a longitudinal sectional view. It is the figure which has removed the block sample for slices in which the embedding work was completed in FIG. 16 from the embedding frame-shaped base | substrate. It is sectional drawing of the block sample for thin slices which is a part removed in FIG. FIG. 19A is a plan view, FIG. 19B is a vertical cross-sectional view, and FIG. 19C is FIG. It is a longitudinal cross-sectional view of a block sample for slicing different in shape from B). FIG. 20A is a plan view, and FIG. 20B is a longitudinal sectional view of an embedded frame-shaped base rod slightly different from FIG. 9 removes the upper lid portion of the embedded frame-shaped substrate basket different from the embedded frame-shaped substrate basket in FIG. 9, and transfers the embedded frame-shaped substrate onto the dish-shaped device to construct an embedded dish-shaped device. FIG. It is the figure which is performing the next work of FIG. It is the figure which is performing the operation | work following FIG. The shapes of the right and left thin block support bases are slightly different. FIG. 24A is a diagram in which the next operation of FIG. 23 is performed. FIG. 24B is a view in which the block sample for slicing is removed from the embedded frame-shaped substrate. FIG. 25A is a plan view and FIG. 25B is a longitudinal sectional view showing a popular type of block support base for slicing used in the present invention. FIG. 26A is a plan view, and FIG. 26B is a longitudinal sectional view of another example of FIG. FIG. 27A is a plan view of another example of FIG. 26, and FIG. 27B is a longitudinal sectional view thereof. FIG. 28A is a plan view, and FIG. 28B is a longitudinal sectional view, showing an example of another embodiment of FIG. FIG. 29A is a plan view and FIG. 29B is a longitudinal sectional view of an example of another embodiment of FIG. FIG. 30A is a plan view, and FIG. 30B is a longitudinal sectional view of another example of FIG. 29. FIG. 31A is a back view and FIG. 30B is a plan view of another example of FIG. It is an example of the sectional side view of FIG. It is a figure of the embedding operation | work using the part of FIG. FIG. 34 (A) is a view showing the relationship between the block support base for slicing and the embedding agent fitted into the upper opening of the embedding dish-like instrument made from the embedding frame-like substrate of the present invention. FIG. 34 (B) is a longitudinal sectional view of the embedded block sample embedded in FIG. 34 (A). FIG. 35 is a relationship diagram of an embodiment different from FIG. 34 of a block support base for slicing and an embedding agent that fits into an upper opening of an embedding dish-like instrument made from an embedding frame-like substrate. FIG. 36 is a relationship diagram of an embodiment different from FIG. 35 of a block support base for slicing and an embedding agent that fits into an upper opening of an embedding dish-like instrument made from an embedding frame-like substrate. FIG. 37 is a relationship diagram of an embodiment different from FIG. 36 showing a block support base for slicing fitted in an upper opening of an embedding dish-like instrument made from an embedding frame-like substrate and an embedding agent. FIG. 38 is a relationship diagram of an embodiment different from FIG. 37 of a block support base for slicing fitted in an upper opening of an embedding dish-like instrument made from an embedding frame-like substrate and an embedding agent. FIG. 39 is a relationship diagram of an embodiment different from FIG. 38 showing a block support base for slicing fitted in an upper opening of an embedding dish-like instrument made of an embedding frame-like substrate and an embedding agent. FIG. 40 is a relationship diagram of an embodiment different from FIG. 39 showing a block support base for slicing fitted in an upper opening of an embedding dish-like instrument made from an embedding frame-like substrate and an embedding agent. FIG. 41 is a relationship diagram of an embodiment different from FIG. 40 of the block support base for slicing and the embedding agent that fits into the upper opening of the embedding dish-like instrument made from the embedding frame-like substrate. FIG. 42 (A) shows an embodiment different from FIG. 34 of the block support base for slicing and the embedding agent fitted into the upper opening of the embedding dish-like instrument made from the embedding frame-like base. It is a relationship diagram. FIG. 42 (B) is a longitudinal sectional view of the embedded block sample embedded in FIG. 42 (A). FIG. 42 is a relationship diagram of an embodiment different from FIG. 42 showing the block support base for slicing and the embedding agent fitted into the upper opening of the embedding dish-like instrument made from the embedding frame-like base. FIG. 44 is a relationship diagram of another embodiment different from FIG. 43 of the block support base for slicing and the embedding agent fitted into the upper opening of the embedding dish-like instrument made from the embedding frame-like base. FIG. 45 is a relationship diagram of an embodiment different from FIG. 44 showing a block support base for slicing fitted in an upper opening of an embedding dish-like instrument made from an embedding frame-like substrate and an embedding agent. FIG. 46 is a relationship diagram of another embodiment different from FIG. 45 of the block support base for slicing fitted in the upper opening of the embedding dish-like instrument made from the embedding frame-like base and the embedding agent. FIG. 47 is a relationship diagram of an embodiment different from FIG. 46 of the block support base for slicing and the embedding agent fitted into the upper opening of the embedding dish-like instrument made from the embedding frame-like base. FIG. 48 is a relationship diagram of an embodiment different from FIG. 47 of the block support base for slicing and the embedding agent fitted into the upper opening of the embedding dish-like instrument made from the embedding frame-like base. FIG. 49 is a relationship diagram of an embodiment different from FIG. 48 of the block support base for slicing and the embedding agent fitted into the upper opening of the embedding dish-like instrument made from the embedding frame-like base. It is a top view of the embedding operation | work of FIG. It is a figure which is additionally replenished with the melt embedding agent in the embedding operation of FIG. FIG. 52 is a diagram in which an embedded block sample formed by the embedding operation in FIG. 51 is fixed to an adapter and sliced. FIG. 53A is a back view, FIG. 53B is a longitudinal sectional view, FIG. 53C is a transverse sectional view, and FIG. Fig. 5 is an example of data media installation of an embedded block sample in the surface view. It is a figure which shows the other Example of 11 A of thin block support bases of the part of FIG. 54A is a back view, FIG. 54B is a vertical cross-sectional view, FIG. 54C is a cross-sectional view, and FIG. 54D is a front view, showing an example of data medium placement of an embedded block sample. It is a figure which shows the other Example of 11 A of thin block support bases of the part of FIG. FIG. 55A is a back view, FIG. 55B is a longitudinal sectional view, FIG. 55C is a transverse sectional view, and FIG. 55D is a front view, which is an example of setting a data medium of an embedded block sample. It is a figure which shows the other Example of 11 A of thin block support bases of the part of FIG. 56A is a back view, FIG. 56B is a longitudinal cross-sectional view, FIG. 56C is a cross-sectional view, and FIG. 56D is a front view, showing an example of setting a data medium of an embedded block sample. It is a figure which shows the other Example of 11 A of thin block support bases of the part of FIG. 57A is a back view, FIG. 57B is a vertical cross-sectional view, FIG. 57C is a cross-sectional view, and FIG. 57D is a front view, showing an example of setting a data medium for an embedded block sample. It is a figure which shows the other Example of 11 A of thin block support bases of the part of FIG. 58A is a rear view, FIG. 58B is a longitudinal sectional view, FIG. 58C is a transverse sectional view, and FIG. 58D is a front view, which is an example of setting a data medium for an embedded block sample. It is a figure which shows the other Example of 11 A of thin block support bases of the part of FIG. 59A is a back view, FIG. 59B is a longitudinal cross-sectional view, FIG. 59C is a cross-sectional view, and FIG. 59D is a front view, showing an example of setting a data medium for an embedded block sample. FIG. 60A is a front view (data recording unit 11a) side, and FIG. 60B is a perforated bottom plate 11B. FIG. 60C is a view seen from the plan view side of the block support base 11 </ b> A having the shape. 60 (D) and 60 (E) show a data storage medium such as a sample number 5 and a bar code engraved on the data recording portion 11a on the side view side of FIGS. 60 (A) and 60 (B). It is a figure. It is the figure which carved the data storage media, such as a sample number 5 and a barcode, with the laser beam in the data recording part 11a of another embedding block support base 11A of FIG. 60. 61A is a front view of a box type, and FIG. 61B is a plan view of a ring (frame cassette) type. FIG. 60 is a diagram in which a data storage medium such as a sample number and a bar code is engraved and burned in a data recording portion of another embedding block support base 11A different from FIGS. 60 and 61 with a laser beam. FIG. 60A is a plan view in which a sample number and a barcode are engraved, and FIG. 60B is a plan view in which a sample number and a two-dimensional data code are engraved. FIG. 63A is a front view, in which a data storage medium such as a sample number and a bar code is engraved with a laser beam on the data recording portion of the embedding block support base 11A for thin slicing of FIG. 60 to FIG. FIG. 63B is a plan view.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Embedding frame-shaped base | substrate 1A Opening part 2 Cover part 3 Trimmed biological specimen 4 Sample number etc. data recording paper 7 Embedding frame-shaped base | substrate board | substrate 11A Block support base 11B Block support base porous bottom plate 11C Block support base Opening part 11D Block support base porous bottom part 14 Embedding agent 30 IC tag etc. data storage media installation part 33 Metal flat plate 33a Dish instrument 36 Embedding center embedding agent inlet 37 Striped IC tag 38 Label type IC tag 39 Coin type IC tag 40 Sesame grain IC tag 48 Laser engraving printing machine irradiation part 49a Block support base carving adhesion developing member 49b Block support base surface covering member 51 Block sample for slicing

Claims (13)

An embedding frame-shaped base body provided with a cylindrical embedding frame-shaped base body having openings on the upper side and the lower side;
A chemical solution treatment basket is formed by providing a lid cover detachably at the upper and lower openings of the embedded frame-shaped substrate,
An embedding instrument is formed by sealing a lower opening of the embedding frame base with a bottom plate, and a block support base for slicing is attached to the upper opening of the embedding frame base. basket.   2. The embedding frame-shaped substrate according to claim 1, wherein the block support base for slicing is a lid for a block support base, and the bottom plate is a metal flat plate or an embedding dish. basket.   Sample storage and transfer with dehydration, degreasing, penetration, embedding agent penetration, with openings on the upper and lower sides, and jar lids that are porous or have a large number of liquid passage holes in the openings. An embedding frame-shaped base body that combines the function of the outer frame, which is a part of the embedding device, and the chemical treatment container such as the above.   A biological sample is stored in a space in which an opening is formed on the upper side and the lower side, and a gutter lid portion in which the opening or a plurality of liquid passage holes are formed is detachable, and the sample is stored. With the frame-shaped base plate, the thin-cut block support base, which is separately prepared in the upper opening, is fitted on the frame-type base plate that can perform chemical processing such as dehydration, degreasing, penetration, and embedding agent penetration. The embedding frame-like substrate bag according to claim 3, which can be made.   An opening is formed on the upper and lower sides, and a jar lid with a porous or many liquid passage holes is formed in the opening so as to be detachable, and a biological sample is stored to dehydrate, degrease, penetrate, and embed Remove the lid of the upper and lower openings of the frame-shaped base plate that has been subjected to chemical treatment such as agent permeation, and remove the bottom of the frame-shaped base plate without the cover portion from a metal plate or dish with good thermal conductivity such as stainless steel 5 or 4 that can also be used as an embedding dish-like instrument formed by cooling and solidifying an embedding agent such as paraffin adhering to the outer periphery of the bottom of the frame-shaped substrate. The embedded frame-like substrate bag as described.   An opening is formed on the upper and lower sides, and a jar lid with a porous or many liquid passage holes is formed in the opening so as to be detachable, and a biological sample is stored to dehydrate, degrease, penetrate, and embed Remove the lid of the upper and lower openings of the frame-shaped base plate that has been subjected to chemical treatment such as agent permeation, and remove the bottom of the frame-shaped base plate without the cover portion from a metal plate or dish with good thermal conductivity such as stainless steel The biological sample is transferred from the top of the opening to the bottom of the embedding dish-like instrument formed by cooling and solidifying the embedding agent such as paraffin adhering to the outer periphery of the bottom of the frame-shaped substrate. Inject and replenish the same embedding agent, place and fit a separately prepared block support base for thin cutting, and integrate the sample and base with the embedding agent such as paraffin in one piece with the embedding agent such as paraffin It can be used as a solid solution fixed sample chemical treatment bowl and as an outer frame base for embedding dishes. That claims 3 and 4, or, 5 wherein the embedding frame-like base body cage.   3. The embedded frame-shaped substrate body according to claim 2, wherein the thin-cut block support base or the lid cover part for the thin-cut block support base is provided with data recording means.   A data recording means is also used for the lid portion of the frame-shaped substrate tub that stores biological samples and performs chemical processing such as dehydration, degreasing, penetration, embedding agent penetration, etc. The predetermined data recording part of the block support base for slicing is engraved with a laser beam in the predetermined data recording part of the block support base for slicing, and the attributes, sample numbers, barcodes and two-dimensional data codes of the biological samples are engraved In order to surely describe the data storage medium, etc., only the data recording part of the block support base for slicing or the entire surface of the support base is engraved by the laser engraving and printing function unit Alternatively, it is formed into a two-layer structure consisting of a surface coating member such as plating or aluminum foil coating vapor deposition and a lower engraved adhesion developing member, and a barcode, two-dimensional data code, etc. of the engraved part is formed. According to claim 7, wherein embedding the frame-shaped body cage, characterized in that the data storage medium and characters are formed on the structure that can clearly emerge reliably.   An opening is formed on the upper and lower sides, and a jar lid with a porous or many liquid passage holes is formed in the opening so as to be detachable, and a biological sample is stored to dehydrate, degrease, penetrate, and embed Remove the lid of the upper and lower openings of the frame-shaped base plate that has been subjected to chemical treatment such as agent permeation, and remove the bottom of the frame-shaped base plate without the cover portion from a metal plate or dish with good thermal conductivity such as stainless steel The biological sample placed on the lid of the lower opening of the frame-like substrate is transferred to the bottom of the storage of the embedding dish-like instrument placed on the shaped instrument, and embedded in an embedding agent such as paraffin. An embedding method in which the thin-cut block support base is solidified and fixed integrally with the upper opening of the frame-like base constituting the shape function of the embedding dish-like instrument.   An opening is formed on the upper and lower sides, and a jar lid with a porous or many liquid passage holes is formed in the opening so as to be detachable, and a biological sample is stored to dehydrate, degrease, penetrate, and embed Remove the lid of the upper and lower openings of the frame-shaped base plate that has been subjected to chemical treatment such as agent permeation, and remove the bottom of the frame-shaped base plate without the cover portion from a metal plate or dish with good thermal conductivity such as stainless steel The biological sample placed on the lid of the lower opening of the frame-like substrate is transferred to the bottom of the storage of the embedding dish-like instrument placed on the shaped instrument, and embedded in an embedding agent such as paraffin. , The attribute of the sample and the sample at a predetermined location that does not get in the way when slicing the block support base for slicing mounted on the upper opening of the frame-shaped base constituting the shape function of the embedding dish-like instrument Data storage media that can be printed on paper labels such as numbers, barcodes, and two-dimensional data codes Embedding method according to claim 9, wherein the attributes and the sample data can be reliably reconciliation solidification adhesion paraffin embedding agent to prevent contamination or loss of the sample solidifies fixed integrally.   An opening is formed on the upper and lower sides, and a jar lid with a porous or many liquid passage holes is formed in the opening so as to be detachable, and a biological sample is stored to dehydrate, degrease, penetrate, and embed Remove the lid of the upper and lower openings of the frame-shaped base plate that has been subjected to chemical treatment such as agent permeation, and remove the bottom of the frame-shaped base plate without the cover portion from a metal plate or dish with good thermal conductivity such as stainless steel The biological sample placed on the lid of the lower opening of the frame-like substrate is transferred to the bottom of the storage of the embedding dish-like instrument placed on the shaped instrument, and embedded in an embedding agent such as paraffin. In the thin-cut block support base that is placed on and fitted into the upper opening of the frame-shaped base constituting the shape function of the embedding dish-like instrument, the thin-cut block during the chemical solution treatment step of the biological sample In the data recording part of the support base, the attribute, sample number, barcode, and two-dimensional data code of the sample In addition, a data storage medium that is weak against water, acids, organic solvents, etc. such as an IC tag is placed on the block support base for each thin slice. The embedding method according to claim 9 or 10, wherein the embedding method is bonded and housed in the housing portion of the block support base for slicing when the embedding operation is completed, and is solidified and fixed integrally with the wireless IC tag.   An opening is formed on the upper side and the lower side, and a lid part having a porous or a large number of liquid passage holes is formed in the lower side opening so as to be detachable. A block support base for slicing formed with a bottom or a plurality of liquid passage holes is placed and fitted, and a biological sample is stored in the formed space to dehydrate, degrease, penetrate, and embed Remove the lid on the lower opening of the frame-shaped base plate that has been subjected to chemical treatment such as agent permeation and remove the biological sample placed on the lid of the lower side of the frame-shaped base plate with heat from stainless steel or the like. Move to a metal dish-like instrument with good conductivity, place the frame-like base on which the lid of the lower opening is removed on the metal dish-like instrument, and place the storage bottom of the embedding dish-like instrument A biological sample formed at the bottom of the opening on the lower side of the frame-shaped substrate without the lid cover is embedded in an embedding agent such as paraffin and embedded. The thin-cut block support base and the biological sample which are placed and fitted in the upper opening of the frame-shaped base constituting the shape function of the dish-shaped instrument are integrally solidified and fixed. The embedding method described. An embedding method using the embedding frame-like substrate bag according to claim 1, wherein:
A process for attaching a lid to the upper and lower openings of the embedding frame-shaped substrate to form a medicinal solution treated soot, and storing a biological sample in the medicinal solution treated soot;
A step of performing chemical treatment by immersing the chemical treatment rod in a chemical solution;
Removing the upper and lower lid portions of the chemical treatment rod, and sealing the lower opening of the embedded frame base with a bottom plate to form an embedding instrument;
Placing the chemical-treated biological sample on the bottom plate of the embedding instrument, and mounting a block support base for slicing on the upper opening;
An embedding method comprising:

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