JP3433129B2 - Frame cassette for histopathological examination - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a histopathological examination frame cassette used for producing a microscopic specimen for pathological tissue examination and cryopreservation of the specimen, and more specifically, to a microtome during microscopic specimen production. The present invention relates to a pathological tissue inspection frame cassette for preventing a rise from an attached state and manufacturing a predetermined number of specimens with a uniform thickness.

[0002]

2. Description of the Related Art Conventionally, as shown in FIGS. 22 and 23, a frame cassette of this type has a peripheral wall portion 1 and a projecting edge portion 2 which are made of a synthetic resin having chemical resistance and low temperature resistance. It will be. The peripheral wall portion 1 is a rectangular container having an open upper surface, and has a large number of pores 3 or reinforcing ribs 4 formed in the bottom portion. The projecting edge portion 2 is a plate-shaped body and extends outward from the facing side of the bottom portion of the peripheral wall portion 1.

In order to prepare a microscope sample using the frame cassette, in the case of the frame cassette shown in FIG. 22, first, as shown in FIG. 24, the collected sample 5 is washed with water, and the sample 5 is washed with alcohol. Water is removed, and xylene imparts an affinity with the liquid paraffin 7 described later.

Next, as shown in FIG. 25, the liquid paraffin 7 is put in the stainless tray 6, the sample 5 is taken out from the peripheral wall portion 1 and placed on the liquid paraffin 7. continue,
Place the frame cassette on the stepped portion of the tray 6, and until the bottom of the peripheral wall 1 is submerged in the liquid paraffin 7,
Add to sample 5. The step portion of the tray 6 is formed in a square shape so that the projecting edge portion 2 is inserted therein. After the liquid paraffin 7 is solidified, the tray 5 is removed to remove the sample 5
A cassette block 8 (see FIG. 26) is obtained in which the paraffin 7 having embedded therein is attached to the bottom portion of the peripheral wall portion 1.

Next, as shown in FIGS. 26 and 27, the cassette block 8 is placed upside down on the base 11 of the adapter 10 mounted on the microtome 9, and the L-shaped cross section is erected on the base 11. The peripheral wall portion 1 is fitted between two shaped concave projections 12 and the peripheral wall portion 1 is sandwiched between the fixed arm 14 and the movable arm 15 provided on the base 11 by operating the lever 13. And fix it. Subsequently, a portion of the paraffin 7 in which the specimen 5 is embedded is sliced in the direction of arrow a or b in FIG. 27, the sliced slice is stained, and other predetermined processing is performed to obtain a microscope sample.

When the specimen 5 is stored in a frozen state, as shown in FIG. 25, it is stored in a predetermined storage temperature by being immersed in liquid nitrogen as it is before the tray 6 is removed and frozen.

In the case of the frame cassette shown in FIG. 23, although not shown, for example, as described in Japanese Utility Model Publication No. 3-55090 and Japanese Utility Model Publication No. 5-40449, a sample storage chamber having a pore at the bottom is provided. One or a plurality of inspection cassettes are attached to the upper open portion of the frame cassette with the lid removed or opened, and the same washing as above, alcohol, xylene, and liquid paraffin pouring is performed in the inspection storage chamber. The cassette block is obtained by performing additional operations. Even in the case of the frame cassette shown in FIG. 22, the cassette block can be obtained by attaching the above-mentioned inspection cassette to the open upper surface and performing operations such as washing with water.

[0008]

However, when the cassette block 8 using the conventional frame cassette is sliced into thin pieces to prepare a microscope sample, the adapter 1 is used.
Since the frictional force between the fixed arm 14 and the movable arm 15 of 0 and the peripheral wall portion 1 of the frame cassette is not so large, the peripheral wall portion 1 slides upward during the slicing, and the cassette block 8
May rise from the base 11. If the cassette block 8 floats up, there is a problem in that the thickness of the microscope sample becomes non-uniform, and as a result, it becomes impossible to obtain a predetermined number of microscope samples. The present invention is
It is an object of the present invention to improve a conventional frame cassette and solve such a problem.

[0009]

In order to achieve the above object, a pathological tissue inspection frame cassette of the present invention comprises a peripheral wall portion and a projecting edge portion made of a synthetic resin having chemical resistance and low temperature resistance. The peripheral wall has a square shape with an open upper surface,
While having a large number of pores or reinforcing ribs in the bottom, the projecting edge is a plate-like body, which is extended outward from the opposite side of the bottom of the peripheral wall, paraffin with a specimen embedded in the bottom. For histopathological examination to obtain the microscope specimen by inverting the mounted cassette block on the base of the microtome, sandwiching the peripheral wall between the dents and slicing paraffin embedded with the specimen In the frame cassette, a plurality of lateral ridges or grooves or embossed surfaces such as a satin finish are formed on the outer periphery of the peripheral wall portion as a means for preventing the frame cassette from floating from the mounted state on the microtome. It is characterized by that.

Further, it is preferable that the lifting preventing means is composed of a plurality of lateral protrusions or grooves, or an embossed surface such as a satin finish. Further, it is preferable that the peripheral wall portion is tapered in an inverted pyramid shape. Further, it is convenient to form a recording portion for recording the name and the like of the subject on the protruding edge portion.

[0011]

When a cassette block is manufactured by using the frame cassette of the present invention having the above-mentioned structure, the cassette block is surely prevented from being lifted up when the cassette block is attached to the microtome and sliced into thin pieces to prepare a microscope sample. can do.

As a preferred embodiment of the lifting preventing means, in the case where a plurality of lateral ridges or grooves are formed on the outer periphery of the peripheral wall portion of the frame cassette, the fixed arm and the movable arm of the adapter mounted on the microtome are The tip portion engages so as to bite into the space between the protrusions or the groove to reliably prevent the cassette block from rising.

As another preferred embodiment, in the case where an embossed surface such as a satin finish is formed on the outer periphery of the peripheral wall of the frame cassette, the fixed claws of the fixed arm and the movable arm of the adapter and the embossed surface are formed. Since the frictional force between the cassette block and the surface is large, there is no risk that the peripheral wall will slide upward and the cassette block will rise.

Further, in the case where the peripheral wall of the frame cassette is tapered in the shape of an inverted pyramid, the cassette block is mounted inside out on the base of the adapter during slicing, so the taper of the peripheral wall is a forward pyramid. Become a state. For this reason, the fixed claws of the adapter and the locking claws of the movable arm press the peripheral wall portion downward to prevent the cassette block from rising. Therefore, according to the present invention, the problem that the sample thickness becomes non-uniform due to the lifting of the cassette block during the preparation of the microscope sample and the predetermined number of samples cannot be obtained due to the increase in thickness is solved, and the efficiency is improved. A microscope sample can be produced well.

[0015]

Embodiments of the present invention will be described below with reference to the drawings, but it goes without saying that the present invention is not necessarily limited to these embodiments. Note that the same reference numerals are used for the same portions or the same elements in each drawing.

Embodiment 1 As shown in FIGS. 1 to 4, a frame cassette 20A of this embodiment comprises a peripheral wall portion 21 and a projecting edge portion 22 made of a synthetic resin having chemical resistance and low temperature resistance. It will be done. As the synthetic resin, polyacetal, polycarbonate and the like are suitable.

The peripheral wall portion 21 is a rectangular container having an open upper surface, has a large number of pores 23 in the bottom portion, is provided with an inverted pyramidal taper 24 on the outer periphery, and has a plurality of lateral surfaces on four sides. The ridge 25 is formed. Although not shown, a groove may be formed instead of the protrusion 25. The ridge portion 22 is a plate-like member and extends outward from the opposite side of the bottom portion of the peripheral wall portion 21, and has satin-like recording portions 26 formed on both front and back surfaces of both ends. The name and the like of the subject are recorded in the recording unit 26.

In order to prepare a microscope sample using the frame cassette 20A having the above-described structure, first, as shown in FIG. 5, the sample 27 collected is housed in the peripheral wall portion 21 and washed with water, and the sample 27 is washed with alcohol. Water is removed, and xylene imparts an affinity with the liquid paraffin 29 described later.

Next, as shown in FIG. 6, the liquid paraffin 29 is put in the stainless tray 28, the sample 27 is taken out from the peripheral wall portion 21 and placed on the liquid paraffin 29.
Subsequently, the frame cassette 20A is placed on the stepped portion of the tray 28, and the liquid paraffin 29 is poured over the sample 27 until the bottom of the peripheral wall portion 21 is immersed in the liquid paraffin 29. The step portion of the tray 28 is formed in a square shape so that the projecting edge portion 22 is inserted.

Tray 2 after liquid paraffin 29 is solidified
By removing 8 the paraffin 29 in which the specimen 27 is embedded adheres to the bottom of the peripheral wall 21 to obtain a cassette block 30 (see FIG. 7).

Next, as shown in FIGS. 7 and 8, the cassette block 30 is placed upside down on the base 33 of the adapter 32 mounted on the microtome 31, and the L-shaped cross section is erected on the base 33. The peripheral wall portion 21 is fitted between the two shaped concave projections 34, and the peripheral wall portion 21 is sandwiched between the fixed arm 36 and the movable arm 37 provided on the base 33 by operating the lever 35. And fix it. At that time, both arms 3
The locking claws 38 formed at the tips of the reference numerals 6 and 37 engage so as to bite between the lateral projections 25 formed on the peripheral wall portion 21. At the same time, since the cassette block 30 is mounted inside out, the taper 24 on the outer periphery of the peripheral wall portion 21 has a forward pyramid shape. Therefore, the locking claw 38 is provided on the peripheral wall portion 2.
1 is pressed downward. As a result, both arms 3
The locking claws 38 of 6 and 37 are used for the cassette block 3 described later.
The cassette block 30 is reliably prevented from rising from the base 33 when slicing 0.

Subsequently, a portion of the paraffin 29 in which the specimen 27 is embedded is sliced in the direction of arrow A or B in FIG. 8, and the sliced slice is stained and other predetermined processing is performed to obtain a microscope sample.

When the specimen 27 is frozen and stored,
As shown in FIG. 6, the tray 28 is immersed in liquid nitrogen as it is before being removed, frozen, and stored at a predetermined storage temperature.

Embodiment 2 As shown in FIGS. 9 to 11, the structure of the frame cassette 20B of this embodiment is the same as that of Embodiment 1 except that the reinforcing ribs 39 are provided on the bottom of the peripheral wall portion 21 in a cross beam shape. 1)). Therefore, the protrusion 25 and the taper 24 of the peripheral wall portion 21 act in the same manner as in the first embodiment, and prevent the cassette block using the frame cassette 20B from rising.

Embodiment 3 As shown in FIG. 12, a frame cassette 20 of this embodiment
The configuration of C is the same as that of the second embodiment (FIG. 9) except that a plurality of continuous lateral projections 25 are formed on the entire outer peripheral surface of the peripheral wall portion 21.
See). Therefore, the protrusion 25 and the taper 24 of the peripheral wall portion 21 act in the same manner as in the first embodiment, and prevent the cassette block using the frame cassette 20C from rising.

Embodiment 4 As shown in FIG. 13, a frame cassette 20 of this embodiment
In the configuration of D, a plurality of lateral ridges 25 are formed on the two opposing surfaces of the peripheral wall portion 21, and a matte embossed surface 40 is formed on the remaining two opposing surfaces. The same as Example 1 (see FIG. 1). Therefore,
The protrusion 25 and the taper 24 of the peripheral wall portion 21 are the same as those in the first embodiment.
The same operation is performed to prevent the cassette block using the frame cassette 20D from rising.

Although not shown, as another method of use, when the embossed surface 40 is sandwiched between the fixed arm 36 and the movable arm 37 of the adapter 32, both arms 3
A large frictional force is exerted between the locking claws 38 and 37 and the embossed surface 40. Therefore, the friction force and the taper 2
4 and the floating of the cassette block using the frame cassette 20D is prevented.

Embodiment 5 As shown in FIG. 14, a frame cassette 20 of this embodiment
The configuration of E is the same as that of the fourth embodiment (see FIG. 13) except that the reinforcing ribs 39 are provided on the bottom portion of the peripheral wall portion 21 in a cross beam shape. Therefore, the actions and effects of the protrusion 25 and the embossed surface 40 of this embodiment are not different from those of the fourth embodiment.

Embodiment 6 As shown in FIG. 15, a frame cassette 20 of this embodiment
The configuration of F is such that, instead of the lateral ridges 25 in the fifth embodiment (see FIG. 14), a plurality of laterally intermittent ridges 4 are provided.
Example 5 is the same as Example 5 except that No. 1 was formed. The ridges 41 obviously act in the same manner as the ridges 25 of the first embodiment (see FIG. 1). Therefore, the operation and effect of this embodiment are not different from those of the first embodiment.

Embodiment 7 As shown in FIG. 16, a frame cassette 20 of this embodiment
The configuration of G is the same as that of the fifth embodiment except that a plurality of rectangular ridges 42 are formed in a grid pattern instead of the lateral ridges 25 of the fifth embodiment (see FIG. 14). . When the cassette block using the frame cassette 20G is sandwiched between the fixed arm 36 and the movable arm 37 of the adapter 32, the engaging claws 38 of both arms 36 and 37 are engaged so as to bite between the rectangular protrusions 42. To meet. Therefore, the cassette block has a rectangular protrusion 42 as in the first embodiment.
Also, due to the action of the taper 24, lifting is prevented. In addition, the operation and effect of the embossed surface 40 is the same as that of the fourth embodiment.
Is not different.

Embodiment 8 As shown in FIG. 17, the frame cassette 20 of this embodiment
The configuration of H is the same as that of the second embodiment (see FIG. 9) except that the satin-shaped embossed surface 40 is formed on the four outer peripheral surfaces of the peripheral wall portion 21. The embossed surface 40 acts in the same manner as the embossed surface 40 in the fourth embodiment (see FIG. 13) and, in combination with the action of the taper 24, prevents the cassette block from rising using the frame cassette 20H. .

Embodiment 9 As shown in FIGS. 18 and 19, the construction of the frame cassette 20I of this embodiment is carried out except that the peripheral wall portion 21 is erected vertically to the projecting edge portion 22. This is the same as Example 2 (see FIG. 9). Therefore, the frame cassette 2
In the cassette block using 0I, Example 2
There is no action of the taper 24 as in the above, but the peripheral wall portion 2
The ridge 25 of No. 1 acts in the same manner as in the second embodiment, and prevents the cassette block from rising.

Embodiment 10 As shown in FIGS. 20 and 21, the structure of the frame cassette 20J of this embodiment is the same as that of the embodiment except that the outer periphery of the peripheral wall portion 21 has a forward pyramidal taper 43. 2 (see FIG. 9). Therefore, when the cassette block using the frame cassette 20J is turned upside down and attached to the adapter 32, the taper 43 becomes an inverted pyramid shape, and the taper 24 does not have the action as in the second embodiment. The stop claw 38 engages so as to bite between the protrusions 25 of the peripheral wall portion 21 to prevent the cassette block from rising.

[0034]

As described above, according to the present invention, the rising prevention means of the frame cassette cassette is formed on the outer periphery of the peripheral wall portion by the embossed surface such as the lateral protrusions or grooves or the satin finish. Problems such as non-uniformity of the thickness of the microscope sample and the inability to obtain a predetermined number of samples due to the increased thickness are prevented, and the microscope sample can be efficiently manufactured. When the peripheral wall is tapered in the shape of an inverted pyramid, the locking claws of the fixed arm and the movable arm of the adapter press the peripheral wall of the cassette block downward, so that the ridge or groove or emboss Combined with the processed surface, it has the effect of increasing the lifting prevention effect.

[Brief description of drawings]

FIG. 1 is a perspective view showing a frame cassette according to a first embodiment of the present invention.

FIG. 2 is a front view of the frame cassette of the first embodiment.

FIG. 3 is a plan view of FIG.

4 is a sectional view taken along line IV-IV in FIG.

FIG. 5 is a cross-sectional view showing a state in which a sample is processed by the frame cassette of the first embodiment.

FIG. 6 is an explanatory diagram showing a process of producing a microscope sample using the frame cassette of Example 1.

FIG. 7 is an explanatory diagram showing another process of producing a microscope sample using the frame cassette of Example 1.

8 is a partially cutaway plan view of FIG. 7. FIG.

FIG. 9 is a perspective view showing a frame cassette according to a second embodiment of the present invention.

FIG. 10 is a plan view of the frame cassette according to the second embodiment.

11 is a sectional view taken along line XI-XI of FIG.

FIG. 12 is a perspective view showing a frame cassette according to a third embodiment of the present invention.

FIG. 13 is a perspective view showing a frame cassette according to a fourth embodiment of the present invention.

FIG. 14 is a perspective view showing a frame cassette according to a fifth embodiment of the present invention.

FIG. 15 is a perspective view showing a frame cassette according to a sixth embodiment of the present invention.

FIG. 16 is a perspective view showing a frame cassette according to a seventh embodiment of the present invention.

FIG. 17 is a perspective view showing a frame cassette according to an eighth embodiment of the present invention.

FIG. 18 is a perspective view showing a frame cassette according to a ninth embodiment of the present invention.

FIG. 19 is a front view of the frame cassette according to the ninth embodiment.

FIG. 20 is a perspective view showing a frame cassette according to Example 10 of the present invention.

FIG. 21 is a front view of the frame cassette according to the tenth embodiment.

FIG. 22 is a perspective view showing an example of a conventional frame cassette.

FIG. 23 is a perspective view showing another example of a conventional frame cassette.

FIG. 24 is a cross-sectional view showing a state in which a sample is processed by a conventional frame cassette.

FIG. 25 is an explanatory diagram showing a process of producing a microscope sample using a conventional frame cassette.

FIG. 26 is an explanatory view showing another process of producing a microscope sample using a conventional frame cassette.

27 is a partially cutaway plan view of FIG. 26. FIG.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Peripheral wall part 2 Protrusion edge part 3 Pore 4 Reinforcing rib 5 Sample 6 Tray 7 Paraffin 8 Cassette block 9 Microtome 10 Adapter 11 Base 12 Gradient projection 13 Lever 14 Fixed arm 15 Movable arm 20A-J Frame cassette 21 Peripheral wall part 22 Projection edge 23 Pore 24 Taper 25 Projection 26 Recording section 27 Sample 28 Tray 29 Liquid paraffin 30 Cassette block 31 Microtome 32 Adapter 33 Base 34 Gradient projection 35 Lever 36 Fixed arm 37 Movable arm 38 Locking claw 39 Reinforcing rib 40 Embossed surface 41 Intermittent ridge 42 Square protrusion 43 Tapered

─────────────────────────────────────────────────── ─── Continuation of the front page (58) Fields surveyed (Int.Cl. ⁷ , DB name) G01N 1/00-1/44 G01N 33/48

Claims (3)

(57) [Claims] 1. A square wall having a peripheral wall portion made of a synthetic resin having chemical resistance and low temperature resistance and a projecting edge portion, the peripheral wall portion having a square upper surface, and a large number of pores or reinforcing ribs at the bottom portion. In addition, the projecting edge portion is a plate-like body, and is extended outward from the opposite side of the bottom portion of the peripheral wall portion, and a cassette block formed by attaching paraffin embedding a sample to the bottom portion of a microtome A histopathological examination frame cassette for obtaining a microscope specimen by slicing paraffin embedded with a specimen by sandwiching the peripheral wall portion between dendrites on a base, and the frame cassette is a microtome. As a means for preventing the rise from the state of being attached to a pathological tissue, characterized in that a plurality of lateral ridges or grooves or embossed surfaces such as satin finish are formed on the outer periphery of the peripheral wall portion. Inspection frame cassette. 2. The frame cassette for pathological tissue examination according to claim 1, wherein the peripheral wall portion is tapered in an inverted pyramid shape. 3. The recording portion is formed on the protruding edge portion.
The frame cassette for pathological tissue examination described.