CN107576558A - A kind of histopathology sample dehydration waxdip box for exempting from embedding and application method - Google Patents
A kind of histopathology sample dehydration waxdip box for exempting from embedding and application method Download PDFInfo
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- CN107576558A CN107576558A CN201710885242.7A CN201710885242A CN107576558A CN 107576558 A CN107576558 A CN 107576558A CN 201710885242 A CN201710885242 A CN 201710885242A CN 107576558 A CN107576558 A CN 107576558A
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- box
- waxdip
- box body
- exempting
- sample
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- 230000018044 dehydration Effects 0.000 title claims abstract description 33
- 238000006297 dehydration reaction Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000001575 pathological effect Effects 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000012188 paraffin wax Substances 0.000 claims description 10
- 239000001993 wax Substances 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 8
- 239000004575 stone Substances 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 229940057995 liquid paraffin Drugs 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 4
- DHKHKXVYLBGOIT-UHFFFAOYSA-N 1,1-Diethoxyethane Chemical compound CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 claims description 3
- 239000011354 acetal resin Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 229920006324 polyoxymethylene Polymers 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000009434 installation Methods 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 238000005406 washing Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 241000628997 Flos Species 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004018 waxing Methods 0.000 description 1
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Abstract
The invention provides a kind of histopathology sample dehydration waxdip box for exempting from embedding and application method, including box body, lid and base, and box body has been built into and has been placed in box space cavernous body of the same size, tissue specimen in the gap on the top of cavernous body.The outer wall of box body and the surface distributed of lid have open-work, the liquid of various processes is penetrated into by open-work in cavernous body.The cavernous body is provided with some tangent lines extended downwardly in upper surface, and pending sample is inserted in the gap that tangent line is formed, and sample can be strapped in the position of placement, and the liquid used in various processes can also pass through cavernous body and act on sample.The dehydration waxdip box of the present invention is after tissue specimen is put into, fixation, washing, dehydration, transparent and waxdip these steps can be carried out continuously without compartment of terrain, need not moving specimens, embedding effect during sample has reached conventional procedures during waxdip simultaneously, it can be achieved to exempt to embed, greatly improve the efficiency made in pathological section.
Description
Technical field
The present invention relates to technical field of medical instruments, and in particular to a kind of histopathology sample dehydration waxdip for exempting from embedding
Box and application method.
Background technology
Paraffin section is method most widely used in histology conven tional tabletting techniques.Paraffin section is applied not only to observe
The morphosis of normal cell tissue, and the subject such as pathology and medical jurisprudence is studying, observe and judge cell tissue
The main method of metamorphosis, and be quite widely used in the research of other many ambits.Prior paraffin is cut
Piece method includes materials, fixed, washing and dehydration, transparent, waxdip, embedding, section and paster, dewaxing, dyeing, dehydration, transparent, envelope
The steps such as piece.
Prior paraffin microtomy, it is by gathering representative histopathology sample（Hereinafter referred to as sample）, put
Enter in embedded box, using automatic machinery or manually by the embedded box equipped with sample successively by fixed, dehydration, transparent and waxdip
After these steps, clamp by hand（Usually use tweezers）Sample after waxdip is placed on equipped with wax liquor（Liquid paraffin）Mould in fit
Work as position, sample decontroled after the partially solidified tissue of wax liquor is essentially fixed in precalculated position, then embedded box is put into mould,
The partially submerged embedded box of wax liquor is added, the wax stone containing sample is made after wax solidification.
In the processing procedure of traditional sample, fixed, washing, dehydration, transparent, waxdip and embedding are must before sample is cut into slices
The step of must carrying out, multiple steps therein need for sample to be transferred to different platform progress, so between each step inherently
Having time interval, causes waste of time, extends the cycle of sample disposal.
The content of the invention
Technical problem of the present invention for solution in above-mentioned, there is provided a kind of can be realized in the processing procedure of sample exempts from embedding
Histopathology sample is dehydrated waxing device and application method.
The technical solution adopted in the present invention is：
A kind of histopathology sample dehydration waxdip box for exempting from embedding, it is characterised in that including：
Open-work is distributed with-box body, the outer wall of the box body, for loading and penetrating into corresponding liquid；
- lid, located at the cassette upper end, and provided with the clip for being used to be connected with box body, the lid surface distributed has above-mentioned
Box body identical open-work；
- base, it is integrally formed with the bottom of the box body, for coordinating the clamping mouth of waxdip box and tissue pathological slice machine
Connection；
- cavernous body, insert in the box body, and consistent with the space size in box body, the cavernous body in top be provided with it is some to
The tangent line of lower extension, pending sample are inserted in the gap that tangent line is formed；
The box body, lid and base select the material consistent with existing paraffin section embedded box, such as acetal resin.
Edge corresponding to the edge and the box body of the lid side connects to may be reversed.
The clip is buckle, is symmetrically set in the inner side of the lid, and the box body is provided with corresponding in correspondence position
Buttonhole.
The open-work is uniformly distributed in the outer wall of the box body and the lid surface, a diameter of 0.5-3mm of open-work,
Adjacent open-work spacing is 1-5mm.
The tangent line is set for transverse and longitudinal, forms the cavernous body upper surface latticed.
Parallel and adjacent to the tangent line spacing be 1-5mm.
A kind of application method for the histopathology sample dehydration waxdip box for exempting from embedding, comprises the following steps：
S1:Sample is placed in the gap that cavernous body top tangent line is formed, puts down the upper surface for treating section and cavernous body of sample
Together；
S2：According to sample processing steps, the reagent needed for fixation, dehydration, transparent and waxdip is inputted into box body by open-work successively
It is interior, eventually arrive at sample by penetrating into cavernous body；
S3：After waxdip step terminates, the clamping device of the floor installation to the slicer that are dehydrated waxdip box is cut into slices.
Waxdip processing procedure in step s 2, liquid paraffin will contaminate cavernous body and sample, be formed after paraffin cooling
Include the wax stone of sample.
A kind of histopathology sample dehydration waxdip box for exempting from embedding of the present invention, it is advantageous that：
1st, after sample is put into, fixation, washing, dehydration, transparent and waxdip these steps can be carried out continuously without compartment of terrain, it is not necessary to
Moving specimens, while sample is the embedding effect in having reached conventional procedures during waxdip, can be achieved to exempt to embed, can be big
It is big to improve the efficiency made in pathological section；
2nd, base can be connected with the clamping mouth of pathologic section slicer, and can be directly loadable into slicer after completion waxdip is cut into slices；
3rd, the bearing part of sample is cavernous body, has preferable stationarity to sample after contaminating liquid, while cavernous body can resist
Organic solvent in various processes, do not influence chipping qualities while exempting and embedding.
Brief description of the drawings
Fig. 1 is the structural representation of present invention dehydration waxdip box；
Fig. 2 present invention dehydration waxdip boxes are equipped with the design sketch of sample.
Illustrate：1- box bodys, 2- lids, 3- bases, 4- cavernous bodies, 5- open-works, 6- buckles, 7- tangent lines, 8- samples.
Embodiment
With reference to Figure of description, the present invention is described further.
A kind of histopathology sample dehydration waxdip box for exempting from embedding as shown in Figure 1, including box body（1）, lid（2）With
Base（3）, and box body（1）It has been built into and box body（1）The consistent cavernous body of space size（4）, sample（8）It is placed in cavernous body
（4）The gap that is formed of top tangent line in, box body（1）, lid（2）And base（3）It is made of acetal resin.
As depicted in figs. 1 and 2, box body（1）Surface is evenly distributed with open-work（5）, open-work（5）A diameter of 2mm, adjacent open-work
（5）Spacing is 1mm, so as to fixing, washing in use, being dehydrated, transparent and waxdip step corresponding reagent passes through open-work
（5）Penetrate into cavernous body（4）In.Lid（2）Located at box body（1）Upper surface, and and box body（1）The side at edge forms turnover
Connection, the lid（2）Dorsal edge is arranged with buckle（6）, box body（1）Corresponding buttonhole is provided with correspondence position, in box body
（1）Cover it is rear difficult for drop-off, meanwhile, lid（2）Surface is also provided with and above-mentioned box body（1）Identical open-work（5）.Base（3）With
Box body（1）Bottom surface connect and be integrally formed, the base（3）The sample clamping mouth of tissue pathological slice machine can be loaded, make completion
The clamp port that the dehydration waxdip box of variety of processes can be directly loadable into slicer carries out slicing treatment.
As depicted in figs. 1 and 2, cavernous body（4）Top 2/3 at transverse and longitudinal be provided be in latticed tangent line（7）.It is parallel and
Adjacent tangent line（7）Spacing is 3mm, each tangent line（7）Make cavernous body（4）Form the wire gap of transverse and longitudinal, pending sample
Insert tangent line（7）In the gap formed, sample can be strapped in the position being originally placed, meanwhile, used in various processes
Liquid can also pass through cavernous body（4）And act on sample.Cavernous body（4）Can the dissolving of anti-organic solvent, corrosion etc., using process
In, it can effectively avoid the organic solvent that each step uses from ensureing quality tissue slices to sponge precursor reactant.
Using the dehydration waxdip box of the present embodiment, the occupation mode of each step is in pathological section：
1st, draw materials：Representative sample is gathered from the tissue of bulk（8）, or by the tissue of fritter directly as sample
（8）, open the lid for being dehydrated waxdip box（2）, sample is gripped with tweezers（8）, it is positioned over tangent line（7）The superficial part in gap, will be to be cut
Towards lid（2）Direction is placed, and makes sample（8）Treat section and cavernous body（4）Upper surface it is concordant, cover lid（2）.
2nd, fixed, washing, dehydration, transparent and waxdip：According to traditional sample processing steps, it will fix successively, and be dehydrated, be transparent
Pass through open-work with the reagent needed for waxdip（5）Input box body（1）Interior, reagent will reach sample by being dehydrated the sponge of waxdip box
（8）；
3rd, wax stone is formed：At the end of waxdip step, liquid paraffin will be cavernous body（4）And sample（8）Contaminate, by frost or
Natural cooling order dehydration waxdip box temperature is reduced to below melting point of paraffin wax, and formation includes sample（8）Wax stone, directly reach with
" embedding " identical effect in conventional procedures；
4th, cut into slices：The base of waxdip box will be dehydrated（3）The clamping device for being installed to slicer is cut into slices, the first stage of section
Lid（2）It will be cut away by blade, and expose sample after further being cut toward deep（8）And its cavernous body of surrounding（4）, sample（8）With
Cavernous body（4）It is respectively positioned in the paraffin of solidification, using being cut into slices with conventional method identical dicing method and follow-up processing
Step.The difference commonly cut into slices with tradition is the sea suitable with tissue thickness around the section obtained by the above method be present
Silk floss section, these sponges section tissue treatment is not had an impact, and by follow-up dewaxing process voluntarily with histotomy
Separation, directly remove.
It is described above only to express the preferred embodiment of the present invention, its describe it is more specific and in detail, but can not be because
This and be interpreted as the limitation to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art,
On the premise of not departing from present inventive concept, some deformations can also be made, improves and substitutes, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (9)
- A kind of 1. histopathology sample dehydration waxdip box for exempting from embedding, it is characterised in that including：Open-work is distributed with-box body, the outer wall of the box body, for loading and penetrating into corresponding liquid；- lid, located at the cassette upper end, and provided with the clip for being used to be connected with box body, the lid surface distributed has above-mentioned Box body identical open-work；- base, it is integrally formed with the bottom of the box body, for coordinating the clamping mouth of waxdip box and tissue pathological slice machine Connection；- cavernous body, insert in the box body, and consistent with the space size in box body, the cavernous body in top be provided with it is some to The tangent line of lower extension, pending sample are inserted in the gap that tangent line is formed.
- 2. a kind of histopathology sample dehydration waxdip box for exempting from embedding according to claim 1, it is characterised in that described Box body, lid and base are selected from acetal resin.
- 3. a kind of histopathology sample dehydration waxdip box for exempting from embedding according to claim 1, it is characterised in that described Edge corresponding to the edge of lid side and the box body connects to may be reversed.
- A kind of 4. histopathology sample dehydration waxdip box for exempting from embedding according to claim 1 or 2, it is characterised in that The clip is buckle, is symmetrically set in the inner side of the lid, and the box body is provided with corresponding buttonhole in correspondence position.
- 5. a kind of histopathology sample dehydration waxdip box for exempting from embedding according to claim 1, it is characterised in that described Open-work is uniformly distributed in the outer wall of the box body and the lid surface, a diameter of 0.5-3mm of open-work, adjacent open-work spacing For 1-5mm.
- 6. a kind of histopathology sample dehydration waxdip box for exempting from embedding according to claim 1, it is characterised in that described Tangent line is set for transverse and longitudinal, forms the cavernous body upper surface latticed.
- 7. a kind of histopathology sample dehydration waxdip box for exempting from embedding according to claim 6, it is characterised in that parallel And the adjacent tangent line spacing is 1-5mm.
- 8. a kind of histopathology sample dehydration waxdip box for exempting from embedding according to claim any one of 1-7, it is used Method comprises the following steps：S1:Tissue specimen is placed in the gap of tangent line formation on cavernous body top, make tissue specimen treats section and cavernous body Upper surface it is concordant；S2：According to tissue specimen processing step, the reagent needed for fixation, dehydration, transparent and waxdip is inputted by open-work successively In box body, tissue specimen is eventually arrived at by penetrating into cavernous body；S3：After waxdip step terminates, the temperature of dehydration waxdip box is down to paraffin melting point once, form wax stone, waxdip will be dehydrated The clamping device of the floor installation of box to slicer is cut into slices.
- 9. a kind of application method of histopathology sample dehydration waxdip box for exempting from embedding according to claim 8, it is special Sign is：Waxdip processing procedure in step s 2, liquid paraffin will contaminate cavernous body and tissue specimen, and together with box body one And wrap up, form the wax stone for including tissue specimen after paraffin cooling.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710885242.7A CN107576558A (en) | 2017-09-26 | 2017-09-26 | A kind of histopathology sample dehydration waxdip box for exempting from embedding and application method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710885242.7A CN107576558A (en) | 2017-09-26 | 2017-09-26 | A kind of histopathology sample dehydration waxdip box for exempting from embedding and application method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108267329A (en) * | 2018-03-20 | 2018-07-10 | 南昌德漫多科技有限公司 | A kind of sampling device of histopathology sample |
| CN108801749A (en) * | 2018-05-18 | 2018-11-13 | 郑州大学第附属医院 | A kind of method and device making one-pass molding organization chip acceptor wax block |
| CN110376048A (en) * | 2019-08-01 | 2019-10-25 | 邱子晏 | A kind of biopsy specimen embedded box bed die of automatic embedding machine |
| CN110608934A (en) * | 2019-10-19 | 2019-12-24 | 杭州海世嘉生物科技有限公司 | Independent dehydration embedding assembly and use method thereof |
| CN111982647A (en) * | 2020-08-21 | 2020-11-24 | 西南医科大学附属中医医院 | Micro tissue paraffin embedding pretreatment kit and use method thereof |
| CN112161857A (en) * | 2020-10-16 | 2021-01-01 | 胡盛博 | Dehydration embedding box for human tissue specimen examination |
| CN113251751A (en) * | 2021-05-18 | 2021-08-13 | 枣庄科技职业学院 | Pathological section gradient dewatering device |
| WO2023082079A1 (en) * | 2021-11-10 | 2023-05-19 | Leica Biosystems Nussloch Gmbh | Cassette and embedding method using same |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108267329A (en) * | 2018-03-20 | 2018-07-10 | 南昌德漫多科技有限公司 | A kind of sampling device of histopathology sample |
| CN108801749A (en) * | 2018-05-18 | 2018-11-13 | 郑州大学第附属医院 | A kind of method and device making one-pass molding organization chip acceptor wax block |
| CN110376048A (en) * | 2019-08-01 | 2019-10-25 | 邱子晏 | A kind of biopsy specimen embedded box bed die of automatic embedding machine |
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| CN111982647A (en) * | 2020-08-21 | 2020-11-24 | 西南医科大学附属中医医院 | Micro tissue paraffin embedding pretreatment kit and use method thereof |
| CN112161857A (en) * | 2020-10-16 | 2021-01-01 | 胡盛博 | Dehydration embedding box for human tissue specimen examination |
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| WO2023082079A1 (en) * | 2021-11-10 | 2023-05-19 | Leica Biosystems Nussloch Gmbh | Cassette and embedding method using same |
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