JPH0257854B2 - - Google Patents

Description

Detailed Description of the Invention (Technical Field) The present invention relates to an embedding method for making a paraffin-embedded piece by infiltrating a tissue piece with paraffin and surrounding it with paraffin in the process of making a biological tissue piece into a microscopic specimen. .

(Background Art) Observation of tissue pieces collected from the affected areas of experimental animals and patients under a microscope is widely used for research and diagnosis.

To prepare a biological tissue piece into a microscopic specimen, an embedding process is performed in which the tissue piece, which has undergone dehydration, degreasing, etc., is immersed in molten paraffin, the paraffin penetrates into the tissue, and the surrounding area is wrapped in paraffin.

The embedded tissue piece is attached to a slicer together with solidified paraffin, sliced with a microtome, pasted on a slide glass, deparaffinized, stained, and covered with a cover glass to obtain a microscopic specimen.

In the conventional embedding method, for example, as illustrated in FIG. 1, two L-shaped molds 2 are combined on a bottom plate 1 to create a rectangular space, and a piece of biological tissue 3 is placed in this square space. After the paraffin has solidified, the bottom plate 1 and the molds 2 are disassembled to take out the paraffin-embedded piece 4 that has wrapped the tissue piece and solidified into a cubic shape. The material is melted and adhered to a rectangular parallelepiped-shaped rootstock 5 as shown in Figure 2, and then transferred to the slicing process using a microtome knife.

Instead of using the bottom plate 1, forms 2, 2, and rootstock 5, there is also a method of using a small perforated plastic container. In this method, tissue pieces are placed in a stainless steel dish, molten paraffin is injected, a small porous plastic container is placed on top of the dish to solidify the paraffin, and the tissue piece is wrapped in paraffin and embedded on the outside of the small container. It is used to glue the pieces together.

In either case, the rootstock 5 or a small plastic porous container is held between the chucks of the slicer and cut with a microtome knife.
The direction of movement of the tissue piece and the microtome blade becomes constant. Some tissue pieces have a certain direction when sliced, and depending on the direction of movement of the microtome knife, some tissue pieces cannot be sliced well.
It was inconvenient.

(Objective of the present invention) The present invention provides an embedding method capable of producing a paraffin-embedded piece that can arbitrarily adjust its relative position with a microtome knife and that can be easily attached to and detached from a slicer. The purpose is to

(Structure of the present invention) This invention places a piece of biological tissue in a mold box, covers it with a piece of perforated iron plate, injects molten paraffin into the mold box through the hole and solidifies it, and wraps the tissue piece in paraffin. A paraffin-embedded piece is made, and the embedded piece is removed from the mold box while attached to the iron plate piece. This paraffin-embedded piece is attached to a slicer equipped with a magnetic chuck with an iron plate and sliced.

(Embodiment of the present invention) Figs. 3 and 4 illustrate a mold box 6 used for carrying out the present invention. There is an iron plate piece 7 on the side of the mold box 6 (Fig. 5).
A stepped portion 6a is formed for hanging the parts. The stepped portion 6a does not need to be formed on all four sides, and may be formed only on two opposing sides. A hole 8 is bored in the iron plate piece 7 in order to strengthen the bond with the solidified paraffin and to inject molten paraffin. It is also effective to roughen the bonding surface of the iron plate piece in order to strengthen the bond with the paraffin.

FIGS. 6A to 6D are schematic diagrams illustrating the steps of the biological tissue fragment embedding method according to the present invention. Next, the embedding process will be explained with reference to each figure.

FIG. 6A: A small amount of molten paraffin is poured into the mold box 6 to form a thin paraffin layer 9 on the bottom.

FIG. 6B: The biological tissue piece 3 is placed on the paraffin layer 9, and the iron plate piece 7 is placed on the stepped portion 6a.

FIG. 6C: Molten paraffin is injected into the mold box 6 through the hole 8 of the iron plate piece 7, and the mold box 6 is filled.
When the upper surface of the molten paraffin accumulated in the mold box enters the hole 8, the injection is stopped. Due to the initially cast paraffin layer 9, the tissue piece 3 is embedded in the paraffin without being in direct contact with the bottom of the mold box 6.

FIG. 6D: After the paraffin has solidified, the embedded piece with the paraffin-embedded piece 4 stuck to the iron plate piece 7 is taken out. For this removal, if the mold box 6 is placed on a cooling plate and its lower surface is cooled, the embedded piece can be easily taken out from the contact area between the mold box and the paraffin-embedded piece 4.

The paraffin that has built up and solidified from the holes 8 is removed by moving the knife along the iron plate piece.

To slice the paraffin-embedded piece with a microtome, use the magnetic chuck 10 attached to the slicer.
(FIG. 7), the iron plate piece 7 is adsorbed and fixed, and the microtome knife 11 is driven to slice it into thin pieces.

If you want to change the fixed angle to the microtome blade depending on the nature of the biological tissue piece, use the magnetic force chuck 1.
The angle of the embedding piece can be changed freely by operating the handle 10a of 0 to block the magnetic force, and then the handle 10a is operated to attract the iron plate piece 7 again to fix the embedding piece.

(Effects of the present invention) The method for embedding a microscopic specimen of the present invention, which is processed as described above, has the following effects.

(1) When molten paraffin is injected to embed a biological tissue piece, a paraffin-embedded piece is obtained, to which an iron plate piece 7 corresponding to a conventional rootstock is attached at the same time.

There is no need for a separate step of attaching the rootstock 5 to the embedding piece as in the conventional method.

(2) Since it is attached to the slicer by being held by a magnetic chuck, it is easy to attach and detach.

(3) The attachment angle of the paraffin-embedded piece can be adjusted freely, allowing thin sections to be cut at the optimal angle.

(4) Biological tissue pieces are often embedded in paraffin and stored attached to rootstocks, and then taken out and sliced when necessary to make microscopic specimens. Since it is thin, it does not take up as much space when stored as before.

[Brief explanation of the drawing]

FIG. 1 is a perspective view illustrating a conventional embedding method, FIG. 2 is a perspective view showing a paraffin embedding piece attached to a conventional rootstock, and FIG. The figure is a perspective view of the mold box, and Figure 4 is A of Figure 3.
-A sectional view, Figure 5 is a perspective view of the iron plate piece, Figure 6A
-C are longitudinal cross-sectional schematic diagrams showing the embedding process, FIG. 6D is a perspective view, and FIG. 7 is a schematic diagram showing the state in which it is attached to a magnetic chuck and sliced. 1: Bottom plate, 2: Formwork, 3: Biological tissue piece, 4: Paraffin-embedded piece, 5: Rootstock, 6: Mold box, 7: Iron plate piece, 8: Hole, 9: Paraffin layer, 10: Magnetic chuck , 10a: Handle, 11: Microtome sword.

Claims (1)

[Claims] 1. After placing a biological tissue piece in a mold box, the mold box is covered with a piece of perforated iron plate, and then molten paraffin is injected into the mold box body through the hole made in this iron plate piece and solidified. A method for embedding a microscopic specimen in which an embedded piece is made by wrapping the biological tissue piece in paraffin, and the paraffin-embedded piece is removed from a mold box while being adhered to an iron plate piece.