WO2007098951A3 - Method and apparatus for extraction of protein material from silkworm glands - Google Patents

Method and apparatus for extraction of protein material from silkworm glands Download PDF

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Publication number
WO2007098951A3
WO2007098951A3 PCT/EP2007/001775 EP2007001775W WO2007098951A3 WO 2007098951 A3 WO2007098951 A3 WO 2007098951A3 EP 2007001775 W EP2007001775 W EP 2007001775W WO 2007098951 A3 WO2007098951 A3 WO 2007098951A3
Authority
WO
WIPO (PCT)
Prior art keywords
glands
container
extraction
gland
buffer solution
Prior art date
Application number
PCT/EP2007/001775
Other languages
French (fr)
Other versions
WO2007098951A2 (en
Inventor
Michael Rheinnecker
Stefan Kohlhaas
Rolf Zimmat
Original Assignee
Spintech Engineering Gmbh
Michael Rheinnecker
Stefan Kohlhaas
Rolf Zimmat
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spintech Engineering Gmbh, Michael Rheinnecker, Stefan Kohlhaas, Rolf Zimmat filed Critical Spintech Engineering Gmbh
Priority to DK07703571.5T priority Critical patent/DK1989223T3/en
Priority to JP2008556718A priority patent/JP5269616B2/en
Priority to CA2642793A priority patent/CA2642793C/en
Priority to EP07703571A priority patent/EP1989223B1/en
Priority to AT07703571T priority patent/ATE458001T1/en
Priority to DE602007004830T priority patent/DE602007004830D1/en
Priority to PL07703571T priority patent/PL1989223T3/en
Publication of WO2007098951A2 publication Critical patent/WO2007098951A2/en
Publication of WO2007098951A3 publication Critical patent/WO2007098951A3/en
Priority to US12/128,425 priority patent/US8287913B2/en
Priority to US12/486,977 priority patent/US8105633B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
    • C12M3/062—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means with flat plate filter elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/08—Apparatus for tissue disaggregation
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01B—MECHANICAL TREATMENT OF NATURAL FIBROUS OR FILAMENTARY MATERIAL TO OBTAIN FIBRES OF FILAMENTS, e.g. FOR SPINNING
    • D01B7/00—Obtaining silk fibres or filaments

Abstract

An apparatus and method for the extraction of material from glands of arthropods. The apparatus (5) comprises a container (10) in which at least part of the glands are placed and a buffer solution (30) at least partially immersing the glands. Gland material is collected in the material collection area (20) of container (10). In use the material is released from glands into the buffer solution (30) and sedimented at the bottom of the container (10). The method comprises: a first step of removing from a body of the arthropod the gland containing at least partially the material; a second step of making an opening in an epithelium of the gland; and a third step of placing the gland in a container (10) at least partially immersed in a buffer solution (30) such that the materials exit the glands and sediments in the material collection area (20) of container (10).
PCT/EP2007/001775 2006-03-01 2007-03-01 Method and apparatus for extraction of protein material from silkworm glands WO2007098951A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DK07703571.5T DK1989223T3 (en) 2006-03-01 2007-03-01 Method and apparatus for extracting glands from arthropods
JP2008556718A JP5269616B2 (en) 2006-03-01 2007-03-01 Arthropod gland extraction method and apparatus
CA2642793A CA2642793C (en) 2006-03-01 2007-03-01 Method and apparatus for extraction of protein material from silkworm glands
EP07703571A EP1989223B1 (en) 2006-03-01 2007-03-01 Method and apparatus for extraction of arthropod gland
AT07703571T ATE458001T1 (en) 2006-03-01 2007-03-01 METHOD AND DEVICE FOR EXTRACTING AN ARTHROPOD GLAND
DE602007004830T DE602007004830D1 (en) 2006-03-01 2007-03-01 METHOD AND DEVICE FOR EXTRACTION OF AN ARTHROPOD SPRAY
PL07703571T PL1989223T3 (en) 2006-03-01 2007-03-01 Method and apparatus for extraction of arthropod gland
US12/128,425 US8287913B2 (en) 2006-03-01 2008-05-28 Method and apparatus for extraction of protein material from arthropod glands and a membrane formed therefrom
US12/486,977 US8105633B2 (en) 2006-03-01 2009-06-18 Method and apparatus for extraction of arthropod gland

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US77778806P 2006-03-01 2006-03-01
US60/777,788 2006-03-01
GB0604089.3 2006-03-01
GB0604089A GB2435646A (en) 2006-03-01 2006-03-01 Apparatus and method of extraction of an arthropod gland

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/128,425 Continuation-In-Part US8287913B2 (en) 2006-03-01 2008-05-28 Method and apparatus for extraction of protein material from arthropod glands and a membrane formed therefrom

Publications (2)

Publication Number Publication Date
WO2007098951A2 WO2007098951A2 (en) 2007-09-07
WO2007098951A3 true WO2007098951A3 (en) 2007-10-18

Family

ID=36218912

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/001775 WO2007098951A2 (en) 2006-03-01 2007-03-01 Method and apparatus for extraction of protein material from silkworm glands

Country Status (12)

Country Link
US (1) US8287913B2 (en)
EP (1) EP1989223B1 (en)
JP (1) JP5269616B2 (en)
AT (1) ATE458001T1 (en)
CA (1) CA2642793C (en)
DE (1) DE602007004830D1 (en)
DK (1) DK1989223T3 (en)
ES (1) ES2339497T3 (en)
GB (1) GB2435646A (en)
PL (1) PL1989223T3 (en)
PT (1) PT1989223E (en)
WO (1) WO2007098951A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8105633B2 (en) * 2006-03-01 2012-01-31 Spintec Engineering Gmbh Method and apparatus for extraction of arthropod gland
WO2010042798A2 (en) 2008-10-09 2010-04-15 Trustees Of Tufts College Modified silk films containing glycerol
GB2464348A (en) 2008-10-17 2010-04-21 Spintec Engineering Gmbh Applying a liquid protein onto a permeable surface, and silk mono-filament having specific properties
EP2253336A1 (en) 2009-05-15 2010-11-24 Spintec Engineering GmbH A silk medical device with antimicrobial properties and a method of manufacture thereof
US10335519B2 (en) 2011-04-20 2019-07-02 Trustees Of Tufts College Dynamic silk coatings for implantable devices
CA2869967A1 (en) 2012-04-13 2013-10-17 Trustees Of Tufts College Methods and compositions for preparing a silk microsphere
EP2841108A4 (en) 2012-04-25 2015-11-25 Tufts College Silk microspheres and methods for surface lubrication
WO2014012101A1 (en) 2012-07-13 2014-01-16 Trustees Of Tufts College Silk powder compaction for production of constructs with high mechanical strength and stiffness
EP2719374A1 (en) 2012-10-12 2014-04-16 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen Drug delivery device
US11376329B2 (en) 2013-03-15 2022-07-05 Trustees Of Tufts College Low molecular weight silk compositions and stabilizing silk compositions
JP6457482B2 (en) 2013-03-15 2019-01-23 トラスティーズ オブ タフツ カレッジ Low molecular weight silk composition and stabilization of silk composition
US10285702B2 (en) 2013-04-24 2019-05-14 Trustees Of Tufts College Bioresorbable biopolymer anastomosis devices
GB201501134D0 (en) * 2015-01-23 2015-03-11 Vollrath Friedrich W L Raw material for bio-spinning casting
CN104894655B (en) * 2015-06-30 2017-06-16 广西立盛茧丝绸有限公司 A kind of cocoons-boiling method of fresh cocoon
WO2017192227A1 (en) 2016-05-04 2017-11-09 Trustees Of Tufts College Silk nanofibrils and uses thereof
CN108774750A (en) * 2018-05-31 2018-11-09 杭州金利丝业有限公司 A kind of new type water silk floss machine
WO2020028918A1 (en) * 2018-08-03 2020-02-06 Trustees Of Tufts College Automated process for silk fibroin extraction

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2003037925A2 (en) * 2001-10-31 2003-05-08 Fritz Vollrath Precursor silk feedstock for forming filaments
JP2003165843A (en) * 2001-11-29 2003-06-10 Okamoto Lace Kk Method for extracting sericin, the extract solution and method for modifying fiber or fabric

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JPS5167785A (en) 1974-12-02 1976-06-11 Mihama Kk Fuyokakosono seizohoho
JPH02268693A (en) * 1989-04-10 1990-11-02 Asahi Chem Ind Co Ltd Production of silk fibroin by culturing rear silk glands
JPH03209399A (en) * 1990-01-11 1991-09-12 Terumo Corp Production of fibroin
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Publication number Priority date Publication date Assignee Title
WO2003037925A2 (en) * 2001-10-31 2003-05-08 Fritz Vollrath Precursor silk feedstock for forming filaments
JP2003165843A (en) * 2001-11-29 2003-06-10 Okamoto Lace Kk Method for extracting sericin, the extract solution and method for modifying fiber or fabric

Non-Patent Citations (1)

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Also Published As

Publication number Publication date
ATE458001T1 (en) 2010-03-15
EP1989223B1 (en) 2010-02-17
EP1989223A2 (en) 2008-11-12
US8287913B2 (en) 2012-10-16
DK1989223T3 (en) 2010-06-07
CA2642793C (en) 2013-10-01
GB0604089D0 (en) 2006-04-12
ES2339497T3 (en) 2010-05-20
JP5269616B2 (en) 2013-08-21
PL1989223T3 (en) 2010-07-30
US20080293918A1 (en) 2008-11-27
PT1989223E (en) 2010-04-14
WO2007098951A2 (en) 2007-09-07
GB2435646A (en) 2007-09-05
CA2642793A1 (en) 2007-09-07
JP2009528314A (en) 2009-08-06
DE602007004830D1 (en) 2010-04-01

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