CN111982647A - Micro tissue paraffin embedding pretreatment kit and use method thereof - Google Patents
Micro tissue paraffin embedding pretreatment kit and use method thereof Download PDFInfo
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- CN111982647A CN111982647A CN202010846919.8A CN202010846919A CN111982647A CN 111982647 A CN111982647 A CN 111982647A CN 202010846919 A CN202010846919 A CN 202010846919A CN 111982647 A CN111982647 A CN 111982647A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/366—Moulds; Demoulding
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of kits, in particular to a paraffin embedding pretreatment kit for micro tissues, which comprises a reagent box body, a cover plate and an operation pore plate, wherein the operation pore plate is provided with a reaction hole, a filter groove, a forceps groove, a water absorption paper using groove and a water absorption paper storage groove, and the reaction hole sequentially comprises a 40% polymethyl alcohol hole, a 70% alcohol hole, an 85% alcohol hole, a 95% alcohol hole, a 100% alcohol hole, a xylene hole and a liquid paraffin hole in an anticlockwise arrangement mode. The invention also provides a use method of the micro-tissue paraffin embedding pretreatment kit, the kit comprises various prepared soaking solutions, and the liquid preparation time and the reagent loss are saved; the method has the advantages of simple operation, few experimental steps, short time, good protection of the shape of the micro tissue, no influence on the concentration of the soak solution, more reliable data, market prospect and suitability for popularization.
Description
Technical Field
The invention relates to the technical field of kits, in particular to a paraffin embedding pretreatment kit for a micro tissue and a using method thereof.
Background
The immunostaining technique is the most commonly used technique for studying tissue/cell antigens and can perform qualitative, positioning and quantitative determination on the antigens. Before immunostaining, tissues/cells must be fixed, dehydrated, cleared, paraffin embedded, sectioned, etc. For the experimental steps of fixing, dehydrating, transparentizing and the like of micro tissues (such as blastula, stem cell balls and the like, the volume is only 0.2-1.0 mm), the soaking of the micro tissues in different liquids is realized by repeatedly transferring liquid by a liquid transferring gun and centrifuging and removing the liquid by a centrifugal machine in the traditional method. The traditional operation method has the following disadvantages: 1. the immersion liquid of the micro tissue can not be completely removed by centrifugation and liquid transfer, so that the concentration deviation of the liquid is caused, and the fixing, dehydrating and transparent effects are poor; 2. repeated pipetting operations can lead to a reduction in the overall number by taking away parts of the micro-tissue; 3. repeated pipetting operations can cause minute tissue deformations; 4. the preparation of alcohols of different concentrations and the numerous operating steps are time-consuming and laborious. Therefore, a micro tissue paraffin embedding pretreatment kit and a use method thereof are provided to solve the problems.
Disclosure of Invention
The invention aims to solve the defects of poor effect and large experimental error of the existing experimental steps of fixing, dehydrating, transparentizing and the like of the micro tissue in the prior art, and provides a micro tissue paraffin embedding pretreatment kit and a using method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a small tissue paraffin embedding pretreatment kit, includes reagent box body, apron and operation orifice plate, be equipped with reaction hole, filter groove, tweezers groove, absorbent paper use groove and absorbent paper storage tank on the operation orifice plate, the reaction hole is arranged according to anticlockwise and is included 40% polymer methyl alcohol hole, 70% alcohol hole, 85% alcohol hole, 95% alcohol hole, 100% alcohol hole, xylene hole and liquid paraffin hole in proper order.
Preferably, the number of the 95% alcohol holes, the 100% alcohol holes and the xylene holes is two.
Preferably, the number of the filter grooves is four.
Preferably, the bottom of the reagent box body is provided with a storage drawer plate through a sliding chute structure in a drawing and pulling manner, and the storage drawer plate is provided with a 40% poly-methanol reagent bottle, a xylene reagent bottle, a 70% alcohol reagent bottle, an 85% alcohol reagent bottle, a 95% alcohol reagent bottle, a 100% alcohol reagent bottle, absorbent paper and a filter.
Preferably, the filter is a metal structure, and the pore size of the filter pores on the filter is 100 meshes.
A use method of a microscopic tissue paraffin embedding pretreatment kit comprises the following steps:
s1, taking various reagent bottles from the storage drawer plate, sequentially guiding the reagent bottles into corresponding reaction holes in the operation hole plate, taking a filter from the storage drawer plate and putting the filter into a filter tank, and taking absorbent paper from the storage drawer plate and putting the absorbent paper into an absorbent paper storage tank;
s2, placing the micro tissue into a filter, and clamping the filter by using forceps stored in a forceps tank to perform enzyme component destruction operation through 40% of a poly-methanol hole;
s3, using tweezers to sequentially pass the filter subjected to the enzyme component destruction operation of the step S2 through a 70% alcohol hole, an 85% alcohol hole, a 95% alcohol hole and a 100% alcohol hole to perform gradation dehydration operation;
and S4, performing alcohol replacement on the filter xylene-passing holes subjected to the step S3-degree dehydration operation by using forceps, performing xylene replacement on the filter xylene-passing holes, and cooling the filter after the replacement, thereby completing the pretreatment operation of the micro-tissue paraffin embedding.
Preferably, the number of operations of passing through the 95% alcohol well, the 100% alcohol well and the xylene well is two in steps S3 and S4.
Preferably, every time the filter is transferred in the reaction well, the liquid around the filter is adsorbed clean by using absorbent paper.
The invention provides a paraffin embedding pretreatment kit for micro tissues and a using method thereof, and the kit has the beneficial effects that: the kit disclosed by the invention contains various prepared soaking solutions, so that the liquid preparation time is greatly saved, and the reagent loss of liquid preparation is greatly reduced; according to the invention, a liquid transfer gun is not required to be used for transferring liquid, so that the loss of the micro tissue is reduced, and the possibility of deformation of the micro tissue is reduced; the invention does not need repeated centrifuge centrifugation, thereby reducing the experimental cost; meanwhile, the method is simple to operate, few in experimental steps, short in time, good in protection of the shape of the micro tissue, free of influence on the concentration of the soaking solution, reliable in data, and suitable for popularization, and has market prospects.
Drawings
FIG. 1 is a schematic structural diagram of a pretreatment kit for paraffin embedding of a micro tissue according to the present invention;
FIG. 2 is a schematic diagram of an explosion structure of a pretreatment kit for paraffin embedding of a micro tissue according to the present invention;
fig. 3 is a schematic structural view of a drawer receiving plate according to the present invention;
fig. 4 is a schematic structural view of an operation orifice plate proposed in the present invention.
In the figure: the reagent cartridge 1 includes a drawer plate 2, a 40% methanol reagent bottle 21, a xylene reagent bottle 22, a 70% alcohol reagent bottle 23, an 85% alcohol reagent bottle 24, a 95% alcohol reagent bottle 25, a 100% alcohol reagent bottle 26, absorbent paper 27, a filter 28, a cover plate 3, an operation orifice plate 4, a 40% methanol reagent hole 41, a 70% alcohol hole 42, an 85% alcohol hole 43, a 95% alcohol hole 44, a 100% alcohol hole 45, a xylene hole 46, a liquid paraffin hole 47, a filter tank 48, a forceps tank 49, an absorbent paper use tank 401, and an absorbent paper storage tank 402.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-4, a pretreatment kit for paraffin embedding of a micro tissue, comprising a reagent kit body 1, a cover plate 3 and an operation orifice plate 4, wherein the operation orifice plate 4 is provided with reaction holes, a filter groove 48, a forceps groove 49, a water absorption paper using groove 401 and a water absorption paper storing groove 402, the reaction holes are arranged anticlockwise and sequentially comprise 40% of poly-methanol holes 41, 70% of alcohol holes 42, 85% of alcohol holes 43, 95% of alcohol holes 44, 100% of alcohol holes 45, xylene holes 46 and liquid paraffin holes 47, wherein the number of the 95% of alcohol holes 44, the 100% of alcohol holes 45 and the xylene holes 46 is two, and the number of the filter grooves 48 is four.
The bottom of reagent box body 1 is equipped with through chute structure pull formula connection and accomodates drawer plate 2, be convenient for wholly accomodate, the convenience of use has been promoted, also repeatedly usable simultaneously, it is equipped with 40% polymer methyl alcohol reagent bottle 21 on drawer plate 2 to accomodate, xylol reagent bottle 22, 70% alcohol reagent bottle 23, 85% alcohol reagent bottle 24, 95% alcohol reagent bottle 25, 100% alcohol reagent bottle 26, absorbent paper 27 and filter 28, filter 28 is metal structure, the aperture of the filtration pore on the filter 28 is 100 meshes.
The use method of the microscopic tissue paraffin embedding pretreatment kit is characterized by comprising the following steps:
s1, taking various reagent bottles from the storage drawer plate 2, sequentially guiding the reagent bottles into corresponding reaction holes in the operation hole plate 4, taking the filter 28 from the storage drawer plate 2 and putting the filter 28 into the filter groove 48, and taking the absorbent paper 27 from the storage drawer plate 2 and putting the absorbent paper into the absorbent paper storage groove 402;
s2, placing the micro-tissue into the filter 28, and grasping the filter 28 with forceps stored in the forceps tank 49 to perform enzyme component destruction operation through the 40% polymethyl alcohol hole 41;
s3, using tweezers to make the filter 28 subjected to the enzyme component destruction operation of the step S2 pass through a 70% alcohol hole 42, an 85% alcohol hole 43, a 95% alcohol hole 44 and a 100% alcohol hole 45 in sequence, and performing a gradation dehydration operation, wherein the number of times of passing through the 95% alcohol hole 44 and the 100% alcohol hole 45 is two;
and S4, performing alcohol replacement operation on the xylene holes 46 of the filter 28 subjected to the step S3 degree dehydration operation by using tweezers, performing xylene replacement operation on the liquid paraffin holes 47 of the filter 28, and cooling the filter 28 after replacement to finish the micro tissue paraffin embedding pretreatment operation, wherein the operation times of the xylene holes 46 are two times, and the liquid around the filter 28 needs to be adsorbed and cleaned by using the absorbent paper 27 every time the filter 28 is transferred in the reaction holes.
The kit disclosed by the invention contains various prepared soaking solutions, so that the liquid preparation time is greatly saved, and the reagent loss of liquid preparation is greatly reduced; according to the invention, a liquid transfer gun is not required to be used for transferring liquid, so that the loss of the micro tissue is reduced, and the possibility of deformation of the micro tissue is reduced; the invention does not need repeated centrifuge centrifugation, thereby reducing the experimental cost; meanwhile, the method is simple to operate, few in experimental steps, short in time, good in protection of the shape of the micro tissue, free of influence on the concentration of the soaking solution, reliable in data, and suitable for popularization, and has market prospects.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. The utility model provides a little tissue paraffin embedding pretreatment kit, includes reagent box body (1), apron (3) and operation orifice plate (4), its characterized in that, be equipped with reaction hole, filter groove (48), tweezers groove (49), absorbent paper on operation orifice plate (4) and use groove (401) and absorbent paper storing tank (402), the reaction hole includes 40% polymer methyl alcohol hole (41), 70% alcohol hole (42), 85% alcohol hole (43), 95% alcohol hole (44), 100% alcohol hole (45), xylene hole (46) and liquid paraffin hole (47) according to anticlockwise arranging in proper order.
2. The pretreatment kit for paraffin embedding of a microscopic tissue according to claim 1, wherein the number of the 95% alcohol wells (44), the 100% alcohol wells (45) and the xylene wells (46) is two.
3. The pretreatment kit for paraffin embedding of a minute tissue as claimed in claim 1, wherein the number of the filter grooves (48) is four.
4. The microscopic tissue paraffin embedding pretreatment kit according to claim 1, wherein a storage drawer plate (2) is arranged at the bottom of the reagent kit body (1) in a drawing type connection through a chute structure, and a 40% polymeric methanol reagent bottle (21), a xylene reagent bottle (22), a 70% alcohol reagent bottle (23), an 85% alcohol reagent bottle (24), a 95% alcohol reagent bottle (25), a 100% alcohol reagent bottle (26), absorbent paper (27) and a filter (28) are arranged on the storage drawer plate (2).
5. The pretreatment kit for paraffin embedding of a microscopic tissue according to claim 4, wherein the filter (28) is of a metal structure, and the pore size of the filter pores on the filter (28) is 100 meshes.
6. The use method of the microscopic tissue paraffin embedding pretreatment kit according to claims 1-5, characterized by comprising the following steps:
s1, taking various reagent bottles from the storage drawer plate (2), sequentially guiding the reagent bottles into corresponding reaction holes in the operation hole plate (4), taking the filter (28) from the storage drawer plate (2) and putting the filter into the filter groove (48), and taking the absorbent paper (27) from the storage drawer plate (2) and putting the absorbent paper into the absorbent paper storage groove (402);
s2, placing the micro-tissue into a filter (28), and clamping the filter (28) by using tweezers stored in a tweezers groove (49) to perform enzyme component destruction operation through a 40% polymethyl alcohol hole (41);
s3, using tweezers to make the filter (28) which is subjected to the enzyme component destruction operation of the step S2 pass through 70% alcohol hole (42), 85% alcohol hole (43), 95% alcohol hole (44) and 100% alcohol hole (45) in sequence, and then carry out the gradation dehydration operation;
and S4, performing alcohol replacement operation on the filter (28) subjected to the dehydration operation of the step S3 degree through a xylene hole (46) by using tweezers, performing xylene replacement operation on the filter (28) through a liquid paraffin hole (47), and cooling the filter (28) after the replacement operation, thereby completing the pretreatment operation of the paraffin embedding of the micro-tissue.
7. The method of claim 1, wherein the 95% alcohol wells (44), the 100% alcohol wells (45) and the xylene wells (46) are performed twice in steps S3 and S4.
8. The method for using the pretreatment kit for paraffin embedding of minute tissue according to claim 1, wherein the liquid around the filter (28) is adsorbed clean by using the water-absorbent paper (27) every time the filter (28) is transferred into the reaction well.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010846919.8A CN111982647A (en) | 2020-08-21 | 2020-08-21 | Micro tissue paraffin embedding pretreatment kit and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010846919.8A CN111982647A (en) | 2020-08-21 | 2020-08-21 | Micro tissue paraffin embedding pretreatment kit and use method thereof |
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| Publication Number | Publication Date |
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| CN111982647A true CN111982647A (en) | 2020-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010846919.8A Pending CN111982647A (en) | 2020-08-21 | 2020-08-21 | Micro tissue paraffin embedding pretreatment kit and use method thereof |
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Citations (13)
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|---|---|---|---|---|
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2020
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