CN207091415U - With the stem cell extract detection kit for promoting senile cell remobilization - Google Patents
With the stem cell extract detection kit for promoting senile cell remobilization Download PDFInfo
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- CN207091415U CN207091415U CN201720748693.1U CN201720748693U CN207091415U CN 207091415 U CN207091415 U CN 207091415U CN 201720748693 U CN201720748693 U CN 201720748693U CN 207091415 U CN207091415 U CN 207091415U
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- China
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- box body
- stem cell
- detection kit
- refrigeration machine
- remobilization
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Abstract
The utility model discloses with the stem cell extract detection kit for promoting senile cell remobilization, including box body, bottom plate, refrigeration machine, battery and sleeve pipe, the box body upper left corner is provided with connecting shaft, and box body is connected by connecting shaft with lid, the refrigeration machine is fixed on below bottom plate, and refrigerator pipe is connected with refrigeration machine, the battery is arranged on the right side of refrigeration machine, and it is provided with charge port on the box body below battery, instrument placing box is provided with the right side of the battery, described sleeve pipe is embedded in inside standing groove, and inside pipe casing is provided with spring, the spring end is connected with fixture block.This, which has, promotes the stem cell extract detection kit of senile cell remobilization to be mainly used for depositing reagent bottle and designing, refrigeration machine is provided with kit can allow this device to continue to place reagent bottle in no refrigerator or in the case of having a power failure, and reduce the limitation of kit storage.
Description
Technical field
Kit technical field is the utility model is related to, specially there is the stem cell for promoting senile cell remobilization
Extract detection kit.
Background technology
With the economic fast development with science and technology, the development of medical science is also very rapid, and stem cell has cell content
The advantages that height, multiplication capacity are strong, and immunogenicity is low, and has convenient material drawing, and no ethics is disputed on, therefore medical field is to dry thin
Born of the same parents carry out more research, and the reagent of extraction can be used when from stem cell extraction of substance, and these reagents need to use specifically
Kit is held, therefore occurs substantial amounts of stem cell extract detection kit on the market, but these kits can only be put into
Refrigerated in refrigerator, the limitation of storage is larger, and the reagent bottle position being placed in kit easily changes, can not
Meets the needs of in real work, therefore there is an urgent need to can improve to promote the stem cell of senile cell remobilization to carry on the market
The technology of analyte detection reagent cartridge configuration is taken, to improve this equipment.
Utility model content
The purpose of this utility model is to provide a kind of stem cell extract for having and promoting senile cell remobilization
Detection kit, to solve the existing stem cell with promotion senile cell remobilization proposed in above-mentioned background technology
The limitation of extract detection kit storage is larger, and what the reagent bottle position being placed in kit easily changed asks
Topic.
To achieve the above object, the utility model provides following technical scheme：With promotion senile cell remobilization
Stem cell extract detection kit, including box body, bottom plate, refrigeration machine, battery and sleeve pipe, the box body upper left corner is provided with
Connecting shaft, and box body is connected by connecting shaft with lid, the lid is internally provided with disinfectant holder, and on the right side of lid
Surface is provided with lock, and the bottom plate is fixed on tray interior, and reagent bottle holding chamber is provided with bottom plate, and the reagent bottle is put
Put chamber interior and be provided with standing groove, the refrigeration machine is fixed on below bottom plate, and refrigerator pipe is connected with refrigeration machine, the battery
It is arranged on the right side of refrigeration machine, and charge port is provided with the box body below battery, instrument placement is provided with the right side of the battery
Case, described sleeve pipe are embedded in inside standing groove, and inside pipe casing is provided with spring, and the spring end is connected with fixture block.
Preferably, the part that the lid overlaps with disinfectant holder is network structure, and disinfectant holder and box
It is flexible connection between lid.
Preferably, the standing groove is equidistant is arranged in reagent bottle holding chamber, and the body wall of standing groove is hollow out knot
Structure.
Preferably, the refrigerator pipe is shaped as S-shaped, and refrigerator pipe is embedded in inside the body wall of box body, and refrigerator pipe
Gas outlet is uniformly provided with box body body wall.
Preferably, described sleeve pipe, spring and fixture block form fixing device, and fixing device is provided with altogether four, and
Fixing device angularly disposes.
Preferably, the fixture block is fastened in the body wall of standing groove, and the surface of fixture block is arc.
Compared with prior art, the beneficial effects of the utility model are：This, which has, promotes senile cell remobilization
Stem cell extract detection kit is mainly used for depositing reagent bottle and designing, can there is provided refrigeration machine in kit
Allow this device to continue to place reagent bottle in no refrigerator or in the case of having a power failure, reduce the limitation of kit storage,
Sleeve pipe, spring and fixture block form fixing device, and fixing device is provided with four altogether, and fixing device angularly disposes,
Fixture block is fastened in the body wall of standing groove, and the surface of fixture block is arc, and preferably reagent bottle can be fixed in standing groove,
The position for avoiding reagent bottle changes, and overall action effect is fine, easy to operation.
Brief description of the drawings
Fig. 1 is the utility model structure diagram；
Fig. 2 is the utility model refrigerator pipe structural representation；
Fig. 3 is that the utility model places slot structure schematic diagram.
In figure：1st, box body, 2, connecting shaft, 3, lid, 4, disinfectant holder, 5, lock, 6, bottom plate, 7, reagent bottle places
Room, 8, standing groove, 9, refrigeration machine, 10, refrigerator pipe, 11, gas outlet, 12, battery, 13, charge port, 14, instrument placing box, 15,
Sleeve pipe, 16, spring, 17, fixture block.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1-3 are referred to, the utility model provides a kind of technical scheme：With the dry of promotion senile cell remobilization
Cell extract detection kit, including box body 1, connecting shaft 2, lid 3, disinfectant holder 4, lock 5, bottom plate 6, reagent bottle
Holding chamber 7, standing groove 8, refrigeration machine 9, refrigerator pipe 10, gas outlet 11, battery 12, charge port 13, instrument placing box 14, sleeve pipe
15th, spring 16 and fixture block 17, the upper left corner of box body 1 is provided with connecting shaft 2, and box body 1 is connected by connecting shaft 2 with lid 3, box
Lid 3 is internally provided with disinfectant holder 4, and the right lateral surface of lid 3 is provided with lock 5, lid 3 and the weight of disinfectant holder 4
The part of conjunction is network structure, and kit inside can be carried out to be flexibly connected between disinfectant holder 4 and lid 3
Sterilization, bottom plate 6 are fixed on inside box body 1, and reagent bottle holding chamber 7 is provided with bottom plate 6, inside reagent bottle holding chamber 7
Standing groove 8 is provided with, standing groove 8 is equidistant to be arranged in reagent bottle holding chamber 7, and the body wall of standing groove 8 is engraved structure,
Cold air caused by refrigeration machine 9 can be made preferably to be circulated in reagent bottle holding chamber 7, refrigeration machine 9 is fixed on the lower section of bottom plate 6, and
Refrigerator pipe 10 is connected with refrigeration machine 9, refrigerator pipe 10 is shaped as S-shaped, and refrigerator pipe 10 is embedded in inside the body wall of box body 1,
And gas outlet 11 is uniformly provided with refrigerator pipe 10 and the body wall of box body 1, reaches faster the temperature in kit
The temperature specified, gas outlet 11 is provided with the body wall of box body 1, in order to avoid the air pressure increase inside kit, battery 12 is set
Put on the right side of refrigeration machine 9, and charge port 13 is provided with the box body 1 of the lower section of battery 12, the right side of battery 12 is provided with instrument and put
Case 14 is put, sleeve pipe 15 is embedded in inside standing groove 8, and sleeve pipe 15 is internally provided with spring 16, sleeve pipe 15, spring 16 and fixture block 17
Fixing device is formed, and fixing device is provided with four altogether, and fixing device angularly disposes, and the end of spring 16 is connected with
Fixture block 17, fixture block 17 are fastened in the body wall of standing groove 8, and the surface of fixture block 17 is arc, and preferably reagent bottle can be entered
Row is fixed, and the position for avoiding reagent bottle changes.
Operation principle：Using this have promote senile cell remobilization stem cell extract detection kit it
Before, it is necessary first to whole there is the stem cell extract detection kit for promoting senile cell remobilization to carry out in structure
Simple knowledge, staff is charged by charge port 13 to battery 12 first, then solves opening lock catch 5, opens the lid 3,
Disinfectant is put into disinfectant holder 4, reagent bottle is put into standing groove 8 afterwards, the pressure of fixture block 17 is made in reagent bottle
Deform upon spring 16 under, and then drive fixture block 17 to shrink, so that reagent bottle is preferably fixed in standing groove 8, so
Close lid 3 afterwards, lock lock 5, start refrigeration machine 9, in the presence of refrigeration machine 9 caused cold air by refrigerator pipe 10 from going out
It is discharged in gas port 11 inside box body 1, gas outlet 11 is provided with the body wall of box body 1, in order to avoid the air pressure inside kit increases
Add, operated when needing to use the reagent bottle in kit, it is necessary to take out supporting instrument from instrument placing box 14, this is just
It is this operation principle with the stem cell extract detection kit for promoting senile cell remobilization.
Experimental method：
The culture of the stem cell of senile cell remobilization：Bone marrow suspension is collected in 15 mL centrifuge tubes, at room temperature
5 min are centrifuged, centrifuge radius 8cm, the r/min of rotating speed 1000；Supernatant is removed after centrifugation, fresh with 5 mL containing volume fraction is 10% tire
α-DMEM the complete culture solutions of cow's serum are resuspended, and gently blow and beat, single cell suspension is made, be transferred in 10 cm culture dishes, be placed in
37 DEG C, volume fraction be 5% CO2 saturated humidity incubators in cultivate.Liquid is changed after about 48 h first, cell reaches after 7-10 d
Fusion, is passed on, is now designated as P1 generations, changes liquid 1 time per couple of days later, is counted every time during passage, calculates cell multiplication generation
Count, cellular morphology and growing state are observed under inverted microscope.
The identification of stem cell：It is placed in 10 mL centrifuge tubes and uses after the mescenchymal stem cell of original cuiture is digested with pancreatin
PBS is rinsed 1 time；5 min, the r/min of rotating speed 1 000 are centrifuged at room temperature, are then inhaled and are abandoned PBS, add 2% bovine serum albumin of precooling
(BSA) 2 mL in vain, quick to mix, 4 DEG C of 30 min of closing；The antiantibody added in kit, it is 1 to make its additional proportion:
10,4 DEG C of 30 min of incubation；5 min, the r/min of rotating speed 1 000 are centrifuged at room temperature, are then inhaled and are abandoned supernatant；2 are washed with PBS
It is resuspended after secondary with PBS, adds secondary antibody (mouse IgG-FITC), its additional proportion is incubated 30 min for 1: 100,4 DEG C of lucifuges.With
PBS is resuspended after washing 2 times with 400 μ L PBS, flow cytomery.
Senescence associated-β-galactosidase detects cell ageing situation：Cell is seeded to 24 orifice plates of preset cover glass,
Cell climbing sheet PBS rinsings 2 times are taken out after cultivating 24 h, fix 15 min, then PBS is rinsed 2 times again, is formulated to specifications
Reaction solution is configured, cell is acted on, 37 DEG C of overnight incubations, optical microphotograph Microscopic observation cell dyeing situation, counts 200 thin
Born of the same parents calculate staining positive cells percentage.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (6)
1. with the stem cell extract detection kit for promoting senile cell remobilization, including box body（1）, bottom plate
（6）, refrigeration machine（9）, battery（12）And sleeve pipe（15）, it is characterised in that：The box body（1）The upper left corner is provided with connecting shaft（2）,
And box body（1）Pass through connecting shaft（2）With lid（3）It is connected, the lid（3）It is internally provided with disinfectant holder（4）, and
Lid（3）Right lateral surface is provided with lock（5）, the bottom plate（6）It is fixed on box body（1）Inside, and bottom plate（6）On be provided with examination
Agent bottle holding chamber（7）, the reagent bottle holding chamber（7）It is internally provided with standing groove（8）, the refrigeration machine（9）It is fixed on bottom plate
（6）Lower section, and refrigeration machine（9）On be connected with refrigerator pipe（10）, the battery（12）It is arranged on refrigeration machine（9）Right side, and battery
（12）The box body of lower section（1）On be provided with charge port（13）, the battery（12）Right side be provided with instrument placing box（14）, institute
State sleeve pipe（15）It is embedded in standing groove（8）Inside, and sleeve pipe（15）It is internally provided with spring（16）, the spring（16）End connects
It is connected to fixture block（17）.
2. according to claim 1 have the stem cell extract detection kit for promoting senile cell remobilization,
It is characterized in that：The lid（3）With disinfectant holder（4）The part of coincidence is network structure, and disinfectant holder（4）
With lid（3）Between for be flexibly connected.
3. according to claim 1 have the stem cell extract detection kit for promoting senile cell remobilization,
It is characterized in that：The standing groove（8）It is equidistant to be arranged on reagent bottle holding chamber（7）It is interior, and standing groove（8）Body wall to engrave
Hollow structure.
4. according to claim 1 have the stem cell extract detection kit for promoting senile cell remobilization,
It is characterized in that：The refrigerator pipe（10）Be shaped as S-shaped, and refrigerator pipe（10）It is embedded in box body（1）Body wall inside, and
Refrigerator pipe（10）And box body（1）Gas outlet is uniformly provided with body wall（11）.
5. according to claim 1 have the stem cell extract detection kit for promoting senile cell remobilization,
It is characterized in that：Described sleeve pipe（15）, spring（16）With fixture block（17）Fixing device is formed, and fixing device is provided with four altogether
It is individual, and fixing device angularly disposes.
6. according to claim 1 have the stem cell extract detection kit for promoting senile cell remobilization,
It is characterized in that：The fixture block（17）It is fastened on standing groove（8）Body wall in, and fixture block（17）Surface be arc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720748693.1U CN207091415U (en) | 2017-06-26 | 2017-06-26 | With the stem cell extract detection kit for promoting senile cell remobilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720748693.1U CN207091415U (en) | 2017-06-26 | 2017-06-26 | With the stem cell extract detection kit for promoting senile cell remobilization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207091415U true CN207091415U (en) | 2018-03-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720748693.1U Active CN207091415U (en) | 2017-06-26 | 2017-06-26 | With the stem cell extract detection kit for promoting senile cell remobilization |
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| CN (1) | CN207091415U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165465A (en) * | 2018-03-27 | 2018-06-15 | 成都曌昊科技有限公司 | A kind of acquisition process kit for acquisition stem cell in tooth |
| CN111578177A (en) * | 2020-05-24 | 2020-08-25 | 刁蔷薇 | Microbial detection lighting device convenient to adjust |
-
2017
- 2017-06-26 CN CN201720748693.1U patent/CN207091415U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165465A (en) * | 2018-03-27 | 2018-06-15 | 成都曌昊科技有限公司 | A kind of acquisition process kit for acquisition stem cell in tooth |
| CN111578177A (en) * | 2020-05-24 | 2020-08-25 | 刁蔷薇 | Microbial detection lighting device convenient to adjust |
| CN111578177B (en) * | 2020-05-24 | 2021-11-09 | 上海安之诺生物科技有限公司 | Microbial detection lighting device convenient to adjust |
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