CN207114554U - A kind of kit of quick detection skin progenitor cell aging - Google Patents
A kind of kit of quick detection skin progenitor cell aging Download PDFInfo
- Publication number
- CN207114554U CN207114554U CN201720751741.2U CN201720751741U CN207114554U CN 207114554 U CN207114554 U CN 207114554U CN 201720751741 U CN201720751741 U CN 201720751741U CN 207114554 U CN207114554 U CN 207114554U
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- test board
- box body
- kit
- reagent box
- progenitor cell
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- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 24
- 230000032677 cell aging Effects 0.000 title claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 24
- 239000000428 dust Substances 0.000 claims abstract description 11
- 238000009434 installation Methods 0.000 claims abstract description 10
- 230000033001 locomotion Effects 0.000 claims abstract description 3
- 239000007787 solid Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 17
- 239000007788 liquid Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 6
- 210000002304 esc Anatomy 0.000 description 4
- 210000002615 epidermis Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 108010087230 Sincalide Proteins 0.000 description 2
- 238000010609 cell counting kit-8 assay Methods 0.000 description 2
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- 101000998011 Homo sapiens Keratin, type I cytoskeletal 19 Proteins 0.000 description 1
- 102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 description 1
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- 230000012010 growth Effects 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of kit of quick detection skin progenitor cell aging, including reagent box body, clamping device, test board and reaction cup, the reagent box body is connected with each other by movable rotation-shaft and dust cap, fix bar and installation neck are respectively fixed with the inwall of the reagent box body, the clamping device is located in fix bar, and minim pipette is installed in clamping device, the test board is located at the bottom of reagent box body, and its outside is fixed with pull handle, the reaction cup is located in the reserved fixing groove of test board, and the both sides of test board are fixed with sliding shoe, protection cushion block is installed below the reagent box body.The kit of the quick detection skin progenitor cell aging, minim pipette need not individually be picked up and reaction cup is operated in addition, the test board of activity can carry out the movement of diverse location reaction cup simultaneously, repeated detection can disposably be carried out, it is very easy to use, the speed of detection is greatly accelerated, and improves the precision of detection.
Description
Technical field
Technical field of immunoassay is the utility model is related to, specially a kind of reagent of quick detection skin progenitor cell aging
Box.
Background technology
Skin is the maximum organ of human body, and it is overlying on body surface, is made up of epidermis, corium, hypodermis and appendicle.
Resist microorganism invasion, ultraviolet radiation and prevent the loss of moisture, the appearance for adjusting body temperature and maintaining people etc. from rising
Highly important effect.Skin has extremely strong reparation and power of regeneration, and the presence of this and skin progenitor cell, which has, directly closes
System.But existing skin progenitor cell kit, can only be single deposit, when stem cell detects, it is necessary to individually pick up
Minim pipette and reaction cup are operated in addition, and use is very troublesome, influence the speed of detection.In view of the above-mentioned problems, it is badly in need of design
A kind of kit of new quick detection skin progenitor cell aging.
Utility model content
The purpose of this utility model is to provide a kind of kit of quick detection skin progenitor cell aging, above-mentioned to solve
What proposition can be single in background technology deposit, when stem cell detects, it is necessary to individually pick up minim pipette and reaction cup progress
Operation in addition, use is very troublesome, the problem of influenceing detection speed.
To achieve the above object, the utility model provides following technical scheme：A kind of quick detection skin progenitor cell aging
Kit, including reagent box body, clamping device, test board and reaction cup, the reagent box body by movable rotation-shaft with
Dust cap is connected with each other, and observation panel is provided with dust cap, and fix bar is respectively fixed with the inwall of the reagent box body
With installation neck, the clamping device is located in fix bar, and minim pipette is provided with clamping device, while its surface is provided with
Groove is extruded, the test board is located at the bottom of reagent box body, and its outside is fixed with pull handle, and the reaction cup is positioned at survey
In the reserved fixing groove of test plate (panel), and the both sides of test board are fixed with sliding shoe, and protection is provided with below the reagent box body
Cushion block.
Preferably, the clamping device includes front clamp plate, spring, rear clamping plate, anchor tip and fixed jack, preceding
Clamping plate is connected with each other by spring and rear clamping plate, and minim pipette, rear clamping plate are provided between front clamp plate and rear clamping plate
The structure being integrated with anchor tip, anchor tip are connected with each other by the reserved fixed jack in its inner side with fix bar.
Preferably, the clamping device equidistantly distributed is in fix bar.
Preferably, the horizontal level of the reaction cup is corresponding up and down with clamping device, and reaction cup is removable with fixing groove
Unload formula mounting structure.
Preferably, the sliding shoe is symmetricly set on the both sides of test board, and sliding shoe is connected with installation neck neck.
Preferably, the fixing groove is 5 × 3 arrangement architectures.
Compared with prior art, the beneficial effects of the utility model are：The reagent of the quick detection skin progenitor cell aging
Box, you can carry out the storage of reagent, extruding dropping liquid can be carried out to minim pipette by clamping device again, dropping liquid directly drops down onto test board
On reaction cup in, without individually picking up minim pipette and reaction cup and being operated in addition, while movable test board can be carried out not
With the movement of position reaction cup, repeated detection can be disposably carried out, it is very easy to use, the speed of detection is greatly accelerated, and
Improve the precision of detection, without when dust cap carry out dust-proof protection, protection cushion block improves overall stability.
Brief description of the drawings
Fig. 1 is the utility model overall structure diagram；
Fig. 2 is the utility model test board overlooking the structure diagram；
Fig. 3 is the utility model clamping device structural representation.
In figure：1st, reagent box body, 2, movable rotation-shaft, 3, dust cap, 4, observation panel, 5, fix bar, 6, installation neck, 7,
Clamping device, 701, front clamp plate, 702, spring, 703, rear clamping plate, 704, anchor tip, 705, fixed jack, 8, extruding
Groove, 9, minim pipette, 10, test board, 11, reaction cup, 12, pull handle, 13, protection cushion block, 14, sliding shoe, 15, fixing groove.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1-3 are referred to, the utility model provides a kind of technical scheme：A kind of examination of quick detection skin progenitor cell aging
Agent box, including it is reagent box body 1, movable rotation-shaft 2, dust cap 3, observation panel 4, fix bar 5, installation neck 6, clamping device 7, preceding
Clamping plate 701, spring 702, rear clamping plate 703, anchor tip 704, fixed jack 705, extruding groove 8, minim pipette 9, test board
10th, reaction cup 11, pull handle 12, protection cushion block 13, sliding shoe 14 and fixing groove 15, reagent box body 1 pass through movable rotation-shaft 2
It is connected with each other with dust cap 3, and observation panel 4 is provided with dust cap 3, fixation is respectively fixed with the inwall of reagent box body 1
Bar 5 and installation neck 6, clamping device 7 is located in fix bar 5, and minim pipette 9 is provided with clamping device 7, while its surface is set
Extruding groove 8 is equipped with, clamping device 7 includes front clamp plate 701, spring 702, rear clamping plate 703, anchor tip 704 and fixed
Jack 705, front clamp plate 701 are connected with each other by spring 702 and rear clamping plate 703, front clamp plate 701 and rear clamping plate 703
Between minim pipette 9 is installed, the structure that rear clamping plate 703 is integrated with anchor tip 704, anchor tip 704 passes through its inner side
Reserved fixed jack 705 is connected with each other with fix bar 5, realizes installation and extruding to minim pipette 9, and clamping device 7 is equidistant
It is distributed in fix bar 5, can disposably carries out multiple dropping liquid, test board 10 is located at the bottom of reagent box body 1, and its outside is solid
Surely there is pull handle 12, reaction cup 11 is located in the reserved fixing groove 15 of test board 10, and fixing groove 15 is 5 × 3 arrangement architectures, just
In follow-up multiple reaction detection, the horizontal level of reaction cup 11 and clamping device are corresponding about 7, and reaction cup 11 and fixing groove
15 be detachable installing structure, the convenient replacing and cleaning for carrying out reaction cup 11, and the both sides of test board 10 are fixed with slip
Block 14, sliding shoe 14 is symmetricly set on the both sides of test board 10, and sliding shoe 14 is connected with the installation neck of neck 6, is easy to adjust
The position of reaction cup 11, the lower section of reagent box body 1 are provided with protection cushion block 13.
Operation principle：Dust cap 3 is first turned on, minim pipette 9 is placed in clamping device 7, when needing to carry out stem cell
During detection, the extruding groove 8 in the outside of clamping device 7 is extruded with hand, now the power that is squeezed of minim pipette 9 carries out dropping liquid, and dropping liquid directly falls
In reaction cup 11 on to test board 10, due to clamping device 7 being equidistantly distributed with fix bar 5, therefore can disposably it carry out
The dropping liquid operation of multiple minim pipettes 9, and the sliding shoe 14 in the outside of test board 10 is connected with the installation neck of neck 6, operable pulling
Handle 12 pulls out test board 10 so as to realize the regulation of the position of reaction cup 11, repeats aforesaid operations by the drop in minim pipette 9
Drop enters in the reaction cup 11 of diverse location, finally takes out test board 10 completely, and different reaction solutions is instilled in reaction cup 11
Disposable repeated detection can be achieved, it is very easy to use, the speed of detection is greatly accelerated, and improve the accurate of detection
Degree, here it is the operation principle of the kit of the quick detection skin progenitor cell aging.
Experimental method：
Skin progenitor cell is separately cultured：Skin histology Iodophor soaks 5~10min, and alcohol takes off iodine.Ultraviolet disinfection works
On platform hypodermis is removed using sterile surgical apparatus.Remaining tissue is trimmed out to the skin graft of 1cm × 1cm sizes,
4 DEG C of lucifuges of Dispase enzymes separate epidermis and skin corium after digesting 12~14h, shred epidermis, 0.125% tryptose as far as possible
Enzyme（Include 0.04%EDTA）37 DEG C of 10~15min of digestion.Add complete culture solution and terminate digestion, carefully piping and druming.200 eye mesh screens
Filtering.Filtrate passes through centrifuge（1 300r/min centrifuges 5min）, abandoning supernatant, collect cell.Under inverted phase contrast microscope
Cell is observed, determines that the culture base unit weight of cell is resuspended after counting, appropriate K- SFM culture mediums is added and slowly blows and beats cell system
Into single cell suspension, insulating box is incubated overnight, and liquid is changed after 24h.It is placed in 37 DEG C, continues to cultivate in 5%CO2 incubators, later every 2
~3d changes liquid, is passed on when cell growth is fused to 70%.
Skin progenitor cell is identified：The integrins of β 1 and the identification of CK19 immunofluorescence stainings：2 generation ESCs are cultivated into 24h, used
After PBS washings cell 3 times, 4% paraformaldehyde fixes 40min, PBS 3 times；Add 0.3%Triton and carry out permeabilized processing；
After Normal Goat Serum closing 30min, topple over.Primary antibody that is disposable, adding after dilution, 4 DEG C overnight.The next day use PBS after
Add fluorescein isothiocynate（FITC）Secondary antibody is marked, lucifuge is incubated 30min.Photographed to record using inverted fluorescence microscope.
CCK-8 detection ESCs propagation：The ESCs of isolation of adult, growth selection ESCs in good condition；Pancreatin digests, from
After the heart, supernatant discarding, make cell suspension, be inoculated in 96 orifice plates, 5 × 103 cells are inoculated with per hole, respectively at 1,2,3,4,
5th, 6,7d corresponding time points take out a culture plate, accordingly make blank control group, and the μ l of CCK-8 solution 10, training are added per hole
After supporting 3h, absorbance is determined on ELIASA（A）Value, cell growth curve is drawn according to A450nm values.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of kit of quick detection skin progenitor cell aging, including reagent box body（1）, clamping device（7）, test board
（10）And reaction cup（11）, it is characterised in that：The reagent box body（1）Pass through movable rotation-shaft（2）With dust cap（3）Mutually interconnect
Connect, and dust cap（3）On be provided with observation panel（4）, the reagent box body（1）Inwall on be respectively fixed with fix bar（5）
With installation neck（6）, the clamping device（7）Positioned at fix bar（5）On, and clamping device（7）In minim pipette is installed（9）,
Its surface is provided with extruding groove simultaneously（8）, the test board（10）Positioned at reagent box body（1）Bottom, and its outside is fixed
There is pull handle（12）, the reaction cup（11）Positioned at test board（10）Reserved fixing groove（15）In, and test board（10）'s
Both sides are fixed with sliding shoe（14）, the reagent box body（1）Lower section protection cushion block is installed（13）.
A kind of 2. kit of quick detection skin progenitor cell aging according to claim 1, it is characterised in that：The folder
Tight device（7）Include front clamp plate（701）, spring（702）, rear clamping plate（703）, anchor tip（704）And fixed jack
（705）, front clamp plate（701）Pass through spring（702）With rear clamping plate（703）It is connected with each other, front clamp plate（701）With rear clamping
Plate（703）Between minim pipette is installed（9）, rear clamping plate（703）With anchor tip（704）The structure being integrated, anchor tip
（704）Pass through the reserved fixed jack in its inner side（705）With fix bar（5）It is connected with each other.
A kind of 3. kit of quick detection skin progenitor cell aging according to claim 1, it is characterised in that：The folder
Tight device（7）Equidistantly distributed is in fix bar（5）On.
A kind of 4. kit of quick detection skin progenitor cell aging according to claim 1, it is characterised in that：It is described anti-
Answer cup（11）Horizontal level and clamping device（7）Correspond to up and down, and reaction cup（11）With fixing groove（15）For detachable peace
Assembling structure.
A kind of 5. kit of quick detection skin progenitor cell aging according to claim 1, it is characterised in that：The cunning
Motion block（14）It is symmetricly set on test board（10）Both sides, and sliding shoe（14）With installing neck（6）Neck connects.
A kind of 6. kit of quick detection skin progenitor cell aging according to claim 1, it is characterised in that：It is described solid
Determine groove（15）For 5 × 3 arrangement architectures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720751741.2U CN207114554U (en) | 2017-06-26 | 2017-06-26 | A kind of kit of quick detection skin progenitor cell aging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720751741.2U CN207114554U (en) | 2017-06-26 | 2017-06-26 | A kind of kit of quick detection skin progenitor cell aging |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207114554U true CN207114554U (en) | 2018-03-16 |
Family
ID=61587611
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720751741.2U Expired - Fee Related CN207114554U (en) | 2017-06-26 | 2017-06-26 | A kind of kit of quick detection skin progenitor cell aging |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207114554U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113295684A (en) * | 2021-07-02 | 2021-08-24 | 洪岗 | Test card and manufacturing and testing method thereof |
-
2017
- 2017-06-26 CN CN201720751741.2U patent/CN207114554U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113295684A (en) * | 2021-07-02 | 2021-08-24 | 洪岗 | Test card and manufacturing and testing method thereof |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180316 |