CN212864784U

CN212864784U - Cell culture instrument for immune cell amplification

Info

Publication number: CN212864784U
Application number: CN202020686545.3U
Authority: CN
Inventors: 冯娟; 关方霞; 常菁
Original assignee: Zhongke Bosheng Bioengineering Co ltd
Current assignee: Zhongke Bosheng Bioengineering Co ltd
Priority date: 2020-04-29
Filing date: 2020-04-29
Publication date: 2021-04-02
Anticipated expiration: 2030-04-29

Abstract

The utility model discloses a cell culture appearance for immune cell amplification, including cell culture appearance body, environment intelligent regulation subassembly, trigger formula cultivation timing subassembly and disinfection subassembly, trigger formula cultivation timing subassembly is including cultivateing backup pad, timing observation display screen, first sensor, second sensor and treater. The utility model relates to a cell culture equipment technical field specifically provides a disinfect the disinfection to cultivateing the chamber through ultraviolet sterilamp to observe the corresponding regional branch on the hole is placed with the culture tube in the timing and show the time that this region cultivates regionally, and but automated inspection and control drying apparatus, humidifier and air discharge fan's work, make the internal environment reach the most suitable environment of cultivateing, be convenient for observe and use the cell culture appearance that is used for the immune cell to expand.

Description

Cell culture instrument for immune cell amplification

Technical Field

The utility model relates to a cell culture equipment technical field specifically indicates a cell culture appearance for immune cell amplification.

Background

Immune cells, commonly known as leukocytes, refer to cells involved in or associated with immune responses, including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, etc., which play an important role in the human body; the immune cell amplification technology is a process of increasing the number of immune cells in vivo and in vitro by artificially adding some factors, biological agents, traditional Chinese medicine formulas and the like, and the activity and differentiation capability of the immune cells are improved while the number is increased, so that better curative effect is exerted, and various diseases of human can be better treated; the immune cell amplification technology is an advanced cell preparation technology in the domestic regenerative biomedical industry at present, is a top-end technology of the modern service industry of the biomedical technology which greatly supports the development in China, and is also a medical innovation mode with a high-tech support foundation; china is a large population country, various major diseases are recorded in China at high incidence, and the immune cell amplification technical result can directly benefit the almost recognized 'absolute disease' patients and has extremely important significance for improving the health level of the whole people and optimizing the population structure.

The requirement for humiture, CO2 concentration are higher among the cell culture process, and the immune cell culture device on the existing market can only carry out simple control operation, and intelligent degree is not high, the setting of unable automatically regulated internal environment, and in addition, cell culture still can need to go on in batches, and probably need set up different time to cell culture with this creation experiment contrast group, but the mode of present differentiation contrast group generally is used as the mark for pasting the label, but has the shortcoming of difficult observation and inconvenient operation.

SUMMERY OF THE UTILITY MODEL

For solving the above-mentioned current difficult problem, the utility model provides a disinfect the disinfection through ultraviolet sterilamp to cultivateing the chamber to observe the display screen and cultivate the corresponding regional district on the pipe placing hole in the timing and show the time that this region cultivates regionally, and but automated inspection and control drying apparatus, humidifier and air discharge fan's work, make the internal environment reach the most suitable cultivation environment, be convenient for observe and the cell culture appearance that is used for the immune cell amplification that uses.

The utility model adopts the following technical scheme: the utility model relates to a cell culture instrument for immune cell amplification, including cell culture instrument body, environment intelligent regulation subassembly, trigger type culture timing subassembly and disinfection subassembly, the cell culture instrument body is a cavity setting with an open side, the cell culture instrument body is hinged with a sealing door at the open side, the environment intelligent regulation subassembly and the disinfection subassembly are arranged in the cell culture instrument body, the trigger type culture timing subassembly is arranged in the side wall of the cell culture instrument body and the cavity; the triggered culture timing assembly comprises a culture support plate, a timing observation display screen, a first sensor, a second sensor and a processor, wherein a partition plate is arranged in the cell culture instrument body, a culture cavity is arranged between an inner top wall and the partition plate of the cell culture instrument body, a placing cavity is arranged between the partition plate and an inner bottom wall of the cell culture instrument body, culture plate fixing assemblies are symmetrically arranged on the inner side wall of the culture cavity and comprise an upper fixing groove and a lower fixing groove, one end of the upper fixing groove is arranged on the side wall of the culture cavity in an inverted L shape, one end of the lower fixing groove is arranged on the side wall of the culture cavity in an L shape and is arranged below the upper fixing groove, the first sensor is arranged at the top end of the lower fixing groove, a culture plate sliding channel is arranged between the upper fixing groove and the lower fixing groove, and the culture plate sliding channel is arranged in a convex shape, the culture support plate is arranged in an H shape, two ends of the culture support plate are respectively inserted into the culture plate sliding channels, culture tube placing holes are uniformly formed in the culture support plate, the second sensor is arranged on the inner wall of each culture tube placing hole, the processor is arranged in the placing cavity, the processor is connected with the timing observation display screen, the first sensor and the second sensor, and the timing observation display screen is arranged on the sealing door and connected with the first sensor and the second sensor; environmental intelligent regulation subassembly includes temperature sensor, humidity transducer, CO2 consistency transmitter, humidifier, drying apparatus, exhaust fan and spray set, humidifier and drying apparatus locate and place the intracavity, spray set is connected and the other end locates the top of cultivateing the backup pad with humidifier and drying apparatus respectively, the rotatable locating of exhaust fan is on the lateral wall of cultivateing the chamber, temperature sensor, humidity transducer and CO2 consistency transmitter all are connected with the treater and locate on cultivateing the intracavity wall, drying apparatus, humidifier all are connected with temperature sensor and humidity transducer, the exhaust fan is connected with CO2 consistency transmitter.

Further, the disinfection subassembly includes the ultraviolet ray sterilamp, the ultraviolet ray sterilamp is located and is cultivateed the intracavity.

Further, the spray assembly comprises a first branch pipe, a drying valve, a second branch pipe, a humidifying valve, a main pipe and a spray head, the first branch pipe is connected with the dryer, the second branch pipe is connected with the humidifier, the drying valve is arranged on the first branch pipe, the humidifying valve is arranged on the second branch pipe, the main pipe is L-shaped and is arranged on the side wall of the culture cavity and communicated with the first branch pipe and the second branch pipe, and the spray head is communicated with the other end of the main pipe and is arranged above the culture support plate.

Further, cultivate the fixed subassembly of board and be equipped with the multiunit and evenly locate and cultivate the intracavity, the timing is observed the display screen and is connected with the same and difference of the quantity of the fixed subassembly of board, it is regional to be equipped with a plurality of groups on the timing observation display screen, regional and cultivate the backup pad on be the horizontal row and indulge the second sensor that the downthehole was placed to the culture tube of arranging in proper order corresponding, the inside timing module that is equipped with of treater, the timing module is observed the display screen with the timing and is connected.

Further, the top of cell culture appearance body is equipped with the display screen, the display screen is connected with treater, temperature sensor, humidity transducer and CO2 concentration sensor for the data condition of the inside environment of demonstration culture chamber, cell culture appearance body outside is equipped with switch button and disinfection button, the disinfection button is connected with ultraviolet sterilamp, the switch button is connected with the treater.

Furthermore, the timing observation display screen is a transparent LCD display screen, and can display timing time and observe the condition inside the culture chamber through the transparent screen.

Furthermore, the drying valve and the humidifying valve are both electromagnetic valves, the drying valve and the humidifying valve are both connected with the processor, and the processor easily controls the communication of the first branch pipe or the second branch pipe.

Furthermore, the bottom of the cell culture instrument body is provided with a support column, and the bottom of the support column is provided with a universal wheel with a brake.

Adopt above-mentioned structure the utility model discloses the beneficial effect who gains as follows: the utility model provides a cell culture appearance for immune cell augmentation, disinfect the disinfection to cultivateing the chamber through the ultraviolet ray sterilamp, place the cell culture bottle that prepares in the different culture tube placing hole of cultivateing the backup pad as required, and realize through cultivateing the backup pad and cultivate the firm connection of fixed subassembly of backup pad, cultivate the backup pad and lay and produce pressure trigger and send the signal to the treater to first sensor after accomplishing, the treater can go to read the position of the second sensor that is triggered again this moment, and observe the corresponding region timing and show with the subregion on display screen and the culture tube placing hole in the timing, temperature sensor, humidity transducer, CO2 concentration sensor can real-time detection cultivates the internal environment of chamber and sends the signal to in the treater, the work of treater automatic control drying apparatus, humidifier and air discharge fan, make the internal environment reach the most suitable cultivation environment, and can be in the convenient observation of the data condition of internal environment on the display screen, in addition because the timing is observed the display screen and is transparent LCD display screen, still can observe the condition of cultivateing the intracavity portion at any time through the timing observation display screen, avoid opening the inside environment of sealing door observation cell culture condition influence.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a cell culture apparatus for immune cell expansion according to the present invention;

FIG. 2 is an enlarged view of a portion A of FIG. 1;

fig. 3 is a partially enlarged view of a portion B in fig. 1.

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

Wherein, 1, cell culture appearance body, 2, environment intelligent regulation subassembly, 3, trigger formula cultivation timing subassembly, 4, disinfection subassembly, 5, sealing door, 6, cultivation backup pad, 7, timing observation display screen, 8, first sensor, 9, second sensor, 10, treater, 11, division board, 12, place the chamber, 13, cultivation board fixed subassembly, 14, go up fixed slot, 15, lower fixed slot, 16, cultivation board sliding channel, 17, culture tube placing hole, 18, temperature sensor, 19, humidity transducer, 20, CO2 concentration sensor, 21, humidifier, 22, drying apparatus, 23, air discharge fan, 24, spraying subassembly, 25, ultraviolet sterilizing lamp, 26, first minute pipe, 27, drying valve, 28, second minute pipe, 29, humidifying valve, 30, main pipe, 31, shower head, 32, display screen, 33, support column, 34, universal wheel, 35. culture chamber, 37, switch button, 38, disinfection button.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1-3, the utility model relates to a cell culture apparatus for immune cell expansion, which comprises a cell culture apparatus body 1, an environment intelligent adjusting component 2, a triggered culture timing component 3 and a disinfection component 4, wherein the cell culture apparatus body 1 is a hollow cavity with an open side end, a sealing door 5 is hinged at the opening of the side end of the cell culture apparatus body 1, the environment intelligent adjusting component 2 and the disinfection component 4 are arranged in the cell culture apparatus body 1, and the triggered culture timing component 3 is arranged on the side wall of the cell culture apparatus body 1 and in the hollow cavity; the triggered culture timing assembly 3 comprises a culture support plate 6, a timing observation display screen 7, a first sensor 8, a second sensor 9 and a processor 10, wherein a partition plate 11 is arranged in the cell culture instrument body 1, a culture cavity 35 is arranged between the inner top wall of the cell culture instrument body 1 and the partition plate 11, a placing cavity 12 is arranged between the partition plate 11 and the inner bottom wall of the cell culture instrument body 1, culture plate fixing assemblies 13 are symmetrically arranged on the inner side wall of the culture cavity 35, each culture plate fixing assembly 13 comprises an upper fixing groove 14 and a lower fixing groove 15, one end of each upper fixing groove 14 is arranged on the side wall of the culture cavity 35 in an inverted L shape, one end of each lower fixing groove 15 is arranged on the side wall of the culture cavity 35 and below the upper fixing groove 14, the first sensor 8 is arranged at the top end of the lower fixing groove 15, a culture plate sliding channel 16 is arranged between the upper fixing groove 14 and the lower fixing groove 15, the culture plate sliding channel 16 is arranged in a convex shape, two ends of the culture support plate 6 are arranged in an H shape and are respectively inserted into the culture plate sliding channel 16, culture tube placing holes 17 are uniformly formed in the culture support plate 6, the second sensor 9 is arranged on the inner wall of the culture tube placing hole 17, the processor 10 is arranged in the placing cavity 12, the processor 10 is connected with the timing observation display screen 7, the first sensor 8 and the second sensor 9, and the timing observation display screen 7 is arranged on the sealing door 5 and is connected with the first sensor 8 and the second sensor 9; environmental intelligent regulation subassembly 2 includes temperature sensor 18, humidity transducer 19, CO2 concentration sensor 20, humidifier 21, drying apparatus 22, exhaust fan 23 and spray set 24, humidifier 21 and drying apparatus 22 are located and are placed in chamber 12, spray set 24 is connected and the other end is located the top of cultivateing backup pad 6 with humidifier 21 and drying apparatus 22 respectively, exhaust fan 23 is rotatable to be located on the lateral wall of cultivateing chamber 35, temperature sensor 18, humidity transducer 19 and CO2 concentration sensor 20 all are connected with treater 10 and locate on cultivateing chamber 35 inner wall, drying apparatus 22, humidifier 21 all are connected with temperature sensor 18 and humidity transducer 19, the exhaust fan is connected with CO2 concentration sensor 20.

Wherein, the disinfection component 4 comprises an ultraviolet sterilization lamp 25, and the ultraviolet sterilization lamp 25 is arranged in the culture cavity 35; the spraying assembly 24 comprises a first branch pipe 26, a drying valve 27, a second branch pipe 28, a humidifying valve 29, a main pipe 30 and a spraying head 31, the first branch pipe 26 is connected with the dryer 22, the second branch pipe 28 is connected with the humidifier 21, the drying valve 27 is arranged on the first branch pipe 26, the humidifying valve 29 is arranged on the second branch pipe 28, the main pipe 30 is arranged on the side wall of the culture cavity 35 in an L shape and is communicated with the first branch pipe 26 and the second branch pipe 28, and the spraying head 31 is communicated with the other end of the main pipe 30 and is arranged above the culture support plate 6; the culture plate fixing components 13 are provided with a plurality of groups which are uniformly arranged in the culture cavity 35, the timing observation display screen 7 and the culture plate fixing components 13 are the same in number and are respectively connected, a plurality of groups of areas are arranged on the timing observation display screen 7, the areas correspond to the second sensors 9 in the culture tube placing holes 17 which are sequentially arranged in a transverse row and a longitudinal row on the culture support plate 6, a timing module is arranged in the processor 10, and the timing module is connected with the timing observation display screen 7; the top of the cell culture instrument body 1 is provided with a display screen 32, the display screen 32 is connected with the processor 10, the temperature sensor 18, the humidity sensor 19 and the CO2 concentration sensor 20, the cell culture instrument body 1 is externally provided with a switch key 37 and a disinfection key 38, the disinfection key 38 is connected with the ultraviolet sterilizing lamp 25, and the switch key 37 is connected with the processor 10; the top of the cell culture instrument body 1 is provided with a display screen 32, the display screen 32 is connected with the processor 10, the temperature sensor 18, the humidity sensor 19 and the CO2 concentration sensor 20 and is used for displaying the data condition of the internal environment of the culture cavity 35, a switch key 37 and a disinfection key 38 are arranged outside the cell culture instrument body 1, the disinfection key 38 is connected with the ultraviolet sterilizing lamp 25, and the switch key 37 is connected with the processor 10; the timing observation display screen 7 is a transparent LCD display screen; the drying valve 27 and the humidifying valve 29 are both electromagnetic valves, and the drying valve 27 and the humidifying valve 29 are both connected with the processor 10; the cell culture instrument body 1 bottom is equipped with support column 33, the universal wheel 34 of area brake is equipped with to support column 33 bottom.

When the device is used specifically, before cell culture work, the disinfection button 38 is pressed to control the ultraviolet sterilization lamp 25 to work, the culture cavity 35 is sterilized and disinfected through the ultraviolet sterilization lamp 25, after the sterilization work is finished, the disinfection button 38 is pressed again to control the ultraviolet sterilization lamp 25 to be closed, the prepared cell culture bottles are placed in the culture tube placing holes 17 of the culture support plate 6 and are arranged in different culture tube placing holes 17 according to requirements, after the placing is finished, the H-shaped two ends of the culture support plate 6 are respectively inserted in the culture plate sliding channel 16 and pushed to the inner rear wall of the culture cavity 35, because the first sensor 8 is arranged at the top end of the lower fixing groove 15, the pressure triggering is generated on the first sensor 8 after the placement of the culture support plate 6 is finished, and signals are sent to the processor 10, because the second sensor 9 is arranged on the inner wall of the culture tube placing hole 17, the processor 10 reads the position of the triggered second sensor 9, the timing observation display screen 7 is provided with a plurality of groups of areas, the areas correspond to the second sensors 9 in the culture tube placing holes 17 which are arranged in a horizontal row and a vertical row in sequence on the culture supporting plate 6, a timing module in the processor 10 can time the triggered second sensors 9 and display the triggered second sensors on the timing observation display screen 7 in regions, the temperature sensor 18, the humidity sensor 19 and the CO2 concentration sensor 20 can detect the internal environment of the culture cavity 35 in real time and send signals into the processor 10, when the humidity sensor 19 detects that the humidity is higher than the highest threshold value, the processor 10 controls the dryer 22 to work and controls the drying valve 27 to open, the drying hot air is sequentially transmitted to the main tube 20 and the spray header 31 through the first branch tube 26 to adjust the humidity, when the humidity is lower than the lowest threshold value, the humidifier 21 is controlled to work and the humidification valve 29 is opened, and the moisture is sequentially transmitted to the main tube 20 and the spray header 31 through the second branch tube 28, temperature sensor 18 detects when the temperature is higher than the highest threshold value and controls steerable humidifier 21 and convey the moisture cooling, the temperature is lower than the control desicator 22 and heats when minimum threshold value, CO2 concentration sensor 20 detects CO2 concentration and controls exhaust fan 23 work when too high, it reaches the most suitable cultivation environment to ventilate, display screen 32 and treater 10, temperature sensor 18, humidity transducer 19 and CO2 concentration sensor 20 are connected, can show the data condition of cultivateing 35 internal environment and conveniently observe, in addition because timing observation display screen 7 is transparent LCD display screen, still can observe the condition of cultivateing 35 inside at any time through timing observation display screen 7, avoid opening sealing door 5 and observe the cell culture condition and influence inside environment.

The present invention and the embodiments thereof have been described above, but the description is not limited thereto, and the embodiment shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should understand that they should not be limited to the embodiments described above, and that they can design the similar structure and embodiments without departing from the spirit of the invention.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Claims

1. A cell culture apparatus for immune cell expansion, comprising: including cell culture appearance body, environment intelligent regulation subassembly, trigger formula cultivation timing subassembly and disinfection subassembly, the cell culture appearance body is a side open-ended cavity setting, cell culture appearance body side opening part is articulated to be equipped with sealing door, this is internal for cell culture appearance is located to environment intelligent regulation subassembly and disinfection subassembly, trigger formula cultivation timing subassembly is located the cell and is banked up with earth the cell and cultivate the cellThe side wall of the nutrient body and the hollow cavity are arranged in the cavity; the triggered culture timing assembly comprises a culture support plate, a timing observation display screen, a first sensor, a second sensor and a processor, wherein a partition plate is arranged in the cell culture instrument body, a culture cavity is arranged between an inner top wall and the partition plate of the cell culture instrument body, a placing cavity is arranged between the partition plate and an inner bottom wall of the cell culture instrument body, culture plate fixing assemblies are symmetrically arranged on the inner side wall of the culture cavity and comprise an upper fixing groove and a lower fixing groove, one end of the upper fixing groove is arranged on the side wall of the culture cavity in an inverted L shape, one end of the lower fixing groove is arranged on the side wall of the culture cavity in an L shape and is arranged below the upper fixing groove, the first sensor is arranged at the top end of the lower fixing groove, a culture plate sliding channel is arranged between the upper fixing groove and the lower fixing groove, and the culture plate sliding channel is arranged in a convex shape, the culture support plate is arranged in an H shape, two ends of the culture support plate are respectively inserted into the culture plate sliding channels, culture tube placing holes are uniformly formed in the culture support plate, the second sensor is arranged on the inner wall of each culture tube placing hole, the processor is arranged in the placing cavity, the processor is connected with the timing observation display screen, the first sensor and the second sensor, and the timing observation display screen is arranged on the sealing door and connected with the first sensor and the second sensor; the environment intelligent adjusting component comprises a temperature sensor, a humidity sensor and CO₂Concentration sensor, humidifier, drying apparatus, air discharge fan and spray set, humidifier and drying apparatus are located and are placed the intracavity, spray set is connected with humidifier and drying apparatus respectively and the other end is located the top of cultivateing the backup pad, the air discharge fan is rotatable to be located on the lateral wall of cultivateing the chamber, temperature sensor, humidity transducer and CO₂Concentration sensor all is connected with the treater and locates on cultivateing the intracavity wall, drying apparatus, humidifier all are connected with temperature sensor and humidity transducer, the air discharge fan and CO₂The concentration sensor is connected.

2. A cell culture apparatus for immune cell expansion according to claim 1, wherein: the disinfection component comprises an ultraviolet sterilization lamp, and the ultraviolet sterilization lamp is arranged in the culture cavity.

3. A cell culture apparatus for immune cell expansion according to claim 1, wherein: the spray assembly comprises a first branch pipe, a drying valve, a second branch pipe, a humidifying valve, a main pipe and a spray head, the first branch pipe is connected with the dryer, the second branch pipe is connected with the humidifier, the drying valve is arranged on the first branch pipe, the humidifying valve is arranged on the second branch pipe, the main pipe is L-shaped and is arranged on the side wall of the culture cavity and communicated with the first branch pipe and the second branch pipe, and the spray head is communicated with the other end of the main pipe and is arranged above the culture support plate.

4. A cell culture apparatus for immune cell expansion according to claim 1, wherein: the fixed subassembly of training board is equipped with the multiunit and evenly locates and cultivates the intracavity, the timing is observed the display screen and is connected respectively with the same quantity of the fixed subassembly of training board, it is regional to be equipped with a plurality of groups on the timing observation display screen, it is corresponding with the culture tube placing hole's that is horizontal row and indulges row and arrange in proper order second sensor in the backup pad to be regional, the inside timing module that is equipped with of treater, the timing module is observed the display screen with the timing and is connected.

5. A cell culture apparatus for immune cell expansion according to claim 2, wherein: the top of the cell culture instrument body is provided with a display screen, and the display screen is connected with a controller, a temperature sensor, a humidity sensor and CO₂The cell culture instrument comprises a cell culture instrument body, a concentration sensor, a switch button and a disinfection button, wherein the switch button and the disinfection button are arranged outside the cell culture instrument body, the disinfection button is connected with an ultraviolet sterilization lamp, and the switch button is connected with a controller.

6. A cell culture apparatus for immune cell expansion according to claim 1, wherein: the timing observation display screen is a transparent LCD display screen.

7. A cell culture apparatus for immune cell expansion according to claim 3, wherein: the drying valve and the humidifying valve are both electromagnetic valves and are both connected with the processor.

8. A cell culture apparatus for immune cell expansion according to claim 1, wherein: the cell culture appearance body bottom is equipped with the support column, the support column bottom is equipped with the universal wheel of area brake.