CN110558232A - Tissue culture and rapid seedling growing method for paper mulberry - Google Patents
Tissue culture and rapid seedling growing method for paper mulberry Download PDFInfo
- Publication number
- CN110558232A CN110558232A CN201910994265.0A CN201910994265A CN110558232A CN 110558232 A CN110558232 A CN 110558232A CN 201910994265 A CN201910994265 A CN 201910994265A CN 110558232 A CN110558232 A CN 110558232A
- Authority
- CN
- China
- Prior art keywords
- root
- tissue culture
- root hairs
- paper mulberry
- hairs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The scheme discloses a tissue culture and rapid seedling raising method for paper mulberry in the technical field of agricultural planting, which comprises the following steps: step one, cutting root hairs: cutting the root hair of the paper mulberry, wherein the diameter of the root hair is between 0.5cm and 1cm, and soaking the root hair in a sterilization solution; step two, root hair cleaning: cleaning the soaked root hairs with clear water, and soaking the root hairs in a growth solution for 1-3 d; step three, tissue culture: cultivating root hairs in a soilless culture medium, adding a growth solution into the soilless culture medium, and soaking the bottoms of the root hairs in the growth solution; step four, transplanting: transplanting the root hairs which grow to be more than 10cm after tissue culture into a greenhouse, controlling the temperature in the greenhouse to be 28-30 ℃, and transplanting the root hairs to a field after the root hairs grow for 30 days. By adopting the root hairs to carry out seedling culture, the influence on the mother tree can be reduced.
Description
Technical Field
the invention relates to the technical field of agricultural planting, in particular to a tissue culture and rapid seedling raising method for paper mulberry.
Background
the paper mulberry is named as paper mulberry, is deciduous tree and is 10-20 m high; the paper mulberry has the characteristics of fast growth, strong adaptability, wide distribution, easy propagation, high heat quantity and short felling period, and has the advantages of shallow root system, wide lateral root distribution, fast growth, strong germination and tillering capacity, pruning resistance and strong pollution resistance. The pig feed is distributed in temperate zones and tropical zones of China, can grow no matter in plains, hills or mountains, has good leaves as pig feed, has pure white bast fiber as a high-grade raw material for papermaking, can be used for treating skin diseases by using roots and seeds as medicines, and has high economic value.
in the prior art, tender shoots or branches are usually selected for tissue culture of paper mulberry, but because the quantity of the tender shoots or the branches is limited, and the survival of the mother tree is greatly influenced by directly picking the tender shoots or the branches from the mother tree, the seedling culture method in the prior art seriously restricts the seedling culture of the paper mulberry.
disclosure of Invention
The invention aims to provide a rapid seedling culture method for tissue culture of paper mulberry, which can reduce the influence on mother trees.
The tissue culture rapid seedling method for the paper mulberry in the scheme comprises the following steps:
Step one, cutting root hairs: cutting the root hair of the paper mulberry, wherein the diameter of the root hair is between 0.5cm and 1cm, and soaking the root hair in a sterilization solution;
step two, root hair cleaning: cleaning the soaked root hairs with clear water, and soaking the root hairs in a growth solution for 1-3 d;
Step three, tissue culture: cultivating root hairs in a soilless culture medium, adding a growth solution into the soilless culture medium, and soaking the bottoms of the root hairs in the growth solution;
step four, transplanting: transplanting the root hairs which grow to be more than 10cm after tissue culture into a greenhouse, controlling the temperature in the greenhouse to be 28-30 ℃, and transplanting the root hairs to a field after the root hairs grow for 30 days.
Further, the sterilization liquid is prepared by diluting thiophanate methyl wettable powder by 1000 times with water. The thiophanate methyl wettable powder consists of thiophanate methyl, a surfactant and a carrier, absorbs a low-toxicity sterilization solution in a broad spectrum, and has the functions of absorption, prevention and treatment, so that the incision can be healed while the incision is sterilized by adopting the sterilization solution, and the survival rate of root hairs is increased.
further, in the step one, the incision of the root hair needs to be wrapped by gauze when the root hair is soaked. Utilize the gauze parcel incision, the gauze can play certain effect of blockking, can prevent that more antiseptic solution active ingredient from entering into the inside of root hair, influencing the survival rate of root hair.
further, after 4-8 parts of calcium superphosphate, 4-8 parts of monoammonium phosphate, 4-8 parts of ferrous sulfate and 4-8 parts of copper sulfate are mixed, 20-30 parts of moringa seed extracting solution is added. The moringa seed extracting solution can be subjected to complexation with trace elements such as Ga, Cu, Cl, Fe and the like, and a complex of the moringa seed extracting solution has good water solubility and stability, can solve the problem of nutrient elements lacking in roots and can stably supply nutrient elements.
Further, the green house in the fourth step is covered by a black film. The black film is used for reducing the illumination intensity of the greenhouse, so that the survival rate of the root hairs is increased.
further, the temperature in the greenhouse is 29 ℃. The growth rate of the paper mulberry is high at the temperature.
Compared with the prior art, the seedling raising method has the advantages that the root hairs are adopted for seedling raising, and the seedling raising has little influence on the survival of the paper mulberry because the paper mulberry contains more root hairs and the root hairs with larger diameter are not cut.
Detailed Description
The following is further detailed by the specific embodiments:
Example 1: a tissue culture and rapid seedling method for paper mulberry comprises the following steps:
Step one, cutting root hairs: cutting the root hair of the paper mulberry, wherein the diameter of the root hair is between 0.5, soaking the root hair in a sterilizing solution, wherein the incision of the root hair needs to be wrapped by gauze when the root hair is soaked, and the sterilizing solution is prepared by diluting thiophanate methyl wettable powder by 1000 times with water;
step two, root hair cleaning: cleaning the soaked root hair with clear water, soaking the root hair in a growth solution, mixing the growth solution with 4 parts of calcium superphosphate, 4 parts of monoammonium phosphate, 4 parts of ferrous sulfate and 4 parts of copper sulfate, adding 20 parts of moringa seed extracting solution, and soaking for 1 d;
Step three, tissue culture: cultivating root hairs in a soilless culture medium, adding a growth solution into the soilless culture medium, and soaking the bottoms of the root hairs in the growth solution;
Step four, transplanting: transplanting the root hairs which grow to be more than 10cm after tissue culture into a greenhouse, covering the greenhouse with a black film in the fourth step, controlling the temperature in the greenhouse to be 29 ℃, and transplanting the root hairs to a field after the root hairs grow for 30 days.
example 2:
A tissue culture and rapid seedling method for paper mulberry comprises the following steps:
step one, cutting root hairs: cutting the root hair of the paper mulberry, wherein the diameter of the root hair is between 0.75cm, soaking the root hair in a sterilizing solution, wherein the incision of the root hair needs to be wrapped by gauze when the root hair is soaked, and the sterilizing solution is prepared by diluting thiophanate methyl wettable powder by 1000 times with water;
step two, root hair cleaning: cleaning the soaked root hair with clear water, soaking the root hair in a growth solution, mixing the growth solution with 6 parts of calcium superphosphate, 5 parts of monoammonium phosphate, 6 parts of ferrous sulfate and 6 parts of copper sulfate, adding 25 parts of moringa seed extracting solution, and soaking for 2 days;
step three, tissue culture: cultivating root hairs in a soilless culture medium, adding a growth solution into the soilless culture medium, and soaking the bottoms of the root hairs in the growth solution;
Step four, transplanting: transplanting the root hairs which grow to be more than 10cm after tissue culture into a greenhouse, covering the greenhouse with a black film in the fourth step, controlling the temperature in the greenhouse to be 29 ℃, and transplanting the root hairs to a field after the root hairs grow for 30 days.
example 3:
A tissue culture and rapid seedling method for paper mulberry comprises the following steps:
step one, cutting root hairs: cutting the root hair of the paper mulberry, wherein the diameter of the root hair is 1cm, soaking the root hair in a sterilizing solution, wherein the incision of the root hair needs to be wrapped by gauze when the root hair is soaked, and the sterilizing solution is prepared by diluting thiophanate methyl wettable powder by 1000 times with water;
Step two, root hair cleaning: cleaning the soaked root hair with clear water, soaking the root hair in a growth solution, mixing the growth solution with 8 parts of calcium superphosphate, 8 parts of monoammonium phosphate, 8 parts of ferrous sulfate and 8 parts of copper sulfate, adding 30 parts of moringa seed extracting solution, and soaking for 3 days;
Step three, tissue culture: cultivating root hairs in a soilless culture medium, adding a growth solution into the soilless culture medium, and soaking the bottoms of the root hairs in the growth solution;
Step four, transplanting: transplanting the root hairs which grow to be more than 10cm after tissue culture into a greenhouse, covering the greenhouse with a black film in the fourth step, controlling the temperature in the greenhouse to be 29 ℃, and transplanting the root hairs to a field after the root hairs grow for 30 days.
In the above examples, 1 part was 1 kg. Compared with the prior art, the root hairs are adopted for seedling culture, and the broussonetia papyrifera contains more root hairs, and the root hairs with larger diameters are not cut during seedling culture, so that the influence on the survival of the broussonetia papyrifera is small.
Claims (6)
1. a tissue culture and rapid seedling method for paper mulberry is characterized in that: the method comprises the following steps:
Step one, cutting root hairs: cutting the root hair of the paper mulberry, wherein the diameter of the root hair is between 0.5cm and 1cm, and soaking the root hair in a sterilization solution;
step two, root hair cleaning: cleaning the soaked root hairs with clear water, and soaking the root hairs in a growth solution for 1-3 d;
Step three, tissue culture: cultivating root hairs in a soilless culture medium, adding a growth solution into the soilless culture medium, and soaking the bottoms of the root hairs in the growth solution;
step four, transplanting: transplanting the root hairs which grow to be more than 10cm after tissue culture into a greenhouse, controlling the temperature in the greenhouse to be 28-30 ℃, and transplanting the root hairs to a field after the root hairs grow for 30 days.
2. the tissue culture and rapid seedling raising method for paper mulberry according to claim 1, which is characterized in that: the sterilization liquid is prepared by diluting thiophanate methyl wettable powder 1000 times by water.
3. The tissue culture and rapid seedling raising method for paper mulberry according to claim 2, which is characterized in that: and in the first step, the incision of the root hair needs to be wrapped by gauze when the root hair is soaked.
4. The tissue culture and rapid seedling raising method for paper mulberry according to claim 3, which is characterized in that: the growth liquid is prepared by mixing 4-8 parts of calcium superphosphate, 4-8 parts of monoammonium phosphate, 4-8 parts of ferrous sulfate and 4-8 parts of copper sulfate, and adding 20-30 parts of moringa seed extracting solution.
5. The tissue culture and rapid seedling raising method of the paper mulberry according to claim 4, which is characterized in that: and in the fourth step, the greenhouse is covered by a black film.
6. The tissue culture and rapid seedling raising method for paper mulberry according to claim 5, which is characterized in that: the temperature in the greenhouse is 29 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910994265.0A CN110558232A (en) | 2019-10-18 | 2019-10-18 | Tissue culture and rapid seedling growing method for paper mulberry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910994265.0A CN110558232A (en) | 2019-10-18 | 2019-10-18 | Tissue culture and rapid seedling growing method for paper mulberry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110558232A true CN110558232A (en) | 2019-12-13 |
Family
ID=68785306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910994265.0A Pending CN110558232A (en) | 2019-10-18 | 2019-10-18 | Tissue culture and rapid seedling growing method for paper mulberry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110558232A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753393A (en) * | 2020-12-31 | 2021-05-07 | 华南农业大学 | Efficient broussonetia papyrifera root propagation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220803A (en) * | 2018-10-31 | 2019-01-18 | 胡荣山 | A kind of hybridization paper mulberry tissue culture adventitious bud outside sprout-cultivating-bottle and method for culturing seedlings |
-
2019
- 2019-10-18 CN CN201910994265.0A patent/CN110558232A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220803A (en) * | 2018-10-31 | 2019-01-18 | 胡荣山 | A kind of hybridization paper mulberry tissue culture adventitious bud outside sprout-cultivating-bottle and method for culturing seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 王羽梅: "《芳香蔬菜》", 30 September 2018, 华中科技大学出版社 * |
| 褚天铎等: "《简明施肥技术手册》", 31 January 2014, 金盾出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753393A (en) * | 2020-12-31 | 2021-05-07 | 华南农业大学 | Efficient broussonetia papyrifera root propagation method |
| CN112753393B (en) * | 2020-12-31 | 2022-04-22 | 华南农业大学 | Broussonetia papyrifera root propagation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102612961B (en) | Method for cultivating olive seedlings | |
| CN103621289A (en) | Tomato soilless culture method | |
| CN104126386A (en) | Potato breeder's seed production method | |
| CN105940893B (en) | The fast breeding method of fruit of Rangoon creeper's seedling | |
| CN107667817A (en) | The control flower method of Bougainvillea spectabilis | |
| CN104303812A (en) | Eggplant planting method | |
| CN102007863A (en) | Cherry root stock cutting and grafting method | |
| CN101263768A (en) | Louis iris plant division replication fast seedling establishment and ecological application method | |
| CN103875515A (en) | Ex-vitro rooting method for tissue culture blueberry seedlings | |
| CN106856711A (en) | A kind of method for promoting apocarya seed quick-speed germination | |
| RU2448453C1 (en) | Method of stimulation development, growth and productivity of plants | |
| CN113348954A (en) | Sweet cherry root zone limited cultivation method in warm and humid regions in south | |
| CN107996242A (en) | A kind of Moringa container seedling culture method | |
| CN104160850A (en) | Cultivation method for dwarfing potted zinnia elegans | |
| CN104221669B (en) | A kind of purple Chinese yam nursery and cultivation method | |
| CN110558232A (en) | Tissue culture and rapid seedling growing method for paper mulberry | |
| CN104396511A (en) | Tomato seedling breeding technology | |
| CN107211820A (en) | A kind of Cuiguan pear cuttage planting method for improving yield | |
| CN104756713A (en) | Method for quickly breeding acer rubrum based on cutting technique | |
| CN105340665A (en) | Highly-efficient cultivation method of myrciaria cauliflora | |
| CN105519419A (en) | Method for propagating twigs of acer palmatum Jinling Haungfeng by cutting | |
| CN113455266A (en) | Seedling raising method for extra-large apple type fragrant papaya | |
| CN111727802A (en) | Novel cultivation method of Chinese toon sprouts | |
| CN107409886A (en) | A kind of breeding method of dragon fruit | |
| CN112166974A (en) | Tomato seedling culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ran Xian Inventor after: Niu Jiangrong Inventor before: Ran Xian |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191213 |