CN110547191B - Seed cultivation method for improving salt tolerance of common head cabbage - Google Patents
Seed cultivation method for improving salt tolerance of common head cabbage Download PDFInfo
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- 244000178937 Brassica oleracea var. capitata Species 0.000 title claims abstract description 22
- 230000015784 hyperosmotic salinity response Effects 0.000 title claims abstract description 13
- 238000012364 cultivation method Methods 0.000 title claims abstract description 11
- 239000003104 tissue culture media Substances 0.000 claims abstract description 24
- 235000015097 nutrients Nutrition 0.000 claims abstract description 18
- 238000005286 illumination Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- 230000010355 oscillation Effects 0.000 claims abstract description 16
- 241000241413 Propolis Species 0.000 claims abstract description 9
- 229940069949 propolis Drugs 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 16
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 15
- 239000004202 carbamide Substances 0.000 claims description 15
- 229920001817 Agar Polymers 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 239000008272 agar Substances 0.000 claims description 10
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 5
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 5
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 5
- 235000003704 aspartic acid Nutrition 0.000 claims description 5
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 5
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 5
- 239000006012 monoammonium phosphate Substances 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 230000005059 dormancy Effects 0.000 abstract description 5
- 230000007226 seed germination Effects 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 239000003513 alkali Substances 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 240000007124 Brassica oleracea Species 0.000 description 4
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 4
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 4
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 239000002686 phosphate fertilizer Substances 0.000 description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229960000304 folic acid Drugs 0.000 description 2
- 235000019152 folic acid Nutrition 0.000 description 2
- 239000011724 folic acid Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- CCBICDLNWJRFPO-UHFFFAOYSA-N 2,6-dichloroindophenol Chemical compound C1=CC(O)=CC=C1N=C1C=C(Cl)C(=O)C(Cl)=C1 CCBICDLNWJRFPO-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- -1 potassium sulfate compound Chemical class 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of common head cabbage seed cultivation, in particular to a seed cultivation method for improving salt tolerance of common head cabbage, which comprises the following steps: s1 steam treatment: smearing propolis with thickness of 1-3mm on the seeds, and treating in steam environment at 120-130 deg.C for 10-15 s; s2 dark culture: putting the seeds into a tissue culture medium A for dark culture; s3 oscillation process: placing the seeds in a nutrient solution to vertically oscillate for 10-20min, wherein the oscillation frequency is 100-; s4 light culture: placing the seeds in a tissue culture medium B for illumination culture; by adopting the method provided by the invention, the dormancy of the seeds can be rapidly and effectively released, the dormancy period is shortened to about 30 days, the seed germination is accelerated, and the seed germination rate is improved.
Description
Technical Field
The invention relates to the technical field of common head cabbage seed cultivation, in particular to a seed cultivation method for improving salt tolerance of common head cabbage.
Background
China is a big world saline-alkali land, and the saline-alkali soil area accounts for about 1.03 percent of the national soil area, so that the saline-alkali land improvement causes the wide planting of the country and even the world, and the economic benefit of planting plants is the highest in a plurality of improvement measures. Therefore, the present applicant tried to improve saline-alkali soil by using common head cabbage.
The common head cabbage is a plant of cruciferae and brassica, is a variety of cabbages, is also called cabbage, pimple white, cabbage, lotus white, small iron heads and the like, has the characteristics of cold resistance, disease resistance, strong adaptability, easy storage, transportation resistance, high yield, dark green to green leaves, smooth leaf surfaces, thick leaf flesh, good taste, rich nutrition and the like, and is popular with consumers; the common head cabbage flower bolt is extremely rich in nutrition, the content of vitamin C is as high as 1040.00mg/kg (FW), the content of various mineral substances is high, the flower bolt is crisp, tender and delicious, the skin is removed when the flower bolt is eaten, the flower bolt can be eaten cooked or uncooked, and the fresh, tender and tasty flower bolt is eaten and has excellent mouthfeel. The common head cabbage is temperate in nature and climate, can resist low temperature and high temperature, the appropriate temperature in the common head period is 18-20 ℃, the adaptive temperature range is 7-25 ℃, but the common head cabbage is not suitable for being planted in saline-alkali soil, and the content of vitamin C in the common head cabbage can be influenced.
Disclosure of Invention
The invention provides a seed cultivation method for improving the salt tolerance of common head cabbages to solve the technical problems.
The method is realized by the following technical scheme:
a seed cultivation method for improving salt tolerance of common head cabbages comprises the following steps:
s1 steam treatment: smearing propolis with thickness of 1-3mm on the seeds, and treating in steam environment at 120-130 deg.C for 10-15 s;
s2 dark culture: putting the seeds into a tissue culture medium A for dark culture;
s3 oscillation process: placing the seeds in a nutrient solution to vertically oscillate for 10-20min, wherein the oscillation frequency is 100-;
s4 light culture: placing the seeds into a tissue culture medium B for illumination culture.
The tissue culture medium A: 1/2MS +0.8mg/L6-BA +1.2mg/L LNAA +25g/L sucrose +5g/L agar.
The working conditions of the dark culture are as follows: the culture time is 5-8h, and the culture temperature is 15-25 ℃.
The nutrient solution comprises the following raw materials in parts by weight: 3-5 parts of monoammonium phosphate, 2-4 parts of calcium nitrate, 7-9 parts of urea, 1-2 parts of 6-BA, 1-2 parts of aspartic acid and 1.5-2.5 parts of activated carbon.
The tissue culture medium B: MS +0.5mg/LNAA +1.0mg/LIBA +30g/L sucrose +6g/L agar.
The working conditions of the illumination culture are as follows: the culture time is 18-20h, the culture temperature is 25-30 ℃, and the illumination intensity is 2500-.
Has the advantages that:
(1) by adopting the method provided by the invention, the dormancy of the seeds can be rapidly and effectively released, the dormancy period is shortened to about 30 days, the seed germination is accelerated, and the seed germination rate is improved.
(2) According to the method provided by the invention, the seeds can be subjected to steam treatment to open pores of the seeds, and the growth of harmful bacteria is effectively inhibited by combining dark culture, so that the germination of the seeds is facilitated; the oscillation treatment is combined, so that the permeation rate of the nutrient solution is effectively controlled, the balance of effective components in seed cells is ensured, and the permeability of the seeds is enhanced; finally, the content of effective components in the seeds is adjusted by utilizing illumination culture, the salt tolerance of the seeds is effectively improved, the activity of the seeds is excited, and the germination rate of the seeds is high and the dormancy period is short.
The invention uses propolis to smear seeds, effectively prevents the loss of effective components in the seeds, adds urea and active carbon in nutrient solution, and then cooperates with oscillation to change the crystal structure of the urea, so that the urea and the active carbon can be mutually matched, and the urea and the active carbon can be coated on the surface layers of the seeds to continuously absorb nutrients in soil for the planting of the seeds.
Detailed Description
The following is a detailed description of the embodiments of the present invention, but the present invention is not limited to these embodiments, and any modifications or substitutions in the basic spirit of the embodiments are included in the scope of the present invention as claimed in the claims.
Example 1
A seed cultivation method for improving salt tolerance of common head cabbages comprises the following steps:
s1 steam treatment: smearing propolis with thickness of 3mm on the seeds, and treating for 15s in 130 deg.C steam environment;
s2 dark culture: putting the seeds into a tissue culture medium A for dark culture;
s3 oscillation process: placing the seeds in a nutrient solution, and vertically oscillating for 20min, wherein the oscillation frequency is 300 times/min;
s4 light culture: placing the seeds into a tissue culture medium B for illumination culture.
The tissue culture medium A: 1/2MS +0.8mg/L6-BA +1.2mg/L LNAA +25g/L sucrose +5g/L agar.
The working conditions of the dark culture are as follows: the culture time is 8h, and the culture temperature is 25 ℃.
The nutrient solution comprises the following raw materials in parts by weight: 5 parts of monoammonium phosphate, 4 parts of calcium nitrate, 9 parts of urea, 2 parts of 6-BA, 2 parts of aspartic acid and 2.5 parts of activated carbon.
The tissue culture medium B: MS +0.5mg/LNAA +1.0mg/LIBA +30g/L sucrose +6g/L agar.
The working conditions of the illumination culture are as follows: the culture time is 20h, the culture temperature is 30 ℃, and the illumination intensity is 3000 lx.
Example 2
A seed cultivation method for improving salt tolerance of common head cabbages comprises the following steps:
s1 steam treatment: smearing propolis with thickness of 1mm on the seeds, and treating in 120 deg.C steam environment for 10 s;
s2 dark culture: putting the seeds into a tissue culture medium A for dark culture;
s3 oscillation process: placing the seeds in a nutrient solution, and vertically oscillating for 10min, wherein the oscillation frequency is 100 times/min;
s4 light culture: placing the seeds into a tissue culture medium B for illumination culture.
The tissue culture medium A: 1/2MS +0.8mg/L6-BA +1.2mg/L LNAA +25g/L sucrose +5g/L agar.
The working conditions of the dark culture are as follows: the culture time is 5h, and the culture temperature is 15 ℃.
The nutrient solution comprises the following raw materials in parts by weight: 3 parts of monoammonium phosphate, 2 parts of calcium nitrate, 7 parts of urea, 1 part of 6-BA, 1 part of aspartic acid and 1.5 parts of activated carbon.
The tissue culture medium B: MS +0.5mg/LNAA +1.0mg/LIBA +30g/L sucrose +6g/L agar.
The working conditions of the illumination culture are as follows: the culture time is 18h, the culture temperature is 25 ℃, and the illumination intensity is 2500 lx.
Example 3
A seed cultivation method for improving salt tolerance of common head cabbages comprises the following steps:
s1 steam treatment: smearing propolis with thickness of 2mm on the seeds, and treating for 12s in steam environment at 125 deg.C;
s2 dark culture: putting the seeds into a tissue culture medium A for dark culture;
s3 oscillation process: placing the seeds in a nutrient solution, and vertically oscillating for 15min, wherein the oscillation frequency is 200 times/min;
s4 light culture: placing the seeds into a tissue culture medium B for illumination culture.
The tissue culture medium A: 1/2MS +0.8mg/L6-BA +1.2mg/L LNAA +25g/L sucrose +5g/L agar.
The working conditions of the dark culture are as follows: the culture time is 6.5h, and the culture temperature is 20 ℃.
The nutrient solution comprises the following raw materials in parts by weight: 4 parts of monoammonium phosphate, 3 parts of calcium nitrate, 8 parts of urea, 1.5 parts of 6-BA, 1.5 parts of aspartic acid and 2 parts of activated carbon.
The tissue culture medium B: MS +0.5mg/LNAA +1.0mg/LIBA +30g/L sucrose +6g/L agar.
The working conditions of the illumination culture are as follows: the culture time was 19h, the culture temperature was 28 ℃ and the light intensity was 2800 lx.
Comparative example 1
The difference from example 3 is that: active carbon and urea are not added into the nutrient solution.
Comparative example 2
The difference from example 3 is that: the nutrient solution is not added with urea.
Comparative example 3
The difference from example 3 is that: activated carbon is not added into the nutrient solution.
Comparative example 4
The difference from example 3 is that: propolis is not applied.
Comparative example 5
The difference from example 3 is that: light culture was excluded.
Comparative example 6
A seed cultivation method for improving salt tolerance of common head cabbages comprises the following steps:
s1 steam treatment: smearing propolis with thickness of 2mm on the seeds, and treating for 12s in steam environment at 125 deg.C;
s2 oscillation process: placing the seeds in a nutrient solution, and vertically oscillating for 15min, wherein the oscillation frequency is 200 times/min;
s3 dark culture: putting the seeds into a tissue culture medium A for dark culture;
s4 light culture: placing the seeds into a tissue culture medium B for illumination culture.
Test example 1
The common head cabbage seeds are treated according to the methods of the comparative examples and the embodiments, then salt stress treatment is carried out, and the salt tolerance of the seeds is observed, wherein the test method comprises the following steps: after the seeds are treated by the method of the comparative example and the embodiment, the seeds are placed in glass tissue culture bottles with 6 layers of filter paper and the diameter of 7cm, 40 cabbage seeds are placed in each tissue culture bottle, and the steps are repeated for 3 times; adding 3mL of NaCl solution with different concentrations (the concentrations are 0%, 0.2%, 0.4%, 0.8% and 1.2% respectively) into each tissue culture bottle, tightly covering the bottle cap, and growing for 4d in a 28 ℃ greenhouse under normal illumination (10000 lx); the number of the seeds which germinate for 1 time is recorded by uncovering every day, and the germination rate of the seeds is counted; the results are shown in Table 1;
TABLE 1 comparison of the germination rates of the common head cabbage groups
The test is carried out in 2017 at 6 months, common head cabbage seeds are treated according to the method of the comparative example and the embodiment, and the planting area of each area is 3m2(ii) a The planting method comprises the following steps: 500g of potassium sulfate compound fertilizer is applied to each area, and after herbicide is sprayed, mulching films are covered, and then the mulching films are transplanted. After field planting, fertilizing for 5 times, and applying 30g of urea to each cell at the 1 st time; applying 30g of urea and 30g of phosphate fertilizer to each cell at the 2 nd time; applying 30g of urea and 30g of phosphate fertilizer to each cell at the 3 rd time; 35g of urea and 35g of phosphate fertilizer are applied every time at the 4 th time; applying 45g of phosphate fertilizer to each area at the 5 th time, and measuring the content of the vitamin C in 5 months in 2018 according to a 2, 6-dichloroindophenol titration method in GB 5009.86-2016 (determination of ascorbic acid in food); the folic acid content was determined by liquid chromatography according to the influence of cooking and storage on folic acid content of eight common leafy vegetables (published in 2018 by Liangying et al). The results are shown in Table 2;
TABLE 2
Claims (3)
1. A seed cultivation method for improving salt tolerance of common head cabbages is characterized by comprising the following steps:
s1 steam treatment: smearing propolis with thickness of 1-3mm on the seeds, and treating in steam environment at 120-130 deg.C for 10-15 s;
s2 dark culture: putting the seeds into a tissue culture medium A for dark culture;
s3 oscillation process: placing the seeds in a nutrient solution to vertically oscillate for 10-20min, wherein the oscillation frequency is 100-;
s4 light culture: placing the seeds in a tissue culture medium B for illumination culture;
the tissue culture medium A: 1/2MS +0.8mg/L6-BA +1.2mg/L LNAA +25g/L sucrose +5g/L agar;
the nutrient solution is prepared from the following raw materials in parts by weight: 3-5 parts of monoammonium phosphate, 2-4 parts of calcium nitrate, 7-9 parts of urea, 1-2 parts of 6-BA, 1-2 parts of aspartic acid and 1.5-2.5 parts of activated carbon;
the tissue culture medium B: MS +0.5mg/LNAA +1.0mg/LIBA +30g/L sucrose +6g/L agar.
2. The method for cultivating seeds of claim 1, wherein the dark cultivation is performed under the following conditions: the culture time is 5-8h, and the culture temperature is 15-25 ℃.
3. The method of claim 1, wherein the light cultivation is performed under the following conditions: the culture time is 18-20h, the culture temperature is 25-30 ℃, and the illumination intensity is 2500-.
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